Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98158.t1 | RHN42107.1 | 51.1 | 914 | 214 | 5 | 1 | 685 | 1 | 910 | 2.80E-236 | 828.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98158.t1 | Q9LU39 | 62.4 | 237 | 81 | 3 | 869 | 1105 | 58 | 286 | 6.1e-83 | 310.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98158.t1 | A0A396GU28 | 51.1 | 914 | 214 | 5 | 1 | 685 | 1 | 910 | 2.0e-236 | 828.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053272 | MS.gene98158 | 0.956495 | 2.58E-114 | -1.69E-46 |
| MS.gene05985 | MS.gene98158 | 0.80245 | 5.58E-49 | -1.69E-46 |
| MS.gene060935 | MS.gene98158 | -0.805352 | 1.39E-49 | -1.69E-46 |
| MS.gene060937 | MS.gene98158 | -0.800481 | 1.41E-48 | -1.69E-46 |
| MS.gene061160 | MS.gene98158 | -0.800947 | 1.13E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98158.t1 | MTR_4g006200 | 87.681 | 276 | 30 | 1 | 846 | 1117 | 35 | 310 | 4.01e-170 | 503 |
| MS.gene98158.t1 | MTR_8g070580 | 45.980 | 398 | 157 | 10 | 85 | 481 | 26 | 366 | 3.99e-93 | 308 |
| MS.gene98158.t1 | MTR_4g028910 | 39.818 | 329 | 125 | 5 | 158 | 486 | 148 | 403 | 1.07e-58 | 209 |
| MS.gene98158.t1 | MTR_3g006055 | 70.690 | 116 | 19 | 3 | 964 | 1072 | 1 | 108 | 5.28e-46 | 161 |
| MS.gene98158.t1 | MTR_4g005800 | 88.158 | 76 | 9 | 0 | 1001 | 1076 | 48 | 123 | 1.05e-42 | 152 |
| MS.gene98158.t1 | MTR_3g049490 | 50.500 | 200 | 37 | 6 | 602 | 791 | 11 | 158 | 1.37e-41 | 151 |
| MS.gene98158.t1 | MTR_5g081480 | 26.389 | 432 | 257 | 9 | 372 | 779 | 468 | 862 | 9.16e-40 | 160 |
| MS.gene98158.t1 | MTR_6g462630 | 36.842 | 114 | 71 | 1 | 547 | 660 | 39 | 151 | 1.03e-19 | 89.4 |
| MS.gene98158.t1 | MTR_2g048835 | 32.143 | 168 | 92 | 1 | 493 | 660 | 279 | 424 | 1.06e-19 | 94.4 |
| MS.gene98158.t1 | MTR_6g022470 | 30.088 | 226 | 132 | 6 | 558 | 763 | 29 | 248 | 3.18e-18 | 86.7 |
| MS.gene98158.t1 | MTR_7g090610 | 26.562 | 256 | 156 | 6 | 485 | 730 | 36 | 269 | 1.57e-17 | 86.3 |
| MS.gene98158.t1 | MTR_6g059380 | 38.281 | 128 | 71 | 4 | 5 | 126 | 391 | 516 | 7.88e-16 | 82.4 |
| MS.gene98158.t1 | MTR_1g046450 | 75.000 | 44 | 11 | 0 | 440 | 483 | 1 | 44 | 2.20e-15 | 75.1 |
| MS.gene98158.t1 | MTR_2g020400 | 32.174 | 115 | 77 | 1 | 554 | 668 | 1 | 114 | 4.98e-14 | 72.0 |
| MS.gene98158.t1 | MTR_3g436320 | 47.143 | 70 | 35 | 1 | 591 | 660 | 56 | 123 | 3.15e-13 | 69.3 |
| MS.gene98158.t1 | MTR_8g040660 | 27.108 | 166 | 78 | 2 | 493 | 658 | 13 | 135 | 6.52e-13 | 70.9 |
| MS.gene98158.t1 | MTR_2g090645 | 38.372 | 86 | 53 | 0 | 590 | 675 | 1 | 86 | 9.32e-12 | 66.6 |
| MS.gene98158.t1 | MTR_2g461790 | 30.818 | 159 | 91 | 5 | 571 | 715 | 9 | 162 | 2.40e-11 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98158.t1 | AT3G21200 | 61.925 | 239 | 83 | 3 | 869 | 1107 | 58 | 288 | 3.49e-99 | 317 |
| MS.gene98158.t1 | AT5G15685 | 25.253 | 198 | 140 | 3 | 285 | 480 | 148 | 339 | 1.53e-17 | 87.8 |
| MS.gene98158.t1 | AT3G05850 | 24.790 | 238 | 160 | 5 | 251 | 476 | 134 | 364 | 5.17e-16 | 84.0 |
| MS.gene98158.t1 | AT3G03890 | 27.907 | 258 | 154 | 12 | 864 | 1103 | 75 | 318 | 4.89e-11 | 65.9 |
Find 271 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAGCTGCTGCTGCAGCTTC+TGG | 0.126399 | 4.1:-88993643 | MS.gene98158:CDS |
| CAAGCTGGTGCTGCAGCTTC+TGG | 0.137923 | 4.1:-88993676 | MS.gene98158:CDS |
| GCCTTTCTTGCTTCCTGATT+TGG | 0.158288 | 4.1:+88993935 | None:intergenic |
| TGAAGTTTCAAGAACAATTA+TGG | 0.206636 | 4.1:-88989639 | MS.gene98158:CDS |
| CCTTTCTTGCTTCCTGATTT+GGG | 0.213044 | 4.1:+88993936 | None:intergenic |
| CTACTAAATCTAATATAATA+TGG | 0.218198 | 4.1:+88995647 | None:intergenic |
| AGTAATGTCTTCCATATTAT+TGG | 0.225630 | 4.1:+88987792 | None:intergenic |
| AAGGGTGAAATACATGAATA+TGG | 0.226596 | 4.1:-88996484 | MS.gene98158:CDS |
| GAACATGTGCATGCAGAAAA+TGG | 0.233815 | 4.1:-88994306 | MS.gene98158:CDS |
| TCTCTATCACCACACCAAAT+TGG | 0.237549 | 4.1:+88994388 | None:intergenic |
| ATCTTCAATTTCAACATCTT+TGG | 0.238086 | 4.1:+88996255 | None:intergenic |
| CAAACGCATACTTGCCATTT+CGG | 0.254554 | 4.1:-88986441 | MS.gene98158:CDS |
| CCCTGTTGTCGCTTACAATA+AGG | 0.268691 | 4.1:-88996503 | MS.gene98158:CDS |
| AGGCATCCCAGGCGCCCTTC+TGG | 0.275672 | 4.1:+88993989 | None:intergenic |
| CAGCGGTTGGGTTCAAAGTT+TGG | 0.289733 | 4.1:+88996011 | None:intergenic |
| TTGACATAGTTGATGGTCTA+AGG | 0.290889 | 4.1:-88996357 | MS.gene98158:CDS |
| CTATGTTGATTTGGATTTCC+AGG | 0.294434 | 4.1:-88987755 | MS.gene98158:intron |
| CTCTATCACCACACCAAATT+GGG | 0.298718 | 4.1:+88994389 | None:intergenic |
| TGCCACTATGCTCTAATATA+TGG | 0.300262 | 4.1:+88986413 | None:intergenic |
| CCTGATCCGGATTGGATTCA+TGG | 0.302049 | 4.1:-88990086 | MS.gene98158:CDS |
| ACACCAACTATAGATTGATT+AGG | 0.309452 | 4.1:+88995971 | None:intergenic |
| GAAATGAAGTGGTTCGAAAA+TGG | 0.310193 | 4.1:-88997065 | MS.gene98158:CDS |
| TCACAGAAGCAAAGATGATA+TGG | 0.313832 | 4.1:-88987418 | MS.gene98158:CDS |
| TGTATATTATTGCTGTAGAT+AGG | 0.315510 | 4.1:-88988076 | MS.gene98158:CDS |
| GCTGCACTTGGATGACTTCC+AGG | 0.317388 | 4.1:-88988051 | MS.gene98158:CDS |
| TGAAATTAACACCAATAATA+TGG | 0.323447 | 4.1:-88987803 | MS.gene98158:CDS |
| ATTGGTTCTGTAGCAACTTC+TGG | 0.323493 | 4.1:+88994026 | None:intergenic |
| TCATCTTCTAATTCACATTC+AGG | 0.329003 | 4.1:+88996112 | None:intergenic |
| GGTACAACTGCTGCTGCATT+TGG | 0.329342 | 4.1:-88993571 | MS.gene98158:CDS |
| TCTTCCACACCAGTCACTTC+AGG | 0.330956 | 4.1:+88995932 | None:intergenic |
| ACTACATCTACCAACACTTC+TGG | 0.332900 | 4.1:-88993493 | MS.gene98158:CDS |
| CATATTATATTAGATTTAGT+AGG | 0.334674 | 4.1:-88995646 | MS.gene98158:CDS |
| CCCTTATTGTAAGCGACAAC+AGG | 0.337317 | 4.1:+88996502 | None:intergenic |
| CTTCAAATGGCCCAAGACTA+TGG | 0.345755 | 4.1:-88994049 | MS.gene98158:CDS |
| TTCTGCATCTAACAGTTTCC+TGG | 0.346526 | 4.1:+88993904 | None:intergenic |
| GTTCAGAATAATTGCCGAAT+TGG | 0.347040 | 4.1:+88986471 | None:intergenic |
| TCCACAATCTTCCTTATCAT+TGG | 0.351354 | 4.1:+88994442 | None:intergenic |
| CTCTTCTTTCAGAGCCTTAA+GGG | 0.351494 | 4.1:+88995484 | None:intergenic |
| CCTCCTGCCAGAAGGGCGCC+TGG | 0.351676 | 4.1:-88993996 | MS.gene98158:CDS |
| CAAATAGGATAGTTAAGTTA+AGG | 0.352962 | 4.1:-88994498 | MS.gene98158:intron |
| CTAATGGTTGGACCCCAATT+TGG | 0.356310 | 4.1:-88994402 | MS.gene98158:CDS |
| AAAGATGCTGTGAAGGAGTA+TGG | 0.358540 | 4.1:-88995746 | MS.gene98158:CDS |
| ACTCTTCTTTCAGAGCCTTA+AGG | 0.359227 | 4.1:+88995483 | None:intergenic |
| AATGCTACAACAAACACTTC+TGG | 0.360211 | 4.1:-88993424 | MS.gene98158:CDS |
| AAAAGGAATAGGTTTAAGTT+AGG | 0.361279 | 4.1:-88995830 | MS.gene98158:CDS |
| GCTGCAGCAGCAGCTTGTGC+AGG | 0.362322 | 4.1:+88993649 | None:intergenic |
| CACCAACTATAGATTGATTA+GGG | 0.364039 | 4.1:+88995972 | None:intergenic |
| CTGGCAGGAGGTGGGAGTAT+TGG | 0.365326 | 4.1:+88994008 | None:intergenic |
| TTGTAATGTCTATGTTGATT+TGG | 0.370501 | 4.1:-88987764 | MS.gene98158:CDS |
| TAAGGTTGTTGAAAACTGAT+AGG | 0.374747 | 4.1:-88996339 | MS.gene98158:CDS |
| CAGACGCATACTTGCCAATT+CGG | 0.377781 | 4.1:-88986485 | MS.gene98158:CDS |
| CACAGCGGCTTGTTTCTTTG+TGG | 0.379289 | 4.1:-88988136 | MS.gene98158:intron |
| TGAATCTTAACACTGCTTCC+AGG | 0.380929 | 4.1:+88995320 | None:intergenic |
| ACTTTCAACGTTAACCCAAC+AGG | 0.382511 | 4.1:-88989597 | MS.gene98158:CDS |
| TGTGGTGATGAGTTCTTATC+AGG | 0.384320 | 4.1:-88995444 | MS.gene98158:CDS |
| ATTTAACTGTATGTCACAAT+TGG | 0.386834 | 4.1:-88987285 | MS.gene98158:CDS |
| GCAACTTCTGGCCATAGTCT+TGG | 0.388664 | 4.1:+88994038 | None:intergenic |
| CTAGAAAGAGGAAAAGGAAT+AGG | 0.389149 | 4.1:-88995841 | MS.gene98158:CDS |
| AGAGAAGTCACAGATGAAAA+AGG | 0.391076 | 4.1:-88987320 | MS.gene98158:CDS |
| TTCGTTGTTGATTCTGAAGA+TGG | 0.392454 | 4.1:-88989548 | MS.gene98158:CDS |
| GATGATATGGGTAGACCGTT+TGG | 0.393043 | 4.1:-88987405 | MS.gene98158:CDS |
| AATATATGGAATTGCCGAAA+TGG | 0.396712 | 4.1:+88986427 | None:intergenic |
| CAATGAACCTGTTGTATTTG+AGG | 0.397088 | 4.1:-88995801 | MS.gene98158:CDS |
| CCCTCATCACCAATGTAATC+AGG | 0.399810 | 4.1:+88996142 | None:intergenic |
| GACAAAGTGATCAGAGAGAT+TGG | 0.401513 | 4.1:-88996421 | MS.gene98158:CDS |
| AGGGGTGCTGCTTATGGGTG+TGG | 0.402493 | 4.1:+88990057 | None:intergenic |
| GGCGCCCTTCTGGCAGGAGG+TGG | 0.402770 | 4.1:+88993999 | None:intergenic |
| AGTGTTAACCACAAAATTGT+TGG | 0.403004 | 4.1:-88995549 | MS.gene98158:CDS |
| GGCGCCTGGGATGCCTAAAA+AGG | 0.403199 | 4.1:-88993982 | MS.gene98158:CDS |
| TGCATGCACATGTTCTGTCT+TGG | 0.405546 | 4.1:+88994314 | None:intergenic |
| CATTTGATCCTTCCTTCAAA+TGG | 0.406789 | 4.1:-88994062 | MS.gene98158:CDS |
| ATGGGTAGACCGTTTGGGCT+TGG | 0.410130 | 4.1:-88987399 | MS.gene98158:CDS |
| TAAGGTCAAATTAATAGATC+TGG | 0.416724 | 4.1:-88996443 | MS.gene98158:CDS |
| ATGATATGGGTAGACCGTTT+GGG | 0.419361 | 4.1:-88987404 | MS.gene98158:CDS |
| AAACTGATAGGGATGTTGTT+AGG | 0.427043 | 4.1:-88996327 | MS.gene98158:CDS |
| TCCTAACAACGGTTTATTTG+AGG | 0.427212 | 4.1:-88987360 | MS.gene98158:CDS |
| GAACAATTATGGATTTAGCT+AGG | 0.431943 | 4.1:-88989628 | MS.gene98158:CDS |
| TACTAAATCTAATATAATAT+GGG | 0.433913 | 4.1:+88995648 | None:intergenic |
| CATAGTCTTGGGCCATTTGA+AGG | 0.434602 | 4.1:+88994050 | None:intergenic |
| CAACTTCTGGCCATAGTCTT+GGG | 0.436093 | 4.1:+88994039 | None:intergenic |
| AGTTAAGGGACTACATGTTA+AGG | 0.436124 | 4.1:-88994483 | MS.gene98158:CDS |
| CCTGTTGTCGCTTACAATAA+GGG | 0.439672 | 4.1:-88996502 | MS.gene98158:CDS |
| ACAAAGTGATCAGAGAGATT+GGG | 0.440359 | 4.1:-88996420 | MS.gene98158:CDS |
| AATCCAATCCGGATCAGGTT+TGG | 0.440523 | 4.1:+88990091 | None:intergenic |
| AGTGCTTTGGAAACCATTCA+TGG | 0.440893 | 4.1:-88995404 | MS.gene98158:CDS |
| AAATAGGATAGTTAAGTTAA+GGG | 0.442593 | 4.1:-88994497 | MS.gene98158:intron |
| TCTTTGTGGACGAAGAGGTT+TGG | 0.443453 | 4.1:-88988122 | MS.gene98158:CDS |
| GAAATAACATAATGAGTTTG+AGG | 0.443897 | 4.1:+88995617 | None:intergenic |
| ACTGTATGTCACAATTGGCT+TGG | 0.444223 | 4.1:-88987280 | MS.gene98158:CDS |
| AACATTACCTTGTTCCCTCC+AGG | 0.447840 | 4.1:+88993813 | None:intergenic |
| ATTATGTGGCACACTGGTAT+AGG | 0.449508 | 4.1:-88994216 | MS.gene98158:intron |
| ACCCAACAGGGTTATCCTCT+TGG | 0.450020 | 4.1:-88989584 | MS.gene98158:CDS |
| CTGTATGTCACAATTGGCTT+GGG | 0.450082 | 4.1:-88987279 | MS.gene98158:CDS |
| GAGAATGATGAGCCCAAATC+AGG | 0.451458 | 4.1:-88993948 | MS.gene98158:CDS |
| GTTGTAGCATTTGATACAGC+AGG | 0.452285 | 4.1:+88993436 | None:intergenic |
| GTTGGTCAAATATTTGCAAA+TGG | 0.453153 | 4.1:-88995779 | MS.gene98158:CDS |
| ATCCCAGGCGCCCTTCTGGC+AGG | 0.454491 | 4.1:+88993993 | None:intergenic |
| CCCAAATCAGGAAGCAAGAA+AGG | 0.454801 | 4.1:-88993936 | MS.gene98158:CDS |
| AGAGCCTTAAGGGTCATAGA+AGG | 0.457924 | 4.1:+88995494 | None:intergenic |
| CCATGAATCCAATCCGGATC+AGG | 0.459717 | 4.1:+88990086 | None:intergenic |
| AGGACCACAGTTTAAAGATA+AGG | 0.462010 | 4.1:-88996555 | MS.gene98158:CDS |
| TGACCAACCTCAAATACAAC+AGG | 0.462785 | 4.1:+88995794 | None:intergenic |
| AACAATTATGGATTTAGCTA+GGG | 0.462844 | 4.1:-88989627 | MS.gene98158:CDS |
| GACTACATGTTAAGGTTTCA+AGG | 0.462947 | 4.1:-88994475 | MS.gene98158:CDS |
| AGGGTGAAATACATGAATAT+GGG | 0.463361 | 4.1:-88996483 | MS.gene98158:CDS |
| TAGAACAGCCAAACCTGATC+CGG | 0.464717 | 4.1:-88990099 | MS.gene98158:CDS |
| GATGAGGATGGAAGTGTTGA+TGG | 0.466640 | 4.1:-88996094 | MS.gene98158:CDS |
| TTCATCACTAGCACCATGAA+TGG | 0.466862 | 4.1:+88995391 | None:intergenic |
| CTTGAAACTTCAGATGGAAA+CGG | 0.466933 | 4.1:+88989650 | None:intergenic |
| ACTCCTCAGTGTACACTTCA+AGG | 0.467071 | 4.1:-88989310 | MS.gene98158:CDS |
| ACAGTCGAAGGTGACGAGTT+CGG | 0.467474 | 4.1:-88997042 | MS.gene98158:CDS |
| TGTAGATAGGGTGCTGCACT+TGG | 0.473744 | 4.1:-88988063 | MS.gene98158:CDS |
| AACATGTGCATGCAGAAAAT+GGG | 0.478387 | 4.1:-88994305 | MS.gene98158:CDS |
| GGGACATTTGTCCAATGATA+AGG | 0.478437 | 4.1:-88994453 | MS.gene98158:CDS |
| CTCCTGCCAGAAGGGCGCCT+GGG | 0.480568 | 4.1:-88993995 | MS.gene98158:CDS |
| GGGGTGCTGCTTATGGGTGT+GGG | 0.481917 | 4.1:+88990058 | None:intergenic |
| CAGTGTGCCACATAATCATC+AGG | 0.482430 | 4.1:+88994223 | None:intergenic |
| ATCAGAGAGATTGGGGTGGT+AGG | 0.486789 | 4.1:-88996412 | MS.gene98158:CDS |
| CTTCCACACCAGTCACTTCA+GGG | 0.487089 | 4.1:+88995933 | None:intergenic |
| GGGGCAGAATTGTTGTTGAC+AGG | 0.487111 | 4.1:+88993457 | None:intergenic |
| AAAGATAAGGTTTCACTACA+AGG | 0.488463 | 4.1:-88996542 | MS.gene98158:CDS |
| CAGGGTTATCCTCTTGGTGT+TGG | 0.489252 | 4.1:-88989578 | MS.gene98158:CDS |
| TGTCTTTCCATCACATGTCC+TGG | 0.489614 | 4.1:+88993857 | None:intergenic |
| ATTATGGATTTAGCTAGGGT+TGG | 0.490791 | 4.1:-88989623 | MS.gene98158:CDS |
| TGCAGAAGGTGTAAAGAGCT+TGG | 0.492061 | 4.1:-88993888 | MS.gene98158:CDS |
| ACCTCAAATAAACCGTTGTT+AGG | 0.493427 | 4.1:+88987359 | None:intergenic |
| CAGCCAAACCTGATCCGGAT+TGG | 0.493762 | 4.1:-88990094 | MS.gene98158:CDS |
| GATCATAGTTGCCATCCAAC+TGG | 0.494588 | 4.1:-88995581 | MS.gene98158:CDS |
| ATTGTTCTTGAAACTTCAGA+TGG | 0.496689 | 4.1:+88989644 | None:intergenic |
| AGTTCAGCGAAGAAAGTAGC+AGG | 0.500612 | 4.1:-88996996 | MS.gene98158:CDS |
| AACCCTAATCAATCTATAGT+TGG | 0.503822 | 4.1:-88995974 | MS.gene98158:CDS |
| ACATATCTAGAAAGAGGAAA+AGG | 0.503961 | 4.1:-88995847 | MS.gene98158:CDS |
| AAGTTGTGGTATATCTGTCC+AGG | 0.504666 | 4.1:-88996382 | MS.gene98158:CDS |
| ATTATGATGAAGGGTGGGAT+TGG | 0.504675 | 4.1:-88996051 | MS.gene98158:CDS |
| TAGTTGGTGTTGAAGTATCT+AGG | 0.505809 | 4.1:-88995958 | MS.gene98158:CDS |
| TCAGGATCCGGCTTTGATCA+AGG | 0.506394 | 4.1:-88989272 | MS.gene98158:intron |
| CAAGAAAGGCAAGAGAAACC+AGG | 0.506435 | 4.1:-88993922 | MS.gene98158:CDS |
| CTGACTGACTAACAGCGGTT+GGG | 0.509248 | 4.1:+88995999 | None:intergenic |
| ACTGACTGACTAACAGCGGT+TGG | 0.509524 | 4.1:+88995998 | None:intergenic |
| AACACTTCTGGACTTGCTAA+AGG | 0.511145 | 4.1:-88993481 | MS.gene98158:CDS |
| GAAGGTGTATGCTTGAGTAT+AGG | 0.511740 | 4.1:+88995512 | None:intergenic |
| TACACCTTCTATGACCCTTA+AGG | 0.512591 | 4.1:-88995498 | MS.gene98158:CDS |
| GTTAGTTAAAGATGCTGTGA+AGG | 0.513329 | 4.1:-88995753 | MS.gene98158:CDS |
| CCTGATTACATTGGTGATGA+GGG | 0.516897 | 4.1:-88996142 | MS.gene98158:CDS |
| AGAATGACATATCTAGAAAG+AGG | 0.517911 | 4.1:-88995853 | MS.gene98158:CDS |
| GTATATTATTGCTGTAGATA+GGG | 0.518730 | 4.1:-88988075 | MS.gene98158:CDS |
| AAGAGCTTGGACATAATGCC+AGG | 0.520612 | 4.1:-88993875 | MS.gene98158:CDS |
| GAGTTCGGAGAAGACGAGTT+CGG | 0.521007 | 4.1:-88997027 | MS.gene98158:CDS |
| GATGATAGTGATTATGATGA+AGG | 0.521069 | 4.1:-88996061 | MS.gene98158:CDS |
| TTTCATCTGTGACTTCTCTA+GGG | 0.522593 | 4.1:+88987324 | None:intergenic |
| AGTAGCAGGGACGACGAAGA+AGG | 0.523045 | 4.1:-88996982 | MS.gene98158:CDS |
| AGGAAGGATCAAATGGTCAT+AGG | 0.523349 | 4.1:+88994070 | None:intergenic |
| CCATCAACTATGTCAAAACC+TGG | 0.523532 | 4.1:+88996364 | None:intergenic |
| CAAGCAACTGCATGAGCACA+TGG | 0.523753 | 4.1:+88994268 | None:intergenic |
| ACCAAGAGGATAACCCTGTT+GGG | 0.525860 | 4.1:+88989583 | None:intergenic |
| GGCACAGAAATGTTGGCAGC+AGG | 0.526554 | 4.1:+88993523 | None:intergenic |
| GAAGGAAACATGCTTAATGT+AGG | 0.527631 | 4.1:-88996927 | MS.gene98158:CDS |
| TTTAGCAGGCACAGAAATGT+TGG | 0.528090 | 4.1:+88993516 | None:intergenic |
| CATACAGTTAAATGTTGACT+TGG | 0.529692 | 4.1:+88987295 | None:intergenic |
| GAGTTGCTCAGAAGTAATCC+TGG | 0.531135 | 4.1:-88995338 | MS.gene98158:CDS |
| ATTGTGGTGAAGTGCTCAAA+AGG | 0.531408 | 4.1:-88995674 | MS.gene98158:CDS |
| GTTGGATGGCAACTATGATC+AGG | 0.531452 | 4.1:+88995584 | None:intergenic |
| GTTGGGTTCAAAGTTTGGCT+AGG | 0.532393 | 4.1:+88996016 | None:intergenic |
| GCGCCCTTCTGGCAGGAGGT+GGG | 0.533123 | 4.1:+88994000 | None:intergenic |
| ATATTATATTAGATTTAGTA+GGG | 0.533632 | 4.1:-88995645 | MS.gene98158:CDS |
| ATGTTGAAATTGAAGATAGC+AGG | 0.534129 | 4.1:-88996249 | MS.gene98158:CDS |
| TCCAATGATAAGGAAGATTG+TGG | 0.534293 | 4.1:-88994443 | MS.gene98158:CDS |
| TCTAGGAACCCTGAAGTGAC+TGG | 0.534331 | 4.1:-88995941 | MS.gene98158:CDS |
| ACCTTGACATGAAGTGAAGA+GGG | 0.536966 | 4.1:+88989464 | None:intergenic |
| GGGTGAGTGGGACATTGATA+AGG | 0.543145 | 4.1:-88996461 | MS.gene98158:CDS |
| TCCTGATTACATTGGTGATG+AGG | 0.543291 | 4.1:-88996143 | MS.gene98158:CDS |
| CTATCATCACACTCATCAGC+TGG | 0.544334 | 4.1:+88996178 | None:intergenic |
| AGGTTCTTGTAGTTGACAAA+GGG | 0.544357 | 4.1:-88996207 | MS.gene98158:CDS |
| GTCGATCCATGAATCCAATC+CGG | 0.545314 | 4.1:+88990080 | None:intergenic |
| CATTTGAAGGAAGGATCAAA+TGG | 0.546048 | 4.1:+88994063 | None:intergenic |
| CACCAAGAGGATAACCCTGT+TGG | 0.548682 | 4.1:+88989582 | None:intergenic |
| TACTCCCACCTCCTGCCAGA+AGG | 0.551800 | 4.1:-88994004 | MS.gene98158:CDS |
| GAGGTTCTTGTAGTTGACAA+AGG | 0.551846 | 4.1:-88996208 | MS.gene98158:CDS |
| CACAGAAGCAAAGATGATAT+GGG | 0.552316 | 4.1:-88987417 | MS.gene98158:CDS |
| TCACTCACCTTGATCAAAGC+CGG | 0.553576 | 4.1:+88989265 | None:intergenic |
| GAACCTGTTGTATTTGAGGT+TGG | 0.556736 | 4.1:-88995797 | MS.gene98158:CDS |
| GTCTTGGGCCATTTGAAGGA+AGG | 0.557978 | 4.1:+88994054 | None:intergenic |
| AATTCTCAGCAAACTCTCGA+AGG | 0.558854 | 4.1:+88987833 | None:intergenic |
| AAGAAGAGTGCAAGAGTAGG+TGG | 0.559370 | 4.1:-88995469 | MS.gene98158:CDS |
| ACTCCCACCTCCTGCCAGAA+GGG | 0.559475 | 4.1:-88994003 | MS.gene98158:CDS |
| AAGGTTGTTGAAAACTGATA+GGG | 0.559616 | 4.1:-88996338 | MS.gene98158:CDS |
| GGGTGAAATACATGAATATG+GGG | 0.559653 | 4.1:-88996482 | MS.gene98158:CDS |
| CATAATACTTCTGCACAAGC+TGG | 0.561212 | 4.1:-88993691 | MS.gene98158:CDS |
| TTTAGCAAGTCCAGAAGTGT+TGG | 0.561346 | 4.1:+88993483 | None:intergenic |
| AACACTCTAATCTTACCAGT+TGG | 0.562384 | 4.1:+88995566 | None:intergenic |
| AGAGGTGGCAACCATGTTGT+TGG | 0.564845 | 4.1:-88993394 | MS.gene98158:intron |
| TTGTAGCATTTGATACAGCA+GGG | 0.566316 | 4.1:+88993437 | None:intergenic |
| GCAGCTTCTGGTGCTGCAAG+AGG | 0.566437 | 4.1:-88993631 | MS.gene98158:CDS |
| AGATAGCAGGTATGAAAGTG+AGG | 0.567901 | 4.1:-88996236 | MS.gene98158:CDS |
| GAAATCCAAACGAAATGAAG+TGG | 0.569922 | 4.1:-88997076 | None:intergenic |
| ATTCTCAGCAAACTCTCGAA+GGG | 0.571271 | 4.1:+88987834 | None:intergenic |
| CTAGCAGCAACAACAGATGA+AGG | 0.572178 | 4.1:-88996945 | MS.gene98158:CDS |
| CTTTCAACGTTAACCCAACA+GGG | 0.573683 | 4.1:-88989596 | MS.gene98158:CDS |
| GTGAATGTTATTATCAGTGG+TGG | 0.576165 | 4.1:+88989490 | None:intergenic |
| TGCAGAAAATGGGATTTAAG+TGG | 0.577735 | 4.1:-88994295 | MS.gene98158:CDS |
| TATTGCACCTGGAGGGAACA+AGG | 0.577843 | 4.1:-88993820 | MS.gene98158:intron |
| CAAAGTGATCAGAGAGATTG+GGG | 0.579272 | 4.1:-88996419 | MS.gene98158:CDS |
| CTCTAATCTTACCAGTTGGA+TGG | 0.580214 | 4.1:+88995570 | None:intergenic |
| AGGAACGCTCACGAAACCTC+AGG | 0.583087 | 4.1:-88989290 | MS.gene98158:CDS |
| AACGCATATCCAAGCCCAAA+CGG | 0.583766 | 4.1:+88987390 | None:intergenic |
| AATGAGCATAAGAATTCAGT+AGG | 0.584058 | 4.1:-88996298 | MS.gene98158:CDS |
| ACTACATGTTAAGGTTTCAA+GGG | 0.584653 | 4.1:-88994474 | MS.gene98158:CDS |
| AGGACATGTGATGGAAAGAC+AGG | 0.584761 | 4.1:-88993855 | MS.gene98158:CDS |
| AAGATAAGGTTTCACTACAA+GGG | 0.587195 | 4.1:-88996541 | MS.gene98158:CDS |
| TCTGTGACTTCTCTAGGGAA+AGG | 0.588455 | 4.1:+88987329 | None:intergenic |
| AGCTAATCCTGATGATTATG+TGG | 0.588957 | 4.1:-88994230 | MS.gene98158:CDS |
| TACCTTGACATGAAGTGAAG+AGG | 0.592658 | 4.1:+88989463 | None:intergenic |
| CCAGGCGCCCTTCTGGCAGG+AGG | 0.595545 | 4.1:+88993996 | None:intergenic |
| CCCCTCTTCACTTCATGTCA+AGG | 0.595937 | 4.1:-88989465 | MS.gene98158:intron |
| GTGCAAGAGTAGGTGGAATG+TGG | 0.596265 | 4.1:-88995462 | MS.gene98158:CDS |
| TTCCATATATTAGAGCATAG+TGG | 0.597139 | 4.1:-88986415 | None:intergenic |
| GGTGCTAGTGATGAACAATA+TGG | 0.598076 | 4.1:-88995383 | MS.gene98158:CDS |
| TTGTTTCTTTGTGGACGAAG+AGG | 0.598679 | 4.1:-88988127 | MS.gene98158:CDS |
| GTATGAAAGTGAGGTTGAAG+AGG | 0.599386 | 4.1:-88996227 | MS.gene98158:CDS |
| ATGATAGTGATTATGATGAA+GGG | 0.599674 | 4.1:-88996060 | MS.gene98158:CDS |
| TATGTCACAATTGGCTTGGG+AGG | 0.603045 | 4.1:-88987276 | MS.gene98158:intron |
| GCTCACGAAACCTCAGGATC+CGG | 0.604789 | 4.1:-88989284 | MS.gene98158:CDS |
| AACACTTCTGGAACAATGAG+AGG | 0.604863 | 4.1:-88993412 | MS.gene98158:CDS |
| TTGATCAAAGCCGGATCCTG+AGG | 0.605727 | 4.1:+88989274 | None:intergenic |
| GTGGTGATAGAGACTCCTCT+AGG | 0.607335 | 4.1:-88994378 | MS.gene98158:CDS |
| TGTGAATTAGAAGATGAGGA+TGG | 0.609187 | 4.1:-88996106 | MS.gene98158:CDS |
| GGTGTGAATGTTATTATCAG+TGG | 0.609297 | 4.1:+88989487 | None:intergenic |
| TGAAAGAAGAGTGCAAGAGT+AGG | 0.610275 | 4.1:-88995472 | MS.gene98158:CDS |
| CATAATGCCAGGACATGTGA+TGG | 0.611273 | 4.1:-88993864 | MS.gene98158:CDS |
| ATAGTGATTATGATGAAGGG+TGG | 0.613625 | 4.1:-88996057 | MS.gene98158:CDS |
| AGTTGAGATTAACTACTGCA+TGG | 0.614060 | 4.1:-88990146 | MS.gene98158:CDS |
| GCTGACAGAAGTATTGCACC+TGG | 0.625515 | 4.1:-88993831 | MS.gene98158:CDS |
| GAAACCTTATCTTTAAACTG+TGG | 0.628105 | 4.1:+88996551 | None:intergenic |
| GTTCGAAAATGGACAGTCGA+AGG | 0.629519 | 4.1:-88997054 | MS.gene98158:CDS |
| GAGACAGATCCTGATTACAT+TGG | 0.630730 | 4.1:-88996151 | MS.gene98158:CDS |
| TTCTCAGCAAACTCTCGAAG+GGG | 0.632254 | 4.1:+88987835 | None:intergenic |
| GAACCCTGAAGTGACTGGTG+TGG | 0.634357 | 4.1:-88995936 | MS.gene98158:CDS |
| CTACATGTTAAGGTTTCAAG+GGG | 0.636455 | 4.1:-88994473 | MS.gene98158:CDS |
| AGGAAACTGTTAGATGCAGA+AGG | 0.636560 | 4.1:-88993902 | MS.gene98158:CDS |
| CTGATGATTATGTGGCACAC+TGG | 0.637174 | 4.1:-88994222 | MS.gene98158:CDS |
| CCTTATTGTAAGCGACAACA+GGG | 0.640868 | 4.1:+88996503 | None:intergenic |
| AATCGAACACCAACACCAAG+AGG | 0.641303 | 4.1:+88989569 | None:intergenic |
| CTTCTCTAGGGAAAGGAATG+CGG | 0.641717 | 4.1:+88987336 | None:intergenic |
| TCTCAGCAAACTCTCGAAGG+GGG | 0.641945 | 4.1:+88987836 | None:intergenic |
| AAGCTACCTCACCAACAACA+TGG | 0.643234 | 4.1:+88993383 | None:intergenic |
| TGAATGTGAATTAGAAGATG+AGG | 0.645096 | 4.1:-88996110 | MS.gene98158:CDS |
| TGGTAGGAGACTACAAGTTG+TGG | 0.651977 | 4.1:-88996396 | MS.gene98158:CDS |
| TCTATCACCACACCAAATTG+GGG | 0.653462 | 4.1:+88994390 | None:intergenic |
| TGAATGTTATTATCAGTGGT+GGG | 0.656145 | 4.1:+88989491 | None:intergenic |
| TTTGATTTGACGGTCAACAA+CGG | 0.657465 | 4.1:+88990201 | None:intergenic |
| ACAGAAGTATTGCACCTGGA+GGG | 0.658479 | 4.1:-88993827 | MS.gene98158:CDS |
| ATAACATAATGAGTTTGAGG+AGG | 0.661447 | 4.1:+88995620 | None:intergenic |
| TAGTGATTATGATGAAGGGT+GGG | 0.665329 | 4.1:-88996056 | MS.gene98158:CDS |
| GGTGAAATACATGAATATGG+GGG | 0.666500 | 4.1:-88996481 | MS.gene98158:CDS |
| GTTCAGCGAAGAAAGTAGCA+GGG | 0.668407 | 4.1:-88996995 | MS.gene98158:CDS |
| GGTTGGACCCCAATTTGGTG+TGG | 0.679052 | 4.1:-88994397 | MS.gene98158:CDS |
| ATCAGTAACTGAAAACCTAG+AGG | 0.679127 | 4.1:+88994363 | None:intergenic |
| CTTGACATGAAGTGAAGAGG+GGG | 0.679817 | 4.1:+88989466 | None:intergenic |
| AAGCAACTGCATGAGCACAT+GGG | 0.680933 | 4.1:+88994269 | None:intergenic |
| ACTTCTGGAACAATGAGAGG+TGG | 0.685190 | 4.1:-88993409 | MS.gene98158:CDS |
| GACAGAAGTATTGCACCTGG+AGG | 0.687021 | 4.1:-88993828 | MS.gene98158:CDS |
| TCATCTAAGAAACTATGCTG+AGG | 0.687339 | 4.1:-88995360 | MS.gene98158:CDS |
| TGATGAGTGTGATGATAGTG+AGG | 0.696290 | 4.1:-88996173 | MS.gene98158:CDS |
| CCTTGACATGAAGTGAAGAG+GGG | 0.702259 | 4.1:+88989465 | None:intergenic |
| TGTAGCATTTGATACAGCAG+GGG | 0.716619 | 4.1:+88993438 | None:intergenic |
| GACGACGAAGAAGGTTACAG+AGG | 0.724562 | 4.1:-88996973 | MS.gene98158:CDS |
| ATTTAACACAGTTTACTGTG+TGG | 0.732996 | 4.1:-88990233 | MS.gene98158:intron |
| GCACTTGGATGACTTCCAGG+AGG | 0.736814 | 4.1:-88988048 | MS.gene98158:intron |
| GTTAACTGACTGACTAACAG+CGG | 0.742713 | 4.1:+88995994 | None:intergenic |
| AGTGATCAGAGAGATTGGGG+TGG | 0.753650 | 4.1:-88996416 | MS.gene98158:CDS |
| GTTCCTTGAAGTGTACACTG+AGG | 0.794003 | 4.1:+88989307 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 88986418 | 88997084 | 88986418 | ID=MS.gene98158 |
| chr4.1 | mRNA | 88986418 | 88997084 | 88986418 | ID=MS.gene98158.t1;Parent=MS.gene98158 |
| chr4.1 | exon | 88996909 | 88997084 | 88996909 | ID=MS.gene98158.t1.exon1;Parent=MS.gene98158.t1 |
| chr4.1 | CDS | 88996909 | 88997084 | 88996909 | ID=cds.MS.gene98158.t1;Parent=MS.gene98158.t1 |
| chr4.1 | exon | 88995318 | 88996585 | 88995318 | ID=MS.gene98158.t1.exon2;Parent=MS.gene98158.t1 |
| chr4.1 | CDS | 88995318 | 88996585 | 88995318 | ID=cds.MS.gene98158.t1;Parent=MS.gene98158.t1 |
| chr4.1 | exon | 88994217 | 88994508 | 88994217 | ID=MS.gene98158.t1.exon3;Parent=MS.gene98158.t1 |
| chr4.1 | CDS | 88994217 | 88994508 | 88994217 | ID=cds.MS.gene98158.t1;Parent=MS.gene98158.t1 |
| chr4.1 | exon | 88993821 | 88994112 | 88993821 | ID=MS.gene98158.t1.exon4;Parent=MS.gene98158.t1 |
| chr4.1 | CDS | 88993821 | 88994112 | 88993821 | ID=cds.MS.gene98158.t1;Parent=MS.gene98158.t1 |
| chr4.1 | exon | 88993395 | 88993740 | 88993395 | ID=MS.gene98158.t1.exon5;Parent=MS.gene98158.t1 |
| chr4.1 | CDS | 88993395 | 88993740 | 88993395 | ID=cds.MS.gene98158.t1;Parent=MS.gene98158.t1 |
| chr4.1 | exon | 88990044 | 88990244 | 88990044 | ID=MS.gene98158.t1.exon6;Parent=MS.gene98158.t1 |
| chr4.1 | CDS | 88990044 | 88990244 | 88990044 | ID=cds.MS.gene98158.t1;Parent=MS.gene98158.t1 |
| chr4.1 | exon | 88989466 | 88989704 | 88989466 | ID=MS.gene98158.t1.exon7;Parent=MS.gene98158.t1 |
| chr4.1 | CDS | 88989466 | 88989704 | 88989466 | ID=cds.MS.gene98158.t1;Parent=MS.gene98158.t1 |
| chr4.1 | exon | 88989273 | 88989353 | 88989273 | ID=MS.gene98158.t1.exon8;Parent=MS.gene98158.t1 |
| chr4.1 | CDS | 88989273 | 88989353 | 88989273 | ID=cds.MS.gene98158.t1;Parent=MS.gene98158.t1 |
| chr4.1 | exon | 88988049 | 88988153 | 88988049 | ID=MS.gene98158.t1.exon9;Parent=MS.gene98158.t1 |
| chr4.1 | CDS | 88988049 | 88988153 | 88988049 | ID=cds.MS.gene98158.t1;Parent=MS.gene98158.t1 |
| chr4.1 | exon | 88987756 | 88987905 | 88987756 | ID=MS.gene98158.t1.exon10;Parent=MS.gene98158.t1 |
| chr4.1 | CDS | 88987756 | 88987905 | 88987756 | ID=cds.MS.gene98158.t1;Parent=MS.gene98158.t1 |
| chr4.1 | exon | 88987277 | 88987441 | 88987277 | ID=MS.gene98158.t1.exon11;Parent=MS.gene98158.t1 |
| chr4.1 | CDS | 88987277 | 88987441 | 88987277 | ID=cds.MS.gene98158.t1;Parent=MS.gene98158.t1 |
| chr4.1 | exon | 88986418 | 88986504 | 88986418 | ID=MS.gene98158.t1.exon12;Parent=MS.gene98158.t1 |
| chr4.1 | CDS | 88986418 | 88986504 | 88986418 | ID=cds.MS.gene98158.t1;Parent=MS.gene98158.t1 |
| Gene Sequence |
| Protein sequence |
