Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97209.t1 | XP_003621422.1 | 98.8 | 251 | 3 | 0 | 1 | 251 | 5 | 255 | 8.80E-143 | 516.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97209.t1 | Q9FL82 | 66.1 | 254 | 81 | 3 | 1 | 251 | 1 | 252 | 5.3e-96 | 352.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97209.t1 | B7FID8 | 98.8 | 251 | 3 | 0 | 1 | 251 | 1 | 251 | 6.3e-143 | 516.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057478 | MS.gene97209 | 0.963956 | 1.01E-122 | -1.69E-46 |
| MS.gene060818 | MS.gene97209 | -0.841711 | 4.03E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97209.t1 | MTR_7g013160 | 98.805 | 251 | 3 | 0 | 1 | 251 | 5 | 255 | 0.0 | 513 |
| MS.gene97209.t1 | MTR_7g013160 | 98.805 | 251 | 3 | 0 | 1 | 251 | 1 | 251 | 0.0 | 513 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97209.t1 | AT5G39250 | 66.142 | 254 | 81 | 3 | 1 | 251 | 1 | 252 | 3.40e-122 | 348 |
| MS.gene97209.t1 | AT5G39250 | 66.142 | 254 | 81 | 3 | 1 | 251 | 1 | 252 | 3.40e-122 | 348 |
Find 56 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTACGTTGTTGGCGTTTA+TGG | 0.113675 | 7.3:-84598137 | None:intergenic |
| GAAGATCTTATGGATGTATT+TGG | 0.156608 | 7.3:+84598588 | MS.gene97209:CDS |
| TGCCCGGATGCCGGGCAAAA+AGG | 0.191357 | 7.3:-84598532 | None:intergenic |
| GAGAATTTCCTTTGATGATT+TGG | 0.254747 | 7.3:+84598173 | MS.gene97209:CDS |
| TAGGTTTCAGTAGGAGGATT+AGG | 0.276690 | 7.3:-84598093 | None:intergenic |
| TAGCTAAAGACGATTTCTTT+TGG | 0.285765 | 7.3:+84598028 | MS.gene97209:CDS |
| CTGTGATGTGGCAAATTCTA+AGG | 0.293619 | 7.3:+84598626 | MS.gene97209:CDS |
| AGAGGGAAGTGAAGATCTTA+TGG | 0.307374 | 7.3:+84598578 | MS.gene97209:CDS |
| ATGGATATCTTTGCTATTCA+TGG | 0.339435 | 7.3:+84598554 | MS.gene97209:CDS |
| CTCTTCTCGGAAGCGGTGTC+TGG | 0.348746 | 7.3:+84598231 | MS.gene97209:CDS |
| GCAGATTGATGGCCATCTAT+TGG | 0.358781 | 7.3:-84598064 | None:intergenic |
| TCTGGCTCTGTCCTGCAATC+AGG | 0.360560 | 7.3:+84598249 | MS.gene97209:CDS |
| GGAAGAAGTCTTGTGGCTAT+TGG | 0.366589 | 7.3:+84598647 | MS.gene97209:CDS |
| CCACATCACAGAAATCCATT+TGG | 0.372208 | 7.3:-84598614 | None:intergenic |
| TGTTGATCTTGCTTCTTGTA+TGG | 0.375347 | 7.3:+84597981 | MS.gene97209:CDS |
| AAGATCTTATGGATGTATTT+GGG | 0.377784 | 7.3:+84598589 | MS.gene97209:CDS |
| GACTTCTGAAACCTGATTGC+AGG | 0.382994 | 7.3:-84598260 | None:intergenic |
| GGTTTCAGAAGTCCAGCTTC+TGG | 0.386300 | 7.3:+84598270 | MS.gene97209:CDS |
| TGGGCGGAAGGATACAAATA+AGG | 0.418384 | 7.3:+84598413 | MS.gene97209:CDS |
| TTAGGTCGCTTGCAGATTGA+TGG | 0.425972 | 7.3:-84598075 | None:intergenic |
| GGCTATTGGACATGCTAGAT+TGG | 0.433276 | 7.3:+84598661 | MS.gene97209:CDS |
| AGATATCCATGCCCGGATGC+CGG | 0.435632 | 7.3:-84598541 | None:intergenic |
| GGAGAAGAGTTCTACGTTGT+TGG | 0.464005 | 7.3:-84598146 | None:intergenic |
| TGAAGATATACTTCTCTTCT+CGG | 0.464021 | 7.3:+84598218 | MS.gene97209:CDS |
| AAATCATCAAAGGAAATTCT+CGG | 0.464319 | 7.3:-84598171 | None:intergenic |
| ATCTTTGCTATTCATGGAAG+AGG | 0.467936 | 7.3:+84598560 | MS.gene97209:CDS |
| GATCAACACCCTCCAGTAAA+GGG | 0.470826 | 7.3:-84597966 | None:intergenic |
| GATATCCATGCCCGGATGCC+GGG | 0.472969 | 7.3:-84598540 | None:intergenic |
| GGATGTATTTGGGATCCAAA+TGG | 0.475681 | 7.3:+84598599 | MS.gene97209:CDS |
| ATAGTAAACCTAGGTTCAAC+CGG | 0.490159 | 7.3:-84598348 | None:intergenic |
| TTGTAGTAGGTTTCAGTAGG+AGG | 0.490448 | 7.3:-84598099 | None:intergenic |
| AGATCAACACCCTCCAGTAA+AGG | 0.495696 | 7.3:-84597967 | None:intergenic |
| CTCTGTGATGGTTGGGCGGA+AGG | 0.498808 | 7.3:+84598401 | MS.gene97209:CDS |
| GTTAGTGTCTCTGTGATGGT+TGG | 0.508227 | 7.3:+84598393 | MS.gene97209:CDS |
| CCGTTCAATATAATCGAAGA+TGG | 0.510171 | 7.3:-84598457 | None:intergenic |
| ACGTTGTTGGCGTTTATGGA+AGG | 0.533624 | 7.3:-84598133 | None:intergenic |
| TTAGTGTCTCTGTGATGGTT+GGG | 0.542497 | 7.3:+84598394 | MS.gene97209:CDS |
| AATGTATGTGTGCCAATAGA+TGG | 0.548994 | 7.3:+84598052 | MS.gene97209:CDS |
| TATACTTCTCTTCTCGGAAG+CGG | 0.561703 | 7.3:+84598224 | MS.gene97209:CDS |
| AAGTCACAAACTCCAGAAGC+TGG | 0.569927 | 7.3:-84598282 | None:intergenic |
| GATTACCTAGACATATCCCC+TGG | 0.593211 | 7.3:+84598504 | MS.gene97209:CDS |
| CATCAAAGGAAATTCTCGGA+GGG | 0.597906 | 7.3:-84598167 | None:intergenic |
| GGTAGCCAGGGGATATGTCT+AGG | 0.598963 | 7.3:-84598509 | None:intergenic |
| TCTTTGCTATTCATGGAAGA+GGG | 0.599764 | 7.3:+84598561 | MS.gene97209:CDS |
| CCATCTTCGATTATATTGAA+CGG | 0.603506 | 7.3:+84598457 | MS.gene97209:CDS |
| AACTTGTAGTAGGTTTCAGT+AGG | 0.606356 | 7.3:-84598102 | None:intergenic |
| ACGGTCATCATATAGAGCGT+TGG | 0.609824 | 7.3:+84598476 | MS.gene97209:CDS |
| GAATGTTAGTGTCTCTGTGA+TGG | 0.628771 | 7.3:+84598389 | MS.gene97209:CDS |
| ATAGCAAAGATATCCATGCC+CGG | 0.629559 | 7.3:-84598548 | None:intergenic |
| CCAAATGGATTTCTGTGATG+TGG | 0.629679 | 7.3:+84598614 | MS.gene97209:CDS |
| TCTTGTATTCAGAACTCTCG+AGG | 0.633242 | 7.3:-84598317 | None:intergenic |
| ATTCTAAGGAAGAAGTCTTG+TGG | 0.635806 | 7.3:+84598640 | MS.gene97209:CDS |
| GTGTCTCTGTGATGGTTGGG+CGG | 0.653712 | 7.3:+84598397 | MS.gene97209:CDS |
| TCTGAAGGGATAGTAAACCT+AGG | 0.660259 | 7.3:-84598357 | None:intergenic |
| GTATGGCTGTCTGTAAGCAG+TGG | 0.670737 | 7.3:+84597998 | MS.gene97209:CDS |
| TCATCAAAGGAAATTCTCGG+AGG | 0.698476 | 7.3:-84598168 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATCATCAAAGGAAATTCT+CGG | - | chr7.3:84598174-84598193 | None:intergenic | 25.0% |
| ! | AAGATCTTATGGATGTATTT+GGG | + | chr7.3:84598589-84598608 | MS.gene97209:CDS | 25.0% |
| !!! | GGTTTTATATAACTTGTAGT+AGG | - | chr7.3:84598115-84598134 | None:intergenic | 25.0% |
| CCATCTTCGATTATATTGAA+CGG | + | chr7.3:84598457-84598476 | MS.gene97209:CDS | 30.0% | |
| GAAAAACTCCAAATCATCAA+AGG | - | chr7.3:84598184-84598203 | None:intergenic | 30.0% | |
| GAAGATCTTATGGATGTATT+TGG | + | chr7.3:84598588-84598607 | MS.gene97209:CDS | 30.0% | |
| TGAAGATATACTTCTCTTCT+CGG | + | chr7.3:84598218-84598237 | MS.gene97209:CDS | 30.0% | |
| ! | ACATTCTGATTTTGTTCTGA+AGG | - | chr7.3:84598375-84598394 | None:intergenic | 30.0% |
| ! | GAGAATTTCCTTTGATGATT+TGG | + | chr7.3:84598173-84598192 | MS.gene97209:CDS | 30.0% |
| ! | GGAGTTTTTCATTGACATTT+GGG | + | chr7.3:84598194-84598213 | MS.gene97209:CDS | 30.0% |
| ! | TAGCTAAAGACGATTTCTTT+TGG | + | chr7.3:84598028-84598047 | MS.gene97209:CDS | 30.0% |
| ! | TGAATACAAGATGACTTTTC+CGG | + | chr7.3:84598329-84598348 | MS.gene97209:CDS | 30.0% |
| ! | TGGAGTTTTTCATTGACATT+TGG | + | chr7.3:84598193-84598212 | MS.gene97209:CDS | 30.0% |
| !! | ATGGATATCTTTGCTATTCA+TGG | + | chr7.3:84598554-84598573 | MS.gene97209:CDS | 30.0% |
| !! | CATTCTGATTTTGTTCTGAA+GGG | - | chr7.3:84598374-84598393 | None:intergenic | 30.0% |
| AACTTGTAGTAGGTTTCAGT+AGG | - | chr7.3:84598105-84598124 | None:intergenic | 35.0% | |
| AATGTATGTGTGCCAATAGA+TGG | + | chr7.3:84598052-84598071 | MS.gene97209:CDS | 35.0% | |
| ATAGTAAACCTAGGTTCAAC+CGG | - | chr7.3:84598351-84598370 | None:intergenic | 35.0% | |
| CCGTTCAATATAATCGAAGA+TGG | - | chr7.3:84598460-84598479 | None:intergenic | 35.0% | |
| TGTTGATCTTGCTTCTTGTA+TGG | + | chr7.3:84597981-84598000 | MS.gene97209:CDS | 35.0% | |
| ! | ATTCTAAGGAAGAAGTCTTG+TGG | + | chr7.3:84598640-84598659 | MS.gene97209:CDS | 35.0% |
| !! | ATCTTTGCTATTCATGGAAG+AGG | + | chr7.3:84598560-84598579 | MS.gene97209:CDS | 35.0% |
| !! | GGAATCTGAAGAAGTTTTGA+AGG | + | chr7.3:84597936-84597955 | MS.gene97209:CDS | 35.0% |
| !! | TCTTTGCTATTCATGGAAGA+GGG | + | chr7.3:84598561-84598580 | MS.gene97209:CDS | 35.0% |
| AGAGGGAAGTGAAGATCTTA+TGG | + | chr7.3:84598578-84598597 | MS.gene97209:CDS | 40.0% | |
| ATAGCAAAGATATCCATGCC+CGG | - | chr7.3:84598551-84598570 | None:intergenic | 40.0% | |
| CATCAAAGGAAATTCTCGGA+GGG | - | chr7.3:84598170-84598189 | None:intergenic | 40.0% | |
| CCACATCACAGAAATCCATT+TGG | - | chr7.3:84598617-84598636 | None:intergenic | 40.0% | |
| CTGTGATGTGGCAAATTCTA+AGG | + | chr7.3:84598626-84598645 | MS.gene97209:CDS | 40.0% | |
| GAATGTTAGTGTCTCTGTGA+TGG | + | chr7.3:84598389-84598408 | MS.gene97209:CDS | 40.0% | |
| TAGGTTTCAGTAGGAGGATT+AGG | - | chr7.3:84598096-84598115 | None:intergenic | 40.0% | |
| TATACTTCTCTTCTCGGAAG+CGG | + | chr7.3:84598224-84598243 | MS.gene97209:CDS | 40.0% | |
| TCATCAAAGGAAATTCTCGG+AGG | - | chr7.3:84598171-84598190 | None:intergenic | 40.0% | |
| TCTGAAGGGATAGTAAACCT+AGG | - | chr7.3:84598360-84598379 | None:intergenic | 40.0% | |
| TCTTGTATTCAGAACTCTCG+AGG | - | chr7.3:84598320-84598339 | None:intergenic | 40.0% | |
| TTAGTGTCTCTGTGATGGTT+GGG | + | chr7.3:84598394-84598413 | MS.gene97209:CDS | 40.0% | |
| TTCTACGTTGTTGGCGTTTA+TGG | - | chr7.3:84598140-84598159 | None:intergenic | 40.0% | |
| TTGTAGTAGGTTTCAGTAGG+AGG | - | chr7.3:84598102-84598121 | None:intergenic | 40.0% | |
| ! | CCAAATGGATTTCTGTGATG+TGG | + | chr7.3:84598614-84598633 | MS.gene97209:CDS | 40.0% |
| ! | GGATGTATTTGGGATCCAAA+TGG | + | chr7.3:84598599-84598618 | MS.gene97209:CDS | 40.0% |
| AAGTCACAAACTCCAGAAGC+TGG | - | chr7.3:84598285-84598304 | None:intergenic | 45.0% | |
| ACGGTCATCATATAGAGCGT+TGG | + | chr7.3:84598476-84598495 | MS.gene97209:CDS | 45.0% | |
| ACGTTGTTGGCGTTTATGGA+AGG | - | chr7.3:84598136-84598155 | None:intergenic | 45.0% | |
| AGATCAACACCCTCCAGTAA+AGG | - | chr7.3:84597970-84597989 | None:intergenic | 45.0% | |
| GATCAACACCCTCCAGTAAA+GGG | - | chr7.3:84597969-84597988 | None:intergenic | 45.0% | |
| GATTACCTAGACATATCCCC+TGG | + | chr7.3:84598504-84598523 | MS.gene97209:CDS | 45.0% | |
| GGAGAAGAGTTCTACGTTGT+TGG | - | chr7.3:84598149-84598168 | None:intergenic | 45.0% | |
| GGCTATTGGACATGCTAGAT+TGG | + | chr7.3:84598661-84598680 | MS.gene97209:CDS | 45.0% | |
| GTTAGTGTCTCTGTGATGGT+TGG | + | chr7.3:84598393-84598412 | MS.gene97209:CDS | 45.0% | |
| TGGGCGGAAGGATACAAATA+AGG | + | chr7.3:84598413-84598432 | MS.gene97209:CDS | 45.0% | |
| TTAGGTCGCTTGCAGATTGA+TGG | - | chr7.3:84598078-84598097 | None:intergenic | 45.0% | |
| ! | GCAGATTGATGGCCATCTAT+TGG | - | chr7.3:84598067-84598086 | None:intergenic | 45.0% |
| ! | GGAAGAAGTCTTGTGGCTAT+TGG | + | chr7.3:84598647-84598666 | MS.gene97209:CDS | 45.0% |
| ! | TGACTTTTCCGGTTGAACCT+AGG | + | chr7.3:84598340-84598359 | MS.gene97209:CDS | 45.0% |
| !! | GAAGGCTGTTTTCCCTTTAC+TGG | + | chr7.3:84597954-84597973 | MS.gene97209:CDS | 45.0% |
| !! | GACTTCTGAAACCTGATTGC+AGG | - | chr7.3:84598263-84598282 | None:intergenic | 45.0% |
| !! | GCTGTTTTCCCTTTACTGGA+GGG | + | chr7.3:84597958-84597977 | MS.gene97209:CDS | 45.0% |
| GGTTTCAGAAGTCCAGCTTC+TGG | + | chr7.3:84598270-84598289 | MS.gene97209:CDS | 50.0% | |
| GTATGGCTGTCTGTAAGCAG+TGG | + | chr7.3:84597998-84598017 | MS.gene97209:CDS | 50.0% | |
| !! | GGCTGTTTTCCCTTTACTGG+AGG | + | chr7.3:84597957-84597976 | MS.gene97209:CDS | 50.0% |
| AGATATCCATGCCCGGATGC+CGG | - | chr7.3:84598544-84598563 | None:intergenic | 55.0% | |
| GGTAGCCAGGGGATATGTCT+AGG | - | chr7.3:84598512-84598531 | None:intergenic | 55.0% | |
| GTGTCTCTGTGATGGTTGGG+CGG | + | chr7.3:84598397-84598416 | MS.gene97209:CDS | 55.0% | |
| !! | TCTGGCTCTGTCCTGCAATC+AGG | + | chr7.3:84598249-84598268 | MS.gene97209:CDS | 55.0% |
| CGGGCAAAAAGGGGTAGCCA+GGG | - | chr7.3:84598524-84598543 | None:intergenic | 60.0% | |
| CTCTGTGATGGTTGGGCGGA+AGG | + | chr7.3:84598401-84598420 | MS.gene97209:CDS | 60.0% | |
| CTCTTCTCGGAAGCGGTGTC+TGG | + | chr7.3:84598231-84598250 | MS.gene97209:CDS | 60.0% | |
| GATATCCATGCCCGGATGCC+GGG | - | chr7.3:84598543-84598562 | None:intergenic | 60.0% | |
| GGGCAAAAAGGGGTAGCCAG+GGG | - | chr7.3:84598523-84598542 | None:intergenic | 60.0% | |
| ! | ACCCCTTTTTGCCCGGCATC+CGG | + | chr7.3:84598529-84598548 | MS.gene97209:CDS | 60.0% |
| ! | CCTGGCTACCCCTTTTTGCC+CGG | + | chr7.3:84598522-84598541 | MS.gene97209:CDS | 60.0% |
| CCCGGATGCCGGGCAAAAAG+GGG | - | chr7.3:84598533-84598552 | None:intergenic | 65.0% | |
| CCGGGCAAAAAGGGGTAGCC+AGG | - | chr7.3:84598525-84598544 | None:intergenic | 65.0% | |
| GCCCGGATGCCGGGCAAAAA+GGG | - | chr7.3:84598534-84598553 | None:intergenic | 65.0% | |
| TGCCCGGATGCCGGGCAAAA+AGG | - | chr7.3:84598535-84598554 | None:intergenic | 65.0% | |
| ! | CCCCTTTTTGCCCGGCATCC+GGG | + | chr7.3:84598530-84598549 | MS.gene97209:CDS | 65.0% |
| ! | TTTTGCCCGGCATCCGGGCA+TGG | + | chr7.3:84598535-84598554 | MS.gene97209:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 84597934 | 84598689 | 84597934 | ID=MS.gene97209 |
| chr7.3 | mRNA | 84597934 | 84598689 | 84597934 | ID=MS.gene97209.t1;Parent=MS.gene97209 |
| chr7.3 | exon | 84597934 | 84598689 | 84597934 | ID=MS.gene97209.t1.exon1;Parent=MS.gene97209.t1 |
| chr7.3 | CDS | 84597934 | 84598689 | 84597934 | ID=cds.MS.gene97209.t1;Parent=MS.gene97209.t1 |
| Gene Sequence |
| Protein sequence |
