AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene93372


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93372.t1 MTR_4g014460 95.413 218 7 1 1 218 1 215 1.12e-152 422
MS.gene93372.t1 MTR_7g070050 65.775 187 56 3 36 215 1 186 1.07e-76 229
MS.gene93372.t1 MTR_5g083270 62.941 170 58 3 42 209 39 205 3.39e-70 213
MS.gene93372.t1 MTR_3g071740 61.988 171 60 4 47 216 64 230 5.08e-69 211
MS.gene93372.t1 MTR_5g030500 53.659 164 72 2 49 208 44 207 6.91e-55 174
MS.gene93372.t1 MTR_1g028380 48.485 165 84 1 49 213 19 182 9.78e-55 173
MS.gene93372.t1 MTR_8g027805 47.879 165 85 1 49 213 18 181 4.54e-54 171
MS.gene93372.t1 MTR_1g016480 52.500 160 72 3 49 206 39 196 4.12e-53 170
MS.gene93372.t1 MTR_3g090980 43.956 182 97 2 32 213 8 184 1.37e-52 168
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93372.t1 AT2G38310 67.052 173 53 2 47 218 37 206 5.37e-73 220
MS.gene93372.t1 AT5G05440 64.815 162 53 2 45 205 41 199 2.89e-70 213
MS.gene93372.t1 AT2G40330 59.064 171 65 3 45 211 44 213 3.96e-66 203
MS.gene93372.t1 AT5G53160 48.485 165 84 1 49 213 19 182 4.28e-56 177
MS.gene93372.t1 AT1G01360 47.531 162 84 1 52 213 24 184 1.82e-55 175
MS.gene93372.t1 AT2G26040 49.398 166 82 1 43 206 16 181 1.39e-53 170
MS.gene93372.t1 AT4G01026 49.068 161 81 1 53 213 27 186 1.43e-53 171
MS.gene93372.t1 AT4G27920 46.667 165 88 0 49 213 14 178 6.78e-52 166
MS.gene93372.t1 AT4G17870 48.810 168 85 1 45 211 13 180 8.03e-52 166
MS.gene93372.t1 AT1G73000 46.199 171 82 4 43 203 31 201 3.59e-44 147
MS.gene93372.t1 AT5G46790 49.412 170 80 3 45 209 40 208 1.07e-43 146
MS.gene93372.t1 AT5G46790 49.412 170 80 3 45 209 40 208 1.07e-43 146
MS.gene93372.t1 AT5G45860 52.055 146 69 1 64 209 12 156 3.99e-42 140
MS.gene93372.t1 AT5G45870 50.676 148 71 1 64 211 12 157 1.03e-41 139
MS.gene93372.t1 AT4G18620 45.963 161 80 2 58 211 4 164 2.35e-34 120
MS.gene93372.t1 AT5G53160 50.495 101 49 1 49 149 19 118 6.57e-32 114

Find 72 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGAAGACTTTGGTGGATT+TGG 0.227133 4.1:-81620822 None:intergenic
CGTTCGGTGCTGCGTGCAGC+AGG 0.323719 4.1:-81621170 None:intergenic
GGACGACCGCCGAGCATAAT+TGG 0.334740 4.1:-81620995 None:intergenic
TGATAGTGGGCTATAGTATT+AGG 0.336898 4.1:-81620951 None:intergenic
CAGCAGGGAGGCCAGATATA+AGG 0.373259 4.1:-81621154 None:intergenic
TTAGGAATGGTGTTGGTGAC+AGG 0.377606 4.1:-81620933 None:intergenic
TGATGGAGGCGGTTGTCTCT+TGG 0.388404 4.1:-81621016 None:intergenic
TCCACAGAGGGTGACTGTTC+CGG 0.392902 4.1:+81621293 MS.gene93372:CDS
AGGGAGGTCAACCTTATATC+TGG 0.399072 4.1:+81621143 MS.gene93372:CDS
CACTGACCAAACGGCGGTGA+TGG 0.428126 4.1:-81621033 None:intergenic
ATATTGAGTGTGTGTGATAG+TGG 0.451535 4.1:-81620965 None:intergenic
GAAGACTTTGGTGGATTTGG+AGG 0.457214 4.1:-81620819 None:intergenic
CCGCCTCCATCACCGCCGTT+TGG 0.479222 4.1:+81621027 MS.gene93372:CDS
ATCGAGGATCTCAAGGCGTT+CGG 0.483659 4.1:-81621186 None:intergenic
CGTTGGTGGCGACGGTGATG+TGG 0.489155 4.1:+81621112 MS.gene93372:CDS
GCGGCGAACCACTGACCAAA+CGG 0.490188 4.1:-81621042 None:intergenic
CTATGTGGTGGATATTCCTT+CGG 0.490303 4.1:+81621334 MS.gene93372:CDS
GAGGTCGTGGTGGTTGGTGA+TGG 0.511327 4.1:-81620880 None:intergenic
AGTCTTCACTTCTCCTCCAC+AGG 0.523366 4.1:+81620835 MS.gene93372:CDS
TGTTTGTTGATACAATTGTT+CGG 0.525160 4.1:+81621381 MS.gene93372:CDS
CATCACCGCCGTTTGGTCAG+TGG 0.527329 4.1:+81621034 MS.gene93372:CDS
TATAGTATTAGGAATGGTGT+TGG 0.528924 4.1:-81620940 None:intergenic
GCAGTGGAGGTCGTGGTGGT+TGG 0.529799 4.1:-81620886 None:intergenic
AGGAGAAGTGAAGACTTTGG+TGG 0.533916 4.1:-81620828 None:intergenic
ACGGTGATGTGGGCACACTC+AGG 0.539446 4.1:+81621123 MS.gene93372:CDS
CCAAACGGCGGTGATGGAGG+CGG 0.539681 4.1:-81621027 None:intergenic
TGTAACCTCGTTGGTGGCGA+CGG 0.546094 4.1:+81621104 MS.gene93372:CDS
ATCAAGAGTTGTAACCTCGT+TGG 0.547255 4.1:+81621095 MS.gene93372:CDS
GTTGGTGATGGTCGTAGTGG+TGG 0.548645 4.1:-81620868 None:intergenic
CGGTGATGTGGGCACACTCA+GGG 0.556265 4.1:+81621124 MS.gene93372:CDS
GTTCGGTGCTGCGTGCAGCA+GGG 0.556567 4.1:-81621169 None:intergenic
CAACACTAAAACTGATGACA+TGG 0.557856 4.1:-81621217 None:intergenic
GATCCTCATCGAGGATCTCA+AGG 0.566346 4.1:-81621193 None:intergenic
TAGGAATGGTGTTGGTGACA+GGG 0.566710 4.1:-81620932 None:intergenic
AAGAGTTGTAACCTCGTTGG+TGG 0.571438 4.1:+81621098 MS.gene93372:CDS
AGGCTTGTGGTTTGTCGAAG+CGG 0.573243 4.1:-81621061 None:intergenic
GGAACAGTCACCCTCTGTGG+AGG 0.574183 4.1:-81621291 None:intergenic
TGGTGGTGTGGTTGATCCTG+TGG 0.579437 4.1:-81620851 None:intergenic
TCCTTCGGAAAATACTAAAG+AGG 0.584494 4.1:+81621349 MS.gene93372:CDS
ATCATCCGACCAATTATGCT+CGG 0.584649 4.1:+81620986 MS.gene93372:CDS
TGACCAAACGGCGGTGATGG+AGG 0.585643 4.1:-81621030 None:intergenic
ACACTTCATCCCTCCACAGA+GGG 0.594198 4.1:+81621281 MS.gene93372:CDS
CGGTTTGTGCAAGTGACTGA+AGG 0.594264 4.1:-81621409 None:intergenic
GCCGGAACAGTCACCCTCTG+TGG 0.599614 4.1:-81621294 None:intergenic
GGACTCAACTATCACCGTGC+CGG 0.603129 4.1:-81621312 None:intergenic
GAACAGTCACCCTCTGTGGA+GGG 0.606405 4.1:-81621290 None:intergenic
GACCGCCGAGCATAATTGGT+CGG 0.608055 4.1:-81620991 None:intergenic
GTGGGCTATAGTATTAGGAA+TGG 0.608376 4.1:-81620946 None:intergenic
GTTGGTGGCGACGGTGATGT+GGG 0.610157 4.1:+81621113 MS.gene93372:CDS
CGAAGGAATATCCACCACAT+AGG 0.619850 4.1:-81621333 None:intergenic
TGGAGGAGAAGTGAAGACTT+TGG 0.627794 4.1:-81620831 None:intergenic
AGAGGGTGACTGTTCCGGCA+CGG 0.639448 4.1:+81621298 MS.gene93372:CDS
GGAGGGATGAAGTGTAGTAA+CGG 0.646842 4.1:-81621273 None:intergenic
TGATGGTCGTAGTGGTGGTG+TGG 0.646910 4.1:-81620863 None:intergenic
GTGGTTGGTGATGGTCGTAG+TGG 0.657655 4.1:-81620871 None:intergenic
CGGTGCTGCGTGCAGCAGGG+AGG 0.659975 4.1:-81621166 None:intergenic
TATTGAGTGTGTGTGATAGT+GGG 0.661022 4.1:-81620964 None:intergenic
TGGTGACAGGGTTGACGAAG+AGG 0.662879 4.1:-81620920 None:intergenic
TGGTGTGGTTGATCCTGTGG+AGG 0.662950 4.1:-81620848 None:intergenic
GGTGACAGGGTTGACGAAGA+GGG 0.665804 4.1:-81620919 None:intergenic
GATGAAGTGTAGTAACGGAA+CGG 0.672049 4.1:-81621268 None:intergenic
TTTCGCAGTGGAGGTCGTGG+TGG 0.685087 4.1:-81620890 None:intergenic
ACGCCTTGAGATCCTCGATG+AGG 0.696994 4.1:+81621190 MS.gene93372:CDS
ATCCGACCAATTATGCTCGG+CGG 0.704061 4.1:+81620989 MS.gene93372:CDS
TTGCTGTGTAATGTTCTCTG+CGG 0.706407 4.1:-81621429 None:intergenic
GATAGTTGAGTCCTATGTGG+TGG 0.708119 4.1:+81621322 MS.gene93372:CDS
GGTGATAGTTGAGTCCTATG+TGG 0.715010 4.1:+81621319 MS.gene93372:CDS
TGATGTGGGCACACTCAGGG+AGG 0.725955 4.1:+81621127 MS.gene93372:CDS
CATCACCGTCGCCACCAACG+AGG 0.726271 4.1:-81621109 None:intergenic
TACACTTCATCCCTCCACAG+AGG 0.732326 4.1:+81621280 MS.gene93372:CDS
TGACATGGCGATCCTCATCG+AGG 0.742190 4.1:-81621202 None:intergenic
GCGAACCACTGACCAAACGG+CGG 0.744614 4.1:-81621039 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTCTTGATGAAATTTTTGT+AGG - chr4.1:81621084-81621103 None:intergenic 25.0%
!! TGTTTGTTGATACAATTGTT+CGG + chr4.1:81621381-81621400 MS.gene93372:CDS 25.0%
TATAGTATTAGGAATGGTGT+TGG - chr4.1:81620943-81620962 None:intergenic 30.0%
! ATGAAATTTTTGTAGGCTTG+TGG - chr4.1:81621077-81621096 None:intergenic 30.0%
ATATTGAGTGTGTGTGATAG+TGG - chr4.1:81620968-81620987 None:intergenic 35.0%
CAACACTAAAACTGATGACA+TGG - chr4.1:81621220-81621239 None:intergenic 35.0%
TATTGAGTGTGTGTGATAGT+GGG - chr4.1:81620967-81620986 None:intergenic 35.0%
TCCTTCGGAAAATACTAAAG+AGG + chr4.1:81621349-81621368 MS.gene93372:CDS 35.0%
! TCCTCTTTAGTATTTTCCGA+AGG - chr4.1:81621353-81621372 None:intergenic 35.0%
!! ATCAGTTTTAGTGTTGTTGG+TGG + chr4.1:81621224-81621243 MS.gene93372:CDS 35.0%
!! GTCATCAGTTTTAGTGTTGT+TGG + chr4.1:81621221-81621240 MS.gene93372:CDS 35.0%
!! TGATAGTGGGCTATAGTATT+AGG - chr4.1:81620954-81620973 None:intergenic 35.0%
ATCAAGAGTTGTAACCTCGT+TGG + chr4.1:81621095-81621114 MS.gene93372:CDS 40.0%
ATCATCCGACCAATTATGCT+CGG + chr4.1:81620986-81621005 MS.gene93372:CDS 40.0%
TTGCTGTGTAATGTTCTCTG+CGG - chr4.1:81621432-81621451 None:intergenic 40.0%
! GATGAAGTGTAGTAACGGAA+CGG - chr4.1:81621271-81621290 None:intergenic 40.0%
! GTGGGCTATAGTATTAGGAA+TGG - chr4.1:81620949-81620968 None:intergenic 40.0%
!! AGTGAAGACTTTGGTGGATT+TGG - chr4.1:81620825-81620844 None:intergenic 40.0%
!! CTATGTGGTGGATATTCCTT+CGG + chr4.1:81621334-81621353 MS.gene93372:CDS 40.0%
AAGAGTTGTAACCTCGTTGG+TGG + chr4.1:81621098-81621117 MS.gene93372:CDS 45.0%
AGGAGAAGTGAAGACTTTGG+TGG - chr4.1:81620831-81620850 None:intergenic 45.0%
AGGGAGGTCAACCTTATATC+TGG + chr4.1:81621143-81621162 MS.gene93372:CDS 45.0%
CGAAGGAATATCCACCACAT+AGG - chr4.1:81621336-81621355 None:intergenic 45.0%
GATAGTTGAGTCCTATGTGG+TGG + chr4.1:81621322-81621341 MS.gene93372:CDS 45.0%
GGAGGGATGAAGTGTAGTAA+CGG - chr4.1:81621276-81621295 None:intergenic 45.0%
GGTGATAGTTGAGTCCTATG+TGG + chr4.1:81621319-81621338 MS.gene93372:CDS 45.0%
TGGAGGAGAAGTGAAGACTT+TGG - chr4.1:81620834-81620853 None:intergenic 45.0%
!! GAAGACTTTGGTGGATTTGG+AGG - chr4.1:81620822-81620841 None:intergenic 45.0%
!! TAGGAATGGTGTTGGTGACA+GGG - chr4.1:81620935-81620954 None:intergenic 45.0%
!! TTAGGAATGGTGTTGGTGAC+AGG - chr4.1:81620936-81620955 None:intergenic 45.0%
ACACTTCATCCCTCCACAGA+GGG + chr4.1:81621281-81621300 MS.gene93372:CDS 50.0%
AGTCTTCACTTCTCCTCCAC+AGG + chr4.1:81620835-81620854 MS.gene93372:CDS 50.0%
ATCCGACCAATTATGCTCGG+CGG + chr4.1:81620989-81621008 MS.gene93372:CDS 50.0%
CGGTTTGTGCAAGTGACTGA+AGG - chr4.1:81621412-81621431 None:intergenic 50.0%
GATCCTCATCGAGGATCTCA+AGG - chr4.1:81621196-81621215 None:intergenic 50.0%
TACACTTCATCCCTCCACAG+AGG + chr4.1:81621280-81621299 MS.gene93372:CDS 50.0%
! AGGCTTGTGGTTTGTCGAAG+CGG - chr4.1:81621064-81621083 None:intergenic 50.0%
! ATCGAGGATCTCAAGGCGTT+CGG - chr4.1:81621189-81621208 None:intergenic 50.0%
!!! AGAGGGTGTTGTTTTCGCAG+TGG - chr4.1:81620905-81620924 None:intergenic 50.0%
ACGCCTTGAGATCCTCGATG+AGG + chr4.1:81621190-81621209 MS.gene93372:CDS 55.0%
CAGCAGGGAGGCCAGATATA+AGG - chr4.1:81621157-81621176 None:intergenic 55.0%
GAACAGTCACCCTCTGTGGA+GGG - chr4.1:81621293-81621312 None:intergenic 55.0%
GACCGCCGAGCATAATTGGT+CGG - chr4.1:81620994-81621013 None:intergenic 55.0%
GGACTCAACTATCACCGTGC+CGG - chr4.1:81621315-81621334 None:intergenic 55.0%
GGTGACAGGGTTGACGAAGA+GGG - chr4.1:81620922-81620941 None:intergenic 55.0%
TCCACAGAGGGTGACTGTTC+CGG + chr4.1:81621293-81621312 MS.gene93372:CDS 55.0%
TGACATGGCGATCCTCATCG+AGG - chr4.1:81621205-81621224 None:intergenic 55.0%
TGATGGAGGCGGTTGTCTCT+TGG - chr4.1:81621019-81621038 None:intergenic 55.0%
TGGTGACAGGGTTGACGAAG+AGG - chr4.1:81620923-81620942 None:intergenic 55.0%
TGGTGTGGTTGATCCTGTGG+AGG - chr4.1:81620851-81620870 None:intergenic 55.0%
! TGATGGTCGTAGTGGTGGTG+TGG - chr4.1:81620866-81620885 None:intergenic 55.0%
! TGGTGGTGTGGTTGATCCTG+TGG - chr4.1:81620854-81620873 None:intergenic 55.0%
! TGTTTTCGCAGTGGAGGTCG+TGG - chr4.1:81620896-81620915 None:intergenic 55.0%
!! GTGGTTGGTGATGGTCGTAG+TGG - chr4.1:81620874-81620893 None:intergenic 55.0%
!! GTTGGTGATGGTCGTAGTGG+TGG - chr4.1:81620871-81620890 None:intergenic 55.0%
!! TGTAACCTCGTTGGTGGCGA+CGG + chr4.1:81621104-81621123 MS.gene93372:CDS 55.0%
!!! GGGTGTTGTTTTCGCAGTGG+AGG - chr4.1:81620902-81620921 None:intergenic 55.0%
ACGGTGATGTGGGCACACTC+AGG + chr4.1:81621123-81621142 MS.gene93372:CDS 60.0%
AGAGGGTGACTGTTCCGGCA+CGG + chr4.1:81621298-81621317 MS.gene93372:CDS 60.0%
CACTGACCAAACGGCGGTGA+TGG - chr4.1:81621036-81621055 None:intergenic 60.0%
CATCACCGCCGTTTGGTCAG+TGG + chr4.1:81621034-81621053 MS.gene93372:CDS 60.0%
CGGTGATGTGGGCACACTCA+GGG + chr4.1:81621124-81621143 MS.gene93372:CDS 60.0%
GCGAACCACTGACCAAACGG+CGG - chr4.1:81621042-81621061 None:intergenic 60.0%
GCGGCGAACCACTGACCAAA+CGG - chr4.1:81621045-81621064 None:intergenic 60.0%
GGAACAGTCACCCTCTGTGG+AGG - chr4.1:81621294-81621313 None:intergenic 60.0%
GGACGACCGCCGAGCATAAT+TGG - chr4.1:81620998-81621017 None:intergenic 60.0%
TGATGTGGGCACACTCAGGG+AGG + chr4.1:81621127-81621146 MS.gene93372:CDS 60.0%
TTTCGCAGTGGAGGTCGTGG+TGG - chr4.1:81620893-81620912 None:intergenic 60.0%
! TGACCAAACGGCGGTGATGG+AGG - chr4.1:81621033-81621052 None:intergenic 60.0%
!! GAGGTCGTGGTGGTTGGTGA+TGG - chr4.1:81620883-81620902 None:intergenic 60.0%
!! GTTGGTGGCGACGGTGATGT+GGG + chr4.1:81621113-81621132 MS.gene93372:CDS 60.0%
CATCACCGTCGCCACCAACG+AGG - chr4.1:81621112-81621131 None:intergenic 65.0%
GCAGTGGAGGTCGTGGTGGT+TGG - chr4.1:81620889-81620908 None:intergenic 65.0%
GCCGGAACAGTCACCCTCTG+TGG - chr4.1:81621297-81621316 None:intergenic 65.0%
!! CCAAACGGCGGTGATGGAGG+CGG - chr4.1:81621030-81621049 None:intergenic 65.0%
!! CGTTGGTGGCGACGGTGATG+TGG + chr4.1:81621112-81621131 MS.gene93372:CDS 65.0%
!! GTTCGGTGCTGCGTGCAGCA+GGG - chr4.1:81621172-81621191 None:intergenic 65.0%
CCGCCTCCATCACCGCCGTT+TGG + chr4.1:81621027-81621046 MS.gene93372:CDS 70.0%
!! CGTTCGGTGCTGCGTGCAGC+AGG - chr4.1:81621173-81621192 None:intergenic 70.0%
!! CGGTGCTGCGTGCAGCAGGG+AGG - chr4.1:81621169-81621188 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 81620816 81621472 81620816 ID=MS.gene93372
chr4.1 mRNA 81620816 81621472 81620816 ID=MS.gene93372.t1;Parent=MS.gene93372
chr4.1 exon 81620816 81621472 81620816 ID=MS.gene93372.t1.exon1;Parent=MS.gene93372.t1
chr4.1 CDS 81620816 81621472 81620816 ID=cds.MS.gene93372.t1;Parent=MS.gene93372.t1
Gene Sequence

>MS.gene93372

ATGCCTCCAAATCCACCAAAGTCTTCACTTCTCCTCCACAGGATCAACCACACCACCACTACGACCATCACCAACCACCACGACCTCCACTGCGAAAACAACACCCTCTTCGTCAACCCTGTCACCAACACCATTCCTAATACTATAGCCCACTATCACACACACTCAATATCATCCGACCAATTATGCTCGGCGGTCGTCCAAGAGACAACCGCCTCCATCACCGCCGTTTGGTCAGTGGTTCGCCGCTTCGACAAACCACAAGCCTACAAAAATTTCATCAAGAGTTGTAACCTCGTTGGTGGCGACGGTGATGTGGGCACACTCAGGGAGGTCAACCTTATATCTGGCCTCCCTGCTGCACGCAGCACCGAACGCCTTGAGATCCTCGATGAGGATCGCCATGTCATCAGTTTTAGTGTTGTTGGTGGTGATCACAAGCTTGCTAACTACCGTTCCGTTACTACACTTCATCCCTCCACAGAGGGTGACTGTTCCGGCACGGTGATAGTTGAGTCCTATGTGGTGGATATTCCTTCGGAAAATACTAAAGAGGACACACATGTGTTTGTTGATACAATTGTTCGGTGTAACCTTCAGTCACTTGCACAAACCGCAGAGAACATTACACAGCAAAACAACAAGAGTTGTTCCTGA

Protein sequence

>MS.gene93372.t1

MPPNPPKSSLLLHRINHTTTTTITNHHDLHCENNTLFVNPVTNTIPNTIAHYHTHSISSDQLCSAVVQETTASITAVWSVVRRFDKPQAYKNFIKSCNLVGGDGDVGTLREVNLISGLPAARSTERLEILDEDRHVISFSVVGGDHKLANYRSVTTLHPSTEGDCSGTVIVESYVVDIPSENTKEDTHVFVDTIVRCNLQSLAQTAENITQQNNKSCS