AlfalfaGEDB | MS.gene93954

M. sativa cultivar XinJiangDaYe / MS.gene93954

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93954.t1	XP_013455294.1	88.4	129	8	3	1	127	1	124	1.70E-52	215.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93954.t1	A0A072UTY2	88.4	129	8	3	1	127	1	124	1.2e-52	215.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050904	MS.gene93954	0.847235	1.31E-59	-1.69E-46
MS.gene055750	MS.gene93954	0.807714	4.42E-50	-1.69E-46
MS.gene05589	MS.gene93954	0.802895	4.52E-49	-1.69E-46
MS.gene056618	MS.gene93954	-0.804764	1.85E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93954.t1	MTR_4g033475	90.698	129	5	3	1	127	1	124	2.03e-72	216

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93954.t1	AT2G44870	61.333	75	29	0	53	127	46	120	1.01e-16	73.9

Find 28 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTGGCGGTGACGGCGGTTGA+AGG	0.301958	4.3:+66209529	None:intergenic
CAACAATAAACCAAATGATA+TGG	0.333595	4.3:-66209471	MS.gene93954:CDS
AACTAACCCTAGGCCAGTTA+AGG	0.381232	4.3:+66209237	None:intergenic
AAAACCCTATCCATATCATT+TGG	0.381924	4.3:+66209461	None:intergenic
TTCTTGAATGAGACTGTTAC+GGG	0.394388	4.3:+66209497	None:intergenic
AGGCCAGTTAAGGGCAAAAT+CGG	0.432734	4.3:+66209247	None:intergenic
ATGAGACTGTTACGGGTTGC+CGG	0.446119	4.3:+66209504	None:intergenic
GTTGCCGGTGGTGGCGGTGA+CGG	0.453861	4.3:+66209519	None:intergenic
CGGTGACGGCGGTTGAAGGG+TGG	0.455893	4.3:+66209533	None:intergenic
GTTCTTGAATGAGACTGTTA+CGG	0.457562	4.3:+66209496	None:intergenic
CTGTTACGGGTTGCCGGTGG+TGG	0.471361	4.3:+66209510	None:intergenic
ATAAACCAAATGATATGGAT+AGG	0.489253	4.3:-66209466	MS.gene93954:CDS
GGTGACGGCGGTTGAAGGGT+GGG	0.492527	4.3:+66209534	None:intergenic
TGGCGGTGACGGCGGTTGAA+GGG	0.495800	4.3:+66209530	None:intergenic
ACTAACCCTAGGCCAGTTAA+GGG	0.512662	4.3:+66209238	None:intergenic
CGGTTGAAGGGTGGGAGGTG+TGG	0.516128	4.3:+66209542	None:intergenic
TTACGGGTTGCCGGTGGTGG+CGG	0.518901	4.3:+66209513	None:intergenic
ACCGCCGTCACCGCCACCAC+CGG	0.543092	4.3:-66209523	MS.gene93954:CDS
TAAACCAAATGATATGGATA+GGG	0.546958	4.3:-66209465	MS.gene93954:CDS
AAGCTAAGAAAACTGCTGAA+AGG	0.566137	4.3:-66209388	MS.gene93954:CDS
ACAATGACAAACACATGCAC+AGG	0.572025	4.3:-66209596	MS.gene93954:CDS
GCCGGTGGTGGCGGTGACGG+CGG	0.572648	4.3:+66209522	None:intergenic
GACGGCGGTTGAAGGGTGGG+AGG	0.588812	4.3:+66209537	None:intergenic
GGAAGTAACTAACTAACCCT+AGG	0.590516	4.3:+66209227	None:intergenic
GCGTGGCAAAACGCTAACGA+TGG	0.681729	4.3:-66209431	MS.gene93954:CDS
GTTTAGACACGGAATACTGA+CGG	0.693461	4.3:+66209360	None:intergenic
AGACTGTTACGGGTTGCCGG+TGG	0.709074	4.3:+66209507	None:intergenic
AGAAGCAAGACGTTTAGACA+CGG	0.720219	4.3:+66209349	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATAAACCAAATGATATGGAT+AGG	-	chr4.3:66209371-66209390	MS.gene93954:CDS	25.0%
!	CAACAATAAACCAAATGATA+TGG	-	chr4.3:66209366-66209385	MS.gene93954:CDS	25.0%
!	TAAACCAAATGATATGGATA+GGG	-	chr4.3:66209372-66209391	MS.gene93954:CDS	25.0%
!!!	ATTGATCGTGATTTTGAAAT+TGG	-	chr4.3:66209544-66209563	MS.gene93954:CDS	25.0%
	AAAACCCTATCCATATCATT+TGG	+	chr4.3:66209379-66209398	None:intergenic	30.0%
!	GTTGTTGTTGTTGTTGATAT+CGG	+	chr4.3:66209276-66209295	None:intergenic	30.0%
	AAGCTAAGAAAACTGCTGAA+AGG	-	chr4.3:66209449-66209468	MS.gene93954:CDS	35.0%
	GTTCTTGAATGAGACTGTTA+CGG	+	chr4.3:66209344-66209363	None:intergenic	35.0%
	TTCTTGAATGAGACTGTTAC+GGG	+	chr4.3:66209343-66209362	None:intergenic	35.0%
	ACAATGACAAACACATGCAC+AGG	-	chr4.3:66209241-66209260	MS.gene93954:CDS	40.0%
	AGAAGCAAGACGTTTAGACA+CGG	+	chr4.3:66209491-66209510	None:intergenic	40.0%
	GTTTAGACACGGAATACTGA+CGG	+	chr4.3:66209480-66209499	None:intergenic	40.0%
!!!	ATAGGGTTTTGAAAGAAGCG+TGG	-	chr4.3:66209389-66209408	MS.gene93954:CDS	40.0%
	AACTAACCCTAGGCCAGTTA+AGG	+	chr4.3:66209603-66209622	None:intergenic	45.0%
	ACTAACCCTAGGCCAGTTAA+GGG	+	chr4.3:66209602-66209621	None:intergenic	45.0%
	AGGCCAGTTAAGGGCAAAAT+CGG	+	chr4.3:66209593-66209612	None:intergenic	45.0%
!	CTTCCGATTTTGCCCTTAAC+TGG	-	chr4.3:66209587-66209606	MS.gene93954:CDS	45.0%
!!	ATTTTGCCCTTAACTGGCCT+AGG	-	chr4.3:66209593-66209612	MS.gene93954:CDS	45.0%
!!	TTTTGCCCTTAACTGGCCTA+GGG	-	chr4.3:66209594-66209613	MS.gene93954:CDS	45.0%
!!!	ACGCTAACGATGGTTTTGAG+CGG	-	chr4.3:66209416-66209435	MS.gene93954:CDS	45.0%
!	ATGAGACTGTTACGGGTTGC+CGG	+	chr4.3:66209336-66209355	None:intergenic	50.0%
	GCGTGGCAAAACGCTAACGA+TGG	-	chr4.3:66209406-66209425	MS.gene93954:CDS	55.0%
!	AGACTGTTACGGGTTGCCGG+TGG	+	chr4.3:66209333-66209352	None:intergenic	60.0%
	CGGTTGAAGGGTGGGAGGTG+TGG	+	chr4.3:66209298-66209317	None:intergenic	65.0%
!	CTGTTACGGGTTGCCGGTGG+TGG	+	chr4.3:66209330-66209349	None:intergenic	65.0%
!	GGTGACGGCGGTTGAAGGGT+GGG	+	chr4.3:66209306-66209325	None:intergenic	65.0%
!	TGGCGGTGACGGCGGTTGAA+GGG	+	chr4.3:66209310-66209329	None:intergenic	65.0%
!	TTACGGGTTGCCGGTGGTGG+CGG	+	chr4.3:66209327-66209346	None:intergenic	65.0%
	GACGGCGGTTGAAGGGTGGG+AGG	+	chr4.3:66209303-66209322	None:intergenic	70.0%
!	CGGTGACGGCGGTTGAAGGG+TGG	+	chr4.3:66209307-66209326	None:intergenic	70.0%
!	GTGGCGGTGACGGCGGTTGA+AGG	+	chr4.3:66209311-66209330	None:intergenic	70.0%
!!	GTTGCCGGTGGTGGCGGTGA+CGG	+	chr4.3:66209321-66209340	None:intergenic	70.0%
	ACCGCCGTCACCGCCACCAC+CGG	-	chr4.3:66209314-66209333	MS.gene93954:CDS	75.0%
!!!	GCCGGTGGTGGCGGTGACGG+CGG	+	chr4.3:66209318-66209337	None:intergenic	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	66209235	66209624	66209235	ID=MS.gene93954
chr4.3	mRNA	66209235	66209624	66209235	ID=MS.gene93954.t1;Parent=MS.gene93954
chr4.3	exon	66209235	66209624	66209235	ID=MS.gene93954.t1.exon1;Parent=MS.gene93954.t1
chr4.3	CDS	66209235	66209624	66209235	ID=cds.MS.gene93954.t1;Parent=MS.gene93954.t1

Gene Sequence

>MS.gene93954

ATGTTCACAATGACAAACACATGCACAGGAACATCACTCCGATATCAACAACAACAACAACCACACCTCCCACCCTTCAACCGCCGTCACCGCCACCACCGGCAACCCGTAACAGTCTCATTCAAGAACAACAACAATAAACCAAATGATATGGATAGGGTTTTGAAAGAAGCGTGGCAAAACGCTAACGATGGTTTTGAGCGGTTATTATTTGAAGCTAAGAAAACTGCTGAAAGGATTGACCGTCAGTATTCCGTGTCTAAACGTCTTGCTTCTGTTGCTTCTGCTGCTGCTGATAGAGCTCGTGAAATTGATCGTGATTTTGAAATTGGTGTTAAATATCGTAACTTTTCTTCCGATTTTGCCCTTAACTGGCCTAGGGTTAGTTAG

Protein sequence

>MS.gene93954.t1

MFTMTNTCTGTSLRYQQQQQPHLPPFNRRHRHHRQPVTVSFKNNNNKPNDMDRVLKEAWQNANDGFERLLFEAKKTAERIDRQYSVSKRLASVASAAADRAREIDRDFEIGVKYRNFSSDFALNWPRVS