AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene93900

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93900.t1 MTR_4g033200 91.416 233 20 0 1 233 31 263 6.32e-158 442
MS.gene93900.t1 MTR_4g033015 91.416 233 20 0 1 233 31 263 2.02e-157 441
MS.gene93900.t1 MTR_4g033150 88.412 233 27 0 1 233 31 263 1.61e-152 429
MS.gene93900.t1 MTR_0794s0020 88.128 219 26 0 1 219 31 249 4.07e-144 403
MS.gene93900.t1 MTR_4g033085 74.678 233 53 1 1 233 31 257 2.54e-127 364
MS.gene93900.t1 MTR_4g033170 74.678 233 53 1 1 233 31 257 3.49e-127 364
MS.gene93900.t1 MTR_4g088200 73.820 233 59 1 1 233 31 261 6.26e-123 353
MS.gene93900.t1 MTR_4g088190 76.496 234 54 1 1 233 31 264 1.13e-114 333
MS.gene93900.t1 MTR_7g014570 52.137 234 102 3 1 234 31 254 1.64e-77 238
MS.gene93900.t1 MTR_7g014510 49.160 238 113 4 1 235 31 263 2.03e-73 227
MS.gene93900.t1 MTR_5g074680 47.436 234 116 4 1 233 32 259 2.02e-68 215
MS.gene93900.t1 MTR_7g024500 46.352 233 113 4 1 233 30 250 2.12e-66 209
MS.gene93900.t1 MTR_5g074600 44.872 234 122 4 1 233 31 258 2.78e-66 209
MS.gene93900.t1 MTR_1g076940 46.186 236 120 5 1 233 31 262 3.30e-65 206
MS.gene93900.t1 MTR_5g074580 45.299 234 121 4 1 233 32 259 3.34e-64 204
MS.gene93900.t1 MTR_7g024520 46.352 233 113 4 1 233 30 250 7.18e-62 197
MS.gene93900.t1 MTR_4g088345 48.291 234 112 4 1 233 31 256 7.90e-62 197
MS.gene93900.t1 MTR_5g074610 45.064 233 95 3 1 233 27 226 2.18e-60 193
MS.gene93900.t1 MTR_4g088340 47.009 234 115 4 1 233 8 233 1.45e-59 191
MS.gene93900.t1 MTR_4g079160 42.735 234 125 3 1 233 30 255 2.97e-59 191
MS.gene93900.t1 MTR_5g074540 41.453 234 112 5 1 233 31 240 8.51e-59 189
MS.gene93900.t1 MTR_1g076930 44.304 237 121 6 1 233 8 237 3.06e-58 185
MS.gene93900.t1 MTR_3g083620 36.481 233 137 6 2 233 39 261 6.03e-35 128
MS.gene93900.t1 MTR_4g038440 33.191 235 147 5 2 233 43 270 9.22e-30 112
MS.gene93900.t1 MTR_7g012090 31.020 245 155 6 2 236 40 280 1.19e-29 114
MS.gene93900.t1 MTR_0079s0070 33.191 235 147 5 2 233 9 236 3.11e-29 112
MS.gene93900.t1 MTR_4g038440 33.191 235 147 5 2 233 43 270 5.05e-29 112
MS.gene93900.t1 MTR_7g011990 31.429 245 146 7 2 236 40 272 1.55e-28 111
MS.gene93900.t1 MTR_0017s0100 32.627 236 149 5 1 233 42 270 2.64e-28 110
MS.gene93900.t1 MTR_7g011900 31.667 240 155 6 2 236 40 275 3.84e-28 110
MS.gene93900.t1 MTR_7g012020 31.224 237 154 6 2 233 39 271 4.73e-28 108
MS.gene93900.t1 MTR_7g012070 30.579 242 154 7 2 233 40 277 1.27e-27 108
MS.gene93900.t1 MTR_7g012040 30.962 239 157 6 2 236 39 273 1.29e-27 108
MS.gene93900.t1 MTR_7g012020 31.224 237 154 6 2 233 39 271 1.61e-27 108
MS.gene93900.t1 MTR_1g096310 31.405 242 155 6 2 236 40 277 5.46e-26 104
MS.gene93900.t1 MTR_1g036460 31.535 241 154 6 3 236 38 274 6.26e-26 104
MS.gene93900.t1 MTR_2g055940 30.165 242 155 8 2 236 34 268 4.10e-25 102
MS.gene93900.t1 MTR_1g036490 31.120 241 155 6 3 236 38 274 2.04e-24 100
MS.gene93900.t1 MTR_5g098170 32.510 243 150 9 1 236 33 268 7.88e-24 98.2
MS.gene93900.t1 MTR_8g068870 31.381 239 153 7 1 233 32 265 2.81e-23 96.7
MS.gene93900.t1 MTR_2g072790 32.353 238 147 6 1 233 27 255 4.16e-23 96.3
MS.gene93900.t1 MTR_3g092900 30.833 240 157 6 1 236 34 268 1.12e-21 92.4
MS.gene93900.t1 MTR_8g068690 29.832 238 158 6 1 233 45 278 1.86e-21 90.9
MS.gene93900.t1 MTR_0591s0010 30.672 238 156 6 1 233 46 279 2.95e-21 91.3
MS.gene93900.t1 MTR_8g068690 29.412 238 159 6 1 233 45 278 5.06e-21 90.9
MS.gene93900.t1 MTR_7g118320 29.412 238 159 6 1 233 31 264 9.33e-21 89.7
MS.gene93900.t1 MTR_3g096050 31.092 238 149 8 2 233 31 259 1.41e-20 87.8
MS.gene93900.t1 MTR_1g036510 30.516 213 142 5 26 236 80 288 1.63e-20 89.4
MS.gene93900.t1 MTR_1g036510 30.476 210 140 5 26 233 80 285 2.19e-20 87.8
MS.gene93900.t1 MTR_8g068750 29.832 238 158 6 1 233 46 279 2.41e-20 89.0
MS.gene93900.t1 MTR_3g096050 31.092 238 149 8 2 233 31 259 3.56e-20 88.2
MS.gene93900.t1 MTR_7g118300 29.461 241 152 6 1 233 47 277 5.78e-20 87.8
MS.gene93900.t1 MTR_3g021440 30.508 236 156 6 2 233 42 273 2.18e-19 86.3
MS.gene93900.t1 MTR_7g012020 30.476 210 137 6 2 206 39 244 2.96e-19 84.0
MS.gene93900.t1 MTR_8g068600 28.205 234 159 6 8 236 27 256 2.45e-18 82.0
MS.gene93900.t1 MTR_8g068570 28.151 238 135 7 1 233 46 252 5.95e-17 79.0
MS.gene93900.t1 MTR_3g021430 28.814 236 160 6 2 233 29 260 8.97e-17 78.6
MS.gene93900.t1 MTR_8g068620 37.864 103 62 1 131 233 10 110 5.92e-16 72.0
MS.gene93900.t1 MTR_3g096070 28.814 236 134 7 2 233 31 236 1.46e-15 75.1
MS.gene93900.t1 MTR_8g024160 31.138 167 110 3 68 233 68 230 2.47e-15 74.3
MS.gene93900.t1 MTR_6g078850 26.250 240 166 6 1 233 50 285 3.32e-15 74.7
MS.gene93900.t1 MTR_1g036510 28.415 183 125 5 26 206 80 258 3.55e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93900.t1 AT5G54160 32.353 238 154 5 1 236 34 266 1.16e-26 106
MS.gene93900.t1 AT3G53140 32.099 243 146 8 1 233 27 260 3.02e-23 96.7
MS.gene93900.t1 AT1G77520 30.924 249 146 7 1 233 44 282 1.35e-22 95.1
MS.gene93900.t1 AT4G35150 29.362 235 116 7 2 233 31 218 2.07e-21 91.3
MS.gene93900.t1 AT4G35160 33.333 243 139 9 2 233 45 275 2.24e-20 89.0
MS.gene93900.t1 AT1G33030 28.512 242 160 7 1 236 21 255 3.19e-19 84.3
MS.gene93900.t1 AT1G33030 28.395 243 161 7 1 237 21 256 7.16e-19 83.2
MS.gene93900.t1 AT1G33030 28.452 239 158 7 1 233 21 252 5.51e-18 82.0
MS.gene93900.t1 AT1G51990 26.941 219 150 6 21 235 56 268 2.15e-16 77.8
MS.gene93900.t1 AT1G63140 28.689 244 158 7 1 233 64 302 1.81e-15 74.7
MS.gene93900.t1 AT1G51990 26.829 246 158 8 3 235 32 268 1.86e-15 75.1
MS.gene93900.t1 AT1G63140 28.689 244 158 7 1 233 64 302 2.48e-15 74.7
MS.gene93900.t1 AT1G63140 28.689 244 158 7 1 233 44 282 2.63e-15 74.7
MS.gene93900.t1 AT1G77530 30.041 243 156 6 1 233 44 282 5.77e-15 73.6
MS.gene93900.t1 AT5G53810 28.279 244 160 6 1 233 40 279 6.10e-15 73.6

Find 49 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAAGAGGCGAATCATTTC+GGG 0.293856 4.4:-70294377 MS.gene93900:CDS
GCACAAGAGGCGAATCATTT+CGG 0.304099 4.4:-70294378 MS.gene93900:CDS
CTCAGTGAGCCATTTCTTAG+AGG 0.316815 4.4:+70294426 None:intergenic
GCTAATTCTGTAAGAGTCAT+TGG 0.343004 4.4:+70294682 None:intergenic
AACGAAAATTTAAAATTTGT+TGG 0.352104 4.4:-70294115 MS.gene93900:CDS
TACAGGACACTAACTTTAGA+AGG 0.365683 4.4:+70294640 None:intergenic
AGCACGATTGTTTCTGCAAT+AGG 0.368065 4.4:-70293434 MS.gene93900:CDS
TAGCCATGTGTTTGAAGGTT+TGG 0.381600 4.4:-70294257 MS.gene93900:CDS
TGTTTGTCACCATTTGTTAC+CGG 0.405515 4.4:-70294484 MS.gene93900:CDS
TGAGGTGAGGAAAAGTTTCA+TGG 0.410589 4.4:+70294185 None:intergenic
GAGTGTAGCCATGTGTTTGA+AGG 0.425506 4.4:-70294262 MS.gene93900:CDS
GTTTGAAGGTTTGGAGTCTC+TGG 0.426025 4.4:-70294248 MS.gene93900:CDS
CCACTGATGAGAAGCATTGA+AGG 0.442792 4.4:+70294517 None:intergenic
GTTGATGTTGGAGGTGGCAC+AGG 0.461040 4.4:-70294226 MS.gene93900:CDS
TGTGGCACTCCTCTAAGAAA+TGG 0.479752 4.4:-70294435 MS.gene93900:CDS
CTCAAATCAGCCGTGGAACT+AGG 0.484791 4.4:-70294736 MS.gene93900:CDS
TTGGAGTCTCTGGTTGATGT+TGG 0.495110 4.4:-70294238 MS.gene93900:CDS
GGTCAAATACTATGCATTTG+AGG 0.496242 4.4:+70294167 None:intergenic
GAGTCTCTGGTTGATGTTGG+AGG 0.503583 4.4:-70294235 MS.gene93900:CDS
GAAAATTTAAAATTTGTTGG+TGG 0.514676 4.4:-70294112 MS.gene93900:CDS
ACACTGATGAATGGCAAAGA+AGG 0.516027 4.4:-70294571 MS.gene93900:CDS
CACGGCTGATTTGAGTGCCA+TGG 0.517659 4.4:+70294744 None:intergenic
GATGTTGGAGGTGGCACAGG+AGG 0.520874 4.4:-70294223 MS.gene93900:CDS
ATCTGCTATGCCTAGTTCCA+CGG 0.523313 4.4:+70294726 None:intergenic
TGAAGCGATCCGGTAACAAA+TGG 0.526003 4.4:+70294475 None:intergenic
CGATTGTTTCTGCAATAGGT+AGG 0.534029 4.4:-70293430 None:intergenic
ACGACATGGATGAAGCGATC+CGG 0.554100 4.4:+70294465 None:intergenic
TTGAGTAAAACAGCATCTGC+AGG 0.557051 4.4:+70294067 None:intergenic
GCAAGCGCAAGAGACGATAC+AGG 0.558531 4.4:+70294623 None:intergenic
AGTCTCTTTGAGAGCACAAG+AGG 0.560449 4.4:-70294391 MS.gene93900:CDS
CCTTCAATGCTTCTCATCAG+TGG 0.573536 4.4:-70294517 MS.gene93900:CDS
GATTTGAGAATCAGCTGCCA+TGG 0.583476 4.4:+70294303 None:intergenic
TCTCTGGTTGATGTTGGAGG+TGG 0.586831 4.4:-70294232 MS.gene93900:CDS
ATCCATGTCGTCTCAACATG+TGG 0.587101 4.4:-70294453 MS.gene93900:CDS
GAGTATGTTTCAAGATGCCA+TGG 0.599550 4.4:-70294320 MS.gene93900:CDS
CATAGTATTTGACCAACCAC+AGG 0.602779 4.4:-70294158 MS.gene93900:CDS
TTGCGCTTGCTAACACACAA+TGG 0.607728 4.4:-70294610 MS.gene93900:CDS
AATACTATGCATTTGAGGTG+AGG 0.609052 4.4:+70294172 None:intergenic
GGACACTAACTTTAGAAGGG+TGG 0.621869 4.4:+70294644 None:intergenic
TGCCACATGTTGAGACGACA+TGG 0.623854 4.4:+70294451 None:intergenic
GAAATGGCTCACTGAGGACA+AGG 0.624424 4.4:-70294419 MS.gene93900:CDS
AGTAAAACAGCATCTGCAGG+AGG 0.624714 4.4:+70294070 None:intergenic
CATGGCACTCAAATCAGCCG+TGG 0.640463 4.4:-70294743 MS.gene93900:CDS
GCAGATGCAATCCACAACCA+TGG 0.642290 4.4:-70294709 MS.gene93900:CDS
ACAGGACACTAACTTTAGAA+GGG 0.644029 4.4:+70294641 None:intergenic
CTGATGAGAAGCATTGAAGG+AGG 0.666811 4.4:+70294520 None:intergenic
ACTCCAAACCTTCAAACACA+TGG 0.677292 4.4:+70294254 None:intergenic
GTAAAACAGCATCTGCAGGA+GGG 0.681270 4.4:+70294071 None:intergenic
CTCTAAGAAATGGCTCACTG+AGG 0.732533 4.4:-70294425 MS.gene93900:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTAAAGATATATTTTTTT+GGG - chr4.4:70294152-70294171 MS.gene93900:CDS 10.0%
!! TAAAAATATGAACTTATGAA+GGG + chr4.4:70294707-70294726 None:intergenic 15.0%
!!! AACGAAAATTTAAAATTTGT+TGG - chr4.4:70294058-70294077 MS.gene93900:intron 15.0%
!!! GAGTTAAAGATATATTTTTT+TGG - chr4.4:70294151-70294170 MS.gene93900:CDS 15.0%
!! CTAATTAATCTTTGCAATAA+AGG - chr4.4:70294479-70294498 MS.gene93900:CDS 20.0%
!! GTAAAAATATGAACTTATGA+AGG + chr4.4:70294708-70294727 None:intergenic 20.0%
!! TGTTCATTATCTTATATCAA+CGG + chr4.4:70294390-70294409 None:intergenic 20.0%
!!! AGAACTTGTTATAATAATGA+TGG - chr4.4:70294502-70294521 MS.gene93900:CDS 20.0%
!!! GAAAATTTAAAATTTGTTGG+TGG - chr4.4:70294061-70294080 MS.gene93900:intron 20.0%
! AAAAACATAATTGAGCTTAC+AGG + chr4.4:70294557-70294576 None:intergenic 25.0%
! GCATTACATTCTCTATTTAT+AGG + chr4.4:70294604-70294623 None:intergenic 25.0%
! TAATTAGTCATCAACTAAAC+TGG + chr4.4:70294466-70294485 None:intergenic 25.0%
!! GAGTAATTTTCGTATGTTAT+TGG - chr4.4:70294426-70294445 MS.gene93900:CDS 25.0%
!!! ACTTGTTATAATAATGATGG+TGG - chr4.4:70294505-70294524 MS.gene93900:CDS 25.0%
ACCGATCTTAAAATTGTCAA+CGG - chr4.4:70294323-70294342 MS.gene93900:CDS 30.0%
AGAGAATGTAATGCAAAGAA+AGG - chr4.4:70294610-70294629 MS.gene93900:CDS 30.0%
TGTAATGCAAAGAAAGGATA+CGG - chr4.4:70294616-70294635 MS.gene93900:CDS 30.0%
ACAGGAACGAAACATAATTG+AGG + chr4.4:70294539-70294558 None:intergenic 35.0%
ACCACAGGTTGTAAAAAACT+TGG - chr4.4:70294030-70294049 MS.gene93900:intron 35.0%
GAACGAAACATAATTGAGGT+GGG + chr4.4:70294535-70294554 None:intergenic 35.0%
GCAAAAACAACACTGATGAA+TGG - chr4.4:70293593-70293612 MS.gene93900:intron 35.0%
GCTAATTCTGTAAGAGTCAT+TGG + chr4.4:70293494-70293513 None:intergenic 35.0%
GGAAGAAAATGAAAAGCTAC+AGG + chr4.4:70294583-70294602 None:intergenic 35.0%
GGTCAAATACTATGCATTTG+AGG + chr4.4:70294009-70294028 None:intergenic 35.0%
TACAGGACACTAACTTTAGA+AGG + chr4.4:70293536-70293555 None:intergenic 35.0%
! AATACTATGCATTTGAGGTG+AGG + chr4.4:70294004-70294023 None:intergenic 35.0%
! ACAGGACACTAACTTTAGAA+GGG + chr4.4:70293535-70293554 None:intergenic 35.0%
! TGTTTGTCACCATTTGTTAC+CGG - chr4.4:70293689-70293708 MS.gene93900:intron 35.0%
!! TTCGATCATAAAAGCGTTAG+TGG + chr4.4:70294279-70294298 None:intergenic 35.0%
!!! AGTTTTTTACAACCTGTGGT+TGG + chr4.4:70294030-70294049 None:intergenic 35.0%
!!! GCCGTTGACAATTTTAAGAT+CGG + chr4.4:70294327-70294346 None:intergenic 35.0%
!!! TAAGAGTCATTGGTTTTCCA+TGG + chr4.4:70293484-70293503 None:intergenic 35.0%
ACACTGATGAATGGCAAAGA+AGG - chr4.4:70293602-70293621 MS.gene93900:intron 40.0%
ACTCCAAACCTTCAAACACA+TGG + chr4.4:70293922-70293941 None:intergenic 40.0%
AGTTAACTGCCGCTGATATA+AGG + chr4.4:70294226-70294245 None:intergenic 40.0%
CAGGTTGTAAAAAACTTGGC+TGG - chr4.4:70294034-70294053 MS.gene93900:intron 40.0%
CATAGTATTTGACCAACCAC+AGG - chr4.4:70294015-70294034 MS.gene93900:intron 40.0%
GAGTATGTTTCAAGATGCCA+TGG - chr4.4:70293853-70293872 MS.gene93900:intron 40.0%
GGAACGAAACATAATTGAGG+TGG + chr4.4:70294536-70294555 None:intergenic 40.0%
GTTAACTGCCGCTGATATAA+GGG + chr4.4:70294225-70294244 None:intergenic 40.0%
TAGCCATGTGTTTGAAGGTT+TGG - chr4.4:70293916-70293935 MS.gene93900:intron 40.0%
TATCGCTGAAAAATGTCGAG+CGG + chr4.4:70294250-70294269 None:intergenic 40.0%
TGAGGTGAGGAAAAGTTTCA+TGG + chr4.4:70293991-70294010 None:intergenic 40.0%
TTGAGTAAAACAGCATCTGC+AGG + chr4.4:70294109-70294128 None:intergenic 40.0%
! AGGGAACGAGCAATTTTACA+AGG + chr4.4:70294206-70294225 None:intergenic 40.0%
! GGGAACGAGCAATTTTACAA+GGG + chr4.4:70294205-70294224 None:intergenic 40.0%
!! AGCACGATTGTTTCTGCAAT+AGG - chr4.4:70294739-70294758 MS.gene93900:CDS 40.0%
!!! GCCAAGTTTTTTACAACCTG+TGG + chr4.4:70294034-70294053 None:intergenic 40.0%
!!! TCATTGGTTTTCCATGGTTG+TGG + chr4.4:70293478-70293497 None:intergenic 40.0%
!!! TGCAGATGCTGTTTTACTCA+AGG - chr4.4:70294108-70294127 MS.gene93900:CDS 40.0%
AGTAAAACAGCATCTGCAGG+AGG + chr4.4:70294106-70294125 None:intergenic 45.0%
ATCCATGTCGTCTCAACATG+TGG - chr4.4:70293720-70293739 MS.gene93900:intron 45.0%
ATCTGCTATGCCTAGTTCCA+CGG + chr4.4:70293450-70293469 None:intergenic 45.0%
CACAAGAGGCGAATCATTTC+GGG - chr4.4:70293796-70293815 MS.gene93900:intron 45.0%
CCTTCAATGCTTCTCATCAG+TGG - chr4.4:70293656-70293675 MS.gene93900:intron 45.0%
CTCAGTGAGCCATTTCTTAG+AGG + chr4.4:70293750-70293769 None:intergenic 45.0%
GAGTGTAGCCATGTGTTTGA+AGG - chr4.4:70293911-70293930 MS.gene93900:intron 45.0%
GATTTGAGAATCAGCTGCCA+TGG + chr4.4:70293873-70293892 None:intergenic 45.0%
GCACAAGAGGCGAATCATTT+CGG - chr4.4:70293795-70293814 MS.gene93900:intron 45.0%
GTAAAACAGCATCTGCAGGA+GGG + chr4.4:70294105-70294124 None:intergenic 45.0%
TGAAGCGATCCGGTAACAAA+TGG + chr4.4:70293701-70293720 None:intergenic 45.0%
TGCTCGTTCCCTTATATCAG+CGG - chr4.4:70294214-70294233 MS.gene93900:CDS 45.0%
! AGTCTCTTTGAGAGCACAAG+AGG - chr4.4:70293782-70293801 MS.gene93900:intron 45.0%
! CTCTAAGAAATGGCTCACTG+AGG - chr4.4:70293748-70293767 MS.gene93900:intron 45.0%
! GGACACTAACTTTAGAAGGG+TGG + chr4.4:70293532-70293551 None:intergenic 45.0%
! GTTTGAAGGTTTGGAGTCTC+TGG - chr4.4:70293925-70293944 MS.gene93900:intron 45.0%
! TGTGGCACTCCTCTAAGAAA+TGG - chr4.4:70293738-70293757 MS.gene93900:intron 45.0%
! TTGCGCTTGCTAACACACAA+TGG - chr4.4:70293563-70293582 MS.gene93900:intron 45.0%
! TTGGAGTCTCTGGTTGATGT+TGG - chr4.4:70293935-70293954 MS.gene93900:intron 45.0%
!! CCACTGATGAGAAGCATTGA+AGG + chr4.4:70293659-70293678 None:intergenic 45.0%
!! CTGATGAGAAGCATTGAAGG+AGG + chr4.4:70293656-70293675 None:intergenic 45.0%
AACGGTAGTTAACTGCCGCT+CGG + chr4.4:70294372-70294391 None:intergenic 50.0%
ACGACATGGATGAAGCGATC+CGG + chr4.4:70293711-70293730 None:intergenic 50.0%
CTCAAATCAGCCGTGGAACT+AGG - chr4.4:70293437-70293456 MS.gene93900:intron 50.0%
GAAATGGCTCACTGAGGACA+AGG - chr4.4:70293754-70293773 MS.gene93900:intron 50.0%
GCAGATGCAATCCACAACCA+TGG - chr4.4:70293464-70293483 MS.gene93900:intron 50.0%
TGCCACATGTTGAGACGACA+TGG + chr4.4:70293725-70293744 None:intergenic 50.0%
TGCCACTGAAAAATGCCGAG+CGG - chr4.4:70294354-70294373 MS.gene93900:CDS 50.0%
! GAGTCTCTGGTTGATGTTGG+AGG - chr4.4:70293938-70293957 MS.gene93900:intron 50.0%
! TCTCTGGTTGATGTTGGAGG+TGG - chr4.4:70293941-70293960 MS.gene93900:intron 50.0%
GCAAGCGCAAGAGACGATAC+AGG + chr4.4:70293553-70293572 None:intergenic 55.0%
GTTGATGTTGGAGGTGGCAC+AGG - chr4.4:70293947-70293966 MS.gene93900:intron 55.0%
!! TGCCGCTCGGCATTTTTCAG+TGG + chr4.4:70294359-70294378 None:intergenic 55.0%
GATGTTGGAGGTGGCACAGG+AGG - chr4.4:70293950-70293969 MS.gene93900:intron 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 70293431 70294764 70293431 ID=MS.gene93900
chr4.4 mRNA 70293431 70294764 70293431 ID=MS.gene93900.t1;Parent=MS.gene93900
chr4.4 exon 70294066 70294764 70294066 ID=MS.gene93900.t1.exon1;Parent=MS.gene93900.t1
chr4.4 CDS 70294066 70294764 70294066 ID=cds.MS.gene93900.t1;Parent=MS.gene93900.t1
chr4.4 exon 70293431 70293454 70293431 ID=MS.gene93900.t1.exon2;Parent=MS.gene93900.t1
chr4.4 CDS 70293431 70293454 70293431 ID=cds.MS.gene93900.t1;Parent=MS.gene93900.t1
Gene Sequence

>MS.gene93900

ATGGCACTCAAATCAGCCGTGGAACTAGGCATAGCAGATGCAATCCACAACCATGGAAAACCAATGACTCTTACAGAATTAGCTTCATCTTTGAAACTCCACCCTTCTAAAGTTAGTGTCCTGTATCGTCTCTTGCGCTTGCTAACACACAATGGTTTCTTTGCAAAAACAACACTGATGAATGGCAAAGAAGGAGAAGAAGAAACAACATATTCTCTCACTCCTCCTTCAATGCTTCTCATCAGTGGCAAATCAACATGTTTGTCACCATTTGTTACCGGATCGCTTCATCCATGTCGTCTCAACATGTGGCACTCCTCTAAGAAATGGCTCACTGAGGACAAGGAACTCAGTCTCTTTGAGAGCACAAGAGGCGAATCATTTCGGGACTATCTTAACAAAGATTCTGAATCTGATGAACTGAGTATGTTTCAAGATGCCATGGCAGCTGATTCTCAAATCTTTAACATTGCACTAAAAGAGTGTAGCCATGTGTTTGAAGGTTTGGAGTCTCTGGTTGATGTTGGAGGTGGCACAGGAGGTGTCACAAAACTTATCCATGAAACTTTTCCTCACCTCAAATGCATAGTATTTGACCAACCACAGGTTGTAAAAAACTTGGCTGGAAACGAAAATTTAAAATTTGTTGGTGGAGATATGTTCGAATCTATCCCTCCTGCAGATGCTGTTTTACTCAAGGTACGTTCACTAAATGTTATTGAGTTAAAGATATATTTTTTTGGGTAATTATGTTATGAAAATTAATCTTCTCCCTTGTAAAATTGCTCGTTCCCTTATATCAGCGGCAGTTAACTACCGCTCGACATTTTTCAGCGATAGAACTACCACTAACGCTTTTATGATCGAATGAACTGACTTAAAAAGTAATTGAACCGATCTTAAAATTGTCAACGGCAATTAGCTGCCACTGAAAAATGCCGAGCGGCAGTTAACTACCGTTGATATAAGATAATGAACAATTTCACAAGTGAGAAGAGTAATTTTCGTATGTTATTGGTGCGAAGTATTTTACCAGTTTAGTTGATGACTAATTAATCTTTGCAATAAAGGAGAACTTGTTATAATAATGATGGTGGATGACCCACCTCAATTATGTTTCGTTCCTGTAAGCTCAATTATGTTTTTGTTCCTGTAGCTTTTCATTTTCTTCCTATAAATAGAGAATGTAATGCAAAGAAAGGATACGGAATCAACACTTCTCACAATAATTACTTTTTTCTCTTTAGCAACTTTCTCTTCAATTCTCTCTTTTCCCTTCATAAGTTCATATTTTTACAACACGTTATCAGCACGATTGTTTCTGCAATAGGTAG
Protein sequence

>MS.gene93900.t1

MALKSAVELGIADAIHNHGKPMTLTELASSLKLHPSKVSVLYRLLRLLTHNGFFAKTTLMNGKEGEEETTYSLTPPSMLLISGKSTCLSPFVTGSLHPCRLNMWHSSKKWLTEDKELSLFESTRGESFRDYLNKDSESDELSMFQDAMAADSQIFNIALKECSHVFEGLESLVDVGGGTGGVTKLIHETFPHLKCIVFDQPQVVKNLAGNENLKFVGGDMFESIPPADAVLLKHDCFCNR