Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039867.01.T01 | XP_003624232.1 | 98.718 | 78 | 1 | 0 | 1 | 78 | 1 | 78 | 3.29E-48 | 159 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047869.01 | MsG0780039867.01 | -0.808767 | 2.635008e-50 | 1.519549e-47 |
| MsG0080047944.01 | MsG0780039867.01 | -0.820752 | 5.810268e-53 | 4.637531e-50 |
| MsG0080047957.01 | MsG0780039867.01 | -0.841902 | 3.585007e-58 | 5.344261e-55 |
| MsG0080047959.01 | MsG0780039867.01 | -0.840102 | 1.064335e-57 | 1.500523e-54 |
| MsG0080048404.01 | MsG0780039867.01 | -0.808080 | 3.691625e-50 | 2.090974e-47 |
| MsG0080048461.01 | MsG0780039867.01 | -0.807256 | 5.525145e-50 | 3.062881e-47 |
| MsG0080048462.01 | MsG0780039867.01 | -0.825073 | 5.718272e-54 | 5.158484e-51 |
| MsG0080048463.01 | MsG0780039867.01 | -0.833048 | 6.671234e-56 | 7.594267e-53 |
| MsG0080048464.01 | MsG0780039867.01 | -0.837949 | 3.842003e-57 | 5.070064e-54 |
| MsG0080048660.01 | MsG0780039867.01 | -0.803845 | 2.869732e-49 | 1.457353e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0080048211.01 | MsG0780039867.01 | PPI |
| MsG0180004811.01 | MsG0780039867.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039867.01.T01 | MTR_7g080740 | 98.718 | 78 | 1 | 0 | 1 | 78 | 1 | 78 | 3.99e-52 | 159 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039867.01.T01 | AT4G31720 | 74.359 | 78 | 17 | 1 | 1 | 78 | 1 | 75 | 3.51e-36 | 119 |
| MsG0780039867.01.T01 | AT4G31720 | 74.359 | 78 | 17 | 1 | 1 | 78 | 1 | 75 | 3.51e-36 | 119 |
| MsG0780039867.01.T01 | AT4G31720 | 74.359 | 78 | 17 | 1 | 1 | 78 | 1 | 75 | 3.51e-36 | 119 |
Find 41 sgRNAs with CRISPR-Local
Find 131 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGGGCAAAATGAATATAC+TGG | 0.386364 | 7:+69905233 | None:intergenic |
| AAATTTGTTGCAGAAGTCGC+AGG | 0.516680 | 7:-69905265 | MsG0780039867.01.T01:CDS |
| AACAGGTGAGAGAGTCCGTG+AGG | 0.632159 | 7:+69907166 | None:intergenic |
| AACTAAGGAAGCTAGGAAAT+CGG | 0.431723 | 7:+69906235 | None:intergenic |
| AACTTGATTGCGGCTGATTC+TGG | 0.178719 | 7:+69906294 | None:intergenic |
| AATCAAGTTCAAGCGAAGGA+AGG | 0.545925 | 7:-69906280 | MsG0780039867.01.T01:CDS |
| ACAGGTGAGAGAGTCCGTGA+GGG | 0.623359 | 7:+69907167 | None:intergenic |
| AGGAACACGAGAACCCGATC+CGG | 0.560899 | 7:-69907252 | MsG0780039867.01.T01:five_prime_UTR |
| AGGACATTGAAAGCCACTCT+TGG | 0.525406 | 7:+69906076 | None:intergenic |
| AGTAGGAGTATAATCAACTA+AGG | 0.533067 | 7:+69906220 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGGGCAAAATGAATATAC+TGG | + | Chr7:69907299-69907318 | None:intergenic | 30.0% | |
| AAGAAAGATGCACATAGATA+AGG | + | Chr7:69906846-69906865 | None:intergenic | 30.0% | |
| ACAAACTCAAATAAATCCCA+AGG | + | Chr7:69907029-69907048 | None:intergenic | 30.0% | |
| ATTAGGCTTAATTGCATGTT+TGG | - | Chr7:69905670-69905689 | MsG0780039867.01.T01:intron | 30.0% | |
| CAAAAGAAAAATCACTCTTC+AGG | - | Chr7:69905257-69905276 | MsG0780039867.01.T01:CDS | 30.0% | |
| CAAATGTATCAGTGTTGTAT+TGG | - | Chr7:69906047-69906066 | MsG0780039867.01.T01:intron | 30.0% | |
| CATCTTTCTTACCCTTTATT+TGG | - | Chr7:69906856-69906875 | MsG0780039867.01.T01:intron | 30.0% | |
| CCAAGAATCAAACTTAAGTA+GGG | + | Chr7:69907181-69907200 | None:intergenic | 30.0% | |
| CTTGAAACTTTCAAAACGTA+AGG | - | Chr7:69905806-69905825 | MsG0780039867.01.T01:intron | 30.0% | |
| TAAACAGAAGTCAAGCAAAA+TGG | + | Chr7:69907147-69907166 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 69905222 | 69907329 | 69905222 | ID=MsG0780039867.01;Name=MsG0780039867.01 |
| Chr7 | mRNA | 69905222 | 69907329 | 69905222 | ID=MsG0780039867.01.T01;Parent=MsG0780039867.01;Name=MsG0780039867.01.T01;_AED=0.47;_eAED=0.51;_QI=212|1|0.75|1|0.66|0.5|4|0|86 |
| Chr7 | exon | 69905222 | 69905315 | 69905222 | ID=MsG0780039867.01.T01:exon:12921;Parent=MsG0780039867.01.T01 |
| Chr7 | exon | 69906065 | 69906129 | 69906065 | ID=MsG0780039867.01.T01:exon:12920;Parent=MsG0780039867.01.T01 |
| Chr7 | exon | 69906220 | 69906356 | 69906220 | ID=MsG0780039867.01.T01:exon:12919;Parent=MsG0780039867.01.T01 |
| Chr7 | exon | 69907153 | 69907329 | 69907153 | ID=MsG0780039867.01.T01:exon:12918;Parent=MsG0780039867.01.T01 |
| Chr7 | five_prime_UTR | 69907153 | 69907329 | 69907153 | ID=MsG0780039867.01.T01:five_prime_utr;Parent=MsG0780039867.01.T01 |
| Chr7 | five_prime_UTR | 69906322 | 69906356 | 69906322 | ID=MsG0780039867.01.T01:five_prime_utr;Parent=MsG0780039867.01.T01 |
| Chr7 | CDS | 69906220 | 69906321 | 69906220 | ID=MsG0780039867.01.T01:cds;Parent=MsG0780039867.01.T01 |
| Chr7 | CDS | 69906065 | 69906129 | 69906065 | ID=MsG0780039867.01.T01:cds;Parent=MsG0780039867.01.T01 |
| Chr7 | CDS | 69905222 | 69905315 | 69905222 | ID=MsG0780039867.01.T01:cds;Parent=MsG0780039867.01.T01 |
| Gene Sequence |
>MsG0780039867.01.T01 ATGAACCAGAATCAGCCGCAATCAAGTTCAAGCGAAGGAAGGGCAGATGATGATTCTGCACTATCCGATTTCCTAGCTTCCTTAGTTGATTATACTCCTACTATACCAGATGAGTTGGTGGAGCATTACTTGGCCAAGAGTGGCTTTCAATGTCCTGATGTGAGATTGACTAGATTGGTAGCTGTTGCCACTCAGAAATTTGTTGCAGAAGTCGCAGGCGATGCCCTCCAGTATATTCATTTTGCCCTTTCTATTGATTGA |
| Protein sequence |
>MsG0780039867.01.T01 MNQNQPQSSSSEGRADDDSALSDFLASLVDYTPTIPDELVEHYLAKSGFQCPDVRLTRLVAVATQKFVAEVAGDALQYIHFALSID* |
