Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94566.t1 | XP_003596709.2 | 91.4 | 233 | 20 | 0 | 1 | 233 | 1 | 233 | 2.60E-112 | 414.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94566.t1 | G7IM74 | 91.4 | 233 | 20 | 0 | 1 | 233 | 1 | 233 | 1.8e-112 | 414.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056677 | MS.gene94566 | 0.801871 | 7.34E-49 | -1.69E-46 |
| MS.gene05952 | MS.gene94566 | 0.806922 | 6.50E-50 | -1.69E-46 |
| MS.gene060012 | MS.gene94566 | 0.802963 | 4.37E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94566.t1 | MTR_2g083910 | 96.996 | 233 | 7 | 0 | 1 | 233 | 1 | 233 | 5.07e-165 | 455 |
| MS.gene94566.t1 | MTR_2g083910 | 96.970 | 231 | 7 | 0 | 1 | 231 | 1 | 231 | 7.26e-163 | 451 |
| MS.gene94566.t1 | MTR_4g080340 | 50.000 | 260 | 82 | 6 | 1 | 233 | 1 | 239 | 4.10e-63 | 197 |
| MS.gene94566.t1 | MTR_8g086820 | 45.382 | 249 | 113 | 7 | 1 | 233 | 1 | 242 | 1.14e-55 | 178 |
| MS.gene94566.t1 | MTR_2g016760 | 44.167 | 240 | 116 | 5 | 1 | 232 | 1 | 230 | 1.27e-53 | 177 |
| MS.gene94566.t1 | MTR_2g078620 | 47.059 | 102 | 42 | 5 | 134 | 230 | 225 | 319 | 1.70e-18 | 82.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94566.t1 | AT2G42760 | 37.363 | 273 | 124 | 9 | 1 | 233 | 1 | 266 | 2.96e-36 | 129 |
Find 38 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTATCAGAGCTTGAGTTTA+AGG | 0.133074 | 2.4:-15623687 | MS.gene94566:CDS |
| TAAGGAGCTAAAAGGGTTTA+TGG | 0.192552 | 2.4:-15623669 | MS.gene94566:CDS |
| CTTGAGTTTAAGGAGCTAAA+AGG | 0.195416 | 2.4:-15623677 | MS.gene94566:CDS |
| TTGTTAATCCATTGCTTAAT+TGG | 0.205608 | 2.4:-15623496 | MS.gene94566:CDS |
| GCTAAAAGGGTTTATGGATT+TGG | 0.214083 | 2.4:-15623663 | MS.gene94566:CDS |
| TGAAAGATAATCTTAAGTTT+TGG | 0.252639 | 2.4:-15623442 | MS.gene94566:CDS |
| CTAAAAGGGTTTATGGATTT+GGG | 0.263401 | 2.4:-15623662 | MS.gene94566:CDS |
| GCCTTATTTATCTGAGGCTT+GGG | 0.273923 | 2.4:-15623540 | MS.gene94566:CDS |
| TCTGAGGAAGATAAAGATTC+AGG | 0.292013 | 2.4:-15623629 | MS.gene94566:CDS |
| ACTTCTTTGCCTGAGAGAAT+TGG | 0.350310 | 2.4:+15623809 | None:intergenic |
| GGCCTTATTTATCTGAGGCT+TGG | 0.364492 | 2.4:-15623541 | MS.gene94566:CDS |
| GAATTCCAACACTTCAAGTT+AGG | 0.372690 | 2.4:-15623922 | MS.gene94566:CDS |
| CCCCAAGCCTCAGATAAATA+AGG | 0.382221 | 2.4:+15623539 | None:intergenic |
| TGAAACTGTTTGATATCAAC+TGG | 0.384594 | 2.4:-15624072 | MS.gene94566:CDS |
| GAAAACTTAGGCACGAAGAT+TGG | 0.434650 | 2.4:-15623887 | MS.gene94566:CDS |
| GGATTGGTTTCTTTGATACC+AGG | 0.467028 | 2.4:-15623608 | MS.gene94566:CDS |
| TGTGATGTGAGGACAGAGTT+TGG | 0.472758 | 2.4:+15623842 | None:intergenic |
| TTGAGTTTAAGGAGCTAAAA+GGG | 0.477612 | 2.4:-15623676 | MS.gene94566:CDS |
| AATTGGAGGGTACCAACTCT+TGG | 0.482818 | 2.4:-15623479 | MS.gene94566:CDS |
| AAGCTGAAGCCAATTCTCTC+AGG | 0.492170 | 2.4:-15623818 | MS.gene94566:CDS |
| TTCTTCCACTTGAGACATAG+AGG | 0.502500 | 2.4:+15623973 | None:intergenic |
| CACTTTCATCAACATTGTGT+TGG | 0.505818 | 2.4:+15623573 | None:intergenic |
| AAAGACCTAACTTGAAGTGT+TGG | 0.513872 | 2.4:+15623917 | None:intergenic |
| TTAATCCATTGCTTAATTGG+AGG | 0.514757 | 2.4:-15623493 | MS.gene94566:CDS |
| TTGATGAAAGTGTGATTAGT+AGG | 0.517023 | 2.4:-15623562 | MS.gene94566:CDS |
| GGAAGATAAAGATTCAGGAT+TGG | 0.523132 | 2.4:-15623624 | MS.gene94566:CDS |
| TAATCCATTGCTTAATTGGA+GGG | 0.524602 | 2.4:-15623492 | MS.gene94566:CDS |
| CATAGAACGAGACTTAGAAA+AGG | 0.547413 | 2.4:-15623728 | MS.gene94566:CDS |
| TAGTAGGCCTTATTTATCTG+AGG | 0.548733 | 2.4:-15623546 | MS.gene94566:CDS |
| CCTTATTTATCTGAGGCTTG+GGG | 0.550864 | 2.4:-15623539 | MS.gene94566:CDS |
| TCAATCTCATTGCCAAGAGT+TGG | 0.554919 | 2.4:+15623467 | None:intergenic |
| TTCAACCTCTATGTCTCAAG+TGG | 0.558037 | 2.4:-15623978 | MS.gene94566:CDS |
| AGTAATGTTGTTGAATGTAA+AGG | 0.562510 | 2.4:+15624007 | None:intergenic |
| GATTGGTTTCTTTGATACCA+GGG | 0.577636 | 2.4:-15623607 | MS.gene94566:CDS |
| TGTTGATTGTTATCTTACAA+TGG | 0.583010 | 2.4:+15623403 | None:intergenic |
| AATTCTCTCAGGCAAAGAAG+TGG | 0.596666 | 2.4:-15623807 | MS.gene94566:CDS |
| GGTACCCTCCAATTAAGCAA+TGG | 0.611167 | 2.4:+15623488 | None:intergenic |
| GCTTCAGCTTCTGTGATGTG+AGG | 0.642042 | 2.4:+15623831 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAAAGATAATCTTAAGTTTT+GGG | - | chr2.4:15624062-15624081 | MS.gene94566:CDS | 20.0% |
| !!! | TGAAAGATAATCTTAAGTTT+TGG | - | chr2.4:15624061-15624080 | MS.gene94566:CDS | 20.0% |
| ! | AGTAATGTTGTTGAATGTAA+AGG | + | chr2.4:15623499-15623518 | None:intergenic | 25.0% |
| ! | TTGTTAATCCATTGCTTAAT+TGG | - | chr2.4:15624007-15624026 | MS.gene94566:CDS | 25.0% |
| !!! | TGAAGATGTTTTTTTGTTGT+TGG | + | chr2.4:15623476-15623495 | None:intergenic | 25.0% |
| AGATGTAACCGATACAAAAA+AGG | - | chr2.4:15623738-15623757 | MS.gene94566:CDS | 30.0% | |
| CTAAAAGGGTTTATGGATTT+GGG | - | chr2.4:15623841-15623860 | MS.gene94566:CDS | 30.0% | |
| TAATCCATTGCTTAATTGGA+GGG | - | chr2.4:15624011-15624030 | MS.gene94566:CDS | 30.0% | |
| TGAAACTGTTTGATATCAAC+TGG | - | chr2.4:15623431-15623450 | MS.gene94566:CDS | 30.0% | |
| TTAATCCATTGCTTAATTGG+AGG | - | chr2.4:15624010-15624029 | MS.gene94566:CDS | 30.0% | |
| TTGAGTTTAAGGAGCTAAAA+GGG | - | chr2.4:15623827-15623846 | MS.gene94566:CDS | 30.0% | |
| TTTATCAGAGCTTGAGTTTA+AGG | - | chr2.4:15623816-15623835 | MS.gene94566:CDS | 30.0% | |
| ! | TTGATGAAAGTGTGATTAGT+AGG | - | chr2.4:15623941-15623960 | MS.gene94566:CDS | 30.0% |
| AAAGACCTAACTTGAAGTGT+TGG | + | chr2.4:15623589-15623608 | None:intergenic | 35.0% | |
| CACTTTCATCAACATTGTGT+TGG | + | chr2.4:15623933-15623952 | None:intergenic | 35.0% | |
| CATAGAACGAGACTTAGAAA+AGG | - | chr2.4:15623775-15623794 | MS.gene94566:CDS | 35.0% | |
| CTTGAGTTTAAGGAGCTAAA+AGG | - | chr2.4:15623826-15623845 | MS.gene94566:CDS | 35.0% | |
| GAAAAGGAAGAAAAACTGCT+AGG | - | chr2.4:15623791-15623810 | MS.gene94566:CDS | 35.0% | |
| GCTAAAAGGGTTTATGGATT+TGG | - | chr2.4:15623840-15623859 | MS.gene94566:CDS | 35.0% | |
| GGAAGATAAAGATTCAGGAT+TGG | - | chr2.4:15623879-15623898 | MS.gene94566:CDS | 35.0% | |
| TAAGGAGCTAAAAGGGTTTA+TGG | - | chr2.4:15623834-15623853 | MS.gene94566:CDS | 35.0% | |
| TCTGAGGAAGATAAAGATTC+AGG | - | chr2.4:15623874-15623893 | MS.gene94566:CDS | 35.0% | |
| ! | GAATTCCAACACTTCAAGTT+AGG | - | chr2.4:15623581-15623600 | MS.gene94566:CDS | 35.0% |
| ! | TCTTTTAGCGACGAAAACTT+AGG | - | chr2.4:15623604-15623623 | MS.gene94566:CDS | 35.0% |
| !! | GATTGGTTTCTTTGATACCA+GGG | - | chr2.4:15623896-15623915 | MS.gene94566:CDS | 35.0% |
| !! | TAGTAGGCCTTATTTATCTG+AGG | - | chr2.4:15623957-15623976 | MS.gene94566:CDS | 35.0% |
| !! | TTTGGGATTTGTGTTTTCTG+AGG | - | chr2.4:15623858-15623877 | MS.gene94566:CDS | 35.0% |
| !!! | ATGACTCGCCTTTTTTGTAT+CGG | + | chr2.4:15623749-15623768 | None:intergenic | 35.0% |
| AATTCTCTCAGGCAAAGAAG+TGG | - | chr2.4:15623696-15623715 | MS.gene94566:CDS | 40.0% | |
| ACTTCTTTGCCTGAGAGAAT+TGG | + | chr2.4:15623697-15623716 | None:intergenic | 40.0% | |
| GAAAACTTAGGCACGAAGAT+TGG | - | chr2.4:15623616-15623635 | MS.gene94566:CDS | 40.0% | |
| TCAATCTCATTGCCAAGAGT+TGG | + | chr2.4:15624039-15624058 | None:intergenic | 40.0% | |
| TTCAACCTCTATGTCTCAAG+TGG | - | chr2.4:15623525-15623544 | MS.gene94566:CDS | 40.0% | |
| TTCTTCCACTTGAGACATAG+AGG | + | chr2.4:15623533-15623552 | None:intergenic | 40.0% | |
| ! | CCTTATTTATCTGAGGCTTG+GGG | - | chr2.4:15623964-15623983 | MS.gene94566:CDS | 40.0% |
| !! | GCCTTATTTATCTGAGGCTT+GGG | - | chr2.4:15623963-15623982 | MS.gene94566:CDS | 40.0% |
| !! | GGATTGGTTTCTTTGATACC+AGG | - | chr2.4:15623895-15623914 | MS.gene94566:CDS | 40.0% |
| AAGCTGAAGCCAATTCTCTC+AGG | - | chr2.4:15623685-15623704 | MS.gene94566:CDS | 45.0% | |
| CCCCAAGCCTCAGATAAATA+AGG | + | chr2.4:15623967-15623986 | None:intergenic | 45.0% | |
| GGTACCCTCCAATTAAGCAA+TGG | + | chr2.4:15624018-15624037 | None:intergenic | 45.0% | |
| TGTGATGTGAGGACAGAGTT+TGG | + | chr2.4:15623664-15623683 | None:intergenic | 45.0% | |
| !! | AATTGGAGGGTACCAACTCT+TGG | - | chr2.4:15624024-15624043 | MS.gene94566:CDS | 45.0% |
| !! | GGCCTTATTTATCTGAGGCT+TGG | - | chr2.4:15623962-15623981 | MS.gene94566:CDS | 45.0% |
| !!! | TGTTGGTTTTTGCTCAACCC+TGG | + | chr2.4:15623916-15623935 | None:intergenic | 45.0% |
| GCTTCAGCTTCTGTGATGTG+AGG | + | chr2.4:15623675-15623694 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 15623412 | 15624113 | 15623412 | ID=MS.gene94566 |
| chr2.4 | mRNA | 15623412 | 15624113 | 15623412 | ID=MS.gene94566.t1;Parent=MS.gene94566 |
| chr2.4 | exon | 15623412 | 15624113 | 15623412 | ID=MS.gene94566.t1.exon1;Parent=MS.gene94566.t1 |
| chr2.4 | CDS | 15623412 | 15624113 | 15623412 | ID=cds.MS.gene94566.t1;Parent=MS.gene94566.t1 |
| Gene Sequence |
| Protein sequence |
