Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94248.t1 | XP_003623155.2 | 100 | 99 | 0 | 0 | 202 | 300 | 1 | 99 | 9.80E-48 | 200.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94248.t1 | Q9SI54 | 94.7 | 94 | 5 | 0 | 202 | 295 | 1 | 94 | 1.9e-44 | 181.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94248.t1 | G7L5K6 | 100.0 | 99 | 0 | 0 | 202 | 300 | 1 | 99 | 7.1e-48 | 200.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051241 | MS.gene94248 | 0.837157 | 6.13E-57 | -1.69E-46 |
| MS.gene05134 | MS.gene94248 | 0.80181 | 7.55E-49 | -1.69E-46 |
| MS.gene057705 | MS.gene94248 | 0.825763 | 3.93E-54 | -1.69E-46 |
| MS.gene060429 | MS.gene94248 | 0.808937 | 2.42E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94248.t1 | MTR_7g065100 | 100.000 | 99 | 0 | 0 | 202 | 300 | 1 | 99 | 2.37e-64 | 197 |
| MS.gene94248.t1 | MTR_7g065100 | 79.661 | 59 | 11 | 1 | 14 | 72 | 14 | 71 | 1.74e-22 | 90.1 |
| MS.gene94248.t1 | MTR_8g036205 | 96.512 | 86 | 3 | 0 | 202 | 287 | 1 | 86 | 5.21e-50 | 162 |
| MS.gene94248.t1 | MTR_8g036205 | 77.586 | 58 | 12 | 1 | 14 | 71 | 14 | 70 | 3.63e-21 | 87.4 |
| MS.gene94248.t1 | MTR_1g041340 | 73.469 | 49 | 13 | 0 | 101 | 149 | 269 | 317 | 5.19e-18 | 83.2 |
| MS.gene94248.t1 | MTR_1g046400 | 41.176 | 85 | 49 | 1 | 110 | 194 | 39 | 122 | 2.71e-14 | 68.6 |
| MS.gene94248.t1 | MTR_5g014470 | 49.153 | 59 | 30 | 0 | 141 | 199 | 28 | 86 | 9.98e-13 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94248.t1 | AT2G03870 | 90.816 | 98 | 9 | 0 | 202 | 299 | 1 | 98 | 3.76e-57 | 179 |
| MS.gene94248.t1 | AT2G03870 | 74.576 | 59 | 14 | 1 | 14 | 72 | 14 | 71 | 2.25e-20 | 84.3 |
| MS.gene94248.t1 | AT2G03870 | 90.816 | 98 | 9 | 0 | 202 | 299 | 1 | 98 | 3.76e-57 | 179 |
| MS.gene94248.t1 | AT2G03870 | 74.576 | 59 | 14 | 1 | 14 | 72 | 14 | 71 | 2.25e-20 | 84.3 |
| MS.gene94248.t1 | AT2G23930 | 42.254 | 71 | 32 | 2 | 211 | 281 | 9 | 70 | 1.82e-11 | 59.7 |
| MS.gene94248.t1 | AT3G11500 | 39.437 | 71 | 34 | 1 | 211 | 281 | 9 | 70 | 7.63e-11 | 57.8 |
| MS.gene94248.t1 | AT3G11500 | 39.437 | 71 | 34 | 1 | 211 | 281 | 9 | 70 | 7.63e-11 | 57.8 |
Find 67 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGAAAATCCTGGAATATC+TGG | 0.224493 | 7.3:+40853638 | MS.gene94248:CDS |
| CGTGATGATATTCCCGTATT+TGG | 0.229723 | 7.3:+40849331 | MS.gene94248:CDS |
| GCATTGTTAGCTCTGTTTCA+TGG | 0.278391 | 7.3:+40853746 | MS.gene94248:CDS |
| AATGATGGCAGTTGGCTATA+TGG | 0.314232 | 7.3:+40853683 | MS.gene94248:CDS |
| CCTCCTCGTCGCTCAATGAT+AGG | 0.330178 | 7.3:+40852277 | MS.gene94248:CDS |
| TTGAAAGCAGCTTGGGATAA+AGG | 0.335049 | 7.3:+40853770 | MS.gene94248:CDS |
| AGGGAGGAAAGAAACAGTTT+TGG | 0.368734 | 7.3:+40856130 | MS.gene94248:CDS |
| AATCCTGGAATATCTGGTTT+TGG | 0.403508 | 7.3:+40853644 | MS.gene94248:CDS |
| ATGATGGCAGTTGGCTATAT+GGG | 0.403955 | 7.3:+40853684 | MS.gene94248:CDS |
| CCCGGACAGGTTTATCAGTT+AGG | 0.428471 | 7.3:+40852181 | MS.gene94248:intron |
| TCATGGCTTGAAAGCAGCTT+GGG | 0.429941 | 7.3:+40853763 | MS.gene94248:CDS |
| GCCCCATCTGGTTGAAGAAA+GGG | 0.432718 | 7.3:-40860754 | None:intergenic |
| CTTGTGTCAACAGACAGATT+TGG | 0.433206 | 7.3:+40852225 | MS.gene94248:CDS |
| ATTGTTGCCATCAACATTCA+AGG | 0.433581 | 7.3:-40853615 | None:intergenic |
| GCCGTTGCCTTGAATGTTGA+TGG | 0.433924 | 7.3:+40853608 | MS.gene94248:CDS |
| TGTTGACACAAGTGCAAGTC+TGG | 0.447628 | 7.3:-40852214 | None:intergenic |
| TCAATTACTTAACCTTGTAC+TGG | 0.449744 | 7.3:+40850506 | MS.gene94248:CDS |
| CGCCCCATCTGGTTGAAGAA+AGG | 0.450498 | 7.3:-40860755 | None:intergenic |
| TCTCATCGGTACCATCAGTT+GGG | 0.463107 | 7.3:-40860723 | None:intergenic |
| AGTTGGCTATATGGGTTTGC+TGG | 0.468213 | 7.3:+40853692 | MS.gene94248:CDS |
| ATTCACTCTTACGCCCCATC+TGG | 0.472924 | 7.3:-40860766 | None:intergenic |
| TCAATTACTTAATCTTGTAC+TGG | 0.475275 | 7.3:+40858856 | MS.gene94248:CDS |
| TTCATGGCTTGAAAGCAGCT+TGG | 0.490614 | 7.3:+40853762 | MS.gene94248:CDS |
| CAAGTGCAAGTCTGGTGGGC+AGG | 0.491076 | 7.3:-40852206 | None:intergenic |
| ACCCCTTTCTTCAACCAGAT+GGG | 0.493242 | 7.3:+40860752 | MS.gene94248:CDS |
| GATGATATTCCCGTATTTGG+CGG | 0.499269 | 7.3:+40849334 | MS.gene94248:CDS |
| CCACCAAAACCAGATATTCC+AGG | 0.504894 | 7.3:-40853647 | None:intergenic |
| AACCCCTTTCTTCAACCAGA+TGG | 0.507354 | 7.3:+40860751 | MS.gene94248:CDS |
| TTGGCATCCGGAGGAGAGTA+CGG | 0.510694 | 7.3:+40853586 | MS.gene94248:CDS |
| TCAGTGACAGGAACTTTGAA+AGG | 0.518990 | 7.3:+40850477 | MS.gene94248:intron |
| TCAGTGACAGGAACTTTGAA+AGG | 0.518990 | 7.3:+40858827 | MS.gene94248:intron |
| ATCTCATCGGTACCATCAGT+TGG | 0.519831 | 7.3:-40860724 | None:intergenic |
| ACTGATCAGACAAGAAGTCT+TGG | 0.531760 | 7.3:+40851742 | MS.gene94248:CDS |
| CAACAGCTTCGTCCAGTACA+AGG | 0.533258 | 7.3:-40850518 | None:intergenic |
| TATGGGTTTGCTGGAAATGT+TGG | 0.533761 | 7.3:+40853701 | MS.gene94248:CDS |
| GCAACGGCCGTACTCTCCTC+CGG | 0.534788 | 7.3:-40853593 | None:intergenic |
| GCCAAGACTTCTTGTCTGAT+CGG | 0.535649 | 7.3:-40859892 | None:intergenic |
| TCGTTACGTGACTTGGCATC+CGG | 0.537126 | 7.3:+40853574 | MS.gene94248:CDS |
| TGCGTAGGAATGATGGCAGT+TGG | 0.540845 | 7.3:+40853675 | MS.gene94248:CDS |
| GCTAAAATGCAGGTTTGTAG+AGG | 0.541478 | 7.3:+40860679 | MS.gene94248:intron |
| ACCGATCAGACAAGAAGTCT+TGG | 0.542240 | 7.3:+40859891 | MS.gene94248:CDS |
| TAGGTGGTCGTTACGTGACT+TGG | 0.546523 | 7.3:+40853567 | MS.gene94248:intron |
| AAGACTTCTTGTCTGATCAG+TGG | 0.561348 | 7.3:-40851740 | None:intergenic |
| GGCGTTCTGCGTAGGAATGA+TGG | 0.563070 | 7.3:+40853668 | MS.gene94248:CDS |
| CAATGCTACATGTATGATTG+TGG | 0.572266 | 7.3:-40853729 | None:intergenic |
| TTGGCAAAATTCGTTGACAA+AGG | 0.572355 | 7.3:+40856155 | MS.gene94248:CDS |
| CCCCATCTGGTTGAAGAAAG+GGG | 0.574538 | 7.3:-40860753 | None:intergenic |
| CCCCTTTCTTCAACCAGATG+GGG | 0.577044 | 7.3:+40860753 | MS.gene94248:CDS |
| GCCATCAACATTCAAGGCAA+CGG | 0.590154 | 7.3:-40853609 | None:intergenic |
| ATGCTTGTGTCCCCAACTGA+TGG | 0.595021 | 7.3:+40860712 | MS.gene94248:CDS |
| TTGCTACTCTGACTCGACGC+TGG | 0.603793 | 7.3:+40853808 | MS.gene94248:CDS |
| AGGATTACCTTGTCTACCAC+CGG | 0.613364 | 7.3:-40856195 | None:intergenic |
| TATTCAGTTAGTTTCAACCA+TGG | 0.615077 | 7.3:+40853832 | MS.gene94248:CDS |
| GGTGTTCAAGTTAAGCTCAC+CGG | 0.616462 | 7.3:+40856176 | MS.gene94248:CDS |
| AAGCTCACCGGTGGTAGACA+AGG | 0.616471 | 7.3:+40856188 | MS.gene94248:CDS |
| GTATTTGGCGGAAAACAAGT+TGG | 0.628988 | 7.3:+40849346 | MS.gene94248:CDS |
| AAAGGGGTTTGCAATCTCAT+CGG | 0.630182 | 7.3:-40860737 | None:intergenic |
| CTCATCGGTACCATCAGTTG+GGG | 0.631525 | 7.3:-40860722 | None:intergenic |
| GACACAAGTGCAAGTCTGGT+GGG | 0.637367 | 7.3:-40852210 | None:intergenic |
| CCTATCATTGAGCGACGAGG+AGG | 0.639034 | 7.3:-40852277 | None:intergenic |
| TGACACAAGTGCAAGTCTGG+TGG | 0.658374 | 7.3:-40852211 | None:intergenic |
| TTACGTGACTTGGCATCCGG+AGG | 0.686026 | 7.3:+40853577 | MS.gene94248:CDS |
| GTTCAAGTTAAGCTCACCGG+TGG | 0.687790 | 7.3:+40856179 | MS.gene94248:CDS |
| GAAGCTGTTGAGTTTCTGAG+AGG | 0.702935 | 7.3:+40850531 | MS.gene94248:CDS |
| GAAGCTGTTGAGTTTCTGAG+AGG | 0.702935 | 7.3:+40858881 | MS.gene94248:CDS |
| TATGTGATTAACAGTCAGGG+AGG | 0.727021 | 7.3:+40856114 | MS.gene94248:intron |
| GAACCTATCATTGAGCGACG+AGG | 0.742728 | 7.3:-40852280 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 40849280 | 40860780 | 40849280 | ID=MS.gene94248 |
| chr7.3 | mRNA | 40849280 | 40860780 | 40849280 | ID=MS.gene94248.t1;Parent=MS.gene94248 |
| chr7.3 | exon | 40849280 | 40849367 | 40849280 | ID=MS.gene94248.t1.exon1;Parent=MS.gene94248.t1 |
| chr7.3 | CDS | 40849280 | 40849367 | 40849280 | ID=cds.MS.gene94248.t1;Parent=MS.gene94248.t1 |
| chr7.3 | exon | 40850481 | 40850552 | 40850481 | ID=MS.gene94248.t1.exon2;Parent=MS.gene94248.t1 |
| chr7.3 | CDS | 40850481 | 40850552 | 40850481 | ID=cds.MS.gene94248.t1;Parent=MS.gene94248.t1 |
| chr7.3 | exon | 40851719 | 40851771 | 40851719 | ID=MS.gene94248.t1.exon3;Parent=MS.gene94248.t1 |
| chr7.3 | CDS | 40851719 | 40851771 | 40851719 | ID=cds.MS.gene94248.t1;Parent=MS.gene94248.t1 |
| chr7.3 | exon | 40852190 | 40852298 | 40852190 | ID=MS.gene94248.t1.exon4;Parent=MS.gene94248.t1 |
| chr7.3 | CDS | 40852190 | 40852298 | 40852190 | ID=cds.MS.gene94248.t1;Parent=MS.gene94248.t1 |
| chr7.3 | exon | 40853570 | 40853853 | 40853570 | ID=MS.gene94248.t1.exon5;Parent=MS.gene94248.t1 |
| chr7.3 | CDS | 40853570 | 40853853 | 40853570 | ID=cds.MS.gene94248.t1;Parent=MS.gene94248.t1 |
| chr7.3 | exon | 40856128 | 40856209 | 40856128 | ID=MS.gene94248.t1.exon6;Parent=MS.gene94248.t1 |
| chr7.3 | CDS | 40856128 | 40856209 | 40856128 | ID=cds.MS.gene94248.t1;Parent=MS.gene94248.t1 |
| chr7.3 | exon | 40858831 | 40858902 | 40858831 | ID=MS.gene94248.t1.exon7;Parent=MS.gene94248.t1 |
| chr7.3 | CDS | 40858831 | 40858902 | 40858831 | ID=cds.MS.gene94248.t1;Parent=MS.gene94248.t1 |
| chr7.3 | exon | 40859868 | 40859920 | 40859868 | ID=MS.gene94248.t1.exon8;Parent=MS.gene94248.t1 |
| chr7.3 | CDS | 40859868 | 40859920 | 40859868 | ID=cds.MS.gene94248.t1;Parent=MS.gene94248.t1 |
| chr7.3 | exon | 40860691 | 40860780 | 40860691 | ID=MS.gene94248.t1.exon9;Parent=MS.gene94248.t1 |
| chr7.3 | CDS | 40860691 | 40860780 | 40860691 | ID=cds.MS.gene94248.t1;Parent=MS.gene94248.t1 |
| Gene Sequence |
| Protein sequence |
