AlfalfaGEDB | MS.gene91837

M. sativa cultivar XinJiangDaYe / MS.gene91837

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91837.t1	ABN09016.1	88.2	93	11	0	1	93	216	308	6.10E-41	176.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91837.t1	Q9MAA2	55.1	89	40	0	1	89	188	276	2.2e-23	109.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91837.t1	A2Q5Z6	88.2	93	11	0	1	93	216	308	4.4e-41	176.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052447	MS.gene91837	0.808798	2.60E-50	-1.69E-46
MS.gene055952	MS.gene91837	0.817838	2.68E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91837.t1	MTR_7g113920	88.172	93	11	0	1	93	216	308	1.10e-55	174
MS.gene91837.t1	MTR_1g082360	57.609	92	37	1	1	90	184	275	1.16e-32	114
MS.gene91837.t1	MTR_5g010090	52.055	73	35	0	1	73	262	334	4.63e-22	88.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91837.t1	AT3G05170	55.056	89	40	0	1	89	188	276	2.14e-28	104
MS.gene91837.t1	AT1G08940	47.191	89	46	1	2	89	192	280	4.89e-24	92.4

Find 25 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTGAACTTCTCAACAATTT+TGG	0.120449	3.2:+22454984	MS.gene91837:CDS
TTCTGATCAGCTACCATATC+AGG	0.328803	3.2:-22455104	None:intergenic
TCCCAATGAGGTGGTTTAGA+TGG	0.339042	3.2:+22454949	None:intergenic
GTCCATCTAAACCACCTCAT+TGG	0.396126	3.2:-22454951	None:intergenic
AGTTATGTGTGATGCAGTTT+GGG	0.413875	3.2:+22455015	MS.gene91837:CDS
TCAAGGGAGCCTTGCTGCCA+TGG	0.424486	3.2:-22455136	None:intergenic
GAGTTATGTGTGATGCAGTT+TGG	0.426507	3.2:+22455014	MS.gene91837:CDS
CAGACGAAGAACTGCTTAAG+TGG	0.455115	3.2:+22455072	MS.gene91837:CDS
TGAGATCAAGAAATGCTTCA+AGG	0.472093	3.2:-22455178	None:intergenic
GTGGGGACTTTGAACATTCA+AGG	0.472238	3.2:-22455153	None:intergenic
TGTGTGATGCAGTTTGGGAC+TGG	0.480347	3.2:+22455020	MS.gene91837:CDS
CAAGAAATGCTTCAAGGCAG+TGG	0.487042	3.2:-22455172	None:intergenic
AAGAAATGCTTCAAGGCAGT+GGG	0.528278	3.2:-22455171	None:intergenic
ATATGGTAGCTGATCAGAAA+TGG	0.538142	3.2:+22455108	MS.gene91837:CDS
CAAGGGAGCCTTGCTGCCAT+GGG	0.546859	3.2:-22455135	None:intergenic
TGGGGACTTTGAACATTCAA+GGG	0.579415	3.2:-22455152	None:intergenic
AGACGAAGAACTGCTTAAGT+GGG	0.581964	3.2:+22455073	MS.gene91837:CDS
GTGATGCAGTTTGGGACTGG+TGG	0.592908	3.2:+22455023	MS.gene91837:CDS
GACTGGTGGAGAGTACAGCT+TGG	0.620408	3.2:+22455037	MS.gene91837:CDS
GATCAGAAATGGTGTGCCCA+TGG	0.629856	3.2:+22455119	MS.gene91837:CDS
ATGGTGTGCCCATGGCAGCA+AGG	0.643428	3.2:+22455127	MS.gene91837:CDS
GACGAAGAACTGCTTAAGTG+GGG	0.644712	3.2:+22455074	MS.gene91837:CDS
TGATTCTGATGACGAGAGTG+TGG	0.652927	3.2:+22455205	MS.gene91837:CDS
ACGAAGAACTGCTTAAGTGG+GGG	0.680035	3.2:+22455075	MS.gene91837:CDS
AGAAATGCTTCAAGGCAGTG+GGG	0.713932	3.2:-22455170	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTTGAACTTCTCAACAATTT+TGG	+	chr3.2:22454984-22455003	MS.gene91837:CDS	25.0%
	AGTTATGTGTGATGCAGTTT+GGG	+	chr3.2:22455015-22455034	MS.gene91837:CDS	35.0%
	ATATGGTAGCTGATCAGAAA+TGG	+	chr3.2:22455108-22455127	MS.gene91837:CDS	35.0%
	TGAGATCAAGAAATGCTTCA+AGG	-	chr3.2:22455181-22455200	None:intergenic	35.0%
	AAGAAATGCTTCAAGGCAGT+GGG	-	chr3.2:22455174-22455193	None:intergenic	40.0%
	AGACGAAGAACTGCTTAAGT+GGG	+	chr3.2:22455073-22455092	MS.gene91837:CDS	40.0%
	GAGTTATGTGTGATGCAGTT+TGG	+	chr3.2:22455014-22455033	MS.gene91837:CDS	40.0%
	TGGGGACTTTGAACATTCAA+GGG	-	chr3.2:22455155-22455174	None:intergenic	40.0%
	TTCTGATCAGCTACCATATC+AGG	-	chr3.2:22455107-22455126	None:intergenic	40.0%
	ACGAAGAACTGCTTAAGTGG+GGG	+	chr3.2:22455075-22455094	MS.gene91837:CDS	45.0%
	AGAAATGCTTCAAGGCAGTG+GGG	-	chr3.2:22455173-22455192	None:intergenic	45.0%
	CAAGAAATGCTTCAAGGCAG+TGG	-	chr3.2:22455175-22455194	None:intergenic	45.0%
	CAGACGAAGAACTGCTTAAG+TGG	+	chr3.2:22455072-22455091	MS.gene91837:CDS	45.0%
	GACGAAGAACTGCTTAAGTG+GGG	+	chr3.2:22455074-22455093	MS.gene91837:CDS	45.0%
	GTGGGGACTTTGAACATTCA+AGG	-	chr3.2:22455156-22455175	None:intergenic	45.0%
!	TGATTCTGATGACGAGAGTG+TGG	+	chr3.2:22455205-22455224	MS.gene91837:CDS	45.0%
	GATCAGAAATGGTGTGCCCA+TGG	+	chr3.2:22455119-22455138	MS.gene91837:CDS	50.0%
!	TGTGTGATGCAGTTTGGGAC+TGG	+	chr3.2:22455020-22455039	MS.gene91837:CDS	50.0%
!	GACTGGTGGAGAGTACAGCT+TGG	+	chr3.2:22455037-22455056	MS.gene91837:CDS	55.0%
!	GTGATGCAGTTTGGGACTGG+TGG	+	chr3.2:22455023-22455042	MS.gene91837:CDS	55.0%
	ATGGTGTGCCCATGGCAGCA+AGG	+	chr3.2:22455127-22455146	MS.gene91837:CDS	60.0%
	CAAGGGAGCCTTGCTGCCAT+GGG	-	chr3.2:22455138-22455157	None:intergenic	60.0%
	GTGGGGGCTCTCTCCTGATA+TGG	+	chr3.2:22455091-22455110	MS.gene91837:CDS	60.0%
	TCAAGGGAGCCTTGCTGCCA+TGG	-	chr3.2:22455139-22455158	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	22454954	22455235	22454954	ID=MS.gene91837
chr3.2	mRNA	22454954	22455235	22454954	ID=MS.gene91837.t1;Parent=MS.gene91837
chr3.2	exon	22454954	22455235	22454954	ID=MS.gene91837.t1.exon1;Parent=MS.gene91837.t1
chr3.2	CDS	22454954	22455235	22454954	ID=cds.MS.gene91837.t1;Parent=MS.gene91837.t1

Gene Sequence	>MS.gene91837 ATGAGGTGGTTTAGATGGACAGTTGAACAATTTGAACTTCTCAACAATTTTGGAAACTATGAGTTATGTGTGATGCAGTTTGGGACTGGTGGAGAGTACAGCTTGGCAGTTCATCATACAGACGAAGAACTGCTTAAGTGGGGGCTCTCTCCTGATATGGTAGCTGATCAGAAATGGTGTGCCCATGGCAGCAAGGCTCCCTTGAATGTTCAAAGTCCCCACTGCCTTGAAGCATTTCTTGATCTCATTACTGATTCTGATGACGAGAGTGTGGACACATAA
Protein sequence	>MS.gene91837.t1 MRWFRWTVEQFELLNNFGNYELCVMQFGTGGEYSLAVHHTDEELLKWGLSPDMVADQKWCAHGSKAPLNVQSPHCLEAFLDLITDSDDESVDT