Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97659.t1 | XP_003606132.1 | 85.9 | 170 | 20 | 1 | 1 | 166 | 1 | 170 | 4.00E-75 | 290.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97659.t1 | Q9SJM6 | 49.4 | 168 | 76 | 2 | 1 | 166 | 1 | 161 | 3.9e-39 | 162.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97659.t1 | G7JIG0 | 85.9 | 170 | 20 | 1 | 1 | 166 | 1 | 170 | 2.9e-75 | 290.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene97659 | MS.gene050335 | PPI |
| MS.gene97659 | MS.gene24724 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97659.t1 | MTR_4g053440 | 94.118 | 170 | 6 | 2 | 1 | 166 | 1 | 170 | 7.61e-115 | 323 |
| MS.gene97659.t1 | MTR_2g086190 | 57.229 | 166 | 55 | 3 | 1 | 166 | 1 | 150 | 8.63e-59 | 180 |
| MS.gene97659.t1 | MTR_7g104320 | 51.136 | 176 | 68 | 4 | 1 | 166 | 1 | 168 | 2.49e-57 | 177 |
| MS.gene97659.t1 | MTR_1g060380 | 50.292 | 171 | 65 | 4 | 1 | 166 | 1 | 156 | 4.24e-47 | 150 |
| MS.gene97659.t1 | MTR_2g098160 | 41.520 | 171 | 83 | 5 | 7 | 166 | 8 | 172 | 1.37e-40 | 134 |
| MS.gene97659.t1 | MTR_0249s0070 | 42.012 | 169 | 89 | 5 | 3 | 166 | 5 | 169 | 3.82e-39 | 131 |
| MS.gene97659.t1 | MTR_0249s0070 | 42.012 | 169 | 89 | 5 | 3 | 166 | 13 | 177 | 4.81e-39 | 131 |
| MS.gene97659.t1 | MTR_7g092400 | 39.011 | 182 | 79 | 5 | 3 | 166 | 93 | 260 | 1.33e-37 | 129 |
| MS.gene97659.t1 | MTR_7g114920 | 40.789 | 152 | 63 | 5 | 13 | 163 | 5 | 130 | 2.46e-31 | 110 |
| MS.gene97659.t1 | MTR_4g065570 | 37.931 | 145 | 75 | 3 | 28 | 166 | 2 | 137 | 8.84e-31 | 108 |
| MS.gene97659.t1 | MTR_1g100773 | 39.073 | 151 | 74 | 4 | 13 | 163 | 5 | 137 | 5.80e-30 | 107 |
| MS.gene97659.t1 | MTR_3g028010 | 33.766 | 154 | 84 | 2 | 13 | 166 | 28 | 163 | 1.91e-24 | 93.2 |
| MS.gene97659.t1 | MTR_8g036980 | 56.452 | 62 | 27 | 0 | 105 | 166 | 90 | 151 | 1.69e-22 | 88.2 |
| MS.gene97659.t1 | MTR_2g054650 | 38.158 | 152 | 60 | 7 | 13 | 163 | 6 | 124 | 1.00e-19 | 80.1 |
| MS.gene97659.t1 | MTR_3g025570 | 54.545 | 44 | 20 | 0 | 101 | 144 | 4 | 47 | 6.55e-12 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97659.t1 | AT2G27580 | 56.627 | 166 | 69 | 2 | 1 | 166 | 1 | 163 | 9.75e-56 | 173 |
| MS.gene97659.t1 | AT2G27580 | 56.627 | 166 | 69 | 2 | 1 | 166 | 1 | 163 | 9.75e-56 | 173 |
| MS.gene97659.t1 | AT2G36320 | 49.405 | 168 | 76 | 2 | 1 | 166 | 1 | 161 | 9.54e-52 | 162 |
| MS.gene97659.t1 | AT1G12440 | 45.806 | 155 | 81 | 1 | 12 | 166 | 17 | 168 | 1.50e-45 | 147 |
| MS.gene97659.t1 | AT1G12440 | 45.806 | 155 | 81 | 1 | 12 | 166 | 17 | 168 | 1.50e-45 | 147 |
| MS.gene97659.t1 | AT1G12440 | 45.806 | 155 | 81 | 1 | 12 | 166 | 17 | 168 | 1.50e-45 | 147 |
| MS.gene97659.t1 | AT3G52800 | 48.276 | 174 | 78 | 3 | 1 | 166 | 1 | 170 | 3.06e-43 | 141 |
| MS.gene97659.t1 | AT3G52800 | 48.276 | 174 | 78 | 3 | 1 | 166 | 1 | 170 | 3.06e-43 | 141 |
| MS.gene97659.t1 | AT4G12040 | 43.529 | 170 | 70 | 4 | 12 | 166 | 17 | 175 | 5.16e-42 | 138 |
| MS.gene97659.t1 | AT4G12040 | 43.529 | 170 | 70 | 4 | 12 | 166 | 17 | 175 | 5.16e-42 | 138 |
| MS.gene97659.t1 | AT4G22820 | 44.099 | 161 | 80 | 4 | 12 | 166 | 20 | 176 | 9.87e-42 | 138 |
| MS.gene97659.t1 | AT4G22820 | 44.099 | 161 | 80 | 4 | 12 | 166 | 20 | 176 | 9.87e-42 | 138 |
| MS.gene97659.t1 | AT1G51200 | 44.099 | 161 | 81 | 4 | 12 | 166 | 16 | 173 | 1.16e-37 | 127 |
| MS.gene97659.t1 | AT1G51200 | 44.099 | 161 | 81 | 4 | 12 | 166 | 16 | 173 | 1.16e-37 | 127 |
| MS.gene97659.t1 | AT1G51200 | 44.099 | 161 | 81 | 4 | 12 | 166 | 16 | 173 | 1.16e-37 | 127 |
| MS.gene97659.t1 | AT1G51200 | 44.099 | 161 | 81 | 4 | 12 | 166 | 16 | 173 | 1.16e-37 | 127 |
| MS.gene97659.t1 | AT3G12630 | 34.211 | 152 | 74 | 4 | 19 | 166 | 31 | 160 | 1.61e-24 | 93.6 |
| MS.gene97659.t1 | AT4G25380 | 34.641 | 153 | 68 | 6 | 14 | 166 | 10 | 130 | 4.06e-23 | 89.0 |
| MS.gene97659.t1 | AT4G14225 | 31.613 | 155 | 69 | 2 | 13 | 166 | 7 | 125 | 2.83e-20 | 81.6 |
Find 51 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACGTTCTTCATCTGCAAAAT+TGG | 0.245276 | 4.4:+48999829 | MS.gene97659:CDS |
| GCTAACAACTGCGGTTTCTT+TGG | 0.331458 | 4.4:+48999746 | MS.gene97659:CDS |
| TATCCGGAGCAGCACGGTTG+TGG | 0.350405 | 4.4:+49000100 | MS.gene97659:CDS |
| CTGCAAATCACGGTAACATT+TGG | 0.369389 | 4.4:-48999795 | None:intergenic |
| AGCTTCTCTCCCTTCACCAC+TGG | 0.384998 | 4.4:-49000169 | None:intergenic |
| CTGCATGTCCCACATCTATT+CGG | 0.389492 | 4.4:-49000013 | None:intergenic |
| CATCTATTCGGCTGGACCTT+CGG | 0.396216 | 4.4:-49000001 | None:intergenic |
| GTGGAGTTGGTAGCTGCTGC+TGG | 0.406487 | 4.4:+48999959 | MS.gene97659:CDS |
| GTGTGGGACCCACAGGTATC+CGG | 0.436288 | 4.4:+49000084 | MS.gene97659:CDS |
| ACTGTTGAGGAATCAACGTT+TGG | 0.447132 | 4.4:-48999859 | None:intergenic |
| GAGCAAAGATCTTGCATGGC+AGG | 0.449620 | 4.4:-48999773 | None:intergenic |
| AACCCACAACCGTGCTGCTC+CGG | 0.450007 | 4.4:-49000103 | None:intergenic |
| GGTAGCTGCTGCTGGACCTT+CGG | 0.453896 | 4.4:+48999967 | MS.gene97659:CDS |
| AGACGGCGCGTGGGGCTGAC+AGG | 0.473736 | 4.4:+49000034 | MS.gene97659:CDS |
| TGGGACATGCAGACGGCGCG+TGG | 0.478294 | 4.4:+49000024 | MS.gene97659:CDS |
| ATCCGGAGCAGCACGGTTGT+GGG | 0.489485 | 4.4:+49000101 | MS.gene97659:CDS |
| TGACGTTGTGTGGGACCCAC+AGG | 0.525772 | 4.4:+49000077 | MS.gene97659:CDS |
| ACTACAACCGACGGTGGAGA+TGG | 0.535288 | 4.4:-48999917 | None:intergenic |
| CGTGCTGCTCCGGATACCTG+TGG | 0.543879 | 4.4:-49000093 | None:intergenic |
| GAAGGCGAATCCAGTGGTGA+AGG | 0.546816 | 4.4:+49000159 | MS.gene97659:CDS |
| ATGTCCCACATCTATTCGGC+TGG | 0.547042 | 4.4:-49000009 | None:intergenic |
| ATCTACACCGACGGTGGAGT+TGG | 0.547793 | 4.4:+48999946 | MS.gene97659:CDS |
| AAGGTCCAGCCGAATAGATG+TGG | 0.556145 | 4.4:+49000004 | MS.gene97659:CDS |
| TTCGGTGGTTCAGCCTCCGA+AGG | 0.562954 | 4.4:-48999983 | None:intergenic |
| CAAGAAGAATTAGACTGTTG+AGG | 0.566912 | 4.4:-48999872 | None:intergenic |
| GGCTGACAGGATTTAAGTGT+CGG | 0.567077 | 4.4:+49000047 | MS.gene97659:CDS |
| AGCTGCTGCTGGACCTTCGG+AGG | 0.570808 | 4.4:+48999970 | MS.gene97659:CDS |
| GTTCTTTCATCTGCAAATCA+CGG | 0.573796 | 4.4:-48999805 | None:intergenic |
| TTTGGAGCAAAGATCTTGCA+TGG | 0.576644 | 4.4:-48999777 | None:intergenic |
| GGGACATGCAGACGGCGCGT+GGG | 0.585572 | 4.4:+49000025 | MS.gene97659:CDS |
| CGGTGCGGATTGACGTTGTG+TGG | 0.586129 | 4.4:+49000067 | MS.gene97659:CDS |
| GGACATGCAGACGGCGCGTG+GGG | 0.594994 | 4.4:+49000026 | MS.gene97659:CDS |
| GTGCTGCTCCGGATACCTGT+GGG | 0.602557 | 4.4:-49000092 | None:intergenic |
| TATTCATCCATCTCCACCGT+CGG | 0.610000 | 4.4:+48999910 | MS.gene97659:CDS |
| AAGGCGAATCCAGTGGTGAA+GGG | 0.610190 | 4.4:+49000160 | MS.gene97659:CDS |
| CTATTCGGCTGGACCTTCGG+TGG | 0.610641 | 4.4:-48999998 | None:intergenic |
| TTCGGAGGCTGAACCACCGA+AGG | 0.620244 | 4.4:+48999985 | MS.gene97659:CDS |
| TAATTCTTCTTGTCTAGACA+CGG | 0.623144 | 4.4:+48999883 | MS.gene97659:CDS |
| GGTGCGGATTGACGTTGTGT+GGG | 0.623964 | 4.4:+49000068 | MS.gene97659:CDS |
| GCAGCTACCAACTCCACCGT+CGG | 0.632431 | 4.4:-48999953 | None:intergenic |
| ATAGATGTGGGACATGCAGA+CGG | 0.633727 | 4.4:+49000017 | MS.gene97659:CDS |
| AGTTTCTTCATCTACACCGA+CGG | 0.637393 | 4.4:+48999937 | MS.gene97659:CDS |
| TTCTTCATCTACACCGACGG+TGG | 0.645268 | 4.4:+48999940 | MS.gene97659:CDS |
| AGGTCCAGCCGAATAGATGT+GGG | 0.648986 | 4.4:+49000005 | MS.gene97659:CDS |
| GGGAAGGGAGGAGATCAAGA+AGG | 0.649649 | 4.4:+49000141 | MS.gene97659:CDS |
| CACAGGTATCCGGAGCAGCA+CGG | 0.653832 | 4.4:+49000094 | MS.gene97659:CDS |
| GATCAAGAAGGCGAATCCAG+TGG | 0.675101 | 4.4:+49000153 | MS.gene97659:CDS |
| ACAGGATTTAAGTGTCGGTG+CGG | 0.676149 | 4.4:+49000052 | MS.gene97659:CDS |
| GATGAAGAAACTACAACCGA+CGG | 0.679990 | 4.4:-48999926 | None:intergenic |
| CGTCTCTGTGCTAACAACTG+CGG | 0.696402 | 4.4:+48999737 | MS.gene97659:CDS |
| GAAGAAACTACAACCGACGG+TGG | 0.716652 | 4.4:-48999923 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TAATTCTTCTTGTCTAGACA+CGG | + | chr4.4:48999883-48999902 | MS.gene97659:CDS | 30.0% | |
| ACGTTCTTCATCTGCAAAAT+TGG | + | chr4.4:48999829-48999848 | MS.gene97659:CDS | 35.0% | |
| CAAGAAGAATTAGACTGTTG+AGG | - | chr4.4:48999875-48999894 | None:intergenic | 35.0% | |
| GTTCTTTCATCTGCAAATCA+CGG | - | chr4.4:48999808-48999827 | None:intergenic | 35.0% | |
| !! | GGTTGTGGGTTTGATTTTAA+GGG | + | chr4.4:49000115-49000134 | MS.gene97659:CDS | 35.0% |
| !! | GTTGTGGGTTTGATTTTAAG+GGG | + | chr4.4:49000116-49000135 | MS.gene97659:CDS | 35.0% |
| ACTGTTGAGGAATCAACGTT+TGG | - | chr4.4:48999862-48999881 | None:intergenic | 40.0% | |
| AGTTTCTTCATCTACACCGA+CGG | + | chr4.4:48999937-48999956 | MS.gene97659:CDS | 40.0% | |
| CTGCAAATCACGGTAACATT+TGG | - | chr4.4:48999798-48999817 | None:intergenic | 40.0% | |
| GATGAAGAAACTACAACCGA+CGG | - | chr4.4:48999929-48999948 | None:intergenic | 40.0% | |
| ! | TTTGGAGCAAAGATCTTGCA+TGG | - | chr4.4:48999780-48999799 | None:intergenic | 40.0% |
| !! | CGGTTGTGGGTTTGATTTTA+AGG | + | chr4.4:49000114-49000133 | MS.gene97659:CDS | 40.0% |
| !! | GGGTTTGATTTTAAGGGGAT+GGG | + | chr4.4:49000121-49000140 | MS.gene97659:CDS | 40.0% |
| !! | TGATTTTAAGGGGATGGGAA+GGG | + | chr4.4:49000126-49000145 | MS.gene97659:CDS | 40.0% |
| !! | TGGGTTTGATTTTAAGGGGA+TGG | + | chr4.4:49000120-49000139 | MS.gene97659:CDS | 40.0% |
| !! | TTGATTTTAAGGGGATGGGA+AGG | + | chr4.4:49000125-49000144 | MS.gene97659:CDS | 40.0% |
| ATAGATGTGGGACATGCAGA+CGG | + | chr4.4:49000017-49000036 | MS.gene97659:CDS | 45.0% | |
| CTGCATGTCCCACATCTATT+CGG | - | chr4.4:49000016-49000035 | None:intergenic | 45.0% | |
| GCTAACAACTGCGGTTTCTT+TGG | + | chr4.4:48999746-48999765 | MS.gene97659:CDS | 45.0% | |
| GGCTGACAGGATTTAAGTGT+CGG | + | chr4.4:49000047-49000066 | MS.gene97659:CDS | 45.0% | |
| TATTCATCCATCTCCACCGT+CGG | + | chr4.4:48999910-48999929 | MS.gene97659:CDS | 45.0% | |
| !! | ACAGGATTTAAGTGTCGGTG+CGG | + | chr4.4:49000052-49000071 | MS.gene97659:CDS | 45.0% |
| AAGGCGAATCCAGTGGTGAA+GGG | + | chr4.4:49000160-49000179 | MS.gene97659:CDS | 50.0% | |
| AAGGTCCAGCCGAATAGATG+TGG | + | chr4.4:49000004-49000023 | MS.gene97659:CDS | 50.0% | |
| AGGTCCAGCCGAATAGATGT+GGG | + | chr4.4:49000005-49000024 | MS.gene97659:CDS | 50.0% | |
| ATGTCCCACATCTATTCGGC+TGG | - | chr4.4:49000012-49000031 | None:intergenic | 50.0% | |
| CATCTATTCGGCTGGACCTT+CGG | - | chr4.4:49000004-49000023 | None:intergenic | 50.0% | |
| CGTCTCTGTGCTAACAACTG+CGG | + | chr4.4:48999737-48999756 | MS.gene97659:CDS | 50.0% | |
| GAAGAAACTACAACCGACGG+TGG | - | chr4.4:48999926-48999945 | None:intergenic | 50.0% | |
| GAGCAAAGATCTTGCATGGC+AGG | - | chr4.4:48999776-48999795 | None:intergenic | 50.0% | |
| GATCAAGAAGGCGAATCCAG+TGG | + | chr4.4:49000153-49000172 | MS.gene97659:CDS | 50.0% | |
| TTCTTCATCTACACCGACGG+TGG | + | chr4.4:48999940-48999959 | MS.gene97659:CDS | 50.0% | |
| !! | TTTTAAGGGGATGGGAAGGG+AGG | + | chr4.4:49000129-49000148 | MS.gene97659:CDS | 50.0% |
| ACTACAACCGACGGTGGAGA+TGG | - | chr4.4:48999920-48999939 | None:intergenic | 55.0% | |
| AGCTTCTCTCCCTTCACCAC+TGG | - | chr4.4:49000172-49000191 | None:intergenic | 55.0% | |
| ATCTACACCGACGGTGGAGT+TGG | + | chr4.4:48999946-48999965 | MS.gene97659:CDS | 55.0% | |
| GAAGGCGAATCCAGTGGTGA+AGG | + | chr4.4:49000159-49000178 | MS.gene97659:CDS | 55.0% | |
| GGGAAGGGAGGAGATCAAGA+AGG | + | chr4.4:49000141-49000160 | MS.gene97659:CDS | 55.0% | |
| ! | GGTGCGGATTGACGTTGTGT+GGG | + | chr4.4:49000068-49000087 | MS.gene97659:CDS | 55.0% |
| ATCCGGAGCAGCACGGTTGT+GGG | + | chr4.4:49000101-49000120 | MS.gene97659:CDS | 60.0% | |
| CACAGGTATCCGGAGCAGCA+CGG | + | chr4.4:49000094-49000113 | MS.gene97659:CDS | 60.0% | |
| CTATTCGGCTGGACCTTCGG+TGG | - | chr4.4:49000001-49000020 | None:intergenic | 60.0% | |
| GCAGCTACCAACTCCACCGT+CGG | - | chr4.4:48999956-48999975 | None:intergenic | 60.0% | |
| GGTAGCTGCTGCTGGACCTT+CGG | + | chr4.4:48999967-48999986 | MS.gene97659:CDS | 60.0% | |
| GTGTGGGACCCACAGGTATC+CGG | + | chr4.4:49000084-49000103 | MS.gene97659:CDS | 60.0% | |
| TATCCGGAGCAGCACGGTTG+TGG | + | chr4.4:49000100-49000119 | MS.gene97659:CDS | 60.0% | |
| TGACGTTGTGTGGGACCCAC+AGG | + | chr4.4:49000077-49000096 | MS.gene97659:CDS | 60.0% | |
| TTCGGAGGCTGAACCACCGA+AGG | + | chr4.4:48999985-49000004 | MS.gene97659:CDS | 60.0% | |
| TTCGGTGGTTCAGCCTCCGA+AGG | - | chr4.4:48999986-49000005 | None:intergenic | 60.0% | |
| ! | AACCCACAACCGTGCTGCTC+CGG | - | chr4.4:49000106-49000125 | None:intergenic | 60.0% |
| ! | CGGTGCGGATTGACGTTGTG+TGG | + | chr4.4:49000067-49000086 | MS.gene97659:CDS | 60.0% |
| ! | GTGCTGCTCCGGATACCTGT+GGG | - | chr4.4:49000095-49000114 | None:intergenic | 60.0% |
| !! | GTGGAGTTGGTAGCTGCTGC+TGG | + | chr4.4:48999959-48999978 | MS.gene97659:CDS | 60.0% |
| AGCTGCTGCTGGACCTTCGG+AGG | + | chr4.4:48999970-48999989 | MS.gene97659:CDS | 65.0% | |
| ! | CGTGCTGCTCCGGATACCTG+TGG | - | chr4.4:49000096-49000115 | None:intergenic | 65.0% |
| GGACATGCAGACGGCGCGTG+GGG | + | chr4.4:49000026-49000045 | MS.gene97659:CDS | 70.0% | |
| GGGACATGCAGACGGCGCGT+GGG | + | chr4.4:49000025-49000044 | MS.gene97659:CDS | 70.0% | |
| TGGGACATGCAGACGGCGCG+TGG | + | chr4.4:49000024-49000043 | MS.gene97659:CDS | 70.0% | |
| AGACGGCGCGTGGGGCTGAC+AGG | + | chr4.4:49000034-49000053 | MS.gene97659:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 48999704 | 49000204 | 48999704 | ID=MS.gene97659 |
| chr4.4 | mRNA | 48999704 | 49000204 | 48999704 | ID=MS.gene97659.t1;Parent=MS.gene97659 |
| chr4.4 | exon | 48999704 | 49000204 | 48999704 | ID=MS.gene97659.t1.exon1;Parent=MS.gene97659.t1 |
| chr4.4 | CDS | 48999704 | 49000204 | 48999704 | ID=cds.MS.gene97659.t1;Parent=MS.gene97659.t1 |
| Gene Sequence |
| Protein sequence |
