AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene91663

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91663.t1 MTR_7g013140 94.805 231 12 0 2 232 21 251 1.08e-166 459
MS.gene91663.t1 MTR_7g013150 85.217 230 34 0 3 232 24 253 1.00e-150 419
MS.gene91663.t1 MTR_7g013120 77.391 230 51 1 4 232 24 253 2.36e-135 380
MS.gene91663.t1 MTR_8g058990 75.000 228 57 0 5 232 26 253 5.26e-130 367
MS.gene91663.t1 MTR_8g464350 73.246 228 61 0 5 232 27 254 4.49e-127 360
MS.gene91663.t1 MTR_7g061720 61.261 222 84 1 9 228 32 253 2.47e-97 285
MS.gene91663.t1 MTR_5g079950 59.459 222 88 1 9 228 33 254 1.71e-96 282
MS.gene91663.t1 MTR_2g097030 59.910 222 87 1 9 228 34 255 4.48e-96 281
MS.gene91663.t1 MTR_3g094060 61.261 222 82 2 9 228 31 250 7.29e-96 280
MS.gene91663.t1 MTR_3g094040 61.261 222 82 2 9 228 31 250 1.13e-95 280
MS.gene91663.t1 MTR_3g435430 59.910 222 85 2 9 228 25 244 4.25e-93 273
MS.gene91663.t1 MTR_5g075320 58.744 223 88 2 9 229 29 249 1.84e-92 272
MS.gene91663.t1 MTR_5g041700 58.590 227 90 2 4 228 20 244 6.45e-92 270
MS.gene91663.t1 MTR_3g466760 60.360 222 84 2 9 228 38 257 3.81e-91 269
MS.gene91663.t1 MTR_3g466790 60.360 222 84 2 9 228 32 251 1.06e-90 268
MS.gene91663.t1 MTR_4g063975 59.009 222 89 1 9 228 32 253 2.36e-90 267
MS.gene91663.t1 MTR_4g081950 57.207 222 91 2 9 228 61 280 2.23e-89 265
MS.gene91663.t1 MTR_7g011060 56.889 225 89 2 4 228 21 237 3.35e-87 258
MS.gene91663.t1 MTR_1g085460 57.848 223 88 4 9 226 30 251 1.08e-85 255
MS.gene91663.t1 MTR_1g026020 55.263 228 97 3 9 232 29 255 1.82e-84 252
MS.gene91663.t1 MTR_4g124340 51.111 225 105 3 8 229 60 282 6.94e-83 249
MS.gene91663.t1 MTR_2g090255 50.442 226 110 1 6 229 25 250 7.62e-83 248
MS.gene91663.t1 MTR_0040s0140 50.885 226 109 1 6 229 29 254 7.70e-83 248
MS.gene91663.t1 MTR_5g076950 54.018 224 100 2 8 229 40 262 1.62e-81 244
MS.gene91663.t1 MTR_7g111010 52.838 229 102 4 9 232 27 254 2.50e-81 244
MS.gene91663.t1 MTR_7g110990 52.838 229 102 4 9 232 12 239 3.33e-81 243
MS.gene91663.t1 MTR_5g011210 52.632 228 104 3 8 232 36 262 1.32e-79 239
MS.gene91663.t1 MTR_6g012520 57.727 220 85 2 9 228 28 239 8.58e-78 234
MS.gene91663.t1 MTR_4g102450 51.101 227 107 3 9 232 36 261 7.47e-76 230
MS.gene91663.t1 MTR_3g064510 47.436 234 108 6 8 229 52 282 6.75e-70 216
MS.gene91663.t1 MTR_0008s0120 48.246 228 62 2 5 232 28 199 2.66e-69 211
MS.gene91663.t1 MTR_2g090270 32.500 240 138 8 5 232 31 258 4.80e-31 115
MS.gene91663.t1 MTR_2g090265 32.218 239 138 9 6 232 30 256 4.02e-30 112
MS.gene91663.t1 MTR_0122s0020 32.500 240 138 8 5 232 37 264 9.08e-29 109
MS.gene91663.t1 MTR_6g012500 51.961 102 49 0 127 228 176 277 4.93e-27 105
MS.gene91663.t1 MTR_0809s0010 31.120 241 134 11 8 232 46 270 7.88e-24 96.7
MS.gene91663.t1 MTR_4g028640 30.864 243 134 13 6 232 40 264 5.25e-21 89.0
MS.gene91663.t1 MTR_4g099370 28.761 226 142 9 11 227 30 245 1.01e-19 85.1
MS.gene91663.t1 MTR_5g013440 29.237 236 136 11 12 232 32 251 1.47e-18 82.0
MS.gene91663.t1 MTR_4g075790 28.750 240 136 10 6 229 20 240 3.29e-18 81.3
MS.gene91663.t1 MTR_0118s0070 27.350 234 144 9 14 232 53 275 5.15e-18 80.9
MS.gene91663.t1 MTR_0122s0020 32.768 177 96 7 5 170 37 201 6.13e-17 76.6
MS.gene91663.t1 MTR_1g085365 54.902 51 23 0 176 226 4 54 3.47e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91663.t1 AT5G39280 67.249 229 73 1 4 232 49 275 1.72e-118 339
MS.gene91663.t1 AT5G39300 67.686 229 72 1 4 232 50 276 3.79e-118 338
MS.gene91663.t1 AT5G39290 66.667 228 74 1 5 232 54 279 2.23e-116 333
MS.gene91663.t1 AT5G39270 67.105 228 73 1 5 232 54 279 4.44e-116 333
MS.gene91663.t1 AT5G02260 61.712 222 83 1 9 228 32 253 1.75e-101 295
MS.gene91663.t1 AT5G39310 62.667 225 81 2 9 232 90 312 1.86e-101 297
MS.gene91663.t1 AT2G39700 61.261 222 84 1 9 228 31 252 8.29e-98 286
MS.gene91663.t1 AT2G37640 60.360 222 86 1 9 228 36 257 1.83e-97 285
MS.gene91663.t1 AT5G56320 60.352 227 86 2 4 228 24 248 3.56e-96 281
MS.gene91663.t1 AT5G56320 60.352 227 86 2 4 228 24 248 3.56e-96 281
MS.gene91663.t1 AT5G56320 60.352 227 86 2 4 228 24 248 2.43e-95 281
MS.gene91663.t1 AT5G39260 59.227 233 87 3 1 232 37 262 4.92e-95 279
MS.gene91663.t1 AT2G40610 59.740 231 88 3 1 228 22 250 6.96e-94 276
MS.gene91663.t1 AT3G55500 57.848 223 92 1 8 228 33 255 2.22e-93 275
MS.gene91663.t1 AT5G05290 61.435 223 81 3 9 228 32 252 6.76e-93 273
MS.gene91663.t1 AT2G03090 58.590 227 90 2 4 228 25 249 7.62e-93 273
MS.gene91663.t1 AT1G69530 58.559 222 88 2 9 228 27 246 2.73e-92 271
MS.gene91663.t1 AT1G69530 58.559 222 88 2 9 228 27 246 2.73e-92 271
MS.gene91663.t1 AT2G28950 58.559 222 90 1 9 228 31 252 1.07e-91 270
MS.gene91663.t1 AT1G69530 58.636 220 87 2 9 226 27 244 1.67e-91 270
MS.gene91663.t1 AT2G37640 60.000 210 82 1 21 228 23 232 1.53e-90 266
MS.gene91663.t1 AT3G29030 58.182 220 85 2 9 228 38 250 9.48e-88 260
MS.gene91663.t1 AT1G26770 55.895 229 97 2 2 228 19 245 8.14e-87 258
MS.gene91663.t1 AT1G26770 55.895 229 97 2 2 228 29 255 1.45e-86 257
MS.gene91663.t1 AT1G12560 51.982 227 104 4 9 232 37 261 2.51e-84 251
MS.gene91663.t1 AT4G01630 52.966 236 105 4 2 232 21 255 9.50e-83 247
MS.gene91663.t1 AT1G20190 53.982 226 99 3 11 232 28 252 8.06e-81 242
MS.gene91663.t1 AT3G03220 54.260 223 99 2 8 228 39 260 9.84e-81 243
MS.gene91663.t1 AT1G62980 49.780 227 110 3 9 232 31 256 7.51e-79 238
MS.gene91663.t1 AT1G69530 60.221 181 68 2 9 187 27 205 8.40e-77 232
MS.gene91663.t1 AT1G69530 60.221 181 68 2 9 187 27 205 8.57e-77 231
MS.gene91663.t1 AT4G38210 43.290 231 127 2 3 229 22 252 3.77e-72 220
MS.gene91663.t1 AT3G15370 46.696 227 113 5 9 231 27 249 9.78e-67 206
MS.gene91663.t1 AT1G20190 53.368 193 85 3 44 232 2 193 4.35e-66 203
MS.gene91663.t1 AT3G15370 47.596 208 101 5 28 231 76 279 7.66e-61 192
MS.gene91663.t1 AT1G65680 33.058 242 135 11 6 232 44 273 3.34e-27 105
MS.gene91663.t1 AT4G28250 31.667 240 139 9 6 232 33 260 2.25e-26 103
MS.gene91663.t1 AT2G20750 32.353 238 139 10 8 232 39 267 1.45e-24 98.6
MS.gene91663.t1 AT4G28250 30.962 239 136 9 6 232 33 254 2.79e-24 97.4
MS.gene91663.t1 AT2G45110 28.992 238 138 9 12 232 36 259 1.64e-20 87.4
MS.gene91663.t1 AT1G65681 29.464 224 133 8 22 232 11 222 1.82e-20 86.7
MS.gene91663.t1 AT3G60570 26.891 238 148 7 8 232 33 257 1.25e-17 79.7
MS.gene91663.t1 AT3G45970 28.767 219 122 9 28 228 15 217 1.26e-16 76.6
MS.gene91663.t1 AT4G17030 26.724 232 155 8 5 229 23 246 1.41e-14 71.2
MS.gene91663.t1 AT4G28250 29.714 175 96 7 6 170 33 190 4.63e-14 68.9
MS.gene91663.t1 AT3G45970 27.907 215 121 9 32 228 46 244 5.70e-14 69.7
MS.gene91663.t1 AT4G28250 30.286 175 95 8 6 170 33 190 7.82e-14 68.6
MS.gene91663.t1 AT2G20750 31.609 174 99 8 8 170 39 203 1.49e-13 67.8
MS.gene91663.t1 AT3G45960 29.082 196 106 9 50 228 16 195 1.42e-12 65.1
MS.gene91663.t1 AT3G45960 28.037 214 121 9 32 228 46 243 1.76e-12 65.5

Find 61 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCTTCAAATTGGCAATT+TGG 0.158518 7.4:-86146538 MS.gene91663:CDS
GCATGAGCATCGTACCAATT+TGG 0.200212 7.4:+86147508 None:intergenic
TTGGAATATCGGAACGAATT+TGG 0.227218 7.4:-86146626 MS.gene91663:CDS
CTCCTTCAAATTGGCAATTT+GGG 0.273683 7.4:-86146537 MS.gene91663:CDS
ATTCAGTTTCTCCTTCAAAT+TGG 0.302042 7.4:-86146546 MS.gene91663:CDS
GGTTCCCATGTCACGCAATT+GGG 0.338945 7.4:-86146656 MS.gene91663:CDS
CATGAGCATCGTACCAATTT+GGG 0.339274 7.4:+86147509 None:intergenic
GCAGAGGGAGCTTGTGGTTA+TGG 0.345450 7.4:-86147108 MS.gene91663:intron
TGGCTGGCATTGACCCAAAT+TGG 0.353682 7.4:-86147522 MS.gene91663:CDS
GGAACACGTCGATATTTAAC+AGG 0.354155 7.4:+86146808 None:intergenic
TTTGTTGCGGTTATCGTGAT+AGG 0.354832 7.4:+86146949 None:intergenic
AGCACTGCTCTATTCAATAA+TGG 0.360305 7.4:-86147042 MS.gene91663:CDS
GGGTTCCCATGTCACGCAAT+TGG 0.368675 7.4:-86146657 MS.gene91663:CDS
CGACCCTCAATGGTGCATAA+AGG 0.376417 7.4:-86146980 MS.gene91663:CDS
TCAGTGGTCTTGCTGTAATC+TGG 0.378673 7.4:+86146916 None:intergenic
CAATTTGGGCAGACCTTTGA+GGG 0.397290 7.4:-86146523 MS.gene91663:CDS
GTGTTCAATGTTGCCAATGC+TGG 0.419882 7.4:-86146721 MS.gene91663:CDS
ACCCGCTGCATCACCGTAGA+AGG 0.421455 7.4:+86147483 None:intergenic
ACAGCAAGACCACTGACATT+TGG 0.434617 7.4:-86146909 MS.gene91663:CDS
CACCCTTTATGCACCATTGA+GGG 0.435760 7.4:+86146977 None:intergenic
GACCCTCAATGGTGCATAAA+GGG 0.438140 7.4:-86146979 MS.gene91663:CDS
TTCCGTCACTTGTAGTAACT+TGG 0.438292 7.4:+86146584 None:intergenic
GATCTCTTCAAACAAGGGTA+TGG 0.441783 7.4:-86147084 MS.gene91663:CDS
GATGCTCATGCAACCTTCTA+CGG 0.442027 7.4:-86147496 MS.gene91663:CDS
AGTATCAAAGGGTCTAATAC+TGG 0.447111 7.4:-86146682 MS.gene91663:CDS
CTCCTCGTAAAGGGCATGGC+TGG 0.450828 7.4:-86147538 None:intergenic
CGTGTTCCATGCATCAAAAC+CGG 0.453167 7.4:-86146793 MS.gene91663:CDS
GGGCAGACCTTTGAGGGCAA+TGG 0.465089 7.4:-86146517 MS.gene91663:CDS
TGTAGTAACTTGGAACGATA+AGG 0.468509 7.4:+86146594 None:intergenic
GTTTCTCGTGTTAGTATCAA+AGG 0.473402 7.4:-86146694 MS.gene91663:CDS
TTCAATAATGGATTTACTTG+TGG 0.476193 7.4:-86147030 MS.gene91663:CDS
GCAATTTGGGCAGACCTTTG+AGG 0.481360 7.4:-86146524 MS.gene91663:CDS
TGCCCAAATTGCCAATTTGA+AGG 0.491435 7.4:+86146535 None:intergenic
CTTGTGTCGACGACCCTCAA+TGG 0.494578 7.4:-86146990 MS.gene91663:CDS
AGGGTATGGGCTTGCAACTA+CGG 0.497920 7.4:-86147070 MS.gene91663:CDS
GCACCCTTTATGCACCATTG+AGG 0.509796 7.4:+86146976 None:intergenic
TTGTTGCGGTTATCGTGATA+GGG 0.519560 7.4:+86146950 None:intergenic
ACCTTCTACGGTGATGCAGC+GGG 0.524229 7.4:-86147484 MS.gene91663:CDS
GTTCCCATGTCACGCAATTG+GGG 0.527294 7.4:-86146655 MS.gene91663:CDS
GTGTTCCATGCATCAAAACC+GGG 0.527511 7.4:-86146792 MS.gene91663:CDS
AAAATTTCCATTGCCCTCAA+AGG 0.530553 7.4:+86146510 None:intergenic
ATCTCTTCAAACAAGGGTAT+GGG 0.533065 7.4:-86147083 MS.gene91663:CDS
TCAAAGGGTCTAATACTGGA+TGG 0.543308 7.4:-86146678 MS.gene91663:CDS
AATATCGGAACGAATTTGGT+AGG 0.565838 7.4:-86146622 MS.gene91663:CDS
GGTGTTAGGTTTGAAATCAA+AGG 0.569053 7.4:-86146769 MS.gene91663:CDS
ACGAGAAACATCTCCAGCAT+TGG 0.571727 7.4:+86146708 None:intergenic
TTTCTCGTGTTAGTATCAAA+GGG 0.575473 7.4:-86146693 MS.gene91663:CDS
CAAAGGGTCTAATACTGGAT+GGG 0.581086 7.4:-86146677 MS.gene91663:CDS
TTCCAAGTTACTACAAGTGA+CGG 0.582146 7.4:-86146586 MS.gene91663:CDS
TATTATGCAGAGGGAGCTTG+TGG 0.589305 7.4:-86147114 MS.gene91663:intron
AACCTTCTACGGTGATGCAG+CGG 0.592492 7.4:-86147485 MS.gene91663:CDS
GATTTCAAACCTAACACCCC+CGG 0.594844 7.4:+86146774 None:intergenic
TATGGTGATCTCTTCAAACA+AGG 0.598795 7.4:-86147090 MS.gene91663:CDS
TTCCCATGTCACGCAATTGG+GGG 0.644287 7.4:-86146654 MS.gene91663:CDS
GTGGTCTTGCTGTAATCTGG+AGG 0.654551 7.4:+86146919 None:intergenic
TTCTACGGTGATGCAGCGGG+TGG 0.667920 7.4:-86147481 MS.gene91663:CDS
AGGGTTGCACCAAATGTCAG+TGG 0.668476 7.4:+86146900 None:intergenic
ATGGTGATCTCTTCAAACAA+GGG 0.669728 7.4:-86147089 MS.gene91663:CDS
TGTTCCATGCATCAAAACCG+GGG 0.684812 7.4:-86146791 MS.gene91663:CDS
GTTCCATGCATCAAAACCGG+GGG 0.706331 7.4:-86146790 MS.gene91663:CDS
GAACACGTCGATATTTAACA+GGG 0.741865 7.4:+86146809 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATTTATTAGTAAATACA+AGG - chr7.4:86146721-86146740 MS.gene91663:CDS 10.0%
!!! GATTTTTTTTTTTTTTGTTG+AGG - chr7.4:86146858-86146877 MS.gene91663:CDS 15.0%
! ACCAAAACAAGCAAAAAATT+AGG + chr7.4:86147294-86147313 None:intergenic 25.0%
! ATATAGTTATAACTTACGCA+TGG + chr7.4:86146583-86146602 None:intergenic 25.0%
! ATATTTGGTATATTATGCAG+AGG - chr7.4:86146909-86146928 MS.gene91663:CDS 25.0%
! CCAAAACAAGCAAAAAATTA+GGG + chr7.4:86147293-86147312 None:intergenic 25.0%
! TATTTGGTATATTATGCAGA+GGG - chr7.4:86146910-86146929 MS.gene91663:CDS 25.0%
!!! TTCAATAATGGATTTACTTG+TGG - chr7.4:86147003-86147022 MS.gene91663:CDS 25.0%
ATTCAGTTTCTCCTTCAAAT+TGG - chr7.4:86147487-86147506 MS.gene91663:CDS 30.0%
TTTCTCGTGTTAGTATCAAA+GGG - chr7.4:86147340-86147359 MS.gene91663:intron 30.0%
!! CTCAAATCAAAGTGTTTTTG+CGG + chr7.4:86147159-86147178 None:intergenic 30.0%
!!! CCCTAATTTTTTGCTTGTTT+TGG - chr7.4:86147290-86147309 MS.gene91663:intron 30.0%
AAAATTTCCATTGCCCTCAA+AGG + chr7.4:86147526-86147545 None:intergenic 35.0%
AATATCGGAACGAATTTGGT+AGG - chr7.4:86147411-86147430 MS.gene91663:intron 35.0%
AGCACTGCTCTATTCAATAA+TGG - chr7.4:86146991-86147010 MS.gene91663:CDS 35.0%
AGTATCAAAGGGTCTAATAC+TGG - chr7.4:86147351-86147370 MS.gene91663:intron 35.0%
ATGGTGATCTCTTCAAACAA+GGG - chr7.4:86146944-86146963 MS.gene91663:CDS 35.0%
ATTGAGAACAATTGCATGCA+TGG - chr7.4:86146770-86146789 MS.gene91663:CDS 35.0%
CTCCTTCAAATTGGCAATTT+GGG - chr7.4:86147496-86147515 MS.gene91663:CDS 35.0%
GAACACGTCGATATTTAACA+GGG + chr7.4:86147227-86147246 None:intergenic 35.0%
GTTTCTCGTGTTAGTATCAA+AGG - chr7.4:86147339-86147358 MS.gene91663:intron 35.0%
TATGGTGATCTCTTCAAACA+AGG - chr7.4:86146943-86146962 MS.gene91663:CDS 35.0%
TCTCCTTCAAATTGGCAATT+TGG - chr7.4:86147495-86147514 MS.gene91663:CDS 35.0%
TGTAGTAACTTGGAACGATA+AGG + chr7.4:86147442-86147461 None:intergenic 35.0%
TTCCAAGTTACTACAAGTGA+CGG - chr7.4:86147447-86147466 MS.gene91663:intron 35.0%
TTGGAATATCGGAACGAATT+TGG - chr7.4:86147407-86147426 MS.gene91663:intron 35.0%
! ATCTCTTCAAACAAGGGTAT+GGG - chr7.4:86146950-86146969 MS.gene91663:CDS 35.0%
! GAGGGCAATGGAAATTTTTA+GGG - chr7.4:86147528-86147547 MS.gene91663:CDS 35.0%
! GGTGTTAGGTTTGAAATCAA+AGG - chr7.4:86147264-86147283 MS.gene91663:intron 35.0%
! TGAGGGCAATGGAAATTTTT+AGG - chr7.4:86147527-86147546 MS.gene91663:CDS 35.0%
!! AAATCAAAGTGTTTTTGCGG+AGG + chr7.4:86147156-86147175 None:intergenic 35.0%
!! AATCAAAGTGTTTTTGCGGA+GGG + chr7.4:86147155-86147174 None:intergenic 35.0%
!! TGGAGGACAAAAATTTGTTG+CGG + chr7.4:86147100-86147119 None:intergenic 35.0%
CAAAGGGTCTAATACTGGAT+GGG - chr7.4:86147356-86147375 MS.gene91663:intron 40.0%
GATCTCTTCAAACAAGGGTA+TGG - chr7.4:86146949-86146968 MS.gene91663:CDS 40.0%
GGAACACGTCGATATTTAAC+AGG + chr7.4:86147228-86147247 None:intergenic 40.0%
TCAAAGGGTCTAATACTGGA+TGG - chr7.4:86147355-86147374 MS.gene91663:intron 40.0%
TCTATTGCCTACGAAAAAGC+AGG - chr7.4:86147195-86147214 MS.gene91663:intron 40.0%
TGCCCAAATTGCCAATTTGA+AGG + chr7.4:86147501-86147520 None:intergenic 40.0%
TGGGGGCAAAATTGGAATAT+CGG - chr7.4:86147396-86147415 MS.gene91663:intron 40.0%
TTCCGTCACTTGTAGTAACT+TGG + chr7.4:86147452-86147471 None:intergenic 40.0%
TTGTTGCGGTTATCGTGATA+GGG + chr7.4:86147086-86147105 None:intergenic 40.0%
TTTGTTGCGGTTATCGTGAT+AGG + chr7.4:86147087-86147106 None:intergenic 40.0%
! CATGAGCATCGTACCAATTT+GGG + chr7.4:86146527-86146546 None:intergenic 40.0%
!!! TGCTTTTTCGTAGGCAATAG+AGG + chr7.4:86147196-86147215 None:intergenic 40.0%
ACAGCAAGACCACTGACATT+TGG - chr7.4:86147124-86147143 MS.gene91663:intron 45.0%
ACGAGAAACATCTCCAGCAT+TGG + chr7.4:86147328-86147347 None:intergenic 45.0%
CAATTTGGGCAGACCTTTGA+GGG - chr7.4:86147510-86147529 MS.gene91663:CDS 45.0%
CACCCTTTATGCACCATTGA+GGG + chr7.4:86147059-86147078 None:intergenic 45.0%
CGTGTTCCATGCATCAAAAC+CGG - chr7.4:86147240-86147259 MS.gene91663:intron 45.0%
GACCCTCAATGGTGCATAAA+GGG - chr7.4:86147054-86147073 MS.gene91663:CDS 45.0%
GATGCTCATGCAACCTTCTA+CGG - chr7.4:86146537-86146556 MS.gene91663:CDS 45.0%
GATTTCAAACCTAACACCCC+CGG + chr7.4:86147262-86147281 None:intergenic 45.0%
GTGTTCAATGTTGCCAATGC+TGG - chr7.4:86147312-86147331 MS.gene91663:intron 45.0%
GTGTTCCATGCATCAAAACC+GGG - chr7.4:86147241-86147260 MS.gene91663:intron 45.0%
TATTATGCAGAGGGAGCTTG+TGG - chr7.4:86146919-86146938 MS.gene91663:CDS 45.0%
TGTTCCATGCATCAAAACCG+GGG - chr7.4:86147242-86147261 MS.gene91663:intron 45.0%
! GCATGAGCATCGTACCAATT+TGG + chr7.4:86146528-86146547 None:intergenic 45.0%
! TCAGTGGTCTTGCTGTAATC+TGG + chr7.4:86147120-86147139 None:intergenic 45.0%
!!! GACGATACCTGCTTTTTCGT+AGG + chr7.4:86147205-86147224 None:intergenic 45.0%
!!! TGCATGGCTGCTACTTTTCT+TGG - chr7.4:86146786-86146805 MS.gene91663:CDS 45.0%
!!! CTTTTTATATAAATTATATT+TGG - chr7.4:86146894-86146913 MS.gene91663:CDS 5.0%
AACCTTCTACGGTGATGCAG+CGG - chr7.4:86146548-86146567 MS.gene91663:CDS 50.0%
AGGGTATGGGCTTGCAACTA+CGG - chr7.4:86146963-86146982 MS.gene91663:CDS 50.0%
AGGGTTGCACCAAATGTCAG+TGG + chr7.4:86147136-86147155 None:intergenic 50.0%
CACGCAATTGGGGGCAAAAT+TGG - chr7.4:86147388-86147407 MS.gene91663:intron 50.0%
CGACCCTCAATGGTGCATAA+AGG - chr7.4:86147053-86147072 MS.gene91663:CDS 50.0%
GCAATTTGGGCAGACCTTTG+AGG - chr7.4:86147509-86147528 MS.gene91663:CDS 50.0%
GCACCCTTTATGCACCATTG+AGG + chr7.4:86147060-86147079 None:intergenic 50.0%
GGTTCCCATGTCACGCAATT+GGG - chr7.4:86147377-86147396 MS.gene91663:intron 50.0%
GTTCCATGCATCAAAACCGG+GGG - chr7.4:86147243-86147262 MS.gene91663:intron 50.0%
GTTCCCATGTCACGCAATTG+GGG - chr7.4:86147378-86147397 MS.gene91663:intron 50.0%
TTCCCATGTCACGCAATTGG+GGG - chr7.4:86147379-86147398 MS.gene91663:intron 50.0%
! GTGGTCTTGCTGTAATCTGG+AGG + chr7.4:86147117-86147136 None:intergenic 50.0%
!! TGGCTGGCATTGACCCAAAT+TGG - chr7.4:86146511-86146530 MS.gene91663:CDS 50.0%
ACCTTCTACGGTGATGCAGC+GGG - chr7.4:86146549-86146568 MS.gene91663:CDS 55.0%
GCAGAGGGAGCTTGTGGTTA+TGG - chr7.4:86146925-86146944 MS.gene91663:CDS 55.0%
GGGTTCCCATGTCACGCAAT+TGG - chr7.4:86147376-86147395 MS.gene91663:intron 55.0%
TGCCCCCAATTGCGTGACAT+GGG + chr7.4:86147384-86147403 None:intergenic 55.0%
TTGCCCCCAATTGCGTGACA+TGG + chr7.4:86147385-86147404 None:intergenic 55.0%
! CTTGTGTCGACGACCCTCAA+TGG - chr7.4:86147043-86147062 MS.gene91663:CDS 55.0%
!! ACACCCCCGGTTTTGATGCA+TGG + chr7.4:86147249-86147268 None:intergenic 55.0%
!! GCATCAAAACCGGGGGTGTT+AGG - chr7.4:86147250-86147269 MS.gene91663:intron 55.0%
ACCCGCTGCATCACCGTAGA+AGG + chr7.4:86146553-86146572 None:intergenic 60.0%
TTCTACGGTGATGCAGCGGG+TGG - chr7.4:86146552-86146571 MS.gene91663:CDS 60.0%
! GGGCAGACCTTTGAGGGCAA+TGG - chr7.4:86147516-86147535 MS.gene91663:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 86146507 86147548 86146507 ID=MS.gene91663
chr7.4 mRNA 86146507 86147548 86146507 ID=MS.gene91663.t1;Parent=MS.gene91663
chr7.4 exon 86147470 86147548 86147470 ID=MS.gene91663.t1.exon1;Parent=MS.gene91663.t1
chr7.4 CDS 86147470 86147548 86147470 ID=cds.MS.gene91663.t1;Parent=MS.gene91663.t1
chr7.4 exon 86146507 86147126 86146507 ID=MS.gene91663.t1.exon2;Parent=MS.gene91663.t1
chr7.4 CDS 86146507 86147126 86146507 ID=cds.MS.gene91663.t1;Parent=MS.gene91663.t1
Gene Sequence

>MS.gene91663

GGCATGGCTGGCATTGACCCAAATTGGTACGATGCTCATGCAACCTTCTACGGTGATGCAGCGGGTGGTGAAACCATGCGTAAGTTATAACTATATTCTCTTTGAATTTTTTTTTTTTTTGAATATCAATTGTTAAATGAAGACAAGTTGTTAATAAAATTTAACAGTATATTTAAATTTAAATATACTATCAATTGTTAAAAAAAATTCATAGTATATTTATTAGTAAATACAAGGTTGTCTAAATTTCATCTAAACTAAAAATTGAGAACAATTGCATGCATGGCTGCTACTTTTCTTGGTAACATTGATCTCATGCACAATTATAAAAAAAATCTTGTTTAATTGTTTGATTTTTTTTTTTTTTGTTGAGGTTGTTTCATTACGCTTTTTATATAAATTATATTTGGTATATTATGCAGAGGGAGCTTGTGGTTATGGTGATCTCTTCAAACAAGGGTATGGGCTTGCAACTACGGCGTTAAGCACTGCTCTATTCAATAATGGATTTACTTGTGGTGCATGTTTTCAGATAACTTGTGTCGACGACCCTCAATGGTGCATAAAGGGTGCAAGCCCTATCACGATAACCGCAACAAATTTTTGTCCTCCAGATTACAGCAAGACCACTGACATTTGGTGCAACCCTCCGCAAAAACACTTTGATTTGAGTTATAAAATGTTCACCTCTATTGCCTACGAAAAAGCAGGTATCGTCCCTGTTAAATATCGACGTGTTCCATGCATCAAAACCGGGGGTGTTAGGTTTGAAATCAAAGGAAACCCTAATTTTTTGCTTGTTTTGGTGTTCAATGTTGCCAATGCTGGAGATGTTTCTCGTGTTAGTATCAAAGGGTCTAATACTGGATGGGTTCCCATGTCACGCAATTGGGGGCAAAATTGGAATATCGGAACGAATTTGGTAGGACAAGCCTTATCGTTCCAAGTTACTACAAGTGACGGAAAAACTTTAGAGTTAGATTCAGTTTCTCCTTCAAATTGGCAATTTGGGCAGACCTTTGAGGGCAATGGAAATTTTTAG
Protein sequence

>MS.gene91663.t1

GMAGIDPNWYDAHATFYGDAAGGETMQGACGYGDLFKQGYGLATTALSTALFNNGFTCGACFQITCVDDPQWCIKGASPITITATNFCPPDYSKTTDIWCNPPQKHFDLSYKMFTSIAYEKAGIVPVKYRRVPCIKTGGVRFEIKGNPNFLLVLVFNVANAGDVSRVSIKGSNTGWVPMSRNWGQNWNIGTNLVGQALSFQVTTSDGKTLELDSVSPSNWQFGQTFEGNGNF