AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048006.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048006.01.T01 MTR_3g078090 59.631 379 45 6 55 325 166 544 1.55e-121 363
MsG0080048006.01.T01 MTR_8g064560 31.412 347 139 7 58 325 217 543 3.30e-38 144
MsG0080048006.01.T01 MTR_8g064560 36.000 175 79 4 62 204 165 338 6.24e-11 63.9
MsG0080048006.01.T01 MTR_5g034320 37.456 283 146 6 62 325 276 546 8.18e-38 143
MsG0080048006.01.T01 MTR_8g064530 34.426 305 153 6 62 325 262 560 4.91e-37 141
MsG0080048006.01.T01 MTR_8g064530 29.255 188 100 3 49 204 165 351 1.01e-12 69.3
MsG0080048006.01.T01 MTR_8g064500 54.902 102 46 0 224 325 189 290 6.72e-28 112
MsG0080048006.01.T01 MTR_4g133840 49.515 103 36 2 224 325 19 106 1.25e-23 96.7
MsG0080048006.01.T01 MTR_5g033350 38.835 103 62 1 224 325 17 119 9.68e-15 72.4
MsG0080048006.01.T01 MTR_5g033340 38.835 103 62 1 224 325 196 298 7.57e-14 72.4
MsG0080048006.01.T01 MTR_5g033340 47.368 76 40 0 250 325 15 90 8.30e-14 72.0
MsG0080048006.01.T01 MTR_5g033330 38.835 103 62 1 224 325 227 329 1.28e-13 72.4
MsG0080048006.01.T01 MTR_5g033330 38.835 103 62 1 224 325 435 537 1.28e-13 72.4
MsG0080048006.01.T01 MTR_5g033330 37.864 103 63 1 224 325 19 121 5.81e-13 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048006.01.T01 AT1G70370 39.493 276 142 5 62 325 280 542 9.74e-45 162
MsG0080048006.01.T01 AT1G70370 39.493 276 142 5 62 325 280 542 9.74e-45 162
MsG0080048006.01.T01 AT1G23760 38.014 292 139 8 59 325 264 538 7.41e-41 151
MsG0080048006.01.T01 AT1G60390 34.545 330 142 7 58 325 223 540 8.72e-40 148

Find 72 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGATTGGGACGCGCGTTTA+AGG 0.171525 contig168end:+3931 MsG0080048006.01.T01:CDS
TGACAATAATAGAGTAAATA+AGG 0.192990 contig168end:-4460 MsG0080048006.01.T01:intergenic
AATGACACTTTCACTGAATA+TGG 0.237140 contig168end:+3866 MsG0080048006.01.T01:CDS
TACGGCACTGGTTCCGTTTC+CGG 0.237545 contig168end:+3717 MsG0080048006.01.T01:CDS
GTGTTTGGATTCAAAACTTA+TGG 0.265235 contig168end:+3911 MsG0080048006.01.T01:CDS
TACGGTTCCGGTTCAAATTC+CGG 0.266445 contig168end:+3378 MsG0080048006.01.T01:CDS
TTCACCGGTTACGGTGAATT+AGG 0.291344 contig168end:+3622 MsG0080048006.01.T01:CDS
GTAAGCTTTGAAACTGCTTT+TGG 0.303933 contig168end:-3697 MsG0080048006.01.T01:intergenic
GGCCGGGGACAACAAATTTA+TGG 0.307216 contig168end:-2564 MsG0080048006.01.T01:intergenic
GCTTCATAAATCTCACTTTA+GGG 0.314239 contig168end:-4489 MsG0080048006.01.T01:intergenic
CTCAACCTCGAGGATGAAAA+TGG 0.315748 contig168end:-4152 MsG0080048006.01.T01:intergenic
AGCTTCATAAATCTCACTTT+AGG 0.339336 contig168end:-4490 MsG0080048006.01.T01:intergenic
AAAATGTGAACGGGTCAAAA+AGG 0.347765 contig168end:+4360 MsG0080048006.01.T01:CDS
GGATTCAAAACTTATGGATT+GGG 0.389201 contig168end:+3917 MsG0080048006.01.T01:CDS
GCTACGGGTAAAACCGTGTT+TGG 0.389321 contig168end:+3896 MsG0080048006.01.T01:CDS
CAATCAACATTCACCGGTTA+CGG 0.396679 contig168end:+3613 MsG0080048006.01.T01:CDS
TGTTCCTAATTCACCGTAAC+CGG 0.405892 contig168end:-3626 MsG0080048006.01.T01:intergenic
TGGATTCAAAACTTATGGAT+TGG 0.408522 contig168end:+3916 MsG0080048006.01.T01:CDS
GACCATAAATTTGTTGTCCC+CGG 0.423065 contig168end:+2562 MsG0080048006.01.T01:CDS
ATAAGGTAAAACGACCTTGC+AGG 0.430573 contig168end:-4112 MsG0080048006.01.T01:intergenic
AACTTCACAAACTACGGTTC+CGG 0.437081 contig168end:+3366 MsG0080048006.01.T01:CDS
AAAAGCAGTTTCAAAGCTTA+CGG 0.452353 contig168end:+3699 MsG0080048006.01.T01:CDS
AGTTTCAAAGCTTACGGCAC+TGG 0.467719 contig168end:+3705 MsG0080048006.01.T01:CDS
AAAAGAATCGGAGCCGGAAA+CGG 0.470860 contig168end:-3730 MsG0080048006.01.T01:intergenic
TCCGGTTCAAATTCCGGCAC+CGG 0.477147 contig168end:+3384 MsG0080048006.01.T01:CDS
GAATAATTTGTGATTGTTAG+CGG 0.478709 contig168end:-3472 MsG0080048006.01.T01:intergenic
TCCGGTGCCGGAATTTGAAC+CGG 0.489824 contig168end:-3385 MsG0080048006.01.T01:intergenic
GAATGTTGATTGAAGAATGT+TGG 0.499804 contig168end:-3602 MsG0080048006.01.T01:intergenic
TTCAACAGCTACGCAGAAAA+CGG 0.500011 contig168end:+3327 MsG0080048006.01.T01:CDS
AAAAGAAGCGTTTCACGCGC+GGG 0.501776 contig168end:+4182 MsG0080048006.01.T01:CDS
GACAATAATAGAGTAAATAA+GGG 0.503977 contig168end:-4459 MsG0080048006.01.T01:intergenic
ATTCTTCAATCAACATTCAC+CGG 0.504853 contig168end:+3607 MsG0080048006.01.T01:CDS
CCGGTAACTTACAAAAGAAT+CGG 0.507346 contig168end:-3742 MsG0080048006.01.T01:intergenic
AAACAACTGAAAATGTGAAC+GGG 0.509569 contig168end:+4351 MsG0080048006.01.T01:CDS
AATTATTCATTCTCAAGCTA+CGG 0.514141 contig168end:+3487 MsG0080048006.01.T01:CDS
AACACAAACTTCACAAACTA+CGG 0.525394 contig168end:+3360 MsG0080048006.01.T01:CDS
ATGCGAGAGGGAACCTAGTG+TGG 0.528725 contig168end:+4247 MsG0080048006.01.T01:CDS
ATTGTTAGCGGCGGAGTCGT+AGG 0.531751 contig168end:-3460 MsG0080048006.01.T01:intergenic
TTCAAAGCGTACAGTTTCAA+CGG 0.534195 contig168end:+3667 MsG0080048006.01.T01:CDS
TAGGTGGTGAAACCGAGGTT+GGG 0.534668 contig168end:-3441 MsG0080048006.01.T01:intergenic
ACTTTCACTGAATATGGAAA+AGG 0.539985 contig168end:+3872 MsG0080048006.01.T01:CDS
GAATATGGAAAAGGAGCTAC+GGG 0.539985 contig168end:+3881 MsG0080048006.01.T01:CDS
GGTTACGGTGAATTAGGAAC+AGG 0.550847 contig168end:+3628 MsG0080048006.01.T01:CDS
GTTGGGACCTAATATAGAAA+CGG 0.552509 contig168end:-4318 MsG0080048006.01.T01:intergenic
TGAATATGGAAAAGGAGCTA+CGG 0.559278 contig168end:+3880 MsG0080048006.01.T01:CDS
CTCGTTAATGTACCCAACCT+CGG 0.580215 contig168end:+3429 MsG0080048006.01.T01:CDS
GAAGCGTTTCACGCGCGGGA+TGG 0.581260 contig168end:+4186 MsG0080048006.01.T01:CDS
GGAGAAACAAAACGGTGCGT+TGG 0.581963 contig168end:+4269 MsG0080048006.01.T01:CDS
TAATTTCGATGATAGAGATA+AGG 0.596568 contig168end:-4129 MsG0080048006.01.T01:intergenic
AAAGTCGATGCAACGGACGG+TGG 0.596657 contig168end:+4398 MsG0080048006.01.T01:CDS
GGAAAAGTCGATGCAACGGA+CGG 0.604051 contig168end:+4395 MsG0080048006.01.T01:CDS
CGGTGCGTTGGATCAGCTGA+GGG 0.613492 contig168end:+4281 MsG0080048006.01.T01:CDS
ACTTACAAAAGAATCGGAGC+CGG 0.614704 contig168end:-3736 MsG0080048006.01.T01:intergenic
GTAGGTGGTGAAACCGAGGT+TGG 0.619572 contig168end:-3442 MsG0080048006.01.T01:intergenic
CTAGTGTGGGAGAAACAAAA+CGG 0.619596 contig168end:+4261 MsG0080048006.01.T01:CDS
TGCGAGAGGGAACCTAGTGT+GGG 0.624114 contig168end:+4248 MsG0080048006.01.T01:CDS
ACGGTGCGTTGGATCAGCTG+AGG 0.635487 contig168end:+4280 MsG0080048006.01.T01:CDS
AAAACAACTGAAAATGTGAA+CGG 0.637468 contig168end:+4350 MsG0080048006.01.T01:CDS
AGAGAGCGACAAGAACAGGA+AGG 0.639328 contig168end:-2681 MsG0080048006.01.T01:intergenic
GTTAGCGGCGGAGTCGTAGG+TGG 0.640286 contig168end:-3457 MsG0080048006.01.T01:intergenic
CGCGCTTAAAGAATGCGAGA+GGG 0.646475 contig168end:+4235 MsG0080048006.01.T01:CDS
TTAGAGATAAAATGCCTGCA+AGG 0.646765 contig168end:+4098 MsG0080048006.01.T01:CDS
TACGGTGAATTAGGAACAGG+AGG 0.647425 contig168end:+3631 MsG0080048006.01.T01:CDS
GATTGGAAAAGTCGATGCAA+CGG 0.648239 contig168end:+4391 MsG0080048006.01.T01:CDS
TCAGGCTCAACTCGTCGCGG+TGG 0.649482 contig168end:+3300 MsG0080048006.01.T01:intron
ACGCGCTTAAAGAATGCGAG+AGG 0.653096 contig168end:+4234 MsG0080048006.01.T01:CDS
TAGTGTGTTGAGTCTCACAA+TGG 0.663621 contig168end:-2626 MsG0080048006.01.T01:intergenic
TAATTTGTGATTGTTAGCGG+CGG 0.667633 contig168end:-3469 MsG0080048006.01.T01:intergenic
TTCTATATTAGGTCCCAACG+TGG 0.671040 contig168end:+4322 MsG0080048006.01.T01:CDS
AGTCGTAGGTGGTGAAACCG+AGG 0.675434 contig168end:-3446 MsG0080048006.01.T01:intergenic
AGCTCAGGCTCAACTCGTCG+CGG 0.679160 contig168end:+3297 MsG0080048006.01.T01:intron
AAAAGCACATAGGACATTGG+GGG 0.690062 contig168end:-2585 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTGTAAGAAATATTTA+AGG - contig168end:2823-2842 MsG0080048006.01.T01:intergenic 10.0%
!! AAACAAAAATTCTCAAACTA+TGG + contig168end:3192-3211 MsG0080048006.01.T01:intron 20.0%
!! GACAATAATAGAGTAAATAA+GGG - contig168end:4462-4481 MsG0080048006.01.T01:intergenic 20.0%
!! TGACAATAATAGAGTAAATA+AGG - contig168end:4463-4482 MsG0080048006.01.T01:intergenic 20.0%
!!! TTTAAGGTATACAACAAAAA+CGG + contig168end:3947-3966 MsG0080048006.01.T01:intron 20.0%
! AAAACAACTGAAAATGTGAA+CGG + contig168end:4350-4369 MsG0080048006.01.T01:CDS 25.0%
! AATTATTCATTCTCAAGCTA+CGG + contig168end:3487-3506 MsG0080048006.01.T01:CDS 25.0%
! ATTTAAGGACAATTACATGT+AGG - contig168end:2808-2827 MsG0080048006.01.T01:intergenic 25.0%
! CTTTAGATAGAAGAAATTGA+GGG - contig168end:2989-3008 MsG0080048006.01.T01:intergenic 25.0%
! TAATTTCGATGATAGAGATA+AGG - contig168end:4132-4151 MsG0080048006.01.T01:intergenic 25.0%
!! ATTTTGTGGTTTTTCTTACA+TGG - contig168end:2712-2731 MsG0080048006.01.T01:intergenic 25.0%
!! GAATAATTTGTGATTGTTAG+CGG - contig168end:3475-3494 MsG0080048006.01.T01:intergenic 25.0%
!! TGATATAAGAAAAAGCACAT+AGG - contig168end:2598-2617 MsG0080048006.01.T01:intergenic 25.0%
!! TTTGGAATAGGAATATTCTT+GGG - contig168end:2890-2909 MsG0080048006.01.T01:intergenic 25.0%
!! TTTTGTATATGAATGAATGC+AGG + contig168end:2840-2859 MsG0080048006.01.T01:intron 25.0%
!!! AGAATTTTTGTTTGAGTAGA+CGG - contig168end:3185-3204 MsG0080048006.01.T01:intergenic 25.0%
!!! ATAAGTTTTGAATCCAAACA+CGG - contig168end:3912-3931 MsG0080048006.01.T01:intergenic 25.0%
!!! TTTTGTTTGAAGAGTTTATG+AGG - contig168end:3043-3062 MsG0080048006.01.T01:intergenic 25.0%
AAAAGCAGTTTCAAAGCTTA+CGG + contig168end:3699-3718 MsG0080048006.01.T01:CDS 30.0%
AAAATTCTCAAACTATGGCT+CGG + contig168end:3197-3216 MsG0080048006.01.T01:intron 30.0%
AAACAACTGAAAATGTGAAC+GGG + contig168end:4351-4370 MsG0080048006.01.T01:CDS 30.0%
AACACAAACTTCACAAACTA+CGG + contig168end:3360-3379 MsG0080048006.01.T01:CDS 30.0%
AATGACACTTTCACTGAATA+TGG + contig168end:3866-3885 MsG0080048006.01.T01:CDS 30.0%
ACTTTCACTGAATATGGAAA+AGG + contig168end:3872-3891 MsG0080048006.01.T01:CDS 30.0%
ATAACCACTTAGTTAAGGAA+AGG - contig168end:2782-2801 MsG0080048006.01.T01:intergenic 30.0%
ATTATGGAGAATTGATGAGA+AGG - contig168end:3068-3087 MsG0080048006.01.T01:intergenic 30.0%
ATTCTTCAATCAACATTCAC+CGG + contig168end:3607-3626 MsG0080048006.01.T01:CDS 30.0%
CAAGTATGAAAATGAGAGTA+TGG - contig168end:2735-2754 MsG0080048006.01.T01:intergenic 30.0%
GATAATCAAATTGCATTGCA+TGG + contig168end:2754-2773 MsG0080048006.01.T01:intron 30.0%
GATGATTCGTTTCAAAGTTA+CGG + contig168end:3783-3802 MsG0080048006.01.T01:intron 30.0%
GGATTCAAAACTTATGGATT+GGG + contig168end:3917-3936 MsG0080048006.01.T01:CDS 30.0%
GTAAATAGATTTGTAGAACC+AGG + contig168end:4019-4038 MsG0080048006.01.T01:intron 30.0%
GTGTTTGGATTCAAAACTTA+TGG + contig168end:3911-3930 MsG0080048006.01.T01:CDS 30.0%
TCATTCAAAAACTACTCCAA+CGG + contig168end:3240-3259 MsG0080048006.01.T01:intron 30.0%
TGGATTCAAAACTTATGGAT+TGG + contig168end:3916-3935 MsG0080048006.01.T01:CDS 30.0%
TTATGGAGAATTGATGAGAA+GGG - contig168end:3067-3086 MsG0080048006.01.T01:intergenic 30.0%
! GAATGTTGATTGAAGAATGT+TGG - contig168end:3605-3624 MsG0080048006.01.T01:intergenic 30.0%
! GACTTTTCCGTTTCTATATT+AGG + contig168end:4311-4330 MsG0080048006.01.T01:CDS 30.0%
! GCTTTAGATAGAAGAAATTG+AGG - contig168end:2990-3009 MsG0080048006.01.T01:intergenic 30.0%
! GGCATTTTATCTCTAATATC+TGG - contig168end:4094-4113 MsG0080048006.01.T01:intergenic 30.0%
! GTTTGGAATAGGAATATTCT+TGG - contig168end:2891-2910 MsG0080048006.01.T01:intergenic 30.0%
!! AAATGAGAGTATGGATTTTG+TGG - contig168end:2726-2745 MsG0080048006.01.T01:intergenic 30.0%
!! GTTTGTCTTTTGTTTGGAAT+AGG - contig168end:2902-2921 MsG0080048006.01.T01:intergenic 30.0%
!!! ATTTGTTGGTTTTTGTGGAT+TGG - contig168end:3147-3166 MsG0080048006.01.T01:intergenic 30.0%
AAAATGTGAACGGGTCAAAA+AGG + contig168end:4360-4379 MsG0080048006.01.T01:CDS 35.0%
AACAATTTCAGCACAATCAG+TGG + contig168end:3989-4008 MsG0080048006.01.T01:intron 35.0%
AGAGAGTCGATGTTAAAAGA+AGG + contig168end:4052-4071 MsG0080048006.01.T01:intron 35.0%
CCGGTAACTTACAAAAGAAT+CGG - contig168end:3745-3764 MsG0080048006.01.T01:intergenic 35.0%
GATAGAAGAAATTGAGGGAT+TGG - contig168end:2984-3003 MsG0080048006.01.T01:intergenic 35.0%
GGACAATAACCACTTAGTTA+AGG - contig168end:2787-2806 MsG0080048006.01.T01:intergenic 35.0%
GTTGGGACCTAATATAGAAA+CGG - contig168end:4321-4340 MsG0080048006.01.T01:intergenic 35.0%
GTTGTTTGTGTTTAAACCGT+TGG - contig168end:3259-3278 MsG0080048006.01.T01:intergenic 35.0%
TACGGTAACGATACAAATTC+TGG + contig168end:3505-3524 MsG0080048006.01.T01:intron 35.0%
TATGGACAATCGTTTAACGA+AGG + contig168end:3843-3862 MsG0080048006.01.T01:intron 35.0%
TCGTTCAAAAAATACAGCTC+AGG + contig168end:3282-3301 MsG0080048006.01.T01:intron 35.0%
TCTCAATCATTCAACAGCTA+CGG + contig168end:3529-3548 MsG0080048006.01.T01:intron 35.0%
TGAATATGGAAAAGGAGCTA+CGG + contig168end:3880-3899 MsG0080048006.01.T01:CDS 35.0%
TTAGAGATAAAATGCCTGCA+AGG + contig168end:4098-4117 MsG0080048006.01.T01:CDS 35.0%
TTCAAAGCGTACAGTTTCAA+CGG + contig168end:3667-3686 MsG0080048006.01.T01:CDS 35.0%
! AAAAAGCACATAGGACATTG+GGG - contig168end:2589-2608 MsG0080048006.01.T01:intergenic 35.0%
! AATTACCATTTTCATCCTCG+AGG + contig168end:4147-4166 MsG0080048006.01.T01:CDS 35.0%
! AGAAAAAGCACATAGGACAT+TGG - contig168end:2591-2610 MsG0080048006.01.T01:intergenic 35.0%
! ATATTCTTGGGTTTTGCCAA+TGG - contig168end:2878-2897 MsG0080048006.01.T01:intergenic 35.0%
! CCGATTCTTTTGTAAGTTAC+CGG + contig168end:3742-3761 MsG0080048006.01.T01:intron 35.0%
! GAAAAAGCACATAGGACATT+GGG - contig168end:2590-2609 MsG0080048006.01.T01:intergenic 35.0%
! GAAAGGGTTTGTCTTTTGTT+TGG - contig168end:2908-2927 MsG0080048006.01.T01:intergenic 35.0%
! GAAGTTTGAGTCTGATTTGT+TGG - contig168end:3161-3180 MsG0080048006.01.T01:intergenic 35.0%
! GACTCTCTGAAAAATTTACC+TGG - contig168end:4040-4059 MsG0080048006.01.T01:intergenic 35.0%
! TAATTTGTGATTGTTAGCGG+CGG - contig168end:3472-3491 MsG0080048006.01.T01:intergenic 35.0%
! TGGAGATTTTTGTGTTCCAA+TGG - contig168end:2953-2972 MsG0080048006.01.T01:intergenic 35.0%
!! TGGATTGGTAAAGTGTGTTT+GGG - contig168end:3132-3151 MsG0080048006.01.T01:intergenic 35.0%
!!! GTAAGCTTTGAAACTGCTTT+TGG - contig168end:3700-3719 MsG0080048006.01.T01:intergenic 35.0%
!!! GTCTGATTTGTTGGTTTTTG+TGG - contig168end:3152-3171 MsG0080048006.01.T01:intergenic 35.0%
AAAAAGAGAGCGACAAGAAC+AGG - contig168end:2688-2707 MsG0080048005.01.T01:intergenic 40.0%
AACTTCACAAACTACGGTTC+CGG + contig168end:3366-3385 MsG0080048006.01.T01:CDS 40.0%
ACTTACAAAAGAATCGGAGC+CGG - contig168end:3739-3758 MsG0080048006.01.T01:intergenic 40.0%
ATAAGGTAAAACGACCTTGC+AGG - contig168end:4115-4134 MsG0080048006.01.T01:intergenic 40.0%
ATTGGTAAAGTGTGTTTGGG+TGG - contig168end:3129-3148 MsG0080048006.01.T01:intergenic 40.0%
CAGGTTTCTATAGCAACCAT+TGG + contig168end:2859-2878 MsG0080048006.01.T01:intron 40.0%
CTAGTGTGGGAGAAACAAAA+CGG + contig168end:4261-4280 MsG0080048006.01.T01:CDS 40.0%
GAATATGGAAAAGGAGCTAC+GGG + contig168end:3881-3900 MsG0080048006.01.T01:CDS 40.0%
GATTGGAAAAGTCGATGCAA+CGG + contig168end:4391-4410 MsG0080048006.01.T01:CDS 40.0%
GGTTCCTTTCCTTAACTAAG+TGG + contig168end:2775-2794 MsG0080048006.01.T01:intron 40.0%
TAGTGTGTTGAGTCTCACAA+TGG - contig168end:2629-2648 MsG0080048006.01.T01:intergenic 40.0%
TCAAAAAGGACGGTGATGAT+TGG + contig168end:4374-4393 MsG0080048006.01.T01:CDS 40.0%
TCGAAGGAGCAGAATAAGTA+TGG - contig168end:3106-3125 MsG0080048006.01.T01:intergenic 40.0%
TGTTCCTAATTCACCGTAAC+CGG - contig168end:3629-3648 MsG0080048006.01.T01:intergenic 40.0%
TTCAACAGCTACGCAGAAAA+CGG + contig168end:3327-3346 MsG0080048006.01.T01:CDS 40.0%
TTCTATATTAGGTCCCAACG+TGG + contig168end:4322-4341 MsG0080048006.01.T01:CDS 40.0%
TTGAGGGATTGGAAGTTTGT+TGG - contig168end:2973-2992 MsG0080048006.01.T01:intergenic 40.0%
! AAAAGCACATAGGACATTGG+GGG - contig168end:2588-2607 MsG0080048006.01.T01:intergenic 40.0%
! CAATCAACATTCACCGGTTA+CGG + contig168end:3613-3632 MsG0080048006.01.T01:CDS 40.0%
! TTTTATCTCACTCAACCTCG+AGG - contig168end:4165-4184 MsG0080048006.01.T01:intergenic 40.0%
!! AGTTGTTTTCACAACCACGT+TGG - contig168end:4339-4358 MsG0080048006.01.T01:intergenic 40.0%
!! GACCATAAATTTGTTGTCCC+CGG + contig168end:2562-2581 MsG0080048006.01.T01:CDS 40.0%
!! GTGGATTGGTAAAGTGTGTT+TGG - contig168end:3133-3152 MsG0080048006.01.T01:intergenic 40.0%
!! GTTGTTTTCACAACCACGTT+GGG - contig168end:4338-4357 MsG0080048006.01.T01:intergenic 40.0%
!!! ATCGTAGCTTTTGAAGTCTC+CGG - contig168end:3406-3425 MsG0080048006.01.T01:intergenic 40.0%
AAAAGAATCGGAGCCGGAAA+CGG - contig168end:3733-3752 MsG0080048005.01.T01:intergenic 45.0%
AATCATCACCAACGTTAGCG+CGG - contig168end:3770-3789 MsG0080048006.01.T01:intergenic 45.0%
AGTTTCAAAGCTTACGGCAC+TGG + contig168end:3705-3724 MsG0080048006.01.T01:CDS 45.0%
ATAAGTATGGTGTGGAGCAG+AGG - contig168end:3093-3112 MsG0080048006.01.T01:intergenic 45.0%
ATGAGGTTTGCGTAGTGTTG+TGG - contig168end:3026-3045 MsG0080048006.01.T01:intergenic 45.0%
CTCAACCTCGAGGATGAAAA+TGG - contig168end:4155-4174 MsG0080048006.01.T01:intergenic 45.0%
CTCGTTAATGTACCCAACCT+CGG + contig168end:3429-3448 MsG0080048006.01.T01:CDS 45.0%
GCTGCGTCGTTTACGAATTA+TGG + contig168end:3825-3844 MsG0080048006.01.T01:intron 45.0%
GGAGCAGAATAAGTATGGTG+TGG - contig168end:3101-3120 MsG0080048006.01.T01:intergenic 45.0%
GGTTACGGTGAATTAGGAAC+AGG + contig168end:3628-3647 MsG0080048006.01.T01:CDS 45.0%
TAAGTATGGTGTGGAGCAGA+GGG - contig168end:3092-3111 MsG0080048006.01.T01:intergenic 45.0%
TACGGAAAACGTGTTCGTAG+CGG + contig168end:3547-3566 MsG0080048006.01.T01:intron 45.0%
TACGGTGAATTAGGAACAGG+AGG + contig168end:3631-3650 MsG0080048006.01.T01:CDS 45.0%
TACGGTGCAAAATCGAAAGC+CGG + contig168end:3801-3820 MsG0080048006.01.T01:intron 45.0%
TACGGTTCCGGTTCAAATTC+CGG + contig168end:3378-3397 MsG0080048006.01.T01:CDS 45.0%
TGTGAACGGGTCAAAAAGGA+CGG + contig168end:4364-4383 MsG0080048006.01.T01:CDS 45.0%
! TTCACCGGTTACGGTGAATT+AGG + contig168end:3622-3641 MsG0080048006.01.T01:CDS 45.0%
!! AAAAAGAAGCGTTTCACGCG+CGG + contig168end:4181-4200 MsG0080048006.01.T01:CDS 45.0%
!! AGTGAGGCTTTAGGAGTGAA+AGG - contig168end:2925-2944 MsG0080048006.01.T01:intergenic 45.0%
!! GTGAGGCTTTAGGAGTGAAA+GGG - contig168end:2924-2943 MsG0080048006.01.T01:intergenic 45.0%
!!! CGTTTTGTTTCTCCCACACT+AGG - contig168end:4263-4282 MsG0080048006.01.T01:intergenic 45.0%
AAAGCCTCACTCATCGCCAT+TGG + contig168end:2934-2953 MsG0080048006.01.T01:intron 50.0%
AAGTGTGTTTGGGTGGTCGA+AGG - contig168end:3122-3141 MsG0080048006.01.T01:intergenic 50.0%
ACGCGCTTAAAGAATGCGAG+AGG + contig168end:4234-4253 MsG0080048006.01.T01:CDS 50.0%
AGAGAGCGACAAGAACAGGA+AGG - contig168end:2684-2703 MsG0080048006.01.T01:intergenic 50.0%
ATGGCGATGAGTGAGGCTTT+AGG - contig168end:2934-2953 MsG0080048006.01.T01:intergenic 50.0%
ATTCGTAAACGACGCAGCAC+CGG - contig168end:3823-3842 MsG0080048006.01.T01:intergenic 50.0%
CGCGCTTAAAGAATGCGAGA+GGG + contig168end:4235-4254 MsG0080048006.01.T01:CDS 50.0%
GCGTAGTGTTGTGGTGTAAG+AGG - contig168end:3017-3036 MsG0080048006.01.T01:intergenic 50.0%
GCTACGGGTAAAACCGTGTT+TGG + contig168end:3896-3915 MsG0080048006.01.T01:CDS 50.0%
GGAAAAGTCGATGCAACGGA+CGG + contig168end:4395-4414 MsG0080048006.01.T01:CDS 50.0%
GGAGAAACAAAACGGTGCGT+TGG + contig168end:4269-4288 MsG0080048006.01.T01:CDS 50.0%
GGCCGGGGACAACAAATTTA+TGG - contig168end:2567-2586 MsG0080048006.01.T01:intergenic 50.0%
TAGGTGGTGAAACCGAGGTT+GGG - contig168end:3444-3463 MsG0080048006.01.T01:intergenic 50.0%
TGTTCCAATGGCGATGAGTG+AGG - contig168end:2941-2960 MsG0080048006.01.T01:intergenic 50.0%
!! AAAAGAAGCGTTTCACGCGC+GGG + contig168end:4182-4201 MsG0080048006.01.T01:CDS 50.0%
!! GTGTGGAGCAGAGGGTATTA+TGG - contig168end:3084-3103 MsG0080048006.01.T01:intergenic 50.0%
AAAGTCGATGCAACGGACGG+TGG + contig168end:4398-4417 MsG0080048006.01.T01:CDS 55.0%
AGTCGTAGGTGGTGAAACCG+AGG - contig168end:3449-3468 MsG0080048006.01.T01:intergenic 55.0%
ATGCGAGAGGGAACCTAGTG+TGG + contig168end:4247-4266 MsG0080048006.01.T01:CDS 55.0%
ATTGTTAGCGGCGGAGTCGT+AGG - contig168end:3463-3482 MsG0080048006.01.T01:intergenic 55.0%
GTAGGTGGTGAAACCGAGGT+TGG - contig168end:3445-3464 MsG0080048006.01.T01:intergenic 55.0%
TCCGGTTCAAATTCCGGCAC+CGG + contig168end:3384-3403 MsG0080048006.01.T01:CDS 55.0%
TGCGAGAGGGAACCTAGTGT+GGG + contig168end:4248-4267 MsG0080048006.01.T01:CDS 55.0%
! AACTATGGCTCGGCTCGACT+CGG + contig168end:3207-3226 MsG0080048006.01.T01:intron 55.0%
! TCCGGTGCCGGAATTTGAAC+CGG - contig168end:3388-3407 MsG0080048006.01.T01:intergenic 55.0%
! TGGATTGGGACGCGCGTTTA+AGG + contig168end:3931-3950 MsG0080048006.01.T01:CDS 55.0%
!! TACGGCACTGGTTCCGTTTC+CGG + contig168end:3717-3736 MsG0080048006.01.T01:CDS 55.0%
!!! GCTTTTGAAGTCTCCGGTGC+CGG - contig168end:3400-3419 MsG0080048006.01.T01:intergenic 55.0%
ACATAGGACATTGGGGGCCG+GGG - contig168end:2582-2601 MsG0080048006.01.T01:intergenic 60.0%
ACGGTGCGTTGGATCAGCTG+AGG + contig168end:4280-4299 MsG0080048006.01.T01:CDS 60.0%
AGCTCAGGCTCAACTCGTCG+CGG + contig168end:3297-3316 MsG0080048006.01.T01:intron 60.0%
CACATAGGACATTGGGGGCC+GGG - contig168end:2583-2602 MsG0080048006.01.T01:intergenic 60.0%
CACCAACGTTAGCGCGGTTC+CGG - contig168end:3764-3783 MsG0080048006.01.T01:intergenic 60.0%
CGGTGCGTTGGATCAGCTGA+GGG + contig168end:4281-4300 MsG0080048006.01.T01:CDS 60.0%
GCACATAGGACATTGGGGGC+CGG - contig168end:2584-2603 MsG0080048006.01.T01:intergenic 60.0%
TACCGGAACCGCGCTAACGT+TGG + contig168end:3759-3778 MsG0080048006.01.T01:intron 60.0%
GTTAGCGGCGGAGTCGTAGG+TGG - contig168end:3460-3479 MsG0080048006.01.T01:intergenic 65.0%
TCAGGCTCAACTCGTCGCGG+TGG + contig168end:3300-3319 MsG0080048006.01.T01:intron 65.0%
! GAAGCGTTTCACGCGCGGGA+TGG + contig168end:4186-4205 MsG0080048006.01.T01:CDS 65.0%
!! TATGGCTCGGCTCGACTCGG+CGG + contig168end:3210-3229 MsG0080048006.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
contig168end gene 2553 4499 2553 ID=MsG0080048006.01;Name=MsG0080048006.01
contig168end mRNA 2553 4499 2553 ID=MsG0080048006.01.T01;Parent=MsG0080048006.01;Name=MsG0080048006.01.T01;_AED=0.31;_eAED=0.32;_QI=0|0|0|1|0.5|0.4|5|0|344
contig168end exon 2553 2712 2553 ID=MsG0080048006.01.T01:exon:24495;Parent=MsG0080048006.01.T01
contig168end exon 3304 3508 3304 ID=MsG0080048006.01.T01:exon:24494;Parent=MsG0080048006.01.T01
contig168end exon 3576 3752 3576 ID=MsG0080048006.01.T01:exon:24493;Parent=MsG0080048006.01.T01
contig168end exon 3865 3952 3865 ID=MsG0080048006.01.T01:exon:24492;Parent=MsG0080048006.01.T01
contig168end exon 4095 4499 4095 ID=MsG0080048006.01.T01:exon:24491;Parent=MsG0080048006.01.T01
contig168end CDS 2553 2712 2553 ID=MsG0080048006.01.T01:cds;Parent=MsG0080048006.01.T01
contig168end CDS 3304 3508 3304 ID=MsG0080048006.01.T01:cds;Parent=MsG0080048006.01.T01
contig168end CDS 3576 3752 3576 ID=MsG0080048006.01.T01:cds;Parent=MsG0080048006.01.T01
contig168end CDS 3865 3952 3865 ID=MsG0080048006.01.T01:cds;Parent=MsG0080048006.01.T01
contig168end CDS 4095 4499 4095 ID=MsG0080048006.01.T01:cds;Parent=MsG0080048006.01.T01
Gene Sequence

>MsG0080048006.01.T01

ATGTGGGGAGACCATAAATTTGTTGTCCCCGGCCCCCAATGTCCTATGTGCTTTTTCTTATATCACTCTCACTCCATTGTGAGACTCAACACACTAAACTCAACATCTCAAAATGAAAATTTACTTCACCTTCCTGTTCTTGTCGCTCTCTTTTTTCCATGCTCAACTCGTCGCGGTGGAGAATTCAACAGCTACGCAGAAAACGGCAATGTAGCTAACACAAACTTCACAAACTACGGTTCCGGTTCAAATTCCGGCACCGGAGACTTCAAAAGCTACGATAAACTCGTTAATGTACCCAACCTCGGTTTCACCACCTACGACTCCGCCGCTAACAATCACAAATTATTCATTCTCAAGCTACGCGATTTCATCAATTATGCAGTTAGTTCCAACATTCTTCAATCAACATTCACCGGTTACGGTGAATTAGGAACAGGAGGTGCAAACGATTCTTTCAAAGCGTACAGTTTCAACGGTAATAATCCAAAAGCAGTTTCAAAGCTTACGGCACTGGTTCCGTTTCCGGCTCCGATTCTTTTGAATGACACTTTCACTGAATATGGAAAAGGAGCTACGGGTAAAACCGTGTTTGGATTCAAAACTTATGGATTGGGACGCGCGTTTAAGATATTAGAGATAAAATGCCTGCAAGGTCGTTTTACCTTATCTCTATCATCGAAATTACCATTTTCATCCTCGAGGTTGAGTGAGATAAAAAGAAGCGTTTCACGCGCGGGATGGTCAGCGACAGAGCGCGTGATAGTTAACGCGCTTAAAGAATGCGAGAGGGAACCTAGTGTGGGAGAAACAAAACGGTGCGTTGGATCAGCTGAGGGTATGATTGACTTTTCCGTTTCTATATTAGGTCCCAACGTGGTTGTGAAAACAACTGAAAATGTGAACGGGTCAAAAAGGACGGTGATGATTGGAAAAGTCGATGCAACGGACGGTGGAAAAGTGATTAAATCAGTTGTCATCATCAGAGTTTGTACCCTTATTTACTCTATTATTGTCACTCTGTCCCTAAAGTGA
Protein sequence

>MsG0080048006.01.T01

MWGDHKFVVPGPQCPMCFFLYHSHSIVRLNTLNSTSQNENLLHLPVLVALFFPCSTRRGGEFNSYAENGNVANTNFTNYGSGSNSGTGDFKSYDKLVNVPNLGFTTYDSAANNHKLFILKLRDFINYAVSSNILQSTFTGYGELGTGGANDSFKAYSFNGNNPKAVSKLTALVPFPAPILLNDTFTEYGKGATGKTVFGFKTYGLGRAFKILEIKCLQGRFTLSLSSKLPFSSSRLSEIKRSVSRAGWSATERVIVNALKECEREPSVGETKRCVGSAEGMIDFSVSILGPNVVVKTTENVNGSKRTVMIGKVDATDGGKVIKSVVIIRVCTLIYSIIVTLSLK*