AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92329

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92329.t1 MTR_5g005600 94.061 724 40 1 1 724 1 721 0.0 1380
MS.gene92329.t1 MTR_2g088470 52.535 710 301 13 23 719 46 732 0.0 697
MS.gene92329.t1 MTR_4g047800 51.909 707 295 13 32 719 49 729 0.0 680
MS.gene92329.t1 MTR_8g432540 42.799 743 377 14 12 719 77 806 0.0 575
MS.gene92329.t1 MTR_7g076080 42.595 709 381 12 29 719 74 774 0.0 560
MS.gene92329.t1 MTR_1g101280 39.463 707 388 14 43 719 54 750 2.25e-173 515
MS.gene92329.t1 MTR_2g101720 38.653 683 371 13 49 719 143 789 1.43e-157 476
MS.gene92329.t1 MTR_0198s0030 37.964 727 384 16 37 719 48 751 1.63e-155 469
MS.gene92329.t1 MTR_6g477820 36.541 717 391 16 45 719 29 723 1.17e-148 451
MS.gene92329.t1 MTR_5g029320 35.463 767 424 22 1 719 35 778 1.15e-142 437
MS.gene92329.t1 MTR_7g427710 35.887 705 340 20 45 719 16 638 4.47e-123 382
MS.gene92329.t1 MTR_2g030570 33.569 706 404 20 46 720 25 696 4.11e-110 350
MS.gene92329.t1 MTR_2g030600 32.656 689 428 16 45 720 24 689 3.10e-104 334
MS.gene92329.t1 MTR_2g030660 30.825 691 428 16 34 720 4 648 2.84e-85 283
MS.gene92329.t1 MTR_2g083350 32.892 529 296 11 233 719 20 531 8.05e-81 268
MS.gene92329.t1 MTR_2g083330 34.240 441 265 6 286 719 103 525 6.69e-78 260
MS.gene92329.t1 MTR_7g427690 35.322 419 224 13 213 614 38 426 2.58e-71 240
MS.gene92329.t1 MTR_7g427700 32.484 314 150 9 45 346 16 279 2.16e-37 145
MS.gene92329.t1 MTR_7g427700 38.750 160 76 4 573 719 281 431 1.19e-19 92.8
MS.gene92329.t1 MTR_0653s0020 36.548 197 101 5 415 607 1 177 2.63e-34 130
MS.gene92329.t1 MTR_2g030690 30.420 286 188 7 438 720 1 278 9.81e-27 110
MS.gene92329.t1 MTR_5g046040 32.461 191 97 6 45 221 33 205 5.16e-22 99.0
MS.gene92329.t1 MTR_7g027265 36.364 121 76 1 44 163 16 136 3.71e-21 90.5
MS.gene92329.t1 MTR_1g105420 36.649 191 106 8 100 285 1 181 1.03e-20 91.7
MS.gene92329.t1 MTR_1g105420 73.171 41 10 1 347 386 171 211 4.91e-13 69.3
MS.gene92329.t1 MTR_1g105425 45.946 74 40 0 18 91 79 152 1.60e-15 74.7
MS.gene92329.t1 MTR_0653s0010 41.322 121 51 3 612 719 1 114 6.95e-15 72.0
MS.gene92329.t1 MTR_2g451350 35.385 130 83 1 242 370 1 130 6.98e-15 72.4
MS.gene92329.t1 MTR_7g027605 41.053 95 50 2 318 412 3 91 1.12e-14 70.5
MS.gene92329.t1 MTR_6g027290 30.827 133 89 3 36 166 17 148 1.45e-14 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92329.t1 AT4G04890 54.457 718 286 13 23 719 38 735 0.0 755
MS.gene92329.t1 AT4G04890 54.457 718 286 13 23 719 38 735 0.0 755
MS.gene92329.t1 AT4G21750 53.397 736 288 14 27 723 42 761 0.0 754
MS.gene92329.t1 AT4G21750 53.397 736 288 14 27 723 42 761 0.0 754
MS.gene92329.t1 AT4G21750 53.397 736 288 14 27 723 42 761 0.0 754
MS.gene92329.t1 AT4G21750 53.397 736 288 14 27 723 42 761 0.0 754
MS.gene92329.t1 AT1G05230 49.858 704 305 11 29 719 48 716 0.0 674
MS.gene92329.t1 AT1G05230 49.858 704 305 11 29 719 48 716 0.0 674
MS.gene92329.t1 AT1G05230 49.858 704 305 11 29 719 48 716 0.0 674
MS.gene92329.t1 AT1G05230 49.858 704 305 11 29 719 48 716 0.0 674
MS.gene92329.t1 AT1G05230 49.858 704 305 11 29 719 48 716 0.0 674
MS.gene92329.t1 AT1G05230 49.858 704 305 11 29 719 48 716 0.0 674
MS.gene92329.t1 AT1G05230 49.858 704 305 11 29 719 48 716 0.0 674
MS.gene92329.t1 AT1G05230 49.858 704 305 11 29 719 48 716 0.0 674
MS.gene92329.t1 AT1G05230 49.858 704 305 11 29 719 48 716 0.0 674
MS.gene92329.t1 AT1G05230 49.858 704 305 11 29 719 48 716 0.0 674
MS.gene92329.t1 AT1G05230 49.858 704 303 12 29 719 48 714 0.0 667
MS.gene92329.t1 AT4G00730 42.324 723 367 15 20 719 103 798 0.0 539
MS.gene92329.t1 AT1G05230 48.211 587 259 10 29 602 48 602 0.0 539
MS.gene92329.t1 AT3G61150 39.501 762 380 17 8 719 73 803 1.52e-177 528
MS.gene92329.t1 AT2G32370 40.863 695 359 17 36 716 61 717 3.39e-161 483
MS.gene92329.t1 AT5G52170 38.495 691 356 12 37 719 49 678 4.31e-156 468
MS.gene92329.t1 AT5G52170 38.495 691 356 12 37 719 49 678 4.31e-156 468
MS.gene92329.t1 AT5G52170 38.295 692 361 12 37 719 49 683 1.83e-154 464
MS.gene92329.t1 AT1G73360 38.008 713 373 17 45 719 32 713 3.75e-146 444
MS.gene92329.t1 AT1G17920 38.394 685 380 11 49 719 25 681 8.10e-145 439
MS.gene92329.t1 AT1G17920 38.394 685 380 11 49 719 25 681 8.10e-145 439
MS.gene92329.t1 AT1G79840 37.698 695 367 18 41 719 40 684 1.57e-144 439
MS.gene92329.t1 AT1G79840 37.698 695 367 18 41 719 40 684 1.57e-144 439
MS.gene92329.t1 AT1G79840 37.698 695 367 18 41 719 98 742 7.56e-144 439
MS.gene92329.t1 AT1G79840 37.698 695 367 18 41 719 127 771 2.43e-143 439
MS.gene92329.t1 AT5G46880 35.049 719 417 20 36 719 103 806 1.74e-126 396
MS.gene92329.t1 AT5G46880 35.049 719 417 20 36 719 103 806 1.74e-126 396
MS.gene92329.t1 AT5G46880 35.049 719 417 20 36 719 103 806 1.74e-126 396
MS.gene92329.t1 AT1G79840 37.786 569 298 15 41 599 98 620 1.32e-118 370
MS.gene92329.t1 AT3G03260 33.807 704 397 21 46 716 24 691 2.62e-112 355
MS.gene92329.t1 AT4G17710 34.483 696 372 20 37 720 81 704 4.22e-111 352
MS.gene92329.t1 AT4G25530 33.478 693 413 18 34 719 31 682 1.84e-108 345
MS.gene92329.t1 AT4G00730 39.407 472 246 10 20 473 103 552 1.65e-99 318
MS.gene92329.t1 AT5G17320 31.944 720 431 23 37 719 18 715 1.53e-94 309
MS.gene92329.t1 AT1G34650 31.312 709 424 20 50 719 21 705 1.88e-88 293
MS.gene92329.t1 AT1G79840 38.600 443 219 13 41 474 98 496 5.10e-87 283
MS.gene92329.t1 AT3G03260 32.844 545 317 17 46 571 24 538 1.21e-79 265
MS.gene92329.t1 AT5G07260 26.212 557 329 22 111 660 10 491 2.23e-29 123
MS.gene92329.t1 AT5G07260 26.126 555 327 21 111 660 10 486 1.06e-28 121

Find 192 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTCACGCTCTGCTGATTT+TGG 0.190379 5.1:+614165 MS.gene92329:CDS
GTTAATTCTGGACTTGCTTT+TGG 0.195569 5.1:+615355 MS.gene92329:CDS
AACTGCATCCAGATGGAATA+TGG 0.242195 5.1:+615014 MS.gene92329:CDS
TCTCTTCTCTGAGCATCATT+AGG 0.259035 5.1:-613378 None:intergenic
CCGCCAGCTTGAAAGGTATA+TGG 0.279189 5.1:+614074 MS.gene92329:intron
ATTTCAAATGGTCGTGATTC+TGG 0.286392 5.1:+615968 MS.gene92329:CDS
CCAACCAACGTCTCAGAAAA+AGG 0.298361 5.1:+613190 MS.gene92329:CDS
TATAAACCTCTAGTTAATTC+TGG 0.304749 5.1:+615343 MS.gene92329:CDS
TGGCTCCTCCGTACAGATTA+AGG 0.307811 5.1:+614248 MS.gene92329:CDS
ACCCAACGCAAATCAAGTTT+TGG 0.325446 5.1:+613424 MS.gene92329:CDS
AACACCCTCCTTGGAGGAAC+TGG 0.338188 5.1:-615772 None:intergenic
GAGAATTGCACTGATGTAAC+TGG 0.338466 5.1:+616154 MS.gene92329:CDS
AAGACCTTAGTGAGACCATT+TGG 0.340133 5.1:-615151 None:intergenic
CGTCTTACTCTGCTTCCTTC+CGG 0.342730 5.1:+616247 MS.gene92329:CDS
TGAGACGTTGGTTGGGTTGC+TGG 0.347361 5.1:-613182 None:intergenic
ACAACCTCCGGTCCGTTTCC+AGG 0.348649 5.1:-615661 None:intergenic
GTTTACAGTATCTCACAAAA+TGG 0.349194 5.1:-614994 None:intergenic
CTCTTCTCTGAGCATCATTA+GGG 0.354899 5.1:-613377 None:intergenic
CCAGATCCGACATTCATAAT+TGG 0.363963 5.1:-616310 None:intergenic
GAGAATCTCAGTGACATAAT+TGG 0.367087 5.1:-614370 None:intergenic
GACTTGAGTCGCCGCCATTC+AGG 0.368603 5.1:-616225 None:intergenic
TTATATAGGTTCAAACTGAT+AGG 0.374562 5.1:+613705 MS.gene92329:intron
ACAGCAACAAATTGATGAAA+TGG 0.376687 5.1:+613230 MS.gene92329:CDS
AAGGAGCCTCCACGTTCATT+TGG 0.384232 5.1:-613149 None:intergenic
ACTTACAGTGATACGAAATA+AGG 0.385266 5.1:-616002 None:intergenic
ACCAAACTGGCTAGGACATA+TGG 0.389902 5.1:+614327 MS.gene92329:CDS
ATTGTTGGATGATGTTATAT+TGG 0.393371 5.1:-614130 None:intergenic
GAGATGAAGTTAATAGATAC+AGG 0.396482 5.1:+613768 MS.gene92329:CDS
GTTGTAGAGCTTGCTGTTGT+TGG 0.396518 5.1:+614291 MS.gene92329:CDS
TCCACATGCAAGGCTAGCTC+TGG 0.401494 5.1:+616378 MS.gene92329:CDS
CGGCGGTATAGTTCATGAAT+TGG 0.401952 5.1:+615940 MS.gene92329:CDS
CCAATTATGAATGTCGGATC+TGG 0.406349 5.1:+616310 MS.gene92329:CDS
ACACTTGAAGTCCTTTCAAC+TGG 0.406882 5.1:+614785 MS.gene92329:CDS
AAGTATTTCCCAAGGGCTAA+TGG 0.411663 5.1:+614411 MS.gene92329:CDS
AGAGAAGAGAGCTGAGCCTT+CGG 0.419978 5.1:+613391 MS.gene92329:CDS
GGTTGCTGGTCATCACTTGA+AGG 0.429873 5.1:-613168 None:intergenic
AGCTTTAATCCCAATGTGTT+GGG 0.429957 5.1:-614435 None:intergenic
CTCTGAGCATCATTAGGGTT+AGG 0.434546 5.1:-613372 None:intergenic
AGACCTTAGTGAGACCATTT+GGG 0.436761 5.1:-615150 None:intergenic
GCATTTGTTATACACCCATC+TGG 0.446371 5.1:-616283 None:intergenic
GACTGGAGAATGCTCTCTTA+AGG 0.451510 5.1:+613870 MS.gene92329:CDS
TCCATATGTCCTAGCCAGTT+TGG 0.452823 5.1:-614328 None:intergenic
TTCAGGATTTCATGCATTGT+AGG 0.456298 5.1:-616208 None:intergenic
GCCACTACCCATATTCCATC+TGG 0.457430 5.1:-615022 None:intergenic
AGAAAATCAAACACCCTCCT+TGG 0.457924 5.1:-615781 None:intergenic
ACCAAAACTTGATTTGCGTT+GGG 0.459278 5.1:-613425 None:intergenic
TAATGAAATTAGATGCCATT+AGG 0.460205 5.1:-616466 None:intergenic
TGACATTTCCCCAGCGGTAG+TGG 0.460740 5.1:-613828 None:intergenic
GGAGAGGCTTTCACTGTTGG+TGG 0.462617 5.1:+614228 MS.gene92329:CDS
GCAAGAACATGTATCAGAAT+AGG 0.464753 5.1:+613100 MS.gene92329:CDS
AAAACTGCAGTGATGCCTAA+TGG 0.465524 5.1:+616451 MS.gene92329:CDS
GGCTCCTCCGTACAGATTAA+GGG 0.467368 5.1:+614249 MS.gene92329:CDS
GCTAGCTTTAGTACAAGTAT+TGG 0.471031 5.1:+615607 MS.gene92329:CDS
GTAGGAGAGGCTTTCACTGT+TGG 0.472074 5.1:+614225 MS.gene92329:CDS
CGCCAGCTTGAAAGGTATAT+GGG 0.472407 5.1:+614075 MS.gene92329:intron
TGTTGGGACCATTAGCCCTT+GGG 0.476328 5.1:-614419 None:intergenic
TTGCTGTGTGTGGCGGCGAT+AGG 0.479639 5.1:-613214 None:intergenic
ATGAGGATATCTACAAGATC+AGG 0.482347 5.1:-614498 None:intergenic
GAATTGGCTCACATTTCAAA+TGG 0.482575 5.1:+615956 MS.gene92329:CDS
GCTAATGGTCCCAACACATT+GGG 0.483917 5.1:+614426 MS.gene92329:CDS
ACATTGGGATTAAAGCTTGA+GGG 0.484683 5.1:+614441 MS.gene92329:CDS
AACCAAAACTTGATTTGCGT+TGG 0.487544 5.1:-613426 None:intergenic
AGAGCTTGCTGTTGTTGGAA+TGG 0.487738 5.1:+614296 MS.gene92329:CDS
GTTCTTCTACAACACATGCT+TGG 0.487768 5.1:+615629 MS.gene92329:CDS
AAACGGCCACCAACGTTCCA+CGG 0.489293 5.1:+615431 MS.gene92329:CDS
AAATGTAGAAGTTGATGATC+AGG 0.492812 5.1:+615306 MS.gene92329:CDS
AATGGCTGAACTGACCAAAC+TGG 0.494651 5.1:+614314 MS.gene92329:CDS
TGACGATCAGCAGCTAAGAC+TGG 0.495757 5.1:+613853 MS.gene92329:CDS
AAGCTTTAATCCCAATGTGT+TGG 0.498683 5.1:-614436 None:intergenic
TTTGGCTTCCAGTTCCTCCA+AGG 0.503857 5.1:+615764 MS.gene92329:CDS
CCACTGTTAACACTTTGATC+AGG 0.506278 5.1:+616410 MS.gene92329:CDS
GTCATGACCATTACAACCTC+CGG 0.508691 5.1:-615673 None:intergenic
TATATAGGTTCAAACTGATA+GGG 0.512992 5.1:+613706 MS.gene92329:intron
GCTTCCAGTTCCTCCAAGGA+GGG 0.515083 5.1:+615768 MS.gene92329:CDS
GAGAGCTGAGCCTTCGGACC+GGG 0.516878 5.1:+613397 MS.gene92329:CDS
AGAGAGCTGAGCCTTCGGAC+CGG 0.520073 5.1:+613396 MS.gene92329:CDS
ACTGCATCCAGATGGAATAT+GGG 0.521116 5.1:+615015 MS.gene92329:CDS
GAAGTCCTTTCAACTGGAGT+GGG 0.523037 5.1:+614791 MS.gene92329:CDS
ATTGAATCTTGCGGAAAGGT+TGG 0.528383 5.1:+615582 MS.gene92329:CDS
AACGGCCACCAACGTTCCAC+GGG 0.530317 5.1:+615432 MS.gene92329:CDS
GGGTCTTAATGAGAAGCAAA+GGG 0.531547 5.1:+614269 MS.gene92329:CDS
AACAACATGAGTGCTATTGT+TGG 0.531872 5.1:-614145 None:intergenic
TATGTCACTGAGATTCTCAA+TGG 0.532052 5.1:+614375 MS.gene92329:CDS
TGGACAAAGGTACCTGGAAA+CGG 0.533786 5.1:+615649 MS.gene92329:CDS
AAATCAAACACCCTCCTTGG+AGG 0.534430 5.1:-615778 None:intergenic
AGGGTCTTAATGAGAAGCAA+AGG 0.534523 5.1:+614268 MS.gene92329:CDS
TGCATTGTAGGTATTTGCAC+AGG 0.535629 5.1:-616196 None:intergenic
ATTAGTACTTACCACTTGCA+AGG 0.535950 5.1:-614831 None:intergenic
CGGGAATCTGCAGGATCTGT+CGG 0.536557 5.1:-614105 None:intergenic
GATTTGCGTTGGGTCTAACC+CGG 0.536665 5.1:-613415 None:intergenic
TTGATTGAGACACTCCCAAA+TGG 0.537043 5.1:+615136 MS.gene92329:CDS
GAATGCTCTCTTAAGGAAAG+AGG 0.538971 5.1:+613877 MS.gene92329:CDS
CGAGTAGGAACATGTGGGGA+GGG 0.539762 5.1:-614965 None:intergenic
GTGTTGGGACCATTAGCCCT+TGG 0.540053 5.1:-614420 None:intergenic
TGTGGAAGTTCCACTACCGC+TGG 0.541115 5.1:+613818 MS.gene92329:CDS
GGCTAATGGTCCCAACACAT+TGG 0.544303 5.1:+614425 MS.gene92329:CDS
CATGCTTGGACAAAGGTACC+TGG 0.544698 5.1:+615643 MS.gene92329:CDS
TGAAGTCCTTTCAACTGGAG+TGG 0.545864 5.1:+614790 MS.gene92329:CDS
AAACGGACCGGAGGTTGTAA+TGG 0.547300 5.1:+615666 MS.gene92329:CDS
CATCAATTTGTTGCTGTGTG+TGG 0.548832 5.1:-613224 None:intergenic
ATTATGAATGTCGGATCTGG+AGG 0.550174 5.1:+616313 MS.gene92329:CDS
GGCTTCCAGTTCCTCCAAGG+AGG 0.551888 5.1:+615767 MS.gene92329:CDS
TACCTCCCACTCCAGTTGAA+AGG 0.552733 5.1:-614796 None:intergenic
GATGGGTGTATAACAAATGC+AGG 0.554349 5.1:+616286 MS.gene92329:CDS
TACTGTAAACTGCATCCAGA+TGG 0.557363 5.1:+615007 MS.gene92329:CDS
TACAACACATGCTTGGACAA+AGG 0.558020 5.1:+615636 MS.gene92329:CDS
CACATTGGGATTAAAGCTTG+AGG 0.558168 5.1:+614440 MS.gene92329:CDS
GCACTTAGCACTACACTGAC+GGG 0.561320 5.1:-615730 None:intergenic
TTGGGTCTAACCCGGTCCGA+AGG 0.562141 5.1:-613407 None:intergenic
AGCACTTAGCACTACACTGA+CGG 0.563954 5.1:-615731 None:intergenic
CAGTGGGATATTCTCTCCGC+CGG 0.564704 5.1:+615920 MS.gene92329:intron
CTGAACTGACCAAACTGGCT+AGG 0.569467 5.1:+614319 MS.gene92329:CDS
GAAAAGTATATTGAATCTTG+CGG 0.569980 5.1:+615573 MS.gene92329:CDS
GTGGAAGTTCCACTACCGCT+GGG 0.571737 5.1:+613819 MS.gene92329:CDS
AATTGGTGGGAAGCCAAAGA+GGG 0.573774 5.1:-614353 None:intergenic
ATATTGGCACGGGAATCTGC+AGG 0.573994 5.1:-614114 None:intergenic
GTTACCACATGCAACGTATG+TGG 0.576120 5.1:+613800 MS.gene92329:CDS
AATCTCAGTGACATAATTGG+TGG 0.578666 5.1:-614367 None:intergenic
GGAGTTGCTGGTGGTAACAA+TGG 0.581415 5.1:+614186 MS.gene92329:CDS
ATTAAGACCCTTAATCTGTA+CGG 0.581991 5.1:-614256 None:intergenic
TGATCTTGTAGATATCCTCA+TGG 0.582197 5.1:+614500 MS.gene92329:CDS
GGATGATGTTATATTGGCAC+GGG 0.584066 5.1:-614124 None:intergenic
GCAGGACCAATTATGAATGT+CGG 0.584913 5.1:+616304 MS.gene92329:CDS
ACAACCAGAGGGTCTTCTGT+TGG 0.585992 5.1:-615113 None:intergenic
ATGTTCCCGTGGAACGTTGG+TGG 0.586357 5.1:-615437 None:intergenic
AGAACCAACAGAAGACCCTC+TGG 0.588399 5.1:+615109 MS.gene92329:CDS
AACGTTCCACGGGAACATCA+TGG 0.588608 5.1:+615442 MS.gene92329:CDS
GAGTAGGAACATGTGGGGAG+GGG 0.590439 5.1:-614964 None:intergenic
TTGATTCTATTCCACATGCA+AGG 0.590966 5.1:+616368 MS.gene92329:CDS
GAACAGGCGCACTTCACTCA+AGG 0.594716 5.1:+613452 MS.gene92329:CDS
CAATTTGTTGCTGTGTGTGG+CGG 0.596318 5.1:-613221 None:intergenic
GTATATTGAATCTTGCGGAA+AGG 0.597531 5.1:+615578 MS.gene92329:CDS
GTCCTTTCAACTGGAGTGGG+AGG 0.597763 5.1:+614794 MS.gene92329:CDS
CCACATGCAAGGCTAGCTCT+GGG 0.598313 5.1:+616379 MS.gene92329:CDS
AATACATGAAGTATTTCCCA+AGG 0.599100 5.1:+614403 MS.gene92329:CDS
ATCTCAGTGACATAATTGGT+GGG 0.600678 5.1:-614366 None:intergenic
TGATCAGGAACACTGTGGAA+AGG 0.601274 5.1:+616425 MS.gene92329:CDS
AAAGGTACCTGGAAACGGAC+CGG 0.603908 5.1:+615654 MS.gene92329:CDS
CACTGAGATTCTCAATGGAG+AGG 0.605470 5.1:+614380 MS.gene92329:CDS
TGTTGCATTTAACAGATGCA+AGG 0.605749 5.1:-615053 None:intergenic
ACAATGCATGAAATCCTGAA+TGG 0.606726 5.1:+616211 MS.gene92329:CDS
CAATTCATGAACTATACCGC+CGG 0.609707 5.1:-615939 None:intergenic
ACTCCCAAATGGTCTCACTA+AGG 0.610306 5.1:+615147 MS.gene92329:CDS
TAATTGGTGGGAAGCCAAAG+AGG 0.613117 5.1:-614354 None:intergenic
GGAGCACACCACCGCATGGT+AGG 0.614498 5.1:+614207 MS.gene92329:CDS
ACGAGTAGGAACATGTGGGG+AGG 0.617756 5.1:-614966 None:intergenic
ACAGTGAGATTGACTCCGGA+GGG 0.618021 5.1:+615547 MS.gene92329:intron
TGTAGATATCCTCATGGATG+TGG 0.622507 5.1:+614506 MS.gene92329:CDS
ATGTACAGTGAGATTGACTC+CGG 0.623201 5.1:+615543 MS.gene92329:intron
GCAAGTCCAGAATTAACTAG+AGG 0.625489 5.1:-615349 None:intergenic
AGATGAAGTTAATAGATACA+GGG 0.626082 5.1:+613769 MS.gene92329:CDS
TCCAGATGGAATATGGGTAG+TGG 0.628113 5.1:+615021 MS.gene92329:CDS
ACACCACCGCATGGTAGGAG+AGG 0.632377 5.1:+614212 MS.gene92329:CDS
CCCAGAGCTAGCCTTGCATG+TGG 0.637254 5.1:-616379 None:intergenic
TGGGATATTCTCTCCGCCGG+CGG 0.644828 5.1:+615923 MS.gene92329:CDS
ATTGGTGGGAAGCCAAAGAG+GGG 0.648389 5.1:-614352 None:intergenic
TGGATGATGTTATATTGGCA+CGG 0.648641 5.1:-614125 None:intergenic
TTATGAATGTCGGATCTGGA+GGG 0.649910 5.1:+616314 MS.gene92329:CDS
AAGCCTCTCCTACCATGCGG+TGG 0.649944 5.1:-614215 None:intergenic
CAATGGAGCACACCACCGCA+TGG 0.649965 5.1:+614203 MS.gene92329:CDS
AGAATTGCACTGATGTAACT+GGG 0.652163 5.1:+616155 MS.gene92329:CDS
ATGATGTTCCCGTGGAACGT+TGG 0.653734 5.1:-615440 None:intergenic
TACAGTGAGATTGACTCCGG+AGG 0.655167 5.1:+615546 MS.gene92329:intron
TATATACCATGATGTTCCCG+TGG 0.660275 5.1:-615448 None:intergenic
CTACGATGGAGCCTTGCAAG+TGG 0.661414 5.1:+614820 MS.gene92329:CDS
TGTCTCAATCAAACAACCAG+AGG 0.663405 5.1:-615125 None:intergenic
TTTGACGAGTAGGAACATGT+GGG 0.667790 5.1:-614970 None:intergenic
GTCTCAATCAAACAACCAGA+GGG 0.671321 5.1:-615124 None:intergenic
ATGAAGTTAATAGATACAGG+GGG 0.672309 5.1:+613771 MS.gene92329:CDS
AAATTCAGAGCAGAGTGATG+TGG 0.672413 5.1:+616120 MS.gene92329:CDS
GGAGTGGGAGGTAACTACGA+TGG 0.675779 5.1:+614806 MS.gene92329:CDS
GATGAAGTTAATAGATACAG+GGG 0.679151 5.1:+613770 MS.gene92329:CDS
TGAGGGTTCGAAAGAATCCG+TGG 0.680929 5.1:+614458 MS.gene92329:CDS
ACTTCCACATACGTTGCATG+TGG 0.681040 5.1:-613804 None:intergenic
TGGAAGTTCCACTACCGCTG+GGG 0.684408 5.1:+613820 MS.gene92329:CDS
ATGCATGAAATCCTGAATGG+CGG 0.684425 5.1:+616214 MS.gene92329:CDS
AGAAGCAAACCAAATGAACG+TGG 0.686882 5.1:+613140 MS.gene92329:CDS
TTGACGAGTAGGAACATGTG+GGG 0.689635 5.1:-614969 None:intergenic
TTGGTGGGAAGCCAAAGAGG+GGG 0.695051 5.1:-614351 None:intergenic
ATACATGAAGTATTTCCCAA+GGG 0.695847 5.1:+614404 MS.gene92329:CDS
AGCATTAATCATGACCACCA+CGG 0.701180 5.1:-614475 None:intergenic
GGTACCTGGAAACGGACCGG+AGG 0.704720 5.1:+615657 MS.gene92329:CDS
CACTTTGATCAGGAACACTG+TGG 0.708628 5.1:+616420 MS.gene92329:CDS
AGCAAACCAAATGAACGTGG+AGG 0.709887 5.1:+613143 MS.gene92329:CDS
AAGACCCTTAATCTGTACGG+AGG 0.717527 5.1:-614253 None:intergenic
TTCATGAACTATACCGCCGG+CGG 0.721861 5.1:-615936 None:intergenic
GTCATGTGACATTTCCCCAG+CGG 0.729104 5.1:-613834 None:intergenic
AATGACTCACCACATCCATG+AGG 0.740230 5.1:-614515 None:intergenic
GGGTTCGAAAGAATCCGTGG+TGG 0.741743 5.1:+614461 MS.gene92329:CDS
TGAAGTTAATAGATACAGGG+GGG 0.744765 5.1:+613772 MS.gene92329:CDS
TGAAAGCCTCTCCTACCATG+CGG 0.748941 5.1:-614218 None:intergenic
CCTGATCAAAGTGTTAACAG+TGG 0.772385 5.1:-616410 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTTAAAAAAAAAAAAAA+AGG - chr5.1:615475-615494 None:intergenic 0.0%
!! AAATTTAATAATATAAATGG+AGG - chr5.1:614712-614731 None:intergenic 10.0%
!! AAGTTATGTAAAATTAAAAT+TGG + chr5.1:616021-616040 MS.gene92329:intron 10.0%
!! AATTTAATAATATAAATGGA+GGG - chr5.1:614711-614730 None:intergenic 10.0%
!! TTAATAATAGTTTATAATGT+TGG + chr5.1:615203-615222 MS.gene92329:intron 10.0%
!!! AATAATATTCTAAAAATACT+TGG - chr5.1:614632-614651 None:intergenic 10.0%
!!! TTTATTTTAAAAAAGAACAT+TGG + chr5.1:614654-614673 MS.gene92329:intron 10.0%
!! AAACATTAATGCAAATAATA+AGG - chr5.1:614569-614588 None:intergenic 15.0%
!! ATATATATGAATATGATCAA+TGG - chr5.1:613267-613286 None:intergenic 15.0%
!! ATATTTAATTGAATTTACAG+TGG + chr5.1:615903-615922 MS.gene92329:intron 15.0%
!! TATTTAATTGAATTTACAGT+GGG + chr5.1:615904-615923 MS.gene92329:intron 15.0%
!!! ATTAATATTATTTTAGGTGA+AGG - chr5.1:615188-615207 None:intergenic 15.0%
!! ACAAAAAAAATACATGTAGT+TGG - chr5.1:613487-613506 None:intergenic 20.0%
!!! AAAAGAAGCATTTAATTAAG+AGG - chr5.1:613650-613669 None:intergenic 20.0%
!!! AAATTTGTTTTCAGAATCAA+TGG + chr5.1:614726-614745 MS.gene92329:intron 20.0%
!!! AATGTTGGATTATTTTAATG+TGG + chr5.1:615218-615237 MS.gene92329:intron 20.0%
!!! CTTAATTAAATGCTTCTTTT+CGG + chr5.1:613650-613669 MS.gene92329:intron 20.0%
!!! TATGTAAGAGTTGATAATAA+TGG - chr5.1:613594-613613 None:intergenic 20.0%
!!! TGGTATTAATTTTTTGAACT+TGG + chr5.1:613318-613337 MS.gene92329:intron 20.0%
! AGATGAAGTTAATAGATACA+GGG + chr5.1:613769-613788 MS.gene92329:CDS 25.0%
! ATTGTTGGATGATGTTATAT+TGG - chr5.1:614133-614152 None:intergenic 25.0%
! GCATTAATGTTTCAAAAACT+TGG + chr5.1:614577-614596 MS.gene92329:intron 25.0%
! TATAAACCTCTAGTTAATTC+TGG + chr5.1:615343-615362 MS.gene92329:CDS 25.0%
! TATATAGGTTCAAACTGATA+GGG + chr5.1:613706-613725 MS.gene92329:intron 25.0%
! TTATATAGGTTCAAACTGAT+AGG + chr5.1:613705-613724 MS.gene92329:intron 25.0%
!! GAAAAGTATATTGAATCTTG+CGG + chr5.1:615573-615592 MS.gene92329:CDS 25.0%
!!! ACTTGCTCTATATTTTTGTT+TGG + chr5.1:613298-613317 MS.gene92329:intron 25.0%
!!! GTTGTCTTGTTTTTTTATAC+AGG + chr5.1:615270-615289 MS.gene92329:intron 25.0%
!!! GTTTTTTTATACAGGTTACA+TGG + chr5.1:615278-615297 MS.gene92329:intron 25.0%
!!! TGATCGTCTTGTTTTTAAAA+CGG + chr5.1:615414-615433 MS.gene92329:CDS 25.0%
AATTGATGAAATGGATACGT+AGG + chr5.1:613239-613258 MS.gene92329:intron 30.0%
ACAGCAACAAATTGATGAAA+TGG + chr5.1:613230-613249 MS.gene92329:CDS 30.0%
ACTTACAGTGATACGAAATA+AGG - chr5.1:616005-616024 None:intergenic 30.0%
ATGAAGTTAATAGATACAGG+GGG + chr5.1:613771-613790 MS.gene92329:CDS 30.0%
ATTAAGACCCTTAATCTGTA+CGG - chr5.1:614259-614278 None:intergenic 30.0%
GAGATGAAGTTAATAGATAC+AGG + chr5.1:613768-613787 MS.gene92329:CDS 30.0%
GATGAAGTTAATAGATACAG+GGG + chr5.1:613770-613789 MS.gene92329:CDS 30.0%
GTTTACAGTATCTCACAAAA+TGG - chr5.1:614997-615016 None:intergenic 30.0%
! AATACATGAAGTATTTCCCA+AGG + chr5.1:614403-614422 MS.gene92329:CDS 30.0%
! AATATACTTTTCTTTCCCTC+CGG - chr5.1:615565-615584 None:intergenic 30.0%
! AATTAAATGCTTCTTTTCGG+CGG + chr5.1:613653-613672 MS.gene92329:intron 30.0%
! ATACATGAAGTATTTCCCAA+GGG + chr5.1:614404-614423 MS.gene92329:CDS 30.0%
! CAATGGTCAAATATGTTTTG+CGG + chr5.1:614743-614762 MS.gene92329:CDS 30.0%
! TAATAAGGCTTAGAAAGTGT+TGG - chr5.1:614554-614573 None:intergenic 30.0%
!! AAATGTAGAAGTTGATGATC+AGG + chr5.1:615306-615325 MS.gene92329:CDS 30.0%
!!! TGTATTTTTTTTGTCGTTGC+AGG + chr5.1:613493-613512 MS.gene92329:intron 30.0%
AATCTCAGTGACATAATTGG+TGG - chr5.1:614370-614389 None:intergenic 35.0%
ACAATGCATGAAATCCTGAA+TGG + chr5.1:616211-616230 MS.gene92329:CDS 35.0%
ACATTGGGATTAAAGCTTGA+GGG + chr5.1:614441-614460 MS.gene92329:CDS 35.0%
ATCTCAGTGACATAATTGGT+GGG - chr5.1:614369-614388 None:intergenic 35.0%
ATCTGGAAAAATAGCGAATC+CGG - chr5.1:616269-616288 None:intergenic 35.0%
ATGAGGATATCTACAAGATC+AGG - chr5.1:614501-614520 None:intergenic 35.0%
ATTAGTACTTACCACTTGCA+AGG - chr5.1:614834-614853 None:intergenic 35.0%
ATTTCAAATGGTCGTGATTC+TGG + chr5.1:615968-615987 MS.gene92329:CDS 35.0%
GAAAATCGTAACAAACGATG+AGG - chr5.1:613905-613924 None:intergenic 35.0%
GAGAATCTCAGTGACATAAT+TGG - chr5.1:614373-614392 None:intergenic 35.0%
GCAAGAACATGTATCAGAAT+AGG + chr5.1:613100-613119 MS.gene92329:CDS 35.0%
GTATATTGAATCTTGCGGAA+AGG + chr5.1:615578-615597 MS.gene92329:CDS 35.0%
TATGTCACTGAGATTCTCAA+TGG + chr5.1:614375-614394 MS.gene92329:CDS 35.0%
TGAAGTTAATAGATACAGGG+GGG + chr5.1:613772-613791 MS.gene92329:CDS 35.0%
TGATCTTGTAGATATCCTCA+TGG + chr5.1:614500-614519 MS.gene92329:CDS 35.0%
TGGATGATGTTATATTGGCA+CGG - chr5.1:614128-614147 None:intergenic 35.0%
TGTTGCATTTAACAGATGCA+AGG - chr5.1:615056-615075 None:intergenic 35.0%
TTCAGGATTTCATGCATTGT+AGG - chr5.1:616211-616230 None:intergenic 35.0%
TTGATTCTATTCCACATGCA+AGG + chr5.1:616368-616387 MS.gene92329:CDS 35.0%
! AACAACATGAGTGCTATTGT+TGG - chr5.1:614148-614167 None:intergenic 35.0%
! AAGCTTTAATCCCAATGTGT+TGG - chr5.1:614439-614458 None:intergenic 35.0%
! AGAATTGCACTGATGTAACT+GGG + chr5.1:616155-616174 MS.gene92329:CDS 35.0%
! AGCTTTAATCCCAATGTGTT+GGG - chr5.1:614438-614457 None:intergenic 35.0%
! GAATTGGCTCACATTTCAAA+TGG + chr5.1:615956-615975 MS.gene92329:CDS 35.0%
!! AACCAAAACTTGATTTGCGT+TGG - chr5.1:613429-613448 None:intergenic 35.0%
!! ACCAAAACTTGATTTGCGTT+GGG - chr5.1:613428-613447 None:intergenic 35.0%
!! GCTAGCTTTAGTACAAGTAT+TGG + chr5.1:615607-615626 MS.gene92329:CDS 35.0%
!! GTTAATTCTGGACTTGCTTT+TGG + chr5.1:615355-615374 MS.gene92329:CDS 35.0%
!! TAAGTGCTGCTACTTCTTTT+TGG + chr5.1:615746-615765 MS.gene92329:CDS 35.0%
!! TTCTTGCTGTGATTTTGATG+TGG - chr5.1:613087-613106 None:intergenic 35.0%
!! TTTTAGGTGAAGGTGAAATG+TGG - chr5.1:615178-615197 None:intergenic 35.0%
!!! ACAAAATGGTTTTGACGAGT+AGG - chr5.1:614983-615002 None:intergenic 35.0%
!!! GATTCGCTATTTTTCCAGAT+GGG + chr5.1:616269-616288 MS.gene92329:CDS 35.0%
!!! TCAAGTTTTGGTTTCAGAAC+AGG + chr5.1:613436-613455 MS.gene92329:CDS 35.0%
AAAAAAACCGCCAGCTTGAA+AGG + chr5.1:614067-614086 MS.gene92329:intron 40.0%
AAAACTGCAGTGATGCCTAA+TGG + chr5.1:616451-616470 MS.gene92329:CDS 40.0%
AAAACTTGGACCAAACCATC+CGG + chr5.1:614591-614610 MS.gene92329:intron 40.0%
AAATTCAGAGCAGAGTGATG+TGG + chr5.1:616120-616139 MS.gene92329:CDS 40.0%
AACTGCATCCAGATGGAATA+TGG + chr5.1:615014-615033 MS.gene92329:CDS 40.0%
AAGACCTTAGTGAGACCATT+TGG - chr5.1:615154-615173 None:intergenic 40.0%
AAGTATTTCCCAAGGGCTAA+TGG + chr5.1:614411-614430 MS.gene92329:CDS 40.0%
ACACTTGAAGTCCTTTCAAC+TGG + chr5.1:614785-614804 MS.gene92329:CDS 40.0%
ACTGCATCCAGATGGAATAT+GGG + chr5.1:615015-615034 MS.gene92329:CDS 40.0%
AGAAAATCAAACACCCTCCT+TGG - chr5.1:615784-615803 None:intergenic 40.0%
AGAAGCAAACCAAATGAACG+TGG + chr5.1:613140-613159 MS.gene92329:CDS 40.0%
AGACCTTAGTGAGACCATTT+GGG - chr5.1:615153-615172 None:intergenic 40.0%
AGCATTAATCATGACCACCA+CGG - chr5.1:614478-614497 None:intergenic 40.0%
AGGGTCTTAATGAGAAGCAA+AGG + chr5.1:614268-614287 MS.gene92329:CDS 40.0%
ATGCATGAAATCCTGAATGG+CGG + chr5.1:616214-616233 MS.gene92329:CDS 40.0%
ATTATGAATGTCGGATCTGG+AGG + chr5.1:616313-616332 MS.gene92329:CDS 40.0%
ATTGAATCTTGCGGAAAGGT+TGG + chr5.1:615582-615601 MS.gene92329:CDS 40.0%
CAATTCATGAACTATACCGC+CGG - chr5.1:615942-615961 None:intergenic 40.0%
CACAACGAATGCAATATGCA+TGG - chr5.1:613533-613552 None:intergenic 40.0%
CACATTGGGATTAAAGCTTG+AGG + chr5.1:614440-614459 MS.gene92329:CDS 40.0%
CATGCATATTGCATTCGTTG+TGG + chr5.1:613531-613550 MS.gene92329:intron 40.0%
CCAATTATGAATGTCGGATC+TGG + chr5.1:616310-616329 MS.gene92329:CDS 40.0%
CCACTGTTAACACTTTGATC+AGG + chr5.1:616410-616429 MS.gene92329:CDS 40.0%
CCAGATCCGACATTCATAAT+TGG - chr5.1:616313-616332 None:intergenic 40.0%
CTCTTCTCTGAGCATCATTA+GGG - chr5.1:613380-613399 None:intergenic 40.0%
GAATGCAATATGCATGGCAA+CGG - chr5.1:613527-613546 None:intergenic 40.0%
GAATGCTCTCTTAAGGAAAG+AGG + chr5.1:613877-613896 MS.gene92329:CDS 40.0%
GATGGGTGTATAACAAATGC+AGG + chr5.1:616286-616305 MS.gene92329:CDS 40.0%
GCAAGTCCAGAATTAACTAG+AGG - chr5.1:615352-615371 None:intergenic 40.0%
GCAGGACCAATTATGAATGT+CGG + chr5.1:616304-616323 MS.gene92329:CDS 40.0%
GGATGATGTTATATTGGCAC+GGG - chr5.1:614127-614146 None:intergenic 40.0%
GGGTCTTAATGAGAAGCAAA+GGG + chr5.1:614269-614288 MS.gene92329:CDS 40.0%
GTCTCAATCAAACAACCAGA+GGG - chr5.1:615127-615146 None:intergenic 40.0%
GTTCTTCTACAACACATGCT+TGG + chr5.1:615629-615648 MS.gene92329:CDS 40.0%
TACAACACATGCTTGGACAA+AGG + chr5.1:615636-615655 MS.gene92329:CDS 40.0%
TACTGTAAACTGCATCCAGA+TGG + chr5.1:615007-615026 MS.gene92329:CDS 40.0%
TATATACCATGATGTTCCCG+TGG - chr5.1:615451-615470 None:intergenic 40.0%
TCTCTTCTCTGAGCATCATT+AGG - chr5.1:613381-613400 None:intergenic 40.0%
TGCATTGTAGGTATTTGCAC+AGG - chr5.1:616199-616218 None:intergenic 40.0%
TGTAGATATCCTCATGGATG+TGG + chr5.1:614506-614525 MS.gene92329:CDS 40.0%
TGTCTCAATCAAACAACCAG+AGG - chr5.1:615128-615147 None:intergenic 40.0%
TTATGAATGTCGGATCTGGA+GGG + chr5.1:616314-616333 MS.gene92329:CDS 40.0%
TTGATTGAGACACTCCCAAA+TGG + chr5.1:615136-615155 MS.gene92329:CDS 40.0%
TTGGTTCAACCTGATTGAAC+CGG - chr5.1:614613-614632 None:intergenic 40.0%
TTTGACGAGTAGGAACATGT+GGG - chr5.1:614973-614992 None:intergenic 40.0%
! ACCCAACGCAAATCAAGTTT+TGG + chr5.1:613424-613443 MS.gene92329:CDS 40.0%
! ATGTACAGTGAGATTGACTC+CGG + chr5.1:615543-615562 MS.gene92329:intron 40.0%
! CCTGATCAAAGTGTTAACAG+TGG - chr5.1:616413-616432 None:intergenic 40.0%
! GAGAATTGCACTGATGTAAC+TGG + chr5.1:616154-616173 MS.gene92329:CDS 40.0%
! TTTTGACGAGTAGGAACATG+TGG - chr5.1:614974-614993 None:intergenic 40.0%
!! CATCAATTTGTTGCTGTGTG+TGG - chr5.1:613227-613246 None:intergenic 40.0%
!! GCATTTGTTATACACCCATC+TGG - chr5.1:616286-616305 None:intergenic 40.0%
!! GCTCTGAATTTTCACTCTAC+AGG - chr5.1:616111-616130 None:intergenic 40.0%
!!! CTTTTTCTGAGACGTTGGTT+GGG - chr5.1:613192-613211 None:intergenic 40.0%
!!! GGATTCGCTATTTTTCCAGA+TGG + chr5.1:616268-616287 MS.gene92329:CDS 40.0%
AAATCAAACACCCTCCTTGG+AGG - chr5.1:615781-615800 None:intergenic 45.0%
AAGACCCTTAATCTGTACGG+AGG - chr5.1:614256-614275 None:intergenic 45.0%
AATGACTCACCACATCCATG+AGG - chr5.1:614518-614537 None:intergenic 45.0%
AATGGCTGAACTGACCAAAC+TGG + chr5.1:614314-614333 MS.gene92329:CDS 45.0%
AATTGGTGGGAAGCCAAAGA+GGG - chr5.1:614356-614375 None:intergenic 45.0%
ACCAAACTGGCTAGGACATA+TGG + chr5.1:614327-614346 MS.gene92329:CDS 45.0%
ACTCCCAAATGGTCTCACTA+AGG + chr5.1:615147-615166 MS.gene92329:CDS 45.0%
ACTTCCACATACGTTGCATG+TGG - chr5.1:613807-613826 None:intergenic 45.0%
AGCAAACCAAATGAACGTGG+AGG + chr5.1:613143-613162 MS.gene92329:CDS 45.0%
AGCACTTAGCACTACACTGA+CGG - chr5.1:615734-615753 None:intergenic 45.0%
CACTGAGATTCTCAATGGAG+AGG + chr5.1:614380-614399 MS.gene92329:CDS 45.0%
CACTTTGATCAGGAACACTG+TGG + chr5.1:616420-616439 MS.gene92329:CDS 45.0%
CCAACCAACGTCTCAGAAAA+AGG + chr5.1:613190-613209 MS.gene92329:CDS 45.0%
CCATATACCTTTCAAGCTGG+CGG - chr5.1:614077-614096 None:intergenic 45.0%
CGGCGGTATAGTTCATGAAT+TGG + chr5.1:615940-615959 MS.gene92329:CDS 45.0%
CTCCCATATACCTTTCAAGC+TGG - chr5.1:614080-614099 None:intergenic 45.0%
CTCTGAGCATCATTAGGGTT+AGG - chr5.1:613375-613394 None:intergenic 45.0%
GAAGTCCTTTCAACTGGAGT+GGG + chr5.1:614791-614810 MS.gene92329:CDS 45.0%
GACTGGAGAATGCTCTCTTA+AGG + chr5.1:613870-613889 MS.gene92329:CDS 45.0%
GCTAATGGTCCCAACACATT+GGG + chr5.1:614426-614445 MS.gene92329:CDS 45.0%
GGAAAAATAGCGAATCCGGA+AGG - chr5.1:616265-616284 None:intergenic 45.0%
GTCATGACCATTACAACCTC+CGG - chr5.1:615676-615695 None:intergenic 45.0%
GTTACCACATGCAACGTATG+TGG + chr5.1:613800-613819 MS.gene92329:CDS 45.0%
TAATTGGTGGGAAGCCAAAG+AGG - chr5.1:614357-614376 None:intergenic 45.0%
TCCAGATGGAATATGGGTAG+TGG + chr5.1:615021-615040 MS.gene92329:CDS 45.0%
TCCATATGTCCTAGCCAGTT+TGG - chr5.1:614331-614350 None:intergenic 45.0%
TGAAGTCCTTTCAACTGGAG+TGG + chr5.1:614790-614809 MS.gene92329:CDS 45.0%
TGATCAGGAACACTGTGGAA+AGG + chr5.1:616425-616444 MS.gene92329:CDS 45.0%
TTCAACCTGATTGAACCGGA+TGG - chr5.1:614609-614628 None:intergenic 45.0%
TTGACGAGTAGGAACATGTG+GGG - chr5.1:614972-614991 None:intergenic 45.0%
! CGCCAGCTTGAAAGGTATAT+GGG + chr5.1:614075-614094 MS.gene92329:intron 45.0%
!! AGAGCTTGCTGTTGTTGGAA+TGG + chr5.1:614296-614315 MS.gene92329:CDS 45.0%
!! CAATTTGTTGCTGTGTGTGG+CGG - chr5.1:613224-613243 None:intergenic 45.0%
!! GTTGTAGAGCTTGCTGTTGT+TGG + chr5.1:614291-614310 MS.gene92329:CDS 45.0%
!! TGGACAAAGGTACCTGGAAA+CGG + chr5.1:615649-615668 MS.gene92329:CDS 45.0%
!!! AGGTCCTTTTTCTGAGACGT+TGG - chr5.1:613197-613216 None:intergenic 45.0%
!!! CCTTTTTCTGAGACGTTGGT+TGG - chr5.1:613193-613212 None:intergenic 45.0%
!!! GTTTCACGCTCTGCTGATTT+TGG + chr5.1:614165-614184 MS.gene92329:CDS 45.0%
!!! TCTGCTGATTTTGGAGTTGC+TGG + chr5.1:614174-614193 MS.gene92329:CDS 45.0%
!!! TGCTTTTGGAGCTAAACGCT+GGG + chr5.1:615369-615388 MS.gene92329:CDS 45.0%
!!! TTGCTTTTGGAGCTAAACGC+TGG + chr5.1:615368-615387 MS.gene92329:CDS 45.0%
!! AACAAATTTAATAATATAAA+TGG - chr5.1:614715-614734 None:intergenic 5.0%
!!! ACTATTATTAATATTATTTT+AGG - chr5.1:615194-615213 None:intergenic 5.0%
!!! TAACTAATTATATTTTATAT+AGG + chr5.1:613691-613710 MS.gene92329:intron 5.0%
!!! TTTTTTTTTTTTTACATAAA+AGG - chr5.1:614047-614066 None:intergenic 5.0%
AAACGGACCGGAGGTTGTAA+TGG + chr5.1:615666-615685 MS.gene92329:CDS 50.0%
AACGTTCCACGGGAACATCA+TGG + chr5.1:615442-615461 MS.gene92329:CDS 50.0%
AAGGAGCCTCCACGTTCATT+TGG - chr5.1:613152-613171 None:intergenic 50.0%
ACAACCAGAGGGTCTTCTGT+TGG - chr5.1:615116-615135 None:intergenic 50.0%
AGAACCAACAGAAGACCCTC+TGG + chr5.1:615109-615128 MS.gene92329:CDS 50.0%
AGAGAAGAGAGCTGAGCCTT+CGG + chr5.1:613391-613410 MS.gene92329:CDS 50.0%
ATATTGGCACGGGAATCTGC+AGG - chr5.1:614117-614136 None:intergenic 50.0%
ATGATGTTCCCGTGGAACGT+TGG - chr5.1:615443-615462 None:intergenic 50.0%
ATTGGTGGGAAGCCAAAGAG+GGG - chr5.1:614355-614374 None:intergenic 50.0%
CCAAACCATCCGGTTCAATC+AGG + chr5.1:614601-614620 MS.gene92329:intron 50.0%
CCTGATTGAACCGGATGGTT+TGG - chr5.1:614604-614623 None:intergenic 50.0%
CGTCTTACTCTGCTTCCTTC+CGG + chr5.1:616247-616266 MS.gene92329:CDS 50.0%
CTGAACTGACCAAACTGGCT+AGG + chr5.1:614319-614338 MS.gene92329:CDS 50.0%
GATTTGCGTTGGGTCTAACC+CGG - chr5.1:613418-613437 None:intergenic 50.0%
GCACTTAGCACTACACTGAC+GGG - chr5.1:615733-615752 None:intergenic 50.0%
GCCACTACCCATATTCCATC+TGG - chr5.1:615025-615044 None:intergenic 50.0%
GGAGTTGCTGGTGGTAACAA+TGG + chr5.1:614186-614205 MS.gene92329:CDS 50.0%
GGCTAATGGTCCCAACACAT+TGG + chr5.1:614425-614444 MS.gene92329:CDS 50.0%
GGCTCCTCCGTACAGATTAA+GGG + chr5.1:614249-614268 MS.gene92329:CDS 50.0%
GGTTGCTGGTCATCACTTGA+AGG - chr5.1:613171-613190 None:intergenic 50.0%
GTAGGAGAGGCTTTCACTGT+TGG + chr5.1:614225-614244 MS.gene92329:CDS 50.0%
GTCATGTGACATTTCCCCAG+CGG - chr5.1:613837-613856 None:intergenic 50.0%
TACCTCCCACTCCAGTTGAA+AGG - chr5.1:614799-614818 None:intergenic 50.0%
TGAAAGCCTCTCCTACCATG+CGG - chr5.1:614221-614240 None:intergenic 50.0%
TGACGATCAGCAGCTAAGAC+TGG + chr5.1:613853-613872 MS.gene92329:CDS 50.0%
TGAGGGTTCGAAAGAATCCG+TGG + chr5.1:614458-614477 MS.gene92329:CDS 50.0%
TGGCTCCTCCGTACAGATTA+AGG + chr5.1:614248-614267 MS.gene92329:CDS 50.0%
TGTTGGGACCATTAGCCCTT+GGG - chr5.1:614422-614441 None:intergenic 50.0%
TTCATGAACTATACCGCCGG+CGG - chr5.1:615939-615958 None:intergenic 50.0%
! ACAGTGAGATTGACTCCGGA+GGG + chr5.1:615547-615566 MS.gene92329:intron 50.0%
! CCGCCAGCTTGAAAGGTATA+TGG + chr5.1:614074-614093 MS.gene92329:intron 50.0%
! TACAGTGAGATTGACTCCGG+AGG + chr5.1:615546-615565 MS.gene92329:intron 50.0%
! TTTGGCTTCCAGTTCCTCCA+AGG + chr5.1:615764-615783 MS.gene92329:CDS 50.0%
!! AAAGGTACCTGGAAACGGAC+CGG + chr5.1:615654-615673 MS.gene92329:CDS 50.0%
!! CATGCTTGGACAAAGGTACC+TGG + chr5.1:615643-615662 MS.gene92329:CDS 50.0%
!!! GCTGATTTTGGAGTTGCTGG+TGG + chr5.1:614177-614196 MS.gene92329:CDS 50.0%
AAACGGCCACCAACGTTCCA+CGG + chr5.1:615431-615450 MS.gene92329:CDS 55.0%
AACACCCTCCTTGGAGGAAC+TGG - chr5.1:615775-615794 None:intergenic 55.0%
ACGAGTAGGAACATGTGGGG+AGG - chr5.1:614969-614988 None:intergenic 55.0%
ATGTTCCCGTGGAACGTTGG+TGG - chr5.1:615440-615459 None:intergenic 55.0%
CAGTGGGATATTCTCTCCGC+CGG + chr5.1:615920-615939 MS.gene92329:intron 55.0%
CGAGTAGGAACATGTGGGGA+GGG - chr5.1:614968-614987 None:intergenic 55.0%
CGGGAATCTGCAGGATCTGT+CGG - chr5.1:614108-614127 None:intergenic 55.0%
CTACGATGGAGCCTTGCAAG+TGG + chr5.1:614820-614839 MS.gene92329:CDS 55.0%
GAACAGGCGCACTTCACTCA+AGG + chr5.1:613452-613471 MS.gene92329:CDS 55.0%
GAGTAGGAACATGTGGGGAG+GGG - chr5.1:614967-614986 None:intergenic 55.0%
GCTTCCAGTTCCTCCAAGGA+GGG + chr5.1:615768-615787 MS.gene92329:CDS 55.0%
GGACATATGGACCCCCTCTT+TGG + chr5.1:614340-614359 MS.gene92329:CDS 55.0%
GGAGAGGCTTTCACTGTTGG+TGG + chr5.1:614228-614247 MS.gene92329:CDS 55.0%
GGAGTGGGAGGTAACTACGA+TGG + chr5.1:614806-614825 MS.gene92329:CDS 55.0%
GGGTTCGAAAGAATCCGTGG+TGG + chr5.1:614461-614480 MS.gene92329:CDS 55.0%
GTCCTTTCAACTGGAGTGGG+AGG + chr5.1:614794-614813 MS.gene92329:CDS 55.0%
GTGGAAGTTCCACTACCGCT+GGG + chr5.1:613819-613838 MS.gene92329:CDS 55.0%
GTGTTGGGACCATTAGCCCT+TGG - chr5.1:614423-614442 None:intergenic 55.0%
TGACATTTCCCCAGCGGTAG+TGG - chr5.1:613831-613850 None:intergenic 55.0%
TGGAAGTTCCACTACCGCTG+GGG + chr5.1:613820-613839 MS.gene92329:CDS 55.0%
TGTGGAAGTTCCACTACCGC+TGG + chr5.1:613818-613837 MS.gene92329:CDS 55.0%
TTGGTGGGAAGCCAAAGAGG+GGG - chr5.1:614354-614373 None:intergenic 55.0%
! CCACATGCAAGGCTAGCTCT+GGG + chr5.1:616379-616398 MS.gene92329:CDS 55.0%
! TCCACATGCAAGGCTAGCTC+TGG + chr5.1:616378-616397 MS.gene92329:CDS 55.0%
! TGAGACGTTGGTTGGGTTGC+TGG - chr5.1:613185-613204 None:intergenic 55.0%
AACGGCCACCAACGTTCCAC+GGG + chr5.1:615432-615451 MS.gene92329:CDS 60.0%
AAGCCTCTCCTACCATGCGG+TGG - chr5.1:614218-614237 None:intergenic 60.0%
ACAACCTCCGGTCCGTTTCC+AGG - chr5.1:615664-615683 None:intergenic 60.0%
ACACCACCGCATGGTAGGAG+AGG + chr5.1:614212-614231 MS.gene92329:CDS 60.0%
AGAGAGCTGAGCCTTCGGAC+CGG + chr5.1:613396-613415 MS.gene92329:CDS 60.0%
CAATGGAGCACACCACCGCA+TGG + chr5.1:614203-614222 MS.gene92329:CDS 60.0%
GACTTGAGTCGCCGCCATTC+AGG - chr5.1:616228-616247 None:intergenic 60.0%
GGCTTCCAGTTCCTCCAAGG+AGG + chr5.1:615767-615786 MS.gene92329:CDS 60.0%
TGGGATATTCTCTCCGCCGG+CGG + chr5.1:615923-615942 MS.gene92329:CDS 60.0%
TTGCTGTGTGTGGCGGCGAT+AGG - chr5.1:613217-613236 None:intergenic 60.0%
TTGGGTCTAACCCGGTCCGA+AGG - chr5.1:613410-613429 None:intergenic 60.0%
!! CCCAGAGCTAGCCTTGCATG+TGG - chr5.1:616382-616401 None:intergenic 60.0%
GAGAGCTGAGCCTTCGGACC+GGG + chr5.1:613397-613416 MS.gene92329:CDS 65.0%
GGAGCACACCACCGCATGGT+AGG + chr5.1:614207-614226 MS.gene92329:CDS 65.0%
GGTACCTGGAAACGGACCGG+AGG + chr5.1:615657-615676 MS.gene92329:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 613072 616480 613072 ID=MS.gene92329
chr5.1 mRNA 613072 616480 613072 ID=MS.gene92329.t1;Parent=MS.gene92329
chr5.1 exon 613072 613256 613072 ID=MS.gene92329.t1.exon1;Parent=MS.gene92329.t1
chr5.1 CDS 613072 613256 613072 ID=cds.MS.gene92329.t1;Parent=MS.gene92329.t1
chr5.1 exon 613356 613473 613356 ID=MS.gene92329.t1.exon2;Parent=MS.gene92329.t1
chr5.1 CDS 613356 613473 613356 ID=cds.MS.gene92329.t1;Parent=MS.gene92329.t1
chr5.1 exon 613713 613898 613713 ID=MS.gene92329.t1.exon3;Parent=MS.gene92329.t1
chr5.1 CDS 613713 613898 613713 ID=cds.MS.gene92329.t1;Parent=MS.gene92329.t1
chr5.1 exon 614081 614527 614081 ID=MS.gene92329.t1.exon4;Parent=MS.gene92329.t1
chr5.1 CDS 614081 614527 614081 ID=cds.MS.gene92329.t1;Parent=MS.gene92329.t1
chr5.1 exon 614740 614841 614740 ID=MS.gene92329.t1.exon5;Parent=MS.gene92329.t1
chr5.1 CDS 614740 614841 614740 ID=cds.MS.gene92329.t1;Parent=MS.gene92329.t1
chr5.1 exon 614944 615168 614944 ID=MS.gene92329.t1.exon6;Parent=MS.gene92329.t1
chr5.1 CDS 614944 615168 614944 ID=cds.MS.gene92329.t1;Parent=MS.gene92329.t1
chr5.1 exon 615292 615463 615292 ID=MS.gene92329.t1.exon7;Parent=MS.gene92329.t1
chr5.1 CDS 615292 615463 615292 ID=cds.MS.gene92329.t1;Parent=MS.gene92329.t1
chr5.1 exon 615551 615828 615551 ID=MS.gene92329.t1.exon8;Parent=MS.gene92329.t1
chr5.1 CDS 615551 615828 615551 ID=cds.MS.gene92329.t1;Parent=MS.gene92329.t1
chr5.1 exon 615923 616018 615923 ID=MS.gene92329.t1.exon9;Parent=MS.gene92329.t1
chr5.1 CDS 615923 616018 615923 ID=cds.MS.gene92329.t1;Parent=MS.gene92329.t1
chr5.1 exon 616115 616480 616115 ID=MS.gene92329.t1.exon10;Parent=MS.gene92329.t1
chr5.1 CDS 616115 616480 616115 ID=cds.MS.gene92329.t1;Parent=MS.gene92329.t1
Gene Sequence

>MS.gene92329

ATGGTGAATACTCCACATCAAAATCACAGCAAGAACATGTATCAGAATAGGATGACAAGAACAAGAGAAGAAGCAAACCAAATGAACGTGGAGGCTCCTTCAAGTGATGACCAGCAACCCAACCAACGTCTCAGAAAAAGGACCTATCGCCGCCACACACAGCAACAAATTGATGAAATGGATACGTAGGAACCATTGATCATATTCATATATATAATATATCTTTACTTGCTCTATATTTTTGTTTGGTATTAATTTTTTGAACTTGGTTTTGTCATCATCAGTTTCTTCAAGCAGTGTCCTAACCCTAATGATGCTCAGAGAAGAGAGCTGAGCCTTCGGACCGGGTTAGACCCAACGCAAATCAAGTTTTGGTTTCAGAACAGGCGCACTTCACTCAAGGTAATTTAGACCAACTACATGTATTTTTTTTGTCGTTGCAGGCATTGCGACCGTTGCCATGCATATTGCATTCGTTGTGGTGTAAATTTAATTTTTATTAGCATCAAATATTATAATCCATTATTATCAACTCTTACATATATTTCATTATCAACTCTGTTACTTTTACATATCCTCTTAATTAAATGCTTCTTTTCGGCGGTGATTTCATAATATATAACTAATTATATTTTATATAGGTTCAAACTGATAGGGAAGAGAATGAGCTATTAAAGATTGAGAATGAAAAACTTCGAGATGAAGTTAATAGATACAGGGGGGCAATTGTTACCACATGCAACGTATGTGGAAGTTCCACTACCGCTGGGGAAATGTCACATGACGATCAGCAGCTAAGACTGGAGAATGCTCTCTTAAGGAAAGAGGTACCTCATCGTTTGTTACGATTTTCAAAATAGATATGCGCTGAAAAAATATATAGAATTATCGAACAATTATGATTGACTTATATCGTGTTAGTACATTAATTAAATCATTCTTAGTTTGATCTCAAACTAAGCTTTAGTTTTTCCTTTTATGTAAAAAAAAAAAAAAAAAAAACCGCCAGCTTGAAAGGTATATGGGAGAGACGCCGACAGATCCTGCAGATTCCCGTGCCAATATAACATCATCCAACAATAGCACTCATGTTGTTTCACGCTCTGCTGATTTTGGAGTTGCTGGTGGTAACAATGGAGCACACCACCGCATGGTAGGAGAGGCTTTCACTGTTGGTGGCTCCTCCGTACAGATTAAGGGTCTTAATGAGAAGCAAAGGGTTGTAGAGCTTGCTGTTGTTGGAATGGCTGAACTGACCAAACTGGCTAGGACATATGGACCCCCTCTTTGGCTTCCCACCAATTATGTCACTGAGATTCTCAATGGAGAGGAATACATGAAGTATTTCCCAAGGGCTAATGGTCCCAACACATTGGGATTAAAGCTTGAGGGTTCGAAAGAATCCGTGGTGGTCATGATTAATGCTCCTGATCTTGTAGATATCCTCATGGATGTGGTGAGTCATTAATGTTCATGCTACCAACACTTTCTAAGCCTTATTATTTGCATTAATGTTTCAAAAACTTGGACCAAACCATCCGGTTCAATCAGGTTGAACCAAGTATTTTTAGAATATTATTCTTTTATTTTAAAAAAGAACATTGGTTTACATGCATACTAAAAATATATAAAATTTCCCTCCATTTATATTATTAAATTTGTTTTCAGAATCAATGGTCAAATATGTTTTGCGGCATTGTTTCTAAAGCAGCAACACTTGAAGTCCTTTCAACTGGAGTGGGAGGTAACTACGATGGAGCCTTGCAAGTGGTAAGTACTAATTTAATTTCTTATACTTAATTTTAAAGAATTAATTTTAATATGTATTTCATCTAATTAATCTTTTTTTTTTTTTTAAATGTTATTTTTTAGATGACAGCTGAATTTCAAGTCCCCTCCCCACATGTTCCTACTCGTCAAAACCATTTTGTGAGATACTGTAAACTGCATCCAGATGGAATATGGGTAGTGGCTGATGTGTCCTTGCATCTGTTAAATGCAACATCTGCTAGTAGTAGTAGTAGTAGTACTGCTTCAAGAACCAACAGAAGACCCTCTGGTTGTTTGATTGAGACACTCCCAAATGGTCTCACTAAGGTCTTACCACATTTCACCTTCACCTAAAATAATATTAATAATAGTTTATAATGTTGGATTATTTTAATGTGGATTAGTATAGTTAATTTTATCAATTATTTGTTGTCTTGTTTTTTTATACAGGTTACATGGATTGAAAATGTAGAAGTTGATGATCAGGTTGTGCAAAATATATATAAACCTCTAGTTAATTCTGGACTTGCTTTTGGAGCTAAACGCTGGGTAGCTACATTACATCGACAATCTGATCGTCTTGTTTTTAAAACGGCCACCAACGTTCCACGGGAACATCATGGTATATAACCTTTTTTTTTTTTTTTAAATAAAAAATATATATATAAATGACAGTTATATATTTATGTATAACATTATATATGTACAGTGAGATTGACTCCGGAGGGAAAGAAAAGTATATTGAATCTTGCGGAAAGGTTGGTAGCTAGCTTTAGTACAAGTATTGGTTCTTCTACAACACATGCTTGGACAAAGGTACCTGGAAACGGACCGGAGGTTGTAATGGTCATGACGAAAAGATATATCGATGAATCGAGCATTGACAAACCCGTCAGTGTAGTGCTAAGTGCTGCTACTTCTTTTTGGCTTCCAGTTCCTCCAAGGAGGGTGTTTGATTTTCTTCGAGATCAAAACACAAGAAAACATGTATGTATCTACTAAGACATGTCTAAAGTTCTGTAATGTGTTATTAGCACAATTAATTAATTGTTAAAGCTTACATATTTAATTGAATTTACAGTGGGATATTCTCTCCGCCGGCGGTATAGTTCATGAATTGGCTCACATTTCAAATGGTCGTGATTCTGGCAACTATGTCTCCTTATTTCGTATCACTGTAAGTTATGTAAAATTAAAATTGGTATAGTATGTATATTAGTGATTAGAACACATATTCTAACGTTCTCTTTCGCTAAATATATTTTTCCTGTAGAGTGAAAATTCAGAGCAGAGTGATGTGGTAGTACTGCAAGAGAATTGCACTGATGTAACTGGGTCATATGTAGTATATGCGCCTGTGCAAATACCTACAATGCATGAAATCCTGAATGGCGGCGACTCAAGTCGTCTTACTCTGCTTCCTTCCGGATTCGCTATTTTTCCAGATGGGTGTATAACAAATGCAGGACCAATTATGAATGTCGGATCTGGAGGGTCTCTTGTCACAGTTGCATTTCAGATTATAGTTGATTCTATTCCACATGCAAGGCTAGCTCTGGGTTCAATCACCACTGTTAACACTTTGATCAGGAACACTGTGGAAAGGATTAAAACTGCAGTGATGCCTAATGGCATCTAA
Protein sequence

>MS.gene92329.t1

MVNTPHQNHSKNMYQNRMTRTREEANQMNVEAPSSDDQQPNQRLRKRTYRRHTQQQIDEMDTFFKQCPNPNDAQRRELSLRTGLDPTQIKFWFQNRRTSLKVQTDREENELLKIENEKLRDEVNRYRGAIVTTCNVCGSSTTAGEMSHDDQQLRLENALLRKELERYMGETPTDPADSRANITSSNNSTHVVSRSADFGVAGGNNGAHHRMVGEAFTVGGSSVQIKGLNEKQRVVELAVVGMAELTKLARTYGPPLWLPTNYVTEILNGEEYMKYFPRANGPNTLGLKLEGSKESVVVMINAPDLVDILMDVNQWSNMFCGIVSKAATLEVLSTGVGGNYDGALQVMTAEFQVPSPHVPTRQNHFVRYCKLHPDGIWVVADVSLHLLNATSASSSSSSTASRTNRRPSGCLIETLPNGLTKVTWIENVEVDDQVVQNIYKPLVNSGLAFGAKRWVATLHRQSDRLVFKTATNVPREHHVRLTPEGKKSILNLAERLVASFSTSIGSSTTHAWTKVPGNGPEVVMVMTKRYIDESSIDKPVSVVLSAATSFWLPVPPRRVFDFLRDQNTRKHWDILSAGGIVHELAHISNGRDSGNYVSLFRITSENSEQSDVVVLQENCTDVTGSYVVYAPVQIPTMHEILNGGDSSRLTLLPSGFAIFPDGCITNAGPIMNVGSGGSLVTVAFQIIVDSIPHARLALGSITTVNTLIRNTVERIKTAVMPNGI