Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048320.01.T01 | RID48993.1 | 85.507 | 69 | 8 | 1 | 4 | 72 | 56 | 122 | 1.75E-30 | 115 |
| MsG0080048320.01.T02 | KAG8086739.1 | 93.548 | 62 | 4 | 0 | 6 | 67 | 669 | 730 | 1.75E-29 | 121 |
| MsG0080048320.01.T03 | RID48993.1 | 87.931 | 58 | 4 | 1 | 1 | 55 | 65 | 122 | 2.08E-24 | 99.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048320.01.T01 | Q94C37 | 84.286 | 70 | 10 | 1 | 4 | 72 | 625 | 694 | 4.21E-33 | 123 |
| MsG0080048320.01.T02 | Q94C37 | 81.429 | 70 | 7 | 1 | 4 | 67 | 625 | 694 | 2.31E-30 | 115 |
| MsG0080048320.01.T03 | Q94C37 | 81.967 | 61 | 5 | 1 | 1 | 55 | 634 | 694 | 8.38E-26 | 102 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048320.01.T02 | A0A1J3IB77 | 86.364 | 66 | 7 | 1 | 4 | 67 | 14 | 79 | 2.03e-29 | 110 |
| MsG0080048320.01.T03 | A0A1J3IB77 | 89.474 | 57 | 4 | 1 | 1 | 55 | 23 | 79 | 2.10e-25 | 100 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048320.01 | MsG0180003741.01 | 0.802442 | 5.599295e-49 | 2.743131e-46 |
| MsG0080048320.01 | MsG0480020315.01 | 0.800936 | 1.140868e-48 | 5.379870e-46 |
| MsG0080048320.01 | MsG0680030908.01 | 0.804913 | 1.719055e-49 | 8.973676e-47 |
| MsG0080048320.01 | MsG0780041259.01 | 0.802594 | 5.208532e-49 | 2.561625e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048320.01.T01 | MTR_2g088470 | 78.378 | 74 | 11 | 1 | 4 | 72 | 637 | 710 | 5.79e-26 | 100 |
| MsG0080048320.01.T01 | MTR_7g076080 | 64.474 | 76 | 20 | 1 | 4 | 72 | 677 | 752 | 1.30e-25 | 99.8 |
| MsG0080048320.01.T01 | MTR_4g047800 | 78.481 | 79 | 7 | 1 | 4 | 72 | 629 | 707 | 1.81e-25 | 99.4 |
| MsG0080048320.01.T01 | MTR_8g432540 | 64.198 | 81 | 17 | 2 | 4 | 72 | 704 | 784 | 2.71e-25 | 98.6 |
| MsG0080048320.01.T01 | MTR_1g101280 | 58.025 | 81 | 22 | 1 | 4 | 72 | 648 | 728 | 1.05e-23 | 94.4 |
| MsG0080048320.01.T01 | MTR_5g005600 | 57.746 | 71 | 26 | 1 | 6 | 72 | 624 | 694 | 3.09e-20 | 84.3 |
| MsG0080048320.01.T01 | MTR_2g101720 | 40.506 | 79 | 38 | 1 | 3 | 72 | 689 | 767 | 1.64e-13 | 65.1 |
| MsG0080048320.01.T01 | MTR_0198s0030 | 38.095 | 84 | 37 | 2 | 4 | 72 | 646 | 729 | 9.21e-13 | 63.2 |
| MsG0080048320.01.T01 | MTR_6g477820 | 36.957 | 92 | 35 | 2 | 4 | 72 | 610 | 701 | 7.37e-12 | 60.5 |
| MsG0080048320.01.T01 | MTR_7g427710 | 43.902 | 82 | 25 | 3 | 4 | 68 | 538 | 615 | 2.21e-11 | 58.9 |
| MsG0080048320.01.T01 | MTR_0653s0010 | 43.902 | 82 | 25 | 3 | 4 | 68 | 14 | 91 | 7.34e-11 | 55.1 |
| MsG0080048320.01.T02 | MTR_2g088470 | 74.324 | 74 | 9 | 1 | 4 | 67 | 637 | 710 | 2.85e-26 | 101 |
| MsG0080048320.01.T02 | MTR_4g047800 | 74.684 | 79 | 5 | 1 | 4 | 67 | 629 | 707 | 9.59e-26 | 99.8 |
| MsG0080048320.01.T02 | MTR_7g076080 | 63.158 | 76 | 16 | 2 | 4 | 67 | 677 | 752 | 2.18e-23 | 93.2 |
| MsG0080048320.01.T02 | MTR_8g432540 | 58.025 | 81 | 17 | 1 | 4 | 67 | 704 | 784 | 1.37e-22 | 90.9 |
| MsG0080048320.01.T02 | MTR_1g101280 | 54.321 | 81 | 20 | 1 | 4 | 67 | 648 | 728 | 4.07e-21 | 86.7 |
| MsG0080048320.01.T02 | MTR_5g005600 | 54.930 | 71 | 23 | 1 | 6 | 67 | 624 | 694 | 5.91e-19 | 80.5 |
| MsG0080048320.01.T02 | MTR_2g101720 | 40.506 | 79 | 33 | 1 | 3 | 67 | 689 | 767 | 7.44e-14 | 65.9 |
| MsG0080048320.01.T02 | MTR_0198s0030 | 36.905 | 84 | 33 | 2 | 4 | 67 | 646 | 729 | 6.60e-12 | 60.5 |
| MsG0080048320.01.T03 | MTR_2g088470 | 76.923 | 65 | 5 | 1 | 1 | 55 | 646 | 710 | 5.11e-21 | 85.5 |
| MsG0080048320.01.T03 | MTR_4g047800 | 71.429 | 70 | 5 | 1 | 1 | 55 | 638 | 707 | 1.69e-20 | 84.3 |
| MsG0080048320.01.T03 | MTR_7g076080 | 64.615 | 65 | 11 | 1 | 3 | 55 | 688 | 752 | 1.27e-19 | 81.6 |
| MsG0080048320.01.T03 | MTR_8g432540 | 62.857 | 70 | 9 | 2 | 3 | 55 | 715 | 784 | 1.17e-18 | 79.0 |
| MsG0080048320.01.T03 | MTR_1g101280 | 57.143 | 70 | 13 | 1 | 3 | 55 | 659 | 728 | 3.80e-18 | 77.4 |
| MsG0080048320.01.T03 | MTR_5g005600 | 55.738 | 61 | 18 | 1 | 4 | 55 | 634 | 694 | 1.24e-14 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048320.01.T01 | AT1G05230 | 84.286 | 70 | 10 | 1 | 4 | 72 | 623 | 692 | 4.28e-34 | 123 |
| MsG0080048320.01.T01 | AT1G05230 | 84.286 | 70 | 10 | 1 | 4 | 72 | 625 | 694 | 4.29e-34 | 123 |
| MsG0080048320.01.T01 | AT1G05230 | 84.286 | 70 | 10 | 1 | 4 | 72 | 625 | 694 | 4.29e-34 | 123 |
| MsG0080048320.01.T01 | AT1G05230 | 84.286 | 70 | 10 | 1 | 4 | 72 | 625 | 694 | 4.29e-34 | 123 |
| MsG0080048320.01.T01 | AT1G05230 | 84.286 | 70 | 10 | 1 | 4 | 72 | 625 | 694 | 4.29e-34 | 123 |
| MsG0080048320.01.T01 | AT1G05230 | 84.286 | 70 | 10 | 1 | 4 | 72 | 625 | 694 | 4.29e-34 | 123 |
| MsG0080048320.01.T01 | AT1G05230 | 84.286 | 70 | 10 | 1 | 4 | 72 | 625 | 694 | 4.29e-34 | 123 |
| MsG0080048320.01.T01 | AT1G05230 | 84.286 | 70 | 10 | 1 | 4 | 72 | 625 | 694 | 4.29e-34 | 123 |
| MsG0080048320.01.T01 | AT1G05230 | 84.286 | 70 | 10 | 1 | 4 | 72 | 625 | 694 | 4.29e-34 | 123 |
| MsG0080048320.01.T01 | AT1G05230 | 84.286 | 70 | 10 | 1 | 4 | 72 | 625 | 694 | 4.29e-34 | 123 |
| MsG0080048320.01.T01 | AT1G05230 | 84.286 | 70 | 10 | 1 | 4 | 72 | 625 | 694 | 4.29e-34 | 123 |
| MsG0080048320.01.T01 | AT4G04890 | 71.250 | 80 | 10 | 1 | 6 | 72 | 634 | 713 | 1.28e-29 | 110 |
| MsG0080048320.01.T01 | AT4G04890 | 71.250 | 80 | 10 | 1 | 6 | 72 | 634 | 713 | 1.28e-29 | 110 |
| MsG0080048320.01.T01 | AT3G61150 | 61.446 | 83 | 18 | 1 | 4 | 72 | 699 | 781 | 1.92e-25 | 99.4 |
| MsG0080048320.01.T01 | AT4G21750 | 61.290 | 93 | 10 | 1 | 6 | 72 | 643 | 735 | 2.11e-24 | 96.3 |
| MsG0080048320.01.T01 | AT4G21750 | 61.290 | 93 | 10 | 1 | 6 | 72 | 643 | 735 | 2.11e-24 | 96.3 |
| MsG0080048320.01.T01 | AT4G21750 | 61.290 | 93 | 10 | 1 | 6 | 72 | 643 | 735 | 2.11e-24 | 96.3 |
| MsG0080048320.01.T01 | AT4G21750 | 61.290 | 93 | 10 | 1 | 6 | 72 | 643 | 735 | 2.11e-24 | 96.3 |
| MsG0080048320.01.T01 | AT2G32370 | 61.429 | 70 | 25 | 2 | 4 | 72 | 630 | 698 | 2.02e-23 | 93.6 |
| MsG0080048320.01.T01 | AT4G00730 | 67.105 | 76 | 18 | 1 | 4 | 72 | 701 | 776 | 3.45e-21 | 87.0 |
| MsG0080048320.01.T01 | AT5G52170 | 56.962 | 79 | 24 | 2 | 4 | 72 | 578 | 656 | 4.35e-21 | 86.7 |
| MsG0080048320.01.T01 | AT5G52170 | 56.962 | 79 | 24 | 2 | 4 | 72 | 578 | 656 | 4.35e-21 | 86.7 |
| MsG0080048320.01.T01 | AT5G52170 | 56.962 | 79 | 24 | 2 | 4 | 72 | 583 | 661 | 4.57e-21 | 86.7 |
| MsG0080048320.01.T01 | AT1G73360 | 45.570 | 79 | 33 | 1 | 4 | 72 | 613 | 691 | 4.35e-18 | 78.2 |
| MsG0080048320.01.T01 | AT1G17920 | 52.174 | 69 | 28 | 1 | 4 | 72 | 596 | 659 | 1.42e-17 | 76.6 |
| MsG0080048320.01.T01 | AT1G17920 | 52.174 | 69 | 28 | 1 | 4 | 72 | 596 | 659 | 1.42e-17 | 76.6 |
| MsG0080048320.01.T01 | AT1G79840 | 41.772 | 79 | 37 | 1 | 3 | 72 | 584 | 662 | 1.17e-14 | 68.6 |
| MsG0080048320.01.T01 | AT1G79840 | 41.772 | 79 | 37 | 1 | 3 | 72 | 584 | 662 | 1.17e-14 | 68.6 |
| MsG0080048320.01.T01 | AT1G79840 | 41.772 | 79 | 37 | 1 | 3 | 72 | 642 | 720 | 1.17e-14 | 68.6 |
| MsG0080048320.01.T01 | AT1G79840 | 41.772 | 79 | 37 | 1 | 3 | 72 | 671 | 749 | 1.18e-14 | 68.6 |
| MsG0080048320.01.T01 | AT4G25530 | 50.000 | 70 | 33 | 2 | 4 | 71 | 590 | 659 | 6.81e-13 | 63.5 |
| MsG0080048320.01.T01 | AT3G03260 | 47.541 | 61 | 29 | 2 | 4 | 63 | 603 | 661 | 5.89e-11 | 57.8 |
| MsG0080048320.01.T02 | AT1G05230 | 81.429 | 70 | 7 | 1 | 4 | 67 | 623 | 692 | 2.36e-31 | 115 |
| MsG0080048320.01.T02 | AT1G05230 | 81.429 | 70 | 7 | 1 | 4 | 67 | 625 | 694 | 2.36e-31 | 115 |
| MsG0080048320.01.T02 | AT1G05230 | 81.429 | 70 | 7 | 1 | 4 | 67 | 625 | 694 | 2.36e-31 | 115 |
| MsG0080048320.01.T02 | AT1G05230 | 81.429 | 70 | 7 | 1 | 4 | 67 | 625 | 694 | 2.36e-31 | 115 |
| MsG0080048320.01.T02 | AT1G05230 | 81.429 | 70 | 7 | 1 | 4 | 67 | 625 | 694 | 2.36e-31 | 115 |
| MsG0080048320.01.T02 | AT1G05230 | 81.429 | 70 | 7 | 1 | 4 | 67 | 625 | 694 | 2.36e-31 | 115 |
| MsG0080048320.01.T02 | AT1G05230 | 81.429 | 70 | 7 | 1 | 4 | 67 | 625 | 694 | 2.36e-31 | 115 |
| MsG0080048320.01.T02 | AT1G05230 | 81.429 | 70 | 7 | 1 | 4 | 67 | 625 | 694 | 2.36e-31 | 115 |
| MsG0080048320.01.T02 | AT1G05230 | 81.429 | 70 | 7 | 1 | 4 | 67 | 625 | 694 | 2.36e-31 | 115 |
| MsG0080048320.01.T02 | AT1G05230 | 81.429 | 70 | 7 | 1 | 4 | 67 | 625 | 694 | 2.36e-31 | 115 |
| MsG0080048320.01.T02 | AT1G05230 | 81.429 | 70 | 7 | 1 | 4 | 67 | 625 | 694 | 2.36e-31 | 115 |
| MsG0080048320.01.T02 | AT4G04890 | 70.000 | 80 | 6 | 1 | 6 | 67 | 634 | 713 | 1.01e-28 | 108 |
| MsG0080048320.01.T02 | AT4G04890 | 70.000 | 80 | 6 | 1 | 6 | 67 | 634 | 713 | 1.01e-28 | 108 |
| MsG0080048320.01.T02 | AT4G21750 | 58.065 | 93 | 8 | 1 | 6 | 67 | 643 | 735 | 1.10e-24 | 96.7 |
| MsG0080048320.01.T02 | AT4G21750 | 58.065 | 93 | 8 | 1 | 6 | 67 | 643 | 735 | 1.10e-24 | 96.7 |
| MsG0080048320.01.T02 | AT4G21750 | 58.065 | 93 | 8 | 1 | 6 | 67 | 643 | 735 | 1.10e-24 | 96.7 |
| MsG0080048320.01.T02 | AT4G21750 | 58.065 | 93 | 8 | 1 | 6 | 67 | 643 | 735 | 1.10e-24 | 96.7 |
| MsG0080048320.01.T02 | AT3G61150 | 59.036 | 83 | 15 | 1 | 4 | 67 | 699 | 781 | 1.22e-23 | 94.0 |
| MsG0080048320.01.T02 | AT2G32370 | 59.420 | 69 | 23 | 2 | 4 | 67 | 630 | 698 | 1.01e-21 | 88.2 |
| MsG0080048320.01.T02 | AT4G00730 | 63.158 | 76 | 16 | 1 | 4 | 67 | 701 | 776 | 1.19e-21 | 88.2 |
| MsG0080048320.01.T02 | AT5G52170 | 54.430 | 79 | 21 | 1 | 4 | 67 | 578 | 656 | 7.24e-21 | 85.9 |
| MsG0080048320.01.T02 | AT5G52170 | 54.430 | 79 | 21 | 1 | 4 | 67 | 578 | 656 | 7.24e-21 | 85.9 |
| MsG0080048320.01.T02 | AT5G52170 | 54.430 | 79 | 21 | 1 | 4 | 67 | 583 | 661 | 7.24e-21 | 85.9 |
| MsG0080048320.01.T02 | AT1G17920 | 54.688 | 64 | 29 | 0 | 4 | 67 | 596 | 659 | 6.67e-19 | 80.1 |
| MsG0080048320.01.T02 | AT1G17920 | 54.688 | 64 | 29 | 0 | 4 | 67 | 596 | 659 | 6.67e-19 | 80.1 |
| MsG0080048320.01.T02 | AT1G73360 | 44.304 | 79 | 29 | 2 | 4 | 67 | 613 | 691 | 2.24e-15 | 70.1 |
| MsG0080048320.01.T02 | AT1G79840 | 41.772 | 79 | 32 | 1 | 3 | 67 | 584 | 662 | 1.35e-14 | 68.2 |
| MsG0080048320.01.T02 | AT1G79840 | 41.772 | 79 | 32 | 1 | 3 | 67 | 584 | 662 | 1.35e-14 | 68.2 |
| MsG0080048320.01.T02 | AT1G79840 | 41.772 | 79 | 32 | 1 | 3 | 67 | 642 | 720 | 1.36e-14 | 68.2 |
| MsG0080048320.01.T02 | AT1G79840 | 41.772 | 79 | 32 | 1 | 3 | 67 | 671 | 749 | 1.36e-14 | 68.2 |
| MsG0080048320.01.T02 | AT4G25530 | 47.143 | 70 | 30 | 1 | 4 | 66 | 590 | 659 | 1.11e-12 | 62.4 |
| MsG0080048320.01.T02 | AT3G03260 | 49.153 | 59 | 26 | 2 | 4 | 58 | 603 | 661 | 1.90e-11 | 58.9 |
| MsG0080048320.01.T03 | AT1G05230 | 81.967 | 61 | 5 | 1 | 1 | 55 | 632 | 692 | 8.54e-27 | 102 |
| MsG0080048320.01.T03 | AT1G05230 | 81.967 | 61 | 5 | 1 | 1 | 55 | 634 | 694 | 8.54e-27 | 102 |
| MsG0080048320.01.T03 | AT1G05230 | 81.967 | 61 | 5 | 1 | 1 | 55 | 634 | 694 | 8.54e-27 | 102 |
| MsG0080048320.01.T03 | AT1G05230 | 81.967 | 61 | 5 | 1 | 1 | 55 | 634 | 694 | 8.54e-27 | 102 |
| MsG0080048320.01.T03 | AT1G05230 | 81.967 | 61 | 5 | 1 | 1 | 55 | 634 | 694 | 8.54e-27 | 102 |
| MsG0080048320.01.T03 | AT1G05230 | 81.967 | 61 | 5 | 1 | 1 | 55 | 634 | 694 | 8.54e-27 | 102 |
| MsG0080048320.01.T03 | AT1G05230 | 81.967 | 61 | 5 | 1 | 1 | 55 | 634 | 694 | 8.54e-27 | 102 |
| MsG0080048320.01.T03 | AT1G05230 | 81.967 | 61 | 5 | 1 | 1 | 55 | 634 | 694 | 8.54e-27 | 102 |
| MsG0080048320.01.T03 | AT1G05230 | 81.967 | 61 | 5 | 1 | 1 | 55 | 634 | 694 | 8.54e-27 | 102 |
| MsG0080048320.01.T03 | AT1G05230 | 81.967 | 61 | 5 | 1 | 1 | 55 | 634 | 694 | 8.54e-27 | 102 |
| MsG0080048320.01.T03 | AT1G05230 | 81.967 | 61 | 5 | 1 | 1 | 55 | 634 | 694 | 8.54e-27 | 102 |
| MsG0080048320.01.T03 | AT4G04890 | 67.123 | 73 | 6 | 1 | 1 | 55 | 641 | 713 | 1.36e-23 | 93.2 |
| MsG0080048320.01.T03 | AT4G04890 | 67.123 | 73 | 6 | 1 | 1 | 55 | 641 | 713 | 1.36e-23 | 93.2 |
| MsG0080048320.01.T03 | AT4G21750 | 55.814 | 86 | 7 | 1 | 1 | 55 | 650 | 735 | 1.61e-20 | 84.3 |
| MsG0080048320.01.T03 | AT4G21750 | 55.814 | 86 | 7 | 1 | 1 | 55 | 650 | 735 | 1.61e-20 | 84.3 |
| MsG0080048320.01.T03 | AT4G21750 | 55.814 | 86 | 7 | 1 | 1 | 55 | 650 | 735 | 1.61e-20 | 84.3 |
| MsG0080048320.01.T03 | AT4G21750 | 55.814 | 86 | 7 | 1 | 1 | 55 | 650 | 735 | 1.61e-20 | 84.3 |
| MsG0080048320.01.T03 | AT3G61150 | 59.722 | 72 | 10 | 2 | 3 | 55 | 710 | 781 | 7.23e-18 | 76.6 |
| MsG0080048320.01.T03 | AT2G32370 | 58.333 | 60 | 20 | 2 | 1 | 55 | 639 | 698 | 6.19e-17 | 73.9 |
| MsG0080048320.01.T03 | AT4G00730 | 63.077 | 65 | 12 | 1 | 3 | 55 | 712 | 776 | 7.34e-17 | 73.9 |
| MsG0080048320.01.T03 | AT5G52170 | 55.882 | 68 | 15 | 2 | 3 | 55 | 589 | 656 | 2.08e-15 | 69.7 |
| MsG0080048320.01.T03 | AT5G52170 | 55.882 | 68 | 15 | 2 | 3 | 55 | 589 | 656 | 2.08e-15 | 69.7 |
| MsG0080048320.01.T03 | AT5G52170 | 55.882 | 68 | 15 | 2 | 3 | 55 | 594 | 661 | 2.25e-15 | 69.7 |
| MsG0080048320.01.T03 | AT1G17920 | 54.717 | 53 | 24 | 0 | 3 | 55 | 607 | 659 | 1.48e-14 | 67.4 |
| MsG0080048320.01.T03 | AT1G17920 | 54.717 | 53 | 24 | 0 | 3 | 55 | 607 | 659 | 1.48e-14 | 67.4 |
| MsG0080048320.01.T03 | AT1G73360 | 45.714 | 70 | 23 | 2 | 1 | 55 | 622 | 691 | 7.98e-13 | 62.4 |
Find 23 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTATGAATGTAGTTCTAAA+TGG | 0.363093 | contig271end:+257864 | MsG0080048320.01.T02:CDS |
| GGAAGAAGTGCTACATAATC+TGG | 0.371226 | contig271end:-257894 | None:intergenic |
| TATCTATGCACCGGTAGATA+TGG | 0.387839 | contig271end:+257839 | MsG0080048320.01.T02:CDS |
| GCTATCCTCCCTGATGGAAA+TGG | 0.410049 | contig271end:+257927 | MsG0080048320.01.T02:intron |
| CTCCCTGATGGAAATGGAAA+TGG | 0.415022 | contig271end:+257933 | MsG0080048320.01.T02:intron |
| TATGTAGCACTTCTTCCATC+AGG | 0.428228 | contig271end:+257900 | MsG0080048320.01.T02:CDS |
| CTCCAACTGCTAAACTCTCT+TGG | 0.429852 | contig271end:+258048 | MsG0080048320.01.T02:CDS |
| ACTCCATTTCCATTTCCATC+AGG | 0.444605 | contig271end:-257936 | None:intergenic |
| GGAGGAGGAGGAGCTGGTGC+TGG | 0.452877 | contig271end:+257981 | MsG0080048320.01.T02:intron |
| GGAGGAGGAGCTGGTGCTGG+TGG | 0.482067 | contig271end:+257984 | MsG0080048320.01.T02:intron |
| CTCCATTTCCATTTCCATCA+GGG | 0.484372 | contig271end:-257935 | None:intergenic |
| GAGAGTGTAACAGATGCAAC+AGG | 0.516631 | contig271end:+257809 | None:intergenic |
| TTTGAAAAGCGACGGTCAGA+AGG | 0.541068 | contig271end:-258010 | None:intergenic |
| TTCATAGCAACCATATCTAC+CGG | 0.568044 | contig271end:-257849 | None:intergenic |
| ATCCAAGAGAGTTTAGCAGT+TGG | 0.571937 | contig271end:-258050 | None:intergenic |
| GGGAGGATAGCAAATCCTGA+TGG | 0.577076 | contig271end:-257915 | None:intergenic |
| GGCCATTGTTATCTATGCAC+CGG | 0.582917 | contig271end:+257830 | MsG0080048320.01.T02:CDS |
| GGATTTGCTATCCTCCCTGA+TGG | 0.589528 | contig271end:+257921 | MsG0080048320.01.T02:CDS |
| AACCAAGATTTGAAAAGCGA+CGG | 0.590397 | contig271end:-258018 | None:intergenic |
| TTGAAAAGCGACGGTCAGAA+GGG | 0.614971 | contig271end:-258009 | None:intergenic |
| ATGAATGTAGTTCTAAATGG+AGG | 0.639925 | contig271end:+257867 | MsG0080048320.01.T02:CDS |
| CATTTCCATTTCCATCAGGG+AGG | 0.666915 | contig271end:-257932 | None:intergenic |
| TACCGGTGCATAGATAACAA+TGG | 0.698020 | contig271end:-257832 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGAATGTAGTTCTAAATGG+AGG | + | contig271end:257867-257886 | MsG0080048320.01.T02:CDS | 30.0% |
| !! | GCTATGAATGTAGTTCTAAA+TGG | + | contig271end:257864-257883 | MsG0080048320.01.T02:CDS | 30.0% |
| AACCAAGATTTGAAAAGCGA+CGG | - | contig271end:258021-258040 | None:intergenic | 35.0% | |
| TTCATAGCAACCATATCTAC+CGG | - | contig271end:257852-257871 | None:intergenic | 35.0% | |
| ! | AGAGAATCAAAGCTTTTGTC+TGG | + | contig271end:258109-258128 | MsG0080048320.01.T02:CDS | 35.0% |
| ACTCCATTTCCATTTCCATC+AGG | - | contig271end:257939-257958 | None:intergenic | 40.0% | |
| ATCCAAGAGAGTTTAGCAGT+TGG | - | contig271end:258053-258072 | None:intergenic | 40.0% | |
| CTCCATTTCCATTTCCATCA+GGG | - | contig271end:257938-257957 | None:intergenic | 40.0% | |
| GATGGAAATGGAAATGGAGT+CGG | + | contig271end:257939-257958 | MsG0080048320.01.T02:intron | 40.0% | |
| TACCGGTGCATAGATAACAA+TGG | - | contig271end:257835-257854 | None:intergenic | 40.0% | |
| TATGTAGCACTTCTTCCATC+AGG | + | contig271end:257900-257919 | MsG0080048320.01.T02:CDS | 40.0% | |
| ! | TATCTATGCACCGGTAGATA+TGG | + | contig271end:257839-257858 | MsG0080048320.01.T02:CDS | 40.0% |
| !! | GGAAGAAGTGCTACATAATC+TGG | - | contig271end:257897-257916 | None:intergenic | 40.0% |
| CATTTCCATTTCCATCAGGG+AGG | - | contig271end:257935-257954 | None:intergenic | 45.0% | |
| CTCCAACTGCTAAACTCTCT+TGG | + | contig271end:258048-258067 | MsG0080048320.01.T02:CDS | 45.0% | |
| CTCCCTGATGGAAATGGAAA+TGG | + | contig271end:257933-257952 | MsG0080048320.01.T02:intron | 45.0% | |
| GGCCATTGTTATCTATGCAC+CGG | + | contig271end:257830-257849 | MsG0080048320.01.T02:CDS | 45.0% | |
| TTGAAAAGCGACGGTCAGAA+GGG | - | contig271end:258012-258031 | None:intergenic | 45.0% | |
| TTTGAAAAGCGACGGTCAGA+AGG | - | contig271end:258013-258032 | None:intergenic | 45.0% | |
| !!! | GACCGTCGCTTTTCAAATCT+TGG | + | contig271end:258016-258035 | MsG0080048320.01.T02:CDS | 45.0% |
| GAAATGGAAATGGAGTCGGC+GGG | + | contig271end:257943-257962 | MsG0080048320.01.T02:intron | 50.0% | |
| GCTATCCTCCCTGATGGAAA+TGG | + | contig271end:257927-257946 | MsG0080048320.01.T02:intron | 50.0% | |
| GGAAATGGAAATGGAGTCGG+CGG | + | contig271end:257942-257961 | MsG0080048320.01.T02:intron | 50.0% | |
| GGGAGGATAGCAAATCCTGA+TGG | - | contig271end:257918-257937 | None:intergenic | 50.0% | |
| !! | GGATTTGCTATCCTCCCTGA+TGG | + | contig271end:257921-257940 | MsG0080048320.01.T02:CDS | 50.0% |
| TGTGAAACTGGCGGAGGAGG+AGG | + | contig271end:257966-257985 | MsG0080048320.01.T02:intron | 60.0% | |
| GAAACTGGCGGAGGAGGAGG+AGG | + | contig271end:257969-257988 | MsG0080048320.01.T02:intron | 65.0% | |
| GGAGTCGGCGGGTGTGAAAC+TGG | + | contig271end:257954-257973 | MsG0080048320.01.T02:intron | 65.0% | |
| GGGTGTGAAACTGGCGGAGG+AGG | + | contig271end:257963-257982 | MsG0080048320.01.T02:intron | 65.0% | |
| GTCGGCGGGTGTGAAACTGG+CGG | + | contig271end:257957-257976 | MsG0080048320.01.T02:intron | 65.0% | |
| GGAGGAGGAGGAGCTGGTGC+TGG | + | contig271end:257981-258000 | MsG0080048320.01.T02:intron | 70.0% | |
| GGCGGGTGTGAAACTGGCGG+AGG | + | contig271end:257960-257979 | MsG0080048320.01.T02:intron | 70.0% | |
| ! | GGAGGAGGAGCTGGTGCTGG+TGG | + | contig271end:257984-258003 | MsG0080048320.01.T02:intron | 70.0% |
| GGCGGAGGAGGAGGAGGAGC+TGG | + | contig271end:257975-257994 | MsG0080048320.01.T02:intron | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig271end | gene | 257822 | 258160 | 257822 | ID=MsG0080048320.01;Name=MsG0080048320.01 |
| contig271end | mRNA | 257822 | 258160 | 257822 | ID=MsG0080048320.01.T01;Parent=MsG0080048320.01;Name=MsG0080048320.01.T01;_AED=0.47;_eAED=0.56;_QI=0|0|0|1|0|0|2|0|102 |
| contig271end | exon | 257985 | 258160 | 257985 | ID=MsG0080048320.01.T01:exon:19250;Parent=MsG0080048320.01.T01 |
| contig271end | exon | 257822 | 257954 | 257822 | ID=MsG0080048320.01.T01:exon:19249;Parent=MsG0080048320.01.T01 |
| contig271end | CDS | 257822 | 257954 | 257822 | ID=MsG0080048320.01.T01:cds;Parent=MsG0080048320.01.T01 |
| contig271end | CDS | 257985 | 258160 | 257985 | ID=MsG0080048320.01.T01:cds;Parent=MsG0080048320.01.T01 |
| contig271end | mRNA | 257822 | 258160 | 257822 | ID=MsG0080048320.01.T02;Parent=MsG0080048320.01;Name=MsG0080048320.01.T02;_AED=0.47;_eAED=0.56;_QI=0|0|0|1|0|0|2|0|97 |
| contig271end | exon | 257988 | 258160 | 257988 | ID=MsG0080048320.01.T02:exon:19252;Parent=MsG0080048320.01.T02 |
| contig271end | exon | 257822 | 257942 | 257822 | ID=MsG0080048320.01.T02:exon:19251;Parent=MsG0080048320.01.T02 |
| contig271end | CDS | 257822 | 257942 | 257822 | ID=MsG0080048320.01.T02:cds;Parent=MsG0080048320.01.T02 |
| contig271end | CDS | 257988 | 258160 | 257988 | ID=MsG0080048320.01.T02:cds;Parent=MsG0080048320.01.T02 |
| contig271end | mRNA | 257858 | 258160 | 257858 | ID=MsG0080048320.01.T03;Parent=MsG0080048320.01;Name=MsG0080048320.01.T03;_AED=0.47;_eAED=0.58;_QI=0|0|0|1|0|0|2|0|85 |
| contig271end | exon | 258003 | 258160 | 258003 | ID=MsG0080048320.01.T03:exon:19254;Parent=MsG0080048320.01.T03 |
| contig271end | exon | 257858 | 257957 | 257858 | ID=MsG0080048320.01.T03:exon:19253;Parent=MsG0080048320.01.T03 |
| contig271end | CDS | 257858 | 257957 | 257858 | ID=MsG0080048320.01.T03:cds;Parent=MsG0080048320.01.T03 |
| contig271end | CDS | 258003 | 258160 | 258003 | ID=MsG0080048320.01.T03:cds;Parent=MsG0080048320.01.T03 |
| Gene Sequence |
| Protein sequence |
