Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95742.t1 | XP_003605731.2 | 97.7 | 613 | 14 | 0 | 1 | 613 | 22 | 634 | 0.00E+00 | 1241.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95742.t1 | Q5MAU8 | 35.6 | 616 | 338 | 15 | 16 | 611 | 13 | 589 | 6.0e-101 | 369.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95742.t1 | G7JSI6 | 97.7 | 613 | 14 | 0 | 1 | 613 | 22 | 634 | 0.0e+00 | 1241.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04991 | MS.gene95742 | -0.804965 | 1.68E-49 | -1.69E-46 |
| MS.gene055505 | MS.gene95742 | 0.81991 | 9.06E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 122 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTCATTTCCTTTGGTGATA+TGG | 0.160418 | 4.1:-54435649 | MS.gene95742:CDS |
| CTGATAATGCAGTAAAATTT+TGG | 0.174680 | 4.1:+54440900 | None:intergenic |
| CCTAGTCCTGCCAAGGATTT+TGG | 0.184357 | 4.1:-54436054 | MS.gene95742:CDS |
| AATCCTAAGAAGCCACTTTA+TGG | 0.194096 | 4.1:-54436860 | MS.gene95742:CDS |
| AGCTGGTGGAGTTGAAAATT+TGG | 0.227346 | 4.1:+54435692 | None:intergenic |
| TGTCCATAAAGTGGCTTCTT+AGG | 0.235315 | 4.1:+54436857 | None:intergenic |
| TTCAGAGATTATGTCGATTC+TGG | 0.253362 | 4.1:-54435029 | MS.gene95742:intron |
| TAGAAATGATTTAAGCTTAG+AGG | 0.258127 | 4.1:-54433483 | MS.gene95742:intron |
| GCCATTGCTGTTACAGAATA+AGG | 0.264422 | 4.1:-54434341 | MS.gene95742:intron |
| TTCATTTCCTTTGGTGATAT+GGG | 0.313819 | 4.1:-54435648 | MS.gene95742:CDS |
| CAGCTCAAATTCTAAATTGT+TGG | 0.325166 | 4.1:-54440807 | MS.gene95742:CDS |
| GCAGTATAACTGGATGAAAA+AGG | 0.337674 | 4.1:-54434748 | MS.gene95742:intron |
| GAGCTCAAATTCATTTCCTT+TGG | 0.349534 | 4.1:-54435657 | MS.gene95742:CDS |
| CGAAACCGAGAGATGGAGAT+TGG | 0.349856 | 4.1:-54440748 | MS.gene95742:CDS |
| TTGAAAAGGATTTCTGAGTT+TGG | 0.356122 | 4.1:-54433527 | MS.gene95742:CDS |
| AACTTTCCCTTCCTCAACTT+TGG | 0.356583 | 4.1:-54440954 | MS.gene95742:CDS |
| TTTCTCAGCCCCTGCCTTGT+TGG | 0.369987 | 4.1:-54436908 | MS.gene95742:CDS |
| GGCTTATCCTTTGCTGCAGT+TGG | 0.372410 | 4.1:+54434939 | None:intergenic |
| TTGAAGCTACTGGACTAATA+AGG | 0.387415 | 4.1:+54435193 | None:intergenic |
| TATTCTTACTTGGAATTTGA+AGG | 0.390234 | 4.1:+54440710 | None:intergenic |
| GAGTTCTATTATTTACAAAC+TGG | 0.393218 | 4.1:-54440573 | MS.gene95742:CDS |
| TCCTTTGCTGCAGTTGGCAT+TGG | 0.393804 | 4.1:+54434945 | None:intergenic |
| ATTGTTTGAGAATTTGTCTA+AGG | 0.400148 | 4.1:+54434069 | None:intergenic |
| ATCAGATACAAGAGAAGTTA+AGG | 0.412947 | 4.1:-54433331 | MS.gene95742:CDS |
| CATGCTGTCATTGGCATGGC+TGG | 0.413111 | 4.1:-54434097 | MS.gene95742:CDS |
| TTCCTCAACTTTGGACCTTA+CGG | 0.414650 | 4.1:-54440945 | MS.gene95742:CDS |
| AGTGCTTACTCCTACAGATA+TGG | 0.417269 | 4.1:-54435976 | MS.gene95742:CDS |
| CTGTAGGAGTAAGCACTTGA+TGG | 0.420678 | 4.1:+54435982 | None:intergenic |
| TCAGCCCCTGCCTTGTTGGA+AGG | 0.422865 | 4.1:-54436904 | MS.gene95742:CDS |
| CATTCCTATAAAAGATCAAA+AGG | 0.427448 | 4.1:-54434161 | MS.gene95742:CDS |
| TTTAGATGATAATCCGTTGT+TGG | 0.430584 | 4.1:+54434390 | None:intergenic |
| AAACACTGAGTTTACATTGT+TGG | 0.436214 | 4.1:+54435275 | None:intergenic |
| CTGATATTCAGTTTAAGATA+AGG | 0.443765 | 4.1:+54440827 | None:intergenic |
| GGAGACATAAGTTATGCAAC+TGG | 0.453721 | 4.1:-54435246 | MS.gene95742:CDS |
| TATGAAACTCTTGAAGCTAC+TGG | 0.454406 | 4.1:+54435183 | None:intergenic |
| GTTTCATACATGACAGCAAT+CGG | 0.454768 | 4.1:-54435168 | MS.gene95742:CDS |
| GGTCTAGTAACTCGGCTGAT+TGG | 0.457957 | 4.1:-54435725 | MS.gene95742:intron |
| CAGCAAATCCAATATGCAAA+TGG | 0.463555 | 4.1:-54436249 | MS.gene95742:CDS |
| TTTGTCACGGTTACCGTCAC+CGG | 0.464369 | 4.1:-54440776 | MS.gene95742:CDS |
| ATAGAGTTTGTGTTCTTCAC+TGG | 0.469598 | 4.1:-54436935 | MS.gene95742:CDS |
| TTGATAACCGATAGTGCTCC+AGG | 0.477995 | 4.1:+54435321 | None:intergenic |
| CTGAGAGCTGGAGCTTGAAA+AGG | 0.484148 | 4.1:-54433541 | MS.gene95742:CDS |
| AGCTCATCTGATCCTCCAGC+TGG | 0.484295 | 4.1:+54435675 | None:intergenic |
| CCAAAATCCTTGGCAGGACT+AGG | 0.485338 | 4.1:+54436054 | None:intergenic |
| TTCAGAAGAACACTACATTC+AGG | 0.496934 | 4.1:-54435607 | MS.gene95742:intron |
| CCACATTCACCACCAGAGTC+AGG | 0.499819 | 4.1:+54434990 | None:intergenic |
| AACCGTAAGGTCCAAAGTTG+AGG | 0.499979 | 4.1:+54440943 | None:intergenic |
| GCTCCTGTACATGCTGTCAT+TGG | 0.501222 | 4.1:-54434106 | MS.gene95742:CDS |
| GTTTATGTTACTCCTGACTC+TGG | 0.501264 | 4.1:-54435002 | MS.gene95742:CDS |
| AATGCAAGAAAGAACAGAAT+GGG | 0.502167 | 4.1:-54437021 | MS.gene95742:CDS |
| ATGAGATTGACATGGGTTAG+TGG | 0.502611 | 4.1:-54436285 | MS.gene95742:CDS |
| TGCATTGGCAGGCTGAGAGC+TGG | 0.506342 | 4.1:-54433553 | MS.gene95742:intron |
| AGTGGACCTTCCAACAAGGC+AGG | 0.508899 | 4.1:+54436898 | None:intergenic |
| ATTTAGTGTGTTTAACCGTA+AGG | 0.512631 | 4.1:+54440930 | None:intergenic |
| TGAGATTGACATGGGTTAGT+GGG | 0.513254 | 4.1:-54436284 | MS.gene95742:CDS |
| GAGTTTGTGTTCTTCACTGG+TGG | 0.514565 | 4.1:-54436932 | MS.gene95742:CDS |
| TGCCATCCAAAATCCTTGGC+AGG | 0.514716 | 4.1:+54436048 | None:intergenic |
| GGGAAAGTTGTAGACATTGA+AGG | 0.519414 | 4.1:+54440968 | None:intergenic |
| CCTGACTCTGGTGGTGAATG+TGG | 0.520849 | 4.1:-54434990 | MS.gene95742:CDS |
| TGAACAGCCTGGAGCACTAT+CGG | 0.522454 | 4.1:-54435328 | MS.gene95742:intron |
| AGTGCTGAATGGATGAATCC+TGG | 0.523299 | 4.1:+54436021 | None:intergenic |
| TCCAATGCCAACTGCAGCAA+AGG | 0.523966 | 4.1:-54434946 | MS.gene95742:CDS |
| TTAATAGACAACATACTCCT+TGG | 0.526063 | 4.1:-54434713 | MS.gene95742:CDS |
| ACCGAGAGATGGAGATTGGT+TGG | 0.528220 | 4.1:-54440744 | MS.gene95742:CDS |
| CTTGAGATATGTCCATAAAG+TGG | 0.530030 | 4.1:+54436848 | None:intergenic |
| GACATACCTTGACAGGGTAA+TGG | 0.531072 | 4.1:+54440523 | None:intergenic |
| GCCTTATTCTGTAACAGCAA+TGG | 0.531563 | 4.1:+54434340 | None:intergenic |
| ATCCATTCAGCACTTATGAC+AGG | 0.531954 | 4.1:-54436012 | MS.gene95742:CDS |
| TAATATCAACCGAACATGAC+TGG | 0.532916 | 4.1:-54434878 | MS.gene95742:CDS |
| ATGCCAATGACAGCATGTAC+AGG | 0.534351 | 4.1:+54434103 | None:intergenic |
| ATCATGCCATCCAAAATCCT+TGG | 0.535399 | 4.1:+54436044 | None:intergenic |
| TTCATACCTTCCATATCTGT+AGG | 0.537773 | 4.1:+54435966 | None:intergenic |
| TCATCTGATCCTCCAGCTGG+TGG | 0.542127 | 4.1:+54435678 | None:intergenic |
| AGTTGTGCCTAGTCCTGCCA+AGG | 0.542146 | 4.1:-54436061 | MS.gene95742:CDS |
| CTGGTCCGAAAATTCTGAGC+AGG | 0.542863 | 4.1:-54434859 | MS.gene95742:intron |
| AAATTCTAAATTGTTGGATG+AGG | 0.543261 | 4.1:-54440801 | MS.gene95742:CDS |
| ATTGGAAAGTATGTTTCATA+CGG | 0.543798 | 4.1:+54434963 | None:intergenic |
| GCCAACCAATCTCCATCTCT+CGG | 0.544480 | 4.1:+54440743 | None:intergenic |
| GAATGCAAGAAAGAACAGAA+TGG | 0.545134 | 4.1:-54437022 | MS.gene95742:CDS |
| GCTTTGCCATTACCCTGTCA+AGG | 0.547055 | 4.1:-54440529 | MS.gene95742:intron |
| TGTGCATGACATACCTTGAC+AGG | 0.552123 | 4.1:+54440516 | None:intergenic |
| TTTGCATATTGGATTTGCTG+AGG | 0.552556 | 4.1:+54436252 | None:intergenic |
| GGCGTTTCGAAACCGAGAGA+TGG | 0.556098 | 4.1:-54440755 | MS.gene95742:CDS |
| TTTCATACATGACAGCAATC+GGG | 0.557285 | 4.1:-54435167 | MS.gene95742:CDS |
| CCAATGATGGTCTAGTAACT+CGG | 0.560138 | 4.1:-54435733 | MS.gene95742:intron |
| CGGTTTCGAAACGCCGGTGA+CGG | 0.566494 | 4.1:+54440763 | None:intergenic |
| TGGACCTTCCAACAAGGCAG+GGG | 0.567412 | 4.1:+54436900 | None:intergenic |
| TTAGGATTAGCAAAACTCAA+AGG | 0.568493 | 4.1:+54436875 | None:intergenic |
| CCTTGGTACTCTATTGAACA+AGG | 0.571416 | 4.1:-54434918 | MS.gene95742:CDS |
| GGAAAGTTGTAGACATTGAA+GGG | 0.572421 | 4.1:+54440969 | None:intergenic |
| CCGTTGTTGGAAGTGTACAT+TGG | 0.575888 | 4.1:+54434403 | None:intergenic |
| TGTACATGCTGTCATTGGCA+TGG | 0.577657 | 4.1:-54434101 | MS.gene95742:CDS |
| GTCCTGTCATAAGTGCTGAA+TGG | 0.579771 | 4.1:+54436010 | None:intergenic |
| ATTCCTATAAAAGATCAAAA+GGG | 0.581083 | 4.1:-54434160 | MS.gene95742:CDS |
| GTTCTTCTGAAGCATCAAGA+GGG | 0.581323 | 4.1:+54435619 | None:intergenic |
| GTGGACCTTCCAACAAGGCA+GGG | 0.584213 | 4.1:+54436899 | None:intergenic |
| ATCTCTCGGTTTCGAAACGC+CGG | 0.585583 | 4.1:+54440757 | None:intergenic |
| CTGCAGCAAAGGATAAGCCT+TGG | 0.586086 | 4.1:-54434935 | MS.gene95742:CDS |
| GTAAGGTCCAAAGTTGAGGA+AGG | 0.587368 | 4.1:+54440947 | None:intergenic |
| TCAGAAATTTATTAACGCTG+TGG | 0.590172 | 4.1:-54434365 | MS.gene95742:CDS |
| GAACATGAAACAGGTTGACT+TGG | 0.593153 | 4.1:-54434248 | MS.gene95742:intron |
| ATTAGCAAAACTCAAAGGAG+TGG | 0.611195 | 4.1:+54436880 | None:intergenic |
| GTTGGATGAGGAGTTTGTCA+CGG | 0.615270 | 4.1:-54440789 | MS.gene95742:CDS |
| TTCTTCTGAAGCATCAAGAG+GGG | 0.617475 | 4.1:+54435620 | None:intergenic |
| GACATGGGTTAGTGGGGACA+AGG | 0.620413 | 4.1:-54436277 | MS.gene95742:CDS |
| GAGATTGACATGGGTTAGTG+GGG | 0.620979 | 4.1:-54436283 | MS.gene95742:CDS |
| GTAAACTCAGTGTTTCACAT+TGG | 0.623186 | 4.1:-54435267 | MS.gene95742:CDS |
| TATGTTACTCCTGACTCTGG+TGG | 0.623778 | 4.1:-54434999 | MS.gene95742:CDS |
| CCTTGTTCAATAGAGTACCA+AGG | 0.623949 | 4.1:+54434918 | None:intergenic |
| TGTTGCAGATGAGATTGACA+TGG | 0.630845 | 4.1:-54436293 | MS.gene95742:intron |
| TAAGGTCCAAAGTTGAGGAA+GGG | 0.632550 | 4.1:+54440948 | None:intergenic |
| TTCTCTCATAGTTATGCACA+TGG | 0.637196 | 4.1:+54434215 | None:intergenic |
| CCAATGTACACTTCCAACAA+CGG | 0.640358 | 4.1:-54434403 | MS.gene95742:CDS |
| TGTTCTTCTGAAGCATCAAG+AGG | 0.645423 | 4.1:+54435618 | None:intergenic |
| CAGCAATCGGGAATCATGAG+AGG | 0.646921 | 4.1:-54435155 | MS.gene95742:intron |
| AAGGAGTGGACCTTCCAACA+AGG | 0.647985 | 4.1:+54436894 | None:intergenic |
| TACCCCATGAAAATCAGCCA+AGG | 0.655808 | 4.1:+54434696 | None:intergenic |
| ACAGGGTAATGGCAAAGCAG+AGG | 0.665481 | 4.1:+54440534 | None:intergenic |
| CTTACTCCTACAGATATGGA+AGG | 0.668628 | 4.1:-54435972 | MS.gene95742:intron |
| TGCAGTGTGACAACTTGTAG+TGG | 0.671946 | 4.1:-54436995 | MS.gene95742:CDS |
| GTTGCAGATGAGATTGACAT+GGG | 0.725180 | 4.1:-54436292 | MS.gene95742:intron |
| GTGCATGACATACCTTGACA+GGG | 0.763616 | 4.1:+54440517 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 54433308 | 54440991 | 54433308 | ID=MS.gene95742 |
| chr4.1 | mRNA | 54433308 | 54440991 | 54433308 | ID=MS.gene95742.t1;Parent=MS.gene95742 |
| chr4.1 | exon | 54440719 | 54440991 | 54440719 | ID=MS.gene95742.t1.exon1;Parent=MS.gene95742.t1 |
| chr4.1 | CDS | 54440719 | 54440991 | 54440719 | ID=cds.MS.gene95742.t1;Parent=MS.gene95742.t1 |
| chr4.1 | exon | 54440530 | 54440617 | 54440530 | ID=MS.gene95742.t1.exon2;Parent=MS.gene95742.t1 |
| chr4.1 | CDS | 54440530 | 54440617 | 54440530 | ID=cds.MS.gene95742.t1;Parent=MS.gene95742.t1 |
| chr4.1 | exon | 54436826 | 54437092 | 54436826 | ID=MS.gene95742.t1.exon3;Parent=MS.gene95742.t1 |
| chr4.1 | CDS | 54436826 | 54437092 | 54436826 | ID=cds.MS.gene95742.t1;Parent=MS.gene95742.t1 |
| chr4.1 | exon | 54436199 | 54436307 | 54436199 | ID=MS.gene95742.t1.exon4;Parent=MS.gene95742.t1 |
| chr4.1 | CDS | 54436199 | 54436307 | 54436199 | ID=cds.MS.gene95742.t1;Parent=MS.gene95742.t1 |
| chr4.1 | exon | 54435973 | 54436087 | 54435973 | ID=MS.gene95742.t1.exon5;Parent=MS.gene95742.t1 |
| chr4.1 | CDS | 54435973 | 54436087 | 54435973 | ID=cds.MS.gene95742.t1;Parent=MS.gene95742.t1 |
| chr4.1 | exon | 54435608 | 54435740 | 54435608 | ID=MS.gene95742.t1.exon6;Parent=MS.gene95742.t1 |
| chr4.1 | CDS | 54435608 | 54435740 | 54435608 | ID=cds.MS.gene95742.t1;Parent=MS.gene95742.t1 |
| chr4.1 | exon | 54435156 | 54435343 | 54435156 | ID=MS.gene95742.t1.exon7;Parent=MS.gene95742.t1 |
| chr4.1 | CDS | 54435156 | 54435343 | 54435156 | ID=cds.MS.gene95742.t1;Parent=MS.gene95742.t1 |
| chr4.1 | exon | 54434860 | 54435046 | 54434860 | ID=MS.gene95742.t1.exon8;Parent=MS.gene95742.t1 |
| chr4.1 | CDS | 54434860 | 54435046 | 54434860 | ID=cds.MS.gene95742.t1;Parent=MS.gene95742.t1 |
| chr4.1 | exon | 54434699 | 54434766 | 54434699 | ID=MS.gene95742.t1.exon9;Parent=MS.gene95742.t1 |
| chr4.1 | CDS | 54434699 | 54434766 | 54434699 | ID=cds.MS.gene95742.t1;Parent=MS.gene95742.t1 |
| chr4.1 | exon | 54434342 | 54434432 | 54434342 | ID=MS.gene95742.t1.exon10;Parent=MS.gene95742.t1 |
| chr4.1 | CDS | 54434342 | 54434432 | 54434342 | ID=cds.MS.gene95742.t1;Parent=MS.gene95742.t1 |
| chr4.1 | exon | 54434069 | 54434257 | 54434069 | ID=MS.gene95742.t1.exon11;Parent=MS.gene95742.t1 |
| chr4.1 | CDS | 54434069 | 54434257 | 54434069 | ID=cds.MS.gene95742.t1;Parent=MS.gene95742.t1 |
| chr4.1 | exon | 54433484 | 54433564 | 54433484 | ID=MS.gene95742.t1.exon12;Parent=MS.gene95742.t1 |
| chr4.1 | CDS | 54433484 | 54433564 | 54433484 | ID=cds.MS.gene95742.t1;Parent=MS.gene95742.t1 |
| chr4.1 | exon | 54433308 | 54433361 | 54433308 | ID=MS.gene95742.t1.exon13;Parent=MS.gene95742.t1 |
| chr4.1 | CDS | 54433308 | 54433361 | 54433308 | ID=cds.MS.gene95742.t1;Parent=MS.gene95742.t1 |
| Gene Sequence |
| Protein sequence |
