Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97836.t1 | XP_024634430.1 | 95.6 | 91 | 3 | 1 | 1 | 91 | 248 | 337 | 2.40E-42 | 181 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97836.t1 | A0A3Q7YD19 | 75.8 | 91 | 21 | 1 | 1 | 91 | 104 | 193 | 2.4e-31 | 144.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051245 | MS.gene97836 | 0.801075 | 1.07E-48 | -1.69E-46 |
| MS.gene057140 | MS.gene97836 | 0.801896 | 7.25E-49 | -1.69E-46 |
| MS.gene058646 | MS.gene97836 | 0.803696 | 3.08E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97836.t1 | MTR_2g017975 | 93.878 | 49 | 3 | 0 | 39 | 87 | 1 | 49 | 1.32e-25 | 94.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAACTTAACAAGGTTATC+TGG | 0.314955 | 5.4:-40543447 | MS.gene97836:CDS |
| CTGATGAGTTGTTTAGGATA+TGG | 0.326241 | 5.4:-40543542 | MS.gene97836:CDS |
| ATTTGCCTGATGAGTTGTTT+AGG | 0.395717 | 5.4:-40543548 | MS.gene97836:CDS |
| TCTTTGCCTTGAAGGTTTGT+AGG | 0.399596 | 5.4:+40543620 | None:intergenic |
| ACTATGAGTCTTTGCCTTGA+AGG | 0.404813 | 5.4:+40543612 | None:intergenic |
| TGCCTTCCTACAAACCTTCA+AGG | 0.421938 | 5.4:-40543626 | MS.gene97836:CDS |
| GAGCTCATCCCTTCACGGTT+AGG | 0.424208 | 5.4:+40543477 | None:intergenic |
| GTTTAGGATATGGGAAGAAA+TGG | 0.432438 | 5.4:-40543532 | MS.gene97836:CDS |
| CATTCGAGCTCATCCCTTCA+CGG | 0.456260 | 5.4:+40543472 | None:intergenic |
| CATATCCTAAACAACTCATC+AGG | 0.463497 | 5.4:+40543543 | None:intergenic |
| TGATGAGTTGTTTAGGATAT+GGG | 0.463980 | 5.4:-40543541 | MS.gene97836:CDS |
| ATAGGAATCATCTTAGAGAT+AGG | 0.479459 | 5.4:-40543398 | MS.gene97836:CDS |
| ATCATCTTAGAGATAGGATG+TGG | 0.498557 | 5.4:-40543392 | MS.gene97836:CDS |
| TGCCTTGAAGGTTTGTAGGA+AGG | 0.520257 | 5.4:+40543624 | None:intergenic |
| TGGTGAAGGTGCAGCTGAGA+TGG | 0.570988 | 5.4:-40543427 | MS.gene97836:CDS |
| CAGCTGAGATGGCAATGGAT+AGG | 0.572746 | 5.4:-40543416 | MS.gene97836:CDS |
| CGAATGTTGAAAACTTAACA+AGG | 0.583345 | 5.4:-40543455 | MS.gene97836:CDS |
| AATGGATGATATAGAACTTG+AGG | 0.590391 | 5.4:-40543514 | MS.gene97836:CDS |
| TTAACAAGGTTATCTGGTGA+AGG | 0.596934 | 5.4:-40543441 | MS.gene97836:CDS |
| TTCACAATTACATACGCAAG+TGG | 0.598213 | 5.4:-40543572 | MS.gene97836:CDS |
| AACGAGGTTCCTAACCGTGA+AGG | 0.603250 | 5.4:-40543486 | MS.gene97836:CDS |
| ACGAGGTTCCTAACCGTGAA+GGG | 0.603814 | 5.4:-40543485 | MS.gene97836:CDS |
| AGAACTTGAGGCGGTGAACG+AGG | 0.616035 | 5.4:-40543502 | MS.gene97836:CDS |
| AGGTGCAGCTGAGATGGCAA+TGG | 0.645965 | 5.4:-40543421 | MS.gene97836:CDS |
| GGATGATATAGAACTTGAGG+CGG | 0.673217 | 5.4:-40543511 | MS.gene97836:CDS |
| TCATCTTAGAGATAGGATGT+GGG | 0.679475 | 5.4:-40543391 | MS.gene97836:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATGGATGATATAGAACTTG+AGG | - | chr5.4:40543487-40543506 | MS.gene97836:CDS | 30.0% | |
| ATAGGAATCATCTTAGAGAT+AGG | - | chr5.4:40543603-40543622 | MS.gene97836:CDS | 30.0% | |
| CGAATGTTGAAAACTTAACA+AGG | - | chr5.4:40543546-40543565 | MS.gene97836:CDS | 30.0% | |
| GAAAACTTAACAAGGTTATC+TGG | - | chr5.4:40543554-40543573 | MS.gene97836:CDS | 30.0% | |
| ! | TGATGAGTTGTTTAGGATAT+GGG | - | chr5.4:40543460-40543479 | MS.gene97836:CDS | 30.0% |
| ATCATCTTAGAGATAGGATG+TGG | - | chr5.4:40543609-40543628 | MS.gene97836:CDS | 35.0% | |
| ATTTGCCTGATGAGTTGTTT+AGG | - | chr5.4:40543453-40543472 | MS.gene97836:CDS | 35.0% | |
| CATATCCTAAACAACTCATC+AGG | + | chr5.4:40543461-40543480 | None:intergenic | 35.0% | |
| TCATCTTAGAGATAGGATGT+GGG | - | chr5.4:40543610-40543629 | MS.gene97836:CDS | 35.0% | |
| TTAACAAGGTTATCTGGTGA+AGG | - | chr5.4:40543560-40543579 | MS.gene97836:CDS | 35.0% | |
| TTCACAATTACATACGCAAG+TGG | - | chr5.4:40543429-40543448 | MS.gene97836:CDS | 35.0% | |
| ! | CTGATGAGTTGTTTAGGATA+TGG | - | chr5.4:40543459-40543478 | MS.gene97836:CDS | 35.0% |
| ! | GTTTAGGATATGGGAAGAAA+TGG | - | chr5.4:40543469-40543488 | MS.gene97836:CDS | 35.0% |
| ACTATGAGTCTTTGCCTTGA+AGG | + | chr5.4:40543392-40543411 | None:intergenic | 40.0% | |
| GGATGATATAGAACTTGAGG+CGG | - | chr5.4:40543490-40543509 | MS.gene97836:CDS | 40.0% | |
| TCTTTGCCTTGAAGGTTTGT+AGG | + | chr5.4:40543384-40543403 | None:intergenic | 40.0% | |
| TGCCTTCCTACAAACCTTCA+AGG | - | chr5.4:40543375-40543394 | MS.gene97836:CDS | 45.0% | |
| TGCCTTGAAGGTTTGTAGGA+AGG | + | chr5.4:40543380-40543399 | None:intergenic | 45.0% | |
| AACGAGGTTCCTAACCGTGA+AGG | - | chr5.4:40543515-40543534 | MS.gene97836:CDS | 50.0% | |
| ACGAGGTTCCTAACCGTGAA+GGG | - | chr5.4:40543516-40543535 | MS.gene97836:CDS | 50.0% | |
| CAGCTGAGATGGCAATGGAT+AGG | - | chr5.4:40543585-40543604 | MS.gene97836:CDS | 50.0% | |
| CATTCGAGCTCATCCCTTCA+CGG | + | chr5.4:40543532-40543551 | None:intergenic | 50.0% | |
| AGAACTTGAGGCGGTGAACG+AGG | - | chr5.4:40543499-40543518 | MS.gene97836:CDS | 55.0% | |
| AGGTGCAGCTGAGATGGCAA+TGG | - | chr5.4:40543580-40543599 | MS.gene97836:CDS | 55.0% | |
| GAGCTCATCCCTTCACGGTT+AGG | + | chr5.4:40543527-40543546 | None:intergenic | 55.0% | |
| ! | TGGTGAAGGTGCAGCTGAGA+TGG | - | chr5.4:40543574-40543593 | MS.gene97836:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 40543374 | 40543649 | 40543374 | ID=MS.gene97836 |
| chr5.4 | mRNA | 40543374 | 40543649 | 40543374 | ID=MS.gene97836.t1;Parent=MS.gene97836 |
| chr5.4 | exon | 40543374 | 40543649 | 40543374 | ID=MS.gene97836.t1.exon1;Parent=MS.gene97836.t1 |
| chr5.4 | CDS | 40543374 | 40543649 | 40543374 | ID=cds.MS.gene97836.t1;Parent=MS.gene97836.t1 |
| Gene Sequence |
| Protein sequence |
