AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680033014.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680033014.01.T01 MTR_6g047880 80.833 120 16 1 14 126 18 137 7.77e-66 196
MsG0680033014.01.T01 MTR_6g047900 76.923 117 23 3 14 126 18 134 2.17e-59 179
MsG0680033014.01.T01 MTR_7g029540 60.784 102 35 2 14 110 20 121 3.27e-38 125
MsG0680033014.01.T01 MTR_3g463570 49.558 113 50 3 14 120 19 130 2.49e-25 93.6
MsG0680033014.01.T01 MTR_3g067270 43.860 114 58 2 14 122 18 130 2.60e-25 93.2
MsG0680033014.01.T01 MTR_3g467750 43.860 114 58 2 14 122 18 130 2.60e-25 93.2
MsG0680033014.01.T01 MTR_3g463570 50.442 113 49 3 14 120 39 150 3.01e-25 94.0
MsG0680033014.01.T01 MTR_3g067280 42.105 114 60 2 14 122 18 130 4.06e-24 90.5
MsG0680033014.01.T01 MTR_7g056817 42.017 119 58 4 14 122 18 135 5.08e-23 87.4
MsG0680033014.01.T01 MTR_3g467760 40.367 109 45 1 14 122 18 106 9.76e-23 86.3
MsG0680033014.01.T01 MTR_6g036620 40.000 115 61 2 14 122 18 130 1.21e-22 86.7
MsG0680033014.01.T01 MTR_7g044980 46.535 101 44 4 15 109 18 114 7.95e-22 84.7
MsG0680033014.01.T01 MTR_3g067555 43.333 120 56 3 14 122 5 123 2.55e-21 82.8
MsG0680033014.01.T01 MTR_3g067540 42.500 120 57 3 14 122 18 136 5.53e-21 82.4
MsG0680033014.01.T01 MTR_3g067555 42.500 120 57 3 14 122 18 136 5.53e-21 82.4
MsG0680033014.01.T01 MTR_3g067535 43.220 118 55 3 14 122 18 132 9.47e-21 81.6
MsG0680033014.01.T01 MTR_7g056803 43.220 118 57 4 14 122 18 134 9.85e-21 81.6
MsG0680033014.01.T01 MTR_3g067437 39.474 114 59 3 14 119 18 129 2.47e-20 80.5
MsG0680033014.01.T01 MTR_3g067500 40.179 112 60 3 18 123 1 111 3.46e-20 79.7
MsG0680033014.01.T01 MTR_3g067550 42.241 116 55 3 18 122 1 115 6.88e-19 76.6
MsG0680033014.01.T01 MTR_3g067555 42.241 116 55 3 18 122 1 115 6.88e-19 76.6
MsG0680033014.01.T01 MTR_6g017150 53.731 67 30 1 57 122 67 133 9.10e-19 76.6
MsG0680033014.01.T01 MTR_1g019650 44.554 101 48 3 14 113 18 111 1.77e-18 75.1
MsG0680033014.01.T01 MTR_8g022400 38.462 117 62 5 11 119 16 130 1.98e-18 75.9
MsG0680033014.01.T01 MTR_3g436100 41.053 95 43 3 45 126 59 153 3.96e-18 75.5
MsG0680033014.01.T01 MTR_3g067510 39.200 125 60 5 6 122 15 131 5.51e-18 74.7
MsG0680033014.01.T01 MTR_3g067430 41.322 121 57 4 14 122 18 136 9.76e-18 73.9
MsG0680033014.01.T01 MTR_4g026590 37.097 124 66 3 11 122 16 139 1.42e-17 73.9
MsG0680033014.01.T01 MTR_7g044920 35.537 121 65 2 14 123 17 135 1.59e-17 73.9
MsG0680033014.01.T01 MTR_3g067580 38.793 116 63 4 14 122 26 140 1.85e-17 73.6
MsG0680033014.01.T01 MTR_3g067570 44.211 95 42 4 18 105 1 91 4.21e-17 71.2
MsG0680033014.01.T01 MTR_8g022430 36.441 118 68 3 15 125 19 136 1.96e-16 70.9
MsG0680033014.01.T01 MTR_8g022420 44.000 75 41 1 46 119 55 129 1.19e-15 68.6
MsG0680033014.01.T01 MTR_3g067510 38.462 117 56 5 14 122 18 126 1.25e-15 68.6
MsG0680033014.01.T01 MTR_6g017170 37.736 106 59 3 14 112 18 123 1.70e-15 67.8
MsG0680033014.01.T01 MTR_8g461020 37.374 99 54 3 12 104 17 113 7.65e-13 61.2
MsG0680033014.01.T02 MTR_6g047880 81.034 116 15 1 1 109 22 137 6.97e-63 187
MsG0680033014.01.T02 MTR_6g047900 77.876 113 21 3 1 109 22 134 5.99e-57 172
MsG0680033014.01.T02 MTR_7g029540 62.245 98 32 2 1 93 24 121 1.97e-36 120
MsG0680033014.01.T02 MTR_3g463570 50.459 109 47 3 1 103 23 130 1.09e-23 88.6
MsG0680033014.01.T02 MTR_3g067270 44.545 110 55 2 1 105 22 130 1.67e-23 88.2
MsG0680033014.01.T02 MTR_3g467750 44.545 110 55 2 1 105 22 130 1.67e-23 88.2
MsG0680033014.01.T02 MTR_3g463570 50.459 109 47 3 1 103 43 150 2.55e-23 88.2
MsG0680033014.01.T02 MTR_3g067280 42.727 110 57 2 1 105 22 130 2.52e-22 85.1
MsG0680033014.01.T02 MTR_7g056817 42.609 115 55 4 1 105 22 135 2.65e-21 82.4
MsG0680033014.01.T02 MTR_3g467760 51.351 74 34 1 32 105 35 106 3.05e-21 81.6
MsG0680033014.01.T02 MTR_6g036620 40.909 110 57 2 2 105 23 130 7.30e-21 81.3
MsG0680033014.01.T02 MTR_3g067500 40.179 112 60 3 1 106 1 111 2.09e-20 79.7
MsG0680033014.01.T02 MTR_7g044980 45.918 98 43 4 1 92 21 114 2.63e-20 80.1
MsG0680033014.01.T02 MTR_7g056803 44.737 114 53 4 1 105 22 134 4.47e-20 79.3
MsG0680033014.01.T02 MTR_3g067550 42.241 116 55 3 1 105 1 115 4.75e-19 76.3
MsG0680033014.01.T02 MTR_3g067555 42.241 116 55 3 1 105 1 115 4.75e-19 76.3
MsG0680033014.01.T02 MTR_3g067555 42.241 116 55 3 1 105 9 123 6.00e-19 76.3
MsG0680033014.01.T02 MTR_6g017150 53.731 67 30 1 40 105 67 133 6.21e-19 76.6
MsG0680033014.01.T02 MTR_3g067540 42.241 116 55 3 1 105 22 136 6.43e-19 76.6
MsG0680033014.01.T02 MTR_3g067555 42.241 116 55 3 1 105 22 136 6.43e-19 76.6
MsG0680033014.01.T02 MTR_3g067437 40.000 110 56 3 1 102 22 129 9.62e-19 75.9
MsG0680033014.01.T02 MTR_3g067535 42.982 114 53 3 1 105 22 132 1.27e-18 75.5
MsG0680033014.01.T02 MTR_3g436100 41.053 95 43 3 28 109 59 153 3.58e-18 75.1
MsG0680033014.01.T02 MTR_3g067570 44.211 95 42 4 1 88 1 91 2.93e-17 70.9
MsG0680033014.01.T02 MTR_3g067580 40.179 112 59 4 1 105 30 140 5.06e-17 71.6
MsG0680033014.01.T02 MTR_8g022400 40.000 90 52 2 14 102 42 130 1.35e-16 70.5
MsG0680033014.01.T02 MTR_8g022430 41.772 79 45 1 32 109 59 137 1.70e-16 70.1
MsG0680033014.01.T02 MTR_1g019650 44.330 97 46 3 1 96 22 111 2.49e-16 68.9
MsG0680033014.01.T02 MTR_8g022420 44.000 75 41 1 29 102 55 129 8.84e-16 68.6
MsG0680033014.01.T02 MTR_3g067430 51.667 60 29 0 46 105 77 136 9.43e-16 68.2
MsG0680033014.01.T02 MTR_6g017170 39.216 102 55 3 1 95 22 123 1.87e-15 67.4
MsG0680033014.01.T02 MTR_7g044920 35.043 117 63 2 1 106 21 135 2.23e-15 67.8
MsG0680033014.01.T02 MTR_3g067510 39.823 113 52 5 1 105 27 131 4.19e-15 66.6
MsG0680033014.01.T02 MTR_3g067510 39.823 113 52 5 1 105 22 126 4.59e-15 66.2
MsG0680033014.01.T02 MTR_4g026590 37.607 117 61 3 1 105 23 139 4.68e-15 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 27 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAGTCCTAATGTTGATTA+TGG 0.176691 6:+54784259 MsG0680033014.01.T01:CDS
AGCATGGTCAAGAAGTCTTT+TGG 0.276295 6:-54784346 None:intergenic
CACAACCTACACCGCATGTT+TGG 0.290947 6:-54784105 None:intergenic
ACATGAGATTGACAAAAGTT+AGG 0.309257 6:-54784193 None:intergenic
AGAAGCAAACCGTTGTTATC+TGG 0.377036 6:+54784063 MsG0680033014.01.T01:CDS
ACAACCTACACCGCATGTTT+GGG 0.389533 6:-54784104 None:intergenic
CATGAGATTGACAAAAGTTA+GGG 0.391241 6:-54784192 None:intergenic
TTGCTTCTACTTTCTTCATC+GGG 0.430575 6:-54784048 None:intergenic
CACAAACCATAATCAACATT+AGG 0.450638 6:-54784265 None:intergenic
ACGAGATCTTTATATGATGC+AGG 0.464587 6:-54784151 None:intergenic
TTTGCTTCTACTTTCTTCAT+CGG 0.487039 6:-54784049 None:intergenic
ATCTTCTGCCTCAGATAAAG+AGG 0.487203 6:-54784293 None:intergenic
GGTGTAGGTTGTGTTTGTTT+CGG 0.512311 6:+54784115 MsG0680033014.01.T01:CDS
AAAGATCTCGTAAAGATACC+TGG 0.512553 6:+54784163 MsG0680033014.01.T01:CDS
GAATGCAAAGAGAAGGGACG+TGG 0.558645 6:+54784217 MsG0680033014.01.T01:CDS
TCATGTGGAATGCAAAGAGA+AGG 0.561795 6:+54784210 MsG0680033014.01.T01:CDS
GATCTTTATATGATGCAGGT+TGG 0.567760 6:-54784147 None:intergenic
GTGTGTTGCCTCTTTATCTG+AGG 0.572934 6:+54784285 MsG0680033014.01.T01:CDS
ACATCCCAAACATGCGGTGT+AGG 0.582167 6:+54784100 MsG0680033014.01.T01:CDS
CCATAATCAACATTAGGACT+AGG 0.583229 6:-54784259 None:intergenic
AAGATCTCGTAAAGATACCT+GGG 0.611149 6:+54784164 MsG0680033014.01.T01:CDS
ATATCAAGCTATCTCAAGCA+TGG 0.614801 6:-54784362 None:intergenic
CATGTGGAATGCAAAGAGAA+GGG 0.618807 6:+54784211 MsG0680033014.01.T01:CDS
AGGTTGTGTTTGTTTCGGTG+AGG 0.634590 6:+54784120 MsG0680033014.01.T01:CDS
AATGCAAAGAGAAGGGACGT+GGG 0.703464 6:+54784218 MsG0680033014.01.T01:CDS
TCAGAGACATCCCAAACATG+CGG 0.712944 6:+54784094 MsG0680033014.01.T01:CDS
AACAAACAGCCAGATAACAA+CGG 0.721899 6:-54784072 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ACATGAGATTGACAAAAGTT+AGG - Chr6:54784196-54784215 None:intergenic 30.0%
CACAAACCATAATCAACATT+AGG - Chr6:54784268-54784287 None:intergenic 30.0%
CATGAGATTGACAAAAGTTA+GGG - Chr6:54784195-54784214 None:intergenic 30.0%
TTTGCTTCTACTTTCTTCAT+CGG - Chr6:54784052-54784071 None:intergenic 30.0%
! TAACTTTTGTCAATCTCATG+TGG + Chr6:54784195-54784214 MsG0680033014.01.T01:CDS 30.0%
AAAGATCTCGTAAAGATACC+TGG + Chr6:54784163-54784182 MsG0680033014.01.T01:CDS 35.0%
AACAAACAGCCAGATAACAA+CGG - Chr6:54784075-54784094 None:intergenic 35.0%
AAGATCTCGTAAAGATACCT+GGG + Chr6:54784164-54784183 MsG0680033014.01.T01:CDS 35.0%
ACGAGATCTTTATATGATGC+AGG - Chr6:54784154-54784173 None:intergenic 35.0%
CCATAATCAACATTAGGACT+AGG - Chr6:54784262-54784281 None:intergenic 35.0%
GATCTTTATATGATGCAGGT+TGG - Chr6:54784150-54784169 None:intergenic 35.0%
TTGCTTCTACTTTCTTCATC+GGG - Chr6:54784051-54784070 None:intergenic 35.0%
! CCTAGTCCTAATGTTGATTA+TGG + Chr6:54784259-54784278 MsG0680033014.01.T01:CDS 35.0%
!!! TTTTGGGACTGTAGATTTAG+AGG - Chr6:54784332-54784351 None:intergenic 35.0%
AGAAGCAAACCGTTGTTATC+TGG + Chr6:54784063-54784082 MsG0680033014.01.T01:CDS 40.0%
ATCTTCTGCCTCAGATAAAG+AGG - Chr6:54784296-54784315 None:intergenic 40.0%
CATGTGGAATGCAAAGAGAA+GGG + Chr6:54784211-54784230 MsG0680033014.01.T01:CDS 40.0%
GGTGTAGGTTGTGTTTGTTT+CGG + Chr6:54784115-54784134 MsG0680033014.01.T01:CDS 40.0%
TCATGTGGAATGCAAAGAGA+AGG + Chr6:54784210-54784229 MsG0680033014.01.T01:CDS 40.0%
! AGCATGGTCAAGAAGTCTTT+TGG - Chr6:54784349-54784368 None:intergenic 40.0%
! CAAAAGTTAGGGTTTTTCCC+AGG - Chr6:54784184-54784203 None:intergenic 40.0%
! GCATGGTCAAGAAGTCTTTT+GGG - Chr6:54784348-54784367 None:intergenic 40.0%
AATGCAAAGAGAAGGGACGT+GGG + Chr6:54784218-54784237 MsG0680033014.01.T01:CDS 45.0%
ACAACCTACACCGCATGTTT+GGG - Chr6:54784107-54784126 None:intergenic 45.0%
AGGTTGTGTTTGTTTCGGTG+AGG + Chr6:54784120-54784139 MsG0680033014.01.T01:CDS 45.0%
GTGTGTTGCCTCTTTATCTG+AGG + Chr6:54784285-54784304 MsG0680033014.01.T01:CDS 45.0%
TCAGAGACATCCCAAACATG+CGG + Chr6:54784094-54784113 MsG0680033014.01.T01:CDS 45.0%
ACATCCCAAACATGCGGTGT+AGG + Chr6:54784100-54784119 MsG0680033014.01.T01:CDS 50.0%
CACAACCTACACCGCATGTT+TGG - Chr6:54784108-54784127 None:intergenic 50.0%
GAATGCAAAGAGAAGGGACG+TGG + Chr6:54784217-54784236 MsG0680033014.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr6 gene 54784001 54784381 54784001 ID=MsG0680033014.01;Name=MsG0680033014.01
Chr6 mRNA 54784001 54784381 54784001 ID=MsG0680033014.01.T01;Parent=MsG0680033014.01;Name=MsG0680033014.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|126
Chr6 exon 54784001 54784381 54784001 ID=MsG0680033014.01.T01:exon:9200;Parent=MsG0680033014.01.T01
Chr6 CDS 54784001 54784381 54784001 ID=MsG0680033014.01.T01:cds;Parent=MsG0680033014.01.T01
Chr6 mRNA 54784052 54784381 54784052 ID=MsG0680033014.01.T02;Parent=MsG0680033014.01;Name=MsG0680033014.01.T02;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|109
Chr6 exon 54784052 54784381 54784052 ID=MsG0680033014.01.T02:exon:9201;Parent=MsG0680033014.01.T02
Chr6 CDS 54784052 54784381 54784052 ID=MsG0680033014.01.T02:cds;Parent=MsG0680033014.01.T02
Chr6 mRNA 54784212 54784361 54784212 ID=MsG0680033014.01.T03;Parent=MsG0680033014.01;Name=MsG0680033014.01.T03;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|49
Chr6 exon 54784212 54784361 54784212 ID=MsG0680033014.01.T03:exon:9202;Parent=MsG0680033014.01.T03
Chr6 CDS 54784212 54784361 54784212 ID=MsG0680033014.01.T03:cds;Parent=MsG0680033014.01.T03
Gene Sequence

>MsG0680033014.01.T01

ATGAAAGAGTTTCATTCTAAAATATCAACTTCATTTGCAGTAATTCTCCCGATGAAGAAAGTAGAAGCAAACCGTTGTTATCTGGCTGTTTGTTCAGAGACATCCCAAACATGCGGTGTAGGTTGTGTTTGTTTCGGTGAGGATTGCCAACCTGCATCATATAAAGATCTCGTAAAGATACCTGGGAAAAACCCTAACTTTTGTCAATCTCATGTGGAATGCAAAGAGAAGGGACGTGGGAGCTTCTGCGCTCGTCATCCTAGTCCTAATGTTGATTATGGTTTGTGTGTTGCCTCTTTATCTGAGGCAGAAGATTTCTTCAAGATTGCCTCTAAATCTACAGTCCCAAAAGACTTCTTGACCATGCTTGAGATAGCTTGA

>MsG0680033014.01.T02

ATGAAGAAAGTAGAAGCAAACCGTTGTTATCTGGCTGTTTGTTCAGAGACATCCCAAACATGCGGTGTAGGTTGTGTTTGTTTCGGTGAGGATTGCCAACCTGCATCATATAAAGATCTCGTAAAGATACCTGGGAAAAACCCTAACTTTTGTCAATCTCATGTGGAATGCAAAGAGAAGGGACGTGGGAGCTTCTGCGCTCGTCATCCTAGTCCTAATGTTGATTATGGTTTGTGTGTTGCCTCTTTATCTGAGGCAGAAGATTTCTTCAAGATTGCCTCTAAATCTACAGTCCCAAAAGACTTCTTGACCATGCTTGAGATAGCTTGA

>MsG0680033014.01.T03

ATGTGGAATGCAAAGAGAAGGGACGTGGGAGCTTCTGCGCTCGTCATCCTAGTCCTAATGTTGATTATGGTTTGTGTGTTGCCTCTTTATCTGAGGCAGAAGATTTCTTCAAGATTGCCTCTAAATCTACAGTCCCAAAAGACTTCTTGA

Protein sequence

>MsG0680033014.01.T01

MKEFHSKISTSFAVILPMKKVEANRCYLAVCSETSQTCGVGCVCFGEDCQPASYKDLVKIPGKNPNFCQSHVECKEKGRGSFCARHPSPNVDYGLCVASLSEAEDFFKIASKSTVPKDFLTMLEIA*

>MsG0680033014.01.T02

MKKVEANRCYLAVCSETSQTCGVGCVCFGEDCQPASYKDLVKIPGKNPNFCQSHVECKEKGRGSFCARHPSPNVDYGLCVASLSEAEDFFKIASKSTVPKDFLTMLEIA*

>MsG0680033014.01.T03

MWNAKRRDVGASALVILVLMLIMVCVLPLYLRQKISSRLPLNLQSQKTS*