AlfalfaGEDB | MS.gene05365

M. sativa cultivar XinJiangDaYe / MS.gene05365

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05365.t1	XP_003620416.1	91.6	107	8	1	9	114	73	179	4.30E-20	107.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05365.t1	G7KMM1	91.6	107	8	1	9	114	73	179	3.1e-20	107.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049666	MS.gene05365	0.829176	5.96E-55	-1.69E-46
MS.gene049825	MS.gene05365	0.823516	1.33E-53	-1.69E-46
MS.gene049873	MS.gene05365	0.804161	2.47E-49	-1.69E-46
MS.gene050026	MS.gene05365	0.808969	2.39E-50	-1.69E-46
MS.gene050355	MS.gene05365	0.830695	2.54E-55	-1.69E-46
MS.gene051594	MS.gene05365	0.818142	2.29E-52	-1.69E-46
MS.gene051957	MS.gene05365	0.804093	2.55E-49	-1.69E-46
MS.gene052519	MS.gene05365	0.804222	2.40E-49	-1.69E-46
MS.gene052582	MS.gene05365	0.83106	2.07E-55	-1.69E-46
MS.gene052584	MS.gene05365	0.841549	4.44E-58	-1.69E-46
MS.gene052685	MS.gene05365	0.831172	1.94E-55	-1.69E-46
MS.gene053006	MS.gene05365	0.806578	7.69E-50	-1.69E-46
MS.gene053226	MS.gene05365	0.819065	1.41E-52	-1.69E-46
MS.gene05365	MS.gene055586	0.81141	7.10E-51	-1.69E-46
MS.gene05365	MS.gene055778	0.844341	8.03E-59	-1.69E-46
MS.gene05365	MS.gene056091	0.822942	1.81E-53	-1.69E-46
MS.gene05365	MS.gene056174	0.812971	3.24E-51	-1.69E-46
MS.gene05365	MS.gene05689	0.82011	8.15E-53	-1.69E-46
MS.gene05365	MS.gene057314	0.808671	2.76E-50	-1.69E-46
MS.gene05365	MS.gene058037	0.839141	1.89E-57	-1.69E-46
MS.gene05365	MS.gene05953	0.807628	4.61E-50	-1.69E-46
MS.gene05365	MS.gene05954	0.842268	2.87E-58	-1.69E-46
MS.gene05365	MS.gene059625	0.829485	5.01E-55	-1.69E-46
MS.gene05365	MS.gene059935	0.835436	1.68E-56	-1.69E-46
MS.gene05365	MS.gene060521	0.833347	5.62E-56	-1.69E-46
MS.gene05365	MS.gene060879	0.803718	3.05E-49	-1.69E-46
MS.gene05365	MS.gene061040	-0.803166	3.97E-49	-1.69E-46
MS.gene05365	MS.gene061594	0.845653	3.55E-59	-1.69E-46
MS.gene05365	MS.gene062672	0.802929	4.44E-49	-1.69E-46
MS.gene05365	MS.gene06269	0.824418	8.16E-54	-1.69E-46
MS.gene05365	MS.gene064321	0.842075	3.23E-58	-1.69E-46
MS.gene05365	MS.gene065125	0.815328	9.78E-52	-1.69E-46
MS.gene05365	MS.gene065446	0.81059	1.07E-50	-1.69E-46
MS.gene05365	MS.gene065737	0.808743	2.67E-50	-1.69E-46
MS.gene05365	MS.gene065993	0.822894	1.86E-53	-1.69E-46
MS.gene05365	MS.gene066267	0.808663	2.77E-50	-1.69E-46
MS.gene05365	MS.gene067208	0.815028	1.14E-51	-1.69E-46
MS.gene05365	MS.gene068159	0.812512	4.08E-51	-1.69E-46
MS.gene05365	MS.gene068350	0.824554	7.58E-54	-1.69E-46
MS.gene05365	MS.gene068385	0.802398	5.72E-49	-1.69E-46
MS.gene05365	MS.gene068456	0.805715	1.17E-49	-1.69E-46
MS.gene05365	MS.gene068924	0.813034	3.14E-51	-1.69E-46
MS.gene05365	MS.gene069024	0.802218	6.23E-49	-1.69E-46
MS.gene05365	MS.gene070301	0.81852	1.88E-52	-1.69E-46
MS.gene05365	MS.gene070627	0.835692	1.45E-56	-1.69E-46
MS.gene05365	MS.gene070818	0.806917	6.52E-50	-1.69E-46
MS.gene05365	MS.gene071034	0.80816	3.55E-50	-1.69E-46
MS.gene05365	MS.gene071322	0.83386	4.18E-56	-1.69E-46
MS.gene05365	MS.gene071342	0.810101	1.36E-50	-1.69E-46
MS.gene05365	MS.gene071442	0.811269	7.62E-51	-1.69E-46
MS.gene05365	MS.gene071444	0.809244	2.08E-50	-1.69E-46
MS.gene05365	MS.gene071568	0.814584	1.43E-51	-1.69E-46
MS.gene05365	MS.gene071570	0.814539	1.46E-51	-1.69E-46
MS.gene05365	MS.gene071987	0.82957	4.78E-55	-1.69E-46
MS.gene05365	MS.gene072067	0.81014	1.34E-50	-1.69E-46
MS.gene05365	MS.gene072280	0.870499	1.39E-66	-1.69E-46
MS.gene05365	MS.gene072299	0.869053	4.13E-66	-1.69E-46
MS.gene05365	MS.gene072678	0.818388	2.01E-52	-1.69E-46
MS.gene05365	MS.gene072955	0.809487	1.85E-50	-1.69E-46
MS.gene05365	MS.gene073053	0.804293	2.32E-49	-1.69E-46
MS.gene05365	MS.gene073300	0.810106	1.36E-50	-1.69E-46
MS.gene05365	MS.gene073301	0.830609	2.67E-55	-1.69E-46
MS.gene05365	MS.gene073665	0.804821	1.80E-49	-1.69E-46
MS.gene05365	MS.gene07376	0.801825	7.50E-49	-1.69E-46
MS.gene05365	MS.gene07394	0.83258	8.72E-56	-1.69E-46
MS.gene05365	MS.gene074356	0.803216	3.87E-49	-1.69E-46
MS.gene05365	MS.gene07529	0.807392	5.17E-50	-1.69E-46
MS.gene05365	MS.gene07719	0.812678	3.76E-51	-1.69E-46
MS.gene05365	MS.gene07815	0.816175	6.33E-52	-1.69E-46
MS.gene05365	MS.gene07876	0.801873	7.33E-49	-1.69E-46
MS.gene05365	MS.gene08554	0.802188	6.32E-49	-1.69E-46
MS.gene05365	MS.gene08618	0.825446	4.67E-54	-1.69E-46
MS.gene05365	MS.gene08824	0.814494	1.50E-51	-1.69E-46
MS.gene05365	MS.gene08862	0.8515	8.53E-61	-1.69E-46
MS.gene05365	MS.gene09233	0.841697	4.06E-58	-1.69E-46
MS.gene05365	MS.gene20655	0.811778	5.91E-51	-1.69E-46
MS.gene05365	MS.gene21700	0.820655	6.11E-53	-1.69E-46
MS.gene05365	MS.gene21926	0.829028	6.47E-55	-1.69E-46
MS.gene05365	MS.gene21928	0.837245	5.82E-57	-1.69E-46
MS.gene05365	MS.gene22255	0.849181	3.81E-60	-1.69E-46
MS.gene05365	MS.gene22270	0.840261	9.67E-58	-1.69E-46
MS.gene05365	MS.gene22976	0.819202	1.31E-52	-1.69E-46
MS.gene05365	MS.gene23697	0.812826	3.49E-51	-1.69E-46
MS.gene05365	MS.gene24623	0.831548	1.57E-55	-1.69E-46
MS.gene05365	MS.gene25207	0.831854	1.32E-55	-1.69E-46
MS.gene05365	MS.gene25349	0.813305	2.74E-51	-1.69E-46
MS.gene05365	MS.gene25350	0.802807	4.71E-49	-1.69E-46
MS.gene05365	MS.gene25991	0.822222	2.66E-53	-1.69E-46
MS.gene05365	MS.gene26062	0.823424	1.40E-53	-1.69E-46
MS.gene05365	MS.gene27041	0.801802	7.58E-49	-1.69E-46
MS.gene05365	MS.gene27516	0.852147	5.59E-61	-1.69E-46
MS.gene05365	MS.gene28652	0.834197	3.44E-56	-1.69E-46
MS.gene05365	MS.gene28657	0.807421	5.10E-50	-1.69E-46
MS.gene05365	MS.gene30085	0.80971	1.65E-50	-1.69E-46
MS.gene05365	MS.gene30970	0.80622	9.14E-50	-1.69E-46
MS.gene05365	MS.gene31142	0.81744	3.29E-52	-1.69E-46
MS.gene05365	MS.gene31468	0.801466	8.89E-49	-1.69E-46
MS.gene05365	MS.gene31658	0.809985	1.44E-50	-1.69E-46
MS.gene05365	MS.gene32229	0.807751	4.34E-50	-1.69E-46
MS.gene05365	MS.gene33129	0.81255	4.01E-51	-1.69E-46
MS.gene05365	MS.gene33131	0.826867	2.14E-54	-1.69E-46
MS.gene05365	MS.gene33650	0.840065	1.09E-57	-1.69E-46
MS.gene05365	MS.gene33651	0.802337	5.89E-49	-1.69E-46
MS.gene05365	MS.gene33853	0.84009	1.07E-57	-1.69E-46
MS.gene05365	MS.gene34803	0.812473	4.16E-51	-1.69E-46
MS.gene05365	MS.gene35491	0.801885	7.29E-49	-1.69E-46
MS.gene05365	MS.gene36229	0.808498	3.01E-50	-1.69E-46
MS.gene05365	MS.gene36231	0.803719	3.05E-49	-1.69E-46
MS.gene05365	MS.gene36241	0.830428	2.95E-55	-1.69E-46
MS.gene05365	MS.gene36472	0.808996	2.35E-50	-1.69E-46
MS.gene05365	MS.gene36492	0.80254	5.35E-49	-1.69E-46
MS.gene05365	MS.gene36987	0.811793	5.86E-51	-1.69E-46
MS.gene05365	MS.gene37027	0.833919	4.04E-56	-1.69E-46
MS.gene05365	MS.gene37029	0.844672	6.54E-59	-1.69E-46
MS.gene05365	MS.gene37612	0.822502	2.29E-53	-1.69E-46
MS.gene05365	MS.gene38659	0.809263	2.06E-50	-1.69E-46
MS.gene05365	MS.gene39207	0.816344	5.80E-52	-1.69E-46
MS.gene05365	MS.gene40022	0.834278	3.29E-56	-1.69E-46
MS.gene05365	MS.gene40195	0.809962	1.46E-50	-1.69E-46
MS.gene05365	MS.gene40259	0.809628	1.72E-50	-1.69E-46
MS.gene05365	MS.gene40348	0.80928	2.05E-50	-1.69E-46
MS.gene05365	MS.gene40709	0.848171	7.26E-60	-1.69E-46
MS.gene05365	MS.gene40711	0.833805	4.32E-56	-1.69E-46
MS.gene05365	MS.gene43372	0.822381	2.44E-53	-1.69E-46
MS.gene05365	MS.gene43946	0.814012	1.91E-51	-1.69E-46
MS.gene05365	MS.gene43970	0.807587	4.70E-50	-1.69E-46
MS.gene05365	MS.gene44077	0.803053	4.19E-49	-1.69E-46
MS.gene05365	MS.gene44256	0.812206	4.76E-51	-1.69E-46
MS.gene05365	MS.gene44986	0.805069	1.59E-49	-1.69E-46
MS.gene05365	MS.gene45507	0.810143	1.33E-50	-1.69E-46
MS.gene05365	MS.gene45524	0.801074	1.07E-48	-1.69E-46
MS.gene05365	MS.gene46997	0.808468	3.05E-50	-1.69E-46
MS.gene05365	MS.gene48323	0.81334	2.69E-51	-1.69E-46
MS.gene05365	MS.gene48368	0.802411	5.68E-49	-1.69E-46
MS.gene05365	MS.gene48389	0.834362	3.13E-56	-1.69E-46
MS.gene05365	MS.gene49386	0.820977	5.16E-53	-1.69E-46
MS.gene05365	MS.gene50493	0.825149	5.49E-54	-1.69E-46
MS.gene05365	MS.gene50546	0.807927	3.98E-50	-1.69E-46
MS.gene05365	MS.gene50615	0.810334	1.21E-50	-1.69E-46
MS.gene05365	MS.gene50875	0.809391	1.94E-50	-1.69E-46
MS.gene05365	MS.gene50986	0.801625	8.24E-49	-1.69E-46
MS.gene05365	MS.gene52130	0.813982	1.94E-51	-1.69E-46
MS.gene05365	MS.gene53175	0.802043	6.77E-49	-1.69E-46
MS.gene05365	MS.gene54197	0.811495	6.80E-51	-1.69E-46
MS.gene05365	MS.gene54368	0.805473	1.31E-49	-1.69E-46
MS.gene05365	MS.gene54538	0.806092	9.73E-50	-1.69E-46
MS.gene05365	MS.gene54656	0.811601	6.45E-51	-1.69E-46
MS.gene05365	MS.gene55019	0.805336	1.40E-49	-1.69E-46
MS.gene05365	MS.gene55022	0.805376	1.38E-49	-1.69E-46
MS.gene05365	MS.gene55023	0.801481	8.82E-49	-1.69E-46
MS.gene05365	MS.gene55381	0.828716	7.70E-55	-1.69E-46
MS.gene05365	MS.gene55651	0.808648	2.79E-50	-1.69E-46
MS.gene05365	MS.gene55818	0.809984	1.44E-50	-1.69E-46
MS.gene05365	MS.gene56468	0.820712	5.93E-53	-1.69E-46
MS.gene05365	MS.gene56924	0.818128	2.30E-52	-1.69E-46
MS.gene05365	MS.gene57498	0.815925	7.20E-52	-1.69E-46
MS.gene05365	MS.gene58719	0.803883	2.82E-49	-1.69E-46
MS.gene05365	MS.gene59540	0.833634	4.76E-56	-1.69E-46
MS.gene05365	MS.gene59541	0.806215	9.17E-50	-1.69E-46
MS.gene05365	MS.gene60357	0.821892	3.17E-53	-1.69E-46
MS.gene05365	MS.gene61236	0.81086	9.34E-51	-1.69E-46
MS.gene05365	MS.gene61288	0.823756	1.17E-53	-1.69E-46
MS.gene05365	MS.gene63809	0.803964	2.71E-49	-1.69E-46
MS.gene05365	MS.gene63811	0.812727	3.66E-51	-1.69E-46
MS.gene05365	MS.gene63988	0.828171	1.04E-54	-1.69E-46
MS.gene05365	MS.gene66077	0.806043	9.96E-50	-1.69E-46
MS.gene05365	MS.gene66339	0.815131	1.08E-51	-1.69E-46
MS.gene05365	MS.gene66474	0.80248	5.50E-49	-1.69E-46
MS.gene05365	MS.gene68198	0.819128	1.37E-52	-1.69E-46
MS.gene05365	MS.gene68390	0.81899	1.47E-52	-1.69E-46
MS.gene05365	MS.gene68824	0.831557	1.56E-55	-1.69E-46
MS.gene05365	MS.gene68826	0.816904	4.35E-52	-1.69E-46
MS.gene05365	MS.gene68904	0.804225	2.39E-49	-1.69E-46
MS.gene05365	MS.gene68999	0.827252	1.73E-54	-1.69E-46
MS.gene05365	MS.gene69214	0.835036	2.12E-56	-1.69E-46
MS.gene05365	MS.gene69780	0.800123	1.67E-48	-1.69E-46
MS.gene05365	MS.gene70656	0.837569	4.81E-57	-1.69E-46
MS.gene05365	MS.gene71613	0.817073	3.98E-52	-1.69E-46
MS.gene05365	MS.gene71735	0.804351	2.25E-49	-1.69E-46
MS.gene05365	MS.gene71825	0.818278	2.13E-52	-1.69E-46
MS.gene05365	MS.gene71826	0.819992	8.68E-53	-1.69E-46
MS.gene05365	MS.gene71840	0.81662	5.03E-52	-1.69E-46
MS.gene05365	MS.gene71988	0.812602	3.90E-51	-1.69E-46
MS.gene05365	MS.gene72026	0.854375	1.28E-61	-1.69E-46
MS.gene05365	MS.gene72990	0.802073	6.67E-49	-1.69E-46
MS.gene05365	MS.gene73467	0.801174	1.02E-48	-1.69E-46
MS.gene05365	MS.gene73831	0.809096	2.24E-50	-1.69E-46
MS.gene05365	MS.gene74076	0.805162	1.52E-49	-1.69E-46
MS.gene05365	MS.gene74257	0.82058	6.36E-53	-1.69E-46
MS.gene05365	MS.gene74503	0.803997	2.67E-49	-1.69E-46
MS.gene05365	MS.gene74530	0.818713	1.70E-52	-1.69E-46
MS.gene05365	MS.gene74869	0.823513	1.33E-53	-1.69E-46
MS.gene05365	MS.gene75003	0.822345	2.49E-53	-1.69E-46
MS.gene05365	MS.gene75187	0.821839	3.26E-53	-1.69E-46
MS.gene05365	MS.gene75963	0.820179	7.86E-53	-1.69E-46
MS.gene05365	MS.gene76148	0.805044	1.61E-49	-1.69E-46
MS.gene05365	MS.gene76158	0.803505	3.38E-49	-1.69E-46
MS.gene05365	MS.gene76244	0.809648	1.71E-50	-1.69E-46
MS.gene05365	MS.gene77588	0.824362	8.41E-54	-1.69E-46
MS.gene05365	MS.gene79127	0.807776	4.28E-50	-1.69E-46
MS.gene05365	MS.gene79253	0.814003	1.92E-51	-1.69E-46
MS.gene05365	MS.gene79559	0.825595	4.30E-54	-1.69E-46
MS.gene05365	MS.gene80741	0.802888	4.53E-49	-1.69E-46
MS.gene05365	MS.gene81047	0.81813	2.30E-52	-1.69E-46
MS.gene05365	MS.gene81107	-0.811357	7.29E-51	-1.69E-46
MS.gene05365	MS.gene82311	0.838277	3.17E-57	-1.69E-46
MS.gene05365	MS.gene82387	0.811319	7.43E-51	-1.69E-46
MS.gene05365	MS.gene83091	0.831403	1.70E-55	-1.69E-46
MS.gene05365	MS.gene84732	0.8307	2.53E-55	-1.69E-46
MS.gene05365	MS.gene84803	0.823321	1.48E-53	-1.69E-46
MS.gene05365	MS.gene84865	0.825713	4.03E-54	-1.69E-46
MS.gene05365	MS.gene85641	0.800285	1.55E-48	-1.69E-46
MS.gene05365	MS.gene85978	0.811214	7.83E-51	-1.69E-46
MS.gene05365	MS.gene87285	0.807366	5.23E-50	-1.69E-46
MS.gene05365	MS.gene87377	0.80742	5.10E-50	-1.69E-46
MS.gene05365	MS.gene87673	0.836464	9.22E-57	-1.69E-46
MS.gene05365	MS.gene89277	0.805245	1.47E-49	-1.69E-46
MS.gene05365	MS.gene90651	0.818467	1.93E-52	-1.69E-46
MS.gene05365	MS.gene90926	0.800207	1.61E-48	-1.69E-46
MS.gene05365	MS.gene91004	0.801964	7.02E-49	-1.69E-46
MS.gene05365	MS.gene91204	0.809377	1.95E-50	-1.69E-46
MS.gene05365	MS.gene91310	0.842512	2.47E-58	-1.69E-46
MS.gene05365	MS.gene91676	0.805304	1.42E-49	-1.69E-46
MS.gene05365	MS.gene92059	0.847755	9.46E-60	-1.69E-46
MS.gene05365	MS.gene92775	0.827447	1.56E-54	-1.69E-46
MS.gene05365	MS.gene93501	0.809176	2.15E-50	-1.69E-46
MS.gene05365	MS.gene93690	0.80727	5.49E-50	-1.69E-46
MS.gene05365	MS.gene94906	0.805681	1.19E-49	-1.69E-46
MS.gene05365	MS.gene95655	0.817696	2.88E-52	-1.69E-46
MS.gene05365	MS.gene96329	0.812192	4.80E-51	-1.69E-46
MS.gene05365	MS.gene96338	0.808861	2.52E-50	-1.69E-46
MS.gene05365	MS.gene96693	0.822086	2.86E-53	-1.69E-46
MS.gene05365	MS.gene97439	0.806111	9.64E-50	-1.69E-46
MS.gene05365	MS.gene97609	0.818232	2.18E-52	-1.69E-46
MS.gene05365	MS.gene97766	0.825331	4.97E-54	-1.69E-46
MS.gene05365	MS.gene98461	0.803769	2.98E-49	-1.69E-46
MS.gene05365	MS.gene98953	0.801233	9.92E-49	-1.69E-46
MS.gene05365	MS.gene99849	0.825817	3.81E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05365.t1	MTR_6g082590	87.736	106	12	1	10	114	74	179	2.17e-47	150

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 29 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCCTGATCAGCATCTAT+TGG	0.420664	6.3:-75766487	None:intergenic
GATTTGATGAGGGATATTCT+AGG	0.428796	6.3:+75766505	MS.gene05365:CDS
AGACGAGTTTGATTCTGATT+CGG	0.441246	6.3:+75766603	MS.gene05365:CDS
GAAGCCAATAGATGCTGATC+AGG	0.453231	6.3:+75766483	MS.gene05365:CDS
TGCAATTCCAGAAGTCTTTG+AGG	0.475249	6.3:+75766435	MS.gene05365:CDS
TTTGAATACTAGTCTGAATG+AGG	0.476699	6.3:-75766123	None:intergenic
AATACTGCCTCAAAGACTTC+TGG	0.481399	6.3:-75766442	None:intergenic
GGTAGGGAAGGGAACAGATT+CGG	0.493759	6.3:+75766558	MS.gene05365:CDS
AGTATTGCAGAGGTATTTGA+AGG	0.515107	6.3:+75766459	MS.gene05365:CDS
CGAGTTTGATTCTGATTCGG+AGG	0.520197	6.3:+75766606	MS.gene05365:CDS
ATTTGATGAGGGATATTCTA+GGG	0.530573	6.3:+75766506	MS.gene05365:CDS
GATGAGGGATATTCTAGGGA+AGG	0.531728	6.3:+75766510	MS.gene05365:CDS
TGACGATGATGAGGTAGGGA+AGG	0.546633	6.3:+75766546	MS.gene05365:CDS
ATGAGGGATATTCTAGGGAA+GGG	0.555830	6.3:+75766511	MS.gene05365:CDS
TCGAATGACGATGATGAGGT+AGG	0.558012	6.3:+75766541	MS.gene05365:CDS
TTCATGTTAAAGAGTTGAAG+AGG	0.560555	6.3:+75766380	MS.gene05365:intron
AGTTGAAGAGGCGCGTTGCA+AGG	0.563749	6.3:+75766392	MS.gene05365:CDS
CGAATGACGATGATGAGGTA+GGG	0.565259	6.3:+75766542	MS.gene05365:CDS
TGCTGATCAGGATTTGATGA+GGG	0.581135	6.3:+75766495	MS.gene05365:CDS
ATGCTGATCAGGATTTGATG+AGG	0.589069	6.3:+75766494	MS.gene05365:CDS
CGCGTTGCAAGGCTTCGAGA+AGG	0.589292	6.3:+75766403	MS.gene05365:CDS
GACGATGATGAGGTAGGGAA+GGG	0.607551	6.3:+75766547	MS.gene05365:CDS
GAGTTTGATTCTGATTCGGA+GGG	0.613449	6.3:+75766607	MS.gene05365:CDS
AGATTCGGAAGACGATGATG+AGG	0.621691	6.3:+75766573	MS.gene05365:CDS
TATTGCAGAGGTATTTGAAG+GGG	0.623018	6.3:+75766461	MS.gene05365:CDS
TGAGGGATATTCTAGGGAAG+GGG	0.640498	6.3:+75766512	MS.gene05365:CDS
GTATTGCAGAGGTATTTGAA+GGG	0.641068	6.3:+75766460	MS.gene05365:CDS
TCTTTGAGGCAGTATTGCAG+AGG	0.647544	6.3:+75766449	MS.gene05365:CDS
AGAGTCGAATGACGATGATG+AGG	0.661123	6.3:+75766537	MS.gene05365:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TATATTGAATTAGTATTTTT+TGG	+	chr6.3:75766318-75766337	MS.gene05365:intron	10.0%
!!	ATATATTTGAATACTTGAAA+TGG	-	chr6.3:75766303-75766322	None:intergenic	15.0%
!!	TATATTTGAATACTTGAAAT+GGG	-	chr6.3:75766302-75766321	None:intergenic	15.0%
!!	ATATTTGAATACTTGAAATG+GGG	-	chr6.3:75766301-75766320	None:intergenic	20.0%
!!!	ATTTTTTGGTTTTTCAAATC+TGG	+	chr6.3:75766332-75766351	MS.gene05365:intron	20.0%
!	ATCATACCAAATACCAAATT+AGG	-	chr6.3:75766225-75766244	None:intergenic	25.0%
!!!	CAATTTTGAAAAACTCAGAA+AGG	-	chr6.3:75766162-75766181	None:intergenic	25.0%
!!!	CTTTCTGAGTTTTTCAAAAT+TGG	+	chr6.3:75766160-75766179	MS.gene05365:intron	25.0%
	ATTTGATGAGGGATATTCTA+GGG	+	chr6.3:75766506-75766525	MS.gene05365:CDS	30.0%
	TTCATGTTAAAGAGTTGAAG+AGG	+	chr6.3:75766380-75766399	MS.gene05365:intron	30.0%
!	TTTGAATACTAGTCTGAATG+AGG	-	chr6.3:75766126-75766145	None:intergenic	30.0%
	AAATCCTGATCAGCATCTAT+TGG	-	chr6.3:75766490-75766509	None:intergenic	35.0%
	AAGAGGCCTAATTTGGTATT+TGG	+	chr6.3:75766216-75766235	MS.gene05365:intron	35.0%
	AGACGAGTTTGATTCTGATT+CGG	+	chr6.3:75766603-75766622	MS.gene05365:CDS	35.0%
	CGTGATGTTAAAGCAAAAAG+CGG	+	chr6.3:75766670-75766689	MS.gene05365:CDS	35.0%
	GATTTGATGAGGGATATTCT+AGG	+	chr6.3:75766505-75766524	MS.gene05365:CDS	35.0%
	GTGATGTTAAAGCAAAAAGC+GGG	+	chr6.3:75766671-75766690	MS.gene05365:CDS	35.0%
	TGATGTTAAAGCAAAAAGCG+GGG	+	chr6.3:75766672-75766691	MS.gene05365:CDS	35.0%
!	TTTTTGTGTTCGTGTTGAAG+AGG	+	chr6.3:75766199-75766218	MS.gene05365:intron	35.0%
!!	AGTATTGCAGAGGTATTTGA+AGG	+	chr6.3:75766459-75766478	MS.gene05365:CDS	35.0%
!!	GTATTGCAGAGGTATTTGAA+GGG	+	chr6.3:75766460-75766479	MS.gene05365:CDS	35.0%
!!	TATTGCAGAGGTATTTGAAG+GGG	+	chr6.3:75766461-75766480	MS.gene05365:CDS	35.0%
	AATACTGCCTCAAAGACTTC+TGG	-	chr6.3:75766445-75766464	None:intergenic	40.0%
	ATGCTGATCAGGATTTGATG+AGG	+	chr6.3:75766494-75766513	MS.gene05365:CDS	40.0%
	TGCAATTCCAGAAGTCTTTG+AGG	+	chr6.3:75766435-75766454	MS.gene05365:CDS	40.0%
	TGCTGATCAGGATTTGATGA+GGG	+	chr6.3:75766495-75766514	MS.gene05365:CDS	40.0%
!	ATGAGGGATATTCTAGGGAA+GGG	+	chr6.3:75766511-75766530	MS.gene05365:CDS	40.0%
!!	CAGTGACTCTGATTTTGAAG+AGG	+	chr6.3:75766633-75766652	MS.gene05365:CDS	40.0%
!!	GAGTTTGATTCTGATTCGGA+GGG	+	chr6.3:75766607-75766626	MS.gene05365:CDS	40.0%
	AGAGTCGAATGACGATGATG+AGG	+	chr6.3:75766537-75766556	MS.gene05365:CDS	45.0%
	AGATTCGGAAGACGATGATG+AGG	+	chr6.3:75766573-75766592	MS.gene05365:CDS	45.0%
	CGAATGACGATGATGAGGTA+GGG	+	chr6.3:75766542-75766561	MS.gene05365:CDS	45.0%
	CGTGTTGAAGAGGCCTAATT+TGG	+	chr6.3:75766209-75766228	MS.gene05365:intron	45.0%
	GAAGCCAATAGATGCTGATC+AGG	+	chr6.3:75766483-75766502	MS.gene05365:CDS	45.0%
	TCGAATGACGATGATGAGGT+AGG	+	chr6.3:75766541-75766560	MS.gene05365:CDS	45.0%
!	GATGAGGGATATTCTAGGGA+AGG	+	chr6.3:75766510-75766529	MS.gene05365:CDS	45.0%
!	TGAGGGATATTCTAGGGAAG+GGG	+	chr6.3:75766512-75766531	MS.gene05365:CDS	45.0%
!!	CGAGTTTGATTCTGATTCGG+AGG	+	chr6.3:75766606-75766625	MS.gene05365:CDS	45.0%
!!	TCTTTGAGGCAGTATTGCAG+AGG	+	chr6.3:75766449-75766468	MS.gene05365:CDS	45.0%
	GACGATGATGAGGTAGGGAA+GGG	+	chr6.3:75766547-75766566	MS.gene05365:CDS	50.0%
	GGTAGGGAAGGGAACAGATT+CGG	+	chr6.3:75766558-75766577	MS.gene05365:CDS	50.0%
	TGACGATGATGAGGTAGGGA+AGG	+	chr6.3:75766546-75766565	MS.gene05365:CDS	50.0%
!	AGTTGAAGAGGCGCGTTGCA+AGG	+	chr6.3:75766392-75766411	MS.gene05365:CDS	55.0%
	CGCGTTGCAAGGCTTCGAGA+AGG	+	chr6.3:75766403-75766422	MS.gene05365:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.3	gene	75766112	75766711	75766112	ID=MS.gene05365
chr6.3	mRNA	75766112	75766711	75766112	ID=MS.gene05365.t1;Parent=MS.gene05365
chr6.3	exon	75766112	75766136	75766112	ID=MS.gene05365.t1.exon1;Parent=MS.gene05365.t1
chr6.3	CDS	75766112	75766136	75766112	ID=cds.MS.gene05365.t1;Parent=MS.gene05365.t1
chr6.3	exon	75766392	75766711	75766392	ID=MS.gene05365.t1.exon2;Parent=MS.gene05365.t1
chr6.3	CDS	75766392	75766711	75766392	ID=cds.MS.gene05365.t1;Parent=MS.gene05365.t1

Gene Sequence

>MS.gene05365

ATGTTTAAAAACCTCATTCAGACTAGTATTCAAATTTATTGAATTAGCCTTTCTGAGTTTTTCAAAATTGGTGTGAAGTTTTGATTTTTTTTGTGTTCGTGTTGAAGAGGCCTAATTTGGTATTTGGTATGATGCTTCTGTGAATTTCACAACACATCATTGTTTTTTCAAGTAATGTTGTAAAAACCCCATTTCAAGTATTCAAATATATTGAATTAGTATTTTTTGGTTTTTCAAATCTGGTGTGAAGTTTTGATTTGTATTTGTGTTCATGTTAAAGAGTTGAAGAGGCGCGTTGCAAGGCTTCGAGAAGGCGATGATGATGCAATTCCAGAAGTCTTTGAGGCAGTATTGCAGAGGTATTTGAAGGGGAAGCCAATAGATGCTGATCAGGATTTGATGAGGGATATTCTAGGGAAGGGGACAGAGTCGAATGACGATGATGAGGTAGGGAAGGGAACAGATTCGGAAGACGATGATGAGGAAAATGAAGACGAGTTTGATTCTGATTCGGAGGGAATCAGTGACTCTGATTTTGAAGAGGAAGAAGCTTTTAATCGTGATGTTAAAGCAAAAAGCGGGGTCAATTTAATGAAGTGA

Protein sequence

>MS.gene05365.t1

MFKNLIQTKLKRRVARLREGDDDAIPEVFEAVLQRYLKGKPIDADQDLMRDILGKGTESNDDDEVGKGTDSEDDDEENEDEFDSDSEGISDSDFEEEEAFNRDVKAKSGVNLMK