Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048425.01 | MsG0080048426.01 | 0.957461 | 2.567060e-115 | 8.894792e-110 |
| MsG0080048425.01 | MsG0680033014.01 | 0.854741 | 1.005503e-61 | 2.268227e-58 |
| MsG0080048425.01 | MsG0880045851.01 | 0.803759 | 2.990094e-49 | 1.515078e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 10 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTAGTCCTAATGTTGATTA+TGG | 0.176691 | contig313end:+80942 | MsG0080048426.01.T03:CDS |
| CTTTGGGACTGTAGATTTAG+AGG | 0.298362 | contig313end:-81012 | MsG0080048426.01.T03:intergenic |
| AAGCATGGTCAAGAAGTCTT+TGG | 0.353291 | contig313end:-81029 | MsG0080048426.01.T03:intergenic |
| AGCATGGTCAAGAAGTCTTT+GGG | 0.422192 | contig313end:-81028 | MsG0080048426.01.T03:intergenic |
| CACAAACCATAATCAACATT+AGG | 0.450638 | contig313end:-80948 | MsG0080048426.01.T03:intergenic |
| ATCTTCTGCCTCAGATAAAG+AGG | 0.487203 | contig313end:-80976 | MsG0080048426.01.T03:intergenic |
| GAATGCAAAGAGAAGGGACG+TGG | 0.558645 | contig313end:+80900 | MsG0080048426.01.T03:CDS |
| TCATGTGGAATGCAAAGAGA+AGG | 0.568615 | contig313end:+80893 | MsG0080048426.01.T01:CDS |
| GTGTGTTGCCTCTTTATCTG+AGG | 0.572934 | contig313end:+80968 | MsG0080048426.01.T03:CDS |
| CCATAATCAACATTAGGACT+AGG | 0.583229 | contig313end:-80942 | MsG0080048426.01.T01:intergenic |
| ATATCAAGCTATCTCAAGCA+TGG | 0.614801 | contig313end:-81044 | MsG0080048426.01.T01:intergenic |
| CATGTGGAATGCAAAGAGAA+GGG | 0.628774 | contig313end:+80894 | MsG0080048426.01.T01:CDS |
| AATGCAAAGAGAAGGGACGT+GGG | 0.703464 | contig313end:+80901 | MsG0080048426.01.T03:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CACAAACCATAATCAACATT+AGG | - | contig313end:80951-80970 | MsG0080048426.01.T03:intergenic | 30.0% | |
| CCATAATCAACATTAGGACT+AGG | - | contig313end:80945-80964 | MsG0080048426.01.T03:intergenic | 35.0% | |
| ! | CCTAGTCCTAATGTTGATTA+TGG | + | contig313end:80942-80961 | MsG0080048426.01.T03:CDS | 35.0% |
| AAGCATGGTCAAGAAGTCTT+TGG | - | contig313end:81032-81051 | None:intergenic | 40.0% | |
| AGCATGGTCAAGAAGTCTTT+GGG | - | contig313end:81031-81050 | MsG0080048426.01.T03:intergenic | 40.0% | |
| ATCTTCTGCCTCAGATAAAG+AGG | - | contig313end:80979-80998 | MsG0080048426.01.T03:intergenic | 40.0% | |
| !! | CTTTGGGACTGTAGATTTAG+AGG | - | contig313end:81015-81034 | MsG0080048426.01.T03:intergenic | 40.0% |
| AATGCAAAGAGAAGGGACGT+GGG | + | contig313end:80901-80920 | MsG0080048426.01.T03:CDS | 45.0% | |
| GTGTGTTGCCTCTTTATCTG+AGG | + | contig313end:80968-80987 | MsG0080048426.01.T03:CDS | 45.0% | |
| GAATGCAAAGAGAAGGGACG+TGG | + | contig313end:80900-80919 | MsG0080048426.01.T03:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig313end | gene | 80895 | 81053 | 80895 | ID=MsG0080048425.01;Name=MsG0080048425.01 |
| contig313end | mRNA | 80895 | 81053 | 80895 | ID=MsG0080048425.01.T01;Parent=MsG0080048425.01;Name=MsG0080048425.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|52 |
| contig313end | exon | 80895 | 81053 | 80895 | ID=MsG0080048425.01.T01:exon:10305;Parent=MsG0080048425.01.T01 |
| contig313end | CDS | 80895 | 81053 | 80895 | ID=MsG0080048425.01.T01:cds;Parent=MsG0080048425.01.T01 |
| Gene Sequence |
| Protein sequence |
