Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99427.t1 | XP_013459401.1 | 95.5 | 134 | 6 | 0 | 1 | 134 | 191 | 324 | 2.80E-63 | 251.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99427.t1 | A0A072UV00 | 95.5 | 134 | 6 | 0 | 1 | 134 | 191 | 324 | 2.0e-63 | 251.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052628 | MS.gene99427 | 0.819771 | 9.75E-53 | -1.69E-46 |
| MS.gene05894 | MS.gene99427 | 0.814118 | 1.81E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99427.t1 | MTR_3g037110 | 95.522 | 134 | 6 | 0 | 1 | 134 | 191 | 324 | 3.02e-91 | 267 |
| MS.gene99427.t1 | MTR_4g109020 | 64.925 | 134 | 41 | 1 | 1 | 134 | 197 | 324 | 2.24e-52 | 168 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99427.t1 | AT5G61340 | 47.581 | 124 | 62 | 2 | 13 | 133 | 202 | 325 | 4.90e-34 | 120 |
| MS.gene99427.t1 | AT5G61340 | 47.581 | 124 | 62 | 2 | 13 | 133 | 235 | 358 | 6.90e-34 | 121 |
Find 27 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAAGCATGTATTGTTCTA+AGG | 0.225162 | 3.4:-29209477 | MS.gene99427:CDS |
| AACATCAATGGCTTTGTTCT+TGG | 0.244052 | 3.4:-29209449 | MS.gene99427:CDS |
| TTGAAGCTTTGTTTCAATTC+CGG | 0.255839 | 3.4:-29209396 | MS.gene99427:CDS |
| ATAGAGGAGGACAGTTATTA+TGG | 0.314276 | 3.4:-29209184 | MS.gene99427:CDS |
| TGAAGCTTTGTTTCAATTCC+GGG | 0.319255 | 3.4:-29209395 | MS.gene99427:CDS |
| AACATGGCACTCACGCTATC+TGG | 0.373350 | 3.4:-29209532 | MS.gene99427:CDS |
| ACTTCCTGTTAATGTAGGTT+TGG | 0.385687 | 3.4:-29209425 | MS.gene99427:CDS |
| TTGGCACTTCCTGTTAATGT+AGG | 0.399443 | 3.4:-29209430 | MS.gene99427:CDS |
| GCAGCCAAACCTACATTAAC+AGG | 0.400186 | 3.4:+29209421 | None:intergenic |
| TACAAAAGCTGCAAAGTAAG+AGG | 0.421991 | 3.4:-29209253 | MS.gene99427:CDS |
| GGAGGGACATCGAGGTTATA+TGG | 0.427602 | 3.4:-29209506 | MS.gene99427:CDS |
| CTTGAAAATTCTTGATTCCC+AGG | 0.431044 | 3.4:+29209158 | None:intergenic |
| GGACAGTTATTATGGTTACC+TGG | 0.477672 | 3.4:-29209176 | MS.gene99427:CDS |
| GAAAGCATGTATTGTTCTAA+GGG | 0.478550 | 3.4:-29209476 | MS.gene99427:CDS |
| TTTCAATTCCGGGTCGTGAG+AGG | 0.505017 | 3.4:-29209385 | MS.gene99427:CDS |
| GAATTGAAACAAAGCTTCAA+TGG | 0.515494 | 3.4:+29209399 | None:intergenic |
| TTTGAGGATTGAACTCATAG+AGG | 0.542567 | 3.4:-29209200 | MS.gene99427:CDS |
| TTCAATTCCGGGTCGTGAGA+GGG | 0.542858 | 3.4:-29209384 | MS.gene99427:CDS |
| GACAGTTATTATGGTTACCT+GGG | 0.552491 | 3.4:-29209175 | MS.gene99427:CDS |
| ATTACATTGATGAAAAGTCA+TGG | 0.569559 | 3.4:-29209360 | MS.gene99427:CDS |
| TGTAATACCCTCTCACGACC+CGG | 0.576169 | 3.4:+29209377 | None:intergenic |
| GGCACTCACGCTATCTGGTG+TGG | 0.585973 | 3.4:-29209527 | MS.gene99427:CDS |
| GAGGATTGAACTCATAGAGG+AGG | 0.588877 | 3.4:-29209197 | MS.gene99427:CDS |
| ACTCACGCTATCTGGTGTGG+AGG | 0.597954 | 3.4:-29209524 | MS.gene99427:CDS |
| CTCACGCTATCTGGTGTGGA+GGG | 0.643335 | 3.4:-29209523 | MS.gene99427:CDS |
| AAGAGGATCATTGATTGACA+AGG | 0.665322 | 3.4:-29209236 | MS.gene99427:CDS |
| TCTGGTGTGGAGGGACATCG+AGG | 0.716596 | 3.4:-29209514 | MS.gene99427:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTACATTGATGAAAAGTCA+TGG | - | chr3.4:29209316-29209335 | MS.gene99427:CDS | 25.0% |
| GAATTGAAACAAAGCTTCAA+TGG | + | chr3.4:29209280-29209299 | None:intergenic | 30.0% | |
| TCTAAGGGAAAAAACATCAA+TGG | - | chr3.4:29209215-29209234 | MS.gene99427:CDS | 30.0% | |
| ! | GAAAGCATGTATTGTTCTAA+GGG | - | chr3.4:29209200-29209219 | MS.gene99427:CDS | 30.0% |
| ! | TGAAAGCATGTATTGTTCTA+AGG | - | chr3.4:29209199-29209218 | MS.gene99427:CDS | 30.0% |
| !! | TTGAAGCTTTGTTTCAATTC+CGG | - | chr3.4:29209280-29209299 | MS.gene99427:CDS | 30.0% |
| !!! | AGGAAGACAAACATTTTTTG+AGG | - | chr3.4:29209460-29209479 | MS.gene99427:CDS | 30.0% |
| ACTTCCTGTTAATGTAGGTT+TGG | - | chr3.4:29209251-29209270 | MS.gene99427:CDS | 35.0% | |
| ATAGAGGAGGACAGTTATTA+TGG | - | chr3.4:29209492-29209511 | MS.gene99427:CDS | 35.0% | |
| CTTGAAAATTCTTGATTCCC+AGG | + | chr3.4:29209521-29209540 | None:intergenic | 35.0% | |
| GACAGTTATTATGGTTACCT+GGG | - | chr3.4:29209501-29209520 | MS.gene99427:CDS | 35.0% | |
| TACAAAAGCTGCAAAGTAAG+AGG | - | chr3.4:29209423-29209442 | MS.gene99427:CDS | 35.0% | |
| TTTGAGGATTGAACTCATAG+AGG | - | chr3.4:29209476-29209495 | MS.gene99427:CDS | 35.0% | |
| ! | AAGAGGATCATTGATTGACA+AGG | - | chr3.4:29209440-29209459 | MS.gene99427:CDS | 35.0% |
| ! | GTCATGGTTTTTCATAGCTT+TGG | - | chr3.4:29209332-29209351 | MS.gene99427:CDS | 35.0% |
| !! | AACATCAATGGCTTTGTTCT+TGG | - | chr3.4:29209227-29209246 | MS.gene99427:CDS | 35.0% |
| !! | TGAAGCTTTGTTTCAATTCC+GGG | - | chr3.4:29209281-29209300 | MS.gene99427:CDS | 35.0% |
| !!! | ATGGTTTTTCATAGCTTTGG+AGG | - | chr3.4:29209335-29209354 | MS.gene99427:CDS | 35.0% |
| !!! | TGGTTTTTCATAGCTTTGGA+GGG | - | chr3.4:29209336-29209355 | MS.gene99427:CDS | 35.0% |
| GGACAGTTATTATGGTTACC+TGG | - | chr3.4:29209500-29209519 | MS.gene99427:CDS | 40.0% | |
| TTGGCACTTCCTGTTAATGT+AGG | - | chr3.4:29209246-29209265 | MS.gene99427:CDS | 40.0% | |
| GAGGATTGAACTCATAGAGG+AGG | - | chr3.4:29209479-29209498 | MS.gene99427:CDS | 45.0% | |
| GCAGCCAAACCTACATTAAC+AGG | + | chr3.4:29209258-29209277 | None:intergenic | 45.0% | |
| GGAGGGACATCGAGGTTATA+TGG | - | chr3.4:29209170-29209189 | MS.gene99427:CDS | 50.0% | |
| TGTAATACCCTCTCACGACC+CGG | + | chr3.4:29209302-29209321 | None:intergenic | 50.0% | |
| TTCAATTCCGGGTCGTGAGA+GGG | - | chr3.4:29209292-29209311 | MS.gene99427:CDS | 50.0% | |
| TTTCAATTCCGGGTCGTGAG+AGG | - | chr3.4:29209291-29209310 | MS.gene99427:CDS | 50.0% | |
| ACTCACGCTATCTGGTGTGG+AGG | - | chr3.4:29209152-29209171 | MS.gene99427:CDS | 55.0% | |
| CTCACGCTATCTGGTGTGGA+GGG | - | chr3.4:29209153-29209172 | MS.gene99427:CDS | 55.0% | |
| GGCACTCACGCTATCTGGTG+TGG | - | chr3.4:29209149-29209168 | MS.gene99427:CDS | 60.0% | |
| TCTGGTGTGGAGGGACATCG+AGG | - | chr3.4:29209162-29209181 | MS.gene99427:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 29209147 | 29209551 | 29209147 | ID=MS.gene99427 |
| chr3.4 | mRNA | 29209147 | 29209551 | 29209147 | ID=MS.gene99427.t1;Parent=MS.gene99427 |
| chr3.4 | exon | 29209147 | 29209551 | 29209147 | ID=MS.gene99427.t1.exon1;Parent=MS.gene99427.t1 |
| chr3.4 | CDS | 29209147 | 29209551 | 29209147 | ID=cds.MS.gene99427.t1;Parent=MS.gene99427.t1 |
| Gene Sequence |
| Protein sequence |
