Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95474.t1 | RHN55581.1 | 93.2 | 59 | 3 | 1 | 1 | 58 | 158 | 216 | 8.80E-21 | 109 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95474.t1 | G7JY28 | 93.2 | 59 | 3 | 1 | 1 | 58 | 228 | 286 | 6.3e-21 | 109.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054708 | MS.gene95474 | 0.816304 | 5.92E-52 | -1.69E-46 |
| MS.gene058628 | MS.gene95474 | 0.806999 | 6.26E-50 | -1.69E-46 |
| MS.gene058629 | MS.gene95474 | 0.802497 | 5.46E-49 | -1.69E-46 |
| MS.gene058630 | MS.gene95474 | 0.804258 | 2.36E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95474.t1 | MTR_5g045130 | 93.220 | 59 | 3 | 1 | 1 | 58 | 228 | 286 | 2.32e-30 | 110 |
| MS.gene95474.t1 | MTR_5g045160 | 94.915 | 59 | 2 | 1 | 1 | 58 | 393 | 451 | 5.65e-30 | 110 |
| MS.gene95474.t1 | MTR_3g103410 | 86.441 | 59 | 7 | 1 | 1 | 58 | 425 | 483 | 1.07e-27 | 103 |
| MS.gene95474.t1 | MTR_6g445440 | 79.661 | 59 | 11 | 1 | 1 | 58 | 365 | 423 | 3.05e-25 | 96.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95474.t1 | AT5G13210 | 70.492 | 61 | 17 | 1 | 1 | 60 | 382 | 442 | 1.82e-22 | 89.0 |
| MS.gene95474.t1 | AT3G24780 | 67.797 | 59 | 18 | 1 | 1 | 58 | 434 | 492 | 4.34e-19 | 79.3 |
| MS.gene95474.t1 | AT5G43400 | 61.905 | 63 | 19 | 1 | 1 | 58 | 357 | 419 | 6.63e-18 | 75.9 |
| MS.gene95474.t1 | AT5G43390 | 60.000 | 60 | 22 | 2 | 1 | 58 | 348 | 407 | 8.20e-17 | 72.8 |
Find 15 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCTCCTTATAAAACTTCA+TGG | 0.297463 | 5.2:+35447208 | None:intergenic |
| GGAGAGGTTTGAGAAGTACT+TGG | 0.389397 | 5.2:-35447168 | MS.gene95474:CDS |
| TACTTGGAGGATGTGAAGGC+AGG | 0.488941 | 5.2:-35447152 | MS.gene95474:CDS |
| CGTTGATGATTTGCTCAAGA+AGG | 0.491296 | 5.2:-35447031 | MS.gene95474:CDS |
| ATTATAGAGTCTTTGCGTGA+TGG | 0.519156 | 5.2:-35447089 | MS.gene95474:CDS |
| GAGGTTTGAGAAGTACTTGG+AGG | 0.521875 | 5.2:-35447165 | MS.gene95474:CDS |
| GTTGATGATTTGCTCAAGAA+GGG | 0.552807 | 5.2:-35447030 | MS.gene95474:CDS |
| GGGAAGACTACTATTGCTGC+TGG | 0.579975 | 5.2:-35447131 | MS.gene95474:CDS |
| GAAGTACTTGGAGGATGTGA+AGG | 0.604063 | 5.2:-35447156 | MS.gene95474:CDS |
| ACTTGGAGGATGTGAAGGCA+GGG | 0.604217 | 5.2:-35447151 | MS.gene95474:CDS |
| GTGAAGTGGCTGAGTTGCAG+TGG | 0.607162 | 5.2:-35447061 | MS.gene95474:CDS |
| GAGTCTTTGCGTGATGGAGA+TGG | 0.610505 | 5.2:-35447083 | MS.gene95474:CDS |
| TGCTCAAGAAGGGAAAGATG+AGG | 0.619614 | 5.2:-35447020 | None:intergenic |
| GCGTGATGGAGATGGTGAAG+TGG | 0.668727 | 5.2:-35447075 | MS.gene95474:CDS |
| AAAGACTCTATAATCTCGTG+AGG | 0.670125 | 5.2:+35447098 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAAGTTTTTGAAGCATGATA+AGG | - | chr5.2:35447039-35447058 | MS.gene95474:CDS | 30.0% |
| AAAGACTCTATAATCTCGTG+AGG | + | chr5.2:35447133-35447152 | None:intergenic | 35.0% | |
| ATTATAGAGTCTTTGCGTGA+TGG | - | chr5.2:35447139-35447158 | MS.gene95474:CDS | 35.0% | |
| !! | GTTGATGATTTGCTCAAGAA+GGG | - | chr5.2:35447198-35447217 | MS.gene95474:CDS | 35.0% |
| !!! | TTTTGAAGCATGATAAGGAG+AGG | - | chr5.2:35447044-35447063 | MS.gene95474:CDS | 35.0% |
| !! | CGTTGATGATTTGCTCAAGA+AGG | - | chr5.2:35447197-35447216 | MS.gene95474:CDS | 40.0% |
| ! | GAAGTACTTGGAGGATGTGA+AGG | - | chr5.2:35447072-35447091 | MS.gene95474:CDS | 45.0% |
| !! | GAGGTTTGAGAAGTACTTGG+AGG | - | chr5.2:35447063-35447082 | MS.gene95474:CDS | 45.0% |
| !! | GGAGAGGTTTGAGAAGTACT+TGG | - | chr5.2:35447060-35447079 | MS.gene95474:CDS | 45.0% |
| ACTTGGAGGATGTGAAGGCA+GGG | - | chr5.2:35447077-35447096 | MS.gene95474:CDS | 50.0% | |
| GGGAAGACTACTATTGCTGC+TGG | - | chr5.2:35447097-35447116 | MS.gene95474:CDS | 50.0% | |
| TACTTGGAGGATGTGAAGGC+AGG | - | chr5.2:35447076-35447095 | MS.gene95474:CDS | 50.0% | |
| ! | GAGTCTTTGCGTGATGGAGA+TGG | - | chr5.2:35447145-35447164 | MS.gene95474:CDS | 50.0% |
| GTGAAGTGGCTGAGTTGCAG+TGG | - | chr5.2:35447167-35447186 | MS.gene95474:CDS | 55.0% | |
| ! | GCGTGATGGAGATGGTGAAG+TGG | - | chr5.2:35447153-35447172 | MS.gene95474:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 35447022 | 35447228 | 35447022 | ID=MS.gene95474 |
| chr5.2 | mRNA | 35447022 | 35447228 | 35447022 | ID=MS.gene95474.t1;Parent=MS.gene95474 |
| chr5.2 | exon | 35447022 | 35447228 | 35447022 | ID=MS.gene95474.t1.exon1;Parent=MS.gene95474.t1 |
| chr5.2 | CDS | 35447022 | 35447228 | 35447022 | ID=cds.MS.gene95474.t1;Parent=MS.gene95474.t1 |
| Gene Sequence |
| Protein sequence |
