AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048897.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048897.01.T01 MTR_8g099415 96.581 585 20 0 5 589 2 586 0.0 1144
MsG0080048897.01.T01 MTR_8g099415 96.370 551 20 0 5 555 2 552 0.0 1080
MsG0080048897.01.T01 MTR_0050s0150 55.340 103 21 3 299 400 48 126 3.50e-21 90.1
MsG0080048897.01.T01 MTR_4g116170 26.961 408 262 10 70 460 186 574 3.44e-19 91.7
MsG0080048897.01.T01 MTR_4g116170 25.502 498 273 14 6 470 53 485 6.33e-18 87.8
MsG0080048897.01.T01 MTR_4g009870 28.753 393 226 11 82 460 26 378 3.66e-18 87.0
MsG0080048897.01.T01 MTR_4g009870 25.500 400 260 8 81 475 1 367 1.01e-16 82.8
MsG0080048897.01.T01 MTR_4g009870 27.900 319 164 8 174 485 45 304 1.84e-13 72.8
MsG0080048897.01.T01 MTR_4g009870 25.130 386 201 9 82 460 26 330 3.93e-12 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048897.01.T01 AT4G23840 50.347 576 279 5 4 572 2 577 1.18e-164 483
MsG0080048897.01.T01 AT1G15740 25.840 476 310 16 6 475 61 499 8.08e-20 94.0

Find 8 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAATACTTGAGAAGCAAT+TGG 0.394370 contig521end:+13390 MsG0080048897.01.T01:exon
TGAGTTGTCTATTTCACTAC+TGG 0.394807 contig521end:-13371 MsG0080048897.01.T01:intergenic
AAATAAATTACCAATCCAAT+GGG 0.504668 contig521end:-13451 MsG0080048897.01.T01:intergenic
AAAATAAATTACCAATCCAA+TGG 0.522277 contig521end:-13452 MsG0080048897.01.T01:intergenic
ACTATCTGCTCCCATTGGAT+TGG 0.537413 contig521end:+13441 MsG0080048897.01.T01:exon
ATGTAACTATCTGCTCCCAT+TGG 0.579966 contig521end:+13436 MsG0080048897.01.T01:exon
GTTGTCTATTTCACTACTGG+AGG 0.610400 contig521end:-13368 MsG0080048896.01.T01:intergenic
AATGGGAGCAGATAGTTACA+TGG 0.633046 contig521end:-13434 MsG0080048896.01.T01:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACAATAAAATTTGATAT+TGG - contig521end:10794-10813 MsG0080048897.01.T01:intergenic 10.0%
!! TGTTTAATGAATTAATTATT+TGG + contig521end:12463-12482 MsG0080048897.01.T01:intron 10.0%
!! AAAATCTTAATCAATTACAA+AGG + contig521end:12770-12789 MsG0080048897.01.T01:CDS 15.0%
!! AACATTAAGATTTCTTAATA+TGG - contig521end:12294-12313 MsG0080048897.01.T01:intergenic 15.0%
!!! TAATTGATTAAGATTTTGTA+GGG - contig521end:12767-12786 MsG0080048897.01.T01:intergenic 15.0%
!!! TGTTTTAATTTAACATTGAT+TGG + contig521end:11954-11973 MsG0080048897.01.T01:intron 15.0%
!! AAAATAAATTACCAATCCAA+TGG - contig521end:13455-13474 MsG0080048897.01.T01:intergenic 20.0%
!! AAACAAAAATCAAATGGAAA+TGG - contig521end:10054-10073 MsG0080048897.01.T01:intergenic 20.0%
!! AAATAAATTACCAATCCAAT+GGG - contig521end:13454-13473 MsG0080048897.01.T01:intergenic 20.0%
!! AACTTGAATTAAATGCTTAA+TGG - contig521end:12523-12542 MsG0080048897.01.T01:intergenic 20.0%
!! AATTAAAACAAAGATATAGC+TGG - contig521end:11944-11963 MsG0080048897.01.T01:intergenic 20.0%
!! ACTTGAATTAAATGCTTAAT+GGG - contig521end:12522-12541 MsG0080048897.01.T01:intergenic 20.0%
!! ATATGTATTTGCATACAATA+AGG - contig521end:11363-11382 MsG0080048897.01.T01:intergenic 20.0%
!! TGTGATTTATGAAATGAAAT+TGG - contig521end:10096-10115 MsG0080048897.01.T01:intergenic 20.0%
!!! ATTAAAGGTATTTGATTACA+TGG + contig521end:11704-11723 MsG0080048897.01.T01:intron 20.0%
!!! CGTAGTTTTTTTTTTTTTTC+AGG + contig521end:10966-10985 MsG0080048897.01.T01:intron 20.0%
!!! GCTGTCTTTTTTTTTTTTAA+TGG - contig521end:12249-12268 MsG0080048897.01.T01:intergenic 20.0%
!!! GTAATTGATTAAGATTTTGT+AGG - contig521end:12768-12787 MsG0080048897.01.T01:intergenic 20.0%
!!! TTTATTTTGACATAGATCAA+AGG + contig521end:13290-13309 MsG0080048897.01.T01:intron 20.0%
! AAATCAAATGGAAATGGAAA+AGG - contig521end:10048-10067 MsG0080048897.01.T01:intergenic 25.0%
! AAGAGGAAACAAAAATCAAA+TGG - contig521end:10060-10079 MsG0080048897.01.T01:intergenic 25.0%
! AATTCCAGCATCATTAAATT+TGG - contig521end:10500-10519 MsG0080048897.01.T01:intergenic 25.0%
! AGTGAAAGAATTTCAAGATT+GGG - contig521end:11599-11618 MsG0080048897.01.T01:intergenic 25.0%
! CAAAAGATTAGAAACGTAAT+GGG - contig521end:13229-13248 MsG0080048897.01.T01:intergenic 25.0%
! CTATATAAGAAACTTAAGTG+GGG - contig521end:12008-12027 MsG0080048897.01.T01:intergenic 25.0%
! GCTATAGTAACAGAAAAATT+TGG - contig521end:11901-11920 MsG0080048897.01.T01:intergenic 25.0%
! GCTATATAAGAAACTTAAGT+GGG - contig521end:12009-12028 MsG0080048897.01.T01:intergenic 25.0%
! TAAAGAAAAAGACTCATCAA+CGG - contig521end:11774-11793 MsG0080048897.01.T01:intergenic 25.0%
! TAGTGAAAGAATTTCAAGAT+TGG - contig521end:11600-11619 MsG0080048897.01.T01:intergenic 25.0%
! TCAATTACAAAGGTTGAATT+TGG + contig521end:12780-12799 MsG0080048897.01.T01:CDS 25.0%
! TCGATCTCAATCAAAATAAA+CGG - contig521end:10724-10743 MsG0080048897.01.T01:intergenic 25.0%
! TGCTATATAAGAAACTTAAG+TGG - contig521end:12010-12029 MsG0080048897.01.T01:intergenic 25.0%
! TGTTCAAAATGTCTAAATAC+TGG + contig521end:12586-12605 MsG0080048897.01.T01:intron 25.0%
! TGTTTCAATTAGACAAATTC+TGG + contig521end:12546-12565 MsG0080048897.01.T01:intron 25.0%
! TTCTGTAATTATGGATTTCT+AGG - contig521end:12206-12225 MsG0080048897.01.T01:intergenic 25.0%
! TTGTAATCTGTTACTCAATT+GGG + contig521end:10831-10850 MsG0080048897.01.T01:intron 25.0%
!! ATCTTTGAGAATAGAATCAA+TGG - contig521end:11279-11298 MsG0080048897.01.T01:intergenic 25.0%
!! ATTGATTCTATTCTCAAAGA+TGG + contig521end:11278-11297 MsG0080048897.01.T01:CDS 25.0%
!! CGTTGTATTTTCAAATATTC+TGG + contig521end:13487-13506 MsG0080048897.01.T01:exon 25.0%
!!! CTTCATTGTTTGTTTTAGTT+AGG + contig521end:10934-10953 MsG0080048897.01.T01:intron 25.0%
AAATGGAAATGGAAAAGGAA+AGG - contig521end:10043-10062 MsG0080048897.01.T01:intergenic 30.0%
AAGAGATAAGTGTTTAAGAG+AGG - contig521end:10605-10624 MsG0080048897.01.T01:intergenic 30.0%
AATGGAAATGGAAAAGGAAA+GGG - contig521end:10042-10061 MsG0080048897.01.T01:intergenic 30.0%
ACACAATCACAGTTCAAAAA+CGG - contig521end:10869-10888 MsG0080048897.01.T01:intergenic 30.0%
ACAGATCTGGAAGTTTAATT+TGG + contig521end:11980-11999 MsG0080048897.01.T01:intron 30.0%
ACCCGTATTAGACTATAATA+TGG + contig521end:12351-12370 MsG0080048897.01.T01:intron 30.0%
ACCTACAAAATTTGGAGATA+AGG - contig521end:13202-13221 MsG0080048897.01.T01:intergenic 30.0%
AGCACAGAAATTTACTATTG+AGG - contig521end:11810-11829 MsG0080048897.01.T01:intergenic 30.0%
AGTAACAGATTACAACTAAC+TGG - contig521end:10826-10845 MsG0080048897.01.T01:intergenic 30.0%
AGTCCAATCAAGATTTAGAT+GGG - contig521end:11198-11217 MsG0080048897.01.T01:intergenic 30.0%
ATATTAAGATAGTACTGCTG+AGG + contig521end:12320-12339 MsG0080048897.01.T01:intron 30.0%
ATCCTAAACTTTGTCAACTT+AGG - contig521end:12925-12944 MsG0080048897.01.T01:intergenic 30.0%
ATGAAGTCTAACAGTGAATT+CGG - contig521end:10920-10939 MsG0080048897.01.T01:intergenic 30.0%
ATTAACAACACAGACTTGTA+TGG - contig521end:11028-11047 MsG0080048897.01.T01:intergenic 30.0%
CAAGGTTCAAATAACGTAAA+TGG - contig521end:10393-10412 MsG0080048897.01.T01:intergenic 30.0%
CACTAAAAAAGATGAATAGC+TGG - contig521end:12052-12071 MsG0080048897.01.T01:intergenic 30.0%
CATCATGCTGATATATAAGA+GGG - contig521end:11648-11667 MsG0080048897.01.T01:intergenic 30.0%
CATTCAAGAGATGTTAAATC+AGG - contig521end:11236-11255 MsG0080048897.01.T01:intergenic 30.0%
CCAAAAGATTAGAAACGTAA+TGG - contig521end:13230-13249 MsG0080048897.01.T01:intergenic 30.0%
CCCATATTATAGTCTAATAC+GGG - contig521end:12355-12374 MsG0080048897.01.T01:intergenic 30.0%
CCCCATATTATAGTCTAATA+CGG - contig521end:12356-12375 MsG0080048897.01.T01:intergenic 30.0%
CCCGTATTAGACTATAATAT+GGG + contig521end:12352-12371 MsG0080048897.01.T01:intron 30.0%
CCGTATTAGACTATAATATG+GGG + contig521end:12353-12372 MsG0080048897.01.T01:intron 30.0%
CTGAGCAATACAAACATTAA+AGG + contig521end:11689-11708 MsG0080048897.01.T01:CDS 30.0%
GACAGTAAAATATCAAACCA+AGG + contig521end:11140-11159 MsG0080048897.01.T01:CDS 30.0%
GAGATAAATATCTCAGTTCT+TGG - contig521end:12852-12871 MsG0080048897.01.T01:intergenic 30.0%
GTTGTAATCTGTTACTCAAT+TGG + contig521end:10830-10849 MsG0080048897.01.T01:intron 30.0%
TAAAGGGAATAAAGCTTCAT+CGG - contig521end:12821-12840 MsG0080048897.01.T01:intergenic 30.0%
TACAAAATTTGGAGATAAGG+AGG - contig521end:13199-13218 MsG0080048897.01.T01:intergenic 30.0%
TAGATAGATCCAAGTCTTTA+AGG - contig521end:10471-10490 MsG0080048897.01.T01:intergenic 30.0%
TAGTCCAATCAAGATTTAGA+TGG - contig521end:11199-11218 MsG0080048897.01.T01:intergenic 30.0%
TCTCTAAATTAGGAACAGAA+AGG - contig521end:10531-10550 MsG0080048897.01.T01:intergenic 30.0%
TGGGAATTACCTACAAAATT+TGG - contig521end:13210-13229 MsG0080048897.01.T01:intergenic 30.0%
TTAGACTATAATATGGGGTT+TGG + contig521end:12358-12377 MsG0080048897.01.T01:intron 30.0%
TTGAAAAGTTTGAATCTGAG+TGG + contig521end:11527-11546 MsG0080048897.01.T01:CDS 30.0%
TTGAATAAAAGCCACAACAT+GGG - contig521end:12115-12134 MsG0080048897.01.T01:intergenic 30.0%
TTGGAATTTAGAAGAAGAAG+AGG - contig521end:10077-10096 MsG0080048897.01.T01:intergenic 30.0%
! AAAGTTTAGGATTTGTGATG+CGG + contig521end:12933-12952 MsG0080048897.01.T01:CDS 30.0%
! AACTTGATTTCTCTCAGTTT+TGG + contig521end:12620-12639 MsG0080048897.01.T01:intron 30.0%
! AATGAATAGCCTTAAAGACT+TGG + contig521end:10459-10478 MsG0080048897.01.T01:CDS 30.0%
! ATGAGTCTTTTTCTTTACCA+CGG + contig521end:11777-11796 MsG0080048897.01.T01:intron 30.0%
! CCATTACGTTTCTAATCTTT+TGG + contig521end:13227-13246 MsG0080048897.01.T01:intron 30.0%
! GAGAAAATAAGCTTTGTCAA+AGG - contig521end:11311-11330 MsG0080048897.01.T01:intergenic 30.0%
! TAGACAAAGTACTATACTAC+AGG - contig521end:12667-12686 MsG0080048897.01.T01:intergenic 30.0%
! TCCTTATCTCCAAATTTTGT+AGG + contig521end:13198-13217 MsG0080048897.01.T01:CDS 30.0%
! TGAAGTCCATTTTCTTCAAA+AGG - contig521end:13114-13133 MsG0080048897.01.T01:intergenic 30.0%
! TTCCTAAGTTGACAAAGTTT+AGG + contig521end:12920-12939 MsG0080048897.01.T01:CDS 30.0%
! TTGATTAAGATTTTGTAGGG+CGG - contig521end:12764-12783 MsG0080048897.01.T01:intergenic 30.0%
!! GTACTTCCTTTTGAAGAAAA+TGG + contig521end:13105-13124 MsG0080048897.01.T01:CDS 30.0%
!! TGACAAAGCTTATTTTCTCT+GGG + contig521end:11312-11331 MsG0080048897.01.T01:CDS 30.0%
!! TTGACAAAGCTTATTTTCTC+TGG + contig521end:11311-11330 MsG0080048897.01.T01:CDS 30.0%
!!! CGCTTTTTCTTCTGTAATTA+TGG - contig521end:12215-12234 MsG0080048897.01.T01:intergenic 30.0%
!!! TCGTTGCAGGTATATTTTTT+GGG + contig521end:10667-10686 MsG0080048897.01.T01:intron 30.0%
!!! TTCGTTGCAGGTATATTTTT+TGG + contig521end:10666-10685 MsG0080048897.01.T01:intron 30.0%
AAAAAAAAGACAGCCACACT+AGG + contig521end:12255-12274 MsG0080048897.01.T01:intron 35.0%
AAATGGAAAAGGAAAGGGAA+AGG - contig521end:10037-10056 MsG0080048897.01.T01:intergenic 35.0%
AAGATTTAGATGGGTCAACT+TGG - contig521end:11189-11208 MsG0080048897.01.T01:intergenic 35.0%
ACACTTATCTCTTCTTGACT+TGG + contig521end:10612-10631 MsG0080048897.01.T01:CDS 35.0%
ACTTAGGAACTGATGATAGA+TGG - contig521end:12909-12928 MsG0080048897.01.T01:intergenic 35.0%
AGATCAACATCCTTCAATGA+AGG - contig521end:11671-11690 MsG0080048897.01.T01:intergenic 35.0%
AGATTTAGATGGGTCAACTT+GGG - contig521end:11188-11207 MsG0080048897.01.T01:intergenic 35.0%
AGGCTATTCATTCCTGAAAT+AGG - contig521end:10451-10470 MsG0080048897.01.T01:intergenic 35.0%
AGTTCTTGGAAGCTTGATAA+AGG - contig521end:12838-12857 MsG0080048897.01.T01:intergenic 35.0%
ATAAGGTTTCAGCTTCATTC+AGG - contig521end:11346-11365 MsG0080048897.01.T01:intergenic 35.0%
ATCACCCACAAATTGAAGTA+AGG + contig521end:13067-13086 MsG0080048897.01.T01:CDS 35.0%
ATCTACAACACATTGAACTC+TGG + contig521end:11117-11136 MsG0080048897.01.T01:CDS 35.0%
ATCTGTTACTCAATTGGGTT+TGG + contig521end:10836-10855 MsG0080048897.01.T01:intron 35.0%
ATGCGCAACTTCTCTAAATT+AGG - contig521end:10541-10560 MsG0080048897.01.T01:intergenic 35.0%
CACTTATCTCTTCTTGACTT+GGG + contig521end:10613-10632 MsG0080048897.01.T01:CDS 35.0%
CATGCCTTACTTCAATTTGT+GGG - contig521end:13074-13093 MsG0080048897.01.T01:intergenic 35.0%
CTCAATACTTGAGAAGCAAT+TGG + contig521end:13390-13409 MsG0080048897.01.T01:exon 35.0%
CTTGAATAAAAGCCACAACA+TGG - contig521end:12116-12135 MsG0080048897.01.T01:intergenic 35.0%
GAATCAATTCATCTGCTCTT+TGG + contig521end:10425-10444 MsG0080048897.01.T01:CDS 35.0%
GCATCATGCTGATATATAAG+AGG - contig521end:11649-11668 MsG0080048897.01.T01:intergenic 35.0%
GTTCTTGGAAGCTTGATAAA+GGG - contig521end:12837-12856 MsG0080048897.01.T01:intergenic 35.0%
TACATATTTCCTGTCCTTTC+TGG + contig521end:11376-11395 MsG0080048897.01.T01:CDS 35.0%
TAGATGGTAAAGTGAGATGT+CGG - contig521end:12893-12912 MsG0080048897.01.T01:intergenic 35.0%
TATTAAGAATAGCAGCACCT+TGG - contig521end:11160-11179 MsG0080048897.01.T01:intergenic 35.0%
TCATGCCTTACTTCAATTTG+TGG - contig521end:13075-13094 MsG0080048897.01.T01:intergenic 35.0%
TCCATCAGTTTAAGAGTTTC+AGG - contig521end:12994-13013 MsG0080048897.01.T01:intergenic 35.0%
TCCTGAAACTCTTAAACTGA+TGG + contig521end:12990-13009 MsG0080048897.01.T01:CDS 35.0%
TCTACAACACATTGAACTCT+GGG + contig521end:11118-11137 MsG0080048897.01.T01:CDS 35.0%
TGACCCATCTAAATCTTGAT+TGG + contig521end:11192-11211 MsG0080048897.01.T01:CDS 35.0%
TGAGTTGTCTATTTCACTAC+TGG - contig521end:13374-13393 MsG0080048897.01.T01:intergenic 35.0%
TGCTCCAAATTTAATGATGC+TGG + contig521end:10493-10512 MsG0080048897.01.T01:CDS 35.0%
TTCTCCTGACATATTAATGC+AGG - contig521end:12078-12097 MsG0080048897.01.T01:intergenic 35.0%
TTTGTGTTCTGCATTGTTCA+TGG + contig521end:10696-10715 MsG0080048897.01.T01:intron 35.0%
! ATGTAGTGGTCTTGAATTAC+TGG + contig521end:10894-10913 MsG0080048897.01.T01:intron 35.0%
! TGTGTATGTTGTTGGTTACT+TGG + contig521end:10991-11010 MsG0080048897.01.T01:intron 35.0%
!! GTCTTTGAAGTTAATCGAGA+TGG - contig521end:13147-13166 MsG0080048897.01.T01:intergenic 35.0%
!! TGATTGTGTTGCTTATGTAG+TGG + contig521end:10880-10899 MsG0080048897.01.T01:intron 35.0%
AAACTCATTTGCCCATGTTG+TGG + contig521end:12101-12120 MsG0080048897.01.T01:intron 40.0%
AATCCAAATCAGAACTTCCC+TGG - contig521end:12729-12748 MsG0080048897.01.T01:intergenic 40.0%
AATGGGAGCAGATAGTTACA+TGG - contig521end:13437-13456 MsG0080048897.01.T01:intergenic 40.0%
AATGTGCAAACACCCAACAA+AGG - contig521end:12160-12179 MsG0080048897.01.T01:intergenic 40.0%
ACAAATGGCGGTTACAAAGA+CGG + contig521end:10194-10213 MsG0080048897.01.T01:CDS 40.0%
AGCTAAAGATGCATCATCGA+CGG - contig521end:10647-10666 MsG0080048897.01.T01:intergenic 40.0%
ATGTAACTATCTGCTCCCAT+TGG + contig521end:13436-13455 MsG0080048897.01.T01:exon 40.0%
CAATCTCAGCTCATGTATGA+TGG + contig521end:11463-11482 MsG0080048897.01.T01:CDS 40.0%
CACTGTGTTTGAAAACTTCG+AGG - contig521end:10300-10319 MsG0080048897.01.T01:intergenic 40.0%
GATGAAGAGCGAAATCTCAT+TGG + contig521end:10126-10145 MsG0080048897.01.T01:exon 40.0%
GATTTAGATGGGTCAACTTG+GGG - contig521end:11187-11206 MsG0080048897.01.T01:intergenic 40.0%
GCAGAATGGATGGCATATTT+AGG + contig521end:10358-10377 MsG0080048897.01.T01:CDS 40.0%
GGAATCAAGTGACCTTTGTT+GGG + contig521end:12145-12164 MsG0080048897.01.T01:intron 40.0%
GGATGATGATTCAGTTGAGA+TGG + contig521end:11484-11503 MsG0080048897.01.T01:CDS 40.0%
GTGTCCTGCATTAATATGTC+AGG + contig521end:12071-12090 MsG0080048897.01.T01:intron 40.0%
GTTGAGATGGTTGCATGTAT+TGG + contig521end:11497-11516 MsG0080048897.01.T01:CDS 40.0%
GTTGTCTATTTCACTACTGG+AGG - contig521end:13371-13390 MsG0080048897.01.T01:intergenic 40.0%
GTTTGAATCTGAGTGGTACT+TGG + contig521end:11534-11553 MsG0080048897.01.T01:CDS 40.0%
TGAAAACTTCGAGGAGAGAT+GGG - contig521end:10291-10310 MsG0080048897.01.T01:intergenic 40.0%
TGATTCGATGACATTCAGCA+AGG - contig521end:10411-10430 MsG0080048897.01.T01:intergenic 40.0%
TGCTGATGAATATTCTCGTG+AGG + contig521end:11866-11885 MsG0080048897.01.T01:intron 40.0%
TGGAATCAAGTGACCTTTGT+TGG + contig521end:12144-12163 MsG0080048897.01.T01:intron 40.0%
TGGTGCAATTACTCATGTTG+AGG - contig521end:11259-11278 MsG0080048897.01.T01:intergenic 40.0%
TTACAACTAACTGGCAACAC+TGG - contig521end:10817-10836 MsG0080048897.01.T01:intergenic 40.0%
TTGAAAACTTCGAGGAGAGA+TGG - contig521end:10292-10311 MsG0080048897.01.T01:intergenic 40.0%
TTGTAACCGCCATTTGTCAA+CGG - contig521end:10191-10210 MsG0080048897.01.T01:intergenic 40.0%
TTTCAAACACAGTGTGGAAG+AGG + contig521end:10306-10325 MsG0080048897.01.T01:CDS 40.0%
! AAAACCAGTGTCACTACCAA+AGG + contig521end:10565-10584 MsG0080048897.01.T01:CDS 40.0%
! CGAAGTTTTCAAACACAGTG+TGG + contig521end:10300-10319 MsG0080048897.01.T01:CDS 40.0%
! GTTGTGGCTTTTATTCAAGC+TGG + contig521end:12117-12136 MsG0080048897.01.T01:intron 40.0%
! TTCAGGAGTGTGTATGTTGT+TGG + contig521end:10983-11002 MsG0080048897.01.T01:intron 40.0%
!! TGATTTCTCTCAGTTTTGGC+TGG + contig521end:12624-12643 MsG0080048897.01.T01:intron 40.0%
!! TTATCTCTTCTTGACTTGGG+CGG + contig521end:10616-10635 MsG0080048897.01.T01:CDS 40.0%
!!! CTTTTATTCAAGCTGGCTTG+TGG + contig521end:12124-12143 MsG0080048897.01.T01:intron 40.0%
!!! TTTGGTAGTGACACTGGTTT+TGG - contig521end:10566-10585 MsG0080048897.01.T01:intergenic 40.0%
AAAACATCCCTGTCCTAGTG+TGG - contig521end:12271-12290 MsG0080048897.01.T01:intergenic 45.0%
AAAACTCCGTGGATGCAGAA+TGG + contig521end:10344-10363 MsG0080048897.01.T01:CDS 45.0%
ACCTAGAACAAGTGTTTCGC+AGG - contig521end:11089-11108 MsG0080048897.01.T01:intergenic 45.0%
ACTATCTGCTCCCATTGGAT+TGG + contig521end:13441-13460 MsG0080048897.01.T01:exon 45.0%
ACTATTGAGGAGTTGTTCCG+TGG - contig521end:11797-11816 MsG0080048897.01.T01:intergenic 45.0%
ATGGGTCAAATCTGCATCGA+TGG - contig521end:12504-12523 MsG0080048897.01.T01:intergenic 45.0%
CAGCATGATGCCTTCATTGA+AGG + contig521end:11658-11677 MsG0080048897.01.T01:CDS 45.0%
GAAGAAGAACTTGTCAAGCC+CGG - contig521end:11414-11433 MsG0080048897.01.T01:intergenic 45.0%
GAGAAACCGTTGACAAATGG+CGG + contig521end:10182-10201 MsG0080048897.01.T01:CDS 45.0%
GAGATGGTTGCATGTATTGG+TGG + contig521end:11500-11519 MsG0080048897.01.T01:CDS 45.0%
GCAGAGAAACCGTTGACAAA+TGG + contig521end:10179-10198 MsG0080048897.01.T01:CDS 45.0%
GCTAAAGATGCATCATCGAC+GGG - contig521end:10646-10665 MsG0080048897.01.T01:intergenic 45.0%
TAACCTTCCTGTCTGTACTC+TGG + contig521end:12687-12706 MsG0080048897.01.T01:intron 45.0%
TCCTGCGAAACACTTGTTCT+AGG + contig521end:11085-11104 MsG0080048897.01.T01:intron 45.0%
TCTGCTCTTTGGCCTATTTC+AGG + contig521end:10436-10455 MsG0080048897.01.T01:CDS 45.0%
TTTCCTGTCCTTTCTGGACT+TGG + contig521end:11382-11401 MsG0080048897.01.T01:CDS 45.0%
TTTGAGAGGAGAAAACTCCG+TGG + contig521end:10333-10352 MsG0080048897.01.T01:CDS 45.0%
! CATTGATTGGCTCACAGATC+TGG + contig521end:11967-11986 MsG0080048897.01.T01:intron 45.0%
! CTTAAACTGATGGACGTCAG+AGG + contig521end:13000-13019 MsG0080048897.01.T01:CDS 45.0%
! GTCAAATCTGCATCGATGGT+TGG - contig521end:12500-12519 MsG0080048897.01.T01:intergenic 45.0%
! TGCATCTTTAGCTTCGTTGC+AGG + contig521end:10654-10673 MsG0080048897.01.T01:CDS 45.0%
!! GCTGGATTAGGGATTTTAGC+TGG + contig521end:11566-11585 MsG0080048897.01.T01:CDS 45.0%
!! GGATTAGCTCTGCTGGATTA+GGG + contig521end:11555-11574 MsG0080048897.01.T01:CDS 45.0%
!! GTGGAAGAGGTTGATTTGAG+AGG + contig521end:10319-10338 MsG0080048897.01.T01:CDS 45.0%
!! TGGATTAGCTCTGCTGGATT+AGG + contig521end:11554-11573 MsG0080048897.01.T01:CDS 45.0%
AAACTTCGAGGAGAGATGGG+TGG - contig521end:10288-10307 MsG0080048897.01.T01:intergenic 50.0%
AAAGACAGCCACACTAGGAC+AGG + contig521end:12260-12279 MsG0080048897.01.T01:intron 50.0%
AAGACAGCCACACTAGGACA+GGG + contig521end:12261-12280 MsG0080048897.01.T01:intron 50.0%
AGAACTTGTCAAGCCCGGTA+TGG - contig521end:11409-11428 MsG0080048897.01.T01:intergenic 50.0%
AGTCCAGAGTACAGACAGGA+AGG - contig521end:12693-12712 MsG0080048897.01.T01:intergenic 50.0%
AGTGAGATGTCGGCAAGTGA+AGG - contig521end:12883-12902 MsG0080048897.01.T01:intergenic 50.0%
CAAAGACTGTGCAAGCAGCT+AGG + contig521end:13160-13179 MsG0080048897.01.T01:CDS 50.0%
CTGTCTGTACTCTGGACTCA+GGG + contig521end:12695-12714 MsG0080048897.01.T01:intron 50.0%
GAACTTGTCAAGCCCGGTAT+GGG - contig521end:11408-11427 MsG0080048897.01.T01:intergenic 50.0%
GGCAAGAAGCTTGACACCTT+TGG - contig521end:10584-10603 MsG0080048897.01.T01:intergenic 50.0%
TGCCATCCATTCTGCATCCA+CGG - contig521end:10353-10372 MsG0080048897.01.T01:intergenic 50.0%
TTTCTGGACTTGGCCCATAC+CGG + contig521end:11392-11411 MsG0080048897.01.T01:CDS 50.0%
! GACTCAGGGTTTTTGCACCA+GGG + contig521end:12709-12728 MsG0080048897.01.T01:intron 50.0%
! GCACCAGGGAAGTTCTGATT+TGG + contig521end:12723-12742 MsG0080048897.01.T01:CDS 50.0%
! TGATGGACGTCAGAGGTTGT+TGG + contig521end:13007-13026 MsG0080048897.01.T01:CDS 50.0%
!! GACACCTTTGGTAGTGACAC+TGG - contig521end:10572-10591 MsG0080048897.01.T01:intergenic 50.0%
!! GGTACTTGGATTAGCTCTGC+TGG + contig521end:11548-11567 MsG0080048897.01.T01:CDS 50.0%
CCTGAGTCCAGAGTACAGAC+AGG - contig521end:12697-12716 MsG0080048897.01.T01:intergenic 55.0%
CCTGTCTGTACTCTGGACTC+AGG + contig521end:12694-12713 MsG0080048897.01.T01:intron 55.0%
CTCCGTGGATGCAGAATGGA+TGG + contig521end:10348-10367 MsG0080048897.01.T01:CDS 55.0%
GGTATGGGCCAAGTCCAGAA+AGG - contig521end:11393-11412 MsG0080048897.01.T01:intergenic 55.0%
TTCTGGACTTGGCCCATACC+GGG + contig521end:11393-11412 MsG0080048897.01.T01:CDS 55.0%
! GGACTCAGGGTTTTTGCACC+AGG + contig521end:12708-12727 MsG0080048897.01.T01:intron 55.0%
CTTCGAGGAGAGATGGGTGG+AGG - contig521end:10285-10304 MsG0080048897.01.T01:intergenic 60.0%
GGAGAGATGGGTGGAGGAGA+CGG - contig521end:10279-10298 MsG0080048897.01.T01:intergenic 60.0%
GGGCCAAGTCCAGAAAGGAC+AGG - contig521end:11388-11407 MsG0080048897.01.T01:intergenic 60.0%
GGAGACGGCGAGTGATGAGG+CGG - contig521end:10264-10283 MsG0080048897.01.T01:intergenic 65.0%
GGAGGAGACGGCGAGTGATG+AGG - contig521end:10267-10286 MsG0080048897.01.T01:intergenic 65.0%
!! AGAGCGTCGGCGAGAGGAGA+AGG - contig521end:10235-10254 MsG0080048897.01.T01:intergenic 65.0%
GACGGCGAGTGATGAGGCGG+CGG - contig521end:10261-10280 MsG0080048897.01.T01:intergenic 70.0%
! GAGGCGGCGGAGAAGAGCGT+CGG - contig521end:10248-10267 MsG0080048897.01.T01:intergenic 70.0%
!! CGGAGAAGAGCGTCGGCGAG+AGG - contig521end:10241-10260 MsG0080048897.01.T01:intergenic 70.0%
Chromosome Type Strat End Strand Name
contig521end gene 10006 13542 10006 ID=MsG0080048897.01;
contig521end mRNA 10006 13542 10006 ID=MsG0080048897.01.T01;Parent=MsG0080048897.01
contig521end exon 10006 10675 10006 ID=MsG0080048897.01.T01.exon;Parent=MsG0080048897.01.T01
contig521end CDS 10127 10675 10127 ID=MsG0080048897.01.T01.cds;Parent=MsG0080048897.01.T01
contig521end CDS 11107 11710 11107 ID=MsG0080048897.01.T01.cds;Parent=MsG0080048897.01.T01
contig521end exon 11107 11710 11107 ID=MsG0080048897.01.T01.exon;Parent=MsG0080048897.01.T01
contig521end CDS 12716 13219 12716 ID=MsG0080048897.01.T01.cds;Parent=MsG0080048897.01.T01
contig521end exon 12716 13219 12716 ID=MsG0080048897.01.T01.exon;Parent=MsG0080048897.01.T01
contig521end CDS 13305 13417 13305 ID=MsG0080048897.01.T01.cds;Parent=MsG0080048897.01.T01
contig521end exon 13305 13542 13305 ID=MsG0080048897.01.T01.exon;Parent=MsG0080048897.01.T01
Gene Sequence

>MsG0080048897.01.T01

ATGAAGAGCGAAATCTCATTGGTTGAACTCTGCATAGACGCAGCTTGTAAAAGCAGAGAAACCGTTGACAAATGGCGGTTACAAAGACGGTCTCTCGATTGCTTACCTTCTCCTCTCGCCGACGCTCTTCTCCGCCGCCTCATCACTCGCCGTCTCCTCCACCCATCTCTCCTCGAAGTTTTCAAACACAGTGTGGAAGAGGTTGATTTGAGAGGAGAAAACTCCGTGGATGCAGAATGGATGGCATATTTAGGAGCATTTCGCCATTTACGTTATTTGAACCTTGCTGAATGTCATCGAATCAATTCATCTGCTCTTTGGCCTATTTCAGGAATGAATAGCCTTAAAGACTTGGATCTATCTAGATGCTCCAAATTTAATGATGCTGGAATTAATCATATCCTTTCTGTTCCTAATTTAGAGAAGTTGCGCATTTCCAAAACCAGTGTCACTACCAAAGGTGTCAAGCTTCTTGCCTCTCTTAAACACTTATCTCTTCTTGACTTGGGCGGTTTGCCCGTCGATGATGCATCTTTAGCTTCGTTGCAGGTACTGAAAAATCTACAACACATTGAACTCTGGGACAGTAAAATATCAAACCAAGGTGCTGCTATTCTTAATACGTTCCCCAAGTTGACCCATCTAAATCTTGATTGGACTAGCGTCACAAAATTGCCTGATTTAACATCTCTTGAATGCCTCAACATGAGTAATTGCACCATTGATTCTATTCTCAAAGATGGTAAAGCTCCTTTGACAAAGCTTATTTTCTCTGGGTCTACATTCCTGAATGAAGCTGAAACCTTATTGTATGCAAATACATATTTCCTGTCCTTTCTGGACTTGGCCCATACCGGGCTTGACAAGTTCTTCTTCTTAAGTAAGCTGAAAGTAATTGAACATCTCAATCTCAGCTCATGTATGATGGATGATGATTCAGTTGAGATGGTTGCATGTATTGGTGGAAAATTGAAAAGTTTGAATCTGAGTGGTACTTGGATTAGCTCTGCTGGATTAGGGATTTTAGCTGGACATGTTCCCAATCTTGAAATTCTTTCACTATCTCAGACATCAGTCGATGATACTGCCCTCTTATATATCAGCATGATGCCTTCATTGAAGGATGTTGATCTGAGCAATACAAACATTAAAGGGTTTTTGCACCAGGGAAGTTCTGATTTGGATTCGCTCTCTCTAACCGCCCTACAAAATCTTAATCAATTACAAAGGTTGAATTTGGAGCACACTCAAGTCACCGATGAAGCTTTATTCCCTTTATCAAGCTTCCAAGAACTGAGATATTTATCTCTTAAAAGTCCTTCACTTGCCGACATCTCACTTTACCATCTATCATCAGTTCCTAAGTTGACAAAGTTTAGGATTTGTGATGCGGTTTTGACAAATTATGCTTTTGATATGTTTAAGACTCCTGAAACTCTTAAACTGATGGACGTCAGAGGTTGTTGGCTCTTGACAAAAGAAGCTATTGTATCATTCTGTAAAAATCACCCACAAATTGAAGTAAGGCATGAACTTGTTACTGTACTTCCTTTTGAAGAAAATGGACTTCATCATTCATCTCCATCTCGATTAACTTCAAAGACTGTGCAAGCAGCTAGGAAGAAAGAACAAACCTCCTTATCTCCAAATTTTGTAGATCAAAGGATGAAATATAGTAGAGATGAATTACTTGCATTGCAGTTTATGTCGTTGTCTCTTGCCTCCAGTAGTGAAATAGACAACTCAATACTTGAGAAGCAATTGGATTGA
Protein sequence

>MsG0080048897.01.T01

MKSEISLVELCIDAACKSRETVDKWRLQRRSLDCLPSPLADALLRRLITRRLLHPSLLEVFKHSVEEVDLRGENSVDAEWMAYLGAFRHLRYLNLAECHRINSSALWPISGMNSLKDLDLSRCSKFNDAGINHILSVPNLEKLRISKTSVTTKGVKLLASLKHLSLLDLGGLPVDDASLASLQVLKNLQHIELWDSKISNQGAAILNTFPKLTHLNLDWTSVTKLPDLTSLECLNMSNCTIDSILKDGKAPLTKLIFSGSTFLNEAETLLYANTYFLSFLDLAHTGLDKFFFLSKLKVIEHLNLSSCMMDDDSVEMVACIGGKLKSLNLSGTWISSAGLGILAGHVPNLEILSLSQTSVDDTALLYISMMPSLKDVDLSNTNIKGFLHQGSSDLDSLSLTALQNLNQLQRLNLEHTQVTDEALFPLSSFQELRYLSLKSPSLADISLYHLSSVPKLTKFRICDAVLTNYAFDMFKTPETLKLMDVRGCWLLTKEAIVSFCKNHPQIEVRHELVTVLPFEENGLHHSSPSRLTSKTVQAARKKEQTSLSPNFVDQRMKYSRDELLALQFMSLSLASSSEIDNSILEKQLD*