AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene96804

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96804.t1 MTR_4g081510 87.027 185 19 2 1 184 1 181 1.92e-111 315
MS.gene96804.t1 MTR_4g081520 73.950 119 28 1 9 127 15 130 4.76e-52 164
MS.gene96804.t1 MTR_6g477870 48.101 79 41 0 50 128 97 175 4.74e-24 93.6
MS.gene96804.t1 MTR_7g099940 51.282 78 38 0 50 127 37 114 6.68e-24 92.0
MS.gene96804.t1 MTR_7g105260 46.835 79 42 0 50 128 34 112 1.31e-19 80.9
MS.gene96804.t1 MTR_1g092860 39.362 94 50 2 50 140 194 283 3.91e-18 80.1
MS.gene96804.t1 MTR_7g013740 46.914 81 41 1 50 128 42 122 7.90e-18 76.3
MS.gene96804.t1 MTR_4g109540 37.736 106 62 2 27 128 19 124 8.81e-17 76.3
MS.gene96804.t1 MTR_3g025880 45.977 87 45 1 43 127 39 125 9.00e-17 77.4
MS.gene96804.t1 MTR_7g013730 43.590 78 44 0 50 127 42 119 1.38e-16 75.5
MS.gene96804.t1 MTR_6g477870 46.875 64 34 0 50 113 97 160 3.74e-16 72.4
MS.gene96804.t1 MTR_4g109540 43.210 81 44 1 50 128 52 132 3.71e-15 71.6
MS.gene96804.t1 MTR_3g084040 42.308 78 44 1 50 127 38 114 1.26e-14 67.8
MS.gene96804.t1 MTR_3g029580 41.558 77 45 0 50 126 34 110 1.21e-13 65.1
MS.gene96804.t1 MTR_3g084040 38.750 80 47 2 33 112 22 99 6.67e-13 62.4
MS.gene96804.t1 MTR_8g031560 33.607 122 71 3 16 128 159 279 2.05e-12 64.3
MS.gene96804.t1 MTR_3g080130 33.913 115 72 3 30 141 18 131 9.61e-12 60.1
MS.gene96804.t1 MTR_4g070190 35.526 76 49 0 41 116 1 76 1.05e-11 60.5
MS.gene96804.t1 MTR_2g096560 32.099 81 55 0 50 130 9 89 1.25e-11 58.5
MS.gene96804.t1 MTR_8g107310 37.662 77 48 0 52 128 215 291 3.07e-11 60.8
MS.gene96804.t1 MTR_4g070080 37.879 66 41 0 51 116 9 74 4.93e-11 58.2
MS.gene96804.t1 MTR_4g070140 40.909 66 39 0 51 116 11 76 8.28e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96804.t1 AT5G54580 56.618 136 55 1 6 141 18 149 1.20e-46 150
MS.gene96804.t1 AT2G37510 47.706 109 55 1 50 158 36 142 2.66e-23 90.5
MS.gene96804.t1 AT1G73530 45.238 84 46 0 46 129 75 158 2.57e-21 86.3
MS.gene96804.t1 AT2G37510 38.806 134 55 2 50 158 36 167 1.61e-17 76.3
MS.gene96804.t1 AT5G61030 45.570 79 43 0 50 128 42 120 8.77e-17 76.6
MS.gene96804.t1 AT5G61030 45.570 79 43 0 50 128 42 120 8.77e-17 76.6
MS.gene96804.t1 AT5G06210 43.590 78 44 0 50 127 36 113 9.75e-17 73.6
MS.gene96804.t1 AT3G46020 38.158 76 47 0 50 125 9 84 2.88e-16 71.2
MS.gene96804.t1 AT3G20930 37.179 78 49 0 50 127 284 361 1.51e-15 73.9
MS.gene96804.t1 AT3G23830 43.210 81 46 0 50 130 37 117 3.52e-15 69.3
MS.gene96804.t1 AT3G23830 43.210 81 46 0 50 130 37 117 3.52e-15 69.3
MS.gene96804.t1 AT1G18630 47.143 70 37 0 50 119 38 107 1.23e-13 65.5
MS.gene96804.t1 AT3G20930 34.940 83 49 1 50 127 284 366 1.95e-13 67.8
MS.gene96804.t1 AT4G13850 36.111 108 55 2 16 116 3 103 2.82e-13 63.9
MS.gene96804.t1 AT4G13850 36.111 108 55 2 16 116 3 103 4.53e-13 63.9
MS.gene96804.t1 AT4G13850 36.111 108 55 2 16 116 3 103 5.44e-13 63.9
MS.gene96804.t1 AT4G13850 36.111 108 55 2 16 116 3 103 6.01e-13 63.9
MS.gene96804.t1 AT5G59865 41.026 78 46 0 50 127 28 105 1.86e-12 61.6
MS.gene96804.t1 AT1G74230 35.135 111 62 3 14 124 9 109 4.22e-12 63.5
MS.gene96804.t1 AT4G13860 43.284 67 38 0 50 116 5 71 5.21e-12 59.7
MS.gene96804.t1 AT3G08000 43.284 67 38 0 50 116 43 109 5.21e-12 60.8
MS.gene96804.t1 AT2G43370 32.530 83 56 0 43 125 59 141 2.79e-11 61.6
MS.gene96804.t1 AT2G27330 32.877 73 49 0 50 122 23 95 2.82e-11 58.5
MS.gene96804.t1 AT5G19030 30.263 76 53 0 51 126 80 155 2.87e-11 59.7
MS.gene96804.t1 AT2G43370 32.530 83 56 0 43 125 59 141 5.52e-11 59.3
MS.gene96804.t1 AT5G47320 30.088 113 69 2 16 125 3 108 8.46e-11 58.9

Find 52 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACCGGTGACGAAGAGAATT+TGG 0.272558 4.3:-28571514 None:intergenic
GAGGCTATAGCATTGGTTCT+TGG 0.297566 4.3:-28573324 None:intergenic
AAAGTTCGGTGAAGTTAAAC+AGG 0.321973 4.3:+28572386 MS.gene96804:CDS
TCAGGTTACTCTAAGGGATT+TGG 0.331272 4.3:+28572565 MS.gene96804:CDS
TCGTGTCTCAGGTTACTCTA+AGG 0.355649 4.3:+28572558 MS.gene96804:CDS
TTCATTTCACCGTTCTTCTC+TGG 0.361360 4.3:-28573399 None:intergenic
GGGATGGTTCAGTATGTGTC+TGG 0.374916 4.3:-28573347 None:intergenic
CGGTGCCCCGCGGTTTCTGC+CGG 0.374929 4.3:+28571393 MS.gene96804:CDS
TTATCCTAATCTTTGTCCAT+TGG 0.396575 4.3:-28573486 None:intergenic
GAAAAGCCGGCAGAAACCGC+GGG 0.416020 4.3:-28571399 None:intergenic
CGAACCAATGGACAAAGATT+AGG 0.427675 4.3:+28573482 MS.gene96804:CDS
GATGCAGCTAAGGGCATTCA+AGG 0.474539 4.3:+28572613 MS.gene96804:CDS
AGAGCAGAAGTAGCTCTAGA+AGG 0.482168 4.3:-28571473 None:intergenic
TACTTCAGAAGATGCAGCTA+AGG 0.490832 4.3:+28572603 MS.gene96804:CDS
GTGGTGGCAGATCGTGTCTC+AGG 0.492116 4.3:+28572547 MS.gene96804:CDS
AAGGCGGCGTTGAAGCTAAC+GGG 0.496720 4.3:-28571454 None:intergenic
GATGAGAGCTACAGCGGCAG+CGG 0.497516 4.3:+28571373 None:intergenic
TTGGCTTCGTGCAGAAAAGC+CGG 0.502812 4.3:-28571412 None:intergenic
GTGACGAAGAGAATTTGGTT+GGG 0.517626 4.3:-28571509 None:intergenic
AACCAAATTCTCTTCGTCAC+CGG 0.521059 4.3:+28571512 MS.gene96804:CDS
TCTTCAGGTTCGGAGTGGGA+TGG 0.524520 4.3:-28573363 None:intergenic
GCCGATCTCAGCAAGGGTGG+TGG 0.532889 4.3:+28572531 MS.gene96804:intron
ACAGCGGCAGCGGTGCCCCG+CGG 0.533814 4.3:+28571383 MS.gene96804:CDS
GGTGACGAAGAGAATTTGGT+TGG 0.541116 4.3:-28571510 None:intergenic
CGTGTCTCAGGTTACTCTAA+GGG 0.541129 4.3:+28572559 MS.gene96804:CDS
GAAGAACGGTGAAATGAAGA+AGG 0.549581 4.3:+28573404 MS.gene96804:CDS
GGATGGTTCAGTATGTGTCT+GGG 0.554245 4.3:-28573346 None:intergenic
AAAACGACGCCAGAGAAGAA+CGG 0.554308 4.3:+28573390 MS.gene96804:CDS
GAAGGCGGCGTTGAAGCTAA+CGG 0.555224 4.3:-28571455 None:intergenic
GAAGGAAACTGCTGGCAAGA+AGG 0.563083 4.3:+28573422 MS.gene96804:CDS
TGTCTGGGAGGCTATAGCAT+TGG 0.576971 4.3:-28573331 None:intergenic
CATTCAAGGCATGGATGGCA+AGG 0.577087 4.3:+28572627 MS.gene96804:CDS
AAGGGCATTCAAGGCATGGA+TGG 0.580521 4.3:+28572622 MS.gene96804:CDS
AGAAAAGCCGGCAGAAACCG+CGG 0.588918 4.3:-28571400 None:intergenic
AGCTAAGGGCATTCAAGGCA+TGG 0.591683 4.3:+28572618 MS.gene96804:CDS
ACTTCAGAAGATGCAGCTAA+GGG 0.597665 4.3:+28572604 MS.gene96804:CDS
AGCTACTTCTGCTCTAAAGA+CGG 0.598572 4.3:+28571481 MS.gene96804:CDS
GGTAGTTGCAGACGAACCAA+TGG 0.606038 4.3:+28573470 MS.gene96804:CDS
GTTGAAGCTAACGGGACAGT+GGG 0.607756 4.3:-28571446 None:intergenic
TTTGCCGATCTCAGCAAGGG+TGG 0.614124 4.3:+28572528 MS.gene96804:intron
CGTTGAAGCTAACGGGACAG+TGG 0.621233 4.3:-28571447 None:intergenic
CGGGACAGTGGGAGAAGACG+CGG 0.638265 4.3:-28571435 None:intergenic
GAAATGAAGAAGGAAACTGC+TGG 0.640066 4.3:+28573414 MS.gene96804:CDS
TGGTTCAGTATGTGTCTGGG+AGG 0.643151 4.3:-28573343 None:intergenic
AAAAGCCGGCAGAAACCGCG+GGG 0.647049 4.3:-28571398 None:intergenic
ACAGTGGGAGAAGACGCGGT+TGG 0.653547 4.3:-28571431 None:intergenic
GTACTACATCAGAGACGCTG+AGG 0.664662 4.3:+28572352 MS.gene96804:CDS
TTTGATGATGAGAGCTACAG+CGG 0.670929 4.3:+28571367 None:intergenic
TACTACATCAGAGACGCTGA+GGG 0.671573 4.3:+28572353 MS.gene96804:CDS
TACTTCTGCTCTAAAGACGG+CGG 0.680130 4.3:+28571484 MS.gene96804:CDS
GCAGAAGTAGCTCTAGAAGG+CGG 0.685533 4.3:-28571470 None:intergenic
AAGTTCGGTGAAGTTAAACA+GGG 0.706995 4.3:+28572387 MS.gene96804:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAATAATTGAATCGAAT+CGG - chr4.3:28573238-28573257 None:intergenic 20.0%
!! CAAATGTTAAAATTTACCAA+GGG - chr4.3:28572425-28572444 None:intergenic 20.0%
!! TCAAATGTTAAAATTTACCA+AGG - chr4.3:28572426-28572445 None:intergenic 20.0%
!! TCATAATATTCATTTGATTG+TGG + chr4.3:28572293-28572312 MS.gene96804:intron 20.0%
!! TGTTAATGTAAACTGTAAAT+TGG + chr4.3:28571564-28571583 MS.gene96804:intron 20.0%
!! TTATGAAAGACAAATTGAAT+TGG - chr4.3:28572279-28572298 None:intergenic 20.0%
!! TTTATGCAATTTGTAACAAA+AGG - chr4.3:28571701-28571720 None:intergenic 20.0%
!!! ATGATTTTTATGTTCAACAA+AGG + chr4.3:28571725-28571744 MS.gene96804:intron 20.0%
!!! GACAACTTCATTTTTATTTA+TGG - chr4.3:28572229-28572248 None:intergenic 20.0%
! AATCATAGCTGAAACTATAA+TGG + chr4.3:28572498-28572517 MS.gene96804:intron 25.0%
! CAAAACACATAATTACATTC+CGG - chr4.3:28573105-28573124 None:intergenic 25.0%
! TAAACAAAGTTTATCTTAGG+TGG - chr4.3:28572783-28572802 None:intergenic 25.0%
! TAAATGTGTCATAACTTTCA+GGG + chr4.3:28572319-28572338 MS.gene96804:intron 25.0%
! TGTTTAAAGAGGGAAATTAA+GGG - chr4.3:28572155-28572174 None:intergenic 25.0%
! TTAAATGTGTCATAACTTTC+AGG + chr4.3:28572318-28572337 MS.gene96804:intron 25.0%
!! CTCTTTTCTTTTTCTTATCA+TGG - chr4.3:28571858-28571877 None:intergenic 25.0%
!! TGTTAGTATTTTTCTCTTGT+CGG - chr4.3:28571981-28572000 None:intergenic 25.0%
!!! ACTGATTTGAGTTTATTACT+TGG + chr4.3:28571778-28571797 MS.gene96804:intron 25.0%
!!! ACTTCATTTTTATTTATGGC+AGG - chr4.3:28572225-28572244 None:intergenic 25.0%
!!! CTTCATTTTTATTTATGGCA+GGG - chr4.3:28572224-28572243 None:intergenic 25.0%
!!! GTTCTCATTTGATTTGATTT+AGG + chr4.3:28571659-28571678 MS.gene96804:intron 25.0%
!!! TTCATTTTTATTTATGGCAG+GGG - chr4.3:28572223-28572242 None:intergenic 25.0%
AAGAATAGTGAATGCATTGT+TGG - chr4.3:28572865-28572884 None:intergenic 30.0%
AATGACAGTTGTAACATGAA+TGG - chr4.3:28572180-28572199 None:intergenic 30.0%
AATGCATGATTTACAGATTC+AGG - chr4.3:28573040-28573059 None:intergenic 30.0%
AGATCATTGAATTTCATGTC+AGG - chr4.3:28572983-28573002 None:intergenic 30.0%
AGTAATAAGCAATTACTGAC+CGG - chr4.3:28571534-28571553 None:intergenic 30.0%
AGTAATCTCTCAAACGATTT+GGG + chr4.3:28571883-28571902 MS.gene96804:intron 30.0%
ATGAATGGTGTGTTTAAAGA+GGG - chr4.3:28572165-28572184 None:intergenic 30.0%
ATGCATGATTTACAGATTCA+GGG - chr4.3:28573039-28573058 None:intergenic 30.0%
CGGTAAACAAAGTTTATCTT+AGG - chr4.3:28572786-28572805 None:intergenic 30.0%
GAAAAAGAAGATAACTGCAA+AGG + chr4.3:28573449-28573468 MS.gene96804:CDS 30.0%
GTGTTTAAAGAGGGAAATTA+AGG - chr4.3:28572156-28572175 None:intergenic 30.0%
GTTTAAAGAGGGAAATTAAG+GGG - chr4.3:28572154-28572173 None:intergenic 30.0%
TAGTAATCTCTCAAACGATT+TGG + chr4.3:28571882-28571901 MS.gene96804:intron 30.0%
TCTTAAAGCACGAATTTGTA+TGG + chr4.3:28572881-28572900 MS.gene96804:intron 30.0%
TTTCTTATCATGGCAAATGT+TGG - chr4.3:28571848-28571867 None:intergenic 30.0%
! AGTTGTCTATTGCATTGATA+AGG + chr4.3:28572242-28572261 MS.gene96804:intron 30.0%
! GTTAGTATTTTTCTCTTGTC+GGG - chr4.3:28571980-28571999 None:intergenic 30.0%
! TTCTAAAAACTGCATTGCAA+AGG - chr4.3:28573274-28573293 None:intergenic 30.0%
!! ACATTGTTGTTGTTTATGTC+TGG + chr4.3:28573143-28573162 MS.gene96804:intron 30.0%
!! AGGGAAGCATTTAAAAAGTT+CGG + chr4.3:28572372-28572391 MS.gene96804:CDS 30.0%
!! TGATTTAGGTTGTCTTGTTA+CGG + chr4.3:28571673-28571692 MS.gene96804:intron 30.0%
!! TTTGCAATGCAGTTTTTAGA+AGG + chr4.3:28573273-28573292 MS.gene96804:intron 30.0%
!!! TCGTATTTCATGTTTTGATC+AGG + chr4.3:28571610-28571629 MS.gene96804:intron 30.0%
AAAGGATCATTTCTTCTTCG+AGG + chr4.3:28571743-28571762 MS.gene96804:intron 35.0%
AAATTAAGGGGAATGCTGTT+TGG - chr4.3:28572142-28572161 None:intergenic 35.0%
AGAAAAGCAAAGAACAACCA+TGG - chr4.3:28573205-28573224 None:intergenic 35.0%
AGTGTGATGTGTATATGTTG+CGG - chr4.3:28572812-28572831 None:intergenic 35.0%
CATGAATGGTGTGTTTAAAG+AGG - chr4.3:28572166-28572185 None:intergenic 35.0%
CTAAGGGATTAAAGAAGTCA+AGG + chr4.3:28571931-28571950 MS.gene96804:intron 35.0%
CTGTCAGAATATTGTTGTCT+TGG + chr4.3:28572709-28572728 MS.gene96804:intron 35.0%
GGTTGAGATCAAAATAGCTA+GGG - chr4.3:28572095-28572114 None:intergenic 35.0%
GTAATCTCTCAAACGATTTG+GGG + chr4.3:28571884-28571903 MS.gene96804:intron 35.0%
TAAATAGCTGAACGAGTGAA+TGG - chr4.3:28572450-28572469 None:intergenic 35.0%
TCATTGAATTTCATGTCAGG+AGG - chr4.3:28572980-28572999 None:intergenic 35.0%
TTTACTGCAAACTCGAATAG+AGG - chr4.3:28572908-28572927 None:intergenic 35.0%
! AAAGTTCGGTGAAGTTAAAC+AGG + chr4.3:28572386-28572405 MS.gene96804:CDS 35.0%
! AAGTTCGGTGAAGTTAAACA+GGG + chr4.3:28572387-28572406 MS.gene96804:CDS 35.0%
! GCATCTTTTTACATGACAAC+CGG + chr4.3:28573083-28573102 MS.gene96804:intron 35.0%
! TAATCTCTCAAACGATTTGG+GGG + chr4.3:28571885-28571904 MS.gene96804:intron 35.0%
!! ACAACTCACTCTAGTCTTTT+GGG - chr4.3:28572047-28572066 None:intergenic 35.0%
!! CGTTTTCTTTTCTTCAGGTT+CGG - chr4.3:28573376-28573395 None:intergenic 35.0%
!! GATTTAGGTTGTCTTGTTAC+GGG + chr4.3:28571674-28571693 MS.gene96804:intron 35.0%
!!! ATGTTTTGATCAGGTTGTTG+AGG + chr4.3:28571619-28571638 MS.gene96804:intron 35.0%
AACCAAATTCTCTTCGTCAC+CGG + chr4.3:28571512-28571531 MS.gene96804:CDS 40.0%
AATTTCATGTCAGGAGGAAG+GGG - chr4.3:28572974-28572993 None:intergenic 40.0%
ACTTCAGAAGATGCAGCTAA+GGG + chr4.3:28572604-28572623 MS.gene96804:CDS 40.0%
ACTTGGTTCAATCTCGACAA+AGG + chr4.3:28571795-28571814 MS.gene96804:intron 40.0%
ATCTCAACCCACAATTTGCA+TGG + chr4.3:28572106-28572125 MS.gene96804:intron 40.0%
ATGTGTATATGTTGCGGTGT+CGG - chr4.3:28572806-28572825 None:intergenic 40.0%
CAGTATTGTGTTACATGTGC+AGG - chr4.3:28572679-28572698 None:intergenic 40.0%
GAAATGAAGAAGGAAACTGC+TGG + chr4.3:28573414-28573433 MS.gene96804:CDS 40.0%
GAAGAACGGTGAAATGAAGA+AGG + chr4.3:28573404-28573423 MS.gene96804:CDS 40.0%
GAATTTCATGTCAGGAGGAA+GGG - chr4.3:28572975-28572994 None:intergenic 40.0%
GGGTTGAGATCAAAATAGCT+AGG - chr4.3:28572096-28572115 None:intergenic 40.0%
GTGACGAAGAGAATTTGGTT+GGG - chr4.3:28571512-28571531 None:intergenic 40.0%
TACTTCAGAAGATGCAGCTA+AGG + chr4.3:28572603-28572622 MS.gene96804:CDS 40.0%
TGAATTTCATGTCAGGAGGA+AGG - chr4.3:28572976-28572995 None:intergenic 40.0%
TTCATTTCACCGTTCTTCTC+TGG - chr4.3:28573402-28573421 None:intergenic 40.0%
TTTATTTATGGCAGGGGAAG+AGG - chr4.3:28572217-28572236 None:intergenic 40.0%
! TCAGGTTACTCTAAGGGATT+TGG + chr4.3:28572565-28572584 MS.gene96804:CDS 40.0%
!! AGCTACTTCTGCTCTAAAGA+CGG + chr4.3:28571481-28571500 MS.gene96804:CDS 40.0%
!! CAATGCAGTTTTTAGAAGGC+TGG + chr4.3:28573277-28573296 MS.gene96804:intron 40.0%
!! CACAACTCACTCTAGTCTTT+TGG - chr4.3:28572048-28572067 None:intergenic 40.0%
!! CATCTACAAAGTTGATGCCA+TGG + chr4.3:28573185-28573204 MS.gene96804:intron 40.0%
AAAACGACGCCAGAGAAGAA+CGG + chr4.3:28573390-28573409 MS.gene96804:CDS 45.0%
AACCCACAATTTGCATGGTG+TGG + chr4.3:28572111-28572130 MS.gene96804:intron 45.0%
AGGGTATGTTCTTCATCCCT+TGG + chr4.3:28572406-28572425 MS.gene96804:intron 45.0%
AGTCAAGGAGTTCAAGATCC+AGG + chr4.3:28571946-28571965 MS.gene96804:intron 45.0%
ATTGTGTTACATGTGCAGGC+AGG - chr4.3:28572675-28572694 None:intergenic 45.0%
ATTTCATGTCAGGAGGAAGG+GGG - chr4.3:28572973-28572992 None:intergenic 45.0%
CACCACACCATGCAAATTGT+GGG - chr4.3:28572116-28572135 None:intergenic 45.0%
CGTGTCTCAGGTTACTCTAA+GGG + chr4.3:28572559-28572578 MS.gene96804:CDS 45.0%
GGATGGTTCAGTATGTGTCT+GGG - chr4.3:28573349-28573368 None:intergenic 45.0%
GGTGACGAAGAGAATTTGGT+TGG - chr4.3:28571513-28571532 None:intergenic 45.0%
GTCAAGGAGTTCAAGATCCA+GGG + chr4.3:28571947-28571966 MS.gene96804:intron 45.0%
TACTACATCAGAGACGCTGA+GGG + chr4.3:28572353-28572372 MS.gene96804:CDS 45.0%
TCACCACACCATGCAAATTG+TGG - chr4.3:28572117-28572136 None:intergenic 45.0%
TCGTGTCTCAGGTTACTCTA+AGG + chr4.3:28572558-28572577 MS.gene96804:CDS 45.0%
! TCTCAAACGATTTGGGGGTT+TGG + chr4.3:28571890-28571909 MS.gene96804:intron 45.0%
!! AGAGCAGAAGTAGCTCTAGA+AGG - chr4.3:28571476-28571495 None:intergenic 45.0%
!! AGCTCAACTGGTTTGAGCTA+AGG + chr4.3:28571914-28571933 MS.gene96804:intron 45.0%
!! CTTTTCTTCAGGTTCGGAGT+GGG - chr4.3:28573370-28573389 None:intergenic 45.0%
!! GAGGCTATAGCATTGGTTCT+TGG - chr4.3:28573327-28573346 None:intergenic 45.0%
!! GCTCAACTGGTTTGAGCTAA+GGG + chr4.3:28571915-28571934 MS.gene96804:intron 45.0%
!! GGCGTCGTTTTCTTTTCTTC+AGG - chr4.3:28573381-28573400 None:intergenic 45.0%
!! TACTTCTGCTCTAAAGACGG+CGG + chr4.3:28571484-28571503 MS.gene96804:CDS 45.0%
!! TCTTTTCTTCAGGTTCGGAG+TGG - chr4.3:28573371-28573390 None:intergenic 45.0%
ACATGTGCAGGCAGGCTAAA+GGG - chr4.3:28572667-28572686 None:intergenic 50.0%
CATTCAAGGCATGGATGGCA+AGG + chr4.3:28572627-28572646 MS.gene96804:CDS 50.0%
GAAGGAAACTGCTGGCAAGA+AGG + chr4.3:28573422-28573441 MS.gene96804:CDS 50.0%
GACCGGTGACGAAGAGAATT+TGG - chr4.3:28571517-28571536 None:intergenic 50.0%
GGGATGGTTCAGTATGTGTC+TGG - chr4.3:28573350-28573369 None:intergenic 50.0%
GGTAGTTGCAGACGAACCAA+TGG + chr4.3:28573470-28573489 MS.gene96804:CDS 50.0%
GTACTACATCAGAGACGCTG+AGG + chr4.3:28572352-28572371 MS.gene96804:CDS 50.0%
GTTGAAGCTAACGGGACAGT+GGG - chr4.3:28571449-28571468 None:intergenic 50.0%
TACATGTGCAGGCAGGCTAA+AGG - chr4.3:28572668-28572687 None:intergenic 50.0%
TGGTTCAGTATGTGTCTGGG+AGG - chr4.3:28573346-28573365 None:intergenic 50.0%
TGTCTGGGAGGCTATAGCAT+TGG - chr4.3:28573334-28573353 None:intergenic 50.0%
TTGGCTTCGTGCAGAAAAGC+CGG - chr4.3:28571415-28571434 None:intergenic 50.0%
TTTCATGTCAGGAGGAAGGG+GGG - chr4.3:28572972-28572991 None:intergenic 50.0%
!! AAGGGCATTCAAGGCATGGA+TGG + chr4.3:28572622-28572641 MS.gene96804:CDS 50.0%
!! AGCTAAGGGCATTCAAGGCA+TGG + chr4.3:28572618-28572637 MS.gene96804:CDS 50.0%
!! GATGCAGCTAAGGGCATTCA+AGG + chr4.3:28572613-28572632 MS.gene96804:CDS 50.0%
!! GCAGAAGTAGCTCTAGAAGG+CGG - chr4.3:28571473-28571492 None:intergenic 50.0%
AGAAAAGCCGGCAGAAACCG+CGG - chr4.3:28571403-28571422 None:intergenic 55.0%
CCCTTGCTGAGATCGGCAAA+CGG - chr4.3:28572528-28572547 None:intergenic 55.0%
CCGTTTGCCGATCTCAGCAA+GGG + chr4.3:28572525-28572544 MS.gene96804:intron 55.0%
CGTTGAAGCTAACGGGACAG+TGG - chr4.3:28571450-28571469 None:intergenic 55.0%
TCCGTTTGCCGATCTCAGCA+AGG + chr4.3:28572524-28572543 MS.gene96804:intron 55.0%
TCTTGTCGGGACTTGAACCC+TGG - chr4.3:28571967-28571986 None:intergenic 55.0%
TTCATGTCAGGAGGAAGGGG+GGG - chr4.3:28572971-28572990 None:intergenic 55.0%
TTTGCCGATCTCAGCAAGGG+TGG + chr4.3:28572528-28572547 MS.gene96804:intron 55.0%
! AAGGCGGCGTTGAAGCTAAC+GGG - chr4.3:28571457-28571476 None:intergenic 55.0%
! GAAGGCGGCGTTGAAGCTAA+CGG - chr4.3:28571458-28571477 None:intergenic 55.0%
! TCTTCAGGTTCGGAGTGGGA+TGG - chr4.3:28573366-28573385 None:intergenic 55.0%
! TGGGGGTTTGGTAGCTCAAC+TGG + chr4.3:28571902-28571921 MS.gene96804:intron 55.0%
AAAAGCCGGCAGAAACCGCG+GGG - chr4.3:28571401-28571420 None:intergenic 60.0%
ACAGTGGGAGAAGACGCGGT+TGG - chr4.3:28571434-28571453 None:intergenic 60.0%
GAAAAGCCGGCAGAAACCGC+GGG - chr4.3:28571402-28571421 None:intergenic 60.0%
GCCACCACCCTTGCTGAGAT+CGG - chr4.3:28572535-28572554 None:intergenic 60.0%
GTGGTGGCAGATCGTGTCTC+AGG + chr4.3:28572547-28572566 MS.gene96804:CDS 60.0%
TCATGTCAGGAGGAAGGGGG+GGG - chr4.3:28572970-28572989 None:intergenic 60.0%
CGGGACAGTGGGAGAAGACG+CGG - chr4.3:28571438-28571457 None:intergenic 65.0%
GCCGATCTCAGCAAGGGTGG+TGG + chr4.3:28572531-28572550 MS.gene96804:intron 65.0%
CGGTGCCCCGCGGTTTCTGC+CGG + chr4.3:28571393-28571412 MS.gene96804:CDS 75.0%
! ACAGCGGCAGCGGTGCCCCG+CGG + chr4.3:28571383-28571402 MS.gene96804:CDS 80.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 28571374 28573503 28571374 ID=MS.gene96804
chr4.3 mRNA 28571374 28573503 28571374 ID=MS.gene96804.t1;Parent=MS.gene96804
chr4.3 exon 28571374 28571533 28571374 ID=MS.gene96804.t1.exon1;Parent=MS.gene96804.t1
chr4.3 CDS 28571374 28571533 28571374 ID=cds.MS.gene96804.t1;Parent=MS.gene96804.t1
chr4.3 exon 28572340 28572408 28572340 ID=MS.gene96804.t1.exon2;Parent=MS.gene96804.t1
chr4.3 CDS 28572340 28572408 28572340 ID=cds.MS.gene96804.t1;Parent=MS.gene96804.t1
chr4.3 exon 28572542 28572648 28572542 ID=MS.gene96804.t1.exon3;Parent=MS.gene96804.t1
chr4.3 CDS 28572542 28572648 28572542 ID=cds.MS.gene96804.t1;Parent=MS.gene96804.t1
chr4.3 exon 28573285 28573503 28573285 ID=MS.gene96804.t1.exon4;Parent=MS.gene96804.t1
chr4.3 CDS 28573285 28573503 28573285 ID=cds.MS.gene96804.t1;Parent=MS.gene96804.t1
Gene Sequence

>MS.gene96804

ATGAGAGCTACAGCGGCAGCGGTGCCCCGCGGTTTCTGCCGGCTTTTCTGCACGAAGCCAACCGCGTCTTCTCCCACTGTCCCGTTAGCTTCAACGCCGCCTTCTAGAGCTACTTCTGCTCTAAAGACGGCGGAGCCCAACCAAATTCTCTTCGTCACCGGTCAGTAATTGCTTATTACTATCTATCTATTGTTAATGTAAACTGTAAATTGGAGTGTATGTTTTGATATTGTTGTTCGTATTTCATGTTTTGATCAGGTTGTTGAGGTTTTATTTCATGTATATGTTCTCATTTGATTTGATTTAGGTTGTCTTGTTACGGGTCCTTTTGTTACAAATTGCATAAAAAAAATGATTTTTATGTTCAACAAAGGATCATTTCTTCTTCGAGGCTGAGTAGTTCAACTGATTTGAGTTTATTACTTGGTTCAATCTCGACAAAGGAGTAAATATTAATCGAATAACTAATTACCAACATTTGCCATGATAAGAAAAAGAAAAGAGTAAATAGTAATCTCTCAAACGATTTGGGGGTTTGGTAGCTCAACTGGTTTGAGCTAAGGGATTAAAGAAGTCAAGGAGTTCAAGATCCAGGGTTCAAGTCCCGACAAGAGAAAAATACTAACATAACAATTAACATACTCACATTTTTCATTCAAAAAAGAAATCTCCCAAAAGACTAGAGTGAGTTGTGCATTGTTTGATTGCAGTGTTAACTCCCTAGCTATTTTGATCTCAACCCACAATTTGCATGGTGTGGTGACGCCAAACAGCATTCCCCTTAATTTCCCTCTTTAAACACACCATTCATGTTACAACTGTCATTTTCAATTTTTATCTCCTCTTCCCCTGCCATAAATAAAAATGAAGTTGTCTATTGCATTGATAAGGTCATTCTTTGTCCAATTCAATTTGTCTTTCATAATATTCATTTGATTGTGGTTTTAAATGTGTCATAACTTTCAGGGCTTAACAAACGTACTACATCAGAGACGCTGAGGGAAGCATTTAAAAAGTTCGGTGAAGTTAAACAGGGTATGTTCTTCATCCCTTGGTAAATTTTAACATTTGATCCATTCACTCGTTCAGCTATTTATGCTTTCACTATGCGATGATGTCTTAAAAATCATAGCTGAAACTATAATGGTTTTCCGTTTGCCGATCTCAGCAAGGGTGGTGGCAGATCGTGTCTCAGGTTACTCTAAGGGATTTGGTTACGTGAAATATGCTACTTCAGAAGATGCAGCTAAGGGCATTCAAGGCATGGATGGCAAGGTCAAATTTATTAAACCCTTTAGCCTGCCTGCACATGTAACACAATACTGAAATGTATTTCTGTCAGAATATTGTTGTCTTGGATTTGTCTCTGCATTTGTTTATTTATGCAAAATAAAAATTTAATATGACCACCTAAGATAAACTTTGTTTACCGACACCGCAACATATACACATCACACTAGAAGACTGAACTCTAATTGCTTGACATTGCCAACAATGCATTCACTATTCTTAAAGCACGAATTTGTATGGACCTCTATTCGAGTTTGCAGTAAATCTCATTTACTATTATTTGAACTCAAGTTTTCTCAATTTCCCCCCCCTTCCTCCTGACATGAAATTCAATGATCTAACTCTTCAATATTTAGAGTATTTATTTAATCACCCTGAATCTGTAAATCATGCATTGATCAGTTAGCTCAAATTAATATGCATCTTTTTACATGACAACCGGAATGTAATTATGTGTTTTGTTTTCATGAATTTTCGTGACATTGTTGTTGTTTATGTCTGGAAAACTTTTTTGTGTAGTTCATCTACAAAGTTGATGCCATGGTTGTTCTTTGCTTTTCTTACAAAATTTCCGATTCGATTCAATTATTTCTTACTGATATGTTTTCCTTTGCAATGCAGTTTTTAGAAGGCTGGATTGTATTTGCAGAGTATGCAAGACCAAGAACCAATGCTATAGCCTCCCAGACACATACTGAACCATCCCACTCCGAACCTGAAGAAAAGAAAACGACGCCAGAGAAGAACGGTGAAATGAAGAAGGAAACTGCTGGCAAGAAGGACAAGAAAAAGAAGATAACTGCAAAGGTAGTTGCAGACGAACCAATGGACAAAGATTAG
Protein sequence

>MS.gene96804.t1

MRATAAAVPRGFCRLFCTKPTASSPTVPLASTPPSRATSALKTAEPNQILFVTGLNKRTTSETLREAFKKFGEVKQARVVADRVSGYSKGFGYVKYATSEDAAKGIQGMDGKFLEGWIVFAEYARPRTNAIASQTHTEPSHSEPEEKKTTPEKNGEMKKETAGKKDKKKKITAKVVADEPMDKD