Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049034.01.T01 | XP_003626375.2 | 95.833 | 168 | 7 | 0 | 1 | 168 | 1 | 168 | 6.94E-117 | 340 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049034.01.T01 | G7L0S2 | 95.833 | 168 | 7 | 0 | 1 | 168 | 1 | 168 | 3.32e-117 | 340 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048773.01 | MsG0080049034.01 | 0.805822 | 1.108665e-49 | 5.923766e-47 |
| MsG0080049034.01 | MsG0180001610.01 | 0.800188 | 1.620356e-48 | 7.497001e-46 |
| MsG0080049034.01 | MsG0280006996.01 | 0.806847 | 6.743315e-50 | 3.698793e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049034.01.T01 | MTR_7g114390 | 95.833 | 168 | 7 | 0 | 1 | 168 | 1 | 168 | 8.41e-121 | 340 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049034.01.T01 | AT3G04950 | 58.389 | 149 | 58 | 2 | 23 | 168 | 41 | 188 | 3.14e-59 | 184 |
Find 32 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCAACTATATACTCTTTAA+AGG | 0.178788 | contig623end:+11203 | None:intergenic |
| CGGTTCACCTTCAGAGTTCA+TGG | 0.336324 | contig623end:-11412 | MsG0080049034.01.T01:CDS |
| ATGCACTTGCAAAGTTTACT+TGG | 0.358285 | contig623end:-10954 | MsG0080049034.01.T01:CDS |
| TGCATCTGGTGGTGCTCCTT+CGG | 0.382519 | contig623end:+11352 | None:intergenic |
| GAGTTAAAGGTATCGAATAA+TGG | 0.404497 | contig623end:-11519 | MsG0080049034.01.T01:exon |
| GAAAATGAACTTACGGAGTA+AGG | 0.422469 | contig623end:+11319 | None:intergenic |
| AAGACTGGGAAGTGGAAAAC+TGG | 0.423192 | contig623end:+11557 | None:intergenic |
| GCCTTTAAAGAGTATATAGT+TGG | 0.433265 | contig623end:-11204 | MsG0080049034.01.T01:CDS |
| AATCGAATGCGGCGAGATAT+CGG | 0.442505 | contig623end:+11127 | None:intergenic |
| TCTTGCTTTCTTCATTTCAT+CGG | 0.458556 | contig623end:+11236 | None:intergenic |
| ATGAAGAAAGCAAGAAAAGT+TGG | 0.472505 | contig623end:-11228 | MsG0080049034.01.T01:CDS |
| GATGAAGGTATTATTAAGTA+AGG | 0.489931 | contig623end:+10848 | None:intergenic |
| AATGCCTTCGTATTTCTCAA+TGG | 0.521342 | contig623end:+11152 | None:intergenic |
| TGCACTTGCAAAGTTTACTT+GGG | 0.524528 | contig623end:-10953 | MsG0080049034.01.T01:CDS |
| TAAGTGCACAATTGCTGAAG+TGG | 0.546585 | contig623end:-10980 | MsG0080049034.01.T01:CDS |
| CCACCGTTCAATTTCCACTA+CGG | 0.551541 | contig623end:-11432 | MsG0080049034.01.T01:CDS |
| ATTCAAAAGTTAAATCAAGA+AGG | 0.556894 | contig623end:-10910 | MsG0080049034.01.T01:CDS |
| TCACCTTCAGAGTTCATGGA+TGG | 0.571753 | contig623end:-11408 | MsG0080049034.01.T01:CDS |
| GAAGGTATTATTAAGTAAGG+AGG | 0.579013 | contig623end:+10851 | None:intergenic |
| TAGTGGAAATTGAACGGTGG+TGG | 0.584928 | contig623end:+11435 | None:intergenic |
| TGTCCATCCATGAACTCTGA+AGG | 0.596448 | contig623end:+11405 | None:intergenic |
| CGCATTCGATTACACAGGAG+AGG | 0.602676 | contig623end:-11115 | MsG0080049034.01.T01:intron |
| AAGGTGAATTGAGATGCATC+TGG | 0.611379 | contig623end:+11338 | None:intergenic |
| ACTCTGAAGGTGAACCGTAG+TGG | 0.617132 | contig623end:+11418 | None:intergenic |
| CTCTCCTGTGTAATCGAATG+CGG | 0.644799 | contig623end:+11116 | None:intergenic |
| GAACCGTAGTGGAAATTGAA+CGG | 0.645642 | contig623end:+11429 | None:intergenic |
| TCAGAGTTCATGGATGGACA+AGG | 0.658450 | contig623end:-11402 | MsG0080049034.01.T01:CDS |
| TCTGTTTGCGATGCGAAGTG+CGG | 0.662046 | contig623end:+11496 | None:intergenic |
| ACTGCCATTGAGAAATACGA+AGG | 0.691670 | contig623end:-11156 | MsG0080049034.01.T01:CDS |
| CTCGCCGCATTCGATTACAC+AGG | 0.703771 | contig623end:-11120 | MsG0080049034.01.T01:CDS |
| GTGAATTGAGATGCATCTGG+TGG | 0.748795 | contig623end:+11341 | None:intergenic |
| CCGTAGTGGAAATTGAACGG+TGG | 0.810192 | contig623end:+11432 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTCAAAAGTTAAATCAAGA+AGG | - | contig623end:11523-11542 | MsG0080049034.01.T01:five_prime_UTR | 20.0% |
| !!! | ATTACTTCAGACATATTTTT+AGG | + | contig623end:11559-11578 | None:intergenic | 20.0% |
| !!! | TTGTTGATTTTGTTTTGTTA+TGG | - | contig623end:11385-11404 | MsG0080049034.01.T01:CDS | 20.0% |
| ! | GAAATGAGAAAATGAACTTA+CGG | + | contig623end:11124-11143 | None:intergenic | 25.0% |
| ! | TCCAACTATATACTCTTTAA+AGG | + | contig623end:11233-11252 | None:intergenic | 25.0% |
| !! | AAAAACAGGATTTGAGTTAA+AGG | - | contig623end:10901-10920 | MsG0080049034.01.T01:CDS | 25.0% |
| AACGAATCAAAAACATGAAG+CGG | - | contig623end:11420-11439 | MsG0080049034.01.T01:CDS | 30.0% | |
| ATGAAGAAAGCAAGAAAAGT+TGG | - | contig623end:11205-11224 | MsG0080049034.01.T01:CDS | 30.0% | |
| TCTTGCTTTCTTCATTTCAT+CGG | + | contig623end:11200-11219 | None:intergenic | 30.0% | |
| ! | GCCTTTAAAGAGTATATAGT+TGG | - | contig623end:11229-11248 | MsG0080049034.01.T01:CDS | 30.0% |
| !! | GAGTTAAAGGTATCGAATAA+TGG | - | contig623end:10914-10933 | MsG0080049034.01.T01:CDS | 30.0% |
| !!! | AAATCCTGTTTTTGTAAGAC+TGG | + | contig623end:10894-10913 | None:intergenic | 30.0% |
| !!! | AATCCTGTTTTTGTAAGACT+GGG | + | contig623end:10893-10912 | None:intergenic | 30.0% |
| !!! | TCAGACATATTTTTAGGCAT+GGG | + | contig623end:11553-11572 | None:intergenic | 30.0% |
| !!! | TTCAGACATATTTTTAGGCA+TGG | + | contig623end:11554-11573 | None:intergenic | 30.0% |
| AATGCCTTCGTATTTCTCAA+TGG | + | contig623end:11284-11303 | None:intergenic | 35.0% | |
| ATGCACTTGCAAAGTTTACT+TGG | - | contig623end:11479-11498 | MsG0080049034.01.T01:CDS | 35.0% | |
| GAAAATGAACTTACGGAGTA+AGG | + | contig623end:11117-11136 | None:intergenic | 35.0% | |
| TGCACTTGCAAAGTTTACTT+GGG | - | contig623end:11480-11499 | MsG0080049034.01.T01:CDS | 35.0% | |
| AAGGTCAAAAACGTCTTCAC+AGG | - | contig623end:11050-11069 | MsG0080049034.01.T01:intron | 40.0% | |
| AAGGTGAATTGAGATGCATC+TGG | + | contig623end:11098-11117 | None:intergenic | 40.0% | |
| ACTGCCATTGAGAAATACGA+AGG | - | contig623end:11277-11296 | MsG0080049034.01.T01:intron | 40.0% | |
| CTTCCCAGTCTTACAAAAAC+AGG | - | contig623end:10887-10906 | MsG0080049034.01.T01:CDS | 40.0% | |
| TAAGTGCACAATTGCTGAAG+TGG | - | contig623end:11453-11472 | MsG0080049034.01.T01:CDS | 40.0% | |
| ! | GAACCGTAGTGGAAATTGAA+CGG | + | contig623end:11007-11026 | None:intergenic | 40.0% |
| ! | GCAAACTCTGAGGACAAAAT+AGG | + | contig623end:11178-11197 | None:intergenic | 40.0% |
| ! | GTTTTTGTAAGACTGGGAAG+TGG | + | contig623end:10887-10906 | None:intergenic | 40.0% |
| AAGACTGGGAAGTGGAAAAC+TGG | + | contig623end:10879-10898 | None:intergenic | 45.0% | |
| AATCGAATGCGGCGAGATAT+CGG | + | contig623end:11309-11328 | None:intergenic | 45.0% | |
| CATTTCATCGGCAAACTCTG+AGG | + | contig623end:11188-11207 | None:intergenic | 45.0% | |
| CCACCGTTCAATTTCCACTA+CGG | - | contig623end:11001-11020 | MsG0080049034.01.T01:CDS | 45.0% | |
| CTCTCCTGTGTAATCGAATG+CGG | + | contig623end:11320-11339 | None:intergenic | 45.0% | |
| GTGAATTGAGATGCATCTGG+TGG | + | contig623end:11095-11114 | None:intergenic | 45.0% | |
| TAGTGGAAATTGAACGGTGG+TGG | + | contig623end:11001-11020 | None:intergenic | 45.0% | |
| TCACCTTCAGAGTTCATGGA+TGG | - | contig623end:11025-11044 | MsG0080049034.01.T01:intron | 45.0% | |
| TCAGAGTTCATGGATGGACA+AGG | - | contig623end:11031-11050 | MsG0080049034.01.T01:intron | 45.0% | |
| TGTCCATCCATGAACTCTGA+AGG | + | contig623end:11031-11050 | None:intergenic | 45.0% | |
| TTCACAGGCCAAAAATCCGA+AGG | - | contig623end:11065-11084 | MsG0080049034.01.T01:intron | 45.0% | |
| CGCATTCGATTACACAGGAG+AGG | - | contig623end:11318-11337 | MsG0080049034.01.T01:intron | 50.0% | |
| CGGTTCACCTTCAGAGTTCA+TGG | - | contig623end:11021-11040 | MsG0080049034.01.T01:CDS | 50.0% | |
| TCTGTTTGCGATGCGAAGTG+CGG | + | contig623end:10940-10959 | None:intergenic | 50.0% | |
| ! | ACTCTGAAGGTGAACCGTAG+TGG | + | contig623end:11018-11037 | None:intergenic | 50.0% |
| ! | CCGTAGTGGAAATTGAACGG+TGG | + | contig623end:11004-11023 | None:intergenic | 50.0% |
| ! | GTGGTGCTCCTTCGGATTTT+TGG | + | contig623end:11076-11095 | None:intergenic | 50.0% |
| CTCGCCGCATTCGATTACAC+AGG | - | contig623end:11313-11332 | MsG0080049034.01.T01:intron | 55.0% | |
| ! | TGCATCTGGTGGTGCTCCTT+CGG | + | contig623end:11084-11103 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig623end | gene | 10861 | 11594 | 10861 | ID=MsG0080049034.01;Name=MsG0080049034.01 |
| contig623end | mRNA | 10861 | 11594 | 10861 | ID=MsG0080049034.01.T01;Parent=MsG0080049034.01;Name=MsG0080049034.01.T01;_AED=0.39;_eAED=0.40;_QI=72|1|0.66|1|1|1|3|0|173 |
| contig623end | exon | 11333 | 11594 | 11333 | ID=MsG0080049034.01.T01:exon:4636;Parent=MsG0080049034.01.T01 |
| contig623end | exon | 11116 | 11264 | 11116 | ID=MsG0080049034.01.T01:exon:4637;Parent=MsG0080049034.01.T01 |
| contig623end | exon | 10861 | 11043 | 10861 | ID=MsG0080049034.01.T01:exon:4638;Parent=MsG0080049034.01.T01 |
| contig623end | five_prime_UTR | 11523 | 11594 | 11523 | ID=MsG0080049034.01.T01:five_prime_utr;Parent=MsG0080049034.01.T01 |
| contig623end | CDS | 11333 | 11522 | 11333 | ID=MsG0080049034.01.T01:cds;Parent=MsG0080049034.01.T01 |
| contig623end | CDS | 11116 | 11264 | 11116 | ID=MsG0080049034.01.T01:cds;Parent=MsG0080049034.01.T01 |
| contig623end | CDS | 10861 | 11043 | 10861 | ID=MsG0080049034.01.T01:cds;Parent=MsG0080049034.01.T01 |
| Gene Sequence |
| Protein sequence |
