Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050201 | MS.gene91473 | 0.800108 | 1.68E-48 | -1.69E-46 |
| MS.gene050807 | MS.gene91473 | 0.801359 | 9.35E-49 | -1.69E-46 |
| MS.gene053054 | MS.gene91473 | -0.800525 | 1.38E-48 | -1.69E-46 |
| MS.gene057060 | MS.gene91473 | 0.800583 | 1.35E-48 | -1.69E-46 |
| MS.gene05954 | MS.gene91473 | 0.805094 | 1.58E-49 | -1.69E-46 |
| MS.gene060634 | MS.gene91473 | -0.824329 | 8.56E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 9 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAACGGAAGAAGAATTTCT+TGG | 0.371083 | 6.1:+4613702 | MS.gene91473:CDS |
| TTTGACAGGTTTGAGTGTAT+TGG | 0.406027 | 6.1:+4613860 | MS.gene91473:CDS |
| GCATAGCATAGGAGCTTCAT+TGG | 0.413800 | 6.1:+4613830 | MS.gene91473:intron |
| TTATACTTGAGAGTGAGGTT+TGG | 0.476020 | 6.1:-4613917 | None:intergenic |
| ATTAACCTCATCGTAATCTT+CGG | 0.481625 | 6.1:-4613504 | None:intergenic |
| AAATGTTATACTTGAGAGTG+AGG | 0.512491 | 6.1:-4613922 | None:intergenic |
| GGTTGAAGAATATGAAGAAA+CGG | 0.528087 | 6.1:+4613686 | MS.gene91473:CDS |
| TTGTTTAAATTAGGACTCTG+AGG | 0.606909 | 6.1:+4613665 | MS.gene91473:intron |
| TGACTCCGAAGATTACGATG+AGG | 0.719719 | 6.1:+4613499 | MS.gene91473:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTCAAAACATAAAAAA+TGG | - | chr6.1:4614130-4614149 | None:intergenic | 10.0% |
| !! | AAATTCAAAACATAAAAAAT+GGG | - | chr6.1:4614129-4614148 | None:intergenic | 10.0% |
| !!! | TTTGATTTTATATAGATTTA+AGG | + | chr6.1:4614067-4614086 | MS.gene91473:intron | 10.0% |
| !! | AATTCAAAACATAAAAAATG+GGG | - | chr6.1:4614128-4614147 | None:intergenic | 15.0% |
| !! | CTATATAAAATCAAAGTTAT+AGG | - | chr6.1:4614062-4614081 | None:intergenic | 15.0% |
| !! | TCAAATCTTGAAATTTATAT+TGG | + | chr6.1:4613791-4613810 | MS.gene91473:intron | 15.0% |
| !!! | AATTTATATTGGTTGAAATG+AGG | + | chr6.1:4613802-4613821 | MS.gene91473:intron | 20.0% |
| !!! | ATTTATATTGGTTGAAATGA+GGG | + | chr6.1:4613803-4613822 | MS.gene91473:intron | 20.0% |
| !!! | TTTATTATTGTTTTCAACAG+TGG | + | chr6.1:4614148-4614167 | MS.gene91473:intron | 20.0% |
| !!! | TTAACAGTTCTAAATCATGT+GGG | + | chr6.1:4613641-4613660 | MS.gene91473:intron | 25.0% |
| !!! | TTTAACAGTTCTAAATCATG+TGG | + | chr6.1:4613640-4613659 | MS.gene91473:intron | 25.0% |
| !!! | TTTTGAAACTATTTGAGTTG+TGG | + | chr6.1:4613745-4613764 | MS.gene91473:intron | 25.0% |
| AAATGTTATACTTGAGAGTG+AGG | - | chr6.1:4613925-4613944 | None:intergenic | 30.0% | |
| AGTTATAGGATATGAATGCA+CGG | - | chr6.1:4614048-4614067 | None:intergenic | 30.0% | |
| ATTAACCTCATCGTAATCTT+CGG | - | chr6.1:4613507-4613526 | None:intergenic | 30.0% | |
| CTGAAAAACCAGAAGAAAAT+GGG | - | chr6.1:4614013-4614032 | None:intergenic | 30.0% | |
| GGTTGAAGAATATGAAGAAA+CGG | + | chr6.1:4613686-4613705 | MS.gene91473:CDS | 30.0% | |
| TGCACAAAATAGTGAATACA+TGG | - | chr6.1:4613955-4613974 | None:intergenic | 30.0% | |
| TTGTTTAAATTAGGACTCTG+AGG | + | chr6.1:4613665-4613684 | MS.gene91473:intron | 30.0% | |
| ! | AATTATGTCCCATTTTCTTC+TGG | + | chr6.1:4614002-4614021 | MS.gene91473:intron | 30.0% |
| ! | CATGTGGGTTTGTTTAAATT+AGG | + | chr6.1:4613656-4613675 | MS.gene91473:intron | 30.0% |
| ! | TATATCTCCTTTTGTCTACA+AGG | + | chr6.1:4613574-4613593 | MS.gene91473:intron | 30.0% |
| CATAGAACCTTGTAGACAAA+AGG | - | chr6.1:4613584-4613603 | None:intergenic | 35.0% | |
| CCTGAAAAACCAGAAGAAAA+TGG | - | chr6.1:4614014-4614033 | None:intergenic | 35.0% | |
| GAAACGGAAGAAGAATTTCT+TGG | + | chr6.1:4613702-4613721 | MS.gene91473:CDS | 35.0% | |
| TGGGACATAATTCAGTCAAA+TGG | - | chr6.1:4613994-4614013 | None:intergenic | 35.0% | |
| TTATACTTGAGAGTGAGGTT+TGG | - | chr6.1:4613920-4613939 | None:intergenic | 35.0% | |
| TTTGACAGGTTTGAGTGTAT+TGG | + | chr6.1:4613860-4613879 | MS.gene91473:CDS | 35.0% | |
| !! | TCATTGGATGTGATTTTGAC+AGG | + | chr6.1:4613846-4613865 | MS.gene91473:CDS | 35.0% |
| !!! | CCATTTTCTTCTGGTTTTTC+AGG | + | chr6.1:4614011-4614030 | MS.gene91473:intron | 35.0% |
| ATGAGGGTAAAGCATAGCAT+AGG | + | chr6.1:4613819-4613838 | MS.gene91473:intron | 40.0% | |
| CACAGGGCACAATATAAGTA+AGG | - | chr6.1:4614098-4614117 | None:intergenic | 40.0% | |
| AAAAATGGGGCGCAAATCAC+AGG | - | chr6.1:4614115-4614134 | None:intergenic | 45.0% | |
| AAAATGGGGCGCAAATCACA+GGG | - | chr6.1:4614114-4614133 | None:intergenic | 45.0% | |
| GCATAGCATAGGAGCTTCAT+TGG | + | chr6.1:4613830-4613849 | MS.gene91473:intron | 45.0% | |
| TGACTCCGAAGATTACGATG+AGG | + | chr6.1:4613499-4613518 | MS.gene91473:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 4613480 | 4614186 | 4613480 | ID=MS.gene91473 |
| chr6.1 | mRNA | 4613480 | 4614186 | 4613480 | ID=MS.gene91473.t1;Parent=MS.gene91473 |
| chr6.1 | exon | 4613480 | 4613520 | 4613480 | ID=MS.gene91473.t1.exon1;Parent=MS.gene91473.t1 |
| chr6.1 | CDS | 4613480 | 4613520 | 4613480 | ID=cds.MS.gene91473.t1;Parent=MS.gene91473.t1 |
| chr6.1 | exon | 4613678 | 4613723 | 4613678 | ID=MS.gene91473.t1.exon2;Parent=MS.gene91473.t1 |
| chr6.1 | CDS | 4613678 | 4613723 | 4613678 | ID=cds.MS.gene91473.t1;Parent=MS.gene91473.t1 |
| chr6.1 | exon | 4613836 | 4613933 | 4613836 | ID=MS.gene91473.t1.exon3;Parent=MS.gene91473.t1 |
| chr6.1 | CDS | 4613836 | 4613933 | 4613836 | ID=cds.MS.gene91473.t1;Parent=MS.gene91473.t1 |
| chr6.1 | exon | 4614168 | 4614186 | 4614168 | ID=MS.gene91473.t1.exon4;Parent=MS.gene91473.t1 |
| chr6.1 | CDS | 4614168 | 4614186 | 4614168 | ID=cds.MS.gene91473.t1;Parent=MS.gene91473.t1 |
| Gene Sequence |
| Protein sequence |
