AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053383


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053383.t1 MTR_1g442860 97.196 214 6 0 1 214 1 214 2.46e-155 429
MS.gene053383.t1 MTR_1g075990 79.474 190 33 2 19 208 13 196 1.73e-91 266
MS.gene053383.t1 MTR_5g072510 80.282 142 28 0 55 196 27 168 7.40e-88 258
MS.gene053383.t1 MTR_4g094428 83.704 135 22 0 64 198 45 179 7.47e-87 256
MS.gene053383.t1 MTR_7g097030 75.385 195 47 1 1 195 1 194 1.14e-86 255
MS.gene053383.t1 MTR_1g069825 72.308 195 41 1 1 195 1 182 1.59e-84 250
MS.gene053383.t1 MTR_3g031830 70.760 171 39 2 37 196 3 173 2.96e-76 229
MS.gene053383.t1 MTR_2g092950 71.724 145 41 0 44 188 2 146 4.05e-75 224
MS.gene053383.t1 MTR_4g079930 74.436 133 34 0 56 188 20 152 1.21e-74 223
MS.gene053383.t1 MTR_2g076400 65.644 163 55 1 34 195 1 163 1.27e-73 221
MS.gene053383.t1 MTR_2g016110 72.662 139 38 0 57 195 27 165 7.42e-72 216
MS.gene053383.t1 MTR_7g115700 63.924 158 55 2 40 196 2 158 4.31e-71 214
MS.gene053383.t1 MTR_1g080210 78.378 148 32 0 42 189 17 164 5.85e-71 214
MS.gene053383.t1 MTR_8g086350 72.800 125 34 0 64 188 30 154 7.47e-58 181
MS.gene053383.t1 MTR_1g114020 78.431 51 11 0 58 108 64 114 8.06e-23 91.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053383.t1 AT2G31160 84.277 159 25 0 38 196 27 185 6.70e-88 258
MS.gene053383.t1 AT1G07090 77.551 147 28 1 51 197 22 163 9.40e-87 254
MS.gene053383.t1 AT3G23290 95.238 126 6 0 64 189 47 172 5.41e-82 243
MS.gene053383.t1 AT5G58500 80.741 135 25 1 63 196 17 151 9.47e-82 241
MS.gene053383.t1 AT5G28490 80.405 148 23 1 47 194 13 154 1.99e-75 226
MS.gene053383.t1 AT2G42610 77.344 128 29 0 61 188 21 148 7.17e-73 219
MS.gene053383.t1 AT2G42610 77.344 128 29 0 61 188 21 148 7.17e-73 219
MS.gene053383.t1 AT3G04510 75.168 149 37 0 46 194 14 162 6.17e-72 217
MS.gene053383.t1 AT1G78815 62.585 147 55 0 64 210 39 185 1.69e-66 203
MS.gene053383.t1 AT4G18610 64.138 145 51 1 45 188 17 161 5.39e-64 197
MS.gene053383.t1 AT1G16910 57.419 155 61 1 39 193 2 151 6.24e-62 191

Find 48 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCGTTACCTCGACCAATT+TGG 0.269006 1.4:+27086408 MS.gene053383:CDS
TGGATTTGGATGTCCATAAA+AGG 0.277270 1.4:-27086460 None:intergenic
GGACATGGTGCAGGTGGATT+TGG 0.320133 1.4:-27086474 None:intergenic
GACTTTACCTTCGCGAAGTT+AGG 0.323181 1.4:+27086613 MS.gene053383:CDS
GTTGTTGTTGATGTTGTTGA+TGG 0.340470 1.4:-27086267 None:intergenic
GGGTTTGTTATGTTGATCAT+AGG 0.347871 1.4:-27086165 None:intergenic
AAGCCAGAAGCTAATCCTTT+TGG 0.361490 1.4:+27086576 MS.gene053383:CDS
AGAAGAGATTGGAACACATT+TGG 0.367128 1.4:+27086315 MS.gene053383:CDS
GTGGTGGTCTATGGTTTCTT+AGG 0.368488 1.4:-27086344 None:intergenic
GGTGGTGGTGGTGGCGGCTG+AGG 0.370057 1.4:-27086693 None:intergenic
AAATTCTTGAAGAGAATTCA+TGG 0.377394 1.4:-27086103 None:intergenic
ACTTTACCTTCGCGAAGTTA+GGG 0.398859 1.4:+27086614 MS.gene053383:CDS
GTTGTAGTTGTATTATTTGA+TGG 0.403549 1.4:-27086714 None:intergenic
GCACCAAAAGGATTAGCTTC+TGG 0.418214 1.4:-27086579 None:intergenic
GGTTTGTTATGTTGATCATA+GGG 0.430093 1.4:-27086164 None:intergenic
GGTAGTCTTGATGCACTCAT+TGG 0.434097 1.4:+27086519 MS.gene053383:CDS
GTTGTATTATTTGATGGTGG+TGG 0.443442 1.4:-27086708 None:intergenic
TTATTTGATGGTGGTGGTGG+TGG 0.448714 1.4:-27086702 None:intergenic
AGAATCAAAAGAGAAGAGAT+TGG 0.459394 1.4:+27086304 MS.gene053383:CDS
CTTGTCCTCTTCGCCAAGCG+TGG 0.459831 1.4:+27086496 MS.gene053383:CDS
GGTGGTGGTGGCGGCTGAGG+CGG 0.465046 1.4:-27086690 None:intergenic
GTATTATTTGATGGTGGTGG+TGG 0.481872 1.4:-27086705 None:intergenic
TTGTCCTCTTCGCCAAGCGT+GGG 0.488923 1.4:+27086497 MS.gene053383:CDS
CGAGGTAACGCAAGAATTCA+AGG 0.492274 1.4:-27086398 None:intergenic
AGAGTGAAAGTGGTGGTCTA+TGG 0.493036 1.4:-27086353 None:intergenic
GTCATCGTCATCGATGATGA+TGG 0.500415 1.4:-27086186 None:intergenic
GTAGTCTTGATGCACTCATT+GGG 0.507513 1.4:+27086520 MS.gene053383:CDS
TGATGTTGTTGTTGTGGACG+AGG 0.512727 1.4:-27086241 None:intergenic
CGAACAGCACGAGCACCAAA+AGG 0.514487 1.4:-27086591 None:intergenic
GTTGTTGATGTTGTTGATGG+TGG 0.518465 1.4:-27086264 None:intergenic
TGGTGGTGATGTTGTTGTTG+TGG 0.519070 1.4:-27086247 None:intergenic
CACGCTTGGCGAAGAGGACA+AGG 0.527749 1.4:-27086495 None:intergenic
CTTTCACTCTCACGTTGTAG+TGG 0.531680 1.4:+27086366 MS.gene053383:CDS
GGATTTGGATGTCCATAAAA+GGG 0.532805 1.4:-27086459 None:intergenic
CATCAAGACTACCCCACGCT+TGG 0.543485 1.4:-27086509 None:intergenic
GTAGTTGTATTATTTGATGG+TGG 0.549591 1.4:-27086711 None:intergenic
AGAGGACAAGGACATGGTGC+AGG 0.565916 1.4:-27086483 None:intergenic
CTACCCCACGCTTGGCGAAG+AGG 0.567363 1.4:-27086501 None:intergenic
CTACAACGTGAGAGTGAAAG+TGG 0.580478 1.4:-27086363 None:intergenic
GGACAAGGACATGGTGCAGG+TGG 0.588264 1.4:-27086480 None:intergenic
TCATCGTCATCGATGATGAT+GGG 0.604263 1.4:-27086185 None:intergenic
TGTCCTCTTCGCCAAGCGTG+GGG 0.612011 1.4:+27086498 MS.gene053383:CDS
TGTTGTAGATGACGATGGTA+TGG 0.613461 1.4:-27086208 None:intergenic
GATTCTCAAGCTAAAGCTAG+AGG 0.620882 1.4:+27086636 MS.gene053383:CDS
TGGCGAAGAGGACAAGGACA+TGG 0.629659 1.4:-27086489 None:intergenic
GAGAATCCCTAACTTCGCGA+AGG 0.649287 1.4:-27086620 None:intergenic
GCTGATGTTGTAGATGACGA+TGG 0.727039 1.4:-27086213 None:intergenic
CAACGTGAGAGTGAAAGTGG+TGG 0.746060 1.4:-27086360 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GTTGTAGTTGTATTATTTGA+TGG - chr1.4:27086717-27086736 None:intergenic 25.0%
AGAATCAAAAGAGAAGAGAT+TGG + chr1.4:27086304-27086323 MS.gene053383:CDS 30.0%
GTAGTTGTATTATTTGATGG+TGG - chr1.4:27086714-27086733 None:intergenic 30.0%
!! GGTTTGTTATGTTGATCATA+GGG - chr1.4:27086167-27086186 None:intergenic 30.0%
!! TTTTGATTCTCGTATCTACT+TGG - chr1.4:27086294-27086313 None:intergenic 30.0%
!!! GAACCTTTGTTTTTCCAAAT+TGG - chr1.4:27086425-27086444 None:intergenic 30.0%
!!! GTTCATCATGTCTTTGTTTT+TGG - chr1.4:27086139-27086158 None:intergenic 30.0%
AGAAGAGATTGGAACACATT+TGG + chr1.4:27086315-27086334 MS.gene053383:CDS 35.0%
CGACCAATTTGGAAAAACAA+AGG + chr1.4:27086419-27086438 MS.gene053383:CDS 35.0%
GGATTTGGATGTCCATAAAA+GGG - chr1.4:27086462-27086481 None:intergenic 35.0%
TGGATTTGGATGTCCATAAA+AGG - chr1.4:27086463-27086482 None:intergenic 35.0%
! ACTCAGATTTGTCCCTTTTA+TGG + chr1.4:27086447-27086466 MS.gene053383:CDS 35.0%
! GTTGTTGTTGATGTTGTTGA+TGG - chr1.4:27086270-27086289 None:intergenic 35.0%
!! CTGCTTTTGAAGAAAATGGT+GGG + chr1.4:27086553-27086572 MS.gene053383:CDS 35.0%
!! GGGTTTGTTATGTTGATCAT+AGG - chr1.4:27086168-27086187 None:intergenic 35.0%
!! GTTGTATTATTTGATGGTGG+TGG - chr1.4:27086711-27086730 None:intergenic 35.0%
!! TTGTTTTTCCAAATTGGTCG+AGG - chr1.4:27086419-27086438 None:intergenic 35.0%
!!! AGAGCTGCTTTTGAAGAAAA+TGG + chr1.4:27086549-27086568 MS.gene053383:CDS 35.0%
ACTTTACCTTCGCGAAGTTA+GGG + chr1.4:27086614-27086633 MS.gene053383:CDS 40.0%
GATTCTCAAGCTAAAGCTAG+AGG + chr1.4:27086636-27086655 MS.gene053383:CDS 40.0%
TCATCGTCATCGATGATGAT+GGG - chr1.4:27086188-27086207 None:intergenic 40.0%
TGTTGTAGATGACGATGGTA+TGG - chr1.4:27086211-27086230 None:intergenic 40.0%
! AAGCCAGAAGCTAATCCTTT+TGG + chr1.4:27086576-27086595 MS.gene053383:CDS 40.0%
! GTAGTCTTGATGCACTCATT+GGG + chr1.4:27086520-27086539 MS.gene053383:CDS 40.0%
! GTTGTTGATGTTGTTGATGG+TGG - chr1.4:27086267-27086286 None:intergenic 40.0%
!! GTATTATTTGATGGTGGTGG+TGG - chr1.4:27086708-27086727 None:intergenic 40.0%
!!! GCTGCTTTTGAAGAAAATGG+TGG + chr1.4:27086552-27086571 MS.gene053383:CDS 40.0%
AGAGTGAAAGTGGTGGTCTA+TGG - chr1.4:27086356-27086375 None:intergenic 45.0%
CGAGGTAACGCAAGAATTCA+AGG - chr1.4:27086401-27086420 None:intergenic 45.0%
CTACAACGTGAGAGTGAAAG+TGG - chr1.4:27086366-27086385 None:intergenic 45.0%
CTTTCACTCTCACGTTGTAG+TGG + chr1.4:27086366-27086385 MS.gene053383:CDS 45.0%
GACTTTACCTTCGCGAAGTT+AGG + chr1.4:27086613-27086632 MS.gene053383:CDS 45.0%
GCTGATGTTGTAGATGACGA+TGG - chr1.4:27086216-27086235 None:intergenic 45.0%
GTCATCGTCATCGATGATGA+TGG - chr1.4:27086189-27086208 None:intergenic 45.0%
GTGGTGGTCTATGGTTTCTT+AGG - chr1.4:27086347-27086366 None:intergenic 45.0%
TTGCGTTACCTCGACCAATT+TGG + chr1.4:27086408-27086427 MS.gene053383:CDS 45.0%
! GGTAGTCTTGATGCACTCAT+TGG + chr1.4:27086519-27086538 MS.gene053383:CDS 45.0%
! TGATGTTGTTGTTGTGGACG+AGG - chr1.4:27086244-27086263 None:intergenic 45.0%
! TGGTGGTGATGTTGTTGTTG+TGG - chr1.4:27086250-27086269 None:intergenic 45.0%
!! GCACCAAAAGGATTAGCTTC+TGG - chr1.4:27086582-27086601 None:intergenic 45.0%
!! TTATTTGATGGTGGTGGTGG+TGG - chr1.4:27086705-27086724 None:intergenic 45.0%
CAACGTGAGAGTGAAAGTGG+TGG - chr1.4:27086363-27086382 None:intergenic 50.0%
GAGAATCCCTAACTTCGCGA+AGG - chr1.4:27086623-27086642 None:intergenic 50.0%
AGAGGACAAGGACATGGTGC+AGG - chr1.4:27086486-27086505 None:intergenic 55.0%
CATCAAGACTACCCCACGCT+TGG - chr1.4:27086512-27086531 None:intergenic 55.0%
CGAACAGCACGAGCACCAAA+AGG - chr1.4:27086594-27086613 None:intergenic 55.0%
TGGCGAAGAGGACAAGGACA+TGG - chr1.4:27086492-27086511 None:intergenic 55.0%
TTGTCCTCTTCGCCAAGCGT+GGG + chr1.4:27086497-27086516 MS.gene053383:CDS 55.0%
! GGACATGGTGCAGGTGGATT+TGG - chr1.4:27086477-27086496 None:intergenic 55.0%
!! TTTGATGGTGGTGGTGGTGG+CGG - chr1.4:27086702-27086721 None:intergenic 55.0%
CACGCTTGGCGAAGAGGACA+AGG - chr1.4:27086498-27086517 None:intergenic 60.0%
CTTGTCCTCTTCGCCAAGCG+TGG + chr1.4:27086496-27086515 MS.gene053383:CDS 60.0%
GGACAAGGACATGGTGCAGG+TGG - chr1.4:27086483-27086502 None:intergenic 60.0%
TGTCCTCTTCGCCAAGCGTG+GGG + chr1.4:27086498-27086517 MS.gene053383:CDS 60.0%
CTACCCCACGCTTGGCGAAG+AGG - chr1.4:27086504-27086523 None:intergenic 65.0%
!! GGTGGTGGTGGCGGCTGAGG+CGG - chr1.4:27086693-27086712 None:intergenic 75.0%
!! GGTGGTGGTGGTGGCGGCTG+AGG - chr1.4:27086696-27086715 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 27086105 27086749 27086105 ID=MS.gene053383
chr1.4 mRNA 27086105 27086749 27086105 ID=MS.gene053383.t1;Parent=MS.gene053383
chr1.4 exon 27086105 27086749 27086105 ID=MS.gene053383.t1.exon1;Parent=MS.gene053383.t1
chr1.4 CDS 27086105 27086749 27086105 ID=cds.MS.gene053383.t1;Parent=MS.gene053383.t1
Gene Sequence

>MS.gene053383

ATGAATTCTCTTCAAGAATTTGAATCATCAACCAAAAACAAAGACATGATGAACACAAACCCTATGATCAACATAACAAACCCATCATCATCGATGACGATGACCATACCATCGTCATCTACAACATCAGCATCATCCTCGTCCACAACAACAACATCACCACCATCAACAACATCAACAACAACACCAAGTAGATACGAGAATCAAAAGAGAAGAGATTGGAACACATTTGGACAATACCTAAGAAACCATAGACCACCACTTTCACTCTCACGTTGTAGTGGAGCTCATGTCCTTGAATTCTTGCGTTACCTCGACCAATTTGGAAAAACAAAGGTTCACACTCAGATTTGTCCCTTTTATGGACATCCAAATCCACCTGCACCATGTCCTTGTCCTCTTCGCCAAGCGTGGGGTAGTCTTGATGCACTCATTGGGAGACTTAGAGCTGCTTTTGAAGAAAATGGTGGGAAGCCAGAAGCTAATCCTTTTGGTGCTCGTGCTGTTCGACTTTACCTTCGCGAAGTTAGGGATTCTCAAGCTAAAGCTAGAGGAATTAGTTATGAGAAAAAGAAGCGCAAGCGTCCGCCTCAGCCGCCACCACCACCACCATCAAATAATACAACTACAACTGATCTTCACTAG

Protein sequence

>MS.gene053383.t1

MNSLQEFESSTKNKDMMNTNPMINITNPSSSMTMTIPSSSTTSASSSSTTTTSPPSTTSTTTPSRYENQKRRDWNTFGQYLRNHRPPLSLSRCSGAHVLEFLRYLDQFGKTKVHTQICPFYGHPNPPAPCPCPLRQAWGSLDALIGRLRAAFEENGGKPEANPFGARAVRLYLREVRDSQAKARGISYEKKKRKRPPQPPPPPPSNNTTTTDLH