AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95227


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95227.t1 MTR_1g115455 74.274 241 29 2 2 242 3 210 3.89e-121 344
MS.gene95227.t1 MTR_8g445860 69.167 240 42 4 3 242 8 215 1.54e-107 310
MS.gene95227.t1 MTR_1g115440 56.327 245 70 5 2 242 5 216 5.60e-82 245
MS.gene95227.t1 MTR_0150s0010 59.259 243 66 5 2 242 4 215 2.19e-81 244
MS.gene95227.t1 MTR_8g044880 46.479 284 79 4 1 242 1 253 4.56e-70 216
MS.gene95227.t1 MTR_8g445910 46.182 275 75 4 1 233 1 244 1.43e-66 207
MS.gene95227.t1 MTR_1g115450 76.800 125 29 0 3 127 2 126 1.05e-63 195
MS.gene95227.t1 MTR_0150s0030 43.750 224 52 3 1 179 1 195 2.82e-47 155
MS.gene95227.t1 MTR_8g445920 71.084 83 24 0 2 84 4 86 5.31e-35 121
MS.gene95227.t1 MTR_8g044890 71.084 83 24 0 2 84 4 86 5.31e-35 121
MS.gene95227.t1 MTR_1g115435 34.274 248 91 9 3 242 2 185 1.66e-32 118
MS.gene95227.t1 MTR_1g115470 55.046 109 46 1 4 112 2 107 3.38e-31 119
MS.gene95227.t1 MTR_1g115470 39.850 133 48 4 112 242 181 283 4.97e-18 82.8
MS.gene95227.t1 MTR_7g012680 30.455 220 109 5 8 222 14 194 2.42e-24 100
MS.gene95227.t1 MTR_8g445870 41.935 124 44 4 3 121 2 102 1.77e-22 89.0
MS.gene95227.t1 MTR_7g066570 28.632 234 122 6 9 236 15 209 4.27e-22 95.1
MS.gene95227.t1 MTR_7g066340 27.049 244 135 6 2 240 6 211 1.11e-21 93.6
MS.gene95227.t1 MTR_7g058710 29.435 248 128 10 1 243 1 206 1.62e-20 90.1
MS.gene95227.t1 MTR_1g014060 29.060 234 116 9 18 243 10 201 3.60e-19 85.9
MS.gene95227.t1 MTR_7g066260 25.103 243 137 6 8 243 15 219 9.73e-19 85.1
MS.gene95227.t1 MTR_4g019310 29.183 257 134 9 1 243 1 223 5.17e-18 83.2
MS.gene95227.t1 MTR_7g066290 25.311 241 139 7 8 243 15 219 5.36e-18 82.8
MS.gene95227.t1 MTR_1g041315 27.686 242 129 8 8 242 14 216 1.48e-17 81.6
MS.gene95227.t1 MTR_7g066350 27.801 241 131 7 8 243 9 211 1.81e-17 81.3
MS.gene95227.t1 MTR_7g063900 26.693 251 135 9 1 243 1 210 2.44e-17 80.9
MS.gene95227.t1 MTR_7g063890 25.726 241 133 7 8 243 27 226 5.93e-17 80.1
MS.gene95227.t1 MTR_7g063880 27.888 251 127 9 2 243 5 210 1.36e-16 79.0
MS.gene95227.t1 MTR_7g063840 28.571 245 118 10 9 243 11 208 2.76e-16 77.8
MS.gene95227.t1 MTR_4g017830 28.745 247 125 10 9 243 13 220 3.56e-16 77.8
MS.gene95227.t1 MTR_4g017830 28.346 254 131 10 4 243 263 479 8.32e-16 76.6
MS.gene95227.t1 MTR_7g062280 31.405 242 133 10 8 243 10 224 3.77e-16 77.4
MS.gene95227.t1 MTR_4g017410 26.538 260 139 9 1 243 1 225 7.24e-16 76.6
MS.gene95227.t1 MTR_7g063660 27.530 247 130 8 3 243 4 207 8.11e-16 76.6
MS.gene95227.t1 MTR_4g019340 26.772 254 131 8 9 243 39 256 8.99e-16 76.6
MS.gene95227.t1 MTR_4g019340 39.241 79 46 1 8 86 303 379 2.95e-12 66.2
MS.gene95227.t1 MTR_7g066430 27.143 210 112 6 6 210 2 175 1.72e-15 75.5
MS.gene95227.t1 MTR_4g017610 27.935 247 125 9 11 243 16 223 1.76e-15 75.5
MS.gene95227.t1 MTR_2g029400 38.824 85 47 1 8 87 14 98 1.60e-14 72.4
MS.gene95227.t1 MTR_7g063820 30.263 228 112 11 3 221 2 191 2.21e-14 72.4
MS.gene95227.t1 MTR_4g017470 41.667 84 47 1 3 86 229 310 2.52e-14 72.0
MS.gene95227.t1 MTR_4g017470 32.110 109 59 1 6 99 10 118 4.94e-11 62.4
MS.gene95227.t1 MTR_7g062300 27.016 248 136 9 2 243 6 214 5.08e-14 71.2
MS.gene95227.t1 MTR_7g066360 23.077 247 146 6 2 243 8 215 1.30e-13 70.1
MS.gene95227.t1 MTR_7g066410 26.721 247 140 9 2 242 278 489 1.76e-13 69.7
MS.gene95227.t1 MTR_7g066410 28.365 208 110 9 8 210 10 183 2.84e-13 68.9
MS.gene95227.t1 MTR_4g017550 28.440 218 125 8 4 220 243 430 2.11e-13 69.3
MS.gene95227.t1 MTR_4g017440 24.231 260 138 8 2 243 8 226 3.98e-13 68.6
MS.gene95227.t1 MTR_4g017440 27.917 240 144 8 3 242 269 479 1.35e-12 67.0
MS.gene95227.t1 MTR_7g066310 33.333 117 65 3 8 117 7 117 8.66e-13 67.4
MS.gene95227.t1 MTR_3g007740 26.531 245 112 8 8 238 9 199 1.07e-12 67.4
MS.gene95227.t1 MTR_7g066580 32.743 113 64 4 14 120 1 107 1.62e-12 66.6
MS.gene95227.t1 MTR_7g063830 32.450 151 85 6 1 138 1 147 2.81e-12 66.2
MS.gene95227.t1 MTR_7g066490 40.909 88 47 1 4 86 12 99 4.54e-12 65.5
MS.gene95227.t1 MTR_7g066480 27.642 246 121 10 8 243 16 214 5.45e-12 65.1
MS.gene95227.t1 MTR_7g063920 37.778 90 51 1 2 86 5 94 2.96e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95227.t1 AT3G09110 28.112 249 119 6 8 243 9 210 5.31e-20 87.8
MS.gene95227.t1 AT5G01130 28.340 247 126 6 3 243 4 205 1.90e-18 84.3
MS.gene95227.t1 AT5G01120 25.911 247 132 7 9 243 10 217 8.94e-18 82.4
MS.gene95227.t1 AT3G09140 26.295 251 149 6 3 243 4 228 7.55e-17 79.7
MS.gene95227.t1 AT3G09140 26.295 251 149 6 3 243 4 228 7.74e-17 79.7
MS.gene95227.t1 AT5G43240 26.613 248 132 8 9 243 10 220 3.90e-16 77.4
MS.gene95227.t1 AT5G43240 26.613 248 132 8 9 243 10 220 3.90e-16 77.4
MS.gene95227.t1 AT5G43240 26.613 248 132 8 9 243 10 220 3.90e-16 77.4
MS.gene95227.t1 AT5G01150 26.016 246 141 5 3 243 4 213 1.76e-14 72.8
MS.gene95227.t1 AT3G09120 25.820 244 145 6 4 243 2 213 1.07e-11 63.9

Find 55 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAGATGTTATCACATTTA+TGG 0.180834 3.2:+14102912 MS.gene95227:CDS
GACCGTGGTTCTCTTCCTTT+TGG 0.187396 3.2:+14102748 MS.gene95227:CDS
CATCATCAATGTCTTCATTC+TGG 0.201655 3.2:-14102630 None:intergenic
TTTGGGGCTAGTCCTGCTTT+TGG 0.253034 3.2:+14102784 MS.gene95227:CDS
TTTGGGGCTAGTCCTGCTTT+TGG 0.267199 3.2:+14102766 MS.gene95227:CDS
AATGGAATGGTTGGTAGTTT+AGG 0.275746 3.2:+14102487 MS.gene95227:CDS
GTGGAAGAGCTTTGAGCTCT+TGG 0.285273 3.2:-14102586 None:intergenic
CACAAACCCACTCTTAATAT+CGG 0.339765 3.2:-14102890 None:intergenic
AAGTTCGTTATGTCGGAAAA+AGG 0.346729 3.2:+14102845 MS.gene95227:CDS
ATTTATGGTGATGGATGATT+TGG 0.351782 3.2:+14102927 MS.gene95227:CDS
AGGACATCCTTAGTTTGGTT+TGG 0.370323 3.2:-14102556 None:intergenic
CAACAGTGTGATGCTTGATA+TGG 0.370669 3.2:-14102968 None:intergenic
TTAGCAGGACATCCTTAGTT+TGG 0.377274 3.2:-14102561 None:intergenic
TGGAAGAGCTTTGAGCTCTT+GGG 0.383995 3.2:-14102585 None:intergenic
ATCATCAATGTCTTCATTCT+GGG 0.389042 3.2:-14102629 None:intergenic
ATAGTCTTCGAGCAGTATAC+TGG 0.410060 3.2:-14102814 None:intergenic
CAACCATTCCATTTGTGCTT+AGG 0.411279 3.2:-14102477 None:intergenic
AGGAAGAGAACCACGGTCAT+TGG 0.418470 3.2:-14102743 None:intergenic
TTTGAGCTCTTGGGTTTAGC+AGG 0.423021 3.2:-14102576 None:intergenic
GATTGAAACCTTGCAAGAAA+AGG 0.450000 3.2:+14103011 MS.gene95227:CDS
AGCTTAACCACGGTGCCAAT+TGG 0.456102 3.2:-14102454 None:intergenic
CAAGAAAAGGTTGTTGAGAT+GGG 0.463384 3.2:+14103024 MS.gene95227:CDS
AGCTCTTCCACTGAAATCTC+AGG 0.471238 3.2:+14102598 MS.gene95227:CDS
GTTAAGCTCCTAAGCACAAA+TGG 0.472625 3.2:+14102469 MS.gene95227:CDS
GCAAGAAAAGGTTGTTGAGA+TGG 0.482167 3.2:+14103023 MS.gene95227:CDS
CAATGTCTTCATTCTGGGTA+AGG 0.483882 3.2:-14102624 None:intergenic
ACCACGGTCATTGGCAGACC+TGG 0.490771 3.2:-14102734 None:intergenic
AATTTGCTATGCTTGCCAAT+TGG 0.494602 3.2:+14102439 MS.gene95227:CDS
AAGAAGTTTCAGTGACACTT+TGG 0.494769 3.2:-14102350 None:intergenic
ACCTCAAAGTCACACTTACC+TGG 0.508917 3.2:-14102682 None:intergenic
AACAGTGAAGTTCGTTATGT+CGG 0.513095 3.2:+14102838 MS.gene95227:CDS
TGTTATCACATTTATGGTGA+TGG 0.515514 3.2:+14102918 MS.gene95227:CDS
GGTTTGGTTGCATGTAACTC+TGG 0.520873 3.2:-14102540 None:intergenic
TCCAGGTAAGTGTGACTTTG+AGG 0.521666 3.2:+14102681 MS.gene95227:CDS
AAAGCAGGACTAGCCCCAAA+AGG 0.523257 3.2:-14102763 None:intergenic
GCAGGACTAGCCCCAAAAGC+AGG 0.523795 3.2:-14102778 None:intergenic
CATGCAACCAAACCAAACTA+AGG 0.523850 3.2:+14102549 MS.gene95227:CDS
CTAAGCACAAATGGAATGGT+TGG 0.538348 3.2:+14102478 MS.gene95227:CDS
ACACCACTCGTTGTACTGCC+AGG 0.539159 3.2:+14102716 MS.gene95227:CDS
AGAGTTTCCGATATTAAGAG+TGG 0.573501 3.2:+14102883 MS.gene95227:CDS
AAGTTTCAGTGACACTTTGG+TGG 0.576601 3.2:-14102347 None:intergenic
GCTCCTAAGCACAAATGGAA+TGG 0.578156 3.2:+14102474 MS.gene95227:CDS
ATGCTTGATATGGTTGACAT+TGG 0.584309 3.2:-14102958 None:intergenic
ACTGGACTAGCCCCAAAAGC+AGG 0.584508 3.2:-14102796 None:intergenic
GACATTGATGATGAAGATGA+AGG 0.587367 3.2:+14102640 MS.gene95227:CDS
TGTTGAGATGGGAATGGATG+AGG 0.588340 3.2:+14103035 MS.gene95227:CDS
ACTAAGTTATACATGTGTCC+AGG 0.594908 3.2:+14102664 MS.gene95227:CDS
GAGTTTCCGATATTAAGAGT+GGG 0.614629 3.2:+14102884 MS.gene95227:CDS
AAAGGTTGTTGAGATGGGAA+TGG 0.615414 3.2:+14103029 MS.gene95227:CDS
GCCAGGTCTGCCAATGACCG+TGG 0.633912 3.2:+14102733 MS.gene95227:CDS
AAGGAAGCCTGAGATTTCAG+TGG 0.655993 3.2:-14102605 None:intergenic
TGTGCTTAGGAGCTTAACCA+CGG 0.668356 3.2:-14102464 None:intergenic
ATGCTTGCCAATTGGCACCG+TGG 0.701156 3.2:+14102447 MS.gene95227:CDS
AGACCTGGCAGTACAACGAG+TGG 0.704338 3.2:-14102719 None:intergenic
CCCCAAAAGGAAGAGAACCA+CGG 0.716251 3.2:-14102750 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GAAAGATGTTATCACATTTA+TGG + chr3.2:14102912-14102931 MS.gene95227:CDS 25.0%
ATCATCAATGTCTTCATTCT+GGG - chr3.2:14102632-14102651 None:intergenic 30.0%
TGTTATCACATTTATGGTGA+TGG + chr3.2:14102918-14102937 MS.gene95227:CDS 30.0%
!! ATTTATGGTGATGGATGATT+TGG + chr3.2:14102927-14102946 MS.gene95227:CDS 30.0%
AACAGTGAAGTTCGTTATGT+CGG + chr3.2:14102838-14102857 MS.gene95227:CDS 35.0%
AAGAAGTTTCAGTGACACTT+TGG - chr3.2:14102353-14102372 None:intergenic 35.0%
AAGTTCGTTATGTCGGAAAA+AGG + chr3.2:14102845-14102864 MS.gene95227:CDS 35.0%
ACTAAGTTATACATGTGTCC+AGG + chr3.2:14102664-14102683 MS.gene95227:CDS 35.0%
AGAGTTTCCGATATTAAGAG+TGG + chr3.2:14102883-14102902 MS.gene95227:CDS 35.0%
ATGCTTGATATGGTTGACAT+TGG - chr3.2:14102961-14102980 None:intergenic 35.0%
CAAGAAAAGGTTGTTGAGAT+GGG + chr3.2:14103024-14103043 MS.gene95227:CDS 35.0%
CACAAACCCACTCTTAATAT+CGG - chr3.2:14102893-14102912 None:intergenic 35.0%
CATCATCAATGTCTTCATTC+TGG - chr3.2:14102633-14102652 None:intergenic 35.0%
GAGTTTCCGATATTAAGAGT+GGG + chr3.2:14102884-14102903 MS.gene95227:CDS 35.0%
GATTGAAACCTTGCAAGAAA+AGG + chr3.2:14103011-14103030 MS.gene95227:CDS 35.0%
!! AATGGAATGGTTGGTAGTTT+AGG + chr3.2:14102487-14102506 MS.gene95227:CDS 35.0%
!! AATTTGCTATGCTTGCCAAT+TGG + chr3.2:14102439-14102458 MS.gene95227:CDS 35.0%
!! GACATTGATGATGAAGATGA+AGG + chr3.2:14102640-14102659 MS.gene95227:CDS 35.0%
AAAGGTTGTTGAGATGGGAA+TGG + chr3.2:14103029-14103048 MS.gene95227:CDS 40.0%
AAGTTTCAGTGACACTTTGG+TGG - chr3.2:14102350-14102369 None:intergenic 40.0%
ATAGTCTTCGAGCAGTATAC+TGG - chr3.2:14102817-14102836 None:intergenic 40.0%
CAACAGTGTGATGCTTGATA+TGG - chr3.2:14102971-14102990 None:intergenic 40.0%
CAACCATTCCATTTGTGCTT+AGG - chr3.2:14102480-14102499 None:intergenic 40.0%
CAATGTCTTCATTCTGGGTA+AGG - chr3.2:14102627-14102646 None:intergenic 40.0%
CATGCAACCAAACCAAACTA+AGG + chr3.2:14102549-14102568 MS.gene95227:CDS 40.0%
CTAAGCACAAATGGAATGGT+TGG + chr3.2:14102478-14102497 MS.gene95227:CDS 40.0%
GCAAGAAAAGGTTGTTGAGA+TGG + chr3.2:14103023-14103042 MS.gene95227:CDS 40.0%
GTTAAGCTCCTAAGCACAAA+TGG + chr3.2:14102469-14102488 MS.gene95227:CDS 40.0%
TCGTTATGTCGGAAAAAGGA+AGG + chr3.2:14102849-14102868 MS.gene95227:CDS 40.0%
TTAGCAGGACATCCTTAGTT+TGG - chr3.2:14102564-14102583 None:intergenic 40.0%
! AGGACATCCTTAGTTTGGTT+TGG - chr3.2:14102559-14102578 None:intergenic 40.0%
! CTCAACAACCTTTTCTTGCA+AGG - chr3.2:14103022-14103041 None:intergenic 40.0%
AAGGAAGCCTGAGATTTCAG+TGG - chr3.2:14102608-14102627 None:intergenic 45.0%
ACCTCAAAGTCACACTTACC+TGG - chr3.2:14102685-14102704 None:intergenic 45.0%
AGCTCTTCCACTGAAATCTC+AGG + chr3.2:14102598-14102617 MS.gene95227:CDS 45.0%
GCTCCTAAGCACAAATGGAA+TGG + chr3.2:14102474-14102493 MS.gene95227:CDS 45.0%
TCCAGGTAAGTGTGACTTTG+AGG + chr3.2:14102681-14102700 MS.gene95227:CDS 45.0%
TGTGCTTAGGAGCTTAACCA+CGG - chr3.2:14102467-14102486 None:intergenic 45.0%
TGTTGAGATGGGAATGGATG+AGG + chr3.2:14103035-14103054 MS.gene95227:CDS 45.0%
TTTGAGCTCTTGGGTTTAGC+AGG - chr3.2:14102579-14102598 None:intergenic 45.0%
! ACCGTGGTTCTCTTCCTTTT+GGG + chr3.2:14102749-14102768 MS.gene95227:CDS 45.0%
! GGTTTGGTTGCATGTAACTC+TGG - chr3.2:14102543-14102562 None:intergenic 45.0%
!! TGGAAGAGCTTTGAGCTCTT+GGG - chr3.2:14102588-14102607 None:intergenic 45.0%
AGGAAGAGAACCACGGTCAT+TGG - chr3.2:14102746-14102765 None:intergenic 50.0%
CCCCAAAAGGAAGAGAACCA+CGG - chr3.2:14102753-14102772 None:intergenic 50.0%
! AGCTTAACCACGGTGCCAAT+TGG - chr3.2:14102457-14102476 None:intergenic 50.0%
! CCGTGGTTCTCTTCCTTTTG+GGG + chr3.2:14102750-14102769 MS.gene95227:CDS 50.0%
! GACCGTGGTTCTCTTCCTTT+TGG + chr3.2:14102748-14102767 MS.gene95227:CDS 50.0%
!! AAAGCAGGACTAGCCCCAAA+AGG - chr3.2:14102766-14102785 None:intergenic 50.0%
!! GTGGAAGAGCTTTGAGCTCT+TGG - chr3.2:14102589-14102608 None:intergenic 50.0%
!!! TTGGGGCTAGTCCTGCTTTT+GGG + chr3.2:14102767-14102786 MS.gene95227:CDS 50.0%
!!! TTTGGGGCTAGTCCTGCTTT+TGG + chr3.2:14102766-14102785 MS.gene95227:CDS 50.0%
ACACCACTCGTTGTACTGCC+AGG + chr3.2:14102716-14102735 MS.gene95227:CDS 55.0%
AGACCTGGCAGTACAACGAG+TGG - chr3.2:14102722-14102741 None:intergenic 55.0%
ATGCTTGCCAATTGGCACCG+TGG + chr3.2:14102447-14102466 MS.gene95227:CDS 55.0%
!! ACTGGACTAGCCCCAAAAGC+AGG - chr3.2:14102799-14102818 None:intergenic 55.0%
!!! TGGGGCTAGTCCTGCTTTTG+GGG + chr3.2:14102768-14102787 MS.gene95227:CDS 55.0%
ACCACGGTCATTGGCAGACC+TGG - chr3.2:14102737-14102756 None:intergenic 60.0%
!! GCAGGACTAGCCCCAAAAGC+AGG - chr3.2:14102781-14102800 None:intergenic 60.0%
GCCAGGTCTGCCAATGACCG+TGG + chr3.2:14102733-14102752 MS.gene95227:CDS 65.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 14102331 14103065 14102331 ID=MS.gene95227
chr3.2 mRNA 14102331 14103065 14102331 ID=MS.gene95227.t1;Parent=MS.gene95227
chr3.2 exon 14102331 14103065 14102331 ID=MS.gene95227.t1.exon1;Parent=MS.gene95227.t1
chr3.2 CDS 14102331 14103065 14102331 ID=cds.MS.gene95227.t1;Parent=MS.gene95227.t1
Gene Sequence

>MS.gene95227

ATGTCTTCTTCTTCTACCACCAAAGTGTCACTGAAACTTCTTATAGATACAAAGAATGAGAGAGTTCTCTTTGCTGAAGCTTCAAAATCTGTAATAGACTTTCTGTTCAATTTGCTATGCTTGCCAATTGGCACCGTGGTTAAGCTCCTAAGCACAAATGGAATGGTTGGTAGTTTAGGAAATCTGTATGAGAGTGTTGAAAACTTCGACCAGAGTTACATGCAACCAAACCAAACTAAGGATGTCCTGCTAAACCCAAGAGCTCAAAGCTCTTCCACTGAAATCTCAGGCTTCCTTACCCAGAATGAAGACATTGATGATGAAGATGAAGGAACTAAGTTATACATGTGTCCAGGTAAGTGTGACTTTGAGGTAACAAATGATAACACCACTCGTTGTACTGCCAGGTCTGCCAATGACCGTGGTTCTCTTCCTTTTGGGGCTAGTCCTGCTTTTGGGGCTAGTCCTGCTTTTGGGGCTAGTCCAGTATACTGCTCGAAGACTATGAACAGTGAAGTTCGTTATGTCGGAAAAAGGAAGGTTGCTAAAAACAGAGTTTCCGATATTAAGAGTGGGTTTGTGAAAGATGTTATCACATTTATGGTGATGGATGATTTGGTGATTCAGCCAATGTCAACCATATCAAGCATCACACTGTTGAACAAGTTCAAAGTGAAAGAGATTGAAACCTTGCAAGAAAAGGTTGTTGAGATGGGAATGGATGAGGTAAATTAA

Protein sequence

>MS.gene95227.t1

MSSSSTTKVSLKLLIDTKNERVLFAEASKSVIDFLFNLLCLPIGTVVKLLSTNGMVGSLGNLYESVENFDQSYMQPNQTKDVLLNPRAQSSSTEISGFLTQNEDIDDEDEGTKLYMCPGKCDFEVTNDNTTRCTARSANDRGSLPFGASPAFGASPAFGASPVYCSKTMNSEVRYVGKRKVAKNRVSDIKSGFVKDVITFMVMDDLVIQPMSTISSITLLNKFKVKEIETLQEKVVEMGMDEVN