AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180001978.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001978.01.T01 MTR_1g047200 75.661 189 10 2 23 211 51 203 1.39e-83 247
MsG0180001978.01.T01 MTR_0085s0030 64.516 124 26 2 28 148 52 160 2.71e-40 135
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001978.01.T01 AT4G20280 53.416 161 39 2 51 211 86 210 8.75e-53 169
MsG0180001978.01.T01 AT1G20000 46.591 176 47 4 37 211 75 204 1.87e-41 140

Find 40 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAATTACGATTGCAGTATC+TGG 0.180287 1:-30160663 MsG0180001978.01.T01:CDS
ATTCTTGGTGATTGTGTTAT+TGG 0.201346 1:+30162339 None:intergenic
ATCTTCTTCATCTTTATTCT+TGG 0.203770 1:+30162324 None:intergenic
CCAGATACTGCAATCGTAAT+TGG 0.234841 1:+30160663 None:intergenic
TGCTGGTGACCCTCATAAAA+TGG 0.315270 1:-30162254 MsG0180001978.01.T01:CDS
TGATGAAGAAGAAGATAATA+TGG 0.321458 1:-30162302 MsG0180001978.01.T01:CDS
TTCTTGGTGATTGTGTTATT+GGG 0.353089 1:+30162340 None:intergenic
CTCAGATGACAGGGCCGAAT+TGG 0.356800 1:+30160387 None:intergenic
ATGAAAGAGAGGAAGGAAAC+TGG 0.371797 1:-30160411 MsG0180001978.01.T01:CDS
CAGTTATTAGCAAGCATCAC+TGG 0.391735 1:-30160705 MsG0180001978.01.T01:CDS
TCCAGAAAGCTAATATGAAA+AGG 0.416360 1:-30162074 MsG0180001978.01.T01:CDS
GAACAACCTCGAGGCTCTTT+CGG 0.424153 1:-30160320 MsG0180001978.01.T01:CDS
CCTCGAGGCTCTTTCGGTAA+TGG 0.442324 1:-30160314 None:intergenic
GTGGGTGAAGTTGTGGAAAC+AGG 0.442581 1:-30160624 MsG0180001978.01.T01:CDS
CAATTACGATTGCAGTATCT+GGG 0.454042 1:-30160662 MsG0180001978.01.T01:CDS
ATGTGCTTCTCTCAGATGAC+AGG 0.458430 1:+30160377 None:intergenic
TATGAGTCATTTCGTAGAGC+TGG 0.466823 1:-30162099 MsG0180001978.01.T01:CDS
CATAGACGACTAAAGCTTGA+AGG 0.473602 1:-30160357 MsG0180001978.01.T01:CDS
TTTGTATGAGGATGTTGTGT+TGG 0.475150 1:-30161913 MsG0180001978.01.T01:intron
GATTGTGTTATTGGGAGAAA+TGG 0.481276 1:+30162348 None:intergenic
GGAAGGAAACTGGACCAATT+CGG 0.484795 1:-30160401 MsG0180001978.01.T01:CDS
CTTGCTAAGTTTCCTACTGC+TGG 0.491386 1:-30162271 MsG0180001978.01.T01:CDS
AACAATTATGAAAGAGAGGA+AGG 0.500999 1:-30160418 MsG0180001978.01.T01:CDS
CCAGAAAGCTAATATGAAAA+GGG 0.511038 1:-30162073 MsG0180001978.01.T01:intron
CCTCACAATTGCTATCTCCT+TGG 0.511105 1:+30162812 None:intergenic
TCATAATTGTTCTAGCTGCA+AGG 0.546091 1:+30160430 None:intergenic
AGGATGTTGTGTTGGATGTG+TGG 0.548943 1:-30161905 MsG0180001978.01.T01:intron
TGGGATTGCAAAAGTGTTCG+TGG 0.557351 1:-30160643 MsG0180001978.01.T01:CDS
GATGCTTGTAAAGGAAAACT+AGG 0.561031 1:+30160589 None:intergenic
GAAGCATGTGATGCTTGTAA+AGG 0.566783 1:+30160580 None:intergenic
CTAGAACAATTATGAAAGAG+AGG 0.568793 1:-30160422 MsG0180001978.01.T01:CDS
AGTTATTAGCAAGCATCACT+GGG 0.579490 1:-30160704 MsG0180001978.01.T01:CDS
TTATGAGGGTCACCAGCAGT+AGG 0.599687 1:+30162259 None:intergenic
GTCACGATCTCACATCTCAA+AGG 0.604098 1:+30162834 None:intergenic
AGTGTTCGTGGGTGAAGTTG+TGG 0.641295 1:-30160631 MsG0180001978.01.T01:CDS
GAGATGTGAGATCGTGACCA+AGG 0.651917 1:-30162829 MsG0180001978.01.T01:CDS
TGTGCTTCTCTCAGATGACA+GGG 0.664896 1:+30160378 None:intergenic
GGGATTGCAAAAGTGTTCGT+GGG 0.672490 1:-30160642 MsG0180001978.01.T01:CDS
CCATTACCGAAAGAGCCTCG+AGG 0.733132 1:+30160314 None:intergenic
TCTTTAAGAGAACAACCTCG+AGG 0.744964 1:-30160329 MsG0180001978.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAACAAAAAATAAAATTA+GGG + Chr1:30160992-30161011 None:intergenic 10.0%
!! AATTCCAATTATATATTAAA+CGG + Chr1:30162605-30162624 None:intergenic 10.0%
!! CAACAACAAAAAATAAAATT+AGG + Chr1:30160993-30161012 None:intergenic 15.0%
!! TGAAGTTCTTATAAAATATA+TGG + Chr1:30160741-30160760 None:intergenic 15.0%
!!! ATTTTTTGTTGTTGATTTTT+AGG - Chr1:30160999-30161018 MsG0180001978.01.T01:intron 15.0%
!!! TTAATCTTGTTATAAGTTAA+TGG - Chr1:30161133-30161152 MsG0180001978.01.T01:intron 15.0%
!!! TTTCTTAATTTTTTTGTATG+AGG - Chr1:30161235-30161254 MsG0180001978.01.T01:intron 15.0%
!!! TTTTTATTTTTTTTGATGCT+AGG - Chr1:30162149-30162168 MsG0180001978.01.T01:intron 15.0%
!!! TTTTTTGTTGTTGATTTTTA+GGG - Chr1:30161000-30161019 MsG0180001978.01.T01:intron 15.0%
!! AGTTCTTATAAAATATATGG+AGG + Chr1:30160738-30160757 None:intergenic 20.0%
!! ATAACAACATGAAAAAACAA+TGG + Chr1:30160592-30160611 None:intergenic 20.0%
!! CAAAAAATAAAAACAACAGT+TGG + Chr1:30161873-30161892 None:intergenic 20.0%
!! GTTCTTATAAAATATATGGA+GGG + Chr1:30160737-30160756 None:intergenic 20.0%
!! TATTTATAAACAGCTACAAT+TGG - Chr1:30162348-30162367 MsG0180001978.01.T01:CDS 20.0%
!! TTGTTAAATAAAGAGGATTT+GGG - Chr1:30161743-30161762 MsG0180001978.01.T01:intron 20.0%
!!! AAAAGCATAAATAAAACAAG+TGG - Chr1:30160434-30160453 MsG0180001978.01.T01:CDS 20.0%
!!! ATTTGAGTTGTTAAATAAAG+AGG - Chr1:30161736-30161755 MsG0180001978.01.T01:intron 20.0%
! ATCTTCTTCATCTTTATTCT+TGG + Chr1:30160839-30160858 None:intergenic 25.0%
! ATTAGAGAGAATATATGACT+TGG - Chr1:30160659-30160678 MsG0180001978.01.T01:CDS 25.0%
! GCTTCCGTTTAATATATAAT+TGG - Chr1:30162598-30162617 MsG0180001978.01.T01:intron 25.0%
! GTTGTTAAATAAAGAGGATT+TGG - Chr1:30161742-30161761 MsG0180001978.01.T01:intron 25.0%
! TATAACCTATAGTGAAAAGA+CGG - Chr1:30161329-30161348 MsG0180001978.01.T01:intron 25.0%
! TGATGAAGAAGAAGATAATA+TGG - Chr1:30160858-30160877 MsG0180001978.01.T01:intron 25.0%
! TGTTAAATAAAGAGGATTTG+GGG - Chr1:30161744-30161763 MsG0180001978.01.T01:intron 25.0%
! TTGGAATTAGAAAAATGTGA+AGG - Chr1:30162617-30162636 MsG0180001978.01.T01:intron 25.0%
! TTTATTGAAATAACAACCGT+TGG + Chr1:30161906-30161925 None:intergenic 25.0%
!! ATTTCTCTAATAGTTTTCCT+AGG + Chr1:30161472-30161491 None:intergenic 25.0%
!! CTTTGTTTGTTTGATATTCT+GGG + Chr1:30162105-30162124 None:intergenic 25.0%
!! TTGATTTGAATCAACATTGT+GGG + Chr1:30160531-30160550 None:intergenic 25.0%
!! TTTTTGACAAAGAAAACCAA+CGG - Chr1:30161887-30161906 MsG0180001978.01.T01:intron 25.0%
!!! AGAATATATGACTTGGTATT+AGG - Chr1:30160666-30160685 MsG0180001978.01.T01:CDS 25.0%
!!! ATTTTTTTTGATGCTAGGTT+AGG - Chr1:30162154-30162173 MsG0180001978.01.T01:intron 25.0%
!!! CCATGTTTTACTTTTTCTAT+GGG - Chr1:30162038-30162057 MsG0180001978.01.T01:intron 25.0%
!!! TAAAAGATCAGGATTAGTTA+TGG - Chr1:30161683-30161702 MsG0180001978.01.T01:intron 25.0%
!!! TCCATGTTTTACTTTTTCTA+TGG - Chr1:30162037-30162056 MsG0180001978.01.T01:intron 25.0%
!!! TTACTTTTTCTATGGGTAAT+TGG - Chr1:30162045-30162064 MsG0180001978.01.T01:intron 25.0%
!!! TTTGATTTGAATCAACATTG+TGG + Chr1:30160532-30160551 None:intergenic 25.0%
AAAGTAAGTGTACAAATGTC+AGG + Chr1:30161212-30161231 None:intergenic 30.0%
AACAATTATGAAAGAGAGGA+AGG - Chr1:30162742-30162761 MsG0180001978.01.T01:intron 30.0%
AAGAACCAAGAAGAGTAAAT+CGG - Chr1:30161410-30161429 MsG0180001978.01.T01:intron 30.0%
AGAAAGAGAACTCAACAAAA+GGG + Chr1:30160561-30160580 None:intergenic 30.0%
AGAATTGGAGTTAAAAGATC+AGG - Chr1:30161672-30161691 MsG0180001978.01.T01:intron 30.0%
CATAGCTCAATTAATGTTGA+AGG + Chr1:30162192-30162211 None:intergenic 30.0%
CCAGAAAGCTAATATGAAAA+GGG - Chr1:30161087-30161106 MsG0180001978.01.T01:intron 30.0%
CCCATAGAAAAAGTAAAACA+TGG + Chr1:30162041-30162060 None:intergenic 30.0%
CTAGAACAATTATGAAAGAG+AGG - Chr1:30162738-30162757 MsG0180001978.01.T01:intron 30.0%
CTTCAACATTAATTGAGCTA+TGG - Chr1:30162190-30162209 MsG0180001978.01.T01:intron 30.0%
GGAATTAACAGATGTCTTTA+AGG - Chr1:30161794-30161813 MsG0180001978.01.T01:intron 30.0%
GGTGTTAAATTCTGGATATA+GGG - Chr1:30161642-30161661 MsG0180001978.01.T01:intron 30.0%
GTACTGTTTCCATTAATCAA+AGG - Chr1:30162699-30162718 MsG0180001978.01.T01:intron 30.0%
TAAAATATATGGAGGGAGTA+AGG + Chr1:30160730-30160749 None:intergenic 30.0%
TAGAAAGAGAACTCAACAAA+AGG + Chr1:30160562-30160581 None:intergenic 30.0%
TCCAGAAAGCTAATATGAAA+AGG - Chr1:30161086-30161105 MsG0180001978.01.T01:intron 30.0%
TGATGAGTTAGATGGAAATA+TGG - Chr1:30161513-30161532 MsG0180001978.01.T01:intron 30.0%
! AACTTTTCCCTAATCAGTTT+GGG + Chr1:30162234-30162253 None:intergenic 30.0%
! AATTGGAATCGAGATTTTCT+GGG + Chr1:30162483-30162502 None:intergenic 30.0%
! ATTCTGGGAAGAATTTAGAA+TGG + Chr1:30162090-30162109 None:intergenic 30.0%
! ATTCTTGGTGATTGTGTTAT+TGG + Chr1:30160824-30160843 None:intergenic 30.0%
! CTTTTATCCCAAACTGATTA+GGG - Chr1:30162224-30162243 MsG0180001978.01.T01:intron 30.0%
! GATAGAGTACCTTTGATTAA+TGG + Chr1:30162711-30162730 None:intergenic 30.0%
! TAACTTTTCCCTAATCAGTT+TGG + Chr1:30162235-30162254 None:intergenic 30.0%
! TAATTGGAATCGAGATTTTC+TGG + Chr1:30162484-30162503 None:intergenic 30.0%
! TCTTTTATCCCAAACTGATT+AGG - Chr1:30162223-30162242 MsG0180001978.01.T01:intron 30.0%
! TGGTATATGATAGAGCATTA+TGG - Chr1:30161533-30161552 MsG0180001978.01.T01:intron 30.0%
! TGGTGTTAAATTCTGGATAT+AGG - Chr1:30161641-30161660 MsG0180001978.01.T01:intron 30.0%
! TTCTTGGTGATTGTGTTATT+GGG + Chr1:30160823-30160842 None:intergenic 30.0%
!! AAACTCGTGTTTTGTTATGA+TGG - Chr1:30161923-30161942 MsG0180001978.01.T01:intron 30.0%
!! AACTCGTGTTTTGTTATGAT+GGG - Chr1:30161924-30161943 MsG0180001978.01.T01:intron 30.0%
!! GCTTTGTTTGTTTGATATTC+TGG + Chr1:30162106-30162125 None:intergenic 30.0%
ACTTGGTATTAGGAAAGAGT+AGG - Chr1:30160676-30160695 MsG0180001978.01.T01:CDS 35.0%
AGTCAAATCATTAGAGGCAA+AGG - Chr1:30161442-30161461 MsG0180001978.01.T01:intron 35.0%
AGTTATTAGCAAGCATCACT+GGG - Chr1:30162456-30162475 MsG0180001978.01.T01:intron 35.0%
ATTAGGAAAGAGTAGGCTTT+AGG - Chr1:30160683-30160702 MsG0180001978.01.T01:CDS 35.0%
ATTTGAATCAACATTGTGGG+TGG + Chr1:30160528-30160547 None:intergenic 35.0%
CAAACCTTATCTCACTAAGT+GGG + Chr1:30161583-30161602 None:intergenic 35.0%
CAATTACGATTGCAGTATCT+GGG - Chr1:30162498-30162517 MsG0180001978.01.T01:intron 35.0%
CATGTTGTTATACAAGAGCA+TGG - Chr1:30160601-30160620 MsG0180001978.01.T01:CDS 35.0%
CTTGAGATTGATGAGTTAGA+TGG - Chr1:30161505-30161524 MsG0180001978.01.T01:intron 35.0%
GAACTTCACTTATTGCTTCT+AGG - Chr1:30160753-30160772 MsG0180001978.01.T01:intron 35.0%
GAGAGTAGTCAAATCATTAG+AGG - Chr1:30161436-30161455 MsG0180001978.01.T01:intron 35.0%
GATGCTTGTAAAGGAAAACT+AGG + Chr1:30162574-30162593 None:intergenic 35.0%
TATTACATGTCAGTGCTTCA+TGG - Chr1:30161621-30161640 MsG0180001978.01.T01:intron 35.0%
TATTAGTCCTCTCTCTTACA+GGG + Chr1:30162410-30162429 None:intergenic 35.0%
TCGTTCTTAGAGGAAACTAA+AGG - Chr1:30161773-30161792 MsG0180001978.01.T01:intron 35.0%
TTTGTATGAGGATGTTGTGT+TGG - Chr1:30161247-30161266 MsG0180001978.01.T01:intron 35.0%
! CTTCTGATTGAAAGTGTGTT+CGG - Chr1:30161183-30161202 MsG0180001978.01.T01:intron 35.0%
! GATTGTGTTATTGGGAGAAA+TGG + Chr1:30160815-30160834 None:intergenic 35.0%
! GGAAGAATTTAGAATGGTCA+CGG + Chr1:30162084-30162103 None:intergenic 35.0%
! GTTATACAAGAGCATGGTAT+TGG - Chr1:30160607-30160626 MsG0180001978.01.T01:CDS 35.0%
! TCATAATTGTTCTAGCTGCA+AGG + Chr1:30162733-30162752 None:intergenic 35.0%
! TGCTTCATGGTGTTAAATTC+TGG - Chr1:30161634-30161653 MsG0180001978.01.T01:intron 35.0%
!! CTGTTATTTCAAAACCAGAG+AGG + Chr1:30162380-30162399 None:intergenic 35.0%
!! GCATCTTAGCCATTTTATGA+GGG + Chr1:30160918-30160937 None:intergenic 35.0%
!! TGCATCTTAGCCATTTTATG+AGG + Chr1:30160919-30160938 None:intergenic 35.0%
!!! CCCTTTTCATATTAGCTTTC+TGG + Chr1:30161090-30161109 None:intergenic 35.0%
!!! CTCTCTGGTTTTGAAATAAC+AGG - Chr1:30162378-30162397 MsG0180001978.01.T01:CDS 35.0%
AAAGTAGGCTTAGGTGGTTT+GGG - Chr1:30161355-30161374 MsG0180001978.01.T01:intron 40.0%
AAATTCTGGATATAGGGCTG+TGG - Chr1:30161648-30161667 MsG0180001978.01.T01:intron 40.0%
AACCTATAGTGAAAAGACGG+TGG - Chr1:30161332-30161351 MsG0180001978.01.T01:intron 40.0%
ACCAAGAAGAGTAAATCGGA+TGG - Chr1:30161414-30161433 MsG0180001978.01.T01:intron 40.0%
AGAGCGAAATTCAAACAACC+AGG + Chr1:30161820-30161839 None:intergenic 40.0%
ATATAGGGCTGTGGAAGAAT+TGG - Chr1:30161657-30161676 MsG0180001978.01.T01:intron 40.0%
ATGAAAGAGAGGAAGGAAAC+TGG - Chr1:30162749-30162768 MsG0180001978.01.T01:intron 40.0%
CAGTTATTAGCAAGCATCAC+TGG - Chr1:30162455-30162474 MsG0180001978.01.T01:intron 40.0%
CATAGACGACTAAAGCTTGA+AGG - Chr1:30162803-30162822 MsG0180001978.01.T01:intron 40.0%
CCAAACCTTATCTCACTAAG+TGG + Chr1:30161584-30161603 None:intergenic 40.0%
CCAATTACGATTGCAGTATC+TGG - Chr1:30162497-30162516 MsG0180001978.01.T01:intron 40.0%
CCACTTAGTGAGATAAGGTT+TGG - Chr1:30161581-30161600 MsG0180001978.01.T01:intron 40.0%
CCAGATACTGCAATCGTAAT+TGG + Chr1:30162500-30162519 None:intergenic 40.0%
CTCAGAAACATGATATGCAC+AGG + Chr1:30162665-30162684 None:intergenic 40.0%
GAAGCATGTGATGCTTGTAA+AGG + Chr1:30162583-30162602 None:intergenic 40.0%
GTATTAGTCCTCTCTCTTAC+AGG + Chr1:30162411-30162430 None:intergenic 40.0%
TATGAGTCATTTCGTAGAGC+TGG - Chr1:30161061-30161080 MsG0180001978.01.T01:intron 40.0%
TCCATCCGATTTACTCTTCT+TGG + Chr1:30161418-30161437 None:intergenic 40.0%
TGATCCCACTTAGTGAGATA+AGG - Chr1:30161576-30161595 MsG0180001978.01.T01:intron 40.0%
! CTGTGCATATCATGTTTCTG+AGG - Chr1:30162663-30162682 MsG0180001978.01.T01:intron 40.0%
! TCTTTAAGAGAACAACCTCG+AGG - Chr1:30162831-30162850 MsG0180001978.01.T01:CDS 40.0%
! TTCCACCGTCTTTTCACTAT+AGG + Chr1:30161337-30161356 None:intergenic 40.0%
!! TAACAGATGTCTTTAAGGCC+TGG - Chr1:30161799-30161818 MsG0180001978.01.T01:intron 40.0%
ACTAAGTGGGATCAGCTACA+TGG + Chr1:30161570-30161589 None:intergenic 45.0%
AGGATGTTGTGTTGGATGTG+TGG - Chr1:30161255-30161274 MsG0180001978.01.T01:intron 45.0%
ATGTGCTTCTCTCAGATGAC+AGG + Chr1:30162786-30162805 None:intergenic 45.0%
CCAAGGAGATAGCAATTGTG+AGG - Chr1:30160348-30160367 MsG0180001978.01.T01:CDS 45.0%
CCTCACAATTGCTATCTCCT+TGG + Chr1:30160351-30160370 None:intergenic 45.0%
CTTGCTAAGTTTCCTACTGC+TGG - Chr1:30160889-30160908 MsG0180001978.01.T01:intron 45.0%
GAAAGTAGGCTTAGGTGGTT+TGG - Chr1:30161354-30161373 MsG0180001978.01.T01:intron 45.0%
GATGTGTGGTAAGACTAGAC+GGG - Chr1:30161269-30161288 MsG0180001978.01.T01:intron 45.0%
GGAAGGAAACTGGACCAATT+CGG - Chr1:30162759-30162778 MsG0180001978.01.T01:intron 45.0%
GGATGTGTGGTAAGACTAGA+CGG - Chr1:30161268-30161287 MsG0180001978.01.T01:intron 45.0%
GGGGTTTGAATCGTTCTTAG+AGG - Chr1:30161763-30161782 MsG0180001978.01.T01:intron 45.0%
GTCACGATCTCACATCTCAA+AGG + Chr1:30160329-30160348 None:intergenic 45.0%
GTGAAAAGACGGTGGAAAGT+AGG - Chr1:30161340-30161359 MsG0180001978.01.T01:intron 45.0%
TCAACAATTGCACGTGTCTG+TGG + Chr1:30162297-30162316 None:intergenic 45.0%
TGCTGGTGACCCTCATAAAA+TGG - Chr1:30160906-30160925 MsG0180001978.01.T01:intron 45.0%
TGTGCTTCTCTCAGATGACA+GGG + Chr1:30162785-30162804 None:intergenic 45.0%
TTAACGTGAGATGCTCACCA+CGG - Chr1:30162004-30162023 MsG0180001978.01.T01:intron 45.0%
TTAGAGGCAAAGGAAGACCT+AGG - Chr1:30161452-30161471 MsG0180001978.01.T01:intron 45.0%
!! ACAATTGGCTTGATCCTCTC+TGG - Chr1:30162363-30162382 MsG0180001978.01.T01:CDS 45.0%
!! GGGATTGCAAAAGTGTTCGT+GGG - Chr1:30162518-30162537 MsG0180001978.01.T01:intron 45.0%
!! GTAGGCTTTAGGAGAGTTCT+TGG - Chr1:30160694-30160713 MsG0180001978.01.T01:CDS 45.0%
!! TGGGATTGCAAAAGTGTTCG+TGG - Chr1:30162517-30162536 MsG0180001978.01.T01:intron 45.0%
ACAATTGCACGTGTCTGTGG+AGG + Chr1:30162294-30162313 None:intergenic 50.0%
AGACGGTGGAAAGTAGGCTT+AGG - Chr1:30161346-30161365 MsG0180001978.01.T01:intron 50.0%
AGTGTTCGTGGGTGAAGTTG+TGG - Chr1:30162529-30162548 MsG0180001978.01.T01:intron 50.0%
GAACAACCTCGAGGCTCTTT+CGG - Chr1:30162840-30162859 MsG0180001978.01.T01:CDS 50.0%
GAGATGTGAGATCGTGACCA+AGG - Chr1:30160331-30160350 MsG0180001978.01.T01:CDS 50.0%
GATACAGCCCTGTAAGAGAG+AGG - Chr1:30162400-30162419 MsG0180001978.01.T01:intron 50.0%
GTGGGTGAAGTTGTGGAAAC+AGG - Chr1:30162536-30162555 MsG0180001978.01.T01:intron 50.0%
TTATGAGGGTCACCAGCAGT+AGG + Chr1:30160904-30160923 None:intergenic 50.0%
!! ATGCTCACCACGGAAGTGTT+GGG - Chr1:30162014-30162033 MsG0180001978.01.T01:intron 50.0%
ACATGGACCCAACACTTCCG+TGG + Chr1:30162024-30162043 None:intergenic 55.0%
CGGTGGAAAGTAGGCTTAGG+TGG - Chr1:30161349-30161368 MsG0180001978.01.T01:intron 55.0%
CTCAGATGACAGGGCCGAAT+TGG + Chr1:30162776-30162795 None:intergenic 55.0%
! GATGCTCACCACGGAAGTGT+TGG - Chr1:30162013-30162032 MsG0180001978.01.T01:intron 55.0%
! TAGGTGGTTTGGGCGTGTAG+CGG - Chr1:30161365-30161384 MsG0180001978.01.T01:intron 55.0%
! AGGTGGTTTGGGCGTGTAGC+GGG - Chr1:30161366-30161385 MsG0180001978.01.T01:intron 60.0%
! GGTTTGGGCGTGTAGCGGGA+TGG - Chr1:30161370-30161389 MsG0180001978.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 30160317 30162865 30160317 ID=MsG0180001978.01;Name=MsG0180001978.01
Chr1 mRNA 30160317 30162865 30160317 ID=MsG0180001978.01.T01;Parent=MsG0180001978.01;Name=MsG0180001978.01.T01;_AED=0.50;_eAED=0.60;_QI=0|0|0|1|0.2|0.16|6|0|211
Chr1 exon 30162818 30162865 30162818 ID=MsG0180001978.01.T01:exon:19153;Parent=MsG0180001978.01.T01
Chr1 exon 30162244 30162407 30162244 ID=MsG0180001978.01.T01:exon:19152;Parent=MsG0180001978.01.T01
Chr1 exon 30162074 30162161 30162074 ID=MsG0180001978.01.T01:exon:19151;Parent=MsG0180001978.01.T01
Chr1 exon 30161906 30161925 30161906 ID=MsG0180001978.01.T01:exon:19150;Parent=MsG0180001978.01.T01
Chr1 exon 30160579 30160749 30160579 ID=MsG0180001978.01.T01:exon:19149;Parent=MsG0180001978.01.T01
Chr1 exon 30160317 30160461 30160317 ID=MsG0180001978.01.T01:exon:19148;Parent=MsG0180001978.01.T01
Chr1 CDS 30162818 30162865 30162818 ID=MsG0180001978.01.T01:cds;Parent=MsG0180001978.01.T01
Chr1 CDS 30162244 30162407 30162244 ID=MsG0180001978.01.T01:cds;Parent=MsG0180001978.01.T01
Chr1 CDS 30162074 30162161 30162074 ID=MsG0180001978.01.T01:cds;Parent=MsG0180001978.01.T01
Chr1 CDS 30161906 30161925 30161906 ID=MsG0180001978.01.T01:cds;Parent=MsG0180001978.01.T01
Chr1 CDS 30160579 30160749 30160579 ID=MsG0180001978.01.T01:cds;Parent=MsG0180001978.01.T01
Chr1 CDS 30160317 30160461 30160317 ID=MsG0180001978.01.T01:cds;Parent=MsG0180001978.01.T01
Gene Sequence

>MsG0180001978.01.T01

ATGCAAATACCTTTGAGATGTGAGATCGTGACCAAGGAGATAGCAATTGATGAAGTTTCAACTGTGAAGCACAAAAAAAATAACACCATTTCTCCCAATAACACAATCACCAAGAATAAAGATGAAGAAGATGATGAAGAAGAAGATAATATGGATGTTGAACTTGCTAAGTTTCCTACTGCTGGTGACCCTCATAAAATGGCTAAGATGCAGGCTATTTTGTCACAGTTTACTGAAGAACAAATGAGTAGATATGAGTCATTTCGTAGAGCTGGATTCCAGAAAGCTAATATGAAAAGGGATGTTGTGTTGGATGTGTGTTTCTTTTGTACACATGAACTGCAGTTATTAGCAAGCATCACTGGGACCCAGAAAATCTCGATTCCAATTACGATTGCAGTATCTGGGATTGCAAAAGTGTTCGTGGGTGAAGTTGTGGAAACAGGTAGATTAGTTTTCCTAGTTTTCCTTTACAAGCATCACATGCTTCCGTACTCTATCCTTGCAGCTAGAACAATTATGAAAGAGAGGAAGGAAACTGGACCAATTCGGCCCTGTCATCTGAGAGAAGCACATAGACGACTAAAGCTTGAAGGAAAAATCTTTAAGAGAACAACCTCGAGGCTCTTTCGGTAA
Protein sequence

>MsG0180001978.01.T01

MQIPLRCEIVTKEIAIDEVSTVKHKKNNTISPNNTITKNKDEEDDEEEDNMDVELAKFPTAGDPHKMAKMQAILSQFTEEQMSRYESFRRAGFQKANMKRDVVLDVCFFCTHELQLLASITGTQKISIPITIAVSGIAKVFVGEVVETGRLVFLVFLYKHHMLPYSILAARTIMKERKETGPIRPCHLREAHRRLKLEGKIFKRTTSRLFR*