Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99009.t1 | KEH16865.1 | 99.3 | 285 | 2 | 0 | 1 | 285 | 14 | 298 | 6.90E-160 | 573.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99009.t1 | P93286 | 84.5 | 251 | 31 | 4 | 1 | 247 | 14 | 260 | 1.4e-108 | 394.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99009.t1 | A0A072TTM0 | 99.3 | 285 | 2 | 0 | 1 | 285 | 14 | 298 | 5.0e-160 | 573.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050807 | MS.gene99009 | -0.822294 | 2.56E-53 | -1.69E-46 |
| MS.gene050808 | MS.gene99009 | -0.800214 | 1.60E-48 | -1.69E-46 |
| MS.gene052239 | MS.gene99009 | 0.817916 | 2.57E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99009.t1 | MTR_0082s0260 | 99.298 | 285 | 2 | 0 | 1 | 285 | 14 | 298 | 0.0 | 579 |
| MS.gene99009.t1 | MTR_0024s0140 | 98.268 | 231 | 4 | 0 | 55 | 285 | 1 | 231 | 2.64e-167 | 462 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99009.t1 | ATMG00180 | 86.056 | 251 | 27 | 4 | 1 | 247 | 14 | 260 | 1.17e-124 | 363 |
| MS.gene99009.t1 | AT2G07815 | 92.063 | 63 | 5 | 0 | 1 | 63 | 186 | 248 | 1.94e-31 | 118 |
Find 0 sgRNAs with CRISPR-Local
Find 126 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGATGAATATTTCACATTT+TGG | - | 6917:24771-24790 | MS.gene99009:CDS | 20.0% |
| ! | ATTATGTCTGGTAATAGAAT+AGG | + | 6917:24294-24313 | None:intergenic | 25.0% |
| ! | ATTCTATTACCAGACATAAT+TGG | - | 6917:24294-24313 | MS.gene99009:CDS | 25.0% |
| ! | TGTGAAATATTCATCTTCAT+TGG | + | 6917:24768-24787 | None:intergenic | 25.0% |
| ! | TTATGTCTGGTAATAGAATA+GGG | + | 6917:24293-24312 | None:intergenic | 25.0% |
| ! | TTCTATTACCAGACATAATT+GGG | - | 6917:24295-24314 | MS.gene99009:CDS | 25.0% |
| !! | TCATATTTAGAATCTTTCTG+CGG | - | 6917:24408-24427 | MS.gene99009:CDS | 25.0% |
| AATAGGAATTATAGTCCCAT+TGG | + | 6917:24172-24191 | None:intergenic | 30.0% | |
| AATGTAGATAGACCAAAAGA+GGG | + | 6917:24209-24228 | None:intergenic | 30.0% | |
| ACGAACCATTTCAATAGTAA+GGG | + | 6917:24117-24136 | None:intergenic | 30.0% | |
| ATAAGAAGGAAATGAAGAAC+GGG | + | 6917:24369-24388 | None:intergenic | 30.0% | |
| ATTATAGTCCCATTGGAAAA+AGG | + | 6917:24165-24184 | None:intergenic | 30.0% | |
| GATAAGAAGGAAATGAAGAA+CGG | + | 6917:24370-24389 | None:intergenic | 30.0% | |
| TTATAGTCCCATTGGAAAAA+GGG | + | 6917:24164-24183 | None:intergenic | 30.0% | |
| AACAACGAAATGACAAGATG+GGG | - | 6917:24553-24572 | MS.gene99009:CDS | 35.0% | |
| CAACAACGAAATGACAAGAT+GGG | - | 6917:24552-24571 | MS.gene99009:CDS | 35.0% | |
| GAATGTAGATAGACCAAAAG+AGG | + | 6917:24210-24229 | None:intergenic | 35.0% | |
| GACGAACCATTTCAATAGTA+AGG | + | 6917:24118-24137 | None:intergenic | 35.0% | |
| TATAGTCCCATTGGAAAAAG+GGG | + | 6917:24163-24182 | None:intergenic | 35.0% | |
| TTCCTTCTTATCGAATCCAA+GGG | - | 6917:24378-24397 | MS.gene99009:CDS | 35.0% | |
| TTCGACCCTTCTTTCTAAAT+GGG | + | 6917:24592-24611 | None:intergenic | 35.0% | |
| TTTCCTTCTTATCGAATCCA+AGG | - | 6917:24377-24396 | MS.gene99009:CDS | 35.0% | |
| ! | TCATCTTCATTGGAGCTTTT+TGG | + | 6917:24758-24777 | None:intergenic | 35.0% |
| ! | TTTTGGTCTATCTACATTCC+AGG | - | 6917:24211-24230 | MS.gene99009:CDS | 35.0% |
| AAATTCATACGCTCCATGGA+CGG | - | 6917:24234-24253 | MS.gene99009:CDS | 40.0% | |
| AGACCAAAAGAGGGAATGAA+GGG | + | 6917:24200-24219 | None:intergenic | 40.0% | |
| CAGCACCCTTACTATTGAAA+TGG | - | 6917:24109-24128 | MS.gene99009:CDS | 40.0% | |
| CATTTAGAAAGAAGGGTCGA+AGG | - | 6917:24591-24610 | MS.gene99009:CDS | 40.0% | |
| CTTCGACCCTTCTTTCTAAA+TGG | + | 6917:24593-24612 | None:intergenic | 40.0% | |
| GAAGGATACACCAAAGACTT+AGG | - | 6917:24508-24527 | MS.gene99009:CDS | 40.0% | |
| GCAACAACGAAATGACAAGA+TGG | - | 6917:24551-24570 | MS.gene99009:CDS | 40.0% | |
| GGGAATGAAGGGATAAGAAT+AGG | + | 6917:24189-24208 | None:intergenic | 40.0% | |
| TAAATATGAGAAGTCCCCCT+TGG | + | 6917:24397-24416 | None:intergenic | 40.0% | |
| TAGACCAAAAGAGGGAATGA+AGG | + | 6917:24201-24220 | None:intergenic | 40.0% | |
| TCCTTCTTATCGAATCCAAG+GGG | - | 6917:24379-24398 | MS.gene99009:CDS | 40.0% | |
| TCGACCCTTCTTTCTAAATG+GGG | + | 6917:24591-24610 | None:intergenic | 40.0% | |
| TGTCTGGTAATAGAATAGGG+CGG | + | 6917:24290-24309 | None:intergenic | 40.0% | |
| ! | AAAGAAGGGTCGAAGGTTTT+GGG | - | 6917:24598-24617 | MS.gene99009:CDS | 40.0% |
| ! | TATCCCTTCATTCCCTCTTT+TGG | - | 6917:24194-24213 | MS.gene99009:CDS | 40.0% |
| ! | TCTTCATTGGAGCTTTTTGG+TGG | + | 6917:24755-24774 | None:intergenic | 40.0% |
| AACGGGAACGAAACGAAATG+AGG | + | 6917:24352-24371 | None:intergenic | 45.0% | |
| AGACTTAGGCCTAACCCTAA+CGG | - | 6917:24522-24541 | MS.gene99009:CDS | 45.0% | |
| CAGGAAATTCATACGCTCCA+TGG | - | 6917:24230-24249 | MS.gene99009:CDS | 45.0% | |
| CATGGAGCGTATGAATTTCC+TGG | + | 6917:24232-24251 | None:intergenic | 45.0% | |
| CCTTCTTATCGAATCCAAGG+GGG | - | 6917:24380-24399 | MS.gene99009:CDS | 45.0% | |
| CGACCCTTCTTTCTAAATGG+GGG | + | 6917:24590-24609 | None:intergenic | 45.0% | |
| GAGCTTCTCCCAATTATGTC+TGG | + | 6917:24306-24325 | None:intergenic | 45.0% | |
| GCACCCCCATTTAGAAAGAA+GGG | - | 6917:24584-24603 | MS.gene99009:CDS | 45.0% | |
| GGAGTGTGCATCTGTATGTT+GGG | - | 6917:24792-24811 | MS.gene99009:CDS | 45.0% | |
| GTTGTTGCTCATTCCCGTTA+GGG | + | 6917:24539-24558 | None:intergenic | 45.0% | |
| TAGGGTTAGGCCTAAGTCTT+TGG | + | 6917:24521-24540 | None:intergenic | 45.0% | |
| TCTTCTCTTTACCACGCGAT+AGG | - | 6917:24460-24479 | MS.gene99009:CDS | 45.0% | |
| TGGAGTGTGCATCTGTATGT+TGG | - | 6917:24791-24810 | MS.gene99009:CDS | 45.0% | |
| ! | GAAAGAAGGGTCGAAGGTTT+TGG | - | 6917:24597-24616 | MS.gene99009:CDS | 45.0% |
| AGAGCTTCAAGCCCGATTTC+AGG | + | 6917:24687-24706 | None:intergenic | 50.0% | |
| AGCTCATCCGAAACTAGAGC+TGG | - | 6917:24321-24340 | MS.gene99009:CDS | 50.0% | |
| ATAGGTCAGCAAAGCGTGAG+CGG | - | 6917:24478-24497 | MS.gene99009:CDS | 50.0% | |
| CCCCCTTGGATTCGATAAGA+AGG | + | 6917:24383-24402 | None:intergenic | 50.0% | |
| CGGAGCAAAAAGTGGAGTCT+TGG | - | 6917:24254-24273 | MS.gene99009:CDS | 50.0% | |
| CGTTGTTGCTCATTCCCGTT+AGG | + | 6917:24540-24559 | None:intergenic | 50.0% | |
| GACTTAGGCCTAACCCTAAC+GGG | - | 6917:24523-24542 | MS.gene99009:CDS | 50.0% | |
| GGCACCCCCATTTAGAAAGA+AGG | - | 6917:24583-24602 | MS.gene99009:CDS | 50.0% | |
| GTCAGTAGAGATGTTCCCAC+AGG | - | 6917:24135-24154 | MS.gene99009:CDS | 50.0% | |
| GTGCCACCTGAACCTGAAAT+CGG | - | 6917:24672-24691 | MS.gene99009:CDS | 50.0% | |
| TCCATGGACGGAGCAAAAAG+TGG | - | 6917:24246-24265 | MS.gene99009:CDS | 50.0% | |
| TGCCACCTGAACCTGAAATC+GGG | - | 6917:24673-24692 | MS.gene99009:CDS | 50.0% | |
| TGCTCATTCCCGTTAGGGTT+AGG | + | 6917:24534-24553 | None:intergenic | 50.0% | |
| ! | AATGACAAGATGGGGTGCTC+CGG | - | 6917:24561-24580 | MS.gene99009:CDS | 50.0% |
| ! | ACAGGTGCCCCTTTTTCCAA+TGG | - | 6917:24153-24172 | MS.gene99009:CDS | 50.0% |
| ! | CAGGTGCCCCTTTTTCCAAT+GGG | - | 6917:24154-24173 | MS.gene99009:CDS | 50.0% |
| ! | GGCGTTTCCAGCTCTAGTTT+CGG | + | 6917:24331-24350 | None:intergenic | 50.0% |
| !! | ATTGGAAAAAGGGGCACCTG+TGG | + | 6917:24154-24173 | None:intergenic | 50.0% |
| !! | TCAAGCCCGATTTCAGGTTC+AGG | + | 6917:24681-24700 | None:intergenic | 50.0% |
| !! | TCTTTCTAAATGGGGGTGCC+CGG | + | 6917:24583-24602 | None:intergenic | 50.0% |
| !! | TTGGAAAAAGGGGCACCTGT+GGG | + | 6917:24153-24172 | None:intergenic | 50.0% |
| !!! | TCCACTTTTTGCTCCGTCCA+TGG | + | 6917:24250-24269 | None:intergenic | 50.0% |
| CCACGCCTCCTATTTGTTCG+GGG | - | 6917:24860-24879 | MS.gene99009:CDS | 55.0% | |
| CCCACGCCTCCTATTTGTTC+GGG | - | 6917:24859-24878 | MS.gene99009:CDS | 55.0% | |
| GACCAACAGCCATCAGCTGT+CGG | + | 6917:24725-24744 | None:intergenic | 55.0% | |
| GCCCACGCCTCCTATTTGTT+CGG | - | 6917:24858-24877 | MS.gene99009:CDS | 55.0% | |
| TAGGTCAGCAAAGCGTGAGC+GGG | - | 6917:24479-24498 | MS.gene99009:CDS | 55.0% | |
| TCAACTGAGCATCTCAGCGG+CGG | + | 6917:24925-24944 | None:intergenic | 55.0% | |
| TGTTCTTCTGTCGTGCGACC+CGG | - | 6917:24815-24834 | MS.gene99009:CDS | 55.0% | |
| ! | ATGACAAGATGGGGTGCTCC+GGG | - | 6917:24562-24581 | MS.gene99009:CDS | 55.0% |
| ! | CCCGAACAAATAGGAGGCGT+GGG | + | 6917:24862-24881 | None:intergenic | 55.0% |
| ! | CGTGAACTCTGACTCGATCC+GGG | - | 6917:24890-24909 | MS.gene99009:CDS | 55.0% |
| ! | GCGTGAACTCTGACTCGATC+CGG | - | 6917:24889-24908 | MS.gene99009:CDS | 55.0% |
| !! | AGCCCGATTTCAGGTTCAGG+TGG | + | 6917:24678-24697 | None:intergenic | 55.0% |
| ACAAGCACCACCCGACGAAG+GGG | + | 6917:24643-24662 | None:intergenic | 60.0% | |
| ACACAAGCACCACCCGACGA+AGG | + | 6917:24645-24664 | None:intergenic | 60.0% | |
| ACAGGTCGCACATAAGCCGC+CGG | + | 6917:24837-24856 | None:intergenic | 60.0% | |
| ACCAACGAGCCGACAGCTGA+TGG | - | 6917:24713-24732 | MS.gene99009:CDS | 60.0% | |
| AGCCGACAGCTGATGGCTGT+TGG | - | 6917:24720-24739 | MS.gene99009:CDS | 60.0% | |
| CAACTGAGCATCTCAGCGGC+GGG | + | 6917:24924-24943 | None:intergenic | 60.0% | |
| CACAAGCACCACCCGACGAA+GGG | + | 6917:24644-24663 | None:intergenic | 60.0% | |
| CAGCGGCGGGATTGAATACC+CGG | + | 6917:24911-24930 | None:intergenic | 60.0% | |
| CCCCGAACAAATAGGAGGCG+TGG | + | 6917:24863-24882 | None:intergenic | 60.0% | |
| CGCCTCCTATTTGTTCGGGG+CGG | - | 6917:24863-24882 | MS.gene99009:CDS | 60.0% | |
| CGCTTTGCTGACCTATCGCG+TGG | + | 6917:24474-24493 | None:intergenic | 60.0% | |
| GCCTCCTATTTGTTCGGGGC+GGG | - | 6917:24864-24883 | MS.gene99009:CDS | 60.0% | |
| TCCTATTTGTTCGGGGCGGG+CGG | - | 6917:24867-24886 | MS.gene99009:CDS | 60.0% | |
| TCGTCGGGTGGTGCTTGTGT+GGG | - | 6917:24645-24664 | MS.gene99009:CDS | 60.0% | |
| TTCGTCGGGTGGTGCTTGTG+TGG | - | 6917:24644-24663 | MS.gene99009:CDS | 60.0% | |
| ! | AAATAGGAGGCGTGGGCCAC+AGG | + | 6917:24855-24874 | None:intergenic | 60.0% |
| !! | CAGCTGTCGGCTCGTTGGTA+AGG | + | 6917:24712-24731 | None:intergenic | 60.0% |
| AAGCACCACCCGACGAAGGG+GGG | + | 6917:24641-24660 | None:intergenic | 65.0% | |
| CAAGCACCACCCGACGAAGG+GGG | + | 6917:24642-24661 | None:intergenic | 65.0% | |
| CAGGTCGCACATAAGCCGCC+GGG | + | 6917:24836-24855 | None:intergenic | 65.0% | |
| CGTCGGGTGGTGCTTGTGTG+GGG | - | 6917:24646-24665 | MS.gene99009:CDS | 65.0% | |
| GCCCGCCCCGAACAAATAGG+AGG | + | 6917:24868-24887 | None:intergenic | 65.0% | |
| GGCGGCTTATGTGCGACCTG+TGG | - | 6917:24836-24855 | MS.gene99009:CDS | 65.0% | |
| GTCGGGTGGTGCTTGTGTGG+GGG | - | 6917:24647-24666 | MS.gene99009:CDS | 65.0% | |
| TCGGGTGGTGCTTGTGTGGG+GGG | - | 6917:24648-24667 | MS.gene99009:CDS | 65.0% | |
| TCTTCTGTCGTGCGACCCGG+CGG | - | 6917:24818-24837 | MS.gene99009:CDS | 65.0% | |
| TTTCCCCGTCCCCCCTTCGT+CGG | - | 6917:24629-24648 | MS.gene99009:CDS | 65.0% | |
| !! | GCCATCAGCTGTCGGCTCGT+TGG | + | 6917:24717-24736 | None:intergenic | 65.0% |
| ACCACCCGACGAAGGGGGGA+CGG | + | 6917:24637-24656 | None:intergenic | 70.0% | |
| CAGCAAAGCGTGAGCGGGCG+CGG | - | 6917:24484-24503 | MS.gene99009:CDS | 70.0% | |
| GCCGCCCGCCCCGAACAAAT+AGG | + | 6917:24871-24890 | None:intergenic | 70.0% | |
| GGGGGGACGGGGAAAGCTAC+AGG | + | 6917:24624-24643 | None:intergenic | 70.0% | |
| TTCCCCGTCCCCCCTTCGTC+GGG | - | 6917:24630-24649 | MS.gene99009:CDS | 70.0% | |
| !! | AAGGGTGCTGCCGTGCCACG+AGG | + | 6917:24099-24118 | None:intergenic | 70.0% |
| AGCGTGAGCGGGCGCGGAGA+AGG | - | 6917:24490-24509 | MS.gene99009:CDS | 75.0% | |
| CACCCGACGAAGGGGGGACG+GGG | + | 6917:24635-24654 | None:intergenic | 75.0% | |
| CCACCCGACGAAGGGGGGAC+GGG | + | 6917:24636-24655 | None:intergenic | 75.0% | |
| ! | CCCGTCCCCCCTTCGTCGGG+TGG | - | 6917:24633-24652 | MS.gene99009:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 6917 | gene | 24087 | 24944 | 24087 | ID=MS.gene99009 |
| 6917 | mRNA | 24087 | 24944 | 24087 | ID=MS.gene99009.t1;Parent=MS.gene99009 |
| 6917 | exon | 24087 | 24944 | 24087 | ID=MS.gene99009.t1.exon1;Parent=MS.gene99009.t1 |
| 6917 | CDS | 24087 | 24944 | 24087 | ID=cds.MS.gene99009.t1;Parent=MS.gene99009.t1 |
| Gene Sequence |
| Protein sequence |
