Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99950.t1 | XP_013465630.1 | 100 | 91 | 0 | 0 | 1 | 91 | 246 | 336 | 1.80E-45 | 191.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99950.t1 | Q6ETL2 | 59.1 | 88 | 35 | 1 | 5 | 91 | 307 | 394 | 1.1e-24 | 113.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99950.t1 | G7ZYZ4 | 100.0 | 91 | 0 | 0 | 1 | 91 | 246 | 336 | 1.3e-45 | 191.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene99950.t1 | TF | TUB |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049418 | MS.gene99950 | 0.804926 | 1.71E-49 | -1.69E-46 |
| MS.gene058196 | MS.gene99950 | 0.803086 | 4.12E-49 | -1.69E-46 |
| MS.gene058197 | MS.gene99950 | 0.821634 | 3.64E-53 | -1.69E-46 |
| MS.gene058198 | MS.gene99950 | 0.821691 | 3.53E-53 | -1.69E-46 |
| MS.gene059300 | MS.gene99950 | 0.813034 | 3.14E-51 | -1.69E-46 |
| MS.gene06057 | MS.gene99950 | 0.809434 | 1.90E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99950.t1 | MTR_2g105390 | 100.000 | 91 | 0 | 0 | 1 | 91 | 246 | 336 | 7.62e-63 | 194 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99950.t1 | AT1G16070 | 56.322 | 87 | 38 | 0 | 5 | 91 | 286 | 372 | 1.09e-27 | 103 |
| MS.gene99950.t1 | AT1G16070 | 55.882 | 68 | 30 | 0 | 1 | 68 | 282 | 349 | 5.73e-18 | 77.0 |
Find 25 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTCAGGTACAACACATTA+AGG | 0.279223 | 2.1:+565362 | MS.gene99950:intron |
| AAACTTTCAGCTCACGTTAG+AGG | 0.301321 | 2.1:+565630 | MS.gene99950:CDS |
| GAGCTTGACTATAGAGATAA+AGG | 0.329596 | 2.1:+565574 | MS.gene99950:CDS |
| TTAAGGGACTGCCAATGAAT+TGG | 0.380340 | 2.1:+565379 | MS.gene99950:CDS |
| TTCCTACCCTCCCTAGCTGC+AGG | 0.422680 | 2.1:-565770 | None:intergenic |
| TAAGGGACTGCCAATGAATT+GGG | 0.444518 | 2.1:+565380 | MS.gene99950:CDS |
| GTTTGCTATGATTAGGAGAA+TGG | 0.459646 | 2.1:+565735 | MS.gene99950:intron |
| GATAAAGGAAGGCCAGGACT+TGG | 0.466820 | 2.1:+565589 | MS.gene99950:CDS |
| AGCAGACAATCCTGCAGCTA+GGG | 0.521878 | 2.1:+565760 | MS.gene99950:CDS |
| CCTCCTACCTTGTTATAGAG+TGG | 0.527819 | 2.1:-565444 | None:intergenic |
| TATAGAGATAAAGGAAGGCC+AGG | 0.527875 | 2.1:+565583 | MS.gene99950:CDS |
| AAGCAGACAATCCTGCAGCT+AGG | 0.533935 | 2.1:+565759 | MS.gene99950:CDS |
| TTTGCTATGATTAGGAGAAT+GGG | 0.539684 | 2.1:+565736 | MS.gene99950:intron |
| AAGTGCATCAACTATTCTCA+AGG | 0.551216 | 2.1:+565418 | MS.gene99950:CDS |
| GTTCAGGTACAACACATTAA+GGG | 0.551230 | 2.1:+565363 | MS.gene99950:intron |
| GATCTCTGGATTCCAAGTCC+TGG | 0.553307 | 2.1:-565601 | None:intergenic |
| AGACAATCCTGCAGCTAGGG+AGG | 0.560928 | 2.1:+565763 | MS.gene99950:CDS |
| GTCTTTCAAGAACACCAACC+AGG | 0.561985 | 2.1:+565236 | MS.gene99950:CDS |
| GACAATCCTGCAGCTAGGGA+GGG | 0.574505 | 2.1:+565764 | MS.gene99950:CDS |
| ATGAATCTAACTAACCTGGT+TGG | 0.618468 | 2.1:-565250 | None:intergenic |
| ATCCTGCAGCTAGGGAGGGT+AGG | 0.625245 | 2.1:+565768 | MS.gene99950:CDS |
| TTGACTATAGAGATAAAGGA+AGG | 0.630332 | 2.1:+565578 | MS.gene99950:CDS |
| GGACTGCCAATGAATTGGGA+AGG | 0.634626 | 2.1:+565384 | MS.gene99950:CDS |
| AGTGCATCAACTATTCTCAA+GGG | 0.640841 | 2.1:+565419 | MS.gene99950:CDS |
| AAGGGATCCACTCTATAACA+AGG | 0.674607 | 2.1:+565437 | MS.gene99950:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTACTAACAATAAAATTT+GGG | + | chr2.1:565340-565359 | MS.gene99950:intron | 10.0% |
| !! | TTATTACTAACAATAAAATT+TGG | + | chr2.1:565339-565358 | MS.gene99950:intron | 10.0% |
| !! | ATTACTAACAATAAAATTTG+GGG | + | chr2.1:565341-565360 | MS.gene99950:intron | 15.0% |
| !! | TTAATGAAAATCAATATCAA+TGG | - | chr2.1:565515-565534 | None:intergenic | 15.0% |
| !!! | AGGAAAATCTAATTTTGTTA+TGG | + | chr2.1:565788-565807 | MS.gene99950:CDS | 20.0% |
| !!! | GTTTTTATGTTATAACAACT+CGG | + | chr2.1:565682-565701 | MS.gene99950:intron | 20.0% |
| ! | TTATAACATAAAAACCCTTC+CGG | - | chr2.1:565677-565696 | None:intergenic | 25.0% |
| AATGAATTGGGAAGGAAAAA+TGG | + | chr2.1:565392-565411 | MS.gene99950:CDS | 30.0% | |
| GAAAATCAATATCAATGGCT+TGG | - | chr2.1:565510-565529 | None:intergenic | 30.0% | |
| GAAAATGAATCTAACTAACC+TGG | - | chr2.1:565257-565276 | None:intergenic | 30.0% | |
| TATAACATAAAAACCCTTCC+GGG | - | chr2.1:565676-565695 | None:intergenic | 30.0% | |
| TTGACTATAGAGATAAAGGA+AGG | + | chr2.1:565578-565597 | MS.gene99950:CDS | 30.0% | |
| ! | AACAATAAAATTTGGGGTTC+AGG | + | chr2.1:565347-565366 | MS.gene99950:intron | 30.0% |
| ! | ATTTTTCCTTCCCAATTCAT+TGG | - | chr2.1:565393-565412 | None:intergenic | 30.0% |
| ! | TTTGCTATGATTAGGAGAAT+GGG | + | chr2.1:565736-565755 | MS.gene99950:intron | 30.0% |
| !! | GATTTGTGTTTGCTATGATT+AGG | + | chr2.1:565728-565747 | MS.gene99950:intron | 30.0% |
| AAGTGCATCAACTATTCTCA+AGG | + | chr2.1:565418-565437 | MS.gene99950:CDS | 35.0% | |
| AGTGCATCAACTATTCTCAA+GGG | + | chr2.1:565419-565438 | MS.gene99950:CDS | 35.0% | |
| ATGAATCTAACTAACCTGGT+TGG | - | chr2.1:565253-565272 | None:intergenic | 35.0% | |
| GAGCTTGACTATAGAGATAA+AGG | + | chr2.1:565574-565593 | MS.gene99950:CDS | 35.0% | |
| GTTCAGGTACAACACATTAA+GGG | + | chr2.1:565363-565382 | MS.gene99950:intron | 35.0% | |
| TTGCTTTGTACTCTTGATGA+AGG | - | chr2.1:565551-565570 | None:intergenic | 35.0% | |
| ! | CGTGAGCTGAAAGTTTTTAA+CGG | - | chr2.1:565626-565645 | None:intergenic | 35.0% |
| !! | GAAAGTTTTTAACGGATCTC+TGG | - | chr2.1:565618-565637 | None:intergenic | 35.0% |
| !! | GTTTGCTATGATTAGGAGAA+TGG | + | chr2.1:565735-565754 | MS.gene99950:intron | 35.0% |
| AAACTTTCAGCTCACGTTAG+AGG | + | chr2.1:565630-565649 | MS.gene99950:CDS | 40.0% | |
| AAAGAAACTTCCCCTAAGTG+AGG | - | chr2.1:565472-565491 | None:intergenic | 40.0% | |
| AAGGGATCCACTCTATAACA+AGG | + | chr2.1:565437-565456 | MS.gene99950:CDS | 40.0% | |
| GATCCACTCTATAACAAGGT+AGG | + | chr2.1:565441-565460 | MS.gene99950:intron | 40.0% | |
| GGTTCAGGTACAACACATTA+AGG | + | chr2.1:565362-565381 | MS.gene99950:intron | 40.0% | |
| TAAGGGACTGCCAATGAATT+GGG | + | chr2.1:565380-565399 | MS.gene99950:CDS | 40.0% | |
| TATAGAGATAAAGGAAGGCC+AGG | + | chr2.1:565583-565602 | MS.gene99950:CDS | 40.0% | |
| TTAAGGGACTGCCAATGAAT+TGG | + | chr2.1:565379-565398 | MS.gene99950:CDS | 40.0% | |
| AGAGGTAACATCATTGACGC+CGG | + | chr2.1:565648-565667 | MS.gene99950:intron | 45.0% | |
| ATAAAAACCCTTCCGGGTTC+CGG | - | chr2.1:565670-565689 | None:intergenic | 45.0% | |
| CCACTCTATAACAAGGTAGG+AGG | + | chr2.1:565444-565463 | MS.gene99950:intron | 45.0% | |
| GTCTTTCAAGAACACCAACC+AGG | + | chr2.1:565236-565255 | MS.gene99950:CDS | 45.0% | |
| ! | CCTCCTACCTTGTTATAGAG+TGG | - | chr2.1:565447-565466 | None:intergenic | 45.0% |
| AAGCAGACAATCCTGCAGCT+AGG | + | chr2.1:565759-565778 | MS.gene99950:CDS | 50.0% | |
| AGCAGACAATCCTGCAGCTA+GGG | + | chr2.1:565760-565779 | MS.gene99950:CDS | 50.0% | |
| GATAAAGGAAGGCCAGGACT+TGG | + | chr2.1:565589-565608 | MS.gene99950:CDS | 50.0% | |
| GATCTCTGGATTCCAAGTCC+TGG | - | chr2.1:565604-565623 | None:intergenic | 50.0% | |
| GGACTGCCAATGAATTGGGA+AGG | + | chr2.1:565384-565403 | MS.gene99950:CDS | 50.0% | |
| ACATCATTGACGCCGGAACC+CGG | + | chr2.1:565655-565674 | MS.gene99950:intron | 55.0% | |
| AGACAATCCTGCAGCTAGGG+AGG | + | chr2.1:565763-565782 | MS.gene99950:CDS | 55.0% | |
| AGGTAGGAGGAGCCTCACTT+AGG | + | chr2.1:565457-565476 | MS.gene99950:intron | 55.0% | |
| GACAATCCTGCAGCTAGGGA+GGG | + | chr2.1:565764-565783 | MS.gene99950:CDS | 55.0% | |
| GGTAGGAGGAGCCTCACTTA+GGG | + | chr2.1:565458-565477 | MS.gene99950:intron | 55.0% | |
| GTAGGAGGAGCCTCACTTAG+GGG | + | chr2.1:565459-565478 | MS.gene99950:intron | 55.0% | |
| ATCCTGCAGCTAGGGAGGGT+AGG | + | chr2.1:565768-565787 | MS.gene99950:CDS | 60.0% | |
| ATTGACGCCGGAACCCGGAA+GGG | + | chr2.1:565660-565679 | MS.gene99950:intron | 60.0% | |
| TTCCTACCCTCCCTAGCTGC+AGG | - | chr2.1:565773-565792 | None:intergenic | 60.0% | |
| CATTGACGCCGGAACCCGGA+AGG | + | chr2.1:565659-565678 | MS.gene99950:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 565234 | 565817 | 565234 | ID=MS.gene99950 |
| chr2.1 | mRNA | 565234 | 565817 | 565234 | ID=MS.gene99950.t1;Parent=MS.gene99950 |
| chr2.1 | exon | 565234 | 565257 | 565234 | ID=MS.gene99950.t1.exon1;Parent=MS.gene99950.t1 |
| chr2.1 | CDS | 565234 | 565257 | 565234 | ID=cds.MS.gene99950.t1;Parent=MS.gene99950.t1 |
| chr2.1 | exon | 565369 | 565458 | 565369 | ID=MS.gene99950.t1.exon2;Parent=MS.gene99950.t1 |
| chr2.1 | CDS | 565369 | 565458 | 565369 | ID=cds.MS.gene99950.t1;Parent=MS.gene99950.t1 |
| chr2.1 | exon | 565559 | 565651 | 565559 | ID=MS.gene99950.t1.exon3;Parent=MS.gene99950.t1 |
| chr2.1 | CDS | 565559 | 565651 | 565559 | ID=cds.MS.gene99950.t1;Parent=MS.gene99950.t1 |
| chr2.1 | exon | 565750 | 565817 | 565750 | ID=MS.gene99950.t1.exon4;Parent=MS.gene99950.t1 |
| chr2.1 | CDS | 565750 | 565817 | 565750 | ID=cds.MS.gene99950.t1;Parent=MS.gene99950.t1 |
| Gene Sequence |
| Protein sequence |
