Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98751.t1 | XP_003598516.1 | 99 | 97 | 1 | 0 | 3 | 99 | 233 | 329 | 4.50E-50 | 206.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98751.t1 | Q94CK9 | 53.2 | 79 | 37 | 0 | 7 | 85 | 220 | 298 | 6.6e-18 | 91.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98751.t1 | G7J0K6 | 99.0 | 97 | 1 | 0 | 3 | 99 | 233 | 329 | 3.2e-50 | 206.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene98751.t1 | TF | SRS |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049350 | MS.gene98751 | 0.810601 | 1.06E-50 | -1.69E-46 |
| MS.gene049807 | MS.gene98751 | 0.807929 | 3.98E-50 | -1.69E-46 |
| MS.gene053536 | MS.gene98751 | 0.808527 | 2.96E-50 | -1.69E-46 |
| MS.gene053538 | MS.gene98751 | 0.810478 | 1.13E-50 | -1.69E-46 |
| MS.gene054142 | MS.gene98751 | 0.806715 | 7.19E-50 | -1.69E-46 |
| MS.gene055537 | MS.gene98751 | 0.805003 | 1.65E-49 | -1.69E-46 |
| MS.gene05894 | MS.gene98751 | 0.832046 | 1.18E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98751.t1 | MTR_3g014660 | 98.969 | 97 | 1 | 0 | 3 | 99 | 233 | 329 | 2.99e-66 | 202 |
| MS.gene98751.t1 | MTR_8g039110 | 76.768 | 99 | 19 | 4 | 3 | 98 | 229 | 326 | 4.19e-42 | 140 |
| MS.gene98751.t1 | MTR_5g089750 | 62.319 | 69 | 26 | 0 | 3 | 71 | 348 | 416 | 1.58e-25 | 98.6 |
| MS.gene98751.t1 | MTR_5g021130 | 51.020 | 49 | 24 | 0 | 10 | 58 | 205 | 253 | 3.54e-12 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98751.t1 | AT5G12330 | 51.648 | 91 | 43 | 1 | 5 | 94 | 218 | 308 | 4.64e-23 | 90.5 |
| MS.gene98751.t1 | AT5G12330 | 51.648 | 91 | 43 | 1 | 5 | 94 | 218 | 308 | 4.64e-23 | 90.5 |
| MS.gene98751.t1 | AT5G12330 | 51.648 | 91 | 43 | 1 | 5 | 94 | 305 | 395 | 5.61e-23 | 91.3 |
| MS.gene98751.t1 | AT3G54430 | 58.209 | 67 | 27 | 1 | 3 | 68 | 116 | 182 | 8.14e-21 | 82.0 |
| MS.gene98751.t1 | AT1G19790 | 55.319 | 47 | 21 | 0 | 10 | 56 | 223 | 269 | 1.43e-12 | 62.0 |
| MS.gene98751.t1 | AT1G19790 | 55.319 | 47 | 21 | 0 | 10 | 56 | 223 | 269 | 1.43e-12 | 62.0 |
| MS.gene98751.t1 | AT5G66350 | 53.191 | 47 | 22 | 0 | 10 | 56 | 214 | 260 | 2.38e-11 | 58.5 |
| MS.gene98751.t1 | AT5G66350 | 53.191 | 47 | 22 | 0 | 10 | 56 | 214 | 260 | 2.65e-11 | 58.5 |
| MS.gene98751.t1 | AT3G51060 | 55.556 | 45 | 20 | 0 | 10 | 54 | 238 | 282 | 4.40e-11 | 58.2 |
Find 27 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCATGCACCAGCTGTATTC+AGG | 0.332427 | 3.4:+10897993 | MS.gene98751:CDS |
| GCAGGGTAAGCATTATTGTT+TGG | 0.337600 | 3.4:-10898217 | None:intergenic |
| AAAGGGTTTCTTTATGATCA+TGG | 0.374071 | 3.4:+10898097 | MS.gene98751:CDS |
| CTTTATGATCATGGTGTTGA+TGG | 0.390879 | 3.4:+10898106 | MS.gene98751:CDS |
| TGCCAATAGCAGTTACTCTA+TGG | 0.398017 | 3.4:-10898018 | None:intergenic |
| TTGTGTTTCAGAACTTCAAT+TGG | 0.409173 | 3.4:+10898144 | MS.gene98751:CDS |
| TTGGCCACAGTTCATATTAG+TGG | 0.413737 | 3.4:+10898064 | MS.gene98751:CDS |
| TGAAGATGAATTTGCTTACT+TGG | 0.421514 | 3.4:+10898045 | MS.gene98751:CDS |
| CATGGCTAGCTAGCAGAAGC+AGG | 0.449543 | 3.4:-10898235 | None:intergenic |
| TTCTGTTGAATGGCAGATGC+AGG | 0.451941 | 3.4:+10897944 | None:intergenic |
| TGCCATAGAGTAACTGCTAT+TGG | 0.452645 | 3.4:+10898016 | MS.gene98751:CDS |
| GGTGAATGTTCTTCTGCAAT+TGG | 0.456180 | 3.4:+10898190 | MS.gene98751:CDS |
| GGTTTCAAAGAGGGTTTACC+AGG | 0.457703 | 3.4:+10897965 | MS.gene98751:CDS |
| AGTTCTGAAACACAAGGCAT+TGG | 0.476027 | 3.4:-10898136 | None:intergenic |
| TGTGTTTCAGAACTTCAATT+GGG | 0.498389 | 3.4:+10898145 | MS.gene98751:CDS |
| AATAATTGTAGTGGAAAGAA+TGG | 0.512653 | 3.4:+10898169 | MS.gene98751:CDS |
| GCAGATGCAGGTTTCAAAGA+GGG | 0.558109 | 3.4:+10897956 | MS.gene98751:CDS |
| CTATGGCACCTGAATACAGC+TGG | 0.563710 | 3.4:-10898001 | None:intergenic |
| AGAGTAACTGCTATTGGCAA+TGG | 0.579028 | 3.4:+10898022 | MS.gene98751:CDS |
| CAATTGGGAAATAATTGTAG+TGG | 0.580003 | 3.4:+10898160 | MS.gene98751:CDS |
| AAAGAAACCCTTTGAAAACA+TGG | 0.590040 | 3.4:-10898087 | None:intergenic |
| TTTATGATCATGGTGTTGAT+GGG | 0.601430 | 3.4:+10898107 | MS.gene98751:CDS |
| GGCAGATGCAGGTTTCAAAG+AGG | 0.618391 | 3.4:+10897955 | MS.gene98751:CDS |
| ATGGCTAGCTAGCAGAAGCA+GGG | 0.656680 | 3.4:-10898234 | None:intergenic |
| GCTGGTGCATGAACATGACC+TGG | 0.691114 | 3.4:-10897983 | None:intergenic |
| ATGGCCACTAATATGAACTG+TGG | 0.704516 | 3.4:-10898068 | None:intergenic |
| AATTGAAGTTCTGAAACACA+AGG | 0.729820 | 3.4:-10898142 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAATTGTAGTGGAAAGAA+TGG | + | chr3.4:10898169-10898188 | MS.gene98751:CDS | 25.0% |
| AAAGGGTTTCTTTATGATCA+TGG | + | chr3.4:10898097-10898116 | MS.gene98751:CDS | 30.0% | |
| CAATTGGGAAATAATTGTAG+TGG | + | chr3.4:10898160-10898179 | MS.gene98751:CDS | 30.0% | |
| TGTGTTTCAGAACTTCAATT+GGG | + | chr3.4:10898145-10898164 | MS.gene98751:CDS | 30.0% | |
| TTGTGTTTCAGAACTTCAAT+TGG | + | chr3.4:10898144-10898163 | MS.gene98751:CDS | 30.0% | |
| ! | AAAGAAACCCTTTGAAAACA+TGG | - | chr3.4:10898090-10898109 | None:intergenic | 30.0% |
| ! | TGAAGATGAATTTGCTTACT+TGG | + | chr3.4:10898045-10898064 | MS.gene98751:CDS | 30.0% |
| !! | AATTGAAGTTCTGAAACACA+AGG | - | chr3.4:10898145-10898164 | None:intergenic | 30.0% |
| !! | TTTATGATCATGGTGTTGAT+GGG | + | chr3.4:10898107-10898126 | MS.gene98751:CDS | 30.0% |
| ! | ATTAGTGGCCATGTTTTCAA+AGG | + | chr3.4:10898079-10898098 | MS.gene98751:CDS | 35.0% |
| ! | CTTTATGATCATGGTGTTGA+TGG | + | chr3.4:10898106-10898125 | MS.gene98751:CDS | 35.0% |
| ! | TTAGTGGCCATGTTTTCAAA+GGG | + | chr3.4:10898080-10898099 | MS.gene98751:CDS | 35.0% |
| AGAGTAACTGCTATTGGCAA+TGG | + | chr3.4:10898022-10898041 | MS.gene98751:CDS | 40.0% | |
| ATGGCCACTAATATGAACTG+TGG | - | chr3.4:10898071-10898090 | None:intergenic | 40.0% | |
| GCAGGGTAAGCATTATTGTT+TGG | - | chr3.4:10898220-10898239 | None:intergenic | 40.0% | |
| GGTGAATGTTCTTCTGCAAT+TGG | + | chr3.4:10898190-10898209 | MS.gene98751:CDS | 40.0% | |
| TGCCAATAGCAGTTACTCTA+TGG | - | chr3.4:10898021-10898040 | None:intergenic | 40.0% | |
| TGCCATAGAGTAACTGCTAT+TGG | + | chr3.4:10898016-10898035 | MS.gene98751:CDS | 40.0% | |
| TTGGCCACAGTTCATATTAG+TGG | + | chr3.4:10898064-10898083 | MS.gene98751:CDS | 40.0% | |
| !! | AGTTCTGAAACACAAGGCAT+TGG | - | chr3.4:10898139-10898158 | None:intergenic | 40.0% |
| GCAGATGCAGGTTTCAAAGA+GGG | + | chr3.4:10897956-10897975 | MS.gene98751:CDS | 45.0% | |
| GGTTTCAAAGAGGGTTTACC+AGG | + | chr3.4:10897965-10897984 | MS.gene98751:CDS | 45.0% | |
| TTCATGCACCAGCTGTATTC+AGG | + | chr3.4:10897993-10898012 | MS.gene98751:CDS | 45.0% | |
| CTATGGCACCTGAATACAGC+TGG | - | chr3.4:10898004-10898023 | None:intergenic | 50.0% | |
| GGCAGATGCAGGTTTCAAAG+AGG | + | chr3.4:10897955-10897974 | MS.gene98751:CDS | 50.0% | |
| GCTGGTGCATGAACATGACC+TGG | - | chr3.4:10897986-10898005 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 10897953 | 10898252 | 10897953 | ID=MS.gene98751 |
| chr3.4 | mRNA | 10897953 | 10898252 | 10897953 | ID=MS.gene98751.t1;Parent=MS.gene98751 |
| chr3.4 | exon | 10897953 | 10898252 | 10897953 | ID=MS.gene98751.t1.exon1;Parent=MS.gene98751.t1 |
| chr3.4 | CDS | 10897953 | 10898252 | 10897953 | ID=cds.MS.gene98751.t1;Parent=MS.gene98751.t1 |
| Gene Sequence |
| Protein sequence |
