Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28934.t1 | XP_013456587.1 | 95 | 260 | 13 | 0 | 1 | 260 | 1 | 260 | 9.40E-140 | 506.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28934.t1 | Q9SVE5 | 64.4 | 247 | 86 | 1 | 16 | 260 | 19 | 265 | 3.9e-94 | 345.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28934.t1 | I3SMJ2 | 95.0 | 260 | 13 | 0 | 1 | 260 | 1 | 260 | 6.8e-140 | 506.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene91660 | MS.gene28934 | PPI |
| MS.gene72362 | MS.gene28934 | PPI |
| MS.gene057481 | MS.gene28934 | PPI |
| MS.gene97207 | MS.gene28934 | PPI |
| MS.gene003384 | MS.gene28934 | PPI |
| MS.gene54620 | MS.gene28934 | PPI |
| MS.gene72003 | MS.gene28934 | PPI |
| MS.gene29929 | MS.gene28934 | PPI |
| MS.gene09712 | MS.gene28934 | PPI |
| MS.gene070610 | MS.gene28934 | PPI |
| MS.gene011582 | MS.gene28934 | PPI |
| MS.gene015374 | MS.gene28934 | PPI |
| MS.gene21522 | MS.gene28934 | PPI |
| MS.gene046098 | MS.gene28934 | PPI |
| MS.gene97204 | MS.gene28934 | PPI |
| MS.gene021857 | MS.gene28934 | PPI |
| MS.gene97202 | MS.gene28934 | PPI |
| MS.gene91663 | MS.gene28934 | PPI |
| MS.gene021859 | MS.gene28934 | PPI |
| MS.gene071212 | MS.gene28934 | PPI |
| MS.gene021858 | MS.gene28934 | PPI |
| MS.gene61273 | MS.gene28934 | PPI |
| MS.gene057479 | MS.gene28934 | PPI |
| MS.gene071213 | MS.gene28934 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28934.t1 | MTR_4g075790 | 98.077 | 260 | 5 | 0 | 1 | 260 | 1 | 260 | 0.0 | 531 |
| MS.gene28934.t1 | MTR_4g075790 | 97.326 | 187 | 5 | 0 | 74 | 260 | 8 | 194 | 5.28e-138 | 386 |
| MS.gene28934.t1 | MTR_4g098930 | 40.476 | 252 | 138 | 6 | 4 | 245 | 5 | 254 | 1.08e-62 | 197 |
| MS.gene28934.t1 | MTR_5g013440 | 38.934 | 244 | 139 | 5 | 4 | 242 | 12 | 250 | 8.38e-56 | 179 |
| MS.gene28934.t1 | MTR_4g099370 | 37.387 | 222 | 135 | 3 | 26 | 244 | 31 | 251 | 5.63e-53 | 172 |
| MS.gene28934.t1 | MTR_4g099400 | 35.882 | 170 | 108 | 1 | 74 | 242 | 9 | 178 | 1.83e-38 | 133 |
| MS.gene28934.t1 | MTR_0809s0010 | 32.864 | 213 | 138 | 4 | 26 | 234 | 50 | 261 | 5.13e-35 | 126 |
| MS.gene28934.t1 | MTR_2g090265 | 30.830 | 253 | 160 | 7 | 3 | 244 | 9 | 257 | 1.54e-29 | 112 |
| MS.gene28934.t1 | MTR_2g090270 | 30.078 | 256 | 162 | 7 | 2 | 244 | 8 | 259 | 4.56e-29 | 110 |
| MS.gene28934.t1 | MTR_0118s0070 | 31.579 | 209 | 136 | 4 | 40 | 242 | 67 | 274 | 1.03e-27 | 107 |
| MS.gene28934.t1 | MTR_0122s0020 | 29.389 | 262 | 160 | 7 | 2 | 242 | 6 | 263 | 3.49e-27 | 106 |
| MS.gene28934.t1 | MTR_4g028640 | 30.660 | 212 | 137 | 6 | 40 | 246 | 61 | 267 | 2.28e-26 | 103 |
| MS.gene28934.t1 | MTR_7g013120 | 30.556 | 252 | 144 | 9 | 5 | 238 | 10 | 248 | 2.07e-21 | 90.5 |
| MS.gene28934.t1 | MTR_7g013140 | 29.921 | 254 | 145 | 10 | 5 | 240 | 10 | 248 | 9.53e-21 | 88.6 |
| MS.gene28934.t1 | MTR_0122s0020 | 29.648 | 199 | 119 | 5 | 2 | 180 | 6 | 203 | 3.08e-20 | 86.3 |
| MS.gene28934.t1 | MTR_8g058990 | 29.845 | 258 | 148 | 9 | 5 | 243 | 10 | 253 | 9.78e-20 | 85.9 |
| MS.gene28934.t1 | MTR_8g464350 | 28.346 | 254 | 149 | 9 | 4 | 239 | 12 | 250 | 9.85e-20 | 85.9 |
| MS.gene28934.t1 | MTR_3g435430 | 29.675 | 246 | 138 | 10 | 11 | 237 | 13 | 242 | 1.15e-19 | 85.5 |
| MS.gene28934.t1 | MTR_4g124340 | 27.706 | 231 | 135 | 7 | 25 | 237 | 63 | 279 | 3.67e-19 | 84.7 |
| MS.gene28934.t1 | MTR_7g110990 | 30.242 | 248 | 133 | 10 | 19 | 242 | 7 | 238 | 3.78e-19 | 84.0 |
| MS.gene28934.t1 | MTR_7g013150 | 30.196 | 255 | 143 | 10 | 5 | 239 | 10 | 249 | 4.61e-19 | 84.0 |
| MS.gene28934.t1 | MTR_1g085460 | 29.752 | 242 | 131 | 10 | 24 | 242 | 31 | 256 | 6.09e-19 | 84.0 |
| MS.gene28934.t1 | MTR_7g111010 | 30.603 | 232 | 124 | 9 | 32 | 242 | 38 | 253 | 2.53e-18 | 82.0 |
| MS.gene28934.t1 | MTR_5g041700 | 29.457 | 258 | 145 | 11 | 1 | 237 | 1 | 242 | 5.15e-17 | 78.6 |
| MS.gene28934.t1 | MTR_5g011210 | 29.515 | 227 | 129 | 6 | 34 | 243 | 50 | 262 | 2.33e-16 | 77.0 |
| MS.gene28934.t1 | MTR_5g075320 | 30.909 | 220 | 121 | 9 | 34 | 237 | 42 | 246 | 7.95e-16 | 75.1 |
| MS.gene28934.t1 | MTR_4g102450 | 27.876 | 226 | 132 | 7 | 34 | 242 | 49 | 260 | 8.96e-16 | 75.1 |
| MS.gene28934.t1 | MTR_3g094040 | 28.054 | 221 | 126 | 9 | 34 | 237 | 44 | 248 | 2.91e-15 | 73.6 |
| MS.gene28934.t1 | MTR_3g094060 | 28.054 | 221 | 126 | 9 | 34 | 237 | 44 | 248 | 3.38e-15 | 73.6 |
| MS.gene28934.t1 | MTR_4g081950 | 27.928 | 222 | 129 | 7 | 32 | 237 | 72 | 278 | 9.74e-15 | 72.8 |
| MS.gene28934.t1 | MTR_7g061720 | 28.000 | 225 | 127 | 9 | 32 | 237 | 43 | 251 | 1.85e-14 | 71.6 |
| MS.gene28934.t1 | MTR_3g466760 | 26.126 | 222 | 129 | 9 | 34 | 237 | 51 | 255 | 1.90e-14 | 71.6 |
| MS.gene28934.t1 | MTR_1g026020 | 27.111 | 225 | 142 | 9 | 32 | 243 | 40 | 255 | 3.43e-14 | 70.9 |
| MS.gene28934.t1 | MTR_5g079950 | 28.000 | 225 | 127 | 10 | 32 | 237 | 44 | 252 | 5.04e-14 | 70.1 |
| MS.gene28934.t1 | MTR_0040s0140 | 26.587 | 252 | 148 | 11 | 4 | 234 | 13 | 248 | 1.18e-13 | 69.3 |
| MS.gene28934.t1 | MTR_2g090255 | 27.556 | 225 | 126 | 12 | 34 | 238 | 41 | 248 | 1.44e-13 | 68.9 |
| MS.gene28934.t1 | MTR_7g011060 | 25.794 | 252 | 152 | 10 | 1 | 237 | 4 | 235 | 1.48e-13 | 68.6 |
| MS.gene28934.t1 | MTR_3g466790 | 25.676 | 222 | 130 | 9 | 34 | 237 | 45 | 249 | 3.21e-13 | 67.8 |
| MS.gene28934.t1 | MTR_5g076950 | 26.293 | 232 | 137 | 9 | 26 | 238 | 44 | 260 | 2.22e-12 | 65.9 |
| MS.gene28934.t1 | MTR_2g097030 | 27.111 | 225 | 129 | 9 | 32 | 237 | 45 | 253 | 2.83e-12 | 65.5 |
| MS.gene28934.t1 | MTR_4g063975 | 28.723 | 188 | 103 | 7 | 32 | 201 | 43 | 217 | 1.10e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28934.t1 | AT3G45970 | 62.069 | 261 | 96 | 2 | 3 | 260 | 5 | 265 | 9.57e-112 | 322 |
| MS.gene28934.t1 | AT3G45960 | 64.173 | 254 | 89 | 1 | 3 | 254 | 5 | 258 | 3.74e-111 | 321 |
| MS.gene28934.t1 | AT4G38400 | 64.372 | 247 | 86 | 1 | 16 | 260 | 19 | 265 | 6.24e-110 | 318 |
| MS.gene28934.t1 | AT3G45970 | 60.793 | 227 | 86 | 2 | 37 | 260 | 12 | 238 | 2.69e-106 | 308 |
| MS.gene28934.t1 | AT3G45960 | 63.810 | 210 | 74 | 1 | 47 | 254 | 1 | 210 | 1.26e-101 | 295 |
| MS.gene28934.t1 | AT4G17030 | 39.640 | 222 | 132 | 1 | 25 | 244 | 29 | 250 | 1.09e-55 | 179 |
| MS.gene28934.t1 | AT1G65680 | 33.610 | 241 | 148 | 7 | 11 | 242 | 35 | 272 | 4.76e-31 | 116 |
| MS.gene28934.t1 | AT2G20750 | 31.698 | 265 | 153 | 9 | 5 | 246 | 11 | 270 | 6.95e-30 | 113 |
| MS.gene28934.t1 | AT4G28250 | 28.736 | 261 | 167 | 7 | 1 | 246 | 7 | 263 | 4.06e-28 | 108 |
| MS.gene28934.t1 | AT3G60570 | 31.925 | 213 | 134 | 8 | 38 | 244 | 3 | 210 | 1.90e-27 | 105 |
| MS.gene28934.t1 | AT3G60570 | 32.227 | 211 | 132 | 8 | 38 | 242 | 51 | 256 | 4.46e-27 | 105 |
| MS.gene28934.t1 | AT1G65681 | 28.571 | 210 | 142 | 4 | 38 | 242 | 15 | 221 | 9.18e-26 | 101 |
| MS.gene28934.t1 | AT2G45110 | 29.858 | 211 | 140 | 4 | 38 | 242 | 50 | 258 | 2.16e-24 | 98.6 |
| MS.gene28934.t1 | AT4G28250 | 27.969 | 261 | 163 | 8 | 1 | 246 | 7 | 257 | 3.29e-23 | 95.5 |
| MS.gene28934.t1 | AT2G20750 | 33.838 | 198 | 106 | 7 | 5 | 180 | 11 | 205 | 1.96e-22 | 92.4 |
| MS.gene28934.t1 | AT5G39290 | 28.340 | 247 | 146 | 11 | 12 | 242 | 47 | 278 | 1.03e-18 | 83.6 |
| MS.gene28934.t1 | AT1G20190 | 31.646 | 237 | 136 | 11 | 23 | 243 | 26 | 252 | 6.14e-18 | 80.9 |
| MS.gene28934.t1 | AT1G12560 | 32.057 | 209 | 126 | 8 | 40 | 239 | 56 | 257 | 7.99e-18 | 80.9 |
| MS.gene28934.t1 | AT5G39270 | 27.530 | 247 | 148 | 11 | 12 | 242 | 47 | 278 | 8.30e-17 | 78.6 |
| MS.gene28934.t1 | AT2G03090 | 28.000 | 250 | 145 | 10 | 7 | 237 | 14 | 247 | 8.78e-17 | 77.8 |
| MS.gene28934.t1 | AT5G05290 | 26.984 | 252 | 152 | 8 | 5 | 238 | 14 | 251 | 2.54e-16 | 76.6 |
| MS.gene28934.t1 | AT4G28250 | 33.562 | 146 | 84 | 5 | 40 | 180 | 55 | 192 | 2.94e-16 | 75.5 |
| MS.gene28934.t1 | AT2G40610 | 28.959 | 221 | 125 | 9 | 34 | 237 | 43 | 248 | 3.58e-16 | 76.3 |
| MS.gene28934.t1 | AT5G39260 | 27.706 | 231 | 154 | 7 | 13 | 238 | 35 | 257 | 3.60e-16 | 76.3 |
| MS.gene28934.t1 | AT5G39310 | 29.358 | 218 | 124 | 9 | 40 | 242 | 109 | 311 | 4.76e-16 | 76.6 |
| MS.gene28934.t1 | AT1G69530 | 29.412 | 221 | 123 | 8 | 34 | 237 | 40 | 244 | 5.67e-16 | 75.5 |
| MS.gene28934.t1 | AT1G69530 | 29.412 | 221 | 123 | 8 | 34 | 237 | 40 | 244 | 5.67e-16 | 75.5 |
| MS.gene28934.t1 | AT1G69530 | 29.018 | 224 | 126 | 8 | 34 | 240 | 40 | 247 | 8.88e-16 | 75.5 |
| MS.gene28934.t1 | AT4G28250 | 28.497 | 193 | 116 | 6 | 1 | 179 | 7 | 191 | 1.20e-15 | 73.9 |
| MS.gene28934.t1 | AT2G39700 | 28.889 | 225 | 125 | 9 | 32 | 237 | 42 | 250 | 1.48e-15 | 74.7 |
| MS.gene28934.t1 | AT5G02260 | 28.444 | 225 | 126 | 10 | 32 | 237 | 43 | 251 | 2.61e-15 | 73.9 |
| MS.gene28934.t1 | AT1G26770 | 26.087 | 253 | 152 | 8 | 1 | 234 | 4 | 240 | 3.85e-15 | 73.2 |
| MS.gene28934.t1 | AT1G26770 | 25.692 | 253 | 153 | 7 | 1 | 234 | 14 | 250 | 4.18e-15 | 73.2 |
| MS.gene28934.t1 | AT4G01630 | 26.692 | 266 | 153 | 11 | 1 | 243 | 9 | 255 | 8.13e-15 | 72.4 |
| MS.gene28934.t1 | AT5G56320 | 26.724 | 232 | 135 | 10 | 25 | 237 | 31 | 246 | 1.47e-14 | 71.6 |
| MS.gene28934.t1 | AT5G56320 | 26.724 | 232 | 135 | 10 | 25 | 237 | 31 | 246 | 1.47e-14 | 71.6 |
| MS.gene28934.t1 | AT5G56320 | 26.724 | 232 | 135 | 10 | 25 | 237 | 31 | 246 | 1.64e-14 | 72.4 |
| MS.gene28934.t1 | AT2G37640 | 27.982 | 218 | 136 | 7 | 32 | 237 | 47 | 255 | 6.59e-14 | 70.1 |
| MS.gene28934.t1 | AT1G69530 | 30.939 | 181 | 96 | 6 | 34 | 198 | 40 | 207 | 8.02e-14 | 68.9 |
| MS.gene28934.t1 | AT1G69530 | 30.939 | 181 | 96 | 6 | 34 | 198 | 40 | 207 | 1.06e-13 | 69.3 |
| MS.gene28934.t1 | AT5G39280 | 26.230 | 244 | 149 | 9 | 15 | 242 | 46 | 274 | 1.76e-13 | 68.9 |
| MS.gene28934.t1 | AT3G15370 | 29.339 | 242 | 140 | 10 | 15 | 239 | 19 | 246 | 2.72e-13 | 68.2 |
| MS.gene28934.t1 | AT2G37640 | 27.700 | 213 | 133 | 7 | 37 | 237 | 27 | 230 | 3.63e-13 | 67.4 |
| MS.gene28934.t1 | AT1G20190 | 31.000 | 200 | 114 | 10 | 57 | 242 | 3 | 192 | 4.42e-13 | 66.6 |
| MS.gene28934.t1 | AT5G39300 | 25.506 | 247 | 153 | 9 | 12 | 242 | 44 | 275 | 7.67e-13 | 67.0 |
| MS.gene28934.t1 | AT3G15370 | 30.698 | 215 | 120 | 9 | 40 | 239 | 76 | 276 | 1.74e-12 | 66.2 |
| MS.gene28934.t1 | AT4G38210 | 28.378 | 222 | 126 | 8 | 34 | 237 | 43 | 249 | 2.12e-12 | 65.9 |
| MS.gene28934.t1 | AT1G62980 | 28.829 | 222 | 127 | 8 | 35 | 239 | 45 | 252 | 3.17e-12 | 65.1 |
| MS.gene28934.t1 | AT2G28950 | 28.444 | 225 | 126 | 10 | 32 | 237 | 42 | 250 | 7.24e-12 | 64.3 |
| MS.gene28934.t1 | AT3G55500 | 26.638 | 229 | 125 | 10 | 32 | 237 | 45 | 253 | 1.30e-11 | 63.5 |
| MS.gene28934.t1 | AT3G29030 | 28.169 | 213 | 129 | 7 | 34 | 237 | 51 | 248 | 1.54e-11 | 63.2 |
| MS.gene28934.t1 | AT3G03220 | 24.786 | 234 | 142 | 9 | 24 | 238 | 41 | 259 | 4.39e-11 | 62.0 |
Find 51 sgRNAs with CRISPR-Local
Find 109 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGTTGTGGTGCTTGCTTTC+AGG | 0.151650 | 4.4:-34464174 | MS.gene28934:intron |
| GTTTCTTCACTCTTCTATAA+TGG | 0.287049 | 4.4:-34464200 | MS.gene28934:CDS |
| ACTAGGTTGGATCTTCAAAT+TGG | 0.295466 | 4.4:-34463010 | MS.gene28934:intron |
| AATTTAATTGCTAAATAATC+TGG | 0.300940 | 4.4:+34463196 | None:intergenic |
| CATCAGGGTATGATGGTAAA+TGG | 0.338953 | 4.4:-34462905 | MS.gene28934:CDS |
| GGCATTGCAATTTCGTTTAG+TGG | 0.366207 | 4.4:-34462931 | MS.gene28934:CDS |
| AAAAGGTTCTTCCAGCTGAT+TGG | 0.372823 | 4.4:-34462872 | MS.gene28934:CDS |
| TCTTTAGCACTTGACTTTAG+TGG | 0.377568 | 4.4:-34464242 | MS.gene28934:CDS |
| TTTGGAGAAATAAGAAGCTT+TGG | 0.378209 | 4.4:+34464391 | None:intergenic |
| TTTCGTTTAGTGGTTACATC+AGG | 0.387420 | 4.4:-34462921 | MS.gene28934:CDS |
| CTTTACAACTATGGCTCAAA+AGG | 0.399414 | 4.4:-34463822 | MS.gene28934:CDS |
| TTCTATAATGGAGCTGGTTG+TGG | 0.406537 | 4.4:-34464188 | MS.gene28934:CDS |
| TAGTAGAGCCTTTACAACTA+TGG | 0.427444 | 4.4:-34463831 | MS.gene28934:CDS |
| GCAGCTGGGGCATGTGGGTA+TGG | 0.430576 | 4.4:-34464266 | MS.gene28934:intron |
| TCACTCTTCTATAATGGAGC+TGG | 0.434156 | 4.4:-34464194 | MS.gene28934:CDS |
| ACATGAAAGAGCAGAAGCTT+TGG | 0.441652 | 4.4:+34464373 | None:intergenic |
| TTGTAAAGGCTCTACTACTT+AGG | 0.454795 | 4.4:+34463837 | None:intergenic |
| AGTGGTGGACACCTTGCAGC+TGG | 0.482221 | 4.4:-34464224 | MS.gene28934:CDS |
| TTATATGCAGCTGGGGCATG+TGG | 0.485952 | 4.4:-34464272 | MS.gene28934:intron |
| GGTATGATGGTAAATGGCTA+TGG | 0.487295 | 4.4:-34462899 | MS.gene28934:CDS |
| GCATTGTTGACATTGAATAC+AGG | 0.487778 | 4.4:-34463772 | MS.gene28934:CDS |
| AGTTTCTTGAGCAGAAATCA+TGG | 0.495684 | 4.4:-34462987 | MS.gene28934:CDS |
| GTGGTTACATCAGGGTATGA+TGG | 0.498564 | 4.4:-34462912 | MS.gene28934:CDS |
| GGGCAGTGTGGGACACAAGT+AGG | 0.499790 | 4.4:-34462965 | MS.gene28934:CDS |
| AAGATCAGTTAATACCACTT+TGG | 0.509266 | 4.4:+34463886 | None:intergenic |
| GACCATGTTTCATCATTACA+TGG | 0.510819 | 4.4:+34462786 | None:intergenic |
| GTTTCTTGAGCAGAAATCAT+GGG | 0.515717 | 4.4:-34462986 | MS.gene28934:CDS |
| AGTAGGTGTTGATGTAGCTC+AGG | 0.516706 | 4.4:-34463146 | MS.gene28934:intron |
| CAGAAATCATGGGGCAGTGT+GGG | 0.519402 | 4.4:-34462976 | MS.gene28934:CDS |
| CAAGCAATATGCACAAAAGA+AGG | 0.520775 | 4.4:-34463911 | MS.gene28934:CDS |
| TGCCAAGTTTCAAAATCTGT+TGG | 0.529827 | 4.4:+34463792 | None:intergenic |
| TATATGCAGCTGGGGCATGT+GGG | 0.536289 | 4.4:-34464271 | MS.gene28934:intron |
| GGCAGTGTGGGACACAAGTA+GGG | 0.554581 | 4.4:-34462964 | MS.gene28934:CDS |
| TTAGCACTTGACTTTAGTGG+TGG | 0.565026 | 4.4:-34464239 | MS.gene28934:CDS |
| TTCGTTTAGTGGTTACATCA+GGG | 0.571759 | 4.4:-34462920 | MS.gene28934:CDS |
| TTTACAACTATGGCTCAAAA+GGG | 0.578479 | 4.4:-34463821 | MS.gene28934:CDS |
| ACACAAGTAGGGTTCCACAT+GGG | 0.581518 | 4.4:-34462953 | MS.gene28934:CDS |
| GATTACAGAAATTGCACAGG+AGG | 0.581799 | 4.4:-34462817 | MS.gene28934:CDS |
| CACAAGTAGGGTTCCACATG+GGG | 0.582018 | 4.4:-34462952 | MS.gene28934:CDS |
| TCAGATTACAGAAATTGCAC+AGG | 0.586054 | 4.4:-34462820 | MS.gene28934:CDS |
| GTATGATGGTAAATGGCTAT+GGG | 0.593985 | 4.4:-34462898 | MS.gene28934:CDS |
| GTGAAGAAACACCAGCTGCA+AGG | 0.611075 | 4.4:+34464213 | None:intergenic |
| CAAGCAACGATCACAAGCAG+TGG | 0.634417 | 4.4:+34464421 | None:intergenic |
| TTGTTGACATTGAATACAGG+AGG | 0.635488 | 4.4:-34463769 | MS.gene28934:intron |
| GCAGAAATCATGGGGCAGTG+TGG | 0.638652 | 4.4:-34462977 | MS.gene28934:CDS |
| ATTACAGAAATTGCACAGGA+GGG | 0.647308 | 4.4:-34462816 | MS.gene28934:CDS |
| CGAAATTGCAATGCCCCATG+TGG | 0.649143 | 4.4:+34462939 | None:intergenic |
| GACACAAGTAGGGTTCCACA+TGG | 0.649693 | 4.4:-34462954 | MS.gene28934:CDS |
| TTTCTTGAGCAGAAATCATG+GGG | 0.654832 | 4.4:-34462985 | MS.gene28934:CDS |
| CACAAAAGAAGGTACCAAAG+TGG | 0.665562 | 4.4:-34463900 | MS.gene28934:CDS |
| CACCATGTAATGATGAAACA+TGG | 0.686342 | 4.4:-34462788 | MS.gene28934:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAATATATTTATTTAATT+TGG | + | chr4.4:34463250-34463269 | None:intergenic | 0.0% |
| !! | TTAAAAAAATAATTAACCAT+TGG | - | chr4.4:34464194-34464213 | MS.gene28934:CDS | 10.0% |
| !! | TTTATTTATCAATTTGTATA+TGG | - | chr4.4:34463926-34463945 | MS.gene28934:CDS | 10.0% |
| !! | AATTTAATTGCTAAATAATC+TGG | + | chr4.4:34464056-34464075 | None:intergenic | 15.0% |
| !!! | AATTAAATTTTTGTATCAAG+GGG | - | chr4.4:34464067-34464086 | MS.gene28934:intron | 15.0% |
| !!! | ATTTTATAATGTCGTAAATT+TGG | - | chr4.4:34463870-34463889 | MS.gene28934:CDS | 15.0% |
| !!! | CAATTAAATTTTTGTATCAA+GGG | - | chr4.4:34464066-34464085 | MS.gene28934:intron | 15.0% |
| !! | TTATTGATGTCATGAAAATT+TGG | - | chr4.4:34463268-34463287 | MS.gene28934:intron | 20.0% |
| !! | TTTAATTCAATAGAAACACT+TGG | + | chr4.4:34464179-34464198 | None:intergenic | 20.0% |
| !!! | ATTAAATTTTTGTATCAAGG+GGG | - | chr4.4:34464068-34464087 | MS.gene28934:intron | 20.0% |
| !!! | ATTTAATTTGGCTAAATGTT+AGG | + | chr4.4:34463238-34463257 | None:intergenic | 20.0% |
| !!! | GCAATTAAATTTTTGTATCA+AGG | - | chr4.4:34464065-34464084 | MS.gene28934:intron | 20.0% |
| !!! | TTCATATAATTTGTCTACAT+TGG | + | chr4.4:34463702-34463721 | None:intergenic | 20.0% |
| ! | AAAGTAAAGCTACAAAAAAG+TGG | + | chr4.4:34463519-34463538 | None:intergenic | 25.0% |
| ! | ATTAATGAACAATTTGATGC+AGG | - | chr4.4:34463301-34463320 | MS.gene28934:intron | 25.0% |
| ! | TAATGTCGTAAATTTGGAAA+TGG | - | chr4.4:34463876-34463895 | MS.gene28934:CDS | 25.0% |
| ! | TAGCGCAATAATAATAGTAA+CGG | - | chr4.4:34463738-34463757 | MS.gene28934:intron | 25.0% |
| ! | TGAGTACAAAAAACAGAATT+TGG | - | chr4.4:34464001-34464020 | MS.gene28934:intron | 25.0% |
| !!! | ATTTTTTTTATATGCAGCTG+GGG | - | chr4.4:34462970-34462989 | MS.gene28934:CDS | 25.0% |
| !!! | CTTTTTAATTACTACTAGGT+TGG | - | chr4.4:34464226-34464245 | MS.gene28934:CDS | 25.0% |
| !!! | GATTTTTTTTATATGCAGCT+GGG | - | chr4.4:34462969-34462988 | MS.gene28934:CDS | 25.0% |
| !!! | GTTAGGATAGTTTTAAAATG+CGG | + | chr4.4:34463221-34463240 | None:intergenic | 25.0% |
| !!! | TGATTTTTTTTATATGCAGC+TGG | - | chr4.4:34462968-34462987 | MS.gene28934:CDS | 25.0% |
| AAGATCAGTTAATACCACTT+TGG | + | chr4.4:34463366-34463385 | None:intergenic | 30.0% | |
| CATGATTAATAACACTGCAA+CGG | + | chr4.4:34463152-34463171 | None:intergenic | 30.0% | |
| GTTTCTTCACTCTTCTATAA+TGG | - | chr4.4:34463049-34463068 | MS.gene28934:intron | 30.0% | |
| TTTACAACTATGGCTCAAAA+GGG | - | chr4.4:34463428-34463447 | MS.gene28934:intron | 30.0% | |
| TTTGGAGAAATAAGAAGCTT+TGG | + | chr4.4:34462861-34462880 | None:intergenic | 30.0% | |
| !! | CATTGGTGTTCTATTTTCAA+TGG | + | chr4.4:34463685-34463704 | None:intergenic | 30.0% |
| !! | GTGGCTTTTTAATTACTACT+AGG | - | chr4.4:34464222-34464241 | MS.gene28934:CDS | 30.0% |
| !! | TAATTAACCATTGGTTTCTG+TGG | - | chr4.4:34464203-34464222 | MS.gene28934:CDS | 30.0% |
| !!! | CACTTGGTAATGATTTTTGT+TGG | + | chr4.4:34464163-34464182 | None:intergenic | 30.0% |
| !!! | TTCTGTTTTTTGTACTCACA+TGG | + | chr4.4:34463999-34464018 | None:intergenic | 30.0% |
| AAAACCCGAATACGATGATT+TGG | + | chr4.4:34463108-34463127 | None:intergenic | 35.0% | |
| AAACCCGAATACGATGATTT+GGG | + | chr4.4:34463107-34463126 | None:intergenic | 35.0% | |
| AATATGCCAGCATTTCTTGT+TGG | - | chr4.4:34463618-34463637 | MS.gene28934:intron | 35.0% | |
| AGTTTCTTGAGCAGAAATCA+TGG | - | chr4.4:34464262-34464281 | MS.gene28934:CDS | 35.0% | |
| ATTACAGAAATTGCACAGGA+GGG | - | chr4.4:34464433-34464452 | MS.gene28934:CDS | 35.0% | |
| CAAGCAATATGCACAAAAGA+AGG | - | chr4.4:34463338-34463357 | MS.gene28934:intron | 35.0% | |
| CACCATGTAATGATGAAACA+TGG | - | chr4.4:34464461-34464480 | MS.gene28934:CDS | 35.0% | |
| CTGGCATATTATCAAGAATG+TGG | + | chr4.4:34463608-34463627 | None:intergenic | 35.0% | |
| CTTTACAACTATGGCTCAAA+AGG | - | chr4.4:34463427-34463446 | MS.gene28934:intron | 35.0% | |
| GACCATGTTTCATCATTACA+TGG | + | chr4.4:34464466-34464485 | None:intergenic | 35.0% | |
| GCATTGTTGACATTGAATAC+AGG | - | chr4.4:34463477-34463496 | MS.gene28934:intron | 35.0% | |
| GTATGATGGTAAATGGCTAT+GGG | - | chr4.4:34464351-34464370 | MS.gene28934:intron | 35.0% | |
| GTTTCTTGAGCAGAAATCAT+GGG | - | chr4.4:34464263-34464282 | MS.gene28934:CDS | 35.0% | |
| TAAAAAGCCACAGAAACCAA+TGG | + | chr4.4:34464213-34464232 | None:intergenic | 35.0% | |
| TAAATGGCTATGGGCAAAAA+AGG | - | chr4.4:34464360-34464379 | MS.gene28934:intron | 35.0% | |
| TAGTAGAGCCTTTACAACTA+TGG | - | chr4.4:34463418-34463437 | MS.gene28934:intron | 35.0% | |
| TCAGATTACAGAAATTGCAC+AGG | - | chr4.4:34464429-34464448 | MS.gene28934:CDS | 35.0% | |
| TCTTTAGCACTTGACTTTAG+TGG | - | chr4.4:34463007-34463026 | MS.gene28934:CDS | 35.0% | |
| TGCCAAGTTTCAAAATCTGT+TGG | + | chr4.4:34463460-34463479 | None:intergenic | 35.0% | |
| TGTACCCAAATCATCGTATT+CGG | - | chr4.4:34463100-34463119 | MS.gene28934:intron | 35.0% | |
| TTCCAGCTGATTGGAAAAAT+GGG | - | chr4.4:34464386-34464405 | MS.gene28934:CDS | 35.0% | |
| TTCGTTTAGTGGTTACATCA+GGG | - | chr4.4:34464329-34464348 | MS.gene28934:intron | 35.0% | |
| TTGTAAAGGCTCTACTACTT+AGG | + | chr4.4:34463415-34463434 | None:intergenic | 35.0% | |
| TTGTTGACATTGAATACAGG+AGG | - | chr4.4:34463480-34463499 | MS.gene28934:intron | 35.0% | |
| TTTCGTTTAGTGGTTACATC+AGG | - | chr4.4:34464328-34464347 | MS.gene28934:intron | 35.0% | |
| TTTCTTGAGCAGAAATCATG+GGG | - | chr4.4:34464264-34464283 | MS.gene28934:CDS | 35.0% | |
| ! | ACTAGGTTGGATCTTCAAAT+TGG | - | chr4.4:34464239-34464258 | MS.gene28934:CDS | 35.0% |
| ! | CTTCTTTTGTGCATATTGCT+TGG | + | chr4.4:34463340-34463359 | None:intergenic | 35.0% |
| ! | CTTTTGAGCCATAGTTGTAA+AGG | + | chr4.4:34463429-34463448 | None:intergenic | 35.0% |
| ! | GGTGTTCTATTTTCAATGGT+GGG | + | chr4.4:34463681-34463700 | None:intergenic | 35.0% |
| ! | TAATCCCACGTGGATTTTAA+TGG | - | chr4.4:34463964-34463983 | MS.gene28934:intron | 35.0% |
| ! | TGGTGTTCTATTTTCAATGG+TGG | + | chr4.4:34463682-34463701 | None:intergenic | 35.0% |
| !! | TTCTATTTTCAATGGTGGGA+CGG | + | chr4.4:34463677-34463696 | None:intergenic | 35.0% |
| !!! | AGCCAACAGATTTTGAAACT+TGG | - | chr4.4:34463455-34463474 | MS.gene28934:intron | 35.0% |
| AAAAGGTTCTTCCAGCTGAT+TGG | - | chr4.4:34464377-34464396 | MS.gene28934:CDS | 40.0% | |
| ACATGAAAGAGCAGAAGCTT+TGG | + | chr4.4:34462879-34462898 | None:intergenic | 40.0% | |
| CATCAGGGTATGATGGTAAA+TGG | - | chr4.4:34464344-34464363 | MS.gene28934:intron | 40.0% | |
| CTTCCAGCTGATTGGAAAAA+TGG | - | chr4.4:34464385-34464404 | MS.gene28934:CDS | 40.0% | |
| GATTACAGAAATTGCACAGG+AGG | - | chr4.4:34464432-34464451 | MS.gene28934:CDS | 40.0% | |
| GGCATTGCAATTTCGTTTAG+TGG | - | chr4.4:34464318-34464337 | MS.gene28934:intron | 40.0% | |
| GGTATGATGGTAAATGGCTA+TGG | - | chr4.4:34464350-34464369 | MS.gene28934:intron | 40.0% | |
| GTACCCAAATCATCGTATTC+GGG | - | chr4.4:34463101-34463120 | MS.gene28934:intron | 40.0% | |
| TCCAGCTGATTGGAAAAATG+GGG | - | chr4.4:34464387-34464406 | MS.gene28934:CDS | 40.0% | |
| TGCACCATTAAAATCCACGT+GGG | + | chr4.4:34463971-34463990 | None:intergenic | 40.0% | |
| TTAGCACTTGACTTTAGTGG+TGG | - | chr4.4:34463010-34463029 | MS.gene28934:intron | 40.0% | |
| ! | GAACATTGATTAATCCCACG+TGG | - | chr4.4:34463954-34463973 | MS.gene28934:intron | 40.0% |
| ! | TCACTCTTCTATAATGGAGC+TGG | - | chr4.4:34463055-34463074 | MS.gene28934:intron | 40.0% |
| ! | TTCTATAATGGAGCTGGTTG+TGG | - | chr4.4:34463061-34463080 | MS.gene28934:intron | 40.0% |
| !! | CACAAAAGAAGGTACCAAAG+TGG | - | chr4.4:34463349-34463368 | MS.gene28934:intron | 40.0% |
| ACACAAGTAGGGTTCCACAT+GGG | - | chr4.4:34464296-34464315 | MS.gene28934:intron | 45.0% | |
| CTGCACCATTAAAATCCACG+TGG | + | chr4.4:34463972-34463991 | None:intergenic | 45.0% | |
| GTGGTTACATCAGGGTATGA+TGG | - | chr4.4:34464337-34464356 | MS.gene28934:intron | 45.0% | |
| !! | ACCCCATTTTTCCAATCAGC+TGG | + | chr4.4:34464391-34464410 | None:intergenic | 45.0% |
| !! | AGTAGGTGTTGATGTAGCTC+AGG | - | chr4.4:34464103-34464122 | MS.gene28934:intron | 45.0% |
| CAAGCAACGATCACAAGCAG+TGG | + | chr4.4:34462831-34462850 | None:intergenic | 50.0% | |
| CACAAGTAGGGTTCCACATG+GGG | - | chr4.4:34464297-34464316 | MS.gene28934:intron | 50.0% | |
| CAGAAATCATGGGGCAGTGT+GGG | - | chr4.4:34464273-34464292 | MS.gene28934:intron | 50.0% | |
| CCACTGTCTACTAGCTAGGT+GGG | + | chr4.4:34463652-34463671 | None:intergenic | 50.0% | |
| CGAAATTGCAATGCCCCATG+TGG | + | chr4.4:34464313-34464332 | None:intergenic | 50.0% | |
| CTACTAGCTAGGTGGGAAGT+GGG | + | chr4.4:34463645-34463664 | None:intergenic | 50.0% | |
| GACACAAGTAGGGTTCCACA+TGG | - | chr4.4:34464295-34464314 | MS.gene28934:intron | 50.0% | |
| GGGGGTCAAACAGAGATAGT+AGG | - | chr4.4:34464086-34464105 | MS.gene28934:intron | 50.0% | |
| GTGAAGAAACACCAGCTGCA+AGG | + | chr4.4:34463039-34463058 | None:intergenic | 50.0% | |
| GTGGGTCCAACAAGAAATGC+TGG | + | chr4.4:34463627-34463646 | None:intergenic | 50.0% | |
| TATATGCAGCTGGGGCATGT+GGG | - | chr4.4:34462978-34462997 | MS.gene28934:CDS | 50.0% | |
| TCTACTAGCTAGGTGGGAAG+TGG | + | chr4.4:34463646-34463665 | None:intergenic | 50.0% | |
| TGGTTGTGGTGCTTGCTTTC+AGG | - | chr4.4:34463075-34463094 | MS.gene28934:intron | 50.0% | |
| TTATATGCAGCTGGGGCATG+TGG | - | chr4.4:34462977-34462996 | MS.gene28934:CDS | 50.0% | |
| CCCACCTAGCTAGTAGACAG+TGG | - | chr4.4:34463649-34463668 | MS.gene28934:intron | 55.0% | |
| GCAGAAATCATGGGGCAGTG+TGG | - | chr4.4:34464272-34464291 | MS.gene28934:intron | 55.0% | |
| GCCACTGTCTACTAGCTAGG+TGG | + | chr4.4:34463653-34463672 | None:intergenic | 55.0% | |
| GGCAGTGTGGGACACAAGTA+GGG | - | chr4.4:34464285-34464304 | MS.gene28934:intron | 55.0% | |
| !! | GGTGCCACTGTCTACTAGCT+AGG | + | chr4.4:34463656-34463675 | None:intergenic | 55.0% |
| GGGCAGTGTGGGACACAAGT+AGG | - | chr4.4:34464284-34464303 | MS.gene28934:intron | 60.0% | |
| !! | AGTGGTGGACACCTTGCAGC+TGG | - | chr4.4:34463025-34463044 | MS.gene28934:intron | 60.0% |
| GCAGCTGGGGCATGTGGGTA+TGG | - | chr4.4:34462983-34463002 | MS.gene28934:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 34462782 | 34464489 | 34462782 | ID=MS.gene28934 |
| chr4.4 | mRNA | 34462782 | 34464489 | 34462782 | ID=MS.gene28934.t1;Parent=MS.gene28934 |
| chr4.4 | exon | 34464375 | 34464489 | 34464375 | ID=MS.gene28934.t1.exon1;Parent=MS.gene28934.t1 |
| chr4.4 | CDS | 34464375 | 34464489 | 34464375 | ID=cds.MS.gene28934.t1;Parent=MS.gene28934.t1 |
| chr4.4 | exon | 34464175 | 34464284 | 34464175 | ID=MS.gene28934.t1.exon2;Parent=MS.gene28934.t1 |
| chr4.4 | CDS | 34464175 | 34464284 | 34464175 | ID=cds.MS.gene28934.t1;Parent=MS.gene28934.t1 |
| chr4.4 | exon | 34463770 | 34463948 | 34463770 | ID=MS.gene28934.t1.exon3;Parent=MS.gene28934.t1 |
| chr4.4 | CDS | 34463770 | 34463948 | 34463770 | ID=cds.MS.gene28934.t1;Parent=MS.gene28934.t1 |
| chr4.4 | exon | 34463147 | 34463279 | 34463147 | ID=MS.gene28934.t1.exon4;Parent=MS.gene28934.t1 |
| chr4.4 | CDS | 34463147 | 34463279 | 34463147 | ID=cds.MS.gene28934.t1;Parent=MS.gene28934.t1 |
| chr4.4 | exon | 34462782 | 34463027 | 34462782 | ID=MS.gene28934.t1.exon5;Parent=MS.gene28934.t1 |
| chr4.4 | CDS | 34462782 | 34463027 | 34462782 | ID=cds.MS.gene28934.t1;Parent=MS.gene28934.t1 |
| Gene Sequence |
| Protein sequence |
