AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041259.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041259.01.T01 MTR_7g108130 95.812 191 7 1 1 190 1 191 6.31e-132 368
MsG0780041259.01.T01 MTR_4g118810 42.938 177 90 2 1 166 1 177 1.49e-44 147
MsG0780041259.01.T01 MTR_2g020060 44.242 165 85 2 31 189 26 189 9.17e-39 131
MsG0780041259.01.T01 MTR_4g118800 34.783 161 95 4 26 181 26 181 1.94e-22 89.7
MsG0780041259.01.T01 MTR_8g089300 30.409 171 109 4 2 166 4 170 4.12e-20 83.6
MsG0780041259.01.T01 MTR_4g081880 29.560 159 107 3 21 177 22 177 2.81e-17 76.3
MsG0780041259.01.T01 MTR_0097s0070 29.452 146 97 2 45 184 44 189 1.17e-15 72.0
MsG0780041259.01.T01 MTR_5g041900 28.571 154 105 3 26 177 26 176 6.84e-15 69.7
MsG0780041259.01.T01 MTR_0004s0210 27.950 161 106 3 21 181 1 151 1.75e-14 68.2
MsG0780041259.01.T01 MTR_7g011090 27.500 160 111 3 20 177 20 176 1.27e-13 66.2
MsG0780041259.01.T01 MTR_7g083120 29.078 141 94 3 31 169 33 169 2.41e-13 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041259.01.T01 AT4G15630 39.267 191 109 2 1 185 1 190 2.43e-42 140
MsG0780041259.01.T01 AT4G15620 37.895 190 111 2 1 184 1 189 3.85e-39 132
MsG0780041259.01.T01 AT4G15610 30.994 171 114 3 1 167 1 171 4.36e-23 91.7
MsG0780041259.01.T01 AT3G06390 34.074 135 85 2 29 159 38 172 1.31e-17 77.4
MsG0780041259.01.T01 AT5G44550 33.125 160 99 3 32 184 22 180 6.64e-17 75.5
MsG0780041259.01.T01 AT4G15610 31.532 111 70 2 63 167 5 115 2.67e-16 72.4
MsG0780041259.01.T01 AT4G20390 36.090 133 79 3 32 158 22 154 1.06e-15 72.0
MsG0780041259.01.T01 AT5G15290 27.211 147 100 3 21 164 21 163 3.99e-15 70.5
MsG0780041259.01.T01 AT1G14160 27.607 163 115 3 26 187 49 209 2.75e-12 63.2
MsG0780041259.01.T01 AT4G16442 27.273 176 115 4 17 185 2 171 2.19e-11 60.5

Find 32 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTACTATCATTGTGGGTTT+TGG 0.265618 7:+88885851 MsG0780041259.01.T01:CDS
GATGTGTTGCTTAGGGTTCT+TGG 0.291243 7:+88885809 MsG0780041259.01.T01:CDS
GAGAGTGAGCCACAAGTAAA+AGG 0.319910 7:-88887916 None:intergenic
GCTTTGGTTCTATCTATCAT+TGG 0.323071 7:+88887881 MsG0780041259.01.T01:CDS
TTCATATGCTGCAATTTCTA+TGG 0.345306 7:+88887655 MsG0780041259.01.T01:CDS
CACTGCACCAGCAGCTCCAT+TGG 0.363063 7:-88887771 None:intergenic
CATCCACACTTGTCTTTGAC+TGG 0.380763 7:-88885733 None:intergenic
TCTAGTTTGATCTATAATAA+TGG 0.389846 7:-88887949 None:intergenic
AATGCTAGACCTAGTTATAA+TGG 0.390891 7:+88887733 MsG0780041259.01.T01:CDS
GTGTTTATGATGTGTTGCTT+AGG 0.399152 7:+88885801 MsG0780041259.01.T01:CDS
CTCACTGCTACTATCATTGT+GGG 0.403424 7:+88885845 MsG0780041259.01.T01:CDS
GCTGGTGCAGTGGGAGTGTT+AGG 0.413062 7:+88887782 MsG0780041259.01.T01:CDS
TTCTCAGCCAATGGAGCTGC+TGG 0.424568 7:+88887764 MsG0780041259.01.T01:CDS
TCACAGCGATGTGAAGGTTA+TGG 0.427097 7:+88885760 MsG0780041259.01.T01:CDS
AACAGTCATGTGACGACAAT+AGG 0.431776 7:-88887858 None:intergenic
TGTTTATGATGTGTTGCTTA+GGG 0.433057 7:+88885802 MsG0780041259.01.T01:CDS
TTCTTGGTGACAAATGCTAT+AGG 0.474689 7:+88887629 MsG0780041259.01.T01:CDS
TCGTCACATGACTGTTGCTT+TGG 0.482039 7:+88887865 MsG0780041259.01.T01:CDS
TCTCACTGCTACTATCATTG+TGG 0.489600 7:+88885844 MsG0780041259.01.T01:CDS
AAGGAAACTCGCACGTGCAA+TGG 0.499701 7:+88887810 MsG0780041259.01.T01:CDS
CAGTCAAAGACAAGTGTGGA+TGG 0.535899 7:+88885734 MsG0780041259.01.T01:CDS
GCATTACTCTTCTCAGCCAA+TGG 0.539824 7:+88887755 MsG0780041259.01.T01:CDS
AATGGAGCTGCTGGTGCAGT+GGG 0.549722 7:+88887773 MsG0780041259.01.T01:CDS
ATTCAAAGCTACTGCTAAGT+GGG 0.553173 7:+88885910 MsG0780041259.01.T01:CDS
CAATGGAGCTGCTGGTGCAG+TGG 0.564486 7:+88887772 MsG0780041259.01.T01:CDS
AATTCAAAGCTACTGCTAAG+TGG 0.570996 7:+88885909 MsG0780041259.01.T01:CDS
GTGGGAGTGTTAGGACAGAA+AGG 0.578933 7:+88887791 MsG0780041259.01.T01:CDS
TCAACAATTGCAAGAACCAG+TGG 0.593273 7:+88887683 MsG0780041259.01.T01:CDS
TGGAGTTCACAGCGATGTGA+AGG 0.594175 7:+88885754 MsG0780041259.01.T01:CDS
AGAGTAATGCCATTATAACT+AGG 0.597714 7:-88887742 None:intergenic
AAACCAGTCAAAGACAAGTG+TGG 0.653780 7:+88885730 MsG0780041259.01.T01:CDS
CACAAGTAAAAGGAAGACAG+TGG 0.729118 7:-88887906 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTTACTAAAAATAAAATAA+AGG - Chr7:88886440-88886459 None:intergenic 10.0%
!! TATAATATGTTAGTTAATAA+CGG + Chr7:88886459-88886478 MsG0780041259.01.T01:intron 10.0%
!! TTTAAAAAATTGTAAAAGAA+AGG - Chr7:88886184-88886203 None:intergenic 10.0%
!!! TTTTTCTTCAAAAAAATTAA+TGG - Chr7:88887222-88887241 None:intergenic 10.0%
!! AAAAAAATATAAAACAAACG+AGG - Chr7:88887559-88887578 None:intergenic 15.0%
!! AATACATTTATATTCCAAAT+AGG - Chr7:88887371-88887390 None:intergenic 15.0%
!! ATACATTTATATTCCAAATA+GGG - Chr7:88887370-88887389 None:intergenic 15.0%
!! CAAAAAAATAAAAACACTAT+AGG - Chr7:88886415-88886434 None:intergenic 15.0%
!! TTCATTAACAAATGTTATAA+GGG + Chr7:88886123-88886142 MsG0780041259.01.T01:intron 15.0%
!! TTTCATTAACAAATGTTATA+AGG + Chr7:88886122-88886141 MsG0780041259.01.T01:intron 15.0%
!!! AAATGTTTTAAGAAAGTAAA+TGG + Chr7:88886369-88886388 MsG0780041259.01.T01:intron 15.0%
!!! ATATATTGATTGATTACTAA+TGG - Chr7:88886528-88886547 None:intergenic 15.0%
!!! ATTTAGTTTTTGAAATGTTA+AGG + Chr7:88885998-88886017 MsG0780041259.01.T01:intron 15.0%
!!! GTATATAGTTTTGAAAATTT+AGG - Chr7:88886346-88886365 None:intergenic 15.0%
!!! TAAATAAACTGATTTATGTT+TGG - Chr7:88887027-88887046 None:intergenic 15.0%
!!! TATTTGGAATATAAATGTAT+TGG + Chr7:88887370-88887389 MsG0780041259.01.T01:intron 15.0%
!!! TTTAGTTTTTGAAATGTTAA+GGG + Chr7:88885999-88886018 MsG0780041259.01.T01:intron 15.0%
!!! TTTTTTTTTGAAAATGTTTC+TGG + Chr7:88886244-88886263 MsG0780041259.01.T01:intron 15.0%
!! AAAAAATTGTAAAAGAAAGG+TGG - Chr7:88886181-88886200 None:intergenic 20.0%
!! AAAGAAGGATAATTGATAAA+GGG - Chr7:88886936-88886955 None:intergenic 20.0%
!! AAGATAATGTAGTGAATATT+TGG - Chr7:88886294-88886313 None:intergenic 20.0%
!! AGATAATGTAGTGAATATTT+GGG - Chr7:88886293-88886312 None:intergenic 20.0%
!! ATAAATAAAGTGATGTTGAT+TGG - Chr7:88887324-88887343 None:intergenic 20.0%
!! TAAAGAAGGATAATTGATAA+AGG - Chr7:88886937-88886956 None:intergenic 20.0%
!! TAATGACAGACTTTAATTAA+TGG - Chr7:88887252-88887271 None:intergenic 20.0%
!! TCAAAAACTAAATTATCTAG+TGG - Chr7:88885991-88886010 None:intergenic 20.0%
!!! ATGTATTGGTATTTTTTAAG+AGG + Chr7:88887384-88887403 MsG0780041259.01.T01:intron 20.0%
!!! CATCACTTTATTTATCATAT+TGG + Chr7:88887329-88887348 MsG0780041259.01.T01:intron 20.0%
!!! GATGAATTACTTTTTTTGAA+AGG - Chr7:88886667-88886686 None:intergenic 20.0%
!!! TTTCTTAATTAGTGCTATTA+GGG - Chr7:88886076-88886095 None:intergenic 20.0%
!!! TTTTCTTAATTAGTGCTATT+AGG - Chr7:88886077-88886096 None:intergenic 20.0%
! AAATGAAAGGAAAATGCTAA+AGG - Chr7:88886152-88886171 None:intergenic 25.0%
! AATGAAAGGAAAATGCTAAA+GGG - Chr7:88886151-88886170 None:intergenic 25.0%
! ACTAAAATAGACATAGCTAA+AGG - Chr7:88886968-88886987 None:intergenic 25.0%
! ATATCTCTACAATTTCACAA+AGG + Chr7:88886687-88886706 MsG0780041259.01.T01:intron 25.0%
! TAAAGGAGAATTGATAAAGA+AGG - Chr7:88886951-88886970 None:intergenic 25.0%
! TAATTGATAAAGGGCAATAA+AGG - Chr7:88886927-88886946 None:intergenic 25.0%
! TATCTCTACAATTTCACAAA+GGG + Chr7:88886688-88886707 MsG0780041259.01.T01:intron 25.0%
!! CGGTATATTTTTCATTCAAA+AGG + Chr7:88886479-88886498 MsG0780041259.01.T01:intron 25.0%
!! TGAGAGAATTGATTTATGTT+TGG - Chr7:88887071-88887090 None:intergenic 25.0%
!! TGTTTTGTATTTTCCTTCAT+AGG + Chr7:88886035-88886054 MsG0780041259.01.T01:intron 25.0%
!! TTGATTGGTTAAGCTTTAAT+GGG - Chr7:88887309-88887328 None:intergenic 25.0%
!!! TTTTATAAAGTGTCGAATAC+AGG - Chr7:88887438-88887457 None:intergenic 25.0%
!!! TTTTTAAGAGGTGTGATATT+TGG + Chr7:88887396-88887415 MsG0780041259.01.T01:intron 25.0%
AATGCTAGACCTAGTTATAA+TGG + Chr7:88887733-88887752 MsG0780041259.01.T01:CDS 30.0%
AGAGTAATGCCATTATAACT+AGG - Chr7:88887745-88887764 None:intergenic 30.0%
CATTTATATTCCAAATAGGG+AGG - Chr7:88887367-88887386 None:intergenic 30.0%
GGGCAATAAAGGAAAATAAT+AGG - Chr7:88886916-88886935 None:intergenic 30.0%
TTCATATGCTGCAATTTCTA+TGG + Chr7:88887655-88887674 MsG0780041259.01.T01:CDS 30.0%
! ATTCTCTCTTAGGTTTTTCT+TGG + Chr7:88887613-88887632 MsG0780041259.01.T01:intron 30.0%
! GTTGATTGGTTAAGCTTTAA+TGG - Chr7:88887310-88887329 None:intergenic 30.0%
! TATTTGGGATAAAGAGAAGA+TGG - Chr7:88886278-88886297 None:intergenic 30.0%
!! CAGTCACTTTGAAATTACTT+AGG - Chr7:88887588-88887607 None:intergenic 30.0%
!! CTTCAAGGATAATTGCTTTT+GGG - Chr7:88886823-88886842 None:intergenic 30.0%
!! TGTTTATGATGTGTTGCTTA+GGG + Chr7:88885802-88885821 MsG0780041259.01.T01:CDS 30.0%
!! TTCAAGGATAATTGCTTTTG+GGG - Chr7:88886822-88886841 None:intergenic 30.0%
!!! ATAGTGTTGATGTCAGATTT+TGG + Chr7:88887532-88887551 MsG0780041259.01.T01:intron 30.0%
!!! TAGTGTTGATGTCAGATTTT+GGG + Chr7:88887533-88887552 MsG0780041259.01.T01:intron 30.0%
!!! TTTTTGGCTAGAATTGCTTT+TGG - Chr7:88886761-88886780 None:intergenic 30.0%
AAACACACACAAAGATGCAT+TGG + Chr7:88887162-88887181 MsG0780041259.01.T01:intron 35.0%
AAGGTGGTAGTACAAATGAA+AGG - Chr7:88886165-88886184 None:intergenic 35.0%
AATGCATCTTTGTGTGTGTT+TGG - Chr7:88887163-88887182 None:intergenic 35.0%
AATTCAAAGCTACTGCTAAG+TGG + Chr7:88885909-88885928 MsG0780041259.01.T01:CDS 35.0%
ATGTACTTAAGCTTGTGAGT+AGG + Chr7:88886567-88886586 MsG0780041259.01.T01:intron 35.0%
ATTCAAAGCTACTGCTAAGT+GGG + Chr7:88885910-88885929 MsG0780041259.01.T01:CDS 35.0%
TATATTCCAAATAGGGAGGA+AGG - Chr7:88887363-88887382 None:intergenic 35.0%
TGTACTTAAGCTTGTGAGTA+GGG + Chr7:88886568-88886587 MsG0780041259.01.T01:intron 35.0%
! CTTGTTAACTTGACCTATGA+AGG - Chr7:88886051-88886070 None:intergenic 35.0%
! GACTGACTTTATTCTCTCTT+AGG + Chr7:88887603-88887622 MsG0780041259.01.T01:intron 35.0%
! GCTTCAAGGATAATTGCTTT+TGG - Chr7:88886824-88886843 None:intergenic 35.0%
! TTCTAGAAAAGCTAGAAGCT+AGG - Chr7:88886854-88886873 None:intergenic 35.0%
! TTCTTGGTGACAAATGCTAT+AGG + Chr7:88887629-88887648 MsG0780041259.01.T01:CDS 35.0%
!! CACTGTAGAGAATTGCTTTT+GGG - Chr7:88886738-88886757 None:intergenic 35.0%
!! GCTTTGGTTCTATCTATCAT+TGG + Chr7:88887881-88887900 MsG0780041259.01.T01:CDS 35.0%
!! GTGTTTATGATGTGTTGCTT+AGG + Chr7:88885801-88885820 MsG0780041259.01.T01:CDS 35.0%
!! TCAAGGATAATTGCTTTTGG+GGG - Chr7:88886821-88886840 None:intergenic 35.0%
!! TGAACCAAACACGTTGTTTT+TGG - Chr7:88886777-88886796 None:intergenic 35.0%
!!! TAGTGCTGTTTTATTTCCAC+TGG - Chr7:88887702-88887721 None:intergenic 35.0%
AAACCAGTCAAAGACAAGTG+TGG + Chr7:88885730-88885749 MsG0780041259.01.T01:CDS 40.0%
AACAGTCATGTGACGACAAT+AGG - Chr7:88887861-88887880 None:intergenic 40.0%
AATTTCACAAAGGGCCTGTT+TGG + Chr7:88886697-88886716 MsG0780041259.01.T01:intron 40.0%
AGCTAGGATTTGTAGCTTCA+AGG - Chr7:88886838-88886857 None:intergenic 40.0%
CACAAGTAAAAGGAAGACAG+TGG - Chr7:88887909-88887928 None:intergenic 40.0%
CTAGCCAAAAACAACGTGTT+TGG + Chr7:88886770-88886789 MsG0780041259.01.T01:intron 40.0%
CTCACTGCTACTATCATTGT+GGG + Chr7:88885845-88885864 MsG0780041259.01.T01:CDS 40.0%
GGTAATAAGCTGAACCAAAC+AGG - Chr7:88886714-88886733 None:intergenic 40.0%
TCAACAATTGCAAGAACCAG+TGG + Chr7:88887683-88887702 MsG0780041259.01.T01:CDS 40.0%
TCTCACTGCTACTATCATTG+TGG + Chr7:88885844-88885863 MsG0780041259.01.T01:CDS 40.0%
TGTGAGTAGGGTTTAGTAGT+TGG + Chr7:88886580-88886599 MsG0780041259.01.T01:intron 40.0%
! CACTGTCTTCCTTTTACTTG+TGG + Chr7:88887907-88887926 MsG0780041259.01.T01:CDS 40.0%
! GCACTGTAGAGAATTGCTTT+TGG - Chr7:88886739-88886758 None:intergenic 40.0%
! GCTACTATCATTGTGGGTTT+TGG + Chr7:88885851-88885870 MsG0780041259.01.T01:CDS 40.0%
!! TATCATTGTGGGTTTTGGCA+AGG + Chr7:88885856-88885875 MsG0780041259.01.T01:CDS 40.0%
!! TGTAGAGAATTGCTTTTGGG+TGG - Chr7:88886735-88886754 None:intergenic 40.0%
CAGTCAAAGACAAGTGTGGA+TGG + Chr7:88885734-88885753 MsG0780041259.01.T01:CDS 45.0%
CATCCACACTTGTCTTTGAC+TGG - Chr7:88885736-88885755 None:intergenic 45.0%
CTAGTGGTAGCATAGTAGAG+AGG - Chr7:88885975-88885994 None:intergenic 45.0%
GAGAGTGAGCCACAAGTAAA+AGG - Chr7:88887919-88887938 None:intergenic 45.0%
GCATTACTCTTCTCAGCCAA+TGG + Chr7:88887755-88887774 MsG0780041259.01.T01:CDS 45.0%
TCACAGCGATGTGAAGGTTA+TGG + Chr7:88885760-88885779 MsG0780041259.01.T01:CDS 45.0%
! TCGTCACATGACTGTTGCTT+TGG + Chr7:88887865-88887884 MsG0780041259.01.T01:CDS 45.0%
!! GATGTGTTGCTTAGGGTTCT+TGG + Chr7:88885809-88885828 MsG0780041259.01.T01:CDS 45.0%
AAGGAAACTCGCACGTGCAA+TGG + Chr7:88887810-88887829 MsG0780041259.01.T01:CDS 50.0%
GTAGCTCCTTCCTCCCTATT+TGG + Chr7:88887354-88887373 MsG0780041259.01.T01:intron 50.0%
TGGAGTTCACAGCGATGTGA+AGG + Chr7:88885754-88885773 MsG0780041259.01.T01:CDS 50.0%
!! GGGTTTTGGCAAGGAGACTA+AGG + Chr7:88885865-88885884 MsG0780041259.01.T01:CDS 50.0%
!! GTGGGAGTGTTAGGACAGAA+AGG + Chr7:88887791-88887810 MsG0780041259.01.T01:CDS 50.0%
AATGGAGCTGCTGGTGCAGT+GGG + Chr7:88887773-88887792 MsG0780041259.01.T01:CDS 55.0%
TTCTCAGCCAATGGAGCTGC+TGG + Chr7:88887764-88887783 MsG0780041259.01.T01:CDS 55.0%
CAATGGAGCTGCTGGTGCAG+TGG + Chr7:88887772-88887791 MsG0780041259.01.T01:CDS 60.0%
CACTGCACCAGCAGCTCCAT+TGG - Chr7:88887774-88887793 None:intergenic 60.0%
!! GCTGGTGCAGTGGGAGTGTT+AGG + Chr7:88887782-88887801 MsG0780041259.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 88885725 88887970 88885725 ID=MsG0780041259.01;Name=MsG0780041259.01
Chr7 mRNA 88885725 88887970 88885725 ID=MsG0780041259.01.T01;Parent=MsG0780041259.01;Name=MsG0780041259.01.T01;_AED=0.40;_eAED=0.41;_QI=0|0|0|1|1|1|2|0|190
Chr7 exon 88885725 88885951 88885725 ID=MsG0780041259.01.T01:exon:3404;Parent=MsG0780041259.01.T01
Chr7 exon 88887625 88887970 88887625 ID=MsG0780041259.01.T01:exon:3405;Parent=MsG0780041259.01.T01
Chr7 CDS 88885725 88885951 88885725 ID=MsG0780041259.01.T01:cds;Parent=MsG0780041259.01.T01
Chr7 CDS 88887625 88887970 88887625 ID=MsG0780041259.01.T01:cds;Parent=MsG0780041259.01.T01
Gene Sequence

>MsG0780041259.01.T01

ATGGGAAACCAGTCAAAGACAAGTGTGGATGGAGTTCACAGCGATGTGAAGGTTATGGAGATGAGAACTAGAAGTAGTGTTTATGATGTGTTGCTTAGGGTTCTTGGTTTCTTGCTTACTCTCACTGCTACTATCATTGTGGGTTTTGGCAAGGAGACTAAGGTCATTTCATATGCTTCATTGCAATTCAAAGCTACTGCTAAGTGGGAATATATGTCTGCTATTGTGTTTTTCTTGGTGACAAATGCTATAGGATGTTCATATGCTGCAATTTCTATGGTGATTTCAACAATTGCAAGAACCAGTGGAAATAAAACAGCACTATTGATGATAACAATGCTAGACCTAGTTATAATGGCATTACTCTTCTCAGCCAATGGAGCTGCTGGTGCAGTGGGAGTGTTAGGACAGAAAGGAAACTCGCACGTGCAATGGATGAAAGTGTGCAATGTATTTGATGCCTATTGTCGTCACATGACTGTTGCTTTGGTTCTATCTATCATTGGTTCCACTGTCTTCCTTTTACTTGTGGCTCACTCTCTTTTCAAACTCCATTATTATAGATCAAACTAG

Protein sequence

>MsG0780041259.01.T01

MGNQSKTSVDGVHSDVKVMEMRTRSSVYDVLLRVLGFLLTLTATIIVGFGKETKVISYASLQFKATAKWEYMSAIVFFLVTNAIGCSYAAISMVISTIARTSGNKTALLMITMLDLVIMALLFSANGAAGAVGVLGQKGNSHVQWMKVCNVFDAYCRHMTVALVLSIIGSTVFLLLVAHSLFKLHYYRSN*