Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92067.t1 | XP_003596709.2 | 91.4 | 233 | 20 | 0 | 1 | 233 | 1 | 233 | 2.60E-112 | 414.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92067.t1 | G7IM74 | 91.4 | 233 | 20 | 0 | 1 | 233 | 1 | 233 | 1.8e-112 | 414.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056677 | MS.gene92067 | 0.808682 | 2.75E-50 | -1.69E-46 |
| MS.gene058279 | MS.gene92067 | 0.804711 | 1.89E-49 | -1.69E-46 |
| MS.gene05952 | MS.gene92067 | 0.816836 | 4.50E-52 | -1.69E-46 |
| MS.gene060012 | MS.gene92067 | 0.814454 | 1.53E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92067.t1 | MTR_2g083910 | 96.996 | 233 | 7 | 0 | 1 | 233 | 1 | 233 | 5.07e-165 | 455 |
| MS.gene92067.t1 | MTR_2g083910 | 96.970 | 231 | 7 | 0 | 1 | 231 | 1 | 231 | 7.26e-163 | 451 |
| MS.gene92067.t1 | MTR_4g080340 | 50.000 | 260 | 82 | 6 | 1 | 233 | 1 | 239 | 4.10e-63 | 197 |
| MS.gene92067.t1 | MTR_8g086820 | 45.382 | 249 | 113 | 7 | 1 | 233 | 1 | 242 | 1.14e-55 | 178 |
| MS.gene92067.t1 | MTR_2g016760 | 44.167 | 240 | 116 | 5 | 1 | 232 | 1 | 230 | 1.27e-53 | 177 |
| MS.gene92067.t1 | MTR_2g078620 | 47.059 | 102 | 42 | 5 | 134 | 230 | 225 | 319 | 1.70e-18 | 82.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92067.t1 | AT2G42760 | 37.363 | 273 | 124 | 9 | 1 | 233 | 1 | 266 | 2.96e-36 | 129 |
Find 38 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTATCAGAGCTTGAGTTTA+AGG | 0.133074 | 2.1:-17582866 | MS.gene92067:CDS |
| TAAGGAGCTAAAAGGGTTTA+TGG | 0.192552 | 2.1:-17582848 | MS.gene92067:CDS |
| CTTGAGTTTAAGGAGCTAAA+AGG | 0.195416 | 2.1:-17582856 | MS.gene92067:CDS |
| TTGTTAATCCATTGCTTAAT+TGG | 0.205608 | 2.1:-17582675 | MS.gene92067:CDS |
| GCTAAAAGGGTTTATGGATT+TGG | 0.214083 | 2.1:-17582842 | MS.gene92067:CDS |
| TGAAAGATAATCTTAAGTTT+TGG | 0.252639 | 2.1:-17582621 | MS.gene92067:CDS |
| CTAAAAGGGTTTATGGATTT+GGG | 0.263401 | 2.1:-17582841 | MS.gene92067:CDS |
| GCCTTATTTATCTGAGGCTT+GGG | 0.273923 | 2.1:-17582719 | MS.gene92067:CDS |
| TCTGAGGAAGATAAAGATTC+AGG | 0.292013 | 2.1:-17582808 | MS.gene92067:CDS |
| ACTTCTTTGCCTGAGAGAAT+TGG | 0.350310 | 2.1:+17582988 | None:intergenic |
| GGCCTTATTTATCTGAGGCT+TGG | 0.364492 | 2.1:-17582720 | MS.gene92067:CDS |
| GAATTCCAACACTTCAAGTT+AGG | 0.372690 | 2.1:-17583101 | MS.gene92067:CDS |
| CCCCAAGCCTCAGATAAATA+AGG | 0.382221 | 2.1:+17582718 | None:intergenic |
| TGAAACTGTTTGATATCAAC+TGG | 0.384594 | 2.1:-17583251 | MS.gene92067:CDS |
| GAAAACTTAGGCACGAAGAT+TGG | 0.434650 | 2.1:-17583066 | MS.gene92067:CDS |
| GGATTGGTTTCTTTGATACC+AGG | 0.467028 | 2.1:-17582787 | MS.gene92067:CDS |
| TGTGATGTGAGGACAGAGTT+TGG | 0.472758 | 2.1:+17583021 | None:intergenic |
| TTGAGTTTAAGGAGCTAAAA+GGG | 0.477612 | 2.1:-17582855 | MS.gene92067:CDS |
| AATTGGAGGGTACCAACTCT+TGG | 0.482818 | 2.1:-17582658 | MS.gene92067:CDS |
| AAGCTGAAGCCAATTCTCTC+AGG | 0.492170 | 2.1:-17582997 | MS.gene92067:CDS |
| TTCTTCCACTTGAGACATAG+AGG | 0.502500 | 2.1:+17583152 | None:intergenic |
| CACTTTCATCAACATTGTGT+TGG | 0.505818 | 2.1:+17582752 | None:intergenic |
| AAAGACCTAACTTGAAGTGT+TGG | 0.513872 | 2.1:+17583096 | None:intergenic |
| TTAATCCATTGCTTAATTGG+AGG | 0.514757 | 2.1:-17582672 | MS.gene92067:CDS |
| TTGATGAAAGTGTGATTAGT+AGG | 0.517023 | 2.1:-17582741 | MS.gene92067:CDS |
| GGAAGATAAAGATTCAGGAT+TGG | 0.523132 | 2.1:-17582803 | MS.gene92067:CDS |
| TAATCCATTGCTTAATTGGA+GGG | 0.524602 | 2.1:-17582671 | MS.gene92067:CDS |
| CATAGAACGAGACTTAGAAA+AGG | 0.547413 | 2.1:-17582907 | MS.gene92067:CDS |
| TAGTAGGCCTTATTTATCTG+AGG | 0.548733 | 2.1:-17582725 | MS.gene92067:CDS |
| CCTTATTTATCTGAGGCTTG+GGG | 0.550864 | 2.1:-17582718 | MS.gene92067:CDS |
| TCAATCTCATTGCCAAGAGT+TGG | 0.554919 | 2.1:+17582646 | None:intergenic |
| TTCAACCTCTATGTCTCAAG+TGG | 0.558037 | 2.1:-17583157 | MS.gene92067:CDS |
| AGTAATGTTGTTGAATGTAA+AGG | 0.562510 | 2.1:+17583186 | None:intergenic |
| GATTGGTTTCTTTGATACCA+GGG | 0.577636 | 2.1:-17582786 | MS.gene92067:CDS |
| TGTTGATTGTTATCTTACAA+TGG | 0.583010 | 2.1:+17582582 | None:intergenic |
| AATTCTCTCAGGCAAAGAAG+TGG | 0.596666 | 2.1:-17582986 | MS.gene92067:CDS |
| GGTACCCTCCAATTAAGCAA+TGG | 0.611167 | 2.1:+17582667 | None:intergenic |
| GCTTCAGCTTCTGTGATGTG+AGG | 0.642042 | 2.1:+17583010 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAAAGATAATCTTAAGTTTT+GGG | - | chr2.1:17583241-17583260 | MS.gene92067:CDS | 20.0% |
| !!! | TGAAAGATAATCTTAAGTTT+TGG | - | chr2.1:17583240-17583259 | MS.gene92067:CDS | 20.0% |
| ! | AGTAATGTTGTTGAATGTAA+AGG | + | chr2.1:17582678-17582697 | None:intergenic | 25.0% |
| ! | TTGTTAATCCATTGCTTAAT+TGG | - | chr2.1:17583186-17583205 | MS.gene92067:CDS | 25.0% |
| !!! | TGAAGATGTTTTTTTGTTGT+TGG | + | chr2.1:17582655-17582674 | None:intergenic | 25.0% |
| AGATGTAACCGATACAAAAA+AGG | - | chr2.1:17582917-17582936 | MS.gene92067:CDS | 30.0% | |
| CTAAAAGGGTTTATGGATTT+GGG | - | chr2.1:17583020-17583039 | MS.gene92067:CDS | 30.0% | |
| TAATCCATTGCTTAATTGGA+GGG | - | chr2.1:17583190-17583209 | MS.gene92067:CDS | 30.0% | |
| TGAAACTGTTTGATATCAAC+TGG | - | chr2.1:17582610-17582629 | MS.gene92067:CDS | 30.0% | |
| TTAATCCATTGCTTAATTGG+AGG | - | chr2.1:17583189-17583208 | MS.gene92067:CDS | 30.0% | |
| TTGAGTTTAAGGAGCTAAAA+GGG | - | chr2.1:17583006-17583025 | MS.gene92067:CDS | 30.0% | |
| TTTATCAGAGCTTGAGTTTA+AGG | - | chr2.1:17582995-17583014 | MS.gene92067:CDS | 30.0% | |
| ! | TTGATGAAAGTGTGATTAGT+AGG | - | chr2.1:17583120-17583139 | MS.gene92067:CDS | 30.0% |
| AAAGACCTAACTTGAAGTGT+TGG | + | chr2.1:17582768-17582787 | None:intergenic | 35.0% | |
| CACTTTCATCAACATTGTGT+TGG | + | chr2.1:17583112-17583131 | None:intergenic | 35.0% | |
| CATAGAACGAGACTTAGAAA+AGG | - | chr2.1:17582954-17582973 | MS.gene92067:CDS | 35.0% | |
| CTTGAGTTTAAGGAGCTAAA+AGG | - | chr2.1:17583005-17583024 | MS.gene92067:CDS | 35.0% | |
| GAAAAGGAAGAAAAACTGCT+AGG | - | chr2.1:17582970-17582989 | MS.gene92067:CDS | 35.0% | |
| GCTAAAAGGGTTTATGGATT+TGG | - | chr2.1:17583019-17583038 | MS.gene92067:CDS | 35.0% | |
| GGAAGATAAAGATTCAGGAT+TGG | - | chr2.1:17583058-17583077 | MS.gene92067:CDS | 35.0% | |
| TAAGGAGCTAAAAGGGTTTA+TGG | - | chr2.1:17583013-17583032 | MS.gene92067:CDS | 35.0% | |
| TCTGAGGAAGATAAAGATTC+AGG | - | chr2.1:17583053-17583072 | MS.gene92067:CDS | 35.0% | |
| ! | GAATTCCAACACTTCAAGTT+AGG | - | chr2.1:17582760-17582779 | MS.gene92067:CDS | 35.0% |
| ! | TCTTTTAGCGACGAAAACTT+AGG | - | chr2.1:17582783-17582802 | MS.gene92067:CDS | 35.0% |
| !! | GATTGGTTTCTTTGATACCA+GGG | - | chr2.1:17583075-17583094 | MS.gene92067:CDS | 35.0% |
| !! | TAGTAGGCCTTATTTATCTG+AGG | - | chr2.1:17583136-17583155 | MS.gene92067:CDS | 35.0% |
| !! | TTTGGGATTTGTGTTTTCTG+AGG | - | chr2.1:17583037-17583056 | MS.gene92067:CDS | 35.0% |
| !!! | ATGACTCGCCTTTTTTGTAT+CGG | + | chr2.1:17582928-17582947 | None:intergenic | 35.0% |
| AATTCTCTCAGGCAAAGAAG+TGG | - | chr2.1:17582875-17582894 | MS.gene92067:CDS | 40.0% | |
| ACTTCTTTGCCTGAGAGAAT+TGG | + | chr2.1:17582876-17582895 | None:intergenic | 40.0% | |
| GAAAACTTAGGCACGAAGAT+TGG | - | chr2.1:17582795-17582814 | MS.gene92067:CDS | 40.0% | |
| TCAATCTCATTGCCAAGAGT+TGG | + | chr2.1:17583218-17583237 | None:intergenic | 40.0% | |
| TTCAACCTCTATGTCTCAAG+TGG | - | chr2.1:17582704-17582723 | MS.gene92067:CDS | 40.0% | |
| TTCTTCCACTTGAGACATAG+AGG | + | chr2.1:17582712-17582731 | None:intergenic | 40.0% | |
| ! | CCTTATTTATCTGAGGCTTG+GGG | - | chr2.1:17583143-17583162 | MS.gene92067:CDS | 40.0% |
| !! | GCCTTATTTATCTGAGGCTT+GGG | - | chr2.1:17583142-17583161 | MS.gene92067:CDS | 40.0% |
| !! | GGATTGGTTTCTTTGATACC+AGG | - | chr2.1:17583074-17583093 | MS.gene92067:CDS | 40.0% |
| AAGCTGAAGCCAATTCTCTC+AGG | - | chr2.1:17582864-17582883 | MS.gene92067:CDS | 45.0% | |
| CCCCAAGCCTCAGATAAATA+AGG | + | chr2.1:17583146-17583165 | None:intergenic | 45.0% | |
| GGTACCCTCCAATTAAGCAA+TGG | + | chr2.1:17583197-17583216 | None:intergenic | 45.0% | |
| TGTGATGTGAGGACAGAGTT+TGG | + | chr2.1:17582843-17582862 | None:intergenic | 45.0% | |
| !! | AATTGGAGGGTACCAACTCT+TGG | - | chr2.1:17583203-17583222 | MS.gene92067:CDS | 45.0% |
| !! | GGCCTTATTTATCTGAGGCT+TGG | - | chr2.1:17583141-17583160 | MS.gene92067:CDS | 45.0% |
| !!! | TGTTGGTTTTTGCTCAACCC+TGG | + | chr2.1:17583095-17583114 | None:intergenic | 45.0% |
| GCTTCAGCTTCTGTGATGTG+AGG | + | chr2.1:17582854-17582873 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 17582591 | 17583292 | 17582591 | ID=MS.gene92067 |
| chr2.1 | mRNA | 17582591 | 17583292 | 17582591 | ID=MS.gene92067.t1;Parent=MS.gene92067 |
| chr2.1 | exon | 17582591 | 17583292 | 17582591 | ID=MS.gene92067.t1.exon1;Parent=MS.gene92067.t1 |
| chr2.1 | CDS | 17582591 | 17583292 | 17582591 | ID=cds.MS.gene92067.t1;Parent=MS.gene92067.t1 |
| Gene Sequence |
| Protein sequence |
