AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048211.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048211.01.T01 MTR_1g087370 77.714 175 0 1 1 175 1 136 2.17e-92 265
MsG0080048211.01.T01 MTR_1g087370 90.476 105 7 1 1 102 1 105 7.82e-65 194
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048211.01.T01 AT1G02680 51.007 149 30 2 15 163 19 124 8.97e-45 144

Find 46 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAAAGAGTTAAGCGATTTA+AGG 0.160135 contig244end:-28506 None:intergenic
TGAACCAATACAGGTTATTT+AGG 0.184660 contig244end:-28429 None:intergenic
TCCATAAGCGCCACACTTTC+AGG 0.195120 contig244end:-27585 None:intergenic
CCTTAAATCGCTTAACTCTT+TGG 0.215778 contig244end:+28506 MsG0080048211.01.T01:CDS
TCCTGAAAGTGTGGCGCTTA+TGG 0.280852 contig244end:+27584 MsG0080048211.01.T01:CDS
ACCTGTATTGGTTCAATAAT+CGG 0.281444 contig244end:+28437 MsG0080048211.01.T01:CDS
AATACAGGTTATTTAGGATT+AGG 0.310456 contig244end:-28423 None:intergenic
GCTTAACTCTTTGGATCAAC+AGG 0.345975 contig244end:+28515 MsG0080048211.01.T01:CDS
GTACATAAAGCACAAGATAT+TGG 0.348377 contig244end:+27741 MsG0080048211.01.T01:CDS
TAATCCTAAATAACCTGTAT+TGG 0.366049 contig244end:+28425 MsG0080048211.01.T01:CDS
TCTGTACAACGGTTAAGTTT+TGG 0.401058 contig244end:-29323 None:intergenic
GAAAACTCCTCTTCTGCGCT+TGG 0.409109 contig244end:-27337 None:intergenic
ACCGATTATTGAACCAATAC+AGG 0.430433 contig244end:-28438 None:intergenic
GCAGAATTGCTCATTGTTGT+TGG 0.451929 contig244end:-27258 None:intergenic
TCAACCATTCATCCTTCTGC+TGG 0.456641 contig244end:-29437 None:intergenic
TATCTGTCAAATCTGAGGTT+AGG 0.463981 contig244end:+28470 MsG0080048211.01.T01:CDS
TGTAGAATATATTACAGAAC+TGG 0.468418 contig244end:+27617 MsG0080048211.01.T01:CDS
CTGCGCTTGGATGAAGTGTC+TGG 0.471299 contig244end:-27324 None:intergenic
ATAGTGCAGCACATGATGTA+TGG 0.483674 contig244end:+27446 MsG0080048211.01.T01:intron
ATCTTCTTACCTCGAAGAAA+AGG 0.491018 contig244end:-26508 None:intergenic
CAAACATGCTAGTAAACTGC+TGG 0.494351 contig244end:-29596 None:intergenic
CAGCACATGATGTATGGCTT+CGG 0.502212 contig244end:+27452 MsG0080048211.01.T01:CDS
ACTTCATCCAAGCGCAGAAG+AGG 0.506271 contig244end:+27330 MsG0080048211.01.T01:CDS
AGCGCCACACTTTCAGGAAG+TGG 0.521650 contig244end:-27579 None:intergenic
GTACCTTGCGAATCAAATAG+AGG 0.544458 contig244end:-27797 None:intergenic
ACAATGAGCAATTCTGCTGC+CGG 0.562228 contig244end:+27264 MsG0080048211.01.T01:exon
TGAACGTGGTAGAAAGAAAG+AGG 0.592008 contig244end:+26468 MsG0080048211.01.T01:five_prime_UTR
ACAAGATATTGGATCACAGA+GGG 0.592434 contig244end:+27752 MsG0080048211.01.T01:CDS
TGAACCAGCAGAAGGATGAA+TGG 0.594825 contig244end:+29433 MsG0080048211.01.T01:exon
GAGGTGGATGAACCAGCAGA+AGG 0.597040 contig244end:+29425 MsG0080048211.01.T01:CDS
TTTCCTCTATTTGATTCGCA+AGG 0.600469 contig244end:+27794 MsG0080048211.01.T01:CDS
AACATGATGAAGAGAATCTG+AGG 0.603292 contig244end:+29393 MsG0080048211.01.T01:CDS
AACTGCTGGAACGAGTTATG+TGG 0.604175 contig244end:-29582 None:intergenic
TAATGTATCTGTCAAATCTG+AGG 0.611831 contig244end:+28465 MsG0080048211.01.T01:CDS
TGATGAAGAGAATCTGAGGA+AGG 0.619169 contig244end:+29397 MsG0080048211.01.T01:CDS
CTTAACTCTTTGGATCAACA+GGG 0.621688 contig244end:+28516 MsG0080048211.01.T01:CDS
CGCTTGGATGAAGTGTCTGG+AGG 0.624183 contig244end:-27321 None:intergenic
CACAAGATATTGGATCACAG+AGG 0.626166 contig244end:+27751 MsG0080048211.01.T01:CDS
TGAAGTGTCTGGAGGTTGTG+AGG 0.629236 contig244end:-27313 None:intergenic
ATGAAGAGCTAAAACAAGCA+AGG 0.630743 contig244end:+29360 MsG0080048211.01.T01:CDS
TTAGCCACTTCCTGAAAGTG+TGG 0.632131 contig244end:+27575 MsG0080048211.01.T01:intron
TAGACAACAGTTCTGTACAA+CGG 0.672921 contig244end:-29334 None:intergenic
ACACATCATCTACGTGAACG+TGG 0.673440 contig244end:+26454 MsG0080048211.01.T01:five_prime_UTR
AAGATATTGGATCACAGAGG+GGG 0.677224 contig244end:+27754 MsG0080048211.01.T01:CDS
CAAGATATTGGATCACAGAG+GGG 0.705641 contig244end:+27753 MsG0080048211.01.T01:CDS
GAAATACATGATAGTCAACA+AGG 0.706007 contig244end:-29513 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTTATTTTTTTGTCA+AGG + contig244end:27719-27738 MsG0080048211.01.T01:intron 10.0%
!! AAAAATTATTACATTCTCTT+TGG - contig244end:27362-27381 None:intergenic 15.0%
!! TATTCATTAACAATGAATAT+GGG + contig244end:28555-28574 MsG0080048211.01.T01:intron 15.0%
!! TTATTCATTAACAATGAATA+TGG + contig244end:28554-28573 MsG0080048211.01.T01:intron 15.0%
!! AAACAAGAAAACAAACAAAA+GGG - contig244end:26801-26820 None:intergenic 20.0%
!! AAAGTTGCAAATATAAATCT+TGG + contig244end:27833-27852 MsG0080048211.01.T01:intron 20.0%
!! AAGTTGCAAATATAAATCTT+GGG + contig244end:27834-27853 MsG0080048211.01.T01:intron 20.0%
!! AATAAATAAAAAATCGCGTA+AGG - contig244end:27710-27729 None:intergenic 20.0%
!! ACGCTAAAATTTAAAAACTT+AGG - contig244end:27388-27407 None:intergenic 20.0%
!! TAAACAAGAAAACAAACAAA+AGG - contig244end:26802-26821 None:intergenic 20.0%
!! TAATCCTATAGAATAAAAAC+TGG - contig244end:27118-27137 None:intergenic 20.0%
!! TACAATTCTGTAAAACTAAT+GGG + contig244end:26760-26779 MsG0080048211.01.T01:intron 20.0%
!! TATATAATCTTAGCATCTTA+TGG - contig244end:28892-28911 None:intergenic 20.0%
!! TATGATAAAATTTCCAATTC+AGG - contig244end:26874-26893 None:intergenic 20.0%
!! TATTAGAAAAATGCACTAAA+AGG + contig244end:29089-29108 MsG0080048211.01.T01:intron 20.0%
!! TTACAATTCTGTAAAACTAA+TGG + contig244end:26759-26778 MsG0080048211.01.T01:intron 20.0%
!!! ATTTCTCTTTTAATACATGT+TGG - contig244end:27972-27991 None:intergenic 20.0%
!!! ATTTTAGTAACTATAGTTAG+AGG - contig244end:27221-27240 None:intergenic 20.0%
!!! ATTTTTATACGAGTCTTTAA+AGG + contig244end:28301-28320 MsG0080048211.01.T01:intron 20.0%
!!! GAAAAATAACCTTCATTTTA+GGG - contig244end:29041-29060 None:intergenic 20.0%
!!! TGAAAAATAACCTTCATTTT+AGG - contig244end:29042-29061 None:intergenic 20.0%
!!! TTTCTCTTTTAATACATGTT+GGG - contig244end:27971-27990 None:intergenic 20.0%
!!! TTTTTGTATTGAGTCATTAT+GGG - contig244end:26659-26678 None:intergenic 20.0%
! AAAATATCAAGATTTGATCG+TGG - contig244end:29263-29282 None:intergenic 25.0%
! AACAAGAAAACAAACAAAAG+GGG - contig244end:26800-26819 None:intergenic 25.0%
! AACTTATCTACTCCTATTAA+AGG + contig244end:29191-29210 MsG0080048211.01.T01:intron 25.0%
! AATAAGGTTAATACCTGAAT+TGG + contig244end:26858-26877 MsG0080048211.01.T01:intron 25.0%
! AATGACTCAATACAAAAACA+GGG + contig244end:26661-26680 MsG0080048211.01.T01:intron 25.0%
! AATTTATCTTCAAATACTGC+TGG + contig244end:27870-27889 MsG0080048211.01.T01:intron 25.0%
! ACAATTCTGTAAAACTAATG+GGG + contig244end:26761-26780 MsG0080048211.01.T01:intron 25.0%
! ACTTATCTACTCCTATTAAA+GGG + contig244end:29192-29211 MsG0080048211.01.T01:intron 25.0%
! AGAACAATAATTGAAGATGA+TGG + contig244end:26716-26735 MsG0080048211.01.T01:intron 25.0%
! AGTTGCAAATATAAATCTTG+GGG + contig244end:27835-27854 MsG0080048211.01.T01:intron 25.0%
! CTTATCATTGATCAAACTTT+GGG + contig244end:28812-28831 MsG0080048211.01.T01:intron 25.0%
! GACTCGTATAAAAATTGAAT+TGG - contig244end:28296-28315 None:intergenic 25.0%
! GATAGAATGAGAATTACATT+AGG - contig244end:27485-27504 None:intergenic 25.0%
! GGTAATAAGTTCTCAAATTA+GGG + contig244end:26682-26701 MsG0080048211.01.T01:intron 25.0%
! TAATCCTAAATAACCTGTAT+TGG + contig244end:28425-28444 MsG0080048211.01.T01:CDS 25.0%
! TAATGACTCAATACAAAAAC+AGG + contig244end:26660-26679 MsG0080048211.01.T01:intron 25.0%
! TATAGAAACCAATGAAGATT+AGG - contig244end:27429-27448 None:intergenic 25.0%
! TATAGTTACTAAAATGACAG+TGG + contig244end:27226-27245 MsG0080048211.01.T01:intron 25.0%
! TCTTATCATTGATCAAACTT+TGG + contig244end:28811-28830 MsG0080048211.01.T01:intron 25.0%
! TGAAGACCATATATTCATAA+AGG - contig244end:28168-28187 None:intergenic 25.0%
! TGTAGAATATATTACAGAAC+TGG + contig244end:27617-27636 MsG0080048211.01.T01:CDS 25.0%
!! AATACAGGTTATTTAGGATT+AGG - contig244end:28426-28445 None:intergenic 25.0%
!! CATTCCAGTTTTTATTCTAT+AGG + contig244end:27111-27130 MsG0080048211.01.T01:intron 25.0%
!! GTGGTTTAAAGTTAATAGTT+GGG + contig244end:28237-28256 MsG0080048211.01.T01:intron 25.0%
!! GTTTTTGTATTGAGTCATTA+TGG - contig244end:26660-26679 None:intergenic 25.0%
AATGAAAGAAAAAGCAGTGT+GGG - contig244end:26538-26557 None:intergenic 30.0%
AGAAAAATGCACTAAAAGGA+AGG + contig244end:29093-29112 MsG0080048211.01.T01:intron 30.0%
AGAACCATTAATACCAACTT+TGG - contig244end:28378-28397 None:intergenic 30.0%
AGTTGTTAGTTGTTAGTTAC+TGG + contig244end:28261-28280 MsG0080048211.01.T01:intron 30.0%
ATCTTAAACTTGATTGACCA+TGG + contig244end:28649-28668 MsG0080048211.01.T01:intron 30.0%
ATTGTCTTCCTAATCTTCAT+TGG + contig244end:27418-27437 MsG0080048211.01.T01:intron 30.0%
CTTCAACAATGAACACAATA+GGG - contig244end:26622-26641 None:intergenic 30.0%
GAAATACATGATAGTCAACA+AGG - contig244end:29516-29535 None:intergenic 30.0%
GGGTAATAAGTTCTCAAATT+AGG + contig244end:26681-26700 MsG0080048211.01.T01:intron 30.0%
GGTCTTCAATTGCTTATTAT+AGG + contig244end:28180-28199 MsG0080048211.01.T01:intron 30.0%
GTACATAAAGCACAAGATAT+TGG + contig244end:27741-27760 MsG0080048211.01.T01:CDS 30.0%
TAACTATAGTTAGAGGAAAG+AGG - contig244end:27214-27233 None:intergenic 30.0%
TAATGTATCTGTCAAATCTG+AGG + contig244end:28465-28484 MsG0080048211.01.T01:CDS 30.0%
TGAACCAATACAGGTTATTT+AGG - contig244end:28432-28451 None:intergenic 30.0%
TTAGCTAGAAATATCACTTC+TGG + contig244end:28138-28157 MsG0080048211.01.T01:intron 30.0%
TTTGTCAAAATGGCTCAATA+AGG + contig244end:26842-26861 MsG0080048211.01.T01:intron 30.0%
! GGTGGTTTAAAGTTAATAGT+TGG + contig244end:28236-28255 MsG0080048211.01.T01:intron 30.0%
! GTTTAACATTTTTGTTCGCA+AGG + contig244end:26817-26836 MsG0080048211.01.T01:intron 30.0%
!! ACCTGTATTGGTTCAATAAT+CGG + contig244end:28437-28456 MsG0080048211.01.T01:CDS 30.0%
!! TAAGCCAAAGTTGGTATTAA+TGG + contig244end:28371-28390 MsG0080048211.01.T01:intron 30.0%
!!! TTAGTTTTCTGGTTCCTTAA+AGG + contig244end:28934-28953 MsG0080048211.01.T01:intron 30.0%
AAAAAATCGCGTAAGGACAA+AGG - contig244end:27703-27722 None:intergenic 35.0%
AACATGATGAAGAGAATCTG+AGG + contig244end:29393-29412 MsG0080048211.01.T01:CDS 35.0%
AAGCTTCTATAAGCCAAAGT+TGG + contig244end:28362-28381 MsG0080048211.01.T01:intron 35.0%
ACAAGATATTGGATCACAGA+GGG + contig244end:27752-27771 MsG0080048211.01.T01:CDS 35.0%
ACAGCATAATTATGCATGAG+TGG - contig244end:28689-28708 None:intergenic 35.0%
ACCATAAAGCTTATGGAAGA+AGG - contig244end:28759-28778 None:intergenic 35.0%
ACCGATTATTGAACCAATAC+AGG - contig244end:28441-28460 None:intergenic 35.0%
ACTGTTAGCACTATAATAGC+CGG + contig244end:28579-28598 MsG0080048211.01.T01:intron 35.0%
AGTTTATATCTCAGAACTCC+TGG - contig244end:28998-29017 None:intergenic 35.0%
ATCTTCTTACCTCGAAGAAA+AGG - contig244end:26511-26530 None:intergenic 35.0%
ATGAAGAGCTAAAACAAGCA+AGG + contig244end:29360-29379 MsG0080048211.01.T01:CDS 35.0%
CAGAGAATTCTTTCTCAAGT+AGG + contig244end:28331-28350 MsG0080048211.01.T01:intron 35.0%
CCAAAGAGTTAAGCGATTTA+AGG - contig244end:28509-28528 None:intergenic 35.0%
CCATAAAGCTTATGGAAGAA+GGG - contig244end:28758-28777 None:intergenic 35.0%
CCTTAAATCGCTTAACTCTT+TGG + contig244end:28506-28525 MsG0080048211.01.T01:CDS 35.0%
CTTAACTCTTTGGATCAACA+GGG + contig244end:28516-28535 MsG0080048211.01.T01:CDS 35.0%
GAATGAAAGAAAAAGCAGTG+TGG - contig244end:26539-26558 None:intergenic 35.0%
GCTTCAACAATGAACACAAT+AGG - contig244end:26623-26642 None:intergenic 35.0%
GGTCTGCCTTTATGAATATA+TGG + contig244end:28159-28178 MsG0080048211.01.T01:intron 35.0%
TAACTTTAAACCACCATTGC+AGG - contig244end:28231-28250 None:intergenic 35.0%
TAGACAACAGTTCTGTACAA+CGG - contig244end:29337-29356 None:intergenic 35.0%
TCCTAGAACCATAAAGCTTA+TGG - contig244end:28766-28785 None:intergenic 35.0%
TCTCAAATTAGGGCTTGATT+TGG + contig244end:26692-26711 MsG0080048211.01.T01:intron 35.0%
TCTTCAAATACTGCTGGAAA+TGG + contig244end:27876-27895 MsG0080048211.01.T01:intron 35.0%
TTAAAGGCTCATACACACTT+CGG + contig244end:28950-28969 MsG0080048211.01.T01:intron 35.0%
TTAACTTAGTCTAAGTGCCA+TGG + contig244end:27668-27687 MsG0080048211.01.T01:intron 35.0%
! AACTTTGGCTTATAGAAGCT+TGG - contig244end:28363-28382 None:intergenic 35.0%
! ACAAGAAGCTTTATGAGTCT+TGG - contig244end:29288-29307 None:intergenic 35.0%
! ACTTTGGCTTATAGAAGCTT+GGG - contig244end:28362-28381 None:intergenic 35.0%
! CAAGTTTAAGATGAGGAAGT+TGG - contig244end:28641-28660 None:intergenic 35.0%
! GGTCAATCAAGTTTAAGATG+AGG - contig244end:28648-28667 None:intergenic 35.0%
! TATCTGTCAAATCTGAGGTT+AGG + contig244end:28470-28489 MsG0080048211.01.T01:CDS 35.0%
! TATGGTGCATTTAGCTTCAT+TGG - contig244end:28874-28893 None:intergenic 35.0%
! TCCATAAGCTTTATGGTTCT+AGG + contig244end:28762-28781 MsG0080048211.01.T01:intron 35.0%
! TCTGTACAACGGTTAAGTTT+TGG - contig244end:29326-29345 None:intergenic 35.0%
! TCTTTTAATACATGTTGGGC+AGG - contig244end:27967-27986 None:intergenic 35.0%
! TTTCCTCTATTTGATTCGCA+AGG + contig244end:27794-27813 MsG0080048211.01.T01:CDS 35.0%
!! CAATTTTGCTGTGTAATGCT+AGG + contig244end:26585-26604 MsG0080048211.01.T01:intron 35.0%
!!! TCTTTGTTTTGATGAGGTTC+CGG - contig244end:27286-27305 None:intergenic 35.0%
!!! TTTTTTGATACAACGTGTGC+AGG - contig244end:27166-27185 None:intergenic 35.0%
AAAGAAAAAGCAGTGTGGGT+TGG - contig244end:26534-26553 None:intergenic 40.0%
AAAGCTTATGGAAGAAGGGA+GGG - contig244end:28754-28773 None:intergenic 40.0%
AAGATATTGGATCACAGAGG+GGG + contig244end:27754-27773 MsG0080048211.01.T01:CDS 40.0%
ATAAGCATGCTATGTCACAG+TGG + contig244end:28205-28224 MsG0080048211.01.T01:intron 40.0%
ATAGTGCAGCACATGATGTA+TGG + contig244end:27446-27465 MsG0080048211.01.T01:intron 40.0%
ATGCACATGCCCTAAAATGA+AGG + contig244end:29029-29048 MsG0080048211.01.T01:intron 40.0%
ATTATGCATGAGTGGTCCAA+AGG - contig244end:28681-28700 None:intergenic 40.0%
CAAACATGCTAGTAAACTGC+TGG - contig244end:29599-29618 None:intergenic 40.0%
CAAGATATTGGATCACAGAG+GGG + contig244end:27753-27772 MsG0080048211.01.T01:CDS 40.0%
CACAAGATATTGGATCACAG+AGG + contig244end:27751-27770 MsG0080048211.01.T01:CDS 40.0%
CATTCGTTCGATTCAGTTAC+TGG + contig244end:26554-26573 MsG0080048211.01.T01:intron 40.0%
CCCTTCTTCCATAAGCTTTA+TGG + contig244end:28755-28774 MsG0080048211.01.T01:intron 40.0%
CCTATTAAAGGGTCGAATTC+AGG + contig244end:29203-29222 MsG0080048211.01.T01:intron 40.0%
CCTGAATTCGACCCTTTAAT+AGG - contig244end:29206-29225 None:intergenic 40.0%
CTATAGGATTATGTGTGCAC+AGG + contig244end:27127-27146 MsG0080048211.01.T01:intron 40.0%
GAAGTGTGTATGAGCCTTTA+AGG - contig244end:28951-28970 None:intergenic 40.0%
GCAGAATTGCTCATTGTTGT+TGG - contig244end:27261-27280 None:intergenic 40.0%
GCTGTTGTCACTATTCTCAA+GGG + contig244end:27913-27932 MsG0080048211.01.T01:intron 40.0%
GCTTAAACCTTTCCTACACT+TGG + contig244end:28972-28991 MsG0080048211.01.T01:intron 40.0%
GCTTAACTCTTTGGATCAAC+AGG + contig244end:28515-28534 MsG0080048211.01.T01:CDS 40.0%
GGATCAACAGGGTAATTGTA+TGG + contig244end:28527-28546 MsG0080048211.01.T01:intron 40.0%
GTACCTTGCGAATCAAATAG+AGG - contig244end:27800-27819 None:intergenic 40.0%
GTTGAGTGAACTTTGTCTTG+AGG + contig244end:28834-28853 MsG0080048211.01.T01:intron 40.0%
TAAAGCTTATGGAAGAAGGG+AGG - contig244end:28755-28774 None:intergenic 40.0%
TAAAGCTTCTTGTGTACTGC+AGG + contig244end:29295-29314 MsG0080048211.01.T01:intron 40.0%
TGAACGTGGTAGAAAGAAAG+AGG + contig244end:26468-26487 MsG0080048211.01.T01:five_prime_UTR 40.0%
TGATGAAGAGAATCTGAGGA+AGG + contig244end:29397-29416 MsG0080048211.01.T01:CDS 40.0%
TGCTGTTGTCACTATTCTCA+AGG + contig244end:27912-27931 MsG0080048211.01.T01:intron 40.0%
! AGGATTAGGAACTGATTCAC+AGG - contig244end:28412-28431 None:intergenic 40.0%
! GAATCTGAGGAAGGTTTTTG+AGG + contig244end:29406-29425 MsG0080048211.01.T01:CDS 40.0%
! TCGCAAGGTGTTTGTCAAAA+TGG + contig244end:26832-26851 MsG0080048211.01.T01:intron 40.0%
!!! AGCTGCTCTTTGTTTTGATG+AGG - contig244end:27292-27311 None:intergenic 40.0%
AACTGCTGGAACGAGTTATG+TGG - contig244end:29585-29604 None:intergenic 45.0%
ACAATGAGCAATTCTGCTGC+CGG + contig244end:27264-27283 MsG0080048211.01.T01:exon 45.0%
ACACATCATCTACGTGAACG+TGG + contig244end:26454-26473 MsG0080048211.01.T01:five_prime_UTR 45.0%
CAGCACATGATGTATGGCTT+CGG + contig244end:27452-27471 MsG0080048211.01.T01:CDS 45.0%
CAGGAAGTGGCTAATTCAAG+TGG - contig244end:27569-27588 None:intergenic 45.0%
CCAGAAAACTAACCTCTGCA+AGG - contig244end:28926-28945 None:intergenic 45.0%
GAAAACTAACCTCTGCAAGG+AGG - contig244end:28923-28942 None:intergenic 45.0%
GATCGTGTCTGTGTATGTGT+CGG - contig244end:26929-26948 None:intergenic 45.0%
TCAACCATTCATCCTTCTGC+TGG - contig244end:29440-29459 None:intergenic 45.0%
TCACTGACACCTCTGATTGA+AGG - contig244end:27043-27062 None:intergenic 45.0%
TGAACCAGCAGAAGGATGAA+TGG + contig244end:29433-29452 MsG0080048211.01.T01:exon 45.0%
TTAGCCACTTCCTGAAAGTG+TGG + contig244end:27575-27594 MsG0080048211.01.T01:intron 45.0%
TTAGTCTAAGTGCCATGGAG+TGG + contig244end:27673-27692 MsG0080048211.01.T01:intron 45.0%
!! CAGAGGTGTCAGTGATACAA+CGG + contig244end:27048-27067 MsG0080048211.01.T01:intron 45.0%
!! TCTGAGGAAGGTTTTTGAGG+TGG + contig244end:29409-29428 MsG0080048211.01.T01:CDS 45.0%
!!! CCTTGCAGAGGTTAGTTTTC+TGG + contig244end:28923-28942 MsG0080048211.01.T01:intron 45.0%
AACCTTTCCTACACTTGGCC+AGG + contig244end:28977-28996 MsG0080048211.01.T01:intron 50.0%
ACTTCATCCAAGCGCAGAAG+AGG + contig244end:27330-27349 MsG0080048211.01.T01:CDS 50.0%
CACTATAATAGCCGGACTGC+TGG + contig244end:28587-28606 MsG0080048211.01.T01:intron 50.0%
GAAAACTCCTCTTCTGCGCT+TGG - contig244end:27340-27359 None:intergenic 50.0%
TATGTCACAGTGGCCTGCAA+TGG + contig244end:28215-28234 MsG0080048211.01.T01:intron 50.0%
TCCATAAGCGCCACACTTTC+AGG - contig244end:27588-27607 None:intergenic 50.0%
TCTAAGTGCCATGGAGTGGT+TGG + contig244end:27677-27696 MsG0080048211.01.T01:intron 50.0%
! CTCTCGTCACCTTTTCTTCG+AGG + contig244end:26499-26518 MsG0080048211.01.T01:five_prime_UTR 50.0%
! TCCTGAAAGTGTGGCGCTTA+TGG + contig244end:27584-27603 MsG0080048211.01.T01:CDS 50.0%
! TGAAGTGTCTGGAGGTTGTG+AGG - contig244end:27316-27335 None:intergenic 50.0%
!! TTGACCATGGTGCTGTCCTT+TGG + contig244end:28662-28681 MsG0080048211.01.T01:intron 50.0%
AGCGCCACACTTTCAGGAAG+TGG - contig244end:27582-27601 None:intergenic 55.0%
ATGTTGCAGCCTCCTTGCAG+AGG + contig244end:28911-28930 MsG0080048211.01.T01:intron 55.0%
CAGAACTCCTGGCCAAGTGT+AGG - contig244end:28987-29006 None:intergenic 55.0%
CAGAACTGTGTCCAGCAGTC+CGG - contig244end:28601-28620 None:intergenic 55.0%
CCTGACACGCCTTCAATCAG+AGG + contig244end:27031-27050 MsG0080048211.01.T01:intron 55.0%
CTCCTGGCCAAGTGTAGGAA+AGG - contig244end:28982-29001 None:intergenic 55.0%
GACAAAGGCCAACCACTCCA+TGG - contig244end:27688-27707 None:intergenic 55.0%
GAGGGGGAAGCTATCAGTTG+AGG + contig244end:27770-27789 MsG0080048211.01.T01:CDS 55.0%
GAGGTGGATGAACCAGCAGA+AGG + contig244end:29425-29444 MsG0080048211.01.T01:CDS 55.0%
TGGTCCAAAGGACAGCACCA+TGG - contig244end:28669-28688 None:intergenic 55.0%
! CCTCTGATTGAAGGCGTGTC+AGG - contig244end:27034-27053 None:intergenic 55.0%
! CGCTTGGATGAAGTGTCTGG+AGG - contig244end:27324-27343 None:intergenic 55.0%
! CTGCGCTTGGATGAAGTGTC+TGG - contig244end:27327-27346 None:intergenic 55.0%
GTCACAGTGGCCTGCAATGG+TGG + contig244end:28218-28237 MsG0080048211.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
contig244end gene 26452 29658 26452 ID=MsG0080048211.01;Name=MsG0080048211.01
contig244end mRNA 26452 29658 26452 ID=MsG0080048211.01.T01;Parent=MsG0080048211.01;Name=MsG0080048211.01.T01;_AED=0.39;_eAED=0.47;_QI=90|0.66|0.85|1|0.66|0.57|7|207|175
contig244end exon 26452 26520 26452 ID=MsG0080048211.01.T01:exon:13463;Parent=MsG0080048211.01.T01
contig244end exon 27246 27369 27246 ID=MsG0080048211.01.T01:exon:13464;Parent=MsG0080048211.01.T01
contig244end exon 27450 27487 27450 ID=MsG0080048211.01.T01:exon:13465;Parent=MsG0080048211.01.T01
contig244end exon 27579 27638 27579 ID=MsG0080048211.01.T01:exon:13466;Parent=MsG0080048211.01.T01
contig244end exon 27741 27815 27741 ID=MsG0080048211.01.T01:exon:13467;Parent=MsG0080048211.01.T01
contig244end exon 28421 28537 28421 ID=MsG0080048211.01.T01:exon:13468;Parent=MsG0080048211.01.T01
contig244end exon 29317 29658 29317 ID=MsG0080048211.01.T01:exon:13469;Parent=MsG0080048211.01.T01
contig244end five_prime_UTR 26452 26520 26452 ID=MsG0080048211.01.T01:five_prime_utr;Parent=MsG0080048211.01.T01
contig244end five_prime_UTR 27246 27266 27246 ID=MsG0080048211.01.T01:five_prime_utr;Parent=MsG0080048211.01.T01
contig244end CDS 27267 27369 27267 ID=MsG0080048211.01.T01:cds;Parent=MsG0080048211.01.T01
contig244end CDS 27450 27487 27450 ID=MsG0080048211.01.T01:cds;Parent=MsG0080048211.01.T01
contig244end CDS 27579 27638 27579 ID=MsG0080048211.01.T01:cds;Parent=MsG0080048211.01.T01
contig244end CDS 27741 27815 27741 ID=MsG0080048211.01.T01:cds;Parent=MsG0080048211.01.T01
contig244end CDS 28421 28537 28421 ID=MsG0080048211.01.T01:cds;Parent=MsG0080048211.01.T01
contig244end CDS 29317 29451 29317 ID=MsG0080048211.01.T01:cds;Parent=MsG0080048211.01.T01
contig244end three_prime_UTR 29452 29658 29452 ID=MsG0080048211.01.T01:three_prime_utr;Parent=MsG0080048211.01.T01
Gene Sequence

>MsG0080048211.01.T01

ATGAGCAATTCTGCTGCCGGAACCTCATCAAAACAAAGAGCAGCTTCCTCACAACCTCCAGACACTTCATCCAAGCGCAGAAGAGGAGTTTTCCAAAGAGAATTGCAGCACATGATGTATGGCTTCGGAGATGATCCTAATCCACTTCCTGAAAGTGTGGCGCTTATGGATGACATCGTTGTAGAATATATTACAGAACTGGTACATAAAGCACAAGATATTGGATCACAGAGGGGGAAGCTATCAGTTGAGGATTTCCTCTATTTGATTCGCAAGTTCCTAATCCTAAATAACCTGTATTGGTTCAATAATCGGTTATCTAATGTATCTGTCAAATCTGAGGTTAGGATCTATAATCTTGCCTTAAATCGCTTAACTCTTTGGATCAACAGGGATATGCCAAAACTTAACCGTTGTACAGAACTGTTGTCTATGAATGAAGAGCTAAAACAAGCAAGGAAACTTTTTGAACATGATGAAGAGAATCTGAGGAAGGTTTTTGAGGTGGATGAACCAGCAGAAGGATGA
Protein sequence

>MsG0080048211.01.T01

MSNSAAGTSSKQRAASSQPPDTSSKRRRGVFQRELQHMMYGFGDDPNPLPESVALMDDIVVEYITELVHKAQDIGSQRGKLSVEDFLYLIRKFLILNNLYWFNNRLSNVSVKSEVRIYNLALNRLTLWINRDMPKLNRCTELLSMNEELKQARKLFEHDEENLRKVFEVDEPAEG*