Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055952.t1 | PNX96577.1 | 70.6 | 160 | 38 | 3 | 3 | 154 | 9 | 167 | 2.90E-48 | 201.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055952.t1 | A0A2K3N0L9 | 70.6 | 160 | 38 | 3 | 3 | 154 | 9 | 167 | 2.1e-48 | 201.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052447 | MS.gene055952 | 0.82468 | 7.08E-54 | -1.69E-46 |
| MS.gene055952 | MS.gene060654 | 0.802023 | 6.83E-49 | -1.69E-46 |
| MS.gene055952 | MS.gene062815 | 0.807231 | 5.59E-50 | -1.69E-46 |
| MS.gene055952 | MS.gene064075 | 0.806024 | 1.01E-49 | -1.69E-46 |
| MS.gene055952 | MS.gene066313 | 0.808849 | 2.53E-50 | -1.69E-46 |
| MS.gene055952 | MS.gene06633 | 0.812283 | 4.58E-51 | -1.69E-46 |
| MS.gene055952 | MS.gene068494 | 0.828895 | 6.97E-55 | -1.69E-46 |
| MS.gene055952 | MS.gene069271 | 0.815417 | 9.34E-52 | -1.69E-46 |
| MS.gene055952 | MS.gene069275 | 0.810569 | 1.08E-50 | -1.69E-46 |
| MS.gene055952 | MS.gene069743 | 0.809776 | 1.60E-50 | -1.69E-46 |
| MS.gene055952 | MS.gene07342 | 0.808009 | 3.82E-50 | -1.69E-46 |
| MS.gene055952 | MS.gene20657 | 0.851115 | 1.10E-60 | -1.69E-46 |
| MS.gene055952 | MS.gene25358 | 0.802199 | 6.28E-49 | -1.69E-46 |
| MS.gene055952 | MS.gene29008 | 0.815029 | 1.14E-51 | -1.69E-46 |
| MS.gene055952 | MS.gene29654 | 0.80521 | 1.49E-49 | -1.69E-46 |
| MS.gene055952 | MS.gene30073 | 0.824652 | 7.19E-54 | -1.69E-46 |
| MS.gene055952 | MS.gene30580 | 0.827916 | 1.20E-54 | -1.69E-46 |
| MS.gene055952 | MS.gene34615 | 0.813029 | 3.15E-51 | -1.69E-46 |
| MS.gene055952 | MS.gene35160 | 0.813751 | 2.18E-51 | -1.69E-46 |
| MS.gene055952 | MS.gene38444 | 0.801686 | 8.01E-49 | -1.69E-46 |
| MS.gene055952 | MS.gene38659 | 0.823891 | 1.08E-53 | -1.69E-46 |
| MS.gene055952 | MS.gene38850 | 0.814694 | 1.35E-51 | -1.69E-46 |
| MS.gene055952 | MS.gene41990 | 0.824075 | 9.82E-54 | -1.69E-46 |
| MS.gene055952 | MS.gene42573 | 0.814525 | 1.47E-51 | -1.69E-46 |
| MS.gene055952 | MS.gene42887 | 0.839449 | 1.57E-57 | -1.69E-46 |
| MS.gene055952 | MS.gene42891 | 0.84032 | 9.34E-58 | -1.69E-46 |
| MS.gene055952 | MS.gene44574 | 0.853352 | 2.53E-61 | -1.69E-46 |
| MS.gene055952 | MS.gene44575 | 0.85368 | 2.04E-61 | -1.69E-46 |
| MS.gene055952 | MS.gene44576 | 0.852568 | 4.24E-61 | -1.69E-46 |
| MS.gene055952 | MS.gene47552 | 0.824126 | 9.55E-54 | -1.69E-46 |
| MS.gene055952 | MS.gene48858 | 0.826174 | 3.13E-54 | -1.69E-46 |
| MS.gene055952 | MS.gene49642 | 0.870035 | 1.98E-66 | -1.69E-46 |
| MS.gene055952 | MS.gene49643 | 0.850894 | 1.26E-60 | -1.69E-46 |
| MS.gene055952 | MS.gene49651 | 0.832528 | 8.98E-56 | -1.69E-46 |
| MS.gene055952 | MS.gene50323 | 0.81019 | 1.30E-50 | -1.69E-46 |
| MS.gene055952 | MS.gene51252 | 0.802804 | 4.71E-49 | -1.69E-46 |
| MS.gene055952 | MS.gene51597 | 0.811336 | 7.37E-51 | -1.69E-46 |
| MS.gene055952 | MS.gene52697 | 0.830981 | 2.16E-55 | -1.69E-46 |
| MS.gene055952 | MS.gene55257 | 0.810079 | 1.38E-50 | -1.69E-46 |
| MS.gene055952 | MS.gene59780 | 0.804223 | 2.40E-49 | -1.69E-46 |
| MS.gene055952 | MS.gene61551 | 0.811888 | 5.59E-51 | -1.69E-46 |
| MS.gene055952 | MS.gene62748 | 0.826887 | 2.12E-54 | -1.69E-46 |
| MS.gene055952 | MS.gene63141 | 0.811883 | 5.60E-51 | -1.69E-46 |
| MS.gene055952 | MS.gene63142 | 0.81874 | 1.67E-52 | -1.69E-46 |
| MS.gene055952 | MS.gene65003 | 0.808934 | 2.43E-50 | -1.69E-46 |
| MS.gene055952 | MS.gene66277 | 0.81197 | 5.36E-51 | -1.69E-46 |
| MS.gene055952 | MS.gene66936 | 0.80626 | 8.97E-50 | -1.69E-46 |
| MS.gene055952 | MS.gene73540 | 0.827997 | 1.15E-54 | -1.69E-46 |
| MS.gene055952 | MS.gene74641 | 0.805293 | 1.43E-49 | -1.69E-46 |
| MS.gene055952 | MS.gene76061 | 0.827527 | 1.49E-54 | -1.69E-46 |
| MS.gene055952 | MS.gene76612 | 0.817712 | 2.86E-52 | -1.69E-46 |
| MS.gene055952 | MS.gene78453 | 0.800553 | 1.37E-48 | -1.69E-46 |
| MS.gene055952 | MS.gene79824 | 0.809545 | 1.79E-50 | -1.69E-46 |
| MS.gene055952 | MS.gene81047 | 0.815944 | 7.13E-52 | -1.69E-46 |
| MS.gene055952 | MS.gene82663 | 0.809977 | 1.45E-50 | -1.69E-46 |
| MS.gene055952 | MS.gene82668 | 0.82352 | 1.33E-53 | -1.69E-46 |
| MS.gene055952 | MS.gene82669 | 0.823254 | 1.53E-53 | -1.69E-46 |
| MS.gene055952 | MS.gene82682 | 0.803127 | 4.04E-49 | -1.69E-46 |
| MS.gene055952 | MS.gene88460 | 0.806307 | 8.77E-50 | -1.69E-46 |
| MS.gene055952 | MS.gene88533 | 0.815656 | 8.27E-52 | -1.69E-46 |
| MS.gene055952 | MS.gene88539 | 0.816912 | 4.33E-52 | -1.69E-46 |
| MS.gene055952 | MS.gene89666 | 0.836056 | 1.17E-56 | -1.69E-46 |
| MS.gene055952 | MS.gene91008 | 0.814873 | 1.23E-51 | -1.69E-46 |
| MS.gene055952 | MS.gene91451 | 0.814261 | 1.69E-51 | -1.69E-46 |
| MS.gene055952 | MS.gene91837 | 0.817838 | 2.68E-52 | -1.69E-46 |
| MS.gene055952 | MS.gene94237 | 0.835462 | 1.65E-56 | -1.69E-46 |
| MS.gene055952 | MS.gene96613 | 0.809697 | 1.66E-50 | -1.69E-46 |
| MS.gene055952 | MS.gene96684 | 0.858433 | 8.27E-63 | -1.69E-46 |
| MS.gene055952 | MS.gene98673 | 0.839062 | 1.98E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055952.t1 | MTR_1g064570 | 70.323 | 155 | 41 | 2 | 3 | 154 | 217 | 369 | 1.72e-64 | 202 |
| MS.gene055952.t1 | MTR_5g038260 | 46.358 | 151 | 70 | 2 | 4 | 154 | 213 | 352 | 3.89e-38 | 133 |
| MS.gene055952.t1 | MTR_5g038230 | 47.581 | 124 | 57 | 1 | 31 | 154 | 209 | 324 | 8.97e-33 | 119 |
| MS.gene055952.t1 | MTR_5g082780 | 42.222 | 135 | 61 | 2 | 18 | 152 | 113 | 230 | 2.69e-28 | 104 |
| MS.gene055952.t1 | MTR_1g108740 | 42.553 | 94 | 39 | 1 | 39 | 132 | 236 | 314 | 5.87e-17 | 76.6 |
| MS.gene055952.t1 | MTR_7g076110 | 41.379 | 116 | 49 | 6 | 18 | 130 | 169 | 268 | 3.53e-15 | 71.2 |
| MS.gene055952.t1 | MTR_7g077660 | 39.344 | 122 | 49 | 4 | 34 | 154 | 242 | 339 | 8.94e-15 | 70.5 |
| MS.gene055952.t1 | MTR_5g082770 | 47.945 | 73 | 29 | 1 | 37 | 109 | 227 | 290 | 1.77e-14 | 69.3 |
| MS.gene055952.t1 | MTR_7g076100 | 42.574 | 101 | 47 | 3 | 34 | 134 | 170 | 259 | 1.12e-13 | 67.0 |
| MS.gene055952.t1 | MTR_7g077620 | 36.538 | 104 | 54 | 2 | 34 | 137 | 243 | 334 | 4.46e-13 | 65.9 |
| MS.gene055952.t1 | MTR_1g016150 | 44.156 | 77 | 28 | 2 | 45 | 121 | 175 | 236 | 9.38e-12 | 61.2 |
| MS.gene055952.t1 | MTR_7g077650 | 38.095 | 105 | 50 | 3 | 34 | 137 | 247 | 337 | 1.44e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGAATAACAGACAAAATT+TGG | 0.158150 | 7.4:+15106587 | MS.gene055952:CDS |
| AATGTTAAGGCTTGATAGTT+TGG | 0.251290 | 7.4:+15106849 | MS.gene055952:CDS |
| AGCATATCTATTTAAATCAT+TGG | 0.316681 | 7.4:+15106684 | MS.gene055952:CDS |
| TTCATATCAAATGGTCCATT+CGG | 0.350533 | 7.4:-15107000 | None:intergenic |
| CGAAGTGTATAAGTTGGAAT+TGG | 0.356208 | 7.4:+15106801 | MS.gene055952:CDS |
| ATGTTAAGGCTTGATAGTTT+GGG | 0.380504 | 7.4:+15106850 | MS.gene055952:CDS |
| TCTTCATCGTCGTCGTCATC+AGG | 0.386056 | 7.4:-15106745 | None:intergenic |
| ATATCTAGTAGTGGTGCTAT+AGG | 0.404207 | 7.4:+15106775 | MS.gene055952:CDS |
| TCATATCAAATGGTCCATTC+GGG | 0.418206 | 7.4:-15106999 | None:intergenic |
| TTGGAATTGGATCTTCAAAC+TGG | 0.428934 | 7.4:+15106814 | MS.gene055952:CDS |
| TGGCTTGTAAGAAAAGCTAT+TGG | 0.434695 | 7.4:+15106718 | MS.gene055952:CDS |
| GTAAGCTTATACAAATGTTA+AGG | 0.439309 | 7.4:+15106836 | MS.gene055952:CDS |
| GGTCCATTCGGGTAAGGATG+TGG | 0.500949 | 7.4:-15106988 | None:intergenic |
| CCCAATGTTGTTTCATCGCG+AGG | 0.517264 | 7.4:+15106637 | MS.gene055952:CDS |
| GAACTCGAAGGATTGTGAAA+AGG | 0.517456 | 7.4:+15106609 | MS.gene055952:CDS |
| TGGTCTATGCCGTGGGACGT+TGG | 0.518643 | 7.4:+15106568 | None:intergenic |
| TCAAATGGTCCATTCGGGTA+AGG | 0.521367 | 7.4:-15106994 | None:intergenic |
| AGGATTCGAAGTGTATAAGT+TGG | 0.528528 | 7.4:+15106795 | MS.gene055952:CDS |
| CCCTCGCGATGAAACAACAT+TGG | 0.534928 | 7.4:-15106638 | None:intergenic |
| TCTGTTATTCCAACGTCCCA+CGG | 0.554175 | 7.4:-15106577 | None:intergenic |
| CCTCGCGATGAAACAACATT+GGG | 0.556507 | 7.4:-15106637 | None:intergenic |
| TATCTATTTAAATCATTGGA+GGG | 0.561312 | 7.4:+15106688 | MS.gene055952:CDS |
| TAAAGGCCTGGTCTATGCCG+TGG | 0.566400 | 7.4:+15106560 | None:intergenic |
| ATATCTATTTAAATCATTGG+AGG | 0.573961 | 7.4:+15106687 | MS.gene055952:CDS |
| CCAATGTTGTTTCATCGCGA+GGG | 0.596874 | 7.4:+15106638 | MS.gene055952:CDS |
| AGACAAAATTTGGAACTCGA+AGG | 0.603775 | 7.4:+15106597 | MS.gene055952:CDS |
| AAAGGCCTGGTCTATGCCGT+GGG | 0.605272 | 7.4:+15106561 | None:intergenic |
| GATAGTCATATATCTAGTAG+TGG | 0.626262 | 7.4:+15106766 | MS.gene055952:CDS |
| AATCATTGGAGGGAGACTTG+TGG | 0.628442 | 7.4:+15106698 | MS.gene055952:CDS |
| TGTCCACATCCTTACCCGAA+TGG | 0.630695 | 7.4:+15106985 | MS.gene055952:CDS |
| ACAGTCTTCATAGAAATCAT+CGG | 0.647279 | 7.4:-15106965 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTAAAGATTTTCATATCAAA+TGG | - | chr7.4:15107012-15107031 | None:intergenic | 15.0% |
| !! | AGCATATCTATTTAAATCAT+TGG | + | chr7.4:15106684-15106703 | MS.gene055952:CDS | 20.0% |
| !! | ATATCTATTTAAATCATTGG+AGG | + | chr7.4:15106687-15106706 | MS.gene055952:CDS | 20.0% |
| !! | TATCTATTTAAATCATTGGA+GGG | + | chr7.4:15106688-15106707 | MS.gene055952:CDS | 20.0% |
| ! | GTAAGCTTATACAAATGTTA+AGG | + | chr7.4:15106836-15106855 | MS.gene055952:CDS | 25.0% |
| ! | TTGGAATAACAGACAAAATT+TGG | + | chr7.4:15106587-15106606 | MS.gene055952:CDS | 25.0% |
| !!! | GGAGACAATGTTTTATTTAT+GGG | + | chr7.4:15106871-15106890 | MS.gene055952:CDS | 25.0% |
| ACAGTCTTCATAGAAATCAT+CGG | - | chr7.4:15106968-15106987 | None:intergenic | 30.0% | |
| GATAGTCATATATCTAGTAG+TGG | + | chr7.4:15106766-15106785 | MS.gene055952:CDS | 30.0% | |
| TTCATATCAAATGGTCCATT+CGG | - | chr7.4:15107003-15107022 | None:intergenic | 30.0% | |
| ! | AATGTTAAGGCTTGATAGTT+TGG | + | chr7.4:15106849-15106868 | MS.gene055952:CDS | 30.0% |
| ! | ATGTTAAGGCTTGATAGTTT+GGG | + | chr7.4:15106850-15106869 | MS.gene055952:CDS | 30.0% |
| !!! | GGGAGACAATGTTTTATTTA+TGG | + | chr7.4:15106870-15106889 | MS.gene055952:CDS | 30.0% |
| AGGATTCGAAGTGTATAAGT+TGG | + | chr7.4:15106795-15106814 | MS.gene055952:CDS | 35.0% | |
| TCATATCAAATGGTCCATTC+GGG | - | chr7.4:15107002-15107021 | None:intergenic | 35.0% | |
| TTGGAATTGGATCTTCAAAC+TGG | + | chr7.4:15106814-15106833 | MS.gene055952:CDS | 35.0% | |
| ! | AGACAAAATTTGGAACTCGA+AGG | + | chr7.4:15106597-15106616 | MS.gene055952:CDS | 35.0% |
| ! | CGAAGTGTATAAGTTGGAAT+TGG | + | chr7.4:15106801-15106820 | MS.gene055952:CDS | 35.0% |
| ! | TGGCTTGTAAGAAAAGCTAT+TGG | + | chr7.4:15106718-15106737 | MS.gene055952:CDS | 35.0% |
| !! | ATATCTAGTAGTGGTGCTAT+AGG | + | chr7.4:15106775-15106794 | MS.gene055952:CDS | 35.0% |
| GAACTCGAAGGATTGTGAAA+AGG | + | chr7.4:15106609-15106628 | MS.gene055952:CDS | 40.0% | |
| AATCATTGGAGGGAGACTTG+TGG | + | chr7.4:15106698-15106717 | MS.gene055952:CDS | 45.0% | |
| CCTCGCGATGAAACAACATT+GGG | - | chr7.4:15106640-15106659 | None:intergenic | 45.0% | |
| TCAAATGGTCCATTCGGGTA+AGG | - | chr7.4:15106997-15107016 | None:intergenic | 45.0% | |
| TCTGTTATTCCAACGTCCCA+CGG | - | chr7.4:15106580-15106599 | None:intergenic | 45.0% | |
| !! | CCAATGTTGTTTCATCGCGA+GGG | + | chr7.4:15106638-15106657 | MS.gene055952:CDS | 45.0% |
| CCCTCGCGATGAAACAACAT+TGG | - | chr7.4:15106641-15106660 | None:intergenic | 50.0% | |
| TCTTCATCGTCGTCGTCATC+AGG | - | chr7.4:15106748-15106767 | None:intergenic | 50.0% | |
| TGTCCACATCCTTACCCGAA+TGG | + | chr7.4:15106985-15107004 | MS.gene055952:CDS | 50.0% | |
| !! | CCCAATGTTGTTTCATCGCG+AGG | + | chr7.4:15106637-15106656 | MS.gene055952:CDS | 50.0% |
| GGTCCATTCGGGTAAGGATG+TGG | - | chr7.4:15106991-15107010 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 15106574 | 15107038 | 15106574 | ID=MS.gene055952 |
| chr7.4 | mRNA | 15106574 | 15107038 | 15106574 | ID=MS.gene055952.t1;Parent=MS.gene055952 |
| chr7.4 | exon | 15106574 | 15107038 | 15106574 | ID=MS.gene055952.t1.exon1;Parent=MS.gene055952.t1 |
| chr7.4 | CDS | 15106574 | 15107038 | 15106574 | ID=cds.MS.gene055952.t1;Parent=MS.gene055952.t1 |
| Gene Sequence |
| Protein sequence |
