Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96329.t1 | XP_003610026.1 | 89.3 | 103 | 1 | 1 | 1 | 93 | 502 | 604 | 3.10E-37 | 164.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96329.t1 | Q651V6 | 82.4 | 102 | 8 | 1 | 1 | 92 | 507 | 608 | 5.1e-36 | 151.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96329.t1 | Q84YG8 | 89.3 | 103 | 1 | 1 | 1 | 93 | 502 | 604 | 2.3e-37 | 164.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051832 | MS.gene96329 | 0.810278 | 1.25E-50 | -1.69E-46 |
| MS.gene05365 | MS.gene96329 | 0.812192 | 4.80E-51 | -1.69E-46 |
| MS.gene057102 | MS.gene96329 | 0.806097 | 9.70E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96329.t1 | MTR_4g127100 | 89.320 | 103 | 1 | 1 | 1 | 93 | 502 | 604 | 1.92e-53 | 175 |
| MS.gene96329.t1 | MTR_4g127090 | 74.757 | 103 | 16 | 1 | 1 | 93 | 444 | 546 | 1.46e-44 | 151 |
| MS.gene96329.t1 | MTR_8g107360 | 72.816 | 103 | 18 | 1 | 1 | 93 | 401 | 503 | 1.78e-43 | 147 |
| MS.gene96329.t1 | MTR_1g030890 | 73.529 | 102 | 17 | 1 | 1 | 92 | 498 | 599 | 4.70e-43 | 147 |
| MS.gene96329.t1 | MTR_4g084870 | 73.786 | 103 | 17 | 1 | 1 | 93 | 536 | 638 | 1.87e-42 | 146 |
| MS.gene96329.t1 | MTR_7g089360 | 71.845 | 103 | 19 | 1 | 1 | 93 | 468 | 570 | 2.02e-42 | 145 |
| MS.gene96329.t1 | MTR_7g089360 | 71.845 | 103 | 19 | 1 | 1 | 93 | 468 | 570 | 2.08e-42 | 145 |
| MS.gene96329.t1 | MTR_6g069510 | 70.874 | 103 | 20 | 1 | 1 | 93 | 481 | 583 | 5.26e-42 | 144 |
| MS.gene96329.t1 | MTR_1g029190 | 65.049 | 103 | 26 | 1 | 1 | 93 | 404 | 506 | 6.52e-38 | 132 |
| MS.gene96329.t1 | MTR_2g038470 | 60.784 | 102 | 30 | 1 | 1 | 92 | 386 | 487 | 1.13e-33 | 121 |
| MS.gene96329.t1 | MTR_6g011400 | 58.252 | 103 | 33 | 1 | 1 | 93 | 238 | 340 | 2.18e-33 | 118 |
| MS.gene96329.t1 | MTR_7g009370 | 56.311 | 103 | 35 | 1 | 1 | 93 | 234 | 336 | 7.34e-32 | 114 |
| MS.gene96329.t1 | MTR_7g079720 | 44.660 | 103 | 47 | 1 | 1 | 93 | 238 | 340 | 6.59e-25 | 95.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96329.t1 | AT5G57090 | 79.412 | 102 | 11 | 1 | 1 | 92 | 424 | 525 | 2.26e-46 | 155 |
| MS.gene96329.t1 | AT5G57090 | 79.412 | 102 | 11 | 1 | 1 | 92 | 524 | 625 | 6.69e-46 | 155 |
| MS.gene96329.t1 | AT2G01420 | 69.903 | 103 | 21 | 1 | 1 | 93 | 493 | 595 | 2.57e-40 | 140 |
| MS.gene96329.t1 | AT2G01420 | 69.903 | 103 | 21 | 1 | 1 | 93 | 489 | 591 | 2.85e-40 | 140 |
| MS.gene96329.t1 | AT1G70940 | 73.786 | 103 | 17 | 1 | 1 | 93 | 517 | 619 | 2.66e-38 | 134 |
| MS.gene96329.t1 | AT1G23080 | 70.874 | 103 | 20 | 1 | 1 | 93 | 492 | 594 | 7.33e-38 | 133 |
| MS.gene96329.t1 | AT1G23080 | 70.874 | 103 | 20 | 1 | 1 | 93 | 492 | 594 | 7.33e-38 | 133 |
| MS.gene96329.t1 | AT1G23080 | 70.874 | 103 | 20 | 1 | 1 | 93 | 496 | 598 | 7.42e-38 | 133 |
| MS.gene96329.t1 | AT1G77110 | 60.784 | 102 | 30 | 1 | 1 | 92 | 447 | 548 | 1.47e-34 | 124 |
| MS.gene96329.t1 | AT1G77110 | 60.784 | 102 | 30 | 1 | 1 | 92 | 447 | 548 | 1.56e-34 | 124 |
| MS.gene96329.t1 | AT1G73590 | 66.667 | 102 | 24 | 1 | 1 | 92 | 499 | 600 | 1.09e-32 | 119 |
| MS.gene96329.t1 | AT5G15100 | 52.427 | 103 | 39 | 1 | 1 | 93 | 244 | 346 | 5.73e-31 | 112 |
| MS.gene96329.t1 | AT5G16530 | 47.059 | 102 | 44 | 1 | 1 | 92 | 226 | 327 | 3.32e-24 | 93.6 |
| MS.gene96329.t1 | AT5G16530 | 46.602 | 103 | 45 | 1 | 1 | 93 | 226 | 328 | 4.63e-24 | 93.6 |
Find 27 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACCCAGTGCTGAGTATATT+TGG | 0.231032 | 8.4:+7762795 | None:intergenic |
| GAAATGCCATCTATTGTAAA+AGG | 0.235112 | 8.4:-7763291 | MS.gene96329:CDS |
| GTTACATGTTGCAATTGTTC+AGG | 0.321229 | 8.4:-7762980 | MS.gene96329:intron |
| TGGATGGAGATTGTATTCTT+TGG | 0.334269 | 8.4:+7762815 | None:intergenic |
| ATACTGTCTGATGCTGGTCT+TGG | 0.436774 | 8.4:-7763258 | MS.gene96329:CDS |
| ACGGATACCAATTGCTATTG+AGG | 0.461284 | 8.4:+7763011 | None:intergenic |
| CACAAAGGGAACGATACCTT+GGG | 0.473023 | 8.4:+7762842 | None:intergenic |
| ATCTCAATACTGTCTGATGC+TGG | 0.484824 | 8.4:-7763264 | MS.gene96329:CDS |
| GCGGCAACCTCAATAGCAAT+TGG | 0.493077 | 8.4:-7763018 | MS.gene96329:CDS |
| TATTCTTTGGCAAACACAAA+GGG | 0.501329 | 8.4:+7762828 | None:intergenic |
| ACACAAAGGGAACGATACCT+TGG | 0.508083 | 8.4:+7762841 | None:intergenic |
| CATCCAAATATACTCAGCAC+TGG | 0.523771 | 8.4:-7762798 | MS.gene96329:CDS |
| TGCCGCAATCACAGTTGGAC+CGG | 0.524372 | 8.4:+7763035 | None:intergenic |
| GTCTGATGCTGGTCTTGGAA+TGG | 0.532632 | 8.4:-7763253 | MS.gene96329:CDS |
| ATGGCTGTTAGGTTCTTGAC+CGG | 0.534223 | 8.4:-7763054 | MS.gene96329:CDS |
| GAGGTTGCCGCAATCACAGT+TGG | 0.546063 | 8.4:+7763030 | None:intergenic |
| GACCGGTCCAACTGTGATTG+CGG | 0.556573 | 8.4:-7763037 | MS.gene96329:CDS |
| TCAATAGCAATTGGTATCCG+TGG | 0.585503 | 8.4:-7763009 | MS.gene96329:CDS |
| AGCATCAGACAGTATTGAGA+TGG | 0.585685 | 8.4:+7763266 | None:intergenic |
| CAGTGCTGAGTATATTTGGA+TGG | 0.585744 | 8.4:+7762799 | None:intergenic |
| CAACCTAAGCTGATTGCATG+TGG | 0.587393 | 8.4:-7763099 | MS.gene96329:intron |
| GTATTCTTTGGCAAACACAA+AGG | 0.589188 | 8.4:+7762827 | None:intergenic |
| TTGCAGGCTGCACTTCCCCA+AGG | 0.623512 | 8.4:-7762858 | MS.gene96329:intron |
| ATCCAAATATACTCAGCACT+GGG | 0.630722 | 8.4:-7762797 | MS.gene96329:CDS |
| ATAAGTACTTACACTGAACA+TGG | 0.642188 | 8.4:+7763230 | None:intergenic |
| CAACATGTAACAGAACTCCA+CGG | 0.711215 | 8.4:+7762992 | None:intergenic |
| ACAAAGGGAACGATACCTTG+GGG | 0.759468 | 8.4:+7762843 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAACAACTAATTAGAATA+AGG | + | chr8.4:7763179-7763198 | None:intergenic | 15.0% |
| !!! | AATGTTTAATTTTAATTTGC+AGG | - | chr8.4:7763208-7763227 | MS.gene96329:intron | 15.0% |
| !!! | TTTATTATGAATTTTTATGC+AGG | - | chr8.4:7762944-7762963 | MS.gene96329:intron | 15.0% |
| !!! | AATTAAGCCAAAATTTTCTT+AGG | + | chr8.4:7762899-7762918 | None:intergenic | 20.0% |
| !!! | ATATTCTCCTAAGAAAATTT+TGG | - | chr8.4:7762889-7762908 | MS.gene96329:intron | 20.0% |
| ! | TCTTAGGAGAATATAATGTT+TGG | + | chr8.4:7762883-7762902 | None:intergenic | 25.0% |
| ATAAGTACTTACACTGAACA+TGG | + | chr8.4:7762855-7762874 | None:intergenic | 30.0% | |
| TATTCTTTGGCAAACACAAA+GGG | + | chr8.4:7763257-7763276 | None:intergenic | 30.0% | |
| ! | ATGGAACCTTTTACAATAGA+TGG | + | chr8.4:7762800-7762819 | None:intergenic | 30.0% |
| ATCCAAATATACTCAGCACT+GGG | - | chr8.4:7763285-7763304 | MS.gene96329:CDS | 35.0% | |
| GTATTCTTTGGCAAACACAA+AGG | + | chr8.4:7763258-7763277 | None:intergenic | 35.0% | |
| GTTACATGTTGCAATTGTTC+AGG | - | chr8.4:7763102-7763121 | MS.gene96329:intron | 35.0% | |
| TGGATGGAGATTGTATTCTT+TGG | + | chr8.4:7763270-7763289 | None:intergenic | 35.0% | |
| ! | TTTTTATGCAGGTCTGTTCA+TGG | - | chr8.4:7762955-7762974 | MS.gene96329:intron | 35.0% |
| ATCTCAATACTGTCTGATGC+TGG | - | chr8.4:7762818-7762837 | MS.gene96329:CDS | 40.0% | |
| CAACATGTAACAGAACTCCA+CGG | + | chr8.4:7763093-7763112 | None:intergenic | 40.0% | |
| CATCCAAATATACTCAGCAC+TGG | - | chr8.4:7763284-7763303 | MS.gene96329:CDS | 40.0% | |
| TCAATAGCAATTGGTATCCG+TGG | - | chr8.4:7763073-7763092 | MS.gene96329:CDS | 40.0% | |
| TTTCCACATGCAATCAGCTT+AGG | + | chr8.4:7762989-7763008 | None:intergenic | 40.0% | |
| ! | ACGAGTTGCAACGTTTTCTA+TGG | - | chr8.4:7763009-7763028 | MS.gene96329:CDS | 40.0% |
| ! | ACGGATACCAATTGCTATTG+AGG | + | chr8.4:7763074-7763093 | None:intergenic | 40.0% |
| ! | AGCATCAGACAGTATTGAGA+TGG | + | chr8.4:7762819-7762838 | None:intergenic | 40.0% |
| ! | CAACGTTTTCTATGGCTGTT+AGG | - | chr8.4:7763017-7763036 | MS.gene96329:CDS | 40.0% |
| ! | CAGTGCTGAGTATATTTGGA+TGG | + | chr8.4:7763286-7763305 | None:intergenic | 40.0% |
| ACAAAGGGAACGATACCTTG+GGG | + | chr8.4:7763242-7763261 | None:intergenic | 45.0% | |
| ACACAAAGGGAACGATACCT+TGG | + | chr8.4:7763244-7763263 | None:intergenic | 45.0% | |
| ATGGCTGTTAGGTTCTTGAC+CGG | - | chr8.4:7763028-7763047 | MS.gene96329:CDS | 45.0% | |
| CAACCTAAGCTGATTGCATG+TGG | - | chr8.4:7762983-7763002 | MS.gene96329:CDS | 45.0% | |
| CACAAAGGGAACGATACCTT+GGG | + | chr8.4:7763243-7763262 | None:intergenic | 45.0% | |
| ! | ATACTGTCTGATGCTGGTCT+TGG | - | chr8.4:7762824-7762843 | MS.gene96329:CDS | 45.0% |
| ! | CACCCAGTGCTGAGTATATT+TGG | + | chr8.4:7763290-7763309 | None:intergenic | 45.0% |
| GCGGCAACCTCAATAGCAAT+TGG | - | chr8.4:7763064-7763083 | MS.gene96329:CDS | 50.0% | |
| !! | GTCTGATGCTGGTCTTGGAA+TGG | - | chr8.4:7762829-7762848 | MS.gene96329:CDS | 50.0% |
| GACCGGTCCAACTGTGATTG+CGG | - | chr8.4:7763045-7763064 | MS.gene96329:CDS | 55.0% | |
| GAGGTTGCCGCAATCACAGT+TGG | + | chr8.4:7763055-7763074 | None:intergenic | 55.0% | |
| TGCCGCAATCACAGTTGGAC+CGG | + | chr8.4:7763050-7763069 | None:intergenic | 55.0% | |
| TTGCAGGCTGCACTTCCCCA+AGG | - | chr8.4:7763224-7763243 | MS.gene96329:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 7762794 | 7763310 | 7762794 | ID=MS.gene96329 |
| chr8.4 | mRNA | 7762794 | 7763310 | 7762794 | ID=MS.gene96329.t1;Parent=MS.gene96329 |
| chr8.4 | exon | 7763242 | 7763310 | 7763242 | ID=MS.gene96329.t1.exon1;Parent=MS.gene96329.t1 |
| chr8.4 | CDS | 7763242 | 7763310 | 7763242 | ID=cds.MS.gene96329.t1;Parent=MS.gene96329.t1 |
| chr8.4 | exon | 7762981 | 7763112 | 7762981 | ID=MS.gene96329.t1.exon2;Parent=MS.gene96329.t1 |
| chr8.4 | CDS | 7762981 | 7763112 | 7762981 | ID=cds.MS.gene96329.t1;Parent=MS.gene96329.t1 |
| chr8.4 | exon | 7762794 | 7762874 | 7762794 | ID=MS.gene96329.t1.exon3;Parent=MS.gene96329.t1 |
| chr8.4 | CDS | 7762794 | 7762874 | 7762794 | ID=cds.MS.gene96329.t1;Parent=MS.gene96329.t1 |
| Gene Sequence |
| Protein sequence |
