Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92065.t1 | XP_003596709.2 | 91.3 | 231 | 20 | 0 | 1 | 231 | 1 | 231 | 2.80E-111 | 411.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92065.t1 | A0A072VA27 | 91.3 | 231 | 20 | 0 | 1 | 231 | 1 | 231 | 2.0e-111 | 411.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056677 | MS.gene92065 | 0.814702 | 1.35E-51 | -1.69E-46 |
| MS.gene058279 | MS.gene92065 | 0.808253 | 3.39E-50 | -1.69E-46 |
| MS.gene05952 | MS.gene92065 | 0.815442 | 9.23E-52 | -1.69E-46 |
| MS.gene060012 | MS.gene92065 | 0.812097 | 5.03E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92065.t1 | MTR_2g083910 | 96.970 | 231 | 7 | 0 | 1 | 231 | 1 | 231 | 1.82e-163 | 451 |
| MS.gene92065.t1 | MTR_2g083910 | 96.970 | 231 | 7 | 0 | 1 | 231 | 1 | 231 | 6.70e-163 | 451 |
| MS.gene92065.t1 | MTR_4g080340 | 50.000 | 258 | 81 | 6 | 1 | 231 | 1 | 237 | 4.56e-62 | 194 |
| MS.gene92065.t1 | MTR_8g086820 | 44.715 | 246 | 113 | 7 | 1 | 230 | 1 | 239 | 3.67e-54 | 174 |
| MS.gene92065.t1 | MTR_2g016760 | 44.118 | 238 | 115 | 5 | 1 | 230 | 1 | 228 | 3.85e-53 | 176 |
| MS.gene92065.t1 | MTR_2g078620 | 47.059 | 102 | 42 | 5 | 134 | 230 | 225 | 319 | 1.66e-18 | 82.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92065.t1 | AT2G42760 | 37.407 | 270 | 122 | 9 | 1 | 230 | 1 | 263 | 4.80e-35 | 125 |
Find 37 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTATCAGAGCTTGAGTTTA+AGG | 0.133074 | 2.1:-17596561 | MS.gene92065:CDS |
| TAAGGAGCTAAAAGGGTTTA+TGG | 0.192552 | 2.1:-17596543 | MS.gene92065:CDS |
| CTTGAGTTTAAGGAGCTAAA+AGG | 0.195416 | 2.1:-17596551 | MS.gene92065:CDS |
| TTGTTAATCCATTGCTTAAT+TGG | 0.205608 | 2.1:-17596370 | MS.gene92065:CDS |
| GCTAAAAGGGTTTATGGATT+TGG | 0.214083 | 2.1:-17596537 | MS.gene92065:CDS |
| TGAAAGATAATCTTAAGTTT+TGG | 0.252639 | 2.1:-17596316 | MS.gene92065:CDS |
| CTAAAAGGGTTTATGGATTT+GGG | 0.263401 | 2.1:-17596536 | MS.gene92065:CDS |
| GCCTTATTTATCTGAGGCTT+GGG | 0.273923 | 2.1:-17596414 | MS.gene92065:CDS |
| TCTGAGGAAGATAAAGATTC+AGG | 0.292013 | 2.1:-17596503 | MS.gene92065:CDS |
| ACTTCTTTGCCTGAGAGAAT+TGG | 0.350310 | 2.1:+17596683 | None:intergenic |
| GGCCTTATTTATCTGAGGCT+TGG | 0.364492 | 2.1:-17596415 | MS.gene92065:CDS |
| GAATTCCAACACTTCAAGTT+AGG | 0.372690 | 2.1:-17596796 | MS.gene92065:CDS |
| CCCCAAGCCTCAGATAAATA+AGG | 0.382221 | 2.1:+17596413 | None:intergenic |
| TGAAACTGTTTGATATCAAC+TGG | 0.384594 | 2.1:-17596946 | MS.gene92065:CDS |
| GAAAACTTAGGCACGAAGAT+TGG | 0.434650 | 2.1:-17596761 | MS.gene92065:CDS |
| GGATTGGTTTCTTTGATACC+AGG | 0.467028 | 2.1:-17596482 | MS.gene92065:CDS |
| TGTGATGTGAGGACAGAGTT+TGG | 0.472758 | 2.1:+17596716 | None:intergenic |
| TTGAGTTTAAGGAGCTAAAA+GGG | 0.477612 | 2.1:-17596550 | MS.gene92065:CDS |
| AATTGGAGGGTACCAACTCT+TGG | 0.482818 | 2.1:-17596353 | MS.gene92065:CDS |
| AAGCTGAAGCCAATTCTCTC+AGG | 0.492170 | 2.1:-17596692 | MS.gene92065:CDS |
| TTCTTCCACTTGAGACATAG+AGG | 0.502500 | 2.1:+17596847 | None:intergenic |
| CACTTTCATCAACATTGTGT+TGG | 0.505818 | 2.1:+17596447 | None:intergenic |
| AAAGACCTAACTTGAAGTGT+TGG | 0.513872 | 2.1:+17596791 | None:intergenic |
| TTAATCCATTGCTTAATTGG+AGG | 0.514757 | 2.1:-17596367 | MS.gene92065:CDS |
| TTGATGAAAGTGTGATTAGT+AGG | 0.517023 | 2.1:-17596436 | MS.gene92065:CDS |
| GGAAGATAAAGATTCAGGAT+TGG | 0.523132 | 2.1:-17596498 | MS.gene92065:CDS |
| TAATCCATTGCTTAATTGGA+GGG | 0.524602 | 2.1:-17596366 | MS.gene92065:CDS |
| CATAGAACGAGACTTAGAAA+AGG | 0.547413 | 2.1:-17596602 | MS.gene92065:CDS |
| TAGTAGGCCTTATTTATCTG+AGG | 0.548733 | 2.1:-17596420 | MS.gene92065:CDS |
| CCTTATTTATCTGAGGCTTG+GGG | 0.550864 | 2.1:-17596413 | MS.gene92065:CDS |
| TCAATCTCATTGCCAAGAGT+TGG | 0.554919 | 2.1:+17596341 | None:intergenic |
| TTCAACCTCTATGTCTCAAG+TGG | 0.558037 | 2.1:-17596852 | MS.gene92065:CDS |
| AGTAATGTTGTTGAATGTAA+AGG | 0.562510 | 2.1:+17596881 | None:intergenic |
| GATTGGTTTCTTTGATACCA+GGG | 0.577636 | 2.1:-17596481 | MS.gene92065:CDS |
| AATTCTCTCAGGCAAAGAAG+TGG | 0.596666 | 2.1:-17596681 | MS.gene92065:CDS |
| GGTACCCTCCAATTAAGCAA+TGG | 0.611167 | 2.1:+17596362 | None:intergenic |
| GCTTCAGCTTCTGTGATGTG+AGG | 0.642042 | 2.1:+17596705 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAAAGATAATCTTAAGTTTT+GGG | - | chr2.1:17596945-17596964 | MS.gene92065:CDS | 20.0% |
| !!! | TGAAAGATAATCTTAAGTTT+TGG | - | chr2.1:17596944-17596963 | MS.gene92065:CDS | 20.0% |
| ! | AGTAATGTTGTTGAATGTAA+AGG | + | chr2.1:17596382-17596401 | None:intergenic | 25.0% |
| ! | TTGTTAATCCATTGCTTAAT+TGG | - | chr2.1:17596890-17596909 | MS.gene92065:CDS | 25.0% |
| !!! | TGAAGATGTTTTTTTGTTGT+TGG | + | chr2.1:17596359-17596378 | None:intergenic | 25.0% |
| AGATGTAACCGATACAAAAA+AGG | - | chr2.1:17596621-17596640 | MS.gene92065:CDS | 30.0% | |
| CTAAAAGGGTTTATGGATTT+GGG | - | chr2.1:17596724-17596743 | MS.gene92065:CDS | 30.0% | |
| TAATCCATTGCTTAATTGGA+GGG | - | chr2.1:17596894-17596913 | MS.gene92065:CDS | 30.0% | |
| TGAAACTGTTTGATATCAAC+TGG | - | chr2.1:17596314-17596333 | MS.gene92065:CDS | 30.0% | |
| TTAATCCATTGCTTAATTGG+AGG | - | chr2.1:17596893-17596912 | MS.gene92065:CDS | 30.0% | |
| TTGAGTTTAAGGAGCTAAAA+GGG | - | chr2.1:17596710-17596729 | MS.gene92065:CDS | 30.0% | |
| TTTATCAGAGCTTGAGTTTA+AGG | - | chr2.1:17596699-17596718 | MS.gene92065:CDS | 30.0% | |
| ! | TTGATGAAAGTGTGATTAGT+AGG | - | chr2.1:17596824-17596843 | MS.gene92065:CDS | 30.0% |
| AAAGACCTAACTTGAAGTGT+TGG | + | chr2.1:17596472-17596491 | None:intergenic | 35.0% | |
| CACTTTCATCAACATTGTGT+TGG | + | chr2.1:17596816-17596835 | None:intergenic | 35.0% | |
| CATAGAACGAGACTTAGAAA+AGG | - | chr2.1:17596658-17596677 | MS.gene92065:CDS | 35.0% | |
| CTTGAGTTTAAGGAGCTAAA+AGG | - | chr2.1:17596709-17596728 | MS.gene92065:CDS | 35.0% | |
| GAAAAGGAAGAAAAACTGCT+AGG | - | chr2.1:17596674-17596693 | MS.gene92065:CDS | 35.0% | |
| GCTAAAAGGGTTTATGGATT+TGG | - | chr2.1:17596723-17596742 | MS.gene92065:CDS | 35.0% | |
| GGAAGATAAAGATTCAGGAT+TGG | - | chr2.1:17596762-17596781 | MS.gene92065:CDS | 35.0% | |
| TAAGGAGCTAAAAGGGTTTA+TGG | - | chr2.1:17596717-17596736 | MS.gene92065:CDS | 35.0% | |
| TCTGAGGAAGATAAAGATTC+AGG | - | chr2.1:17596757-17596776 | MS.gene92065:CDS | 35.0% | |
| ! | GAATTCCAACACTTCAAGTT+AGG | - | chr2.1:17596464-17596483 | MS.gene92065:CDS | 35.0% |
| ! | TCTTTTAGCGACGAAAACTT+AGG | - | chr2.1:17596487-17596506 | MS.gene92065:CDS | 35.0% |
| !! | GATTGGTTTCTTTGATACCA+GGG | - | chr2.1:17596779-17596798 | MS.gene92065:CDS | 35.0% |
| !! | TAGTAGGCCTTATTTATCTG+AGG | - | chr2.1:17596840-17596859 | MS.gene92065:CDS | 35.0% |
| !! | TTTGGGATTTGTGTTTTCTG+AGG | - | chr2.1:17596741-17596760 | MS.gene92065:CDS | 35.0% |
| !!! | ATGACTCGCCTTTTTTGTAT+CGG | + | chr2.1:17596632-17596651 | None:intergenic | 35.0% |
| AATTCTCTCAGGCAAAGAAG+TGG | - | chr2.1:17596579-17596598 | MS.gene92065:CDS | 40.0% | |
| ACTTCTTTGCCTGAGAGAAT+TGG | + | chr2.1:17596580-17596599 | None:intergenic | 40.0% | |
| GAAAACTTAGGCACGAAGAT+TGG | - | chr2.1:17596499-17596518 | MS.gene92065:CDS | 40.0% | |
| TCAATCTCATTGCCAAGAGT+TGG | + | chr2.1:17596922-17596941 | None:intergenic | 40.0% | |
| TTCAACCTCTATGTCTCAAG+TGG | - | chr2.1:17596408-17596427 | MS.gene92065:CDS | 40.0% | |
| TTCTTCCACTTGAGACATAG+AGG | + | chr2.1:17596416-17596435 | None:intergenic | 40.0% | |
| ! | CCTTATTTATCTGAGGCTTG+GGG | - | chr2.1:17596847-17596866 | MS.gene92065:CDS | 40.0% |
| !! | GCCTTATTTATCTGAGGCTT+GGG | - | chr2.1:17596846-17596865 | MS.gene92065:CDS | 40.0% |
| !! | GGATTGGTTTCTTTGATACC+AGG | - | chr2.1:17596778-17596797 | MS.gene92065:CDS | 40.0% |
| AAGCTGAAGCCAATTCTCTC+AGG | - | chr2.1:17596568-17596587 | MS.gene92065:CDS | 45.0% | |
| CCCCAAGCCTCAGATAAATA+AGG | + | chr2.1:17596850-17596869 | None:intergenic | 45.0% | |
| GGTACCCTCCAATTAAGCAA+TGG | + | chr2.1:17596901-17596920 | None:intergenic | 45.0% | |
| TGTGATGTGAGGACAGAGTT+TGG | + | chr2.1:17596547-17596566 | None:intergenic | 45.0% | |
| !! | AATTGGAGGGTACCAACTCT+TGG | - | chr2.1:17596907-17596926 | MS.gene92065:CDS | 45.0% |
| !! | GGCCTTATTTATCTGAGGCT+TGG | - | chr2.1:17596845-17596864 | MS.gene92065:CDS | 45.0% |
| !!! | TGTTGGTTTTTGCTCAACCC+TGG | + | chr2.1:17596799-17596818 | None:intergenic | 45.0% |
| GCTTCAGCTTCTGTGATGTG+AGG | + | chr2.1:17596558-17596577 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 17596295 | 17596987 | 17596295 | ID=MS.gene92065 |
| chr2.1 | mRNA | 17596295 | 17596987 | 17596295 | ID=MS.gene92065.t1;Parent=MS.gene92065 |
| chr2.1 | exon | 17596295 | 17596987 | 17596295 | ID=MS.gene92065.t1.exon1;Parent=MS.gene92065.t1 |
| chr2.1 | CDS | 17596295 | 17596987 | 17596295 | ID=cds.MS.gene92065.t1;Parent=MS.gene92065.t1 |
| Gene Sequence |
| Protein sequence |
