Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99485.t1 | XP_024625759.1 | 96.5 | 930 | 30 | 2 | 1 | 930 | 1 | 927 | 0.00E+00 | 1771.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99485.t1 | O65020 | 68.4 | 957 | 262 | 7 | 9 | 933 | 1 | 949 | 0.0e+00 | 1280.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99485.t1 | G7KSD2 | 86.2 | 1035 | 35 | 4 | 1 | 930 | 1 | 1032 | 0.0e+00 | 1712.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene99485.t1 | TR | TRAF |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050762 | MS.gene99485 | 0.812851 | 3.44E-51 | -1.69E-46 |
| MS.gene050763 | MS.gene99485 | 0.808813 | 2.58E-50 | -1.69E-46 |
| MS.gene053486 | MS.gene99485 | 0.801409 | 9.13E-49 | -1.69E-46 |
| MS.gene054379 | MS.gene99485 | 0.817274 | 3.59E-52 | -1.69E-46 |
| MS.gene056480 | MS.gene99485 | 0.825206 | 5.32E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99485.t1 | MTR_4g014680 | 70.421 | 950 | 231 | 11 | 1 | 930 | 1 | 920 | 0.0 | 1317 |
| MS.gene99485.t1 | MTR_7g069700 | 94.362 | 674 | 33 | 3 | 1 | 672 | 1 | 671 | 0.0 | 1310 |
| MS.gene99485.t1 | MTR_7g069700 | 98.077 | 260 | 5 | 0 | 671 | 930 | 773 | 1032 | 6.59e-166 | 521 |
| MS.gene99485.t1 | MTR_7g069700 | 31.000 | 200 | 134 | 2 | 729 | 928 | 401 | 596 | 3.42e-20 | 97.8 |
| MS.gene99485.t1 | MTR_7g069700 | 30.978 | 184 | 127 | 0 | 416 | 599 | 847 | 1030 | 1.41e-19 | 95.9 |
| MS.gene99485.t1 | MTR_3g465430 | 59.465 | 935 | 349 | 10 | 9 | 933 | 1 | 915 | 0.0 | 1066 |
| MS.gene99485.t1 | MTR_1g071400 | 51.234 | 851 | 378 | 6 | 89 | 930 | 51 | 873 | 0.0 | 803 |
| MS.gene99485.t1 | MTR_1g071400 | 50.063 | 791 | 358 | 6 | 89 | 870 | 51 | 813 | 0.0 | 725 |
| MS.gene99485.t1 | MTR_1g071400 | 31.892 | 185 | 126 | 0 | 744 | 928 | 358 | 542 | 1.17e-20 | 99.0 |
| MS.gene99485.t1 | MTR_1g071400 | 46.734 | 689 | 330 | 6 | 89 | 768 | 51 | 711 | 0.0 | 575 |
| MS.gene99485.t1 | MTR_1g071400 | 31.892 | 185 | 126 | 0 | 744 | 928 | 358 | 542 | 5.30e-21 | 100 |
| MS.gene99485.t1 | MTR_7g069710 | 96.241 | 266 | 10 | 0 | 983 | 1248 | 1 | 266 | 0.0 | 536 |
| MS.gene99485.t1 | MTR_4g014670 | 48.410 | 283 | 94 | 14 | 991 | 1242 | 3 | 264 | 4.18e-53 | 187 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99485.t1 | AT3G51770 | 69.048 | 966 | 257 | 8 | 1 | 933 | 1 | 957 | 0.0 | 1334 |
| MS.gene99485.t1 | AT3G51770 | 69.102 | 958 | 254 | 8 | 9 | 933 | 1 | 949 | 0.0 | 1321 |
| MS.gene99485.t1 | AT5G58550 | 53.609 | 942 | 377 | 9 | 9 | 930 | 1 | 902 | 0.0 | 976 |
| MS.gene99485.t1 | AT4G02680 | 51.124 | 845 | 390 | 5 | 89 | 930 | 51 | 875 | 0.0 | 796 |
| MS.gene99485.t1 | AT3G51760 | 34.940 | 249 | 123 | 8 | 995 | 1222 | 16 | 246 | 3.98e-26 | 109 |
| MS.gene99485.t1 | AT3G51760 | 34.940 | 249 | 123 | 8 | 995 | 1222 | 16 | 246 | 3.98e-26 | 109 |
| MS.gene99485.t1 | AT3G51760 | 34.940 | 249 | 123 | 8 | 995 | 1222 | 16 | 246 | 3.98e-26 | 109 |
| MS.gene99485.t1 | AT3G51760 | 34.940 | 249 | 123 | 8 | 995 | 1222 | 7 | 237 | 7.90e-26 | 108 |
Find 272 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTAAGGCTAGTTTGGTTTC+AGG | 0.109106 | 7.1:-32430695 | MS.gene99485:CDS |
| CATAAGCTGATGCATTATTT+TGG | 0.125426 | 7.1:+32426940 | None:intergenic |
| TTGTAGGCACTCAATAATTT+AGG | 0.154747 | 7.1:-32427128 | MS.gene99485:intron |
| AGCTTCCAGTGTGGCTGTTT+TGG | 0.165102 | 7.1:+32431048 | None:intergenic |
| CCATAGAACATCATATAATT+TGG | 0.168779 | 7.1:+32429465 | None:intergenic |
| TCTGAAATCAATAAAATAAT+TGG | 0.192762 | 7.1:-32429594 | MS.gene99485:CDS |
| CCAAATTATATGATGTTCTA+TGG | 0.202581 | 7.1:-32429465 | MS.gene99485:CDS |
| AGATGTAACAGTTGCAATTC+TGG | 0.227845 | 7.1:+32425608 | None:intergenic |
| GATATTTACAAATATATTAT+TGG | 0.227898 | 7.1:-32425036 | MS.gene99485:CDS |
| GTTATTGAAGCGCTCTAATA+TGG | 0.235522 | 7.1:-32424474 | MS.gene99485:CDS |
| CGTTGATGATATTGGTTCTT+TGG | 0.244913 | 7.1:-32429343 | MS.gene99485:CDS |
| GCTTGGGACTTCTCTTCTCT+TGG | 0.256096 | 7.1:-32423809 | MS.gene99485:CDS |
| GGCTGCTTTGGTGTTGGATA+TGG | 0.256437 | 7.1:-32430258 | MS.gene99485:CDS |
| TCTCTTTGTATCAGTGTTAA+TGG | 0.257847 | 7.1:-32425687 | MS.gene99485:intron |
| AAAACAGCCCGGTGTTTATA+TGG | 0.268161 | 7.1:+32429651 | None:intergenic |
| AATACAAAGATGCACAAAAT+TGG | 0.273846 | 7.1:-32429899 | MS.gene99485:CDS |
| TTAAAGCTTGAAGAATTTAA+TGG | 0.273898 | 7.1:-32423993 | MS.gene99485:CDS |
| GCTAGTGGCAGCTTGCTTGC+AGG | 0.285640 | 7.1:-32430186 | MS.gene99485:CDS |
| CCCTTGCCGGCGTGCGTCGC+CGG | 0.286266 | 7.1:-32430943 | MS.gene99485:CDS |
| ACCTGTTGGTTGGATGTATC+AGG | 0.286915 | 7.1:-32429754 | MS.gene99485:CDS |
| CAAACATCACAACACCTAAT+TGG | 0.292783 | 7.1:+32429931 | None:intergenic |
| CCATACAACATTGTCATAAA+AGG | 0.296170 | 7.1:+32430476 | None:intergenic |
| CAACTTGCTTATCACCAATT+AGG | 0.297340 | 7.1:-32429945 | MS.gene99485:CDS |
| TGATGCTCATTTGGCTGCTT+TGG | 0.298085 | 7.1:-32430270 | MS.gene99485:CDS |
| CTTGGGACTTCTCTTCTCTT+GGG | 0.298615 | 7.1:-32423808 | MS.gene99485:CDS |
| AATCACGGGGTTTGTCTTAG+AGG | 0.304158 | 7.1:-32423873 | MS.gene99485:CDS |
| TGTGTGACCACGCTTATATT+TGG | 0.311310 | 7.1:+32429821 | None:intergenic |
| TGGTTCCACTTCTGAGCAAC+AGG | 0.313807 | 7.1:+32429405 | None:intergenic |
| CATCTCATCATATGCCGCTT+TGG | 0.316982 | 7.1:+32426983 | None:intergenic |
| ATCTCATCATATGCCGCTTT+GGG | 0.317455 | 7.1:+32426984 | None:intergenic |
| TCAACCCTCTCCGAGTCTTT+TGG | 0.317936 | 7.1:+32424281 | None:intergenic |
| GGACATATCCGTACAGATAT+AGG | 0.319554 | 7.1:-32426842 | MS.gene99485:CDS |
| GGGGATTGTTCCCATGAAAA+TGG | 0.320085 | 7.1:+32424311 | None:intergenic |
| AGTCCCAAGCCACATCCTTT+TGG | 0.320568 | 7.1:+32423822 | None:intergenic |
| GCGTTGTTGTTGATTGAGTA+TGG | 0.321993 | 7.1:-32430230 | MS.gene99485:CDS |
| GTATAGGTGATAGTGAAATT+AGG | 0.322217 | 7.1:-32430532 | MS.gene99485:CDS |
| ATCGGCTTGTGATGCTCATT+TGG | 0.324494 | 7.1:-32430279 | MS.gene99485:CDS |
| CAATTCTGGCTTAAAGTTGA+TGG | 0.331764 | 7.1:+32425622 | None:intergenic |
| TTTGGTTCGACCAGAAGTTT+TGG | 0.333972 | 7.1:+32431081 | None:intergenic |
| CAGGTTTAAGATCAGAAATA+AGG | 0.339519 | 7.1:+32429772 | None:intergenic |
| ACCAAGAGGTTGAGTCAGTT+TGG | 0.340955 | 7.1:-32430362 | MS.gene99485:CDS |
| CATGATCAATCGCTTTCTTC+CGG | 0.346815 | 7.1:-32423952 | MS.gene99485:CDS |
| AAAGAGTGTGATTGATCTCT+TGG | 0.347427 | 7.1:-32427301 | MS.gene99485:CDS |
| GATTTGGTTCAAACCCTTGC+CGG | 0.349062 | 7.1:-32430956 | MS.gene99485:CDS |
| CTAAGGCTAGTTTGGTTTCA+GGG | 0.356896 | 7.1:-32430694 | MS.gene99485:CDS |
| TCAGGGTGTGATCATGAATC+AGG | 0.357911 | 7.1:-32430677 | MS.gene99485:CDS |
| CGGAGAATCGGCGGCGGCGG+AGG | 0.358385 | 7.1:+32431171 | None:intergenic |
| TGAATGTCCTAAGGCTAGTT+TGG | 0.358860 | 7.1:-32430702 | MS.gene99485:CDS |
| CTAGACCGCTCCAAAACTTC+TGG | 0.365913 | 7.1:-32431091 | MS.gene99485:CDS |
| AAACTAAGATCGTTCTTTGC+CGG | 0.370718 | 7.1:+32426888 | None:intergenic |
| TTTGTAGAGCATGGAAGAAC+AGG | 0.377373 | 7.1:+32423753 | None:intergenic |
| GACATATCCGTACAGATATA+GGG | 0.383582 | 7.1:-32426841 | MS.gene99485:CDS |
| TTCTTGGAGGAGAATAAGAT+TGG | 0.384988 | 7.1:-32429627 | MS.gene99485:CDS |
| AAGCAATTGCAGAGCTTTCA+AGG | 0.386541 | 7.1:-32425646 | MS.gene99485:CDS |
| GGCAAAGAACGATCTTAGTT+TGG | 0.391087 | 7.1:-32426886 | MS.gene99485:CDS |
| TGGTCTTCCGTTGATGATAT+TGG | 0.396259 | 7.1:-32429351 | MS.gene99485:CDS |
| TTGTTTGAAGCCGTTTGATT+TGG | 0.402048 | 7.1:-32430972 | MS.gene99485:CDS |
| TGTAGGCACTCAATAATTTA+GGG | 0.405280 | 7.1:-32427127 | MS.gene99485:intron |
| CCAAGAGGTTGAGTCAGTTT+GGG | 0.407653 | 7.1:-32430361 | MS.gene99485:CDS |
| TCTCCAAAAGGATGTGGCTT+GGG | 0.408150 | 7.1:-32423825 | MS.gene99485:CDS |
| GGCTCCTTCATAATCCTTCA+TGG | 0.410046 | 7.1:+32429521 | None:intergenic |
| CTCTAATATGGAAGAAGATA+TGG | 0.410338 | 7.1:-32424462 | MS.gene99485:CDS |
| CAAGCATACACGAGCACATC+AGG | 0.413519 | 7.1:-32427039 | MS.gene99485:CDS |
| CTGTACGGATATGTCCTTAG+AGG | 0.414002 | 7.1:+32426849 | None:intergenic |
| GGCAATCTTTACTTCTGTTA+CGG | 0.416098 | 7.1:-32429266 | MS.gene99485:intron |
| TTTCTGATAGTAAATTGTTC+CGG | 0.416330 | 7.1:-32430859 | MS.gene99485:CDS |
| AGCCATGCGTAGTCTGCGTT+TGG | 0.420086 | 7.1:-32427508 | MS.gene99485:CDS |
| TCCTGATACATCCAACCAAC+AGG | 0.422721 | 7.1:+32429753 | None:intergenic |
| ATTTCTGATCTTAAACCTGT+TGG | 0.424740 | 7.1:-32429768 | MS.gene99485:CDS |
| CAACACAGTTGGATCCTCTA+AGG | 0.425094 | 7.1:-32426863 | MS.gene99485:CDS |
| TTGGTTCTTGATTGCCATGA+AGG | 0.431469 | 7.1:-32429535 | MS.gene99485:CDS |
| TCCTTGTGGCCTTCCTTCGT+CGG | 0.433566 | 7.1:-32431020 | MS.gene99485:CDS |
| TTGGTTTGAGGCTGCTGTTA+AGG | 0.434781 | 7.1:-32429880 | MS.gene99485:CDS |
| GTTAGTGTTGATAATGTTGA+TGG | 0.438872 | 7.1:-32430617 | MS.gene99485:CDS |
| CAAGACTTCCTCCATGCCTC+TGG | 0.440527 | 7.1:-32424229 | MS.gene99485:CDS |
| TGAAGATTGTGTTGGCTGCT+TGG | 0.442390 | 7.1:-32430793 | MS.gene99485:CDS |
| TCATGTGCTTCTTCTCCAAA+AGG | 0.444985 | 7.1:-32423837 | MS.gene99485:CDS |
| AACTAAGATCGTTCTTTGCC+GGG | 0.446592 | 7.1:+32426889 | None:intergenic |
| AGTGTTAATGGACGATCATA+AGG | 0.446891 | 7.1:-32425675 | MS.gene99485:CDS |
| CAGCATGCTGCCGCGCCGAC+CGG | 0.447684 | 7.1:+32430828 | None:intergenic |
| TTGGGAATGATCTTGTGCTT+GGG | 0.447922 | 7.1:-32430343 | MS.gene99485:CDS |
| AGAACCAAGCTCTCAATTCA+AGG | 0.447935 | 7.1:+32429550 | None:intergenic |
| AAGGAGCCCTTAGGGATGTC+AGG | 0.448282 | 7.1:-32429506 | MS.gene99485:CDS |
| TATATGGAAAAGTAAGAGTT+GGG | 0.449662 | 7.1:+32429667 | None:intergenic |
| TTTGGGAATGATCTTGTGCT+TGG | 0.451623 | 7.1:-32430344 | MS.gene99485:CDS |
| GCTCATAGTGACAGCAATGA+TGG | 0.451821 | 7.1:-32424023 | MS.gene99485:CDS |
| TGTAGAGGGTAGAGATAGAC+TGG | 0.452553 | 7.1:-32430102 | MS.gene99485:CDS |
| AATGTCCTTCAGATGGTCTT+CGG | 0.455974 | 7.1:-32427266 | MS.gene99485:CDS |
| AGGATTATGAAGGAGCCCTT+AGG | 0.458477 | 7.1:-32429515 | MS.gene99485:CDS |
| TTCTCCAAAAGGATGTGGCT+TGG | 0.458897 | 7.1:-32423826 | MS.gene99485:CDS |
| AAAGGGCTGCTTCACAGTCT+TGG | 0.459094 | 7.1:+32425540 | None:intergenic |
| TCCGGCGACGCACGCCGGCA+AGG | 0.459248 | 7.1:+32430942 | None:intergenic |
| ATCAACGCCGTCGTCGGCGG+TGG | 0.463982 | 7.1:-32431148 | MS.gene99485:CDS |
| CGCCGGATCGAGGGTGAGGG+TGG | 0.464077 | 7.1:-32430926 | MS.gene99485:CDS |
| GCTTGTTGGTTCTTCTGCAA+TGG | 0.465046 | 7.1:-32430744 | MS.gene99485:CDS |
| GCGTCGCCGGATCGAGGGTG+AGG | 0.467299 | 7.1:-32430930 | MS.gene99485:CDS |
| ACTGCCTTGAATTGAGAGCT+TGG | 0.469920 | 7.1:-32429554 | MS.gene99485:CDS |
| AATTGTTCCGGCGGAGTCTC+CGG | 0.469922 | 7.1:-32430847 | MS.gene99485:CDS |
| TTCGCCCTGTTGCTCAGAAG+TGG | 0.471523 | 7.1:-32429410 | MS.gene99485:CDS |
| AACTTCTGGTCGAACCAAAC+CGG | 0.474772 | 7.1:-32431077 | MS.gene99485:CDS |
| CAGAGGCATGGAGGAAGTCT+TGG | 0.475152 | 7.1:+32424230 | None:intergenic |
| TCCCACCCTCACCCTCGATC+CGG | 0.475722 | 7.1:+32430924 | None:intergenic |
| ATTCTTCAGTGGGCGTTGCA+AGG | 0.475946 | 7.1:-32429845 | MS.gene99485:CDS |
| GATCGGAGAATCGGCGGCGG+CGG | 0.476394 | 7.1:+32431168 | None:intergenic |
| GCAAGCACCAATGGGTCATT+AGG | 0.478574 | 7.1:+32425363 | None:intergenic |
| CCGGCGACGCACGCCGGCAA+GGG | 0.479079 | 7.1:+32430943 | None:intergenic |
| TCATTTGGCTGCTTTGGTGT+TGG | 0.479480 | 7.1:-32430264 | MS.gene99485:CDS |
| AGGTTTAAGATCAGAAATAA+GGG | 0.480132 | 7.1:+32429773 | None:intergenic |
| AAAGGTCTTTGTATTGCATC+GGG | 0.480166 | 7.1:-32429731 | MS.gene99485:CDS |
| TTGTGTTGGCTGCTTGGTTG+AGG | 0.481404 | 7.1:-32430787 | MS.gene99485:CDS |
| GTTGTTGATTGAGTATGGAT+TGG | 0.481475 | 7.1:-32430225 | MS.gene99485:CDS |
| GCTCTCAATTCAAGGCAGTC+AGG | 0.482894 | 7.1:+32429558 | None:intergenic |
| GGATTATGAAGGAGCCCTTA+GGG | 0.483017 | 7.1:-32429514 | MS.gene99485:CDS |
| TTGGGACTTCTCTTCTCTTG+GGG | 0.483194 | 7.1:-32423807 | MS.gene99485:CDS |
| CGGCGTGCGTCGCCGGATCG+AGG | 0.483201 | 7.1:-32430936 | MS.gene99485:CDS |
| AGATGGTCTTCGGAAGGGAC+AGG | 0.483338 | 7.1:-32427256 | MS.gene99485:intron |
| AGAAGAGAAGATGAGCTTGT+TGG | 0.485086 | 7.1:-32430758 | MS.gene99485:CDS |
| TTAGGAGAATGTGCGGAGAA+TGG | 0.489835 | 7.1:-32429978 | MS.gene99485:CDS |
| GAATATGAAAGGCGATCGTT+TGG | 0.490613 | 7.1:-32429442 | MS.gene99485:CDS |
| TATGCTTTGAATTGTGCTGA+TGG | 0.491781 | 7.1:+32423721 | None:intergenic |
| GATGACAAAGCTGATAGAAA+AGG | 0.492033 | 7.1:-32426964 | MS.gene99485:CDS |
| ATGGTGGATTTGTTGAGTCT+AGG | 0.494400 | 7.1:-32430457 | MS.gene99485:CDS |
| CTCGTGACAGCAAGCACCAA+TGG | 0.494881 | 7.1:+32425354 | None:intergenic |
| TTCTTCAGTGGGCGTTGCAA+GGG | 0.497679 | 7.1:-32429844 | MS.gene99485:CDS |
| AACGATTTCGACAAGAATCA+CGG | 0.498341 | 7.1:-32423888 | MS.gene99485:CDS |
| TGAGGTGATGAGGGCGGTTG+AGG | 0.499232 | 7.1:-32430396 | MS.gene99485:CDS |
| TCTTAGTTTGGCAACACAGT+TGG | 0.499800 | 7.1:-32426874 | MS.gene99485:CDS |
| CAATGCATTCGATTCTCTCT+AGG | 0.500136 | 7.1:-32424436 | MS.gene99485:CDS |
| AGAATATTGCGATCGTGACC+CGG | 0.500395 | 7.1:-32426907 | MS.gene99485:CDS |
| CCCAAACTGACTCAACCTCT+TGG | 0.501232 | 7.1:+32430361 | None:intergenic |
| GGCGTGCGTCGCCGGATCGA+GGG | 0.505772 | 7.1:-32430935 | MS.gene99485:CDS |
| GTTGATTGAGTATGGATTGG+AGG | 0.506035 | 7.1:-32430222 | MS.gene99485:CDS |
| CCTGAAACCAAACTAGCCTT+AGG | 0.506291 | 7.1:+32430695 | None:intergenic |
| CCGTACAGATATAGGGCAGC+AGG | 0.510354 | 7.1:-32426834 | MS.gene99485:intron |
| GAAAGGTCTTTGTATTGCAT+CGG | 0.511296 | 7.1:-32429732 | MS.gene99485:CDS |
| GACGGCGTTGATCGGAGAAT+CGG | 0.511486 | 7.1:+32431159 | None:intergenic |
| CCGCCGACGACGGCGTTGAT+CGG | 0.512881 | 7.1:+32431151 | None:intergenic |
| TGTTGCTCAGAAGTGGAACC+AGG | 0.514083 | 7.1:-32429403 | MS.gene99485:CDS |
| TCTTCCGGATGCTAATGCAT+TGG | 0.514286 | 7.1:-32423937 | MS.gene99485:CDS |
| AAGCGATTGATCATGTAAGT+TGG | 0.514575 | 7.1:+32423960 | None:intergenic |
| AGGGTTTGAACCAAATCAAA+CGG | 0.514627 | 7.1:+32430962 | None:intergenic |
| CTCAAACGTTGCATCGAGCT+AGG | 0.516884 | 7.1:+32430146 | None:intergenic |
| TTGTTGTACGGAGATGAAAT+CGG | 0.517052 | 7.1:-32430297 | MS.gene99485:CDS |
| CTCGATCCGGCGACGCACGC+CGG | 0.518205 | 7.1:+32430937 | None:intergenic |
| TGGAGCGGTCTAGTAAATGA+TGG | 0.518963 | 7.1:+32431101 | None:intergenic |
| GTGATGCTCTTAGAGAGGTT+AGG | 0.519954 | 7.1:-32429996 | MS.gene99485:CDS |
| GGACATATTTATTCTTCAGT+GGG | 0.519968 | 7.1:-32429855 | MS.gene99485:CDS |
| AGATGCACAAAATTGGTTTG+AGG | 0.521303 | 7.1:-32429892 | MS.gene99485:CDS |
| ATGGAGGAAGTCTTGGCCGA+GGG | 0.521326 | 7.1:+32424237 | None:intergenic |
| TCTCCGATCAACGCCGTCGT+CGG | 0.522127 | 7.1:-32431154 | MS.gene99485:CDS |
| GTTGAATCACAGGAGGGAAA+AGG | 0.523398 | 7.1:-32425291 | MS.gene99485:intron |
| GAGAATGTGCGGAGAATGGT+TGG | 0.526314 | 7.1:-32429974 | MS.gene99485:CDS |
| CATGAAAGACTTGTCTATGA+AGG | 0.526341 | 7.1:-32427462 | MS.gene99485:CDS |
| CTTCAAGCCTAATGACCCAT+TGG | 0.526452 | 7.1:-32425370 | MS.gene99485:intron |
| CAAATTATATGATGTTCTAT+GGG | 0.529449 | 7.1:-32429464 | MS.gene99485:CDS |
| TTTATTGGACGCCAGAGGCA+TGG | 0.529850 | 7.1:+32424218 | None:intergenic |
| CTGATCTTAAACCTGTTGGT+TGG | 0.530968 | 7.1:-32429764 | MS.gene99485:CDS |
| CTAGAAGCTCCGACGAAGGA+AGG | 0.534045 | 7.1:+32431011 | None:intergenic |
| CTGGAAGCTCTTCTTCCTTG+TGG | 0.535909 | 7.1:-32431034 | MS.gene99485:CDS |
| TTATATGGAAAAGTAAGAGT+TGG | 0.536160 | 7.1:+32429666 | None:intergenic |
| GCTTCTTCTCCAAAAGGATG+TGG | 0.537312 | 7.1:-32423831 | MS.gene99485:CDS |
| GTGATTCTTGTCGAAATCGT+TGG | 0.538181 | 7.1:+32423890 | None:intergenic |
| AATGGATTGTTGTGGAAGAA+AGG | 0.538901 | 7.1:-32430726 | MS.gene99485:CDS |
| CCCGGGCAAGCCAAAAGACT+CGG | 0.540457 | 7.1:-32424291 | MS.gene99485:CDS |
| AGTGGAACCAGGCTGATTGT+TGG | 0.540888 | 7.1:-32429392 | MS.gene99485:CDS |
| GAGATAGACTGGCTTGTGTA+GGG | 0.542301 | 7.1:-32430091 | MS.gene99485:CDS |
| AGGACATATTTATTCTTCAG+TGG | 0.544155 | 7.1:-32429856 | MS.gene99485:CDS |
| AAATTGCCCTGACATCCCTA+AGG | 0.545348 | 7.1:+32429499 | None:intergenic |
| AGTGAATTTGACAAGCAAGA+TGG | 0.546705 | 7.1:+32424350 | None:intergenic |
| CGTCGCCGGATCGAGGGTGA+GGG | 0.548713 | 7.1:-32430929 | MS.gene99485:CDS |
| CCGATCAACGCCGTCGTCGG+CGG | 0.548954 | 7.1:-32431151 | MS.gene99485:CDS |
| ATTGCCATGAAGGATTATGA+AGG | 0.549623 | 7.1:-32429525 | MS.gene99485:CDS |
| AGAGATAGACTGGCTTGTGT+AGG | 0.551278 | 7.1:-32430092 | MS.gene99485:CDS |
| TCCTTCAGATGGTCTTCGGA+AGG | 0.551380 | 7.1:-32427262 | MS.gene99485:CDS |
| ATGTTCTATGGGAATATGAA+AGG | 0.551432 | 7.1:-32429453 | MS.gene99485:CDS |
| GCAGCCAATGCATTAGCATC+CGG | 0.553590 | 7.1:+32423933 | None:intergenic |
| TCGTGACAGCAAGCACCAAT+GGG | 0.555162 | 7.1:+32425355 | None:intergenic |
| CTCAACTTCTTATGAAGATG+AGG | 0.555987 | 7.1:-32430582 | MS.gene99485:CDS |
| TTCTGTGCATTTGTAGAGCA+TGG | 0.557723 | 7.1:+32423744 | None:intergenic |
| TTTGAGTCAGGTTGCGATGG+AGG | 0.560395 | 7.1:-32430042 | MS.gene99485:CDS |
| AGGAGTCGATTTCTATTCAA+AGG | 0.560635 | 7.1:-32427389 | MS.gene99485:CDS |
| AAATCAAACGGCTTCAAACA+AGG | 0.560982 | 7.1:+32430974 | None:intergenic |
| CATGGAGGAAGTCTTGGCCG+AGG | 0.562418 | 7.1:+32424236 | None:intergenic |
| TTTGAGGCTGCTGTTAAGGA+AGG | 0.562936 | 7.1:-32429876 | MS.gene99485:CDS |
| TCGTGCTTCGGATCGAGGTG+AGG | 0.563792 | 7.1:+32431239 | None:intergenic |
| GAGTGTGATTGATCTCTTGG+AGG | 0.564967 | 7.1:-32427298 | MS.gene99485:CDS |
| TCTAACACCTCAGCATGGCT+AGG | 0.565229 | 7.1:+32425509 | None:intergenic |
| TTGCAGGTTAAATTCTCAGA+AGG | 0.567174 | 7.1:-32427532 | MS.gene99485:intron |
| ACGATTTCGACAAGAATCAC+GGG | 0.567217 | 7.1:-32423887 | MS.gene99485:CDS |
| ATGGCTAGGATCAAGACAAA+GGG | 0.568004 | 7.1:+32425523 | None:intergenic |
| AGAAGCTATAGCAAAGGCTG+AGG | 0.568097 | 7.1:-32427409 | MS.gene99485:CDS |
| TGGATTTGTTGAGTCTAGGA+GGG | 0.568303 | 7.1:-32430453 | MS.gene99485:CDS |
| GGTTCTAGAAGCTCCGACGA+AGG | 0.568479 | 7.1:+32431007 | None:intergenic |
| TGTGCATTTGAAGATTGTGT+TGG | 0.569827 | 7.1:-32430801 | MS.gene99485:CDS |
| AAAGACTTGTCTATGAAGGA+TGG | 0.571019 | 7.1:-32427458 | MS.gene99485:CDS |
| TTCAAACAAGGCTCAATCGA+AGG | 0.571307 | 7.1:+32430986 | None:intergenic |
| GTGGATTTGTTGAGTCTAGG+AGG | 0.573651 | 7.1:-32430454 | MS.gene99485:CDS |
| TGAGTATGGATTGGAGGAGA+TGG | 0.573672 | 7.1:-32430216 | MS.gene99485:CDS |
| CCTGCTGCCCTATATCTGTA+CGG | 0.576277 | 7.1:+32426834 | None:intergenic |
| CCTGTGATTCAACATCCAAA+AGG | 0.576549 | 7.1:+32425301 | None:intergenic |
| CTCATAGTGACAGCAATGAT+GGG | 0.576855 | 7.1:-32424022 | MS.gene99485:CDS |
| GTCTTTGTATTGCATCGGGA+AGG | 0.580398 | 7.1:-32429727 | MS.gene99485:CDS |
| GCGCCGACCGGAGACTCCGC+CGG | 0.580540 | 7.1:+32430840 | None:intergenic |
| GATCCGAAGCACGAAAATCA+CGG | 0.580778 | 7.1:-32431230 | MS.gene99485:CDS |
| AGCCAAACGCAGACTACGCA+TGG | 0.580853 | 7.1:+32427506 | None:intergenic |
| TTAGTGTTGATAATGTTGAT+GGG | 0.581210 | 7.1:-32430616 | MS.gene99485:CDS |
| TCTTCTGCAATGGATTGTTG+TGG | 0.581613 | 7.1:-32430734 | MS.gene99485:CDS |
| GTGACAGCAATGATGGGTTG+AGG | 0.581776 | 7.1:-32424016 | MS.gene99485:CDS |
| TTTATGACAATGTTGTATGG+TGG | 0.584334 | 7.1:-32430473 | MS.gene99485:CDS |
| GGAATCGCCACCGCCGACGA+CGG | 0.585594 | 7.1:+32431141 | None:intergenic |
| TGGACCCAAAACAGCCACAC+TGG | 0.586338 | 7.1:-32431053 | MS.gene99485:CDS |
| AAGAAAGGTTGAATGTCCTA+AGG | 0.586454 | 7.1:-32430711 | MS.gene99485:CDS |
| GCTCTTAAATGTCCTTCAGA+TGG | 0.589269 | 7.1:-32427273 | MS.gene99485:CDS |
| GGCGTTGATCGGAGAATCGG+CGG | 0.592552 | 7.1:+32431162 | None:intergenic |
| TCAAACGTTGCATCGAGCTA+GGG | 0.597450 | 7.1:+32430147 | None:intergenic |
| ATTGCATCCAACAATCAGCC+TGG | 0.597846 | 7.1:+32429385 | None:intergenic |
| GTGCTTGCTGTCACGAGGAA+AGG | 0.599977 | 7.1:-32425348 | MS.gene99485:CDS |
| ACTTCTTATGAAGATGAGGA+TGG | 0.600132 | 7.1:-32430578 | MS.gene99485:CDS |
| TCTGGTCGAACCAAACCGGT+TGG | 0.601522 | 7.1:-32431073 | MS.gene99485:CDS |
| AGAGAGGTTAGGAGAATGTG+CGG | 0.602272 | 7.1:-32429985 | MS.gene99485:CDS |
| CAGATGTTAGAAAATAACCC+GGG | 0.605466 | 7.1:-32429309 | MS.gene99485:CDS |
| CCAGATGTTAGAAAATAACC+CGG | 0.605861 | 7.1:-32429310 | MS.gene99485:CDS |
| GCCGGATCGAGGGTGAGGGT+GGG | 0.606510 | 7.1:-32430925 | MS.gene99485:CDS |
| AGCAATTGCAGAGCTTTCAA+GGG | 0.606547 | 7.1:-32425645 | MS.gene99485:CDS |
| TACACGAGCACATCAGGGGT+TGG | 0.606733 | 7.1:-32427033 | MS.gene99485:CDS |
| TTGGTTGGATGTATCAGGAA+AGG | 0.609801 | 7.1:-32429749 | MS.gene99485:CDS |
| CATGGCTAGGATCAAGACAA+AGG | 0.611388 | 7.1:+32425522 | None:intergenic |
| TCTTGATCCTAGCCATGCTG+AGG | 0.613827 | 7.1:-32425516 | MS.gene99485:intron |
| CATTGGTGCTTGCTGTCACG+AGG | 0.614718 | 7.1:-32425353 | MS.gene99485:CDS |
| TGGAGGAAGTCTTGGCCGAG+GGG | 0.617094 | 7.1:+32424238 | None:intergenic |
| CAAAGAACCAATATCATCAA+CGG | 0.617972 | 7.1:+32429344 | None:intergenic |
| AGGAGCCCTTAGGGATGTCA+GGG | 0.619291 | 7.1:-32429505 | MS.gene99485:CDS |
| GTTCCGGCGGAGTCTCCGGT+CGG | 0.620759 | 7.1:-32430843 | MS.gene99485:CDS |
| GATAAAGACGATGGTTGTGA+TGG | 0.625217 | 7.1:-32424152 | MS.gene99485:CDS |
| TCCGACGAAGGAAGGCCACA+AGG | 0.625348 | 7.1:+32431019 | None:intergenic |
| TTGGATGTTGAATCACAGGA+GGG | 0.625765 | 7.1:-32425297 | MS.gene99485:CDS |
| TTTGGATGTTGAATCACAGG+AGG | 0.626508 | 7.1:-32425298 | MS.gene99485:CDS |
| ACACGGGTCAGCTAATGCAT+AGG | 0.626512 | 7.1:+32427341 | None:intergenic |
| AAGCATACACGAGCACATCA+GGG | 0.626921 | 7.1:-32427038 | MS.gene99485:CDS |
| CCCTTCCGAAGACCATCTGA+AGG | 0.627028 | 7.1:+32427261 | None:intergenic |
| CTGATAGTAAATTGTTCCGG+CGG | 0.627378 | 7.1:-32430856 | MS.gene99485:CDS |
| CCTTCAGATGGTCTTCGGAA+GGG | 0.628021 | 7.1:-32427261 | MS.gene99485:CDS |
| TTGTAGAGCATGGAAGAACA+GGG | 0.630535 | 7.1:+32423754 | None:intergenic |
| AATTGCCCTGACATCCCTAA+GGG | 0.632546 | 7.1:+32429500 | None:intergenic |
| GCTTGTAGCAGTCAGCAGCA+AGG | 0.642893 | 7.1:+32427075 | None:intergenic |
| AAATTTCTAACACCTCAGCA+TGG | 0.643658 | 7.1:+32425504 | None:intergenic |
| TAGTGTTGATAATGTTGATG+GGG | 0.647542 | 7.1:-32430615 | MS.gene99485:CDS |
| GGCGGAGTCTCCGGTCGGCG+CGG | 0.649225 | 7.1:-32430838 | MS.gene99485:CDS |
| GGAGATGGCGTATTTGCTAG+TGG | 0.649477 | 7.1:-32430201 | MS.gene99485:CDS |
| TCGATGATGTAGAAACCCCT+CGG | 0.651455 | 7.1:-32424253 | MS.gene99485:CDS |
| CGACAGTGATGCTCTTAGAG+AGG | 0.651602 | 7.1:-32430001 | MS.gene99485:CDS |
| CGAAGCACGAAAATCACGGA+TGG | 0.654802 | 7.1:-32431226 | MS.gene99485:CDS |
| TCGTGAAGAAGCTATAGCAA+AGG | 0.655670 | 7.1:-32427415 | MS.gene99485:CDS |
| GGTTCCACTTCTGAGCAACA+GGG | 0.656342 | 7.1:+32429406 | None:intergenic |
| GAAGACTCTGAATCAAGACA+CGG | 0.658151 | 7.1:+32427324 | None:intergenic |
| CAAGCCAAAAGACTCGGAGA+GGG | 0.658942 | 7.1:-32424285 | MS.gene99485:CDS |
| GTTGATCGGAGAATCGGCGG+CGG | 0.659465 | 7.1:+32431165 | None:intergenic |
| TGATTGTTGGATGATGTCAA+AGG | 0.659868 | 7.1:+32424055 | None:intergenic |
| ATTGCATTTGATAAAGACGA+TGG | 0.661695 | 7.1:-32424161 | MS.gene99485:CDS |
| GCAAGCCAAAAGACTCGGAG+AGG | 0.662025 | 7.1:-32424286 | MS.gene99485:CDS |
| GGATGCAATTGTATGACAGA+TGG | 0.665267 | 7.1:-32429371 | MS.gene99485:CDS |
| GCAACTGTTACATCTTCGAG+CGG | 0.665843 | 7.1:-32425600 | MS.gene99485:CDS |
| CGATTTCGACAAGAATCACG+GGG | 0.670605 | 7.1:-32423886 | MS.gene99485:CDS |
| GGATGGATATTGTATGACAC+TGG | 0.671850 | 7.1:-32427441 | MS.gene99485:CDS |
| TTCTGTTGAGGTGATGAGGG+CGG | 0.682803 | 7.1:-32430402 | MS.gene99485:CDS |
| GCAAGGGCCAAATATAAGCG+TGG | 0.692048 | 7.1:-32429828 | MS.gene99485:CDS |
| AAGACTCTGAATCAAGACAC+GGG | 0.694198 | 7.1:+32427325 | None:intergenic |
| ATTGGACGCCAGAGGCATGG+AGG | 0.698776 | 7.1:+32424221 | None:intergenic |
| TCTTCAACGTCAGCCCAAAG+CGG | 0.707078 | 7.1:-32426997 | MS.gene99485:CDS |
| AGCATACACGAGCACATCAG+GGG | 0.713965 | 7.1:-32427037 | MS.gene99485:CDS |
| AGGAAGAAGAGCTTCCAGTG+TGG | 0.736071 | 7.1:+32431039 | None:intergenic |
| AGATGTTAGAAAATAACCCG+GGG | 0.738492 | 7.1:-32429308 | MS.gene99485:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 32423677 | 32431275 | 32423677 | ID=MS.gene99485 |
| chr7.1 | mRNA | 32423677 | 32431275 | 32423677 | ID=MS.gene99485.t1;Parent=MS.gene99485 |
| chr7.1 | exon | 32429267 | 32431275 | 32429267 | ID=MS.gene99485.t1.exon1;Parent=MS.gene99485.t1 |
| chr7.1 | CDS | 32429267 | 32431275 | 32429267 | ID=cds.MS.gene99485.t1;Parent=MS.gene99485.t1 |
| chr7.1 | exon | 32427257 | 32427548 | 32427257 | ID=MS.gene99485.t1.exon2;Parent=MS.gene99485.t1 |
| chr7.1 | CDS | 32427257 | 32427548 | 32427257 | ID=cds.MS.gene99485.t1;Parent=MS.gene99485.t1 |
| chr7.1 | exon | 32426835 | 32427144 | 32426835 | ID=MS.gene99485.t1.exon3;Parent=MS.gene99485.t1 |
| chr7.1 | CDS | 32426835 | 32427144 | 32426835 | ID=cds.MS.gene99485.t1;Parent=MS.gene99485.t1 |
| chr7.1 | exon | 32425517 | 32425695 | 32425517 | ID=MS.gene99485.t1.exon4;Parent=MS.gene99485.t1 |
| chr7.1 | CDS | 32425517 | 32425695 | 32425517 | ID=cds.MS.gene99485.t1;Parent=MS.gene99485.t1 |
| chr7.1 | exon | 32425292 | 32425385 | 32425292 | ID=MS.gene99485.t1.exon5;Parent=MS.gene99485.t1 |
| chr7.1 | CDS | 32425292 | 32425385 | 32425292 | ID=cds.MS.gene99485.t1;Parent=MS.gene99485.t1 |
| chr7.1 | exon | 32425020 | 32425063 | 32425020 | ID=MS.gene99485.t1.exon6;Parent=MS.gene99485.t1 |
| chr7.1 | CDS | 32425020 | 32425063 | 32425020 | ID=cds.MS.gene99485.t1;Parent=MS.gene99485.t1 |
| chr7.1 | exon | 32423677 | 32424495 | 32423677 | ID=MS.gene99485.t1.exon7;Parent=MS.gene99485.t1 |
| chr7.1 | CDS | 32423677 | 32424495 | 32423677 | ID=cds.MS.gene99485.t1;Parent=MS.gene99485.t1 |
| Gene Sequence |
| Protein sequence |
