AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048362.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048362.01.T01 MTR_3g047390 90.305 361 21 2 1 347 1 361 0.0 680
MsG0080048362.01.T01 MTR_2g061380 42.135 356 169 7 15 348 9 349 9.48e-81 268
MsG0080048362.01.T01 MTR_7g005950 41.935 372 171 11 3 348 2 354 1.56e-79 265
MsG0080048362.01.T01 MTR_7g005950 41.935 372 171 11 3 348 2 354 2.07e-79 265
MsG0080048362.01.T01 MTR_1g116370 41.734 369 164 11 7 348 13 357 1.10e-74 251
MsG0080048362.01.T01 MTR_3g096200 41.530 366 171 11 9 348 7 355 1.78e-74 251
MsG0080048362.01.T01 MTR_8g093630 42.577 357 169 10 18 348 13 359 1.83e-71 242
MsG0080048362.01.T01 MTR_8g093630 42.577 357 169 10 18 348 13 359 1.99e-71 242
MsG0080048362.01.T01 MTR_1g101710 52.489 221 31 5 101 306 15 176 2.55e-63 199
MsG0080048362.01.T01 MTR_2g072160 39.481 347 157 11 33 348 54 378 8.66e-62 214
MsG0080048362.01.T01 MTR_1g101690 74.194 93 23 1 13 104 2 94 8.90e-41 139
MsG0080048362.01.T01 MTR_1g116470 31.214 346 186 11 43 348 37 370 3.19e-37 142
MsG0080048362.01.T01 MTR_1g116470 31.214 346 186 11 43 348 37 370 3.58e-37 142
MsG0080048362.01.T01 MTR_3g075180 29.972 357 185 11 43 348 41 383 7.18e-36 139
MsG0080048362.01.T01 MTR_3g075180 29.972 357 185 11 43 348 41 383 8.88e-36 138
MsG0080048362.01.T01 MTR_7g027890 48.507 134 65 2 36 165 31 164 9.35e-33 120
MsG0080048362.01.T01 MTR_4g078220 38.251 183 79 5 187 348 17 186 1.65e-27 114
MsG0080048362.01.T01 MTR_2g090375 35.359 181 81 6 189 348 14 179 7.65e-26 109
MsG0080048362.01.T01 MTR_7g006330 41.958 143 75 4 35 169 9 151 3.47e-25 100
MsG0080048362.01.T01 MTR_2g013580 35.000 180 82 6 189 348 29 193 1.16e-23 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048362.01.T01 AT3G59100 56.438 365 136 7 2 348 3 362 3.80e-125 395
MsG0080048362.01.T01 AT1G06490 56.836 373 134 6 1 348 1 371 2.02e-120 382
MsG0080048362.01.T01 AT1G06490 56.836 373 134 6 1 348 1 371 2.43e-120 382
MsG0080048362.01.T01 AT5G13000 42.473 372 172 9 1 348 1 354 1.32e-80 268
MsG0080048362.01.T01 AT5G13000 42.473 372 172 9 1 348 1 354 1.33e-80 268
MsG0080048362.01.T01 AT1G05570 43.662 355 161 9 17 348 12 350 4.07e-80 267
MsG0080048362.01.T01 AT1G05570 43.662 355 161 9 17 348 12 350 5.40e-80 266
MsG0080048362.01.T01 AT1G05570 43.662 355 161 9 17 348 12 350 5.40e-80 266
MsG0080048362.01.T01 AT1G05570 43.662 355 161 9 17 348 12 350 5.40e-80 266
MsG0080048362.01.T01 AT2G31960 41.690 355 168 7 17 348 12 350 2.05e-79 265
MsG0080048362.01.T01 AT2G13680 40.323 372 179 10 2 348 4 357 2.61e-79 265
MsG0080048362.01.T01 AT2G31960 41.690 355 168 7 17 348 12 350 2.67e-79 265
MsG0080048362.01.T01 AT2G31960 41.690 355 168 7 17 348 12 350 2.67e-79 265
MsG0080048362.01.T01 AT2G31960 41.690 355 168 7 17 348 12 350 2.67e-79 265
MsG0080048362.01.T01 AT2G31960 41.690 355 168 7 17 348 12 350 2.67e-79 265
MsG0080048362.01.T01 AT3G14570 40.811 370 167 11 9 348 24 371 2.25e-66 227
MsG0080048362.01.T01 AT3G14570 40.811 370 167 11 9 348 24 371 2.78e-66 227
MsG0080048362.01.T01 AT3G14570 40.811 370 167 11 9 348 24 371 3.25e-66 227
MsG0080048362.01.T01 AT5G36870 37.637 364 174 11 25 348 3 353 4.69e-61 212
MsG0080048362.01.T01 AT5G36870 37.637 364 174 11 25 348 3 353 4.69e-61 212
MsG0080048362.01.T01 AT5G36870 37.637 364 174 11 25 348 3 353 5.05e-61 212
MsG0080048362.01.T01 AT5G36870 36.467 351 169 10 39 348 24 361 2.35e-54 192
MsG0080048362.01.T01 AT5G36870 36.467 351 169 10 39 348 24 361 2.58e-54 192
MsG0080048362.01.T01 AT3G07160 33.604 369 180 15 23 347 18 365 1.12e-37 144
MsG0080048362.01.T01 AT3G07160 33.604 369 180 15 23 347 18 365 1.12e-37 144
MsG0080048362.01.T01 AT3G07160 33.604 369 180 15 23 347 18 365 1.15e-37 144
MsG0080048362.01.T01 AT2G36850 27.865 384 207 12 20 348 10 378 4.39e-33 130
MsG0080048362.01.T01 AT4G03550 36.667 180 84 4 187 349 26 192 1.67e-27 114
MsG0080048362.01.T01 AT4G04970 33.155 187 86 5 187 348 18 190 9.21e-25 106
MsG0080048362.01.T01 AT3G14780 42.361 144 70 4 39 169 48 191 1.50e-24 102
MsG0080048362.01.T01 AT3G59100 64.615 65 23 0 284 348 1 65 2.16e-24 105

Find 66 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGCACTTTATCTTCTAATA+TGG 0.131084 contig283end:-5209 MsG0080048362.01.T01:CDS
TTCTCTTCTCGTAGAAATAT+TGG 0.303247 contig283end:+6664 None:intergenic
CGCACTTTATCTTCTAATAT+GGG 0.310836 contig283end:-5208 MsG0080048362.01.T01:CDS
GCCAAATGAAGAAAATATTA+TGG 0.322968 contig283end:-8222 MsG0080048362.01.T01:CDS
AAAATATAGCAAAGACATTC+AGG 0.332666 contig283end:+5159 None:intergenic
TCAATAGCTGCTCTAAGAAT+TGG 0.374964 contig283end:+8161 None:intergenic
GCCCTTATCATCCTCTTTGA+AGG 0.381578 contig283end:+8266 None:intergenic
TGCGTTGTAAATCAAATATT+AGG 0.409028 contig283end:-5368 MsG0080048362.01.T01:intron
TTACTTCTGTAGGGAGGAAA+TGG 0.410527 contig283end:-6531 MsG0080048362.01.T01:intron
ACCTTCTCCTCAATAGTTTG+TGG 0.420420 contig283end:+6452 None:intergenic
TTAACACCGACAGCATATAA+TGG 0.426554 contig283end:+6116 None:intergenic
TCAGGTCTTGAATGAATGAT+AGG 0.427078 contig283end:+5912 None:intergenic
AACCTTCAAAGAGGATGATA+AGG 0.427163 contig283end:-8268 MsG0080048362.01.T01:CDS
CTGCAGATGTTGAGAATAAG+AGG 0.433173 contig283end:-6166 MsG0080048362.01.T01:CDS
ATTGTAGAATCATCATTATC+AGG 0.437197 contig283end:+5894 None:intergenic
AGAGCAGCTATTGAGATTGA+AGG 0.440766 contig283end:-8152 MsG0080048362.01.T01:CDS
ATGTGAATGATATACTTGAT+TGG 0.444779 contig283end:-5851 MsG0080048362.01.T01:CDS
ATTTCTACGAGAAGAGAATC+CGG 0.446906 contig283end:-6659 MsG0080048362.01.T01:CDS
AAAAGGACCCTTTGTCCCAC+TGG 0.449533 contig283end:+8304 None:intergenic
GGCAACTCAACAGTCCTTGT+AGG 0.450187 contig283end:+8242 None:intergenic
CCTGTTGCACAAACTCGAAA+AGG 0.465245 contig283end:-7196 MsG0080048362.01.T01:intron
CTTGAATGAATGATAGGCAT+TGG 0.478017 contig283end:+5918 None:intergenic
CGGTGTTAAACCAGTGATCA+TGG 0.485594 contig283end:-6102 MsG0080048362.01.T01:CDS
TGATGATTCTACAATGCCTA+TGG 0.488335 contig283end:-5886 MsG0080048362.01.T01:CDS
GAGCAGCTATTGAGATTGAA+GGG 0.498050 contig283end:-8151 MsG0080048362.01.T01:CDS
ACAAGAACAGCAAGAGAAGA+AGG 0.498507 contig283end:+8185 None:intergenic
ACTTGCTGCTTTAAGTAAAG+TGG 0.500372 contig283end:-5949 MsG0080048362.01.T01:CDS
AGATACTATAGAGAAGTTGA+TGG 0.509694 contig283end:-5430 MsG0080048362.01.T01:CDS
GGTTGTCTAGAGATTTCAAA+AGG 0.510254 contig283end:+8287 None:intergenic
GCTAACTCCACAAACTATTG+AGG 0.510656 contig283end:-6459 MsG0080048362.01.T01:CDS
TTGAATGAATGATAGGCATT+GGG 0.514101 contig283end:+5919 None:intergenic
ACTTCTCTATAGTATCTTCA+CGG 0.515373 contig283end:+5436 None:intergenic
AGAAACATGGCTAGCACCAG+TGG 0.521758 contig283end:-8320 None:intergenic
AGATTGAAGGGGAGAATCCA+AGG 0.525316 contig283end:-8139 MsG0080048362.01.T01:CDS
GGCTAGCACCAGTGGGACAA+AGG 0.530271 contig283end:-8312 MsG0080048362.01.T01:CDS
TCGGGAAGTTCCATGATCAC+TGG 0.532270 contig283end:+6092 None:intergenic
TCTGTAGGGAGGAAATGGTT+AGG 0.535955 contig283end:-6526 MsG0080048362.01.T01:intron
ATTTGAGAAGGCTCATACAA+TGG 0.539983 contig283end:-7262 MsG0080048362.01.T01:CDS
TCACAAAGATAAGCAACCCT+TGG 0.542873 contig283end:+8122 None:intergenic
GCTAGCACCAGTGGGACAAA+GGG 0.550353 contig283end:-8311 MsG0080048362.01.T01:CDS
ACACCACGCCCACTTGATGT+TGG 0.553931 contig283end:+7237 None:intergenic
TCCACAAACTATTGAGGAGA+AGG 0.555031 contig283end:-6453 MsG0080048362.01.T01:intron
TCGCTTTCATGCATTTGAGA+AGG 0.555337 contig283end:-7274 MsG0080048362.01.T01:CDS
TTTCTACGAGAAGAGAATCC+GGG 0.557673 contig283end:-6658 MsG0080048362.01.T01:CDS
GGATGATAAGGGCACCTACA+AGG 0.560664 contig283end:-8256 MsG0080048362.01.T01:CDS
TTTAACAATAGGTATTCAGA+TGG 0.565269 contig283end:-5264 MsG0080048362.01.T01:intron
GTTACGGACAACTTACATGA+TGG 0.568511 contig283end:+5136 None:intergenic
ACCTTCAAAGAGGATGATAA+GGG 0.578884 contig283end:-8267 MsG0080048362.01.T01:CDS
GCACTTTATCTTCTAATATG+GGG 0.581111 contig283end:-5207 MsG0080048362.01.T01:CDS
ATTCTTCCATTATATGCTGT+CGG 0.581398 contig283end:-6122 MsG0080048362.01.T01:CDS
TTCTACAATGCCTATGGAGA+GGG 0.582192 contig283end:-5880 MsG0080048362.01.T01:CDS
TACTAAAAGAAGTGATGCGA+GGG 0.584970 contig283end:-6703 MsG0080048362.01.T01:CDS
GATCCAACATCAAGTGGGCG+TGG 0.587360 contig283end:-7240 MsG0080048362.01.T01:CDS
TCTCTAGACAACCTTCAAAG+AGG 0.601199 contig283end:-8277 MsG0080048362.01.T01:CDS
ACAATGGATCCAACATCAAG+TGG 0.601462 contig283end:-7246 MsG0080048362.01.T01:CDS
AGCAGCTATTGAGATTGAAG+GGG 0.604758 contig283end:-8150 MsG0080048362.01.T01:CDS
AGTGATCATGGAACTTCCCG+AGG 0.620491 contig283end:-6090 MsG0080048362.01.T01:intron
TGCAGATGTTGAGAATAAGA+GGG 0.634983 contig283end:-6165 MsG0080048362.01.T01:CDS
ATTCTACAATGCCTATGGAG+AGG 0.635776 contig283end:-5881 MsG0080048362.01.T01:CDS
ATACTAAAAGAAGTGATGCG+AGG 0.659853 contig283end:-6704 MsG0080048362.01.T01:CDS
TACGAGAAGAGAATCCGGGA+TGG 0.662323 contig283end:-6654 MsG0080048362.01.T01:CDS
GATTGAAGGGGAGAATCCAA+GGG 0.663786 contig283end:-8138 MsG0080048362.01.T01:CDS
CAATGGATCCAACATCAAGT+GGG 0.671337 contig283end:-7245 MsG0080048362.01.T01:CDS
GAAACATGGCTAGCACCAGT+GGG 0.684163 contig283end:-8319 None:intergenic
GCAGATGTTGAGAATAAGAG+GGG 0.696593 contig283end:-6164 MsG0080048362.01.T01:CDS
CTGTGTTGTATGAAGTACTG+AGG 0.755476 contig283end:-6487 MsG0080048362.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAAAAAAGAC+GGG + contig283end:7200-7219 None:intergenic 10.0%
!! ATAAATTAGAAAAACAAAAA+TGG - contig283end:6055-6074 MsG0080048362.01.T01:intron 10.0%
!! ATAATATAATTAAGCAAAAA+TGG + contig283end:5377-5396 None:intergenic 10.0%
!! ATTAAATTTAATTGTAGTTA+GGG - contig283end:5654-5673 MsG0080048362.01.T01:intron 10.0%
!! TTATTCAAAAAACAAAAAAT+GGG + contig283end:5907-5926 None:intergenic 10.0%
!! TTTATTCAAAAAACAAAAAA+TGG + contig283end:5908-5927 None:intergenic 10.0%
!!! TTTATTTTCTTTGTTTAATT+AGG - contig283end:6154-6173 MsG0080048362.01.T01:CDS 10.0%
!! AATACACAAAAACATTAAAT+TGG + contig283end:7745-7764 None:intergenic 15.0%
!! ACTCATAAAAAACAAAAAAT+GGG + contig283end:5422-5441 None:intergenic 15.0%
!! GATTAAATTTAATTGTAGTT+AGG - contig283end:5653-5672 MsG0080048362.01.T01:intron 15.0%
!!! TGCATGATTATAATTTTTTA+GGG - contig283end:5776-5795 MsG0080048362.01.T01:intron 15.0%
!!! TTTAATGTTTTTGTGTATTT+TGG - contig283end:7746-7765 MsG0080048362.01.T01:intron 15.0%
!!! TTTTTTATAATAAGAATAGC+AGG - contig283end:7217-7236 MsG0080048362.01.T01:CDS 15.0%
!! AAAAGAGATATTCAAATTGA+AGG + contig283end:7683-7702 None:intergenic 20.0%
!! AAAGAGATATTCAAATTGAA+GGG + contig283end:7682-7701 None:intergenic 20.0%
!! AAATACACAAGCAAATTATT+AGG + contig283end:7442-7461 None:intergenic 20.0%
!! ACTCATTCTCTTTATATATA+AGG - contig283end:6508-6527 MsG0080048362.01.T01:CDS 20.0%
!! CACTCATAAAAAACAAAAAA+TGG + contig283end:5423-5442 None:intergenic 20.0%
!! TATAATTTCATAGAAAAGAG+AGG + contig283end:7470-7489 None:intergenic 20.0%
!! TGGAATAATCTAAATATAGA+GGG - contig283end:7060-7079 MsG0080048362.01.T01:intron 20.0%
!! TTAACATTTGACAGAAATTA+TGG - contig283end:6585-6604 MsG0080048362.01.T01:intron 20.0%
!!! AAAGAAGAAAACTTTTATGA+GGG - contig283end:5808-5827 MsG0080048362.01.T01:CDS 20.0%
!!! CATGATTATAATTTTTTAGG+GGG - contig283end:5778-5797 MsG0080048362.01.T01:intron 20.0%
!!! CTGCATGATTATAATTTTTT+AGG - contig283end:5775-5794 MsG0080048362.01.T01:intron 20.0%
!!! GAAATTTTCATTAACTAACA+TGG + contig283end:7171-7190 None:intergenic 20.0%
!!! GCATGATTATAATTTTTTAG+GGG - contig283end:5777-5796 MsG0080048362.01.T01:intron 20.0%
!!! GTATTTTGGAATAAAAAGAA+AGG - contig283end:7760-7779 MsG0080048362.01.T01:intron 20.0%
!!! TAAAGAAGAAAACTTTTATG+AGG - contig283end:5807-5826 MsG0080048362.01.T01:CDS 20.0%
!!! TATGCAATTTGTTTAACAAT+AGG - contig283end:8176-8195 MsG0080048362.01.T01:CDS 20.0%
!!! TCCATAATATTTTCTTCATT+TGG + contig283end:5233-5252 None:intergenic 20.0%
! AAAAAAAAAAAAAGACGGGA+AGG + contig283end:7196-7215 None:intergenic 25.0%
! AAAAAAAAAAAAGACGGGAA+GGG + contig283end:7195-7214 None:intergenic 25.0%
! AAAATATAGCAAAGACATTC+AGG + contig283end:8295-8314 None:intergenic 25.0%
! AACTATTTCAAAACATTTGC+TGG - contig283end:6696-6715 MsG0080048362.01.T01:CDS 25.0%
! AATTACACACAAAATTTCCA+AGG + contig283end:5469-5488 None:intergenic 25.0%
! AATTTAATTGTAAACCCACT+TGG - contig283end:5512-5531 MsG0080048362.01.T01:intron 25.0%
! AGTGTTAAAGTTATACACTA+TGG - contig283end:5439-5458 MsG0080048362.01.T01:CDS 25.0%
! ATGTGAATGATATACTTGAT+TGG - contig283end:7600-7619 MsG0080048362.01.T01:intron 25.0%
! ATTGTAGAATCATCATTATC+AGG + contig283end:7560-7579 None:intergenic 25.0%
! ATTTAATTGTAAACCCACTT+GGG - contig283end:5513-5532 MsG0080048362.01.T01:intron 25.0%
! CTTTATTGATCACAAAAGAA+TGG - contig283end:6407-6426 MsG0080048362.01.T01:intron 25.0%
! GAATAAAAAGAAAGGAAATG+TGG - contig283end:7768-7787 MsG0080048362.01.T01:intron 25.0%
! GAATATACTAAAAAACCGTA+AGG - contig283end:6821-6840 MsG0080048362.01.T01:intron 25.0%
! GATGTATTAAGTAACTAGTT+AGG + contig283end:6084-6103 None:intergenic 25.0%
! GCCAAATGAAGAAAATATTA+TGG - contig283end:5229-5248 MsG0080048362.01.T01:CDS 25.0%
! GTGGAATAATCTAAATATAG+AGG - contig283end:7059-7078 MsG0080048362.01.T01:intron 25.0%
! TATTCCACAAATTTAAGTGA+TGG + contig283end:7047-7066 None:intergenic 25.0%
! TGCGTTGTAAATCAAATATT+AGG - contig283end:8083-8102 MsG0080048362.01.T01:intron 25.0%
! TTCATTCTGTTAAGTTTCTA+AGG - contig283end:6344-6363 MsG0080048362.01.T01:intron 25.0%
! TTTAACAATAGGTATTCAGA+TGG - contig283end:8187-8206 MsG0080048362.01.T01:CDS 25.0%
!! AAGAAGAAAACTTTTATGAG+GGG - contig283end:5809-5828 MsG0080048362.01.T01:CDS 25.0%
!! GCTTTTTGAACGTAATATAT+TGG - contig283end:7904-7923 MsG0080048362.01.T01:intron 25.0%
!! TCTCATTTTCATATGAACAA+TGG + contig283end:5951-5970 None:intergenic 25.0%
!!! ATTATAATTTTTTAGGGGGT+AGG - contig283end:5782-5801 MsG0080048362.01.T01:intron 25.0%
!!! CTCATAATTTTTGAAAGTTG+TGG + contig283end:8047-8066 None:intergenic 25.0%
!!! TTTCTAAGGTAGATCAATTT+AGG - contig283end:6358-6377 MsG0080048362.01.T01:intron 25.0%
AAAAAAAAAAAGACGGGAAG+GGG + contig283end:7194-7213 None:intergenic 30.0%
AACAATTCAAAAACCAGAGT+TGG + contig283end:5977-5996 None:intergenic 30.0%
AATGAGTCAGAGTGAAATTT+AGG + contig283end:8150-8169 None:intergenic 30.0%
AATTTCACTCTGACTCATTT+CGG - contig283end:8151-8170 MsG0080048362.01.T01:CDS 30.0%
ACACAAGCAAATTATTAGGA+AGG + contig283end:7438-7457 None:intergenic 30.0%
ACTTCTCTATAGTATCTTCA+CGG + contig283end:8018-8037 None:intergenic 30.0%
AGATACTATAGAGAAGTTGA+TGG - contig283end:8021-8040 MsG0080048362.01.T01:intron 30.0%
AGGTAGATCAATTTAGGTAA+AGG - contig283end:6364-6383 MsG0080048362.01.T01:intron 30.0%
AGTTTGAACTAGAAGAAATC+CGG + contig283end:6458-6477 None:intergenic 30.0%
ATTCTTCCATTATATGCTGT+CGG - contig283end:7329-7348 MsG0080048362.01.T01:intron 30.0%
CATTACCTGAAATCCAAAAA+TGG + contig283end:7633-7652 None:intergenic 30.0%
CGCACTTTATCTTCTAATAT+GGG - contig283end:8243-8262 MsG0080048362.01.T01:CDS 30.0%
CTTCTGATATTACTTCTGTA+GGG - contig283end:6911-6930 MsG0080048362.01.T01:intron 30.0%
CTTTCAAAAATTATGAGTCG+TGG - contig283end:8050-8069 MsG0080048362.01.T01:intron 30.0%
GATTTCAAATCTTTACTGCT+TGG + contig283end:5734-5753 None:intergenic 30.0%
GCACTTTATCTTCTAATATG+GGG - contig283end:8244-8263 MsG0080048362.01.T01:CDS 30.0%
GGTAGATCAATTTAGGTAAA+GGG - contig283end:6365-6384 MsG0080048362.01.T01:intron 30.0%
GTTACCATCACTTAAATTTG+TGG - contig283end:7040-7059 MsG0080048362.01.T01:intron 30.0%
TCGCACTTTATCTTCTAATA+TGG - contig283end:8242-8261 MsG0080048362.01.T01:CDS 30.0%
TCTTCTGATATTACTTCTGT+AGG - contig283end:6910-6929 MsG0080048362.01.T01:intron 30.0%
TGTAGCAACTAAGAAATGAA+CGG + contig283end:6646-6665 None:intergenic 30.0%
TGTTGGCAAGCAATAATATA+AGG + contig283end:7810-7829 None:intergenic 30.0%
TTAGTTGCTACAAGAAAAAG+AGG - contig283end:6654-6673 MsG0080048362.01.T01:CDS 30.0%
TTATACACTATGGATTTCCT+TGG - contig283end:5449-5468 MsG0080048362.01.T01:CDS 30.0%
TTCTCTTCTCGTAGAAATAT+TGG + contig283end:6790-6809 None:intergenic 30.0%
TTGAATGAATGATAGGCATT+GGG + contig283end:7535-7554 None:intergenic 30.0%
! AGAAGAAAACTTTTATGAGG+GGG - contig283end:5810-5829 MsG0080048362.01.T01:CDS 30.0%
! ATTTTCTGTTTGTGTCAGTT+AGG + contig283end:6560-6579 None:intergenic 30.0%
! TTTTAGTATATTCACCATCC+CGG + contig283end:6814-6833 None:intergenic 30.0%
! TTTTTGAATAAAGATGCTGC+TGG - contig283end:5916-5935 MsG0080048362.01.T01:CDS 30.0%
AAAAAAAAAAGACGGGAAGG+GGG + contig283end:7193-7212 None:intergenic 35.0%
AAATTGACAATTGACACCAG+AGG + contig283end:5604-5623 None:intergenic 35.0%
AACCTTCAAAGAGGATGATA+AGG - contig283end:5183-5202 MsG0080048362.01.T01:CDS 35.0%
AAGATTGTAAAACGGCCATT+AGG - contig283end:7144-7163 MsG0080048362.01.T01:intron 35.0%
AAGGAAATGTGGCAAATCAA+AGG - contig283end:7779-7798 MsG0080048362.01.T01:intron 35.0%
AATTGACAATTGACACCAGA+GGG + contig283end:5603-5622 None:intergenic 35.0%
ACCTTCAAAGAGGATGATAA+GGG - contig283end:5184-5203 MsG0080048362.01.T01:CDS 35.0%
AGATTGTAAAACGGCCATTA+GGG - contig283end:7145-7164 MsG0080048362.01.T01:intron 35.0%
AGGAAATGTGGCAAATCAAA+GGG - contig283end:7780-7799 MsG0080048362.01.T01:intron 35.0%
ATACTAAAAGAAGTGATGCG+AGG - contig283end:6747-6766 MsG0080048362.01.T01:intron 35.0%
ATTAACTAACATGGCCCTAA+TGG + contig283end:7162-7181 None:intergenic 35.0%
ATTTAAATTTGGCACTACCC+TGG + contig283end:7934-7953 None:intergenic 35.0%
ATTTCTACGAGAAGAGAATC+CGG - contig283end:6792-6811 MsG0080048362.01.T01:intron 35.0%
ATTTGAGAAGGCTCATACAA+TGG - contig283end:6189-6208 MsG0080048362.01.T01:intron 35.0%
CCAACATAGACATAAGGAAT+AGG - contig283end:7824-7843 MsG0080048362.01.T01:intron 35.0%
CCTATTCCTTATGTCTATGT+TGG + contig283end:7827-7846 None:intergenic 35.0%
CTAAATATAGAGGGTCTATG+AGG - contig283end:7069-7088 MsG0080048362.01.T01:intron 35.0%
CTTGAATGAATGATAGGCAT+TGG + contig283end:7536-7555 None:intergenic 35.0%
CTTGCGAGAAGATTGTAAAA+CGG - contig283end:7136-7155 MsG0080048362.01.T01:intron 35.0%
GAAAATGAGAAAACCAACTC+TGG - contig283end:5961-5980 MsG0080048362.01.T01:CDS 35.0%
GGTTGTCTAGAGATTTCAAA+AGG + contig283end:5167-5186 None:intergenic 35.0%
GTAAGATCAATCAAGCCTTA+CGG + contig283end:6839-6858 None:intergenic 35.0%
TAATTTACCTCATGAGATGC+AGG + contig283end:7850-7869 None:intergenic 35.0%
TACCTGAAATCCAAAAATGG+AGG + contig283end:7630-7649 None:intergenic 35.0%
TACTAAAAGAAGTGATGCGA+GGG - contig283end:6748-6767 MsG0080048362.01.T01:intron 35.0%
TCAGGTCTTGAATGAATGAT+AGG + contig283end:7542-7561 None:intergenic 35.0%
TGATGATTCTACAATGCCTA+TGG - contig283end:7565-7584 MsG0080048362.01.T01:intron 35.0%
TGCAGATGTTGAGAATAAGA+GGG - contig283end:7286-7305 MsG0080048362.01.T01:intron 35.0%
TGTCAATTGTCAATTTGGGT+GGG - contig283end:5608-5627 MsG0080048362.01.T01:intron 35.0%
TGTGTCAGTTAGGAGTTAAT+TGG + contig283end:6550-6569 None:intergenic 35.0%
TTAACACCGACAGCATATAA+TGG + contig283end:7338-7357 None:intergenic 35.0%
TTGTGATCAATAAAGCTGCA+GGG + contig283end:6402-6421 None:intergenic 35.0%
TTTGTGATCAATAAAGCTGC+AGG + contig283end:6403-6422 None:intergenic 35.0%
! ACATTTTTAACCCTCTCCAT+AGG + contig283end:7584-7603 None:intergenic 35.0%
! CTGGTGTCAATTGTCAATTT+GGG - contig283end:5604-5623 MsG0080048362.01.T01:intron 35.0%
! GTTTTACAATCTTCTCGCAA+GGG + contig283end:7137-7156 None:intergenic 35.0%
! TCTGGTGTCAATTGTCAATT+TGG - contig283end:5603-5622 MsG0080048362.01.T01:intron 35.0%
! TTTTACAATCTTCTCGCAAG+GGG + contig283end:7136-7155 None:intergenic 35.0%
!! ACTTGCTGCTTTAAGTAAAG+TGG - contig283end:7502-7521 MsG0080048362.01.T01:intron 35.0%
!! TCAATAGCTGCTCTAAGAAT+TGG + contig283end:5293-5312 None:intergenic 35.0%
AAAGAGAATGAGTCAGCTTC+AGG + contig283end:6501-6520 None:intergenic 40.0%
AAGAGAATGAGTCAGCTTCA+GGG + contig283end:6500-6519 None:intergenic 40.0%
AATTGTAAACCCACTTGGGT+TGG - contig283end:5517-5536 MsG0080048362.01.T01:intron 40.0%
ACAAGAACAGCAAGAGAAGA+AGG + contig283end:5269-5288 None:intergenic 40.0%
ACAATGGATCCAACATCAAG+TGG - contig283end:6205-6224 MsG0080048362.01.T01:intron 40.0%
ACCTTCTCCTCAATAGTTTG+TGG + contig283end:7002-7021 None:intergenic 40.0%
ACGTAATATATTGGACAGCC+AGG - contig283end:7913-7932 MsG0080048362.01.T01:intron 40.0%
AGAGCAGCTATTGAGATTGA+AGG - contig283end:5299-5318 MsG0080048362.01.T01:intron 40.0%
AGCAGCTATTGAGATTGAAG+GGG - contig283end:5301-5320 MsG0080048362.01.T01:intron 40.0%
ATTCTACAATGCCTATGGAG+AGG - contig283end:7570-7589 MsG0080048362.01.T01:intron 40.0%
CAATGGATCCAACATCAAGT+GGG - contig283end:6206-6225 MsG0080048362.01.T01:intron 40.0%
CGAGATTCTAATCTTTCCAC+CGG - contig283end:6436-6455 MsG0080048362.01.T01:intron 40.0%
CGTAATATATTGGACAGCCA+GGG - contig283end:7914-7933 MsG0080048362.01.T01:intron 40.0%
CTGATATTACTTCTGTAGGG+AGG - contig283end:6914-6933 MsG0080048362.01.T01:intron 40.0%
CTGCAGATGTTGAGAATAAG+AGG - contig283end:7285-7304 MsG0080048362.01.T01:intron 40.0%
GAACAATGATGAGTATCTCG+TGG - contig283end:6320-6339 MsG0080048362.01.T01:intron 40.0%
GAATCGAACATAAGACCTTG+AGG + contig283end:5581-5600 None:intergenic 40.0%
GAGCAGCTATTGAGATTGAA+GGG - contig283end:5300-5319 MsG0080048362.01.T01:intron 40.0%
GCAGATGTTGAGAATAAGAG+GGG - contig283end:7287-7306 MsG0080048362.01.T01:intron 40.0%
GCTAACTCCACAAACTATTG+AGG - contig283end:6992-7011 MsG0080048362.01.T01:intron 40.0%
GTGTCAATTGTCAATTTGGG+TGG - contig283end:5607-5626 MsG0080048362.01.T01:intron 40.0%
TCACAAAGATAAGCAACCCT+TGG + contig283end:5332-5351 None:intergenic 40.0%
TCCACAAACTATTGAGGAGA+AGG - contig283end:6998-7017 MsG0080048362.01.T01:intron 40.0%
TCGCTTTCATGCATTTGAGA+AGG - contig283end:6177-6196 MsG0080048362.01.T01:CDS 40.0%
TCTCTAGACAACCTTCAAAG+AGG - contig283end:5174-5193 MsG0080048362.01.T01:CDS 40.0%
TGCTTGCCAACATAGACATA+AGG - contig283end:7818-7837 MsG0080048362.01.T01:intron 40.0%
TGTATGCGACATACAAACCT+CGG + contig283end:7381-7400 None:intergenic 40.0%
TTACTTCTGTAGGGAGGAAA+TGG - contig283end:6920-6939 MsG0080048362.01.T01:intron 40.0%
TTCTACAATGCCTATGGAGA+GGG - contig283end:7571-7590 MsG0080048362.01.T01:intron 40.0%
TTGAACTAGAAGAAATCCGG+TGG + contig283end:6455-6474 None:intergenic 40.0%
TTTCTACGAGAAGAGAATCC+GGG - contig283end:6793-6812 MsG0080048362.01.T01:intron 40.0%
! CAAGAAAAAGAGGCTCTAGT+TGG - contig283end:6664-6683 MsG0080048362.01.T01:CDS 40.0%
! CGTTTTACAATCTTCTCGCA+AGG + contig283end:7138-7157 None:intergenic 40.0%
! CTGCAGCATATCTTTTTGTC+TGG + contig283end:7271-7290 None:intergenic 40.0%
! GATTGGATTGCCTCCATTTT+TGG - contig283end:7617-7636 MsG0080048362.01.T01:intron 40.0%
! GCTTGGTCTATTTTACTGCT+TGG + contig283end:5717-5736 None:intergenic 40.0%
!! CTGTGTTGTATGAAGTACTG+AGG - contig283end:6964-6983 MsG0080048362.01.T01:intron 40.0%
!! TGCCTCCATTTTTGGATTTC+AGG - contig283end:7625-7644 MsG0080048362.01.T01:intron 40.0%
AGAGAATGAGTCAGCTTCAG+GGG + contig283end:6499-6518 None:intergenic 45.0%
AGATTGAAGGGGAGAATCCA+AGG - contig283end:5312-5331 MsG0080048362.01.T01:intron 45.0%
AGTCTCAAGCCAATACCACT+AGG + contig283end:5543-5562 None:intergenic 45.0%
CCTGTTGCACAAACTCGAAA+AGG - contig283end:6255-6274 MsG0080048362.01.T01:intron 45.0%
GATTGAAGGGGAGAATCCAA+GGG - contig283end:5313-5332 MsG0080048362.01.T01:intron 45.0%
GCCCTTATCATCCTCTTTGA+AGG + contig283end:5188-5207 None:intergenic 45.0%
GTATGCGACATACAAACCTC+GGG + contig283end:7380-7399 None:intergenic 45.0%
GTCTTATGTTCGATTCCCTC+TGG - contig283end:5585-5604 MsG0080048362.01.T01:intron 45.0%
TCGAACATAAGACCTTGAGG+AGG + contig283end:5578-5597 None:intergenic 45.0%
TCTATGAGGTTTCAGCTCAG+TGG - contig283end:7083-7102 MsG0080048362.01.T01:intron 45.0%
TCTGTAGGGAGGAAATGGTT+AGG - contig283end:6925-6944 MsG0080048362.01.T01:intron 45.0%
TGATCAATAAAGCTGCAGGG+AGG + contig283end:6399-6418 None:intergenic 45.0%
! CGGTGTTAAACCAGTGATCA+TGG - contig283end:7349-7368 MsG0080048362.01.T01:intron 45.0%
! TCAAAACATTTGCTGGCTGC+AGG - contig283end:6703-6722 MsG0080048362.01.T01:CDS 45.0%
!! AGTGGTATTGGCTTGAGACT+TGG - contig283end:5543-5562 MsG0080048362.01.T01:intron 45.0%
!! GTGGTATTGGCTTGAGACTT+GGG - contig283end:5544-5563 MsG0080048362.01.T01:intron 45.0%
!!! CCTTTTCGAGTTTGTGCAAC+AGG + contig283end:6258-6277 None:intergenic 45.0%
!! AAAAAAAAAAAAAAAAAAGA+CGG + contig283end:7201-7220 None:intergenic 5.0%
!! ATAATTAAAGAATTTAAATT+TGG + contig283end:7945-7964 None:intergenic 5.0%
AAAAGGACCCTTTGTCCCAC+TGG + contig283end:5150-5169 None:intergenic 50.0%
AGTGATCATGGAACTTCCCG+AGG - contig283end:7361-7380 MsG0080048362.01.T01:intron 50.0%
GAATAGGCCTGCATCTCATG+AGG - contig283end:7840-7859 MsG0080048362.01.T01:intron 50.0%
GGCAACTCAACAGTCCTTGT+AGG + contig283end:5212-5231 None:intergenic 50.0%
TACGAGAAGAGAATCCGGGA+TGG - contig283end:6797-6816 MsG0080048362.01.T01:intron 50.0%
TCGGGAAGTTCCATGATCAC+TGG + contig283end:7362-7381 None:intergenic 50.0%
TCTTCTCGCAAGGGGAGTTA+AGG + contig283end:7128-7147 None:intergenic 50.0%
! AACATTTGCTGGCTGCAGGA+AGG - contig283end:6707-6726 MsG0080048362.01.T01:CDS 50.0%
!! GGATGATAAGGGCACCTACA+AGG - contig283end:5195-5214 MsG0080048362.01.T01:CDS 50.0%
!! TTGGGTTGGCCTAGTGGTAT+TGG - contig283end:5531-5550 MsG0080048362.01.T01:intron 50.0%
ACACCACGCCCACTTGATGT+TGG + contig283end:6217-6236 None:intergenic 55.0%
ACCACTAGGCCAACCCAAGT+GGG + contig283end:5529-5548 None:intergenic 55.0%
GATCCAACATCAAGTGGGCG+TGG - contig283end:6211-6230 MsG0080048362.01.T01:intron 55.0%
GCTAGCACCAGTGGGACAAA+GGG - contig283end:5140-5159 MsG0080048362.01.T01:CDS 55.0%
TACCACTAGGCCAACCCAAG+TGG + contig283end:5530-5549 None:intergenic 55.0%
! TGGGAGTATGCTCCTCCTCA+AGG - contig283end:5563-5582 MsG0080048362.01.T01:intron 55.0%
GGCTAGCACCAGTGGGACAA+AGG - contig283end:5139-5158 MsG0080048362.01.T01:CDS 60.0%
!! ACCCACTTGGGTTGGCCTAG+TGG - contig283end:5525-5544 MsG0080048362.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
contig283end gene 5137 8336 5137 ID=MsG0080048362.01;Name=MsG0080048362.01
contig283end mRNA 5137 8336 5137 ID=MsG0080048362.01.T01;Parent=MsG0080048362.01;Name=MsG0080048362.01.T01;_AED=0.47;_eAED=0.48;_QI=0|0.85|0.5|1|0.85|0.75|8|0|350
contig283end exon 8126 8336 8126 ID=MsG0080048362.01.T01:exon:24812;Parent=MsG0080048362.01.T01
contig283end exon 7197 7297 7197 ID=MsG0080048362.01.T01:exon:24813;Parent=MsG0080048362.01.T01
contig283end exon 6636 6748 6636 ID=MsG0080048362.01.T01:exon:24814;Parent=MsG0080048362.01.T01
contig283end exon 6454 6541 6454 ID=MsG0080048362.01.T01:exon:24815;Parent=MsG0080048362.01.T01
contig283end exon 6091 6201 6091 ID=MsG0080048362.01.T01:exon:24816;Parent=MsG0080048362.01.T01
contig283end exon 5800 5982 5800 ID=MsG0080048362.01.T01:exon:24817;Parent=MsG0080048362.01.T01
contig283end exon 5369 5475 5369 ID=MsG0080048362.01.T01:exon:24818;Parent=MsG0080048362.01.T01
contig283end exon 5137 5275 5137 ID=MsG0080048362.01.T01:exon:24819;Parent=MsG0080048362.01.T01
contig283end CDS 8126 8336 8126 ID=MsG0080048362.01.T01:cds;Parent=MsG0080048362.01.T01
contig283end CDS 7197 7297 7197 ID=MsG0080048362.01.T01:cds;Parent=MsG0080048362.01.T01
contig283end CDS 6636 6748 6636 ID=MsG0080048362.01.T01:cds;Parent=MsG0080048362.01.T01
contig283end CDS 6454 6541 6454 ID=MsG0080048362.01.T01:cds;Parent=MsG0080048362.01.T01
contig283end CDS 6091 6201 6091 ID=MsG0080048362.01.T01:cds;Parent=MsG0080048362.01.T01
contig283end CDS 5800 5982 5800 ID=MsG0080048362.01.T01:cds;Parent=MsG0080048362.01.T01
contig283end CDS 5369 5475 5369 ID=MsG0080048362.01.T01:cds;Parent=MsG0080048362.01.T01
contig283end CDS 5137 5275 5137 ID=MsG0080048362.01.T01:cds;Parent=MsG0080048362.01.T01
Gene Sequence

>MsG0080048362.01.T01

ATGGCTAGCACCAGTGGGACAAAGGGTCCTTTTGAAATCTCTAGACAACCTTCAAAGAGGATGATAAGGGCACCTACAAGGACTGTTGAGTTGCCAAATGAAGAAAATATTATGGATAGTGAGATTGTTCCTTCTTCTCTTGCTGTTCTTGTGCCAATTCTTAGAGCAGCTATTGAGATTGAAGGGGAGAATCCAAGGGTTGCTTATCTTTGTCGCTTTCATGCATTTGAGAAGGCTCATACAATGGATCCAACATCAAGTGGGCGTGGTGTTCGTCAATTCAAGACTTACCTGTTGCACAAACTCGAAAAGGAAGGAGAGCTTACAGAGCAACATACTAAAAGAAGTGATGCGAGGGAGCTACAGAATTACTACCAATATTTCTACGAGAAGAGAATCCGGGATGGTGAATATACTAAAAAACCGGAGGAAATGGTTAGGAACGTTCAGATAGCAACTGTGTTGTATGAAGTACTGAGGACTTTGCTAACTCCACAAACTATTGAGGAGAAGACAAAAAGATATGCTGCAGATGTTGAGAATAAGAGGGGACAGTATGAGCATTACAATATTCTTCCATTATATGCTGTCGGTGTTAAACCAGTGATCATGGAACTTCCCGAGATCAAAGCTGCACTTGCTGCTTTAAGTAAAGTGGATAATCTCCCAATGCCTATCATTCATTCAAGACCTGATAATGATGATTCTACAATGCCTATGGAGAGGGTTAAAAATGTGAATGATATACTTGATTGGATTGCCTCCATTTTTGGATTTCAGGTAATGAATATTTCTCTTCTCTGTATAAAGAGTTTTTTGCAGATCCGTGAAGATACTATAGAGAAGTTGATGGCCACAACTTTCAAAAATTATGAGTCGTGGTGTCATTATGTGCGTTGTAAATCAAATATTAGGTATTCAGATGGTCAAGACAGACAACAATTAGAACTGATTTACATCGCACTTTATCTTCTAATATGGGGAGAAGCTTCAAATATTCGCTTTATGCCTGAATGTCTTTGCTATATTTTCCATCATGTAAGTTGTCCGTAA
Protein sequence

>MsG0080048362.01.T01

MASTSGTKGPFEISRQPSKRMIRAPTRTVELPNEENIMDSEIVPSSLAVLVPILRAAIEIEGENPRVAYLCRFHAFEKAHTMDPTSSGRGVRQFKTYLLHKLEKEGELTEQHTKRSDARELQNYYQYFYEKRIRDGEYTKKPEEMVRNVQIATVLYEVLRTLLTPQTIEEKTKRYAADVENKRGQYEHYNILPLYAVGVKPVIMELPEIKAALAALSKVDNLPMPIIHSRPDNDDSTMPMERVKNVNDILDWIASIFGFQVMNISLLCIKSFLQIREDTIEKLMATTFKNYESWCHYVRCKSNIRYSDGQDRQQLELIYIALYLLIWGEASNIRFMPECLCYIFHHVSCP*