AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000026.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000026.01.T01 MTR_0381s0010 95.025 201 10 0 1 201 374 574 3.62e-142 408
MsG0180000026.01.T01 MTR_1g026035 95.025 201 10 0 1 201 588 788 2.06e-139 408
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000026.01.T01 AT2G15820 53.333 195 88 2 7 200 644 836 6.94e-68 222

Find 45 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTATTCTGGATTGGTATTC+TGG 0.195897 1:-473001 MsG0180000026.01.T01:CDS
TACAACTTGGTTCTGGAAAT+TGG 0.196797 1:-472971 MsG0180000026.01.T01:CDS
ACCATTGCAAGCTCTCATTT+TGG 0.235787 1:-473249 MsG0180000026.01.T01:CDS
CAAAATTGAAATGGATAATA+TGG 0.269995 1:+473391 None:intergenic
CACCTTGTGTTCTTGCATAC+TGG 0.289141 1:-473158 MsG0180000026.01.T01:CDS
AACCACCTATGAACAAGTTT+TGG 0.306684 1:+473183 None:intergenic
CTTACATCTTCAAGAATATA+TGG 0.308293 1:+472943 None:intergenic
TCCCTTGACTTTACAATCAA+TGG 0.337999 1:+473032 None:intergenic
AGGGAAAGGGAAAGGTATTC+TGG 0.343457 1:-473014 MsG0180000026.01.T01:CDS
GCAATGGTGCAAAACTTATC+TGG 0.373004 1:+473264 None:intergenic
GGAACTGTAGGTTGACCATT+TGG 0.390599 1:+473204 None:intergenic
GAAGCAATACAACTTGGTTC+TGG 0.398600 1:-472978 MsG0180000026.01.T01:CDS
CAGTTCCAAAACTTGTTCAT+AGG 0.402494 1:-473188 MsG0180000026.01.T01:CDS
AATTGCCATCAAAATTGAAA+TGG 0.413559 1:+473382 None:intergenic
TAAGGGAAGTCGTGAGGGTG+TGG 0.438002 1:-473085 MsG0180000026.01.T01:CDS
GTATTCTGGATTGGTATTCT+GGG 0.446524 1:-473000 MsG0180000026.01.T01:CDS
GATGTAGCCACTCATAAAAC+TGG 0.455806 1:+473322 None:intergenic
GGAGGGCATCGAAATTCATC+GGG 0.486854 1:-473126 MsG0180000026.01.T01:CDS
CTGATTGGGATGCTTCTAGC+TGG 0.494346 1:-473447 None:intergenic
GTCCATTGATTGTAAAGTCA+AGG 0.503337 1:-473034 MsG0180000026.01.T01:CDS
CTTGCATACTGGTACATGTA+TGG 0.514271 1:-473147 MsG0180000026.01.T01:CDS
GATTGTAAAGTCAAGGGAAA+GGG 0.520477 1:-473027 MsG0180000026.01.T01:CDS
AGGTGTGAATATAATGAGAC+AGG 0.525973 1:-472905 MsG0180000026.01.T01:CDS
ATGTTCTCACTGTCACCACT+TGG 0.533528 1:+473288 None:intergenic
TGGAGGGCATCGAAATTCAT+CGG 0.541168 1:-473127 MsG0180000026.01.T01:CDS
AAGGGAAAGGTATTCTGGAT+TGG 0.541846 1:-473009 MsG0180000026.01.T01:CDS
GTAAGAGATTTCACTAAGGC+AGG 0.542553 1:-472925 MsG0180000026.01.T01:CDS
ACCATTTGGCCAAAACTGAT+CGG 0.544895 1:+473218 None:intergenic
TCACCACTTGGCTTAGAAGT+AGG 0.546193 1:+473300 None:intergenic
GACTTTACAATCAATGGACA+TGG 0.551655 1:+473038 None:intergenic
TTCTGGGAAGCAATACAACT+TGG 0.555919 1:-472984 MsG0180000026.01.T01:CDS
TCCATTGATTGTAAAGTCAA+GGG 0.563781 1:-473033 MsG0180000026.01.T01:CDS
TTCCAAAACTTGTTCATAGG+TGG 0.570846 1:-473185 MsG0180000026.01.T01:CDS
TAAAGTCAAGGGAAAGGGAA+AGG 0.573314 1:-473022 MsG0180000026.01.T01:CDS
GCCAAAATGAGAGCTTGCAA+TGG 0.588265 1:+473248 None:intergenic
TGATTGTAAAGTCAAGGGAA+AGG 0.594051 1:-473028 MsG0180000026.01.T01:CDS
GCATACTGGTACATGTATGG+AGG 0.605806 1:-473144 MsG0180000026.01.T01:CDS
AAGATTAAGGGAAGTCGTGA+GGG 0.613821 1:-473090 MsG0180000026.01.T01:CDS
GAAGATTAAGGGAAGTCGTG+AGG 0.622880 1:-473091 MsG0180000026.01.T01:CDS
CATACTGGTACATGTATGGA+GGG 0.627303 1:-473143 MsG0180000026.01.T01:CDS
AGATGTAAGAGATTTCACTA+AGG 0.650087 1:-472929 MsG0180000026.01.T01:CDS
TACCAGTATGCAAGAACACA+AGG 0.651343 1:+473156 None:intergenic
CATCCTACTTCTAAGCCAAG+TGG 0.677886 1:-473303 MsG0180000026.01.T01:CDS
GAGGGCATCGAAATTCATCG+GGG 0.720882 1:-473125 MsG0180000026.01.T01:CDS
AGGGCATCGAAATTCATCGG+GGG 0.752383 1:-473124 MsG0180000026.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAATTGAAATGGATAATA+TGG + Chr1:472905-472924 None:intergenic 20.0%
!!! ATTATCCATTTCAATTTTGA+TGG - Chr1:472906-472925 MsG0180000026.01.T01:CDS 20.0%
! AATTGCCATCAAAATTGAAA+TGG + Chr1:472914-472933 None:intergenic 25.0%
! CTTACATCTTCAAGAATATA+TGG + Chr1:473353-473372 None:intergenic 25.0%
AGATGTAAGAGATTTCACTA+AGG - Chr1:473364-473383 MsG0180000026.01.T01:CDS 30.0%
! GATGTTTTGCTGAAGATTAA+GGG - Chr1:473191-473210 MsG0180000026.01.T01:CDS 30.0%
! TCCATTGATTGTAAAGTCAA+GGG - Chr1:473260-473279 MsG0180000026.01.T01:CDS 30.0%
AGGTGTGAATATAATGAGAC+AGG - Chr1:473388-473407 MsG0180000026.01.T01:CDS 35.0%
GACTTTACAATCAATGGACA+TGG + Chr1:473258-473277 None:intergenic 35.0%
GATTGTAAAGTCAAGGGAAA+GGG - Chr1:473266-473285 MsG0180000026.01.T01:CDS 35.0%
TCCCTTGACTTTACAATCAA+TGG + Chr1:473264-473283 None:intergenic 35.0%
TGATTGTAAAGTCAAGGGAA+AGG - Chr1:473265-473284 MsG0180000026.01.T01:CDS 35.0%
! AACCACCTATGAACAAGTTT+TGG + Chr1:473113-473132 None:intergenic 35.0%
! CAGTTCCAAAACTTGTTCAT+AGG - Chr1:473105-473124 MsG0180000026.01.T01:CDS 35.0%
! GGATGTTTTGCTGAAGATTA+AGG - Chr1:473190-473209 MsG0180000026.01.T01:CDS 35.0%
! GTCCATTGATTGTAAAGTCA+AGG - Chr1:473259-473278 MsG0180000026.01.T01:CDS 35.0%
! TTCCAAAACTTGTTCATAGG+TGG - Chr1:473108-473127 MsG0180000026.01.T01:CDS 35.0%
!! GTATTCTGGATTGGTATTCT+GGG - Chr1:473293-473312 MsG0180000026.01.T01:CDS 35.0%
!! TACAACTTGGTTCTGGAAAT+TGG - Chr1:473322-473341 MsG0180000026.01.T01:CDS 35.0%
!!! TGAACAAGTTTTGGAACTGT+AGG + Chr1:473104-473123 None:intergenic 35.0%
AAGATTAAGGGAAGTCGTGA+GGG - Chr1:473203-473222 MsG0180000026.01.T01:CDS 40.0%
ACCATTTGGCCAAAACTGAT+CGG + Chr1:473078-473097 None:intergenic 40.0%
CATACTGGTACATGTATGGA+GGG - Chr1:473150-473169 MsG0180000026.01.T01:CDS 40.0%
CTTGCATACTGGTACATGTA+TGG - Chr1:473146-473165 MsG0180000026.01.T01:CDS 40.0%
GAAGCAATACAACTTGGTTC+TGG - Chr1:473315-473334 MsG0180000026.01.T01:CDS 40.0%
GATGTAGCCACTCATAAAAC+TGG + Chr1:472974-472993 None:intergenic 40.0%
GCAATGGTGCAAAACTTATC+TGG + Chr1:473032-473051 None:intergenic 40.0%
GTAAGAGATTTCACTAAGGC+AGG - Chr1:473368-473387 MsG0180000026.01.T01:CDS 40.0%
TAAAGTCAAGGGAAAGGGAA+AGG - Chr1:473271-473290 MsG0180000026.01.T01:CDS 40.0%
TACCAGTATGCAAGAACACA+AGG + Chr1:473140-473159 None:intergenic 40.0%
! ACCATTGCAAGCTCTCATTT+TGG - Chr1:473044-473063 MsG0180000026.01.T01:CDS 40.0%
! GCTTTTATGCCGATCAGTTT+TGG - Chr1:473066-473085 MsG0180000026.01.T01:CDS 40.0%
! TTCTGGGAAGCAATACAACT+TGG - Chr1:473309-473328 MsG0180000026.01.T01:CDS 40.0%
!! AAGGGAAAGGTATTCTGGAT+TGG - Chr1:473284-473303 MsG0180000026.01.T01:CDS 40.0%
!! GGTATTCTGGATTGGTATTC+TGG - Chr1:473292-473311 MsG0180000026.01.T01:CDS 40.0%
!! TACATCGCCAGTTTTATGAG+TGG - Chr1:472964-472983 MsG0180000026.01.T01:CDS 40.0%
ATGTTCTCACTGTCACCACT+TGG + Chr1:473008-473027 None:intergenic 45.0%
CACCTTGTGTTCTTGCATAC+TGG - Chr1:473135-473154 MsG0180000026.01.T01:CDS 45.0%
CATCCTACTTCTAAGCCAAG+TGG - Chr1:472990-473009 MsG0180000026.01.T01:CDS 45.0%
GAAGATTAAGGGAAGTCGTG+AGG - Chr1:473202-473221 MsG0180000026.01.T01:CDS 45.0%
GCATACTGGTACATGTATGG+AGG - Chr1:473149-473168 MsG0180000026.01.T01:CDS 45.0%
GCCAAAATGAGAGCTTGCAA+TGG + Chr1:473048-473067 None:intergenic 45.0%
TCACCACTTGGCTTAGAAGT+AGG + Chr1:472996-473015 None:intergenic 45.0%
! GGAACTGTAGGTTGACCATT+TGG + Chr1:473092-473111 None:intergenic 45.0%
!! AGGGAAAGGGAAAGGTATTC+TGG - Chr1:473279-473298 MsG0180000026.01.T01:CDS 45.0%
!! TGGAGGGCATCGAAATTCAT+CGG - Chr1:473166-473185 MsG0180000026.01.T01:CDS 45.0%
!! AGGGCATCGAAATTCATCGG+GGG - Chr1:473169-473188 MsG0180000026.01.T01:CDS 50.0%
!! GAGGGCATCGAAATTCATCG+GGG - Chr1:473168-473187 MsG0180000026.01.T01:CDS 50.0%
!! GCCGATCAGTTTTGGCCAAA+TGG - Chr1:473074-473093 MsG0180000026.01.T01:CDS 50.0%
!! GGAGGGCATCGAAATTCATC+GGG - Chr1:473167-473186 MsG0180000026.01.T01:CDS 50.0%
TAAGGGAAGTCGTGAGGGTG+TGG - Chr1:473208-473227 MsG0180000026.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 472855 473460 472855 ID=MsG0180000026.01;Name=MsG0180000026.01
Chr1 mRNA 472855 473460 472855 ID=MsG0180000026.01.T01;Parent=MsG0180000026.01;Name=MsG0180000026.01.T01;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|201
Chr1 exon 472855 473460 472855 ID=MsG0180000026.01.T01:exon:7829;Parent=MsG0180000026.01.T01
Chr1 CDS 472855 473460 472855 ID=MsG0180000026.01.T01:cds;Parent=MsG0180000026.01.T01
Gene Sequence

>MsG0180000026.01.T01

ATGCTTCTAGCTGGTTTGCAAATTGATTCTGATGACAAAAATAAGAACCATATTATCCATTTCAATTTTGATGGCAATTCTGTGAGTCATTACGTCTTGAAGAGTCATATACATCGCCAGTTTTATGAGTGGCTACATCCTACTTCTAAGCCAAGTGGTGACAGTGAGAACATACCAGATAAGTTTTGCACCATTGCAAGCTCTCATTTTGGCTTTTATGCCGATCAGTTTTGGCCAAATGGTCAACCTACAGTTCCAAAACTTGTTCATAGGTGGTTGTCACCTTGTGTTCTTGCATACTGGTACATGTATGGAGGGCATCGAAATTCATCGGGGGATGTTTTGCTGAAGATTAAGGGAAGTCGTGAGGGTGTGGAGAACATTGTCAAAAAGTTCAAAGCCATGTCCATTGATTGTAAAGTCAAGGGAAAGGGAAAGGTATTCTGGATTGGTATTCTGGGAAGCAATACAACTTGGTTCTGGAAATTGGTTGAGCCATATATTCTTGAAGATGTAAGAGATTTCACTAAGGCAGGTGTGAATATAATGAGACAGGATTTAACAGAAACTCAAGATATTAACTTCTGTAGTGAATCAGACGAATGA

Protein sequence

>MsG0180000026.01.T01

MLLAGLQIDSDDKNKNHIIHFNFDGNSVSHYVLKSHIHRQFYEWLHPTSKPSGDSENIPDKFCTIASSHFGFYADQFWPNGQPTVPKLVHRWLSPCVLAYWYMYGGHRNSSGDVLLKIKGSREGVENIVKKFKAMSIDCKVKGKGKVFWIGILGSNTTWFWKLVEPYILEDVRDFTKAGVNIMRQDLTETQDINFCSESDE*