AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680030366.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030366.01.T02 MTR_4g014360 94.340 106 3 1 1 106 1 103 5.51e-68 199
MsG0680030366.01.T02 MTR_6g005030 92.453 106 5 1 1 106 52 154 4.70e-65 193
MsG0680030366.01.T03 MTR_4g014360 87.302 63 8 0 1 63 1 63 1.40e-35 115
MsG0680030366.01.T03 MTR_6g005030 83.333 66 11 0 1 66 52 117 4.63e-35 116
MsG0680030366.01.T01 MTR_4g014360 97.087 103 3 0 1 103 1 103 3.52e-70 204
MsG0680030366.01.T01 MTR_6g005030 95.146 103 5 0 1 103 52 154 3.72e-67 199
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030366.01.T02 AT3G18880 65.138 109 31 3 1 106 1 105 1.87e-43 137
MsG0680030366.01.T02 AT3G18880 65.138 109 31 3 1 106 1 105 1.87e-43 137
MsG0680030366.01.T02 AT1G49400 76.190 84 17 1 1 84 1 81 8.83e-43 136
MsG0680030366.01.T03 AT1G49400 69.841 63 19 0 1 63 1 63 8.35e-28 96.7
MsG0680030366.01.T03 AT3G18880 68.254 63 20 0 1 63 1 63 1.45e-26 93.2
MsG0680030366.01.T03 AT3G18880 68.254 63 20 0 1 63 1 63 1.45e-26 93.2
MsG0680030366.01.T01 AT3G18880 66.981 106 31 2 1 103 1 105 1.22e-45 142
MsG0680030366.01.T01 AT3G18880 66.981 106 31 2 1 103 1 105 1.22e-45 142
MsG0680030366.01.T01 AT1G49400 79.012 81 17 0 1 81 1 81 6.89e-45 141
MsG0680030366.01.T01 AT1G79850 38.462 78 48 0 1 78 50 127 9.99e-12 57.8

Find 16 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAACGCACTTCCAAGTTTA+TGG 0.116836 6:+1125995 MsG0680030366.01.T01:CDS
AATACATATTCATCTGAATT+AGG 0.136683 6:+1125843 MsG0680030366.01.T01:five_prime_UTR
ATACATATTCATCTGAATTA+GGG 0.300965 6:+1125844 MsG0680030366.01.T01:five_prime_UTR
TGGAAGTGCGTTTAATATAT+CGG 0.349128 6:-1125986 None:intergenic
CATCTGAATTAGGGTTTGTT+GGG 0.369331 6:+1125853 MsG0680030366.01.T01:five_prime_UTR
TCATCTGAATTAGGGTTTGT+TGG 0.386146 6:+1125852 MsG0680030366.01.T01:five_prime_UTR
GAATTAGGGTTTGTTGGGAT+TGG 0.391008 6:+1125858 MsG0680030366.01.T01:five_prime_UTR
TAACATCACCGGTAAAAGAT+AGG 0.434833 6:-1126077 None:intergenic
TTCGAATAAGATGCAGAAAT+CGG 0.439794 6:+1125920 MsG0680030366.01.T01:CDS
GAGAAGATGAAGCAAGTAGT+AGG 0.480871 6:+1125888 MsG0680030366.01.T01:exon
GATGAAGCAAGTAGTAGGAA+TGG 0.481818 6:+1125893 MsG0680030366.01.T01:exon
TTCATCACGAGCCATAAACT+TGG 0.510999 6:-1126006 None:intergenic
TAAGATGCAGAAATCGGTTG+TGG 0.553827 6:+1125926 MsG0680030366.01.T01:CDS
GAAGCAAGTAGTAGGAATGG+TGG 0.579237 6:+1125896 MsG0680030366.01.T01:CDS
GAAAACAATCTTTGCAACAT+CGG 0.608322 6:+1126026 MsG0680030366.01.T01:CDS
AATTATTCACAATACTAACT+CGG 0.640432 6:-1126052 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTTTTTTGTGG+TGG - Chr6:1126287-1126306 None:intergenic 15.0%
!! AATACATATTCATCTGAATT+AGG + Chr6:1125843-1125862 MsG0680030366.01.T01:five_prime_UTR 20.0%
!! AATTATTCACAATACTAACT+CGG - Chr6:1126055-1126074 None:intergenic 20.0%
!! AGGTATTATAAAGTTAATCT+TGG + Chr6:1126605-1126624 MsG0680030366.01.T01:intron 20.0%
!! ATACATATTCATCTGAATTA+GGG + Chr6:1125844-1125863 MsG0680030366.01.T01:five_prime_UTR 20.0%
!!! ATAATTTACCTATCTTTTAC+CGG + Chr6:1126069-1126088 MsG0680030366.01.T01:intron 20.0%
! CTATAATTCATTAGCACAAA+TGG - Chr6:1127006-1127025 None:intergenic 25.0%
AGACAACAATTTGTGCTAAA+CGG - Chr6:1126161-1126180 None:intergenic 30.0%
CAAATTTATGACGTAGATGT+AGG - Chr6:1126581-1126600 None:intergenic 30.0%
GAAAACAATCTTTGCAACAT+CGG + Chr6:1126026-1126045 MsG0680030366.01.T01:CDS 30.0%
GACAATGCATAATATATGCA+AGG + Chr6:1126242-1126261 MsG0680030366.01.T01:intron 30.0%
GCTACAAATGAGTATCATAT+TGG - Chr6:1126765-1126784 None:intergenic 30.0%
GTGCTAATGAATTATAGTGT+AGG + Chr6:1127009-1127028 MsG0680030366.01.T01:three_prime_UTR 30.0%
TGGAATTGGAAATGAAGTTT+AGG + Chr6:1126669-1126688 MsG0680030366.01.T01:intron 30.0%
TTCGAATAAGATGCAGAAAT+CGG + Chr6:1125920-1125939 MsG0680030366.01.T01:CDS 30.0%
! ATAAGTGTCTTGTGCAATTT+TGG + Chr6:1126357-1126376 MsG0680030366.01.T01:intron 30.0%
! TAATGGAATGGTAATTTTCG+TGG + Chr6:1126844-1126863 MsG0680030366.01.T01:intron 30.0%
! TGGAAGTGCGTTTAATATAT+CGG - Chr6:1125989-1126008 None:intergenic 30.0%
! TTGTAGCAACTTCTAGTTAT+TGG + Chr6:1126778-1126797 MsG0680030366.01.T01:intron 30.0%
!! TATATCGGTTGAACATTTTG+TGG - Chr6:1125974-1125993 None:intergenic 30.0%
!!! TTTGAACAAACTGCTGTTTT+TGG + Chr6:1126326-1126345 MsG0680030366.01.T01:intron 30.0%
!!! TTTTGGATTTGCTGTGTTAT+TGG + Chr6:1126374-1126393 MsG0680030366.01.T01:intron 30.0%
!!! TTTTTTTTTTTTGTGGTGGC+CGG - Chr6:1126283-1126302 None:intergenic 30.0%
CATAATATATGCAAGGTCTG+GGG + Chr6:1126249-1126268 MsG0680030366.01.T01:intron 35.0%
GCATAATATATGCAAGGTCT+GGG + Chr6:1126248-1126267 MsG0680030366.01.T01:intron 35.0%
TAAACGCACTTCCAAGTTTA+TGG + Chr6:1125995-1126014 MsG0680030366.01.T01:CDS 35.0%
TAACATCACCGGTAAAAGAT+AGG - Chr6:1126080-1126099 None:intergenic 35.0%
TATATGCACTAGACTACAGT+CGG - Chr6:1126717-1126736 None:intergenic 35.0%
TCATCTGAATTAGGGTTTGT+TGG + Chr6:1125852-1125871 MsG0680030366.01.T01:five_prime_UTR 35.0%
TCCATTATATTCAGCAGCAT+GGG - Chr6:1126831-1126850 None:intergenic 35.0%
TGCATAATATATGCAAGGTC+TGG + Chr6:1126247-1126266 MsG0680030366.01.T01:intron 35.0%
TTCCATTATATTCAGCAGCA+TGG - Chr6:1126832-1126851 None:intergenic 35.0%
! CATCTGAATTAGGGTTTGTT+GGG + Chr6:1125853-1125872 MsG0680030366.01.T01:five_prime_UTR 35.0%
! CTACGTCATAAATTTGCTCA+AGG + Chr6:1126585-1126604 MsG0680030366.01.T01:intron 35.0%
! TGCTGCTGAATATAATGGAA+TGG + Chr6:1126832-1126851 MsG0680030366.01.T01:intron 35.0%
! TTTAGGTTTAGTTGAGTGAG+AGG + Chr6:1126686-1126705 MsG0680030366.01.T01:intron 35.0%
! TTTTAATGTGGCATGTTCCA+AGG + Chr6:1126876-1126895 MsG0680030366.01.T01:three_prime_UTR 35.0%
!!! TTTTTTTTTTTGTGGTGGCC+GGG - Chr6:1126282-1126301 None:intergenic 35.0%
AGGTTCGATTGGATTCTTCT+AGG + Chr6:1126444-1126463 MsG0680030366.01.T01:intron 40.0%
ATATGTTTCTCCCTCTGCAA+CGG + Chr6:1126523-1126542 MsG0680030366.01.T01:CDS 40.0%
CTGCAACTTCAGTTGACATA+AGG + Chr6:1126938-1126957 MsG0680030366.01.T01:three_prime_UTR 40.0%
GAGAAGATGAAGCAAGTAGT+AGG + Chr6:1125888-1125907 MsG0680030366.01.T01:exon 40.0%
GATGAAGCAAGTAGTAGGAA+TGG + Chr6:1125893-1125912 MsG0680030366.01.T01:exon 40.0%
TAAGATGCAGAAATCGGTTG+TGG + Chr6:1125926-1125945 MsG0680030366.01.T01:CDS 40.0%
TCCCATGCTGCTGAATATAA+TGG + Chr6:1126827-1126846 MsG0680030366.01.T01:intron 40.0%
TCTGAGCAAGCGTAAACATT+GGG + Chr6:1126469-1126488 MsG0680030366.01.T01:CDS 40.0%
TTACCGGTGATGTTAAACAG+CGG + Chr6:1126085-1126104 MsG0680030366.01.T01:intron 40.0%
TTCATCACGAGCCATAAACT+TGG - Chr6:1126009-1126028 None:intergenic 40.0%
TTGGTGTATGGAAGTGGAAT+TGG + Chr6:1126655-1126674 MsG0680030366.01.T01:intron 40.0%
! ATACACCAACCTCTAAAAGC+TGG - Chr6:1126644-1126663 None:intergenic 40.0%
! ATCGGTTGAACATTTTGTGG+TGG - Chr6:1125971-1125990 None:intergenic 40.0%
! TACACCAACCTCTAAAAGCT+GGG - Chr6:1126643-1126662 None:intergenic 40.0%
!! CTTGTCATTTCAGGTTCGAT+TGG + Chr6:1126433-1126452 MsG0680030366.01.T01:intron 40.0%
!! GAATTAGGGTTTGTTGGGAT+TGG + Chr6:1125858-1125877 MsG0680030366.01.T01:five_prime_UTR 40.0%
!! GCTTTTGTGCTTGTCATTTC+AGG + Chr6:1126424-1126443 MsG0680030366.01.T01:intron 40.0%
!! GGGTTAGCTTGTATGTTGAT+GGG + Chr6:1126901-1126920 MsG0680030366.01.T01:three_prime_UTR 40.0%
!! TGGCAGGACGAATTTTAATG+TGG + Chr6:1126864-1126883 MsG0680030366.01.T01:intron 40.0%
!! TGGGTTAGCTTGTATGTTGA+TGG + Chr6:1126900-1126919 MsG0680030366.01.T01:three_prime_UTR 40.0%
!!! AGCTTTTAGAGGTTGGTGTA+TGG + Chr6:1126643-1126662 MsG0680030366.01.T01:intron 40.0%
AATGTGGCATGTTCCAAGGT+TGG + Chr6:1126880-1126899 MsG0680030366.01.T01:three_prime_UTR 45.0%
ATGTGGCATGTTCCAAGGTT+GGG + Chr6:1126881-1126900 MsG0680030366.01.T01:three_prime_UTR 45.0%
CAATGTTTACGCTTGCTCAG+AGG - Chr6:1126470-1126489 None:intergenic 45.0%
CATACAAGCTAACCCAACCT+TGG - Chr6:1126896-1126915 None:intergenic 45.0%
CTCTGAGCAAGCGTAAACAT+TGG + Chr6:1126468-1126487 MsG0680030366.01.T01:CDS 45.0%
GAAGCAAGTAGTAGGAATGG+TGG + Chr6:1125896-1125915 MsG0680030366.01.T01:CDS 45.0%
GCACAAATTGTTGTCTCCAG+TGG + Chr6:1126165-1126184 MsG0680030366.01.T01:intron 45.0%
! AATAGTGCTGCTATAGCCAC+TGG - Chr6:1126184-1126203 None:intergenic 45.0%
! GAGAGTTACCCAGCTTTTAG+AGG + Chr6:1126632-1126651 MsG0680030366.01.T01:intron 45.0%
! GGAATGGTAATTTTCGTGGC+AGG + Chr6:1126848-1126867 MsG0680030366.01.T01:intron 45.0%
!! GTTACCCAGCTTTTAGAGGT+TGG + Chr6:1126636-1126655 MsG0680030366.01.T01:intron 45.0%
!! TAGAGGTTGGTGTATGGAAG+TGG + Chr6:1126649-1126668 MsG0680030366.01.T01:intron 45.0%
!!! TTTTTTTTTTTTTTTTTTTG+TGG - Chr6:1126290-1126309 None:intergenic 5.0%
AATGCGAGCTCGAACTCTGT+AGG + Chr6:1126554-1126573 MsG0680030366.01.T01:CDS 50.0%
ATTCTCAGACACCGTTGCAG+AGG - Chr6:1126537-1126556 None:intergenic 50.0%
TTCTCAGACACCGTTGCAGA+GGG - Chr6:1126536-1126555 None:intergenic 50.0%
CCAACTGAGCTAAGCTCACG+AGG - Chr6:1126218-1126237 None:intergenic 55.0%
CCTCGTGAGCTTAGCTCAGT+TGG + Chr6:1126215-1126234 MsG0680030366.01.T01:intron 55.0%
GCGCCGCTGTTTAACATCAC+CGG - Chr6:1126091-1126110 None:intergenic 55.0%
! AAGGTCTGGGGTTCGAATCC+CGG + Chr6:1126261-1126280 MsG0680030366.01.T01:intron 55.0%
Chromosome Type Strat End Strand Name
Chr6 gene 1125836 1127100 1125836 ID=MsG0680030366.01;Name=MsG0680030366.01
Chr6 mRNA 1125836 1127100 1125836 ID=MsG0680030366.01.T01;Parent=MsG0680030366.01;Name=MsG0680030366.01.T01;_AED=0.41;_eAED=0.41;_QI=58|1|1|1|0|0|3|242|103
Chr6 exon 1125836 1126055 1125836 ID=MsG0680030366.01.T01:exon:2398;Parent=MsG0680030366.01.T01
Chr6 five_prime_UTR 1125836 1125893 1125836 ID=MsG0680030366.01.T01:five_prime_utr;Parent=MsG0680030366.01.T01
Chr6 CDS 1125894 1126055 1125894 ID=MsG0680030366.01.T01:cds;Parent=MsG0680030366.01.T01
Chr6 CDS 1126446 1126595 1126446 ID=MsG0680030366.01.T01:cds;Parent=MsG0680030366.01.T01
Chr6 three_prime_UTR 1126596 1126606 1126596 ID=MsG0680030366.01.T01:three_prime_utr;Parent=MsG0680030366.01.T01
Chr6 three_prime_UTR 1126870 1127100 1126870 ID=MsG0680030366.01.T01:three_prime_utr;Parent=MsG0680030366.01.T01
Chr6 mRNA 1125894 1127100 1125894 ID=MsG0680030366.01.T02;Parent=MsG0680030366.01;Name=MsG0680030366.01.T02;_AED=0.42;_eAED=0.42;_QI=0|0.5|0.66|1|0|0|3|242|106
Chr6 exon 1125894 1126064 1125894 ID=MsG0680030366.01.T02:exon:2401;Parent=MsG0680030366.01.T02
Chr6 CDS 1125894 1126064 1125894 ID=MsG0680030366.01.T02:cds;Parent=MsG0680030366.01.T02
Chr6 CDS 1126446 1126595 1126446 ID=MsG0680030366.01.T02:cds;Parent=MsG0680030366.01.T02
Chr6 three_prime_UTR 1126596 1126606 1126596 ID=MsG0680030366.01.T02:three_prime_utr;Parent=MsG0680030366.01.T02
Chr6 three_prime_UTR 1126870 1127100 1126870 ID=MsG0680030366.01.T02:three_prime_utr;Parent=MsG0680030366.01.T02
Chr6 mRNA 1125894 1126094 1125894 ID=MsG0680030366.01.T03;Parent=MsG0680030366.01;Name=MsG0680030366.01.T03;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|0|1|0|66
Chr6 exon 1125894 1126094 1125894 ID=MsG0680030366.01.T03:exon:2402;Parent=MsG0680030366.01.T03
Chr6 CDS 1125894 1126094 1125894 ID=MsG0680030366.01.T03:cds;Parent=MsG0680030366.01.T03
Gene Sequence

>MsG0680030366.01.T02

ATGAAGCAAGTAGTAGGAATGGTGGTTTCGAATAAGATGCAGAAATCGGTTGTGGTAGCAGTTGATAGATTGTTCCACCACAAAATGTTCAACCGATATATTAAACGCACTTCCAAGTTTATGGCTCGTGATGAAAACAATCTTTGCAACATCGGTGACCGAGTTAGTATTGTTCGATTGGATTCTTCTAGGCCTCTGAGCAAGCGTAAACATTGGGTTGTTGCTGAAATTCTCAAGAAAGCACGCATATATGTTTCTCCCTCTGCAACGGTGTCTGAGAATGCGAGCTCGAACTCTGTAGGACCTACATCTACGTCATAA

>MsG0680030366.01.T03

ATGAAGCAAGTAGTAGGAATGGTGGTTTCGAATAAGATGCAGAAATCGGTTGTGGTAGCAGTTGATAGATTGTTCCACCACAAAATGTTCAACCGATATATTAAACGCACTTCCAAGTTTATGGCTCGTGATGAAAACAATCTTTGCAACATCGGTGACCGAGTTAGTATTGTGAATAATTTACCTATCTTTTACCGGTGA

>MsG0680030366.01.T01

ATGAAGCAAGTAGTAGGAATGGTGGTTTCGAATAAGATGCAGAAATCGGTTGTGGTAGCAGTTGATAGATTGTTCCACCACAAAATGTTCAACCGATATATTAAACGCACTTCCAAGTTTATGGCTCGTGATGAAAACAATCTTTGCAACATCGGTGACCGAGTTCGATTGGATTCTTCTAGGCCTCTGAGCAAGCGTAAACATTGGGTTGTTGCTGAAATTCTCAAGAAAGCACGCATATATGTTTCTCCCTCTGCAACGGTGTCTGAGAATGCGAGCTCGAACTCTGTAGGACCTACATCTACGTCATAA

Protein sequence

>MsG0680030366.01.T02

MKQVVGMVVSNKMQKSVVVAVDRLFHHKMFNRYIKRTSKFMARDENNLCNIGDRVSIVRLDSSRPLSKRKHWVVAEILKKARIYVSPSATVSENASSNSVGPTSTS*

>MsG0680030366.01.T03

MKQVVGMVVSNKMQKSVVVAVDRLFHHKMFNRYIKRTSKFMARDENNLCNIGDRVSIVNNLPIFYR*

>MsG0680030366.01.T01

MKQVVGMVVSNKMQKSVVVAVDRLFHHKMFNRYIKRTSKFMARDENNLCNIGDRVRLDSSRPLSKRKHWVVAEILKKARIYVSPSATVSENASSNSVGPTSTS*