Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98791.t1 | RHN69131.1 | 82.4 | 159 | 27 | 1 | 1 | 159 | 614 | 771 | 4.80E-65 | 257.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98791.t1 | A0A396IWT8 | 82.4 | 159 | 27 | 1 | 1 | 159 | 614 | 771 | 3.4e-65 | 257.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05344 | MS.gene98791 | 0.808305 | 3.31E-50 | -1.69E-46 |
| MS.gene057452 | MS.gene98791 | 0.803809 | 2.92E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98791.t1 | MTR_3g083770 | 80.745 | 161 | 30 | 1 | 1 | 161 | 373 | 532 | 1.03e-72 | 228 |
| MS.gene98791.t1 | MTR_3g083770 | 80.745 | 161 | 30 | 1 | 1 | 161 | 613 | 772 | 1.49e-71 | 229 |
| MS.gene98791.t1 | MTR_3g083770 | 80.745 | 161 | 30 | 1 | 1 | 161 | 614 | 773 | 1.49e-71 | 230 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 60 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACGTCTCTGCTGCTCCTTT+TGG | 0.246885 | 3.4:-78633365 | MS.gene98791:CDS |
| GGCCTCCATGCCTTCAAATT+CGG | 0.255606 | 3.4:-78633594 | None:intergenic |
| GTCCGCGCACGCCGCCATTT+AGG | 0.269653 | 3.4:-78633463 | MS.gene98791:CDS |
| GCCTCCATGCCTTCAAATTC+GGG | 0.291164 | 3.4:-78633593 | None:intergenic |
| GTCGATTGTGCATACCTCTT+TGG | 0.316372 | 3.4:+78633192 | None:intergenic |
| TGAGTAGTAGCTTGACTGTT+TGG | 0.351937 | 3.4:+78633512 | None:intergenic |
| TTTGTGGGGACTTGAGATCT+TGG | 0.394613 | 3.4:+78633273 | None:intergenic |
| AAGCTTTGAAGAGGCTGATC+AGG | 0.402278 | 3.4:+78633431 | None:intergenic |
| TCAGGCACAGCAAACCTAAA+TGG | 0.414543 | 3.4:+78633449 | None:intergenic |
| GGTACACAAGAGCCACCTTT+TGG | 0.417527 | 3.4:-78633485 | MS.gene98791:CDS |
| AGATCTCAAGTCCCCACAAA+TGG | 0.421909 | 3.4:-78633270 | MS.gene98791:CDS |
| ATATGGAAATGCAGGGTGTC+TGG | 0.421915 | 3.4:+78633311 | None:intergenic |
| TGCATACCTCTTTGGACATC+AGG | 0.425484 | 3.4:+78633200 | None:intergenic |
| AGTAGCTTGACTGTTTGGTC+TGG | 0.429015 | 3.4:+78633517 | None:intergenic |
| TGCCCCTCAAATGCAAAACT+TGG | 0.437679 | 3.4:-78633228 | MS.gene98791:CDS |
| GTAAAATTTCCCGAATTTGA+AGG | 0.438768 | 3.4:+78633584 | None:intergenic |
| TCGACAAAGCTATCCTGCTC+AGG | 0.450978 | 3.4:-78633174 | MS.gene98791:CDS |
| GGCGGCGTGCGCGGACCAAA+AGG | 0.474482 | 3.4:+78633470 | None:intergenic |
| ATTTCCCGAATTTGAAGGCA+TGG | 0.481988 | 3.4:+78633589 | None:intergenic |
| TGACCAGCATATGGAAATGC+AGG | 0.484597 | 3.4:+78633303 | None:intergenic |
| GTAGCTTGACTGTTTGGTCT+GGG | 0.488055 | 3.4:+78633518 | None:intergenic |
| ACAATCCCTCCTGTGGCGTC+CGG | 0.492108 | 3.4:-78633121 | None:intergenic |
| AGAGGCCATGCTCCCTGAGC+AGG | 0.492143 | 3.4:+78633161 | None:intergenic |
| AAACAGTCAAGCTACTACTC+AGG | 0.493193 | 3.4:-78633510 | MS.gene98791:CDS |
| AAGTTCCTCATACCCATTTG+TGG | 0.495758 | 3.4:+78633257 | None:intergenic |
| CACCCTGCATTTCCATATGC+TGG | 0.496063 | 3.4:-78633306 | MS.gene98791:CDS |
| GGTGCAGGTTGACCAGCATA+TGG | 0.497213 | 3.4:+78633294 | None:intergenic |
| ACTTGATAGTAGTCTGCATT+GGG | 0.511196 | 3.4:+78633545 | None:intergenic |
| CACTTGATAGTAGTCTGCAT+TGG | 0.513755 | 3.4:+78633544 | None:intergenic |
| AGTCCCCACAAATGGGTATG+AGG | 0.516615 | 3.4:-78633262 | MS.gene98791:CDS |
| GGGACTTGAGATCTTGGTGC+AGG | 0.523652 | 3.4:+78633279 | None:intergenic |
| AGTTCCTCATACCCATTTGT+GGG | 0.526599 | 3.4:+78633258 | None:intergenic |
| TATGGAAATGCAGGGTGTCT+GGG | 0.533513 | 3.4:+78633312 | None:intergenic |
| AGCCTCTTCAAAGCTTTCAA+AGG | 0.535341 | 3.4:-78633424 | MS.gene98791:CDS |
| GATCTCAAGTCCCCACAAAT+GGG | 0.535833 | 3.4:-78633269 | MS.gene98791:CDS |
| GGTGGAAATGAGACAGAACC+TGG | 0.538205 | 3.4:+78633333 | None:intergenic |
| GCTATCCTGCTCAGGGAGCA+TGG | 0.543642 | 3.4:-78633166 | MS.gene98791:CDS |
| GGCTTACCGGACGCCACAGG+AGG | 0.546500 | 3.4:+78633115 | None:intergenic |
| GACACAAGTCTCCTACCAGC+TGG | 0.546697 | 3.4:-78633402 | MS.gene98791:CDS |
| TTGCTAAACTTCTGATTCAG+AGG | 0.548374 | 3.4:+78633143 | None:intergenic |
| GTCCTTTGAAAGCTTTGAAG+AGG | 0.552634 | 3.4:+78633422 | None:intergenic |
| AACCTAAATGGCGGCGTGCG+CGG | 0.558256 | 3.4:+78633461 | None:intergenic |
| GCTGGCTTACCGGACGCCAC+AGG | 0.564986 | 3.4:+78633112 | None:intergenic |
| GCAGAGACGTAAGCTTCAGA+TGG | 0.570381 | 3.4:+78633377 | None:intergenic |
| ACCTCTTTGGACATCAGGAC+TGG | 0.571413 | 3.4:+78633205 | None:intergenic |
| ATTGGGCCGCTGTCCTTGAA+AGG | 0.581635 | 3.4:+78633562 | None:intergenic |
| AACAGTCAAGCTACTACTCA+GGG | 0.591753 | 3.4:-78633509 | MS.gene98791:CDS |
| CGACAAAGCTATCCTGCTCA+GGG | 0.593335 | 3.4:-78633173 | MS.gene98791:CDS |
| GGCGTGCGCGGACCAAAAGG+TGG | 0.607996 | 3.4:+78633473 | None:intergenic |
| AAGCTTCAGATGGATCCAGC+TGG | 0.624556 | 3.4:+78633387 | None:intergenic |
| GCCAGTCCTGATGTCCAAAG+AGG | 0.645143 | 3.4:-78633206 | MS.gene98791:CDS |
| GTTCCTCATACCCATTTGTG+GGG | 0.648242 | 3.4:+78633259 | None:intergenic |
| GGAAATGCAGGGTGTCTGGG+TGG | 0.652818 | 3.4:+78633315 | None:intergenic |
| TTTAGCAACAATCCCTCCTG+TGG | 0.660630 | 3.4:-78633128 | MS.gene98791:CDS |
| GACCAGCATATGGAAATGCA+GGG | 0.662409 | 3.4:+78633304 | None:intergenic |
| CAATGCAGACTACTATCAAG+TGG | 0.664785 | 3.4:-78633543 | MS.gene98791:CDS |
| GGCACAGCAAACCTAAATGG+CGG | 0.665012 | 3.4:+78633452 | None:intergenic |
| GCTTACCGGACGCCACAGGA+GGG | 0.671577 | 3.4:+78633116 | None:intergenic |
| TTCAGATGGATCCAGCTGGT+AGG | 0.683202 | 3.4:+78633391 | None:intergenic |
| AGTCAAGCTACTACTCAGGG+TGG | 0.734032 | 3.4:-78633506 | MS.gene98791:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GTAAAATTTCCCGAATTTGA+AGG | + | chr3.4:78633128-78633147 | None:intergenic | 30.0% | |
| TTGCTAAACTTCTGATTCAG+AGG | + | chr3.4:78633569-78633588 | None:intergenic | 35.0% | |
| ! | ACTTGATAGTAGTCTGCATT+GGG | + | chr3.4:78633167-78633186 | None:intergenic | 35.0% |
| ! | ATTTTACCTTTCAAGGACAG+CGG | - | chr3.4:78633141-78633160 | MS.gene98791:CDS | 35.0% |
| ! | TCGGGAAATTTTACCTTTCA+AGG | - | chr3.4:78633134-78633153 | MS.gene98791:CDS | 35.0% |
| AAACAGTCAAGCTACTACTC+AGG | - | chr3.4:78633199-78633218 | MS.gene98791:CDS | 40.0% | |
| AACAGTCAAGCTACTACTCA+GGG | - | chr3.4:78633200-78633219 | MS.gene98791:CDS | 40.0% | |
| AAGTTCCTCATACCCATTTG+TGG | + | chr3.4:78633455-78633474 | None:intergenic | 40.0% | |
| AGCCTCTTCAAAGCTTTCAA+AGG | - | chr3.4:78633285-78633304 | MS.gene98791:CDS | 40.0% | |
| AGTTCCTCATACCCATTTGT+GGG | + | chr3.4:78633454-78633473 | None:intergenic | 40.0% | |
| CAATGCAGACTACTATCAAG+TGG | - | chr3.4:78633166-78633185 | MS.gene98791:CDS | 40.0% | |
| TGAGTAGTAGCTTGACTGTT+TGG | + | chr3.4:78633200-78633219 | None:intergenic | 40.0% | |
| ! | CACTTGATAGTAGTCTGCAT+TGG | + | chr3.4:78633168-78633187 | None:intergenic | 40.0% |
| ! | TGGCCAAGTTTTGCATTTGA+GGG | + | chr3.4:78633487-78633506 | None:intergenic | 40.0% |
| ! | TTTCCCGAATTTGAAGGCAT+CGG | + | chr3.4:78633122-78633141 | None:intergenic | 40.0% |
| !! | GTCCTTTGAAAGCTTTGAAG+AGG | + | chr3.4:78633290-78633309 | None:intergenic | 40.0% |
| AGATCTCAAGTCCCCACAAA+TGG | - | chr3.4:78633439-78633458 | MS.gene98791:CDS | 45.0% | |
| AGTAGCTTGACTGTTTGGTC+TGG | + | chr3.4:78633195-78633214 | None:intergenic | 45.0% | |
| GACCAGCATATGGAAATGCA+GGG | + | chr3.4:78633408-78633427 | None:intergenic | 45.0% | |
| GATCTCAAGTCCCCACAAAT+GGG | - | chr3.4:78633440-78633459 | MS.gene98791:CDS | 45.0% | |
| GTAGCTTGACTGTTTGGTCT+GGG | + | chr3.4:78633194-78633213 | None:intergenic | 45.0% | |
| GTCGATTGTGCATACCTCTT+TGG | + | chr3.4:78633520-78633539 | None:intergenic | 45.0% | |
| GTTCCTCATACCCATTTGTG+GGG | + | chr3.4:78633453-78633472 | None:intergenic | 45.0% | |
| TCAGGCACAGCAAACCTAAA+TGG | + | chr3.4:78633263-78633282 | None:intergenic | 45.0% | |
| TGACCAGCATATGGAAATGC+AGG | + | chr3.4:78633409-78633428 | None:intergenic | 45.0% | |
| TGCATACCTCTTTGGACATC+AGG | + | chr3.4:78633512-78633531 | None:intergenic | 45.0% | |
| TGCCCCTCAAATGCAAAACT+TGG | - | chr3.4:78633481-78633500 | MS.gene98791:CDS | 45.0% | |
| TTTAGCAACAATCCCTCCTG+TGG | - | chr3.4:78633581-78633600 | MS.gene98791:CDS | 45.0% | |
| TTTGTGGGGACTTGAGATCT+TGG | + | chr3.4:78633439-78633458 | None:intergenic | 45.0% | |
| ! | AAGTTTTGCATTTGAGGGGC+AGG | + | chr3.4:78633482-78633501 | None:intergenic | 45.0% |
| ! | ATATGGAAATGCAGGGTGTC+TGG | + | chr3.4:78633401-78633420 | None:intergenic | 45.0% |
| ! | CTGGCCAAGTTTTGCATTTG+AGG | + | chr3.4:78633488-78633507 | None:intergenic | 45.0% |
| ! | GGCCAAGTTTTGCATTTGAG+GGG | + | chr3.4:78633486-78633505 | None:intergenic | 45.0% |
| ! | TATGGAAATGCAGGGTGTCT+GGG | + | chr3.4:78633400-78633419 | None:intergenic | 45.0% |
| !! | AAGCTTTGAAGAGGCTGATC+AGG | + | chr3.4:78633281-78633300 | None:intergenic | 45.0% |
| AAGCTTCAGATGGATCCAGC+TGG | + | chr3.4:78633325-78633344 | None:intergenic | 50.0% | |
| ACCTCTTTGGACATCAGGAC+TGG | + | chr3.4:78633507-78633526 | None:intergenic | 50.0% | |
| AGTCAAGCTACTACTCAGGG+TGG | - | chr3.4:78633203-78633222 | MS.gene98791:CDS | 50.0% | |
| AGTCCCCACAAATGGGTATG+AGG | - | chr3.4:78633447-78633466 | MS.gene98791:CDS | 50.0% | |
| CACCCTGCATTTCCATATGC+TGG | - | chr3.4:78633403-78633422 | MS.gene98791:CDS | 50.0% | |
| CGACAAAGCTATCCTGCTCA+GGG | - | chr3.4:78633536-78633555 | MS.gene98791:CDS | 50.0% | |
| GCAGAGACGTAAGCTTCAGA+TGG | + | chr3.4:78633335-78633354 | None:intergenic | 50.0% | |
| GGCACAGCAAACCTAAATGG+CGG | + | chr3.4:78633260-78633279 | None:intergenic | 50.0% | |
| GGTGGAAATGAGACAGAACC+TGG | + | chr3.4:78633379-78633398 | None:intergenic | 50.0% | |
| TCGACAAAGCTATCCTGCTC+AGG | - | chr3.4:78633535-78633554 | MS.gene98791:CDS | 50.0% | |
| TTCAGATGGATCCAGCTGGT+AGG | + | chr3.4:78633321-78633340 | None:intergenic | 50.0% | |
| ! | ACGTCTCTGCTGCTCCTTTT+GGG | - | chr3.4:78633345-78633364 | MS.gene98791:CDS | 50.0% |
| ! | GGTACACAAGAGCCACCTTT+TGG | - | chr3.4:78633224-78633243 | MS.gene98791:CDS | 50.0% |
| ! | TACGTCTCTGCTGCTCCTTT+TGG | - | chr3.4:78633344-78633363 | MS.gene98791:CDS | 50.0% |
| ACCTGGAACTTCCCCCCAAA+AGG | + | chr3.4:78633362-78633381 | None:intergenic | 55.0% | |
| ATTGGGCCGCTGTCCTTGAA+AGG | + | chr3.4:78633150-78633169 | None:intergenic | 55.0% | |
| GACACAAGTCTCCTACCAGC+TGG | - | chr3.4:78633307-78633326 | MS.gene98791:CDS | 55.0% | |
| GCCAGTCCTGATGTCCAAAG+AGG | - | chr3.4:78633503-78633522 | MS.gene98791:CDS | 55.0% | |
| ! | CGTCTCTGCTGCTCCTTTTG+GGG | - | chr3.4:78633346-78633365 | MS.gene98791:CDS | 55.0% |
| ! | GGGACTTGAGATCTTGGTGC+AGG | + | chr3.4:78633433-78633452 | None:intergenic | 55.0% |
| ! | GGTGCAGGTTGACCAGCATA+TGG | + | chr3.4:78633418-78633437 | None:intergenic | 55.0% |
| ! | GTCTCTGCTGCTCCTTTTGG+GGG | - | chr3.4:78633347-78633366 | MS.gene98791:CDS | 55.0% |
| ! | TCTCTGCTGCTCCTTTTGGG+GGG | - | chr3.4:78633348-78633367 | MS.gene98791:CDS | 55.0% |
| !! | TCCTTTTGGGGGGAAGTTCC+AGG | - | chr3.4:78633358-78633377 | MS.gene98791:CDS | 55.0% |
| AACCTAAATGGCGGCGTGCG+CGG | + | chr3.4:78633251-78633270 | None:intergenic | 60.0% | |
| GCTATCCTGCTCAGGGAGCA+TGG | - | chr3.4:78633543-78633562 | MS.gene98791:CDS | 60.0% | |
| ! | GGAAATGCAGGGTGTCTGGG+TGG | + | chr3.4:78633397-78633416 | None:intergenic | 60.0% |
| AGAGGCCATGCTCCCTGAGC+AGG | + | chr3.4:78633551-78633570 | None:intergenic | 65.0% | |
| GGCGTGCGCGGACCAAAAGG+TGG | + | chr3.4:78633239-78633258 | None:intergenic | 70.0% | |
| GTCCGCGCACGCCGCCATTT+AGG | - | chr3.4:78633246-78633265 | MS.gene98791:CDS | 70.0% | |
| GGCGGCGTGCGCGGACCAAA+AGG | + | chr3.4:78633242-78633261 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 78633122 | 78633609 | 78633122 | ID=MS.gene98791 |
| chr3.4 | mRNA | 78633122 | 78633609 | 78633122 | ID=MS.gene98791.t1;Parent=MS.gene98791 |
| chr3.4 | exon | 78633122 | 78633609 | 78633122 | ID=MS.gene98791.t1.exon1;Parent=MS.gene98791.t1 |
| chr3.4 | CDS | 78633122 | 78633609 | 78633122 | ID=cds.MS.gene98791.t1;Parent=MS.gene98791.t1 |
| Gene Sequence |
| Protein sequence |
