Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97310.t1 | XP_024626527.1 | 96.5 | 231 | 8 | 0 | 1 | 231 | 1 | 231 | 1.20E-122 | 449.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97310.t1 | Q921X6 | 24.7 | 243 | 154 | 4 | 12 | 231 | 80 | 316 | 6.3e-19 | 95.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97310.t1 | A0A396GUA7 | 96.5 | 231 | 8 | 0 | 1 | 231 | 1 | 231 | 8.8e-123 | 449.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053008 | MS.gene97310 | 0.81805 | 2.40E-52 | -1.69E-46 |
| MS.gene05462 | MS.gene97310 | 0.804031 | 2.63E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97310.t1 | MTR_0003s0690 | 93.642 | 173 | 11 | 0 | 59 | 231 | 1 | 173 | 6.29e-121 | 341 |
| MS.gene97310.t1 | MTR_7g109780 | 73.057 | 193 | 47 | 2 | 44 | 231 | 1 | 193 | 2.91e-101 | 292 |
| MS.gene97310.t1 | MTR_7g109760 | 42.771 | 166 | 38 | 5 | 1 | 164 | 1 | 111 | 2.80e-31 | 111 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97310.t1 | AT5G23710 | 37.209 | 215 | 128 | 3 | 21 | 231 | 19 | 230 | 1.34e-47 | 157 |
Find 37 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGATGACAAGTACTGGATA+TGG | 0.326445 | 7.3:+78741728 | MS.gene97310:CDS |
| ATGAAACGTTTGCAAGAATC+TGG | 0.362818 | 7.3:+78741236 | MS.gene97310:CDS |
| GATGTTTGGAGTGGGTTAAA+AGG | 0.362955 | 7.3:+78741624 | MS.gene97310:CDS |
| GGCACTTATAAACAAGAGTA+TGG | 0.381632 | 7.3:+78741415 | MS.gene97310:CDS |
| GAGCCTTCCAAGGATATAAC+TGG | 0.383383 | 7.3:+78741497 | MS.gene97310:CDS |
| TGAAAATAGACACATGTTGT+TGG | 0.391859 | 7.3:-78741890 | None:intergenic |
| TTCCACATGTGATGGATGTT+TGG | 0.402495 | 7.3:+78741610 | MS.gene97310:CDS |
| TAGAGTATACGCTCTTCATC+TGG | 0.446970 | 7.3:-78741296 | None:intergenic |
| TGTTTATTAAGCGCAGCAAT+AGG | 0.447212 | 7.3:-78741386 | None:intergenic |
| ATGCCCACCAGTTATATCCT+TGG | 0.459091 | 7.3:-78741504 | None:intergenic |
| TGGAGTGGGTTAAAAGGAGT+GGG | 0.480413 | 7.3:+78741630 | MS.gene97310:CDS |
| AGATGACAAGTACTGGATAT+GGG | 0.485810 | 7.3:+78741729 | MS.gene97310:CDS |
| CAAAGGTATAAAGATCTACA+TGG | 0.502338 | 7.3:+78741463 | MS.gene97310:CDS |
| ATAATGCAGATGACAAGTAC+TGG | 0.506988 | 7.3:+78741722 | MS.gene97310:CDS |
| ATACTTAGCAAGGAAAACTC+AGG | 0.536886 | 7.3:+78741326 | MS.gene97310:CDS |
| TATAAACAAGAGTATGGTAA+AGG | 0.546896 | 7.3:+78741421 | MS.gene97310:CDS |
| GAGAGAGTTTCCACATGTGA+TGG | 0.556572 | 7.3:+78741602 | MS.gene97310:CDS |
| GTGCTTGCAATGCATTTCAT+CGG | 0.557450 | 7.3:+78741580 | MS.gene97310:CDS |
| TTGGAGTGGGTTAAAAGGAG+TGG | 0.562912 | 7.3:+78741629 | MS.gene97310:CDS |
| AACAGAATTTGAGCCTTCCA+AGG | 0.568058 | 7.3:+78741487 | MS.gene97310:CDS |
| TATTAAGCGCAGCAATAGGA+AGG | 0.568531 | 7.3:-78741382 | None:intergenic |
| TTGTTACATATGCAAAAGCA+AGG | 0.588438 | 7.3:+78741781 | MS.gene97310:CDS |
| GTCATCACAACTCAAGTCAC+GGG | 0.589333 | 7.3:+78741653 | MS.gene97310:CDS |
| TGCGCTTAATAAACACATGA+AGG | 0.598707 | 7.3:+78741394 | MS.gene97310:CDS |
| CTATGACCTTATACTTAGCA+AGG | 0.601401 | 7.3:+78741316 | MS.gene97310:CDS |
| CCACCAGTTATATCCTTGGA+AGG | 0.602371 | 7.3:-78741500 | None:intergenic |
| GGTCATCACAACTCAAGTCA+CGG | 0.608686 | 7.3:+78741652 | MS.gene97310:CDS |
| CCTTCCAAGGATATAACTGG+TGG | 0.618508 | 7.3:+78741500 | MS.gene97310:CDS |
| CTCCAAACATCCATCACATG+TGG | 0.618959 | 7.3:-78741612 | None:intergenic |
| TACTTAGCAAGGAAAACTCA+GGG | 0.624253 | 7.3:+78741327 | MS.gene97310:CDS |
| GATGACAAGTACTGGATATG+GGG | 0.631855 | 7.3:+78741730 | MS.gene97310:CDS |
| GGAGTGGGTTAAAAGGAGTG+GGG | 0.639610 | 7.3:+78741631 | MS.gene97310:CDS |
| CATGTGATGGATGTTTGGAG+TGG | 0.639686 | 7.3:+78741615 | MS.gene97310:CDS |
| ATGTGATGGATGTTTGGAGT+GGG | 0.644389 | 7.3:+78741616 | MS.gene97310:CDS |
| TGTTACATATGCAAAAGCAA+GGG | 0.651007 | 7.3:+78741782 | MS.gene97310:CDS |
| AAAGGTATAAAGATCTACAT+GGG | 0.652631 | 7.3:+78741464 | MS.gene97310:CDS |
| CTTCCAAGGATATAACTGGT+GGG | 0.673279 | 7.3:+78741501 | MS.gene97310:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTTTATACCTTTGTTTTTAA+TGG | - | chr7.3:78741456-78741475 | None:intergenic | 20.0% |
| !! | AAAGGTATAAAGATCTACAT+GGG | + | chr7.3:78741464-78741483 | MS.gene97310:CDS | 25.0% |
| !! | TATAAACAAGAGTATGGTAA+AGG | + | chr7.3:78741421-78741440 | MS.gene97310:CDS | 25.0% |
| !!! | ATACCTTTGTTTTTAATGGA+AGG | - | chr7.3:78741452-78741471 | None:intergenic | 25.0% |
| CTTCCTTCCATTAAAAACAA+AGG | + | chr7.3:78741446-78741465 | MS.gene97310:CDS | 30.0% | |
| TGAAAATAGACACATGTTGT+TGG | - | chr7.3:78741893-78741912 | None:intergenic | 30.0% | |
| TGACAAGAAAAACATGGAAA+GGG | - | chr7.3:78741833-78741852 | None:intergenic | 30.0% | |
| TGTTACATATGCAAAAGCAA+GGG | + | chr7.3:78741782-78741801 | MS.gene97310:CDS | 30.0% | |
| TTGACAAGAAAAACATGGAA+AGG | - | chr7.3:78741834-78741853 | None:intergenic | 30.0% | |
| TTGTTACATATGCAAAAGCA+AGG | + | chr7.3:78741781-78741800 | MS.gene97310:CDS | 30.0% | |
| ! | AGAAGAGATTTTGCAAACTT+TGG | + | chr7.3:78741685-78741704 | MS.gene97310:CDS | 30.0% |
| ! | AGTTTTCCTTGCTAAGTATA+AGG | - | chr7.3:78741325-78741344 | None:intergenic | 30.0% |
| ! | CAAAGGTATAAAGATCTACA+TGG | + | chr7.3:78741463-78741482 | MS.gene97310:CDS | 30.0% |
| ! | CATGTGTCTATTTTCAGAAA+TGG | + | chr7.3:78741897-78741916 | MS.gene97310:CDS | 30.0% |
| ! | TGTCTATTTTCAGAAATGGT+TGG | + | chr7.3:78741901-78741920 | MS.gene97310:CDS | 30.0% |
| ! | TTCCATGTTTTTCTTGTCAA+CGG | + | chr7.3:78741834-78741853 | MS.gene97310:CDS | 30.0% |
| ACAGAAACGACAAAAACTGT+CGG | + | chr7.3:78741268-78741287 | MS.gene97310:CDS | 35.0% | |
| ATAATGCAGATGACAAGTAC+TGG | + | chr7.3:78741722-78741741 | MS.gene97310:CDS | 35.0% | |
| ATACTTAGCAAGGAAAACTC+AGG | + | chr7.3:78741326-78741345 | MS.gene97310:CDS | 35.0% | |
| ATCCGTTGACAAGAAAAACA+TGG | - | chr7.3:78741839-78741858 | None:intergenic | 35.0% | |
| ATGAAACGTTTGCAAGAATC+TGG | + | chr7.3:78741236-78741255 | MS.gene97310:CDS | 35.0% | |
| CTATGACCTTATACTTAGCA+AGG | + | chr7.3:78741316-78741335 | MS.gene97310:CDS | 35.0% | |
| GGCACTTATAAACAAGAGTA+TGG | + | chr7.3:78741415-78741434 | MS.gene97310:CDS | 35.0% | |
| TACTTAGCAAGGAAAACTCA+GGG | + | chr7.3:78741327-78741346 | MS.gene97310:CDS | 35.0% | |
| TGCGCTTAATAAACACATGA+AGG | + | chr7.3:78741394-78741413 | MS.gene97310:CDS | 35.0% | |
| TGTTTATTAAGCGCAGCAAT+AGG | - | chr7.3:78741389-78741408 | None:intergenic | 35.0% | |
| ! | ATGTAACAAGTTTTGCCAAC+AGG | - | chr7.3:78741770-78741789 | None:intergenic | 35.0% |
| ! | GATTTTGCATCTATTCCTGT+TGG | + | chr7.3:78741752-78741771 | MS.gene97310:CDS | 35.0% |
| !! | AGATGACAAGTACTGGATAT+GGG | + | chr7.3:78741729-78741748 | MS.gene97310:CDS | 35.0% |
| !! | TTTTTGTCGTTTCTGTTGAG+AGG | - | chr7.3:78741264-78741283 | None:intergenic | 35.0% |
| AACAGAATTTGAGCCTTCCA+AGG | + | chr7.3:78741487-78741506 | MS.gene97310:CDS | 40.0% | |
| CTTCCAAGGATATAACTGGT+GGG | + | chr7.3:78741501-78741520 | MS.gene97310:CDS | 40.0% | |
| GTGCTTGCAATGCATTTCAT+CGG | + | chr7.3:78741580-78741599 | MS.gene97310:CDS | 40.0% | |
| TAGAGTATACGCTCTTCATC+TGG | - | chr7.3:78741299-78741318 | None:intergenic | 40.0% | |
| TATTAAGCGCAGCAATAGGA+AGG | - | chr7.3:78741385-78741404 | None:intergenic | 40.0% | |
| ! | ACGGATGAGCTTTTGTTCAA+CGG | + | chr7.3:78741853-78741872 | MS.gene97310:CDS | 40.0% |
| ! | ATGAGCTTTTGTTCAACGGA+TGG | + | chr7.3:78741857-78741876 | MS.gene97310:CDS | 40.0% |
| !! | ATGTGATGGATGTTTGGAGT+GGG | + | chr7.3:78741616-78741635 | MS.gene97310:CDS | 40.0% |
| !! | CAGATGACAAGTACTGGATA+TGG | + | chr7.3:78741728-78741747 | MS.gene97310:CDS | 40.0% |
| !! | GATGACAAGTACTGGATATG+GGG | + | chr7.3:78741730-78741749 | MS.gene97310:CDS | 40.0% |
| !! | GATGTTTGGAGTGGGTTAAA+AGG | + | chr7.3:78741624-78741643 | MS.gene97310:CDS | 40.0% |
| !! | TTCCACATGTGATGGATGTT+TGG | + | chr7.3:78741610-78741629 | MS.gene97310:CDS | 40.0% |
| ATGCCCACCAGTTATATCCT+TGG | - | chr7.3:78741507-78741526 | None:intergenic | 45.0% | |
| CCACCAGTTATATCCTTGGA+AGG | - | chr7.3:78741503-78741522 | None:intergenic | 45.0% | |
| CCTTCCAAGGATATAACTGG+TGG | + | chr7.3:78741500-78741519 | MS.gene97310:CDS | 45.0% | |
| CTCCAAACATCCATCACATG+TGG | - | chr7.3:78741615-78741634 | None:intergenic | 45.0% | |
| GAGAGAGTTTCCACATGTGA+TGG | + | chr7.3:78741602-78741621 | MS.gene97310:CDS | 45.0% | |
| GAGCCTTCCAAGGATATAAC+TGG | + | chr7.3:78741497-78741516 | MS.gene97310:CDS | 45.0% | |
| GGTCATCACAACTCAAGTCA+CGG | + | chr7.3:78741652-78741671 | MS.gene97310:CDS | 45.0% | |
| GTCATCACAACTCAAGTCAC+GGG | + | chr7.3:78741653-78741672 | MS.gene97310:CDS | 45.0% | |
| ! | GTGGGCATTTTTACAGCGAA+GGG | + | chr7.3:78741519-78741538 | MS.gene97310:CDS | 45.0% |
| !! | CATGTGATGGATGTTTGGAG+TGG | + | chr7.3:78741615-78741634 | MS.gene97310:CDS | 45.0% |
| !! | TGGAGTGGGTTAAAAGGAGT+GGG | + | chr7.3:78741630-78741649 | MS.gene97310:CDS | 45.0% |
| !! | TTGGAGTGGGTTAAAAGGAG+TGG | + | chr7.3:78741629-78741648 | MS.gene97310:CDS | 45.0% |
| ! | GGTGGGCATTTTTACAGCGA+AGG | + | chr7.3:78741518-78741537 | MS.gene97310:CDS | 50.0% |
| !! | GGAGTGGGTTAAAAGGAGTG+GGG | + | chr7.3:78741631-78741650 | MS.gene97310:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 78741236 | 78741931 | 78741236 | ID=MS.gene97310 |
| chr7.3 | mRNA | 78741236 | 78741931 | 78741236 | ID=MS.gene97310.t1;Parent=MS.gene97310 |
| chr7.3 | exon | 78741236 | 78741931 | 78741236 | ID=MS.gene97310.t1.exon1;Parent=MS.gene97310.t1 |
| chr7.3 | CDS | 78741236 | 78741931 | 78741236 | ID=cds.MS.gene97310.t1;Parent=MS.gene97310.t1 |
| Gene Sequence |
| Protein sequence |
