AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene96803

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96803.t1 MTR_4g081520 94.631 149 3 1 1 149 1 144 2.70e-99 281
MS.gene96803.t1 MTR_4g081510 69.231 130 33 2 6 135 1 123 1.04e-58 180
MS.gene96803.t1 MTR_7g099940 58.025 81 34 0 57 137 36 116 2.19e-28 102
MS.gene96803.t1 MTR_6g477870 50.000 96 48 0 40 135 79 174 1.84e-26 98.6
MS.gene96803.t1 MTR_7g105260 50.649 77 38 0 58 134 34 110 6.15e-22 85.5
MS.gene96803.t1 MTR_4g109540 41.837 98 52 1 44 136 25 122 1.93e-21 87.4
MS.gene96803.t1 MTR_3g025880 46.512 86 46 0 54 139 42 127 5.49e-21 88.2
MS.gene96803.t1 MTR_1g092860 45.000 80 44 0 58 137 194 273 2.44e-20 84.7
MS.gene96803.t1 MTR_7g013740 44.578 83 46 0 54 136 38 120 2.22e-19 79.3
MS.gene96803.t1 MTR_7g013730 42.353 85 49 0 55 139 39 123 5.15e-19 80.9
MS.gene96803.t1 MTR_4g109540 39.623 106 51 2 44 136 25 130 7.06e-19 80.9
MS.gene96803.t1 MTR_6g477870 46.341 82 44 0 40 121 79 160 2.63e-18 77.0
MS.gene96803.t1 MTR_3g084040 40.594 101 57 2 37 137 19 116 4.80e-18 75.9
MS.gene96803.t1 MTR_3g084040 40.698 86 48 2 37 122 19 101 1.86e-15 68.2
MS.gene96803.t1 MTR_3g029580 39.326 89 50 1 55 143 31 115 2.81e-15 68.2
MS.gene96803.t1 MTR_4g036415 39.796 98 59 0 35 132 430 527 6.38e-15 71.2
MS.gene96803.t1 MTR_4g070080 40.541 74 44 0 51 124 1 74 1.14e-14 66.6
MS.gene96803.t1 MTR_4g070080 40.541 74 44 0 51 124 1 74 2.39e-14 66.6
MS.gene96803.t1 MTR_4g070190 38.356 73 45 0 52 124 4 76 6.85e-14 65.5
MS.gene96803.t1 MTR_2g096560 38.750 80 49 0 56 135 7 86 1.33e-13 62.8
MS.gene96803.t1 MTR_3g462330 40.964 83 49 0 50 132 39 121 8.58e-13 64.7
MS.gene96803.t1 MTR_3g080130 41.463 82 46 2 56 136 36 116 2.03e-12 60.8
MS.gene96803.t1 MTR_4g070140 42.424 66 38 0 59 124 11 76 2.26e-12 62.0
MS.gene96803.t1 MTR_4g063560 28.182 110 71 1 33 134 2 111 4.58e-12 59.3
MS.gene96803.t1 MTR_3g075500 34.951 103 63 1 41 139 187 289 4.87e-12 62.4
MS.gene96803.t1 MTR_7g117495 41.667 60 35 0 56 115 18 77 7.45e-12 61.2
MS.gene96803.t1 MTR_7g117495 41.667 60 35 0 56 115 18 77 8.12e-12 61.2
MS.gene96803.t1 MTR_3g116130 39.080 87 47 2 56 138 29 113 8.25e-12 61.6
MS.gene96803.t1 MTR_3g116130 39.080 87 47 2 56 138 29 113 1.01e-11 61.6
MS.gene96803.t1 MTR_8g024520 43.478 69 35 1 56 120 25 93 1.22e-11 60.8
MS.gene96803.t1 MTR_8g024520 43.478 69 35 1 56 120 25 93 1.24e-11 60.8
MS.gene96803.t1 MTR_8g031560 32.787 122 72 2 22 135 159 278 2.43e-11 60.1
MS.gene96803.t1 MTR_8g070990 38.095 84 48 2 54 134 201 283 2.46e-11 60.8
MS.gene96803.t1 MTR_8g070990 38.095 84 48 2 54 134 201 283 2.61e-11 60.8
MS.gene96803.t1 MTR_8g070990 38.095 84 48 2 54 134 201 283 2.65e-11 60.8
MS.gene96803.t1 MTR_8g070990 38.095 84 48 2 54 134 308 390 2.69e-11 60.8
MS.gene96803.t1 MTR_8g070990 38.095 84 48 2 54 134 308 390 2.74e-11 60.8
MS.gene96803.t1 MTR_8g070990 38.095 84 48 2 54 134 308 390 2.78e-11 60.5
MS.gene96803.t1 MTR_7g094660 34.314 102 66 1 45 146 319 419 3.29e-11 60.5
MS.gene96803.t1 MTR_7g107580 35.417 96 54 1 54 149 346 433 3.64e-11 60.1
MS.gene96803.t1 MTR_7g107580 35.417 96 54 1 54 149 347 434 3.75e-11 60.1
MS.gene96803.t1 MTR_3g029580 42.424 66 38 0 55 120 31 96 4.11e-11 57.4
MS.gene96803.t1 MTR_2g026180 39.744 78 45 1 58 135 6 81 4.99e-11 59.7
MS.gene96803.t1 MTR_4g094235 32.609 92 57 1 42 133 7 93 5.31e-11 59.3
MS.gene96803.t1 MTR_4g094235 32.609 92 57 1 42 133 7 93 5.34e-11 59.3
MS.gene96803.t1 MTR_8g107310 35.294 102 55 1 45 135 189 290 5.71e-11 59.3
MS.gene96803.t1 MTR_1g045510 38.043 92 57 0 44 135 192 283 6.72e-11 58.9
MS.gene96803.t1 MTR_3g014000 35.484 93 49 2 54 139 32 120 7.24e-11 57.0
MS.gene96803.t1 MTR_5g070450 32.609 92 57 1 42 133 7 93 9.48e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96803.t1 AT5G54580 60.135 148 51 2 3 148 9 150 7.84e-53 164
MS.gene96803.t1 AT2G37510 63.014 73 27 0 58 130 36 108 2.64e-27 99.4
MS.gene96803.t1 AT1G73530 50.000 88 44 0 54 141 75 162 1.70e-25 95.9
MS.gene96803.t1 AT2G37510 46.939 98 27 1 58 130 36 133 2.10e-21 85.1
MS.gene96803.t1 AT5G06210 44.706 85 47 0 55 139 33 117 2.29e-19 79.3
MS.gene96803.t1 AT3G20930 40.244 82 49 0 55 136 281 362 3.25e-18 80.1
MS.gene96803.t1 AT5G61030 43.902 82 46 0 54 135 38 119 7.68e-18 78.6
MS.gene96803.t1 AT5G61030 43.902 82 46 0 54 135 38 119 7.68e-18 78.6
MS.gene96803.t1 AT3G46020 36.264 91 58 0 55 145 6 96 1.75e-16 70.5
MS.gene96803.t1 AT1G18630 48.611 72 37 0 56 127 36 107 2.16e-16 71.6
MS.gene96803.t1 AT4G13850 47.561 82 43 0 55 136 34 115 2.31e-16 71.6
MS.gene96803.t1 AT4G13850 44.330 97 51 1 40 136 22 115 2.34e-16 70.9
MS.gene96803.t1 AT3G23830 41.584 101 56 1 37 137 19 116 2.56e-16 71.2
MS.gene96803.t1 AT3G23830 41.584 101 56 1 37 137 19 116 2.56e-16 71.2
MS.gene96803.t1 AT4G13850 47.561 82 43 0 55 136 34 115 2.82e-16 71.6
MS.gene96803.t1 AT4G13850 47.561 82 43 0 55 136 34 115 3.14e-16 71.6
MS.gene96803.t1 AT3G20930 37.931 87 49 1 55 136 281 367 4.79e-16 73.9
MS.gene96803.t1 AT4G39260 39.326 89 54 0 51 139 1 89 1.40e-15 68.2
MS.gene96803.t1 AT2G43370 35.417 96 62 0 44 139 52 147 1.65e-15 70.5
MS.gene96803.t1 AT5G59865 43.750 80 45 0 56 135 26 105 2.24e-15 68.2
MS.gene96803.t1 AT2G43370 35.417 96 62 0 44 139 52 147 1.35e-14 69.7
MS.gene96803.t1 AT1G74230 43.590 78 43 1 55 132 33 109 3.34e-14 68.2
MS.gene96803.t1 AT4G13860 47.143 70 37 0 55 124 2 71 3.81e-14 64.3
MS.gene96803.t1 AT4G39260 41.558 77 45 0 51 127 1 77 7.07e-14 64.7
MS.gene96803.t1 AT5G47320 38.144 97 57 1 40 136 18 111 1.37e-13 65.5
MS.gene96803.t1 AT5G51300 34.234 111 68 2 22 132 451 556 1.43e-13 67.4
MS.gene96803.t1 AT5G51300 34.234 111 68 2 22 132 451 556 1.43e-13 67.4
MS.gene96803.t1 AT5G51300 34.234 111 68 2 22 132 451 556 1.43e-13 67.4
MS.gene96803.t1 AT4G39260 41.558 77 45 0 51 127 1 77 1.72e-13 64.7
MS.gene96803.t1 AT4G24770 36.000 100 64 0 38 137 226 325 3.22e-13 65.9
MS.gene96803.t1 AT4G24770 36.000 100 64 0 38 137 226 325 3.22e-13 65.9
MS.gene96803.t1 AT2G46780 39.535 86 48 1 56 137 22 107 3.73e-13 65.9
MS.gene96803.t1 AT2G46780 39.535 86 48 1 56 137 22 107 3.73e-13 65.9
MS.gene96803.t1 AT3G08000 40.698 86 51 0 57 142 42 127 5.53e-13 62.8
MS.gene96803.t1 AT3G26420 36.170 94 59 1 53 146 4 96 6.38e-13 64.3
MS.gene96803.t1 AT2G27330 29.787 94 64 1 55 146 20 113 1.39e-12 60.8
MS.gene96803.t1 AT1G76460 39.535 86 46 2 56 137 24 107 2.65e-12 63.2
MS.gene96803.t1 AT1G22330 36.471 85 48 2 56 136 17 99 2.75e-12 63.2
MS.gene96803.t1 AT3G53460 39.759 83 50 0 58 140 259 341 4.01e-12 62.8
MS.gene96803.t1 AT3G53460 39.759 83 50 0 58 140 259 341 4.01e-12 62.8
MS.gene96803.t1 AT3G53460 39.759 83 50 0 58 140 259 341 4.01e-12 62.8
MS.gene96803.t1 AT3G53460 39.759 83 50 0 58 140 280 362 4.10e-12 62.8
MS.gene96803.t1 AT1G07350 38.636 88 53 1 44 130 32 119 5.88e-12 59.7
MS.gene96803.t1 AT1G07350 38.636 88 53 1 44 130 32 119 5.88e-12 59.7
MS.gene96803.t1 AT2G37220 41.250 80 47 0 58 137 206 285 6.59e-12 62.0
MS.gene96803.t1 AT1G07350 38.750 80 49 0 53 132 72 151 6.96e-12 62.4
MS.gene96803.t1 AT2G23350 42.466 73 41 1 57 129 135 206 7.77e-12 62.4
MS.gene96803.t1 AT3G11400 33.051 118 63 2 35 136 203 320 8.53e-12 61.6
MS.gene96803.t1 AT1G07350 38.961 77 47 0 54 130 73 149 8.87e-12 59.7
MS.gene96803.t1 AT3G11400 33.051 118 63 2 35 136 176 293 1.02e-11 61.2
MS.gene96803.t1 AT4G34110 29.412 136 82 4 4 128 63 195 1.41e-11 61.6
MS.gene96803.t1 AT4G34110 37.838 74 43 2 56 127 215 287 7.70e-11 59.3
MS.gene96803.t1 AT5G64200 35.065 77 50 0 57 133 17 93 1.46e-11 60.8
MS.gene96803.t1 AT5G64200 35.065 77 50 0 57 133 17 93 1.46e-11 60.8
MS.gene96803.t1 AT1G60000 32.203 118 79 1 19 135 139 256 2.57e-11 60.1
MS.gene96803.t1 AT5G19030 31.579 76 52 0 59 134 80 155 2.78e-11 58.9
MS.gene96803.t1 AT5G47620 36.047 86 52 1 51 133 1 86 3.21e-11 60.5
MS.gene96803.t1 AT5G53720 41.558 77 38 2 51 120 1 77 3.36e-11 57.0
MS.gene96803.t1 AT2G21660 39.394 66 40 0 59 124 11 76 3.92e-11 58.2
MS.gene96803.t1 AT2G21660 39.394 66 40 0 59 124 11 76 5.18e-11 58.2
MS.gene96803.t1 AT4G20030 28.070 114 69 2 32 134 7 118 6.35e-11 57.4
MS.gene96803.t1 AT1G22910 47.273 55 29 0 56 110 13 67 7.68e-11 58.5
MS.gene96803.t1 AT1G22910 47.273 55 29 0 56 110 13 67 7.93e-11 58.5
MS.gene96803.t1 AT4G35785 36.364 88 56 0 50 137 66 153 8.38e-11 58.5
MS.gene96803.t1 AT4G26650 35.870 92 57 1 44 135 3 92 9.10e-11 58.9
MS.gene96803.t1 AT1G07350 37.363 91 56 1 44 133 32 122 9.36e-11 57.8

Find 55 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCATCATCGCCGCCTTC+TGG 0.178462 4.3:+28565464 MS.gene96803:CDS
GAACAACTTCACCGAACTTT+TGG 0.218023 4.3:-28566971 None:intergenic
TCAGGTTACTCTAAGGGATT+TGG 0.331272 4.3:+28567140 MS.gene96803:CDS
TCGAGTCTCAGGTTACTCTA+AGG 0.347313 4.3:+28567133 MS.gene96803:CDS
CGGATGAACATAAATTAATA+AGG 0.358256 4.3:-28568138 None:intergenic
GCTTCTCCGGGTGGTGGTCT+TGG 0.365700 4.3:-28568096 None:intergenic
CGTATTATTTACAGCTTCTC+CGG 0.378906 4.3:-28568109 None:intergenic
GAGAGCTGCAGCTGCGGCGG+CGG 0.386601 4.3:+28565376 MS.gene96803:CDS
AAGTTCGGTGAAGTTGTTCA+TGG 0.397028 4.3:+28566975 MS.gene96803:CDS
AGCACCAACCTCTTCGTCTC+TGG 0.450848 4.3:+28565521 MS.gene96803:CDS
CGGAGAAGCTGTAAATAATA+CGG 0.453875 4.3:+28568110 MS.gene96803:CDS
GATGCTGCTAGGGGCATTGA+AGG 0.455789 4.3:+28567188 MS.gene96803:CDS
AAAGAGTCGGCAGAAACTGC+GGG 0.478130 4.3:-28565402 None:intergenic
TACTTTAGAAGATGCTGCTA+GGG 0.485381 4.3:+28567178 MS.gene96803:CDS
AGGGAAGCATTCCAAAAGTT+CGG 0.494589 4.3:+28566960 MS.gene96803:CDS
ATCTGTCGAGCGGAAGGCGG+CGG 0.498886 4.3:-28565488 None:intergenic
TTTACAGCTTCTCCGGGTGG+TGG 0.499800 4.3:-28568102 None:intergenic
CTACTTTAGAAGATGCTGCT+AGG 0.502306 4.3:+28567177 MS.gene96803:CDS
GTGGTAACAGATCGAGTCTC+AGG 0.506975 4.3:+28567122 MS.gene96803:CDS
GCCGCCTTCCGCTCGACAGA+TGG 0.507477 4.3:+28565490 MS.gene96803:CDS
GATGAGAGCTGCAGCTGCGG+CGG 0.510313 4.3:+28565373 MS.gene96803:CDS
TGGAGAAGAAGCGGTTGGAT+TGG 0.515749 4.3:-28565429 None:intergenic
GATGAGAAAGAAGGGAAAGC+TGG 0.519875 4.3:-28565449 None:intergenic
GGCGGCGATGATGAGAAAGA+AGG 0.531889 4.3:-28565458 None:intergenic
CGAGTCTCAGGTTACTCTAA+GGG 0.537803 4.3:+28567134 MS.gene96803:CDS
GAAGAGGTTGGTGCTAGGCT+CGG 0.548156 4.3:-28565513 None:intergenic
GTATTATTTACAGCTTCTCC+GGG 0.550728 4.3:-28568108 None:intergenic
TCTCATCGTCAAAGCAGCCA+TGG 0.558291 4.3:-28565357 None:intergenic
GCGGAAGGCGGCGGTCCAGA+AGG 0.560349 4.3:-28565479 None:intergenic
GAAGGCGGCGGTCCAGAAGG+CGG 0.560594 4.3:-28565476 None:intergenic
TCGGCCATCTGTCGAGCGGA+AGG 0.561453 4.3:-28565494 None:intergenic
GACGATGAGAGCTGCAGCTG+CGG 0.562915 4.3:+28565370 MS.gene96803:CDS
GCCATCTGTCGAGCGGAAGG+CGG 0.576022 4.3:-28565491 None:intergenic
TGAACATAAATTAATAAGGT+CGG 0.576922 4.3:-28568134 None:intergenic
TGCTAGGGGCATTGAAGGAA+TGG 0.581040 4.3:+28567193 MS.gene96803:CDS
CTCATCGTCAAAGCAGCCAT+GGG 0.581556 4.3:-28565356 None:intergenic
GCGGCGATGATGAGAAAGAA+GGG 0.588249 4.3:-28565457 None:intergenic
GGAGAAGCTGTAAATAATAC+GGG 0.589568 4.3:+28568111 MS.gene96803:CDS
AAAGCTGGAGAAGAAGCGGT+TGG 0.590957 4.3:-28565434 None:intergenic
GAGACGAAGAGGTTGGTGCT+AGG 0.593582 4.3:-28565518 None:intergenic
CGAACCAGAGACGAAGAGGT+TGG 0.593995 4.3:-28565525 None:intergenic
AGGGGCATTGAAGGAATGGA+TGG 0.598515 4.3:+28567197 MS.gene96803:CDS
AGGGAAAGCTGGAGAAGAAG+CGG 0.611069 4.3:-28565438 None:intergenic
TTGGATTGGTGCAAAAGAGT+CGG 0.611688 4.3:-28565415 None:intergenic
AAAAGAGTCGGCAGAAACTG+CGG 0.620147 4.3:-28565403 None:intergenic
TTATTTACAGCTTCTCCGGG+TGG 0.622983 4.3:-28568105 None:intergenic
CATTGAAGGAATGGATGGCA+AGG 0.640049 4.3:+28567202 MS.gene96803:CDS
AAGGCGAACCAGAGACGAAG+AGG 0.640180 4.3:-28565529 None:intergenic
TTTGTCAATCTCAGCAAGGG+TGG 0.647632 4.3:+28567103 MS.gene96803:intron
GTACTACAACAGAGACGCTG+AGG 0.653561 4.3:+28566940 MS.gene96803:CDS
AGGCTCGGCCATCTGTCGAG+CGG 0.658207 4.3:-28565498 None:intergenic
GCAAGACCAAGACCACCACC+CGG 0.665523 4.3:+28568090 MS.gene96803:CDS
TACTACAACAGAGACGCTGA+GGG 0.682055 4.3:+28566941 MS.gene96803:CDS
ACTTTAGAAGATGCTGCTAG+GGG 0.684689 4.3:+28567179 MS.gene96803:CDS
AAGAGTCGGCAGAAACTGCG+GGG 0.717765 4.3:-28565401 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTATAATAATAATAATA+AGG - chr4.3:28565571-28565590 None:intergenic 0.0%
!!! AATTTATTTATTTTTATTTA+TGG - chr4.3:28566810-28566829 None:intergenic 0.0%
!! ATTATAATTATTATTGTTGT+TGG + chr4.3:28565581-28565600 MS.gene96803:intron 10.0%
!!! TTTTTTATAATCCTTAAAAA+CGG - chr4.3:28565748-28565767 None:intergenic 10.0%
!!! ATTTATTTTTATTTATGGCA+TGG - chr4.3:28566805-28566824 None:intergenic 15.0%
!!! TTTATTTTTATTTATGGCAT+GGG - chr4.3:28566804-28566823 None:intergenic 15.0%
!! AGTTTAAATGTTTAAAAGGA+TGG - chr4.3:28567009-28567028 None:intergenic 20.0%
!! CAAAAAACATAAAACCATTA+TGG - chr4.3:28567087-28567106 None:intergenic 20.0%
!! GAACAGTTTAAATGTTTAAA+AGG - chr4.3:28567013-28567032 None:intergenic 20.0%
!! TAAATTAAGTTTATCTTAGG+TGG - chr4.3:28567548-28567567 None:intergenic 20.0%
!! TATTATAAAGACATACAATG+AGG - chr4.3:28567506-28567525 None:intergenic 20.0%
!! TGTTTAAGCAATAAGTAAAT+AGG - chr4.3:28565950-28565969 None:intergenic 20.0%
!! TTTATCAATTTCATCATAGA+AGG + chr4.3:28566178-28566197 MS.gene96803:intron 20.0%
!!! ACAAATTGGTTTTGTAAATA+AGG + chr4.3:28566209-28566228 MS.gene96803:intron 20.0%
!!! ACATTGTTATTGTATATGTA+TGG + chr4.3:28567921-28567940 MS.gene96803:intron 20.0%
!!! TTACAAAACCAATTTGTTAA+AGG - chr4.3:28566206-28566225 None:intergenic 20.0%
!!! TTATTTTTATTTATGGCATG+GGG - chr4.3:28566803-28566822 None:intergenic 20.0%
! AAAATTGCAAATGACAATTG+AGG - chr4.3:28566769-28566788 None:intergenic 25.0%
! AAATTAAGAGAATTGAATCC+TGG - chr4.3:28566719-28566738 None:intergenic 25.0%
! AATACATGATTTACAGATTC+GGG - chr4.3:28567804-28567823 None:intergenic 25.0%
! AATTAAGAGAATTGAATCCT+GGG - chr4.3:28566718-28566737 None:intergenic 25.0%
! ACGATATAAGTAGAAATAAG+AGG + chr4.3:28566319-28566338 MS.gene96803:intron 25.0%
! AGAAAATATACTGTATGAGT+TGG + chr4.3:28566257-28566276 MS.gene96803:intron 25.0%
! ATAATAAGGAAAAGTTGAGA+AGG - chr4.3:28565557-28565576 None:intergenic 25.0%
! CAATACATGATTTACAGATT+CGG - chr4.3:28567805-28567824 None:intergenic 25.0%
! CAATATTTGAATAGGAAGTT+TGG - chr4.3:28568007-28568026 None:intergenic 25.0%
! CGATATAAGTAGAAATAAGA+GGG + chr4.3:28566320-28566339 MS.gene96803:intron 25.0%
! CGGTAAATTAAGTTTATCTT+AGG - chr4.3:28567551-28567570 None:intergenic 25.0%
! GATATAAGTAGAAATAAGAG+GGG + chr4.3:28566321-28566340 MS.gene96803:intron 25.0%
! GTAAGATTTATTAAACCCTT+TGG + chr4.3:28567224-28567243 MS.gene96803:intron 25.0%
! TAATAAGGAAAAGTTGAGAA+GGG - chr4.3:28565556-28565575 None:intergenic 25.0%
! TACTAATATCAAACCACAAA+GGG - chr4.3:28566034-28566053 None:intergenic 25.0%
! TACTCTAAATATTGAAGAGT+TGG - chr4.3:28567768-28567787 None:intergenic 25.0%
! TCAGTAAGCAATATTTGAAT+AGG - chr4.3:28568015-28568034 None:intergenic 25.0%
! TCTTAAAGCACAAATTTGTA+TGG + chr4.3:28567646-28567665 MS.gene96803:intron 25.0%
! TTACTAATATCAAACCACAA+AGG - chr4.3:28566035-28566054 None:intergenic 25.0%
! TTTATGTTCTCATTTGATCT+TGG + chr4.3:28565706-28565725 MS.gene96803:intron 25.0%
!! ACATAAAACCATTATGGTTT+TGG - chr4.3:28567081-28567100 None:intergenic 25.0%
!! ATAAATTTCTTTTCCCTTTG+TGG + chr4.3:28566018-28566037 MS.gene96803:intron 25.0%
!! TCAAAGAAATATGTTTCTGT+TGG - chr4.3:28566529-28566548 None:intergenic 25.0%
AAGAACAGTGAATACATTGT+TGG - chr4.3:28567630-28567649 None:intergenic 30.0%
AAGGATCATTTCATAATCCT+CGG + chr4.3:28566442-28566461 MS.gene96803:intron 30.0%
AATCTACTCCAAAACCATAA+TGG + chr4.3:28567070-28567089 MS.gene96803:intron 30.0%
ATACATGATTTACAGATTCG+GGG - chr4.3:28567803-28567822 None:intergenic 30.0%
CTTTAATACACTTAATTCCC+AGG + chr4.3:28566698-28566717 MS.gene96803:intron 30.0%
GGATCATTAAATTTGATGTC+AGG - chr4.3:28567747-28567766 None:intergenic 30.0%
GGGAAAAGAAATTTATACAC+AGG - chr4.3:28566014-28566033 None:intergenic 30.0%
TCATTAAATTTGATGTCAGG+AGG - chr4.3:28567744-28567763 None:intergenic 30.0%
! AGACTTAAGTGTATTTCTGT+CGG + chr4.3:28567269-28567288 MS.gene96803:intron 30.0%
! ATGCAATTTAGAGCAAGTAT+GGG - chr4.3:28566630-28566649 None:intergenic 30.0%
! TTGTTATCTTGGATCTGTTT+TGG + chr4.3:28567296-28567315 MS.gene96803:intron 30.0%
!! TTATGTTTTTGACCATGTTG+AGG + chr4.3:28565671-28565690 MS.gene96803:intron 30.0%
!!! TTTTTTGTCAATCTCAGCAA+GGG + chr4.3:28567100-28567119 MS.gene96803:intron 30.0%
AAATTTGATGTCAGGAGGAA+GGG - chr4.3:28567739-28567758 None:intergenic 35.0%
AGAAGGAGCCTTTAACAAAT+TGG + chr4.3:28566195-28566214 MS.gene96803:intron 35.0%
ATGCAACTGACAACAAAACT+CGG - chr4.3:28567878-28567897 None:intergenic 35.0%
CACTTAAGTCTTCTCGTATA+AGG - chr4.3:28567260-28567279 None:intergenic 35.0%
CATCATATGAAACCTCAACA+TGG - chr4.3:28565686-28565705 None:intergenic 35.0%
CGGAGAAGCTGTAAATAATA+CGG + chr4.3:28568110-28568129 MS.gene96803:CDS 35.0%
CGTATTATTTACAGCTTCTC+CGG - chr4.3:28568112-28568131 None:intergenic 35.0%
CTCATTTGATCTTGGTTGTT+TGG + chr4.3:28565714-28565733 MS.gene96803:intron 35.0%
CTCCAAAAACTGTATTGCAA+GGG - chr4.3:28568046-28568065 None:intergenic 35.0%
GACACAGTCAAGAACTTAAA+AGG - chr4.3:28566354-28566373 None:intergenic 35.0%
GGAGAAGCTGTAAATAATAC+GGG + chr4.3:28568111-28568130 MS.gene96803:CDS 35.0%
GTAAATCTATCTCACTCTCA+CGG - chr4.3:28566122-28566141 None:intergenic 35.0%
GTATTATTTACAGCTTCTCC+GGG - chr4.3:28568111-28568130 None:intergenic 35.0%
TAAATCTATCTCACTCTCAC+GGG - chr4.3:28566121-28566140 None:intergenic 35.0%
TAAATTTGATGTCAGGAGGA+AGG - chr4.3:28567740-28567759 None:intergenic 35.0%
TGACGTGTCTTAAACTTTCA+GGG + chr4.3:28566907-28566926 MS.gene96803:intron 35.0%
TGATCTTGGTTGTTTGGTTA+TGG + chr4.3:28565720-28565739 MS.gene96803:intron 35.0%
TTGACGTGTCTTAAACTTTC+AGG + chr4.3:28566906-28566925 MS.gene96803:intron 35.0%
TTTATTTATGGCATGGGGAA+GGG - chr4.3:28566798-28566817 None:intergenic 35.0%
! AATGACAATTGAGGCATCAA+TGG - chr4.3:28566760-28566779 None:intergenic 35.0%
! CATACTTGCTCTAAATTGCA+TGG + chr4.3:28566629-28566648 MS.gene96803:intron 35.0%
! CATGCAATTTAGAGCAAGTA+TGG - chr4.3:28566631-28566650 None:intergenic 35.0%
! TACTTTAGAAGATGCTGCTA+GGG + chr4.3:28567178-28567197 MS.gene96803:CDS 35.0%
! TTCCCTTGCAATACAGTTTT+TGG + chr4.3:28568041-28568060 MS.gene96803:intron 35.0%
!! CTAGAGAAAGCATCTAAGAA+AGG + chr4.3:28566423-28566442 MS.gene96803:intron 35.0%
!! CTTGCAATACAGTTTTTGGA+GGG + chr4.3:28568045-28568064 MS.gene96803:intron 35.0%
!! TGGTTATGGATCCGTTTTTA+AGG + chr4.3:28565734-28565753 MS.gene96803:intron 35.0%
!! TTTTATTTATGGCATGGGGA+AGG - chr4.3:28566799-28566818 None:intergenic 35.0%
!!! GTTTTTTGTCAATCTCAGCA+AGG + chr4.3:28567099-28567118 MS.gene96803:intron 35.0%
AACACTTGTGTGTCACATTG+TGG + chr4.3:28567431-28567450 MS.gene96803:intron 40.0%
AATTTGATGTCAGGAGGAAG+GGG - chr4.3:28567738-28567757 None:intergenic 40.0%
ACAATGTGACACACGGATTT+CGG + chr4.3:28567381-28567400 MS.gene96803:intron 40.0%
AGGAAAAGTTGAGAAGGGAA+AGG - chr4.3:28565551-28565570 None:intergenic 40.0%
AGTGTGATGTGTATATGCTG+CGG - chr4.3:28567577-28567596 None:intergenic 40.0%
ATTTCACTTGTGTGTCATCG+TGG + chr4.3:28567330-28567349 MS.gene96803:intron 40.0%
CAATGGCATGTTGTTAAGAG+AGG - chr4.3:28566743-28566762 None:intergenic 40.0%
CCTCCAAAAACTGTATTGCA+AGG - chr4.3:28568047-28568066 None:intergenic 40.0%
CTCAAAAGAGACCTACAGTA+CGG + chr4.3:28566502-28566521 MS.gene96803:intron 40.0%
CTGTCGGAACATTGTTATCT+TGG + chr4.3:28567285-28567304 MS.gene96803:intron 40.0%
TCTCACGGGTAGTAAAACTA+AGG - chr4.3:28566107-28566126 None:intergenic 40.0%
TTTACTGCAAACTCGAGTAG+AGG - chr4.3:28567673-28567692 None:intergenic 40.0%
! AAGTTCGGTGAAGTTGTTCA+TGG + chr4.3:28566975-28566994 MS.gene96803:CDS 40.0%
! ACTTTAGAAGATGCTGCTAG+GGG + chr4.3:28567179-28567198 MS.gene96803:CDS 40.0%
! ATTTCGGTCTTGTGAAAGAG+AGG + chr4.3:28567397-28567416 MS.gene96803:intron 40.0%
! CTACTTTAGAAGATGCTGCT+AGG + chr4.3:28567177-28567196 MS.gene96803:CDS 40.0%
! GAACAACTTCACCGAACTTT+TGG - chr4.3:28566974-28566993 None:intergenic 40.0%
! TCAGGTTACTCTAAGGGATT+TGG + chr4.3:28567140-28567159 MS.gene96803:CDS 40.0%
! TTTCGGTCTTGTGAAAGAGA+GGG + chr4.3:28567398-28567417 MS.gene96803:intron 40.0%
!! AATACAGTTTTTGGAGGGCT+GGG + chr4.3:28568050-28568069 MS.gene96803:intron 40.0%
!! AGGGAAGCATTCCAAAAGTT+CGG + chr4.3:28566960-28566979 MS.gene96803:CDS 40.0%
!! CCTTGCAATACAGTTTTTGG+AGG + chr4.3:28568044-28568063 MS.gene96803:intron 40.0%
!! GTTTTCGCTTTTGAGCCAAA+CGG - chr4.3:28566679-28566698 None:intergenic 40.0%
!! TTGGATTGGTGCAAAAGAGT+CGG - chr4.3:28565418-28565437 None:intergenic 40.0%
AAAAGAGTCGGCAGAAACTG+CGG - chr4.3:28565406-28565425 None:intergenic 45.0%
AGTCTTCTCGTATAAGGTGC+AGG - chr4.3:28567254-28567273 None:intergenic 45.0%
ATCCCACTTGTGTGACATTG+TGG - chr4.3:28567483-28567502 None:intergenic 45.0%
ATGTGTATATGCTGCGGTGT+CGG - chr4.3:28567571-28567590 None:intergenic 45.0%
CACCACAATGTCACACAAGT+GGG + chr4.3:28567478-28567497 MS.gene96803:intron 45.0%
CACTTGTGTGACATTGTGGT+GGG - chr4.3:28567479-28567498 None:intergenic 45.0%
CATTGAAGGAATGGATGGCA+AGG + chr4.3:28567202-28567221 MS.gene96803:CDS 45.0%
CGAGTCTCAGGTTACTCTAA+GGG + chr4.3:28567134-28567153 MS.gene96803:CDS 45.0%
CTTGTGTGTCACATTGTGGT+TGG + chr4.3:28567435-28567454 MS.gene96803:intron 45.0%
GAAATCCGTGTGTCACATTG+TGG - chr4.3:28567382-28567401 None:intergenic 45.0%
GATGAGAAAGAAGGGAAAGC+TGG - chr4.3:28565452-28565471 None:intergenic 45.0%
TACTACAACAGAGACGCTGA+GGG + chr4.3:28566941-28566960 MS.gene96803:CDS 45.0%
TCGAGTCTCAGGTTACTCTA+AGG + chr4.3:28567133-28567152 MS.gene96803:CDS 45.0%
TTATTTACAGCTTCTCCGGG+TGG - chr4.3:28568108-28568127 None:intergenic 45.0%
TTTCTGTTGGACCGTACTGT+AGG - chr4.3:28566516-28566535 None:intergenic 45.0%
TTTGTCAATCTCAGCAAGGG+TGG + chr4.3:28567103-28567122 MS.gene96803:intron 45.0%
! ATTGTGGTGGGAATGTCACA+TGG - chr4.3:28567467-28567486 None:intergenic 45.0%
!! CAATACAGTTTTTGGAGGGC+TGG + chr4.3:28568049-28568068 MS.gene96803:intron 45.0%
!! TTCAAAGCACTGTGACCGTT+TGG + chr4.3:28566661-28566680 MS.gene96803:intron 45.0%
AAAGAGTCGGCAGAAACTGC+GGG - chr4.3:28565405-28565424 None:intergenic 50.0%
AAAGCTGGAGAAGAAGCGGT+TGG - chr4.3:28565437-28565456 None:intergenic 50.0%
ACTTGTGTGTCATCGTGGTG+GGG + chr4.3:28567335-28567354 MS.gene96803:intron 50.0%
AGGGAAAGCTGGAGAAGAAG+CGG - chr4.3:28565441-28565460 None:intergenic 50.0%
ATTTATGGCATGGGGAAGGG+AGG - chr4.3:28566795-28566814 None:intergenic 50.0%
CACTTGTGTGTCATCGTGGT+GGG + chr4.3:28567334-28567353 MS.gene96803:intron 50.0%
CCACCACAATGTCACACAAG+TGG + chr4.3:28567477-28567496 MS.gene96803:intron 50.0%
CCACTTGTGTGACATTGTGG+TGG - chr4.3:28567480-28567499 None:intergenic 50.0%
CCCAACCACAATGTGACACA+CGG + chr4.3:28567374-28567393 MS.gene96803:intron 50.0%
CCGTGTGTCACATTGTGGTT+GGG - chr4.3:28567377-28567396 None:intergenic 50.0%
GCGGCGATGATGAGAAAGAA+GGG - chr4.3:28565460-28565479 None:intergenic 50.0%
GTACTACAACAGAGACGCTG+AGG + chr4.3:28566940-28566959 MS.gene96803:CDS 50.0%
GTGGTAACAGATCGAGTCTC+AGG + chr4.3:28567122-28567141 MS.gene96803:CDS 50.0%
TCACTTGTGTGTCATCGTGG+TGG + chr4.3:28567333-28567352 MS.gene96803:intron 50.0%
TCCGTGTGTCACATTGTGGT+TGG - chr4.3:28567378-28567397 None:intergenic 50.0%
TGGAGAAGAAGCGGTTGGAT+TGG - chr4.3:28565432-28565451 None:intergenic 50.0%
TTCGAGTAGACACTTGACCG+AGG - chr4.3:28566462-28566481 None:intergenic 50.0%
TTCTCGTATAAGGTGCAGGC+AGG - chr4.3:28567250-28567269 None:intergenic 50.0%
!! AGGGGCATTGAAGGAATGGA+TGG + chr4.3:28567197-28567216 MS.gene96803:CDS 50.0%
!! TGCTAGGGGCATTGAAGGAA+TGG + chr4.3:28567193-28567212 MS.gene96803:CDS 50.0%
AAGAGTCGGCAGAAACTGCG+GGG - chr4.3:28565404-28565423 None:intergenic 55.0%
AAGGCGAACCAGAGACGAAG+AGG - chr4.3:28565532-28565551 None:intergenic 55.0%
AGCACCAACCTCTTCGTCTC+TGG + chr4.3:28565521-28565540 MS.gene96803:CDS 55.0%
ATAAGGTGCAGGCAGGCCAA+AGG - chr4.3:28567243-28567262 None:intergenic 55.0%
CGAACCAGAGACGAAGAGGT+TGG - chr4.3:28565528-28565547 None:intergenic 55.0%
GGCGGCGATGATGAGAAAGA+AGG - chr4.3:28565461-28565480 None:intergenic 55.0%
TAAGGTGCAGGCAGGCCAAA+GGG - chr4.3:28567242-28567261 None:intergenic 55.0%
TTCTCATCATCGCCGCCTTC+TGG + chr4.3:28565464-28565483 MS.gene96803:CDS 55.0%
TTTACAGCTTCTCCGGGTGG+TGG - chr4.3:28568105-28568124 None:intergenic 55.0%
! CATCGTGGTGGGGATTTCAC+AGG + chr4.3:28567345-28567364 MS.gene96803:intron 55.0%
!! GAAGAGGTTGGTGCTAGGCT+CGG - chr4.3:28565516-28565535 None:intergenic 55.0%
!! GAGACGAAGAGGTTGGTGCT+AGG - chr4.3:28565521-28565540 None:intergenic 55.0%
!! GATGCTGCTAGGGGCATTGA+AGG + chr4.3:28567188-28567207 MS.gene96803:CDS 55.0%
GACGATGAGAGCTGCAGCTG+CGG + chr4.3:28565370-28565389 MS.gene96803:CDS 60.0%
GCAAGACCAAGACCACCACC+CGG + chr4.3:28568090-28568109 MS.gene96803:CDS 60.0%
AGGCTCGGCCATCTGTCGAG+CGG - chr4.3:28565501-28565520 None:intergenic 65.0%
ATCTGTCGAGCGGAAGGCGG+CGG - chr4.3:28565491-28565510 None:intergenic 65.0%
GATGAGAGCTGCAGCTGCGG+CGG + chr4.3:28565373-28565392 MS.gene96803:CDS 65.0%
GCCATCTGTCGAGCGGAAGG+CGG - chr4.3:28565494-28565513 None:intergenic 65.0%
TCGGCCATCTGTCGAGCGGA+AGG - chr4.3:28565497-28565516 None:intergenic 65.0%
! GCTTCTCCGGGTGGTGGTCT+TGG - chr4.3:28568099-28568118 None:intergenic 65.0%
GAAGGCGGCGGTCCAGAAGG+CGG - chr4.3:28565479-28565498 None:intergenic 70.0%
GCCGCCTTCCGCTCGACAGA+TGG + chr4.3:28565490-28565509 MS.gene96803:CDS 70.0%
GAGAGCTGCAGCTGCGGCGG+CGG + chr4.3:28565376-28565395 MS.gene96803:CDS 75.0%
GCGGAAGGCGGCGGTCCAGA+AGG - chr4.3:28565482-28565501 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 28565359 28568146 28565359 ID=MS.gene96803
chr4.3 mRNA 28565359 28568146 28565359 ID=MS.gene96803.t1;Parent=MS.gene96803
chr4.3 exon 28565359 28565542 28565359 ID=MS.gene96803.t1.exon1;Parent=MS.gene96803.t1
chr4.3 CDS 28565359 28565542 28565359 ID=cds.MS.gene96803.t1;Parent=MS.gene96803.t1
chr4.3 exon 28566928 28566996 28566928 ID=MS.gene96803.t1.exon2;Parent=MS.gene96803.t1
chr4.3 CDS 28566928 28566996 28566928 ID=cds.MS.gene96803.t1;Parent=MS.gene96803.t1
chr4.3 exon 28567117 28567223 28567117 ID=MS.gene96803.t1.exon3;Parent=MS.gene96803.t1
chr4.3 CDS 28567117 28567223 28567117 ID=cds.MS.gene96803.t1;Parent=MS.gene96803.t1
chr4.3 exon 28568057 28568146 28568057 ID=MS.gene96803.t1.exon4;Parent=MS.gene96803.t1
chr4.3 CDS 28568057 28568146 28568057 ID=cds.MS.gene96803.t1;Parent=MS.gene96803.t1
Gene Sequence

>MS.gene96803

ATGGCTGCTTTGACGATGAGAGCTGCAGCTGCGGCGGCGGTGCCCCGCAGTTTCTGCCGACTCTTTTGCACCAATCCAACCGCTTCTTCTCCAGCTTTCCCTTCTTTCTCATCATCGCCGCCTTCTGGACCGCCGCCTTCCGCTCGACAGATGGCCGAGCCTAGCACCAACCTCTTCGTCTCTGGTTCGCCTTTCCCTTCTCAACTTTTCCTTATTATTATTATTATAATTATTATTGTTGTTGGTTGAATGCTAGATAGTTATTGTGATTTATGAATTGAAAGTGTATGTTTTGATATTATTGTTTGTGTTTTATGTTTTTGACCATGTTGAGGTTTCATATGATGTTTATGTTCTCATTTGATCTTGGTTGTTTGGTTATGGATCCGTTTTTAAGGATTATAAAAAAGTGATTTTGATACTCAATTATTTAGATATTATTTTTTAGAGATTTTCAATAAACGCATAATCTGTTTTGTGTTTGTTTTACAAAGTTTAAATGAAAATTTATAGAATTGATTTTTTTTTTTTAATGAAAAATCAGAATTTTCAATCAAATACACAGATGCTATTAAAGCTGTTGAATTTCCTATTTACTTATTGCTTAAACAACTTATCTTTGTTTAAAATAACAGCTTATGCTTATGCATTTCCTGTGTATAAATTTCTTTTCCCTTTGTGGTTTGATATTAGTAATTCTTTGAATAAGTGATTTGTTTTCATGTATTCAATTCATAAGTAGACTCCTTAGTTTTACTACCCGTGAGAGTGAGATAGATTTACATATATGACATCTGTACACTCATGCAAAAAGTAATTTTTATCAATTTCATCATAGAAGGAGCCTTTAACAAATTGGTTTTGTAAATAAGGAAGCTTTTGAAATGATTAATTAACTAGAAAATATACTGTATGAGTTGGAACTCGCGACTCTTTTATCTGTATTGCTAGATAAATCAAACGATATAAGTAGAAATAAGAGGGGATTTTATCCTTTTAAGTTCTTGACTGTGTCTATAGAAATGTTTAGTTGCGTAAAGTAAAAATTAGATAGATAATATAATCTAGAGAAAGCATCTAAGAAAGGATCATTTCATAATCCTCGGTCAAGTGTCTACTCGAAATATGAGTATTGTCATCAAACTCAAAAGAGACCTACAGTACGGTCCAACAGAAACATATTTCTTTGAAGCAATCTCTGAAAATACGAGTGCTTTTCACATTTTTTGATTGCAGTGTTGAATCACTACTAGCTATTTTGATTTTAACCCATACTTGCTCTAAATTGCATGGTTTCGTGACTTCAAAGCACTGTGACCGTTTGGCTCAAAAGCGAAAACTTTAATACACTTAATTCCCAGGATTCAATTCTCTTAATTTTCCTCTCTTAACAACATGCCATTGATGCCTCAATTGTCATTTGCAATTTTTATCCTCCCTTCCCCATGCCATAAATAAAAATAAATAAATTTGTATATGTCATTGCTAATGTTTAGCTTTATTTCAATGCAATTTGTCACTTGAAATATTCAGTTGACTGTTGATTTTTGACGTGTCTTAAACTTTCAGGGCTTAGTAAACGTACTACAACAGAGACGCTGAGGGAAGCATTCCAAAAGTTCGGTGAAGTTGTTCATGGTTTGTTCTCCATCCTTTTAAACATTTAAACTGTTCTGCTATTTATGCTTTTGCTATGCAATGATGACTTGAAAATCTACTCCAAAACCATAATGGTTTTATGTTTTTTGTCAATCTCAGCAAGGGTGGTAACAGATCGAGTCTCAGGTTACTCTAAGGGATTTGGTTTCGTGAAATATGCTACTTTAGAAGATGCTGCTAGGGGCATTGAAGGAATGGATGGCAAGGTAAGATTTATTAAACCCTTTGGCCTGCCTGCACCTTATACGAGAAGACTTAAGTGTATTTCTGTCGGAACATTGTTATCTTGGATCTGTTTTGGATTTTTCACTGATTTCACTTGTGTGTCATCGTGGTGGGGATTTCACAGGACATTGCCCAACCACAATGTGACACACGGATTTCGGTCTTGTGAAAGAGAGGGAAGAAAACAAAACACTTGTGTGTCACATTGTGGTTGGACAATGCCATGTGACATTCCCACCACAATGTCACACAAGTGGGATCCTCATTGTATGTCTTTATAATAAAAAGTTAGTTCAGTATGACCACCTAAGATAAACTTAATTTACCGACACCGCAGCATATACACATCACACTAGAAGATTGAACTCTAATTGCTTGACATTGCCAACAATGTATTCACTGTTCTTAAAGCACAAATTTGTATGGACCTCTACTCGAGTTTGCAGTAAATCGCATTTACTGTTATTTGAACTCAAGTTTTCTCAATTTTCTCCCCTTCCTCCTGACATCAAATTTAATGATCCAACTCTTCAATATTTAGAGTATTTGCTTAATCACCCCGAATCTGTAAATCATGTATTGATTGCTGAGCTCAAATAAATATGCATTGTTTGACTTAACAACTGCTATAACCGAGTTTTGTTGTCAGTTGCATGTTGTTTTAATGAATTTTTCTTAACATTGTTATTGTATATGTATGGAAAACTTTCTTATGTAGTTCATCTACACAATTGATGCATGATTGTTCTTTGCTTTTCTTGCCAAACTTCCTATTCAAATATTGCTTACTGATATGTTTTCCCTTGCAATACAGTTTTTGGAGGGCTGGGTTATATTTGCAGAGTATGCAAGACCAAGACCACCACCCGGAGAAGCTGTAAATAATACGGGCCGACCTTATTAA
Protein sequence

>MS.gene96803.t1

MAALTMRAAAAAAVPRSFCRLFCTNPTASSPAFPSFSSSPPSGPPPSARQMAEPSTNLFVSGLSKRTTTETLREAFQKFGEVVHARVVTDRVSGYSKGFGFVKYATLEDAARGIEGMDGKFLEGWVIFAEYARPRPPPGEAVNNTGRPY