AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048660.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048660.01.T01 MTR_4g088555 97.087 103 2 1 1 102 1 103 4.70e-67 199
MsG0080048660.01.T01 MTR_2g014200 63.380 71 23 2 33 102 36 104 3.08e-23 88.2
MsG0080048660.01.T01 MTR_8g463140 68.182 44 14 0 59 102 62 105 1.52e-18 77.0
MsG0080048660.01.T01 MTR_8g005960 48.864 88 37 3 27 112 155 236 2.72e-16 73.2
MsG0080048660.01.T01 MTR_2g078770 52.632 57 27 0 46 102 42 98 3.31e-14 65.5
MsG0080048660.01.T01 MTR_7g092930 59.091 44 18 0 59 102 65 108 2.98e-13 65.1
MsG0080048660.01.T01 MTR_3g085180 44.068 59 33 0 44 102 147 205 8.72e-13 63.9
MsG0080048660.01.T01 MTR_3g099080 45.588 68 33 1 39 102 71 138 9.69e-13 63.5
MsG0080048660.01.T01 MTR_1g053715 57.447 47 20 0 56 102 68 114 1.72e-12 62.8
MsG0080048660.01.T01 MTR_7g110320 46.970 66 34 1 47 112 130 194 6.08e-12 61.6
MsG0080048660.01.T01 MTR_7g110320 46.970 66 34 1 47 112 130 194 8.23e-12 61.2
MsG0080048660.01.T01 MTR_2g046550 46.667 60 28 1 47 102 120 179 8.24e-12 61.2
MsG0080048660.01.T01 MTR_7g444860 60.976 41 16 0 62 102 149 189 1.24e-11 60.5
MsG0080048660.01.T01 MTR_1g086250 49.091 55 28 0 48 102 133 187 1.44e-11 60.5
MsG0080048660.01.T01 MTR_1g035010 49.123 57 29 0 46 102 88 144 1.97e-11 60.1
MsG0080048660.01.T01 MTR_7g028740 46.429 56 28 1 47 102 64 117 2.01e-11 59.7
MsG0080048660.01.T01 MTR_8g080680 43.902 82 38 2 29 102 91 172 2.81e-11 59.3
MsG0080048660.01.T01 MTR_5g046670 44.643 56 28 1 50 102 229 284 3.68e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048660.01.T01 AT2G33810 57.627 59 22 1 44 102 39 94 3.65e-17 72.4
MsG0080048660.01.T01 AT1G02065 45.238 84 43 2 40 121 164 246 6.52e-15 68.9
MsG0080048660.01.T01 AT1G02065 49.333 75 35 2 40 112 164 237 8.42e-15 69.3
MsG0080048660.01.T01 AT1G53160 54.386 57 23 1 46 102 41 94 1.37e-13 63.9
MsG0080048660.01.T01 AT1G53160 54.386 57 23 1 46 102 41 94 1.37e-13 63.9
MsG0080048660.01.T01 AT3G60030 50.000 56 28 0 47 102 112 167 1.53e-13 66.2
MsG0080048660.01.T01 AT1G53160 54.386 57 23 1 46 102 41 94 1.66e-13 63.9
MsG0080048660.01.T01 AT2G47070 56.863 51 21 1 62 112 106 155 6.48e-13 64.3
MsG0080048660.01.T01 AT1G27360 52.830 53 25 0 50 102 163 215 1.01e-12 63.5
MsG0080048660.01.T01 AT1G27360 52.830 53 25 0 50 102 163 215 1.01e-12 63.5
MsG0080048660.01.T01 AT1G27360 52.830 53 25 0 50 102 163 215 1.01e-12 63.5
MsG0080048660.01.T01 AT1G27360 52.830 53 25 0 50 102 163 215 1.01e-12 63.5
MsG0080048660.01.T01 AT1G27370 39.683 63 38 0 40 102 154 216 1.24e-12 63.2
MsG0080048660.01.T01 AT3G15270 60.976 41 16 0 62 102 63 103 1.57e-12 61.2
MsG0080048660.01.T01 AT1G27370 43.860 57 32 0 46 102 160 216 1.59e-12 63.2
MsG0080048660.01.T01 AT1G27370 43.860 57 32 0 46 102 160 216 1.59e-12 63.2
MsG0080048660.01.T01 AT1G27370 43.860 57 32 0 46 102 160 216 1.59e-12 63.2
MsG0080048660.01.T01 AT1G27370 43.860 57 32 0 46 102 160 216 1.59e-12 63.2
MsG0080048660.01.T01 AT1G27370 43.860 57 32 0 46 102 160 216 1.59e-12 63.2
MsG0080048660.01.T01 AT1G27370 43.860 57 32 0 46 102 160 216 1.59e-12 63.2
MsG0080048660.01.T01 AT1G69170 50.000 56 25 1 50 102 109 164 1.35e-11 60.5
MsG0080048660.01.T01 AT5G43270 41.250 80 42 1 28 102 130 209 1.77e-11 60.1
MsG0080048660.01.T01 AT5G43270 41.250 80 42 1 28 102 130 209 1.77e-11 60.1
MsG0080048660.01.T01 AT5G43270 41.250 80 42 1 28 102 130 209 1.77e-11 60.1
MsG0080048660.01.T01 AT1G20980 52.830 53 24 1 60 112 118 169 4.00e-11 59.3
MsG0080048660.01.T01 AT5G50670 48.936 47 24 0 56 102 95 141 6.92e-11 58.5
MsG0080048660.01.T01 AT5G50670 48.936 47 24 0 56 102 95 141 6.92e-11 58.5
MsG0080048660.01.T01 AT5G50570 48.936 47 24 0 56 102 95 141 6.92e-11 58.5
MsG0080048660.01.T01 AT5G50570 48.936 47 24 0 56 102 95 141 6.92e-11 58.5

Find 25 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAGGAAGAGGATGGTAGA+AGG 0.346008 contig394end:-8074 MsG0080048660.01.T01:CDS
TGAGGAGGAAGAAGAGGAAG+AGG 0.391649 contig394end:-8109 MsG0080048660.01.T01:CDS
GGAAGAAGAGGAAGAGGATA+CGG 0.438830 contig394end:-8103 MsG0080048660.01.T01:CDS
GCACCTGCCGTACACATTGC+AGG 0.447145 contig394end:-7895 MsG0080048660.01.T01:CDS
AAGAGGATGGTAGAAGGAAG+AGG 0.452598 contig394end:-8068 MsG0080048660.01.T01:CDS
ATGTGAAATATTCTTGTTGA+GGG 0.453314 contig394end:-7746 MsG0080048660.01.T01:CDS
GCAATATCACCGGAGACATA+AGG 0.487344 contig394end:-7938 MsG0080048660.01.T01:CDS
AATGTGTACGGCAGGTGCTT+TGG 0.492016 contig394end:+7900 MsG0080048660.01.T01:intergenic
GGATCATGAGGAGGAAGAAG+AGG 0.499097 contig394end:-8115 MsG0080048660.01.T01:CDS
AGAGGATGGTAGAAGGAAGA+GGG 0.504666 contig394end:-8067 MsG0080048660.01.T01:CDS
ACATTGCAGGATTGCAGCAA+AGG 0.514685 contig394end:-7882 MsG0080048660.01.T01:intron
TATTCCACCTTGTTGTCAAG+TGG 0.526109 contig394end:-7992 MsG0080048660.01.T01:CDS
ACTCACAGACCTTATGTCTC+CGG 0.562455 contig394end:+7929 None:intergenic
CAATTCTCCACTTGACAACA+AGG 0.571704 contig394end:+7985 None:intergenic
CTGCAATCCTGCAATGTGTA+CGG 0.578035 contig394end:+7888 None:intergenic
TTGAAACTATGGAGACAAGA+AGG 0.594631 contig394end:-8170 None:intergenic
AATAAGTTTGAAGTACAAAG+AGG 0.596272 contig394end:-8136 MsG0080048660.01.T01:CDS
GATGTGAAATATTCTTGTTG+AGG 0.596883 contig394end:-7747 MsG0080048660.01.T01:CDS
AATCCTGCAATGTGTACGGC+AGG 0.603274 contig394end:+7892 MsG0080048660.01.T01:intergenic
ATGGAGACAAGAAGGTCAGA+GGG 0.623380 contig394end:-8162 MsG0080048660.01.T01:CDS
GTGAAGCTAAGCAATATCAC+CGG 0.630226 contig394end:-7948 MsG0080048660.01.T01:CDS
TATGGAGACAAGAAGGTCAG+AGG 0.634836 contig394end:-8163 MsG0080048660.01.T01:CDS
GTACAAAGAGGATCATGAGG+AGG 0.636625 contig394end:-8124 MsG0080048660.01.T01:CDS
GAAGTACAAAGAGGATCATG+AGG 0.643502 contig394end:-8127 MsG0080048660.01.T01:CDS
TTCTCCACTTGACAACAAGG+TGG 0.724295 contig394end:+7988 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTACTTAAAAGAGATT+AGG + contig394end:8058-8077 MsG0080048660.01.T01:intergenic 15.0%
!! AATTTACTTAAAAGAGATTA+GGG + contig394end:8057-8076 MsG0080048660.01.T01:intergenic 15.0%
! ATGTGAAATATTCTTGTTGA+GGG - contig394end:8133-8152 MsG0080048660.01.T01:CDS 25.0%
!! AATAAGTTTGAAGTACAAAG+AGG - contig394end:7743-7762 MsG0080048660.01.T01:CDS 25.0%
GATGTGAAATATTCTTGTTG+AGG - contig394end:8132-8151 MsG0080048660.01.T01:CDS 30.0%
AAGAGAAGTTCCAAAAAAGC+TGG - contig394end:7855-7874 MsG0080048660.01.T01:intron 35.0%
!!! ATTTGAGCTTCCAGCTTTTT+TGG + contig394end:7868-7887 MsG0080048660.01.T01:intergenic 35.0%
CAATTCTCCACTTGACAACA+AGG + contig394end:7897-7916 MsG0080048660.01.T01:intergenic 40.0%
GAAGTACAAAGAGGATCATG+AGG - contig394end:7752-7771 MsG0080048660.01.T01:CDS 40.0%
GTGAAGCTAAGCAATATCAC+CGG - contig394end:7931-7950 MsG0080048660.01.T01:CDS 40.0%
! TATTCCACCTTGTTGTCAAG+TGG - contig394end:7887-7906 MsG0080048660.01.T01:CDS 40.0%
!! AGAGGATACGGATTTTGAAG+AGG - contig394end:7788-7807 MsG0080048660.01.T01:intron 40.0%
!! GATTTTGAAGAGGAAGAGGA+TGG - contig394end:7798-7817 MsG0080048660.01.T01:intron 40.0%
!! TACGGATTTTGAAGAGGAAG+AGG - contig394end:7794-7813 MsG0080048660.01.T01:intron 40.0%
AAGAGGAAGAGGATGGTAGA+AGG - contig394end:7805-7824 MsG0080048660.01.T01:intron 45.0%
AAGAGGATGGTAGAAGGAAG+AGG - contig394end:7811-7830 MsG0080048660.01.T01:intron 45.0%
ACATTGCAGGATTGCAGCAA+AGG - contig394end:7997-8016 MsG0080048660.01.T01:CDS 45.0%
ACTCACAGACCTTATGTCTC+CGG + contig394end:7953-7972 MsG0080048660.01.T01:intergenic 45.0%
AGAGGATGGTAGAAGGAAGA+GGG - contig394end:7812-7831 MsG0080048660.01.T01:intron 45.0%
ATGGAGACAAGAAGGTCAGA+GGG - contig394end:7717-7736 MsG0080048660.01.T01:CDS 45.0%
CTGCAATCCTGCAATGTGTA+CGG + contig394end:7994-8013 MsG0080048660.01.T01:intergenic 45.0%
GCAATATCACCGGAGACATA+AGG - contig394end:7941-7960 MsG0080048660.01.T01:CDS 45.0%
GGAAGAAGAGGAAGAGGATA+CGG - contig394end:7776-7795 MsG0080048660.01.T01:intron 45.0%
GTACAAAGAGGATCATGAGG+AGG - contig394end:7755-7774 MsG0080048660.01.T01:CDS 45.0%
TTCTCCACTTGACAACAAGG+TGG + contig394end:7894-7913 MsG0080048660.01.T01:intergenic 45.0%
!! GGTTTTGTCAGCAATGTAGC+AGG - contig394end:8018-8037 MsG0080048660.01.T01:CDS 45.0%
!!! TATATATTTTTGTTTAATTA+AGG - contig394end:8096-8115 MsG0080048660.01.T01:CDS 5.0%
AATCCTGCAATGTGTACGGC+AGG + contig394end:7990-8009 MsG0080048660.01.T01:intergenic 50.0%
AATGTGTACGGCAGGTGCTT+TGG + contig394end:7982-8001 MsG0080048660.01.T01:intergenic 50.0%
GGATCATGAGGAGGAAGAAG+AGG - contig394end:7764-7783 MsG0080048660.01.T01:CDS 50.0%
TGAGGAGGAAGAAGAGGAAG+AGG - contig394end:7770-7789 MsG0080048660.01.T01:intron 50.0%
GCACCTGCCGTACACATTGC+AGG - contig394end:7984-8003 MsG0080048660.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
contig394end gene 7717 8184 7717 ID=MsG0080048660.01;Name=MsG0080048660.01
contig394end mRNA 7717 8184 7717 ID=MsG0080048660.01.T01;Parent=MsG0080048660.01;Name=MsG0080048660.01.T01;_AED=0.46;_eAED=0.56;_QI=0|0|0|1|1|1|2|0|122
contig394end exon 7883 8184 7883 ID=MsG0080048660.01.T01:exon:325;Parent=MsG0080048660.01.T01
contig394end exon 7717 7783 7717 ID=MsG0080048660.01.T01:exon:326;Parent=MsG0080048660.01.T01
contig394end CDS 7883 8184 7883 ID=MsG0080048660.01.T01:cds;Parent=MsG0080048660.01.T01
contig394end CDS 7717 7783 7717 ID=MsG0080048660.01.T01:cds;Parent=MsG0080048660.01.T01
Gene Sequence

>MsG0080048660.01.T01

ATGGAGACAAGAAGGTCAGAGGGAAAAATAAGTTTGAAGTACAAAGAGGATCATGAGGAGGAAGAAGAGGAAGAGGATACGGATTTTGAAGAGGAAGAGGATGGTAGAAGGAAGAGGGTAGTGACAGATCTCTATAGCAAGAGAAGTTCCAAAAAAGCTGGAAGCTCAAATATTCCACCTTGTTGTCAAGTGGAGAATTGTGATGCTGATCTTAGTGAAGCTAAGCAATATCACCGGAGACATAAGGTCTGTGAGTATCATGCCAAAGCACCTGCCGTACACATTGCAGGATTGCAGCAAAGGTTAATCTTTGCTAGATGTGAAATATTCTTGTTGAGGGATTTGATTACTGTTTACACTTTTGTCTAA

Protein sequence

>MsG0080048660.01.T01

METRRSEGKISLKYKEDHEEEEEEEDTDFEEEEDGRRKRVVTDLYSKRSSKKAGSSNIPPCCQVENCDADLSEAKQYHRRHKVCEYHAKAPAVHIAGLQQRLIFARCEIFLLRDLITVYTFV*