AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003741.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003741.01.T01 MTR_1g069085 95.918 49 2 0 1 49 1 49 1.57e-27 98.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 54 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGGTGGTGGTGGACTTCC+TGG 0.178208 1:+67523718 MsG0180003741.01.T01:CDS
CTTGGTGGTGCTGGTGGCTT+TGG 0.225781 1:+67523742 MsG0180003741.01.T01:CDS
GGTGATGCTGGTGGTGGACT+TGG 0.309365 1:+67523652 MsG0180003741.01.T01:CDS
CTCCCCGGACTTGGCGGTGC+TGG 0.314858 1:+67523769 MsG0180003741.01.T01:CDS
GGTAACAATGGACTGCCTTT+TGG 0.332715 1:+67523607 MsG0180003741.01.T01:CDS
TTTGGTGGTGCTGGTGATGC+TGG 0.337285 1:+67523640 MsG0180003741.01.T01:CDS
CTTGGCGGTGCTGGTGGACT+CGG 0.338493 1:+67523778 MsG0180003741.01.T01:CDS
CTTCCTGGACTTGGTGGTGC+TGG 0.351425 1:+67523733 MsG0180003741.01.T01:CDS
TTTGGTGGACTCCCCGGACT+TGG 0.357923 1:+67523760 MsG0180003741.01.T01:CDS
TCCACCAGCACCGCCAAGTC+CGG 0.368793 1:-67523773 None:intergenic
GGTGTAGGCTTTGGTGGTGC+TGG 0.371956 1:+67523631 MsG0180003741.01.T01:CDS
GCTGGTGGACTCGGAGGACT+TGG 0.376726 1:+67523787 MsG0180003741.01.T01:CDS
GGTGGTGGACTTCCTGGACT+TGG 0.376973 1:+67523724 MsG0180003741.01.T01:CDS
GGAATCGGTGGTGGTGGTGG+TGG 0.379428 1:+67523706 MsG0180003741.01.T01:CDS
GGTGGCGGTGCTGGTGGACT+CGG 0.386691 1:+67523682 MsG0180003741.01.T01:CDS
CTCGGAGGACTTGGTGGTAC+TGG 0.399755 1:+67523796 MsG0180003741.01.T01:CDS
GGTGCTGGTGATGCTGGTGG+TGG 0.408529 1:+67523646 MsG0180003741.01.T01:CDS
GCTGGTGGACTCGGAGGAAT+CGG 0.409280 1:+67523691 MsG0180003741.01.T01:CDS
GGTGGTGGACTTGGAGGACT+AGG 0.420340 1:+67523661 MsG0180003741.01.T01:CDS
GGTGGTGCTGGTGGCTTTGG+TGG 0.420680 1:+67523745 MsG0180003741.01.T01:CDS
CCACCAGCACCGCCAAGTCC+GGG 0.427720 1:-67523772 None:intergenic
GGAGGAATCGGTGGTGGTGG+TGG 0.434584 1:+67523703 MsG0180003741.01.T01:CDS
CTCGGAGGAATCGGTGGTGG+TGG 0.446365 1:+67523700 MsG0180003741.01.T01:CDS
GGAGGACTAGGTGGCGGTGC+TGG 0.453242 1:+67523673 MsG0180003741.01.T01:CDS
CCCGGACTTGGCGGTGCTGG+TGG 0.458285 1:+67523772 MsG0180003741.01.T01:CDS
TTTGGAGGTGTAGGCTTTGG+TGG 0.458483 1:+67523625 MsG0180003741.01.T01:CDS
AATGTCTAAGTGGTGCATTT+TGG 0.458799 1:+67523471 None:intergenic
GATGCTGGTGGTGGACTTGG+AGG 0.463937 1:+67523655 MsG0180003741.01.T01:CDS
GGTGGACTCCCCGGACTTGG+CGG 0.480200 1:+67523763 MsG0180003741.01.T01:CDS
GGTGGACTTCCTGGACTTGG+TGG 0.484979 1:+67523727 MsG0180003741.01.T01:CDS
GGTGGCTTTGGTGGACTCCC+CGG 0.490321 1:+67523754 MsG0180003741.01.T01:CDS
GGACTCGGAGGAATCGGTGG+TGG 0.495212 1:+67523697 MsG0180003741.01.T01:CDS
AAGAATTACATGACTGGAGC+TGG 0.500445 1:+67523568 MsG0180003741.01.T01:CDS
GGACTAGGTGGCGGTGCTGG+TGG 0.509217 1:+67523676 MsG0180003741.01.T01:CDS
GGACTTGGTGGTACTGGTGG+TGG 0.520354 1:+67523802 MsG0180003741.01.T01:CDS
TGCTGGAGAAGATGGTTTGA+AGG 0.527688 1:+67523540 MsG0180003741.01.T01:CDS
TTCAAACCATCTTCTCCAGC+AGG 0.528729 1:-67523538 None:intergenic
CCTGGACTTGGTGGTGCTGG+TGG 0.528901 1:+67523736 MsG0180003741.01.T01:CDS
TAACTTGACACTCAAGGTTG+AGG 0.530118 1:-67523832 None:intergenic
CCACCAGCACCACCAAGTCC+AGG 0.542661 1:-67523736 None:intergenic
CCAAAGCCTACACCTCCAAA+AGG 0.544740 1:-67523622 None:intergenic
GATCAGAAGAATTACATGAC+TGG 0.553149 1:+67523562 MsG0180003741.01.T01:CDS
GGTGGTGCTGGTGATGCTGG+TGG 0.553318 1:+67523643 MsG0180003741.01.T01:CDS
AATGCAAGAAATGTGCCTGC+TGG 0.560154 1:+67523523 MsG0180003741.01.T01:CDS
GGTGGACTCGGAGGACTTGG+TGG 0.562731 1:+67523790 MsG0180003741.01.T01:CDS
AATGTGCCTGCTGGAGAAGA+TGG 0.564431 1:+67523532 MsG0180003741.01.T01:CDS
GGTGGACTCGGAGGAATCGG+TGG 0.565750 1:+67523694 MsG0180003741.01.T01:CDS
CACCAGCACCGCCAAGTCCG+GGG 0.580869 1:-67523771 None:intergenic
GGCGGTGCTGGTGGACTCGG+AGG 0.589973 1:+67523685 MsG0180003741.01.T01:CDS
GGAGGACTTGGTGGTACTGG+TGG 0.611733 1:+67523799 MsG0180003741.01.T01:CDS
GGCGGTGCTGGTGGACTCGG+AGG 0.621136 1:+67523781 MsG0180003741.01.T01:CDS
GGTGGACTTGGAGGACTAGG+TGG 0.622805 1:+67523664 MsG0180003741.01.T01:CDS
GGACTTGGAGGACTAGGTGG+CGG 0.646664 1:+67523667 MsG0180003741.01.T01:CDS
TGAAGAGAGAAATGTCTAAG+TGG 0.655500 1:+67523461 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GATCAGAAGAATTACATGAC+TGG + Chr1:67523562-67523581 MsG0180003741.01.T01:CDS 35.0%
!!! TTTTCAGGCATTGGTAACAA+TGG + Chr1:67523595-67523614 MsG0180003741.01.T01:CDS 35.0%
AAGAATTACATGACTGGAGC+TGG + Chr1:67523568-67523587 MsG0180003741.01.T01:CDS 40.0%
!!! GTGGTGCATTTTGGTTGTTT+TGG + Chr1:67523480-67523499 MsG0180003741.01.T01:CDS 40.0%
AATGCAAGAAATGTGCCTGC+TGG + Chr1:67523523-67523542 MsG0180003741.01.T01:CDS 45.0%
TGCTGGAGAAGATGGTTTGA+AGG + Chr1:67523540-67523559 MsG0180003741.01.T01:CDS 45.0%
TTCAAACCATCTTCTCCAGC+AGG - Chr1:67523541-67523560 None:intergenic 45.0%
!!! AACAATGGACTGCCTTTTGG+AGG + Chr1:67523610-67523629 MsG0180003741.01.T01:CDS 45.0%
!!! ACTGGAGCTGGTGTTTTTTC+AGG + Chr1:67523580-67523599 MsG0180003741.01.T01:CDS 45.0%
!!! GCTGGTGTTTTTTCAGGCAT+TGG + Chr1:67523586-67523605 MsG0180003741.01.T01:CDS 45.0%
!!! GGTAACAATGGACTGCCTTT+TGG + Chr1:67523607-67523626 MsG0180003741.01.T01:CDS 45.0%
AATGTGCCTGCTGGAGAAGA+TGG + Chr1:67523532-67523551 MsG0180003741.01.T01:CDS 50.0%
CCAAAGCCTACACCTCCAAA+AGG - Chr1:67523625-67523644 None:intergenic 50.0%
! TTTGGAGGTGTAGGCTTTGG+TGG + Chr1:67523625-67523644 MsG0180003741.01.T01:CDS 50.0%
!! CCTTTTGGAGGTGTAGGCTT+TGG + Chr1:67523622-67523641 MsG0180003741.01.T01:CDS 50.0%
!!! GGTTGTTTTGGCTCTTGCTG+TGG + Chr1:67523492-67523511 MsG0180003741.01.T01:CDS 50.0%
!! GGACTGCCTTTTGGAGGTGT+AGG + Chr1:67523616-67523635 MsG0180003741.01.T01:CDS 55.0%
!! TTTGGTGGTGCTGGTGATGC+TGG + Chr1:67523640-67523659 MsG0180003741.01.T01:CDS 55.0%
GGACTTGGAGGACTAGGTGG+CGG + Chr1:67523667-67523686 MsG0180003741.01.T01:CDS 60.0%
! GGTGGACTTCCTGGACTTGG+TGG + Chr1:67523727-67523746 MsG0180003741.01.T01:CDS 60.0%
! GGTGGACTTGGAGGACTAGG+TGG + Chr1:67523664-67523683 MsG0180003741.01.T01:CDS 60.0%
!! CTCGGAGGACTTGGTGGTAC+TGG + Chr1:67523796-67523815 MsG0180003741.01.T01:CDS 60.0%
!! CTTCCTGGACTTGGTGGTGC+TGG + Chr1:67523733-67523752 MsG0180003741.01.T01:CDS 60.0%
!! CTTGGTGGTGCTGGTGGCTT+TGG + Chr1:67523742-67523761 MsG0180003741.01.T01:CDS 60.0%
!! GATGCTGGTGGTGGACTTGG+AGG + Chr1:67523655-67523674 MsG0180003741.01.T01:CDS 60.0%
!! GCTGGTGGACTCGGAGGAAT+CGG + Chr1:67523691-67523710 MsG0180003741.01.T01:CDS 60.0%
!! GGACTTGGTGGTACTGGTGG+TGG + Chr1:67523802-67523821 MsG0180003741.01.T01:CDS 60.0%
!! GGAGGACTTGGTGGTACTGG+TGG + Chr1:67523799-67523818 MsG0180003741.01.T01:CDS 60.0%
!! GGTGATGCTGGTGGTGGACT+TGG + Chr1:67523652-67523671 MsG0180003741.01.T01:CDS 60.0%
!! GGTGGTGGACTTCCTGGACT+TGG + Chr1:67523724-67523743 MsG0180003741.01.T01:CDS 60.0%
!! GGTGGTGGACTTGGAGGACT+AGG + Chr1:67523661-67523680 MsG0180003741.01.T01:CDS 60.0%
!! GGTGTAGGCTTTGGTGGTGC+TGG + Chr1:67523631-67523650 MsG0180003741.01.T01:CDS 60.0%
!! TTTGGTGGACTCCCCGGACT+TGG + Chr1:67523760-67523779 MsG0180003741.01.T01:CDS 60.0%
CCACCAGCACCACCAAGTCC+AGG - Chr1:67523739-67523758 None:intergenic 65.0%
GGACTCGGAGGAATCGGTGG+TGG + Chr1:67523697-67523716 MsG0180003741.01.T01:CDS 65.0%
TCCACCAGCACCGCCAAGTC+CGG - Chr1:67523776-67523795 None:intergenic 65.0%
! CTCGGAGGAATCGGTGGTGG+TGG + Chr1:67523700-67523719 MsG0180003741.01.T01:CDS 65.0%
! GGTGGACTCGGAGGAATCGG+TGG + Chr1:67523694-67523713 MsG0180003741.01.T01:CDS 65.0%
! GGTGGACTCGGAGGACTTGG+TGG + Chr1:67523790-67523809 MsG0180003741.01.T01:CDS 65.0%
!! ATCGGTGGTGGTGGTGGTGG+TGG + Chr1:67523709-67523728 MsG0180003741.01.T01:CDS 65.0%
!! CCTGGACTTGGTGGTGCTGG+TGG + Chr1:67523736-67523755 MsG0180003741.01.T01:CDS 65.0%
!! CTTGGCGGTGCTGGTGGACT+CGG + Chr1:67523778-67523797 MsG0180003741.01.T01:CDS 65.0%
!! GCTGGTGGACTCGGAGGACT+TGG + Chr1:67523787-67523806 MsG0180003741.01.T01:CDS 65.0%
!! GGAATCGGTGGTGGTGGTGG+TGG + Chr1:67523706-67523725 MsG0180003741.01.T01:CDS 65.0%
!! GGAGGAATCGGTGGTGGTGG+TGG + Chr1:67523703-67523722 MsG0180003741.01.T01:CDS 65.0%
!! GGTGCTGGTGATGCTGGTGG+TGG + Chr1:67523646-67523665 MsG0180003741.01.T01:CDS 65.0%
!! GGTGGCTTTGGTGGACTCCC+CGG + Chr1:67523754-67523773 MsG0180003741.01.T01:CDS 65.0%
!! GGTGGTGCTGGTGATGCTGG+TGG + Chr1:67523643-67523662 MsG0180003741.01.T01:CDS 65.0%
!! GGTGGTGCTGGTGGCTTTGG+TGG + Chr1:67523745-67523764 MsG0180003741.01.T01:CDS 65.0%
!! GGTGGTGGTGGTGGACTTCC+TGG + Chr1:67523718-67523737 MsG0180003741.01.T01:CDS 65.0%
CACCAGCACCGCCAAGTCCG+GGG - Chr1:67523774-67523793 None:intergenic 70.0%
CCACCAGCACCGCCAAGTCC+GGG - Chr1:67523775-67523794 None:intergenic 70.0%
! GGACTAGGTGGCGGTGCTGG+TGG + Chr1:67523676-67523695 MsG0180003741.01.T01:CDS 70.0%
! GGAGGACTAGGTGGCGGTGC+TGG + Chr1:67523673-67523692 MsG0180003741.01.T01:CDS 70.0%
! GGTGGACTCCCCGGACTTGG+CGG + Chr1:67523763-67523782 MsG0180003741.01.T01:CDS 70.0%
!! GGTGGCGGTGCTGGTGGACT+CGG + Chr1:67523682-67523701 MsG0180003741.01.T01:CDS 70.0%
CTCCCCGGACTTGGCGGTGC+TGG + Chr1:67523769-67523788 MsG0180003741.01.T01:CDS 75.0%
! CCCGGACTTGGCGGTGCTGG+TGG + Chr1:67523772-67523791 MsG0180003741.01.T01:CDS 75.0%
!! GGCGGTGCTGGTGGACTCGG+AGG + Chr1:67523685-67523704 MsG0180003741.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
Chr1 gene 67523472 67523843 67523472 ID=MsG0180003741.01;Name=MsG0180003741.01
Chr1 mRNA 67523472 67523843 67523472 ID=MsG0180003741.01.T01;Parent=MsG0180003741.01;Name=MsG0180003741.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|123
Chr1 exon 67523472 67523843 67523472 ID=MsG0180003741.01.T01:exon:4027;Parent=MsG0180003741.01.T01
Chr1 CDS 67523472 67523843 67523472 ID=MsG0180003741.01.T01:cds;Parent=MsG0180003741.01.T01
Gene Sequence

>MsG0180003741.01.T01

ATGTCTAAGTGGTGCATTTTGGTTGTTTTGGCTCTTGCTGTGGTTGCAACAAATGCAAGAAATGTGCCTGCTGGAGAAGATGGTTTGAAGGATCAGAAGAATTACATGACTGGAGCTGGTGTTTTTTCAGGCATTGGTAACAATGGACTGCCTTTTGGAGGTGTAGGCTTTGGTGGTGCTGGTGATGCTGGTGGTGGACTTGGAGGACTAGGTGGCGGTGCTGGTGGACTCGGAGGAATCGGTGGTGGTGGTGGTGGTGGACTTCCTGGACTTGGTGGTGCTGGTGGCTTTGGTGGACTCCCCGGACTTGGCGGTGCTGGTGGACTCGGAGGACTTGGTGGTACTGGTGGTGGTGTCTTTCCTCAACCTTGA

Protein sequence

>MsG0180003741.01.T01

MSKWCILVVLALAVVATNARNVPAGEDGLKDQKNYMTGAGVFSGIGNNGLPFGGVGFGGAGDAGGGLGGLGGGAGGLGGIGGGGGGGLPGLGGAGGFGGLPGLGGAGGLGGLGGTGGGVFPQP*