Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049159.01.T01 | YP_009237637.1 | 100 | 125 | 0 | 0 | 1 | 125 | 1 | 125 | 1.89E-84 | 252 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049159.01.T01 | P27070 | 94.4 | 125 | 7 | 0 | 1 | 125 | 1 | 125 | 1.35E-80 | 235 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049159.01.T01 | A0A126TGT6 | 100.000 | 125 | 0 | 0 | 1 | 125 | 1 | 125 | 9.02e-85 | 252 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0480018565.01 | MsG0080049159.01 | PPI |
| MsG0080049159.01 | MsG0680030366.01 | PPI |
| MsG0680030366.01 | MsG0080049159.01 | PPI |
| MsG0080049159.01 | MsG0780041076.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049159.01.T01 | MTR_0082s0040 | 100.000 | 125 | 0 | 0 | 1 | 125 | 238 | 362 | 4.80e-87 | 257 |
| MsG0080049159.01.T01 | MTR_0625s0020 | 67.470 | 83 | 27 | 0 | 41 | 123 | 15 | 97 | 2.78e-36 | 120 |
| MsG0080049159.01.T01 | MTR_7g055840 | 66.667 | 81 | 27 | 0 | 43 | 123 | 13 | 93 | 1.28e-34 | 115 |
| MsG0080049159.01.T01 | MTR_7g055910 | 65.060 | 83 | 29 | 0 | 41 | 123 | 113 | 195 | 6.21e-33 | 114 |
| MsG0080049159.01.T01 | MTR_3g035660 | 45.600 | 125 | 65 | 1 | 1 | 125 | 53 | 174 | 2.60e-29 | 111 |
| MsG0080049159.01.T01 | MTR_0002s0280 | 64.000 | 75 | 27 | 0 | 42 | 116 | 83 | 157 | 1.94e-28 | 102 |
| MsG0080049159.01.T01 | MTR_1g084830 | 94.872 | 39 | 2 | 0 | 8 | 46 | 112 | 150 | 3.79e-19 | 78.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080049159.01.T01 | ATMG00980 | 91.200 | 125 | 11 | 0 | 1 | 125 | 1 | 125 | 1.97e-78 | 227 |
| MsG0080049159.01.T01 | AT2G07675 | 91.200 | 125 | 11 | 0 | 1 | 125 | 1 | 125 | 1.97e-78 | 227 |
| MsG0080049159.01.T01 | ATCG01230 | 66.265 | 83 | 28 | 0 | 41 | 123 | 3 | 85 | 8.52e-36 | 118 |
| MsG0080049159.01.T01 | ATCG00905 | 66.265 | 83 | 28 | 0 | 41 | 123 | 3 | 85 | 8.52e-36 | 118 |
Find 32 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCGGGCGTACTCCTTGCTTC+TGG | 0.225224 | contig97end:+19326 | MsG0080049159.01.T01:intergenic |
| CAAGGATTTGTTGGGAATTC+CGG | 0.244708 | contig97end:-19107 | MsG0080049159.01.T01:CDS |
| ATCTTACGTTGAGCTGAATT+TGG | 0.247376 | contig97end:+19277 | MsG0080049159.01.T01:intergenic |
| TGATATATTTGCTCACATTC+CGG | 0.302524 | contig97end:-19227 | MsG0080049159.01.T01:CDS |
| ATCCTCCATTCATATCGATT+TGG | 0.306818 | contig97end:+19045 | MsG0080049159.01.T01:intergenic |
| TCCTCCATTCATATCGATTT+GGG | 0.309921 | contig97end:+19046 | MsG0080049159.01.T01:intergenic |
| CGGGCGTACTCCTTGCTTCT+GGG | 0.326117 | contig97end:+19327 | MsG0080049159.01.T01:intergenic |
| GATATATTTGCTCACATTCC+GGG | 0.354563 | contig97end:-19226 | MsG0080049159.01.T01:CDS |
| CGAGGAGTCAAGGATTTGTT+GGG | 0.416175 | contig97end:-19115 | MsG0080049159.01.T01:CDS |
| TCGAGGAGTCAAGGATTTGT+TGG | 0.418048 | contig97end:-19116 | MsG0080049159.01.T01:CDS |
| AGAAGAGGCAGATCAAAATA+TGG | 0.430997 | contig97end:-19079 | MsG0080049159.01.T01:CDS |
| CGAATCAATTGATTTAATGT+AGG | 0.458996 | contig97end:+19406 | MsG0080049159.01.T01:intergenic |
| CACGGACCGTACTCGAGCTT+CGG | 0.459749 | contig97end:-19359 | MsG0080049159.01.T01:CDS |
| TCGATTGCTCAATCGTACTT+TGG | 0.467988 | contig97end:+19252 | MsG0080049159.01.T01:intergenic |
| GATCTGCCTCTTCTTCGATC+CGG | 0.495631 | contig97end:+19088 | MsG0080049159.01.T01:intergenic |
| CTTGACTCCTCGAATACAAT+GGG | 0.496906 | contig97end:+19126 | MsG0080049159.01.T01:intergenic |
| CATTCTACGGTCTTAATAAG+AGG | 0.509133 | contig97end:-19181 | MsG0080049159.01.T01:CDS |
| GGTAGAGTTAAAGATTTGCC+AGG | 0.536394 | contig97end:-19157 | MsG0080049159.01.T01:CDS |
| TTTGCTCACATTCCGGGCGA+AGG | 0.538531 | contig97end:-19220 | MsG0080049159.01.T01:CDS |
| ATTGATCCGAAGCTCGAGTA+CGG | 0.542451 | contig97end:+19353 | MsG0080049159.01.T01:intergenic |
| GTGAAATCCCATTGTATTCG+AGG | 0.549831 | contig97end:-19133 | MsG0080049159.01.T01:CDS |
| CCTTGACTCCTCGAATACAA+TGG | 0.552314 | contig97end:+19125 | MsG0080049159.01.T01:intergenic |
| CCATTGTATTCGAGGAGTCA+AGG | 0.553703 | contig97end:-19125 | MsG0080049159.01.T01:CDS |
| GGGCGAAGGTCATAATTCGC+AGG | 0.554524 | contig97end:-19206 | MsG0080049159.01.T01:CDS |
| TGCGAATTATGACCTTCGCC+CGG | 0.559329 | contig97end:+19208 | MsG0080049159.01.T01:intergenic |
| TAATTCGCAGGAACATTCTA+CGG | 0.570921 | contig97end:-19194 | MsG0080049159.01.T01:CDS |
| TTAAATCAATTGATTCGTCA+TGG | 0.572229 | contig97end:-19400 | MsG0080049159.01.T01:CDS |
| ATACAATGGGATTTCACACC+TGG | 0.575815 | contig97end:+19139 | MsG0080049159.01.T01:intergenic |
| GGGCGTACTCCTTGCTTCTG+GGG | 0.603634 | contig97end:+19328 | MsG0080049159.01.T01:intergenic |
| GATCAATGTCCCCAGAAGCA+AGG | 0.611270 | contig97end:-19337 | MsG0080049159.01.T01:CDS |
| GGAATTCCGGATCGAAGAAG+AGG | 0.614106 | contig97end:-19094 | MsG0080049159.01.T01:CDS |
| TCTACGGTCTTAATAAGAGG+AGG | 0.670870 | contig97end:-19178 | MsG0080049159.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CGAATCAATTGATTTAATGT+AGG | + | contig97end:19060-19079 | MsG0080049159.01.T01:intergenic | 25.0% |
| !!! | TTAAATCAATTGATTCGTCA+TGG | - | contig97end:19063-19082 | MsG0080049159.01.T01:CDS | 25.0% |
| TGATATATTTGCTCACATTC+CGG | - | contig97end:19236-19255 | MsG0080049159.01.T01:CDS | 30.0% | |
| !!! | TGAGCTGAATTTGGTTTTTT+CGG | + | contig97end:19180-19199 | MsG0080049159.01.T01:intergenic | 30.0% |
| AGAAGAGGCAGATCAAAATA+TGG | - | contig97end:19384-19403 | MsG0080049159.01.T01:CDS | 35.0% | |
| ATCTTACGTTGAGCTGAATT+TGG | + | contig97end:19189-19208 | MsG0080049159.01.T01:intergenic | 35.0% | |
| CATTCTACGGTCTTAATAAG+AGG | - | contig97end:19282-19301 | MsG0080049159.01.T01:CDS | 35.0% | |
| GATATATTTGCTCACATTCC+GGG | - | contig97end:19237-19256 | MsG0080049159.01.T01:CDS | 35.0% | |
| GTCATGGTAGAGAAGAAAAA+CGG | - | contig97end:19079-19098 | MsG0080049159.01.T01:CDS | 35.0% | |
| TAATTCGCAGGAACATTCTA+CGG | - | contig97end:19269-19288 | MsG0080049159.01.T01:CDS | 35.0% | |
| ATACAATGGGATTTCACACC+TGG | + | contig97end:19327-19346 | MsG0080049159.01.T01:intergenic | 40.0% | |
| CTTGACTCCTCGAATACAAT+GGG | + | contig97end:19340-19359 | MsG0080049159.01.T01:intergenic | 40.0% | |
| GGTAGAGTTAAAGATTTGCC+AGG | - | contig97end:19306-19325 | MsG0080049159.01.T01:CDS | 40.0% | |
| GGTGTTCTCTTAAAAACACG+CGG | + | contig97end:19159-19178 | MsG0080049159.01.T01:intergenic | 40.0% | |
| GTGAAATCCCATTGTATTCG+AGG | - | contig97end:19330-19349 | MsG0080049159.01.T01:CDS | 40.0% | |
| GTGTTCTCTTAAAAACACGC+GGG | + | contig97end:19158-19177 | MsG0080049159.01.T01:intergenic | 40.0% | |
| TCGATTGCTCAATCGTACTT+TGG | + | contig97end:19214-19233 | MsG0080049159.01.T01:intergenic | 40.0% | |
| TCTACGGTCTTAATAAGAGG+AGG | - | contig97end:19285-19304 | MsG0080049159.01.T01:CDS | 40.0% | |
| !! | CAAGGATTTGTTGGGAATTC+CGG | - | contig97end:19356-19375 | MsG0080049159.01.T01:CDS | 40.0% |
| ATTGATCCGAAGCTCGAGTA+CGG | + | contig97end:19113-19132 | MsG0080049159.01.T01:intergenic | 45.0% | |
| CCATTGTATTCGAGGAGTCA+AGG | - | contig97end:19338-19357 | MsG0080049159.01.T01:CDS | 45.0% | |
| CCTTGACTCCTCGAATACAA+TGG | + | contig97end:19341-19360 | MsG0080049159.01.T01:intergenic | 45.0% | |
| TAGAGAAGAAAAACGGCGCA+CGG | - | contig97end:19086-19105 | MsG0080049159.01.T01:CDS | 45.0% | |
| TCGAGGAGTCAAGGATTTGT+TGG | - | contig97end:19347-19366 | MsG0080049159.01.T01:CDS | 45.0% | |
| ! | CGAGGAGTCAAGGATTTGTT+GGG | - | contig97end:19348-19367 | MsG0080049159.01.T01:CDS | 45.0% |
| GATCAATGTCCCCAGAAGCA+AGG | - | contig97end:19126-19145 | MsG0080049159.01.T01:CDS | 50.0% | |
| GATCTGCCTCTTCTTCGATC+CGG | + | contig97end:19378-19397 | MsG0080049159.01.T01:intergenic | 50.0% | |
| GGAATTCCGGATCGAAGAAG+AGG | - | contig97end:19369-19388 | MsG0080049159.01.T01:CDS | 50.0% | |
| TGCGAATTATGACCTTCGCC+CGG | + | contig97end:19258-19277 | MsG0080049159.01.T01:intergenic | 50.0% | |
| GGGCGAAGGTCATAATTCGC+AGG | - | contig97end:19257-19276 | MsG0080049159.01.T01:CDS | 55.0% | |
| TTTGCTCACATTCCGGGCGA+AGG | - | contig97end:19243-19262 | MsG0080049159.01.T01:CDS | 55.0% | |
| CACGGACCGTACTCGAGCTT+CGG | - | contig97end:19104-19123 | MsG0080049159.01.T01:CDS | 60.0% | |
| CGGGCGTACTCCTTGCTTCT+GGG | + | contig97end:19139-19158 | MsG0080049159.01.T01:intergenic | 60.0% | |
| GGGCGTACTCCTTGCTTCTG+GGG | + | contig97end:19138-19157 | MsG0080049159.01.T01:intergenic | 60.0% | |
| GCGGGCGTACTCCTTGCTTC+TGG | + | contig97end:19140-19159 | MsG0080049159.01.T01:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig97end | gene | 19054 | 19431 | 19054 | ID=MsG0080049159.01;Name=MsG0080049159.01 |
| contig97end | mRNA | 19054 | 19431 | 19054 | ID=MsG0080049159.01.T01;Parent=MsG0080049159.01;Name=MsG0080049159.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|125 |
| contig97end | exon | 19054 | 19431 | 19054 | ID=MsG0080049159.01.T01:exon:4822;Parent=MsG0080049159.01.T01 |
| contig97end | CDS | 19054 | 19431 | 19054 | ID=MsG0080049159.01.T01:cds;Parent=MsG0080049159.01.T01 |
| Gene Sequence |
| Protein sequence |
