AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000381.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000381.01.T01 MTR_1g016900 97.015 134 4 0 54 187 33 166 1.62e-93 270
MsG0180000381.01.T01 MTR_2g068940 70.769 65 10 1 1 56 106 170 2.61e-16 73.9
MsG0180000381.01.T01 MTR_5g037520 57.812 64 19 2 1 56 374 437 1.04e-13 68.9
MsG0180000381.01.T01 MTR_8g022850 62.745 51 12 1 1 44 237 287 3.20e-13 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000381.01.T01 AT4G32930 71.111 135 38 1 54 187 33 167 6.81e-68 205
MsG0180000381.01.T01 AT4G32930 67.133 143 38 2 54 187 33 175 5.34e-65 197

Find 42 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTGCTCTTCTTCTTTCT+TGG 0.165608 1:-5235026 None:intergenic
TTCCTGATTCAGTTGTTTCC+TGG 0.245115 1:-5238734 None:intergenic
ATTCATGATATGCAAGTTCT+AGG 0.250374 1:+5234906 None:intergenic
TCTTGCTCTTCTTCTTTCTT+GGG 0.311351 1:-5235025 None:intergenic
CTCTGATGTTGTTTGACTGC+AGG 0.316066 1:+5238766 MsG0180000381.01.T01:CDS
TACTGTGGCTCAACAAACTA+TGG 0.341597 1:+5234938 MsG0180000381.01.T01:CDS
TCCTGATTCAGTTGTTTCCT+GGG 0.348537 1:-5238733 None:intergenic
AAGGAAACACAAGTTTCTTT+CGG 0.387956 1:-5236190 None:intergenic
ATCAAAAGTAGCACGAAGAT+TGG 0.416177 1:-5239696 None:intergenic
ACCCAGGAAACAACTGAATC+AGG 0.431715 1:+5238732 MsG0180000381.01.T01:CDS
CTTGGGACCACAAGTTGAAA+AGG 0.431962 1:-5235008 None:intergenic
CACTACTCATCTTGTTCAAA+AGG 0.448950 1:+5236246 MsG0180000381.01.T01:CDS
TCTGATGTTGTTTGACTGCA+GGG 0.449314 1:+5238767 MsG0180000381.01.T01:CDS
TGAAGCAAGCAAAGACTTCA+AGG 0.452159 1:+5235058 MsG0180000381.01.T01:CDS
AAGAGGAACGGTGTCGTTCA+AGG 0.495875 1:-5236209 None:intergenic
TGAGTAGTGCCTCTACCAGC+TGG 0.496417 1:-5236232 None:intergenic
TTTGCTGAATATGATGAAAA+GGG 0.508700 1:+5239653 MsG0180000381.01.T01:CDS
GAGTGATTCTTGGAATAACA+TGG 0.518162 1:+5234968 MsG0180000381.01.T01:CDS
GTTCCTCTTCCAGCTGGTAG+AGG 0.531433 1:+5236223 MsG0180000381.01.T01:CDS
TGCAGTCAAACAACATCAGA+GGG 0.551675 1:-5238764 None:intergenic
GGACCACAAGTTGAAAAGGT+TGG 0.553302 1:-5235004 None:intergenic
GTGCCTCTACCAGCTGGAAG+AGG 0.556284 1:-5236226 None:intergenic
CATAGTTACAGTGCTCTCCC+TGG 0.559640 1:-5238685 None:intergenic
GTGCCAAATACAAAATCTAT+CGG 0.564516 1:-5238798 None:intergenic
CGAAGATTGGATATCATGAC+TGG 0.565027 1:-5239683 None:intergenic
TCAGTTGTTTCCTGGGTGAG+TGG 0.574105 1:-5238726 None:intergenic
GAGAATGTTGACTTGTCAAG+TGG 0.584027 1:+5239626 MsG0180000381.01.T01:CDS
CAAAGGAAAACCACTCACCC+AGG 0.587859 1:+5238716 MsG0180000381.01.T01:CDS
GTAACTATGATCCCAGGCAA+AGG 0.590679 1:+5238699 MsG0180000381.01.T01:CDS
TTGTGATGATAGTTGAAATG+TGG 0.592472 1:+5236148 MsG0180000381.01.T01:intron
TCTAGGACTTGTTCCTACTG+TGG 0.600434 1:+5234923 MsG0180000381.01.T01:CDS
AGTGATTCTTGGAATAACAT+GGG 0.600905 1:+5234969 MsG0180000381.01.T01:CDS
GACACCGTTCCTCTTCCAGC+TGG 0.603271 1:+5236217 MsG0180000381.01.T01:CDS
AGAAGAAGAGCAAGAAGCAG+CGG 0.607251 1:+5235033 MsG0180000381.01.T01:CDS
CTGATGTTGTTTGACTGCAG+GGG 0.613153 1:+5238768 MsG0180000381.01.T01:CDS
AGCACTGTAACTATGATCCC+AGG 0.615715 1:+5238693 MsG0180000381.01.T01:CDS
ATAGTTTGTTGAGCCACAGT+AGG 0.622879 1:-5234936 None:intergenic
CTGCAGTCAAACAACATCAG+AGG 0.628425 1:-5238765 None:intergenic
CACTGGATGGAAAGTTGAGT+CGG 0.642523 1:+5238818 MsG0180000381.01.T01:CDS
TCTACCAGCTGGAAGAGGAA+CGG 0.661967 1:-5236221 None:intergenic
TAGTTGAAATGTGGAAGATG+TGG 0.679407 1:+5236157 MsG0180000381.01.T01:intron
GCAGTCAAACAACATCAGAG+GGG 0.773353 1:-5238763 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTTAAAAGTATTGTTT+AGG - Chr1:5235612-5235631 None:intergenic 10.0%
!!! TAATTATTTTCTTGTTTAAA+TGG - Chr1:5235767-5235786 None:intergenic 10.0%
!! AAATAAGATATTGGAAATTT+GGG - Chr1:5238164-5238183 None:intergenic 15.0%
!! AAATCATGTAAATAAGATAT+TGG - Chr1:5238173-5238192 None:intergenic 15.0%
!! TAAATAAGATATTGGAAATT+TGG - Chr1:5238165-5238184 None:intergenic 15.0%
!! TTCTTCTATATATATGATTA+AGG + Chr1:5235208-5235227 MsG0180000381.01.T01:intron 15.0%
!!! ATATTTTTAATCCCATTTTA+AGG + Chr1:5235703-5235722 MsG0180000381.01.T01:intron 15.0%
!!! GTTTATTTGAAATTATCTAT+TGG - Chr1:5236962-5236981 None:intergenic 15.0%
!!! TATTTTTCAATCCTTAAAAT+GGG - Chr1:5235717-5235736 None:intergenic 15.0%
!!! TCTTATTTACATGATTTTTA+TGG + Chr1:5238176-5238195 MsG0180000381.01.T01:intron 15.0%
!!! TTATCTTTTGTTATAGAAAT+AGG + Chr1:5236556-5236575 MsG0180000381.01.T01:intron 15.0%
!!! TTTTTTTTTTAAGCAAAAGA+AGG - Chr1:5235261-5235280 None:intergenic 15.0%
!! AATTGCATTTAGAAGAAAAT+TGG - Chr1:5239576-5239595 None:intergenic 20.0%
!! ACATCAAATTCTTATTAGTT+TGG - Chr1:5239111-5239130 None:intergenic 20.0%
!! ACGGTTAAATTAGAATAATA+AGG + Chr1:5236354-5236373 MsG0180000381.01.T01:intron 20.0%
!! ATCTATAGATACTAAATTGA+TGG + Chr1:5237907-5237926 MsG0180000381.01.T01:intron 20.0%
!! CGGTTAAATTAGAATAATAA+GGG + Chr1:5236355-5236374 MsG0180000381.01.T01:intron 20.0%
!! GTTGAAAATGTATATAATGA+TGG + Chr1:5235394-5235413 MsG0180000381.01.T01:intron 20.0%
!! TAAACAAACACTTATAGTAT+GGG + Chr1:5237378-5237397 MsG0180000381.01.T01:intron 20.0%
!! TATTGCAAACCTAATATTTA+GGG + Chr1:5237558-5237577 MsG0180000381.01.T01:intron 20.0%
!! TTAAACAAACACTTATAGTA+TGG + Chr1:5237377-5237396 MsG0180000381.01.T01:intron 20.0%
!! TTAAGTTTAGGTATTAAAGA+AGG - Chr1:5237032-5237051 None:intergenic 20.0%
!! TTATTGCAAACCTAATATTT+AGG + Chr1:5237557-5237576 MsG0180000381.01.T01:intron 20.0%
!! TTGATTAAAAATTTCTCAGT+TGG - Chr1:5235289-5235308 None:intergenic 20.0%
!!! AATAGACTTAAAAGTATGTT+TGG - Chr1:5238371-5238390 None:intergenic 20.0%
!!! ATAGACTTAAAAGTATGTTT+GGG - Chr1:5238370-5238389 None:intergenic 20.0%
!!! ATTTTTGAATAAGTTGGAAT+TGG + Chr1:5237453-5237472 MsG0180000381.01.T01:intron 20.0%
!!! CTATTTTTCAATCCTTAAAA+TGG - Chr1:5235718-5235737 None:intergenic 20.0%
!!! GCATTAATTTTTGAATAAGT+TGG + Chr1:5237447-5237466 MsG0180000381.01.T01:intron 20.0%
!!! TTGTTTTTTGTTTTTCTTTC+TGG + Chr1:5235125-5235144 MsG0180000381.01.T01:intron 20.0%
!!! TTTGATGTTTTCATTACTTT+TGG + Chr1:5238859-5238878 MsG0180000381.01.T01:intron 20.0%
!!! TTTTATTGTCTTCTCTAATA+TGG + Chr1:5237970-5237989 MsG0180000381.01.T01:intron 20.0%
! AATTGTGTGTTTGGAAAATT+TGG + Chr1:5238240-5238259 MsG0180000381.01.T01:intron 25.0%
! ACCATACATGTACAAAATAT+AGG - Chr1:5238118-5238137 None:intergenic 25.0%
! ACTTTGGATAAGATCTTAAT+GGG - Chr1:5237879-5237898 None:intergenic 25.0%
! AGCTACAATTACTAGTTAAA+CGG - Chr1:5238029-5238048 None:intergenic 25.0%
! AGTGATATATAAACTGGATA+TGG + Chr1:5238927-5238946 MsG0180000381.01.T01:intron 25.0%
! AGTTGAAAAAACTTATAGAC+AGG - Chr1:5237156-5237175 None:intergenic 25.0%
! ATAAAATGTGAATACAAGTG+AGG - Chr1:5237956-5237975 None:intergenic 25.0%
! ATAAGCTAAAAACAACTTAC+AGG - Chr1:5237674-5237693 None:intergenic 25.0%
! ATAGAAAACCTTGAAGAAAT+AGG - Chr1:5236055-5236074 None:intergenic 25.0%
! ATAGATGATATACAAAAGGA+TGG - Chr1:5239431-5239450 None:intergenic 25.0%
! ATTAAAAATTTCTCAGTTGG+AGG - Chr1:5235286-5235305 None:intergenic 25.0%
! ATTAAGATCTTATCCAAAGT+GGG + Chr1:5237879-5237898 MsG0180000381.01.T01:intron 25.0%
! CATACATGTACAAAATATAG+GGG - Chr1:5238116-5238135 None:intergenic 25.0%
! CCATACATGTACAAAATATA+GGG - Chr1:5238117-5238136 None:intergenic 25.0%
! GCTTATAGTGATATATAAAC+TGG + Chr1:5238921-5238940 MsG0180000381.01.T01:intron 25.0%
! GTAAGAATTTCTAACTGAAT+TGG - Chr1:5235890-5235909 None:intergenic 25.0%
! GTTTGAATAACAGCTTAATA+AGG - Chr1:5237830-5237849 None:intergenic 25.0%
! TAGATGATATACAAAAGGAT+GGG - Chr1:5239430-5239449 None:intergenic 25.0%
! TATATAACATTTCCAGAATG+TGG - Chr1:5238535-5238554 None:intergenic 25.0%
! TTAAAAATTTCTCAGTTGGA+GGG - Chr1:5235285-5235304 None:intergenic 25.0%
! TTAAGTCCTTTATCCAAATA+AGG + Chr1:5237297-5237316 MsG0180000381.01.T01:intron 25.0%
! TTGAAGAAATAGGAGAAATT+GGG - Chr1:5236045-5236064 None:intergenic 25.0%
! TTGTTTAGGTATATCATCAT+AGG - Chr1:5235598-5235617 None:intergenic 25.0%
! TTTATAAGTGTGTAACTGTT+TGG + Chr1:5236378-5236397 MsG0180000381.01.T01:intron 25.0%
! TTTGCTGAATATGATGAAAA+GGG + Chr1:5239653-5239672 MsG0180000381.01.T01:CDS 25.0%
!! GATTTTTATGGATCTATCAT+TGG + Chr1:5238188-5238207 MsG0180000381.01.T01:intron 25.0%
!! GTTAGATGTCACATTTTATA+TGG + Chr1:5239008-5239027 MsG0180000381.01.T01:intron 25.0%
!! TAACTGAATTGGAATTTTGA+TGG - Chr1:5235879-5235898 None:intergenic 25.0%
!! TTTCTTGTTTAAATGGATGA+TGG - Chr1:5235760-5235779 None:intergenic 25.0%
!! TTTTGCTGAATATGATGAAA+AGG + Chr1:5239652-5239671 MsG0180000381.01.T01:CDS 25.0%
!!! AAGTGCTATTTTTATTCATG+TGG + Chr1:5238581-5238600 MsG0180000381.01.T01:intron 25.0%
!!! GTCTTATTTGAGTTTATCTA+CGG + Chr1:5236404-5236423 MsG0180000381.01.T01:intron 25.0%
!!! TAGATGATTTTTCTAGTGAT+GGG + Chr1:5239319-5239338 MsG0180000381.01.T01:intron 25.0%
!!! TATTTTTATTCATGTGGATG+TGG + Chr1:5238587-5238606 MsG0180000381.01.T01:intron 25.0%
AAATGGAAATGTGGGAATAA+AGG - Chr1:5239500-5239519 None:intergenic 30.0%
AACACTTATAGTATGGGTAA+AGG + Chr1:5237384-5237403 MsG0180000381.01.T01:intron 30.0%
AAGGAAACACAAGTTTCTTT+CGG - Chr1:5236193-5236212 None:intergenic 30.0%
ACACAATTATTCTACCTAGT+TGG - Chr1:5238228-5238247 None:intergenic 30.0%
ACCTAAAATCAAGTCAAGTT+TGG + Chr1:5238059-5238078 MsG0180000381.01.T01:intron 30.0%
ACCTAAACTTAAGTCCATTT+AGG + Chr1:5237040-5237059 MsG0180000381.01.T01:intron 30.0%
AGATGATATACAAAAGGATG+GGG - Chr1:5239429-5239448 None:intergenic 30.0%
AGGTAGAATAATTGTGTGTT+TGG + Chr1:5238231-5238250 MsG0180000381.01.T01:intron 30.0%
AGTCTATTCCAGAAACAAAA+AGG + Chr1:5238383-5238402 MsG0180000381.01.T01:intron 30.0%
AGTGATTCTTGGAATAACAT+GGG + Chr1:5234969-5234988 MsG0180000381.01.T01:CDS 30.0%
ATAAATTGAAGATCTGACGA+TGG - Chr1:5235472-5235491 None:intergenic 30.0%
CACTTTGGATAAGATCTTAA+TGG - Chr1:5237880-5237899 None:intergenic 30.0%
CATTAAGATCTTATCCAAAG+TGG + Chr1:5237878-5237897 MsG0180000381.01.T01:intron 30.0%
CCTAAACTTAAGTCCATTTA+GGG + Chr1:5237041-5237060 MsG0180000381.01.T01:intron 30.0%
CGCTAACAGAAGAAAAAAAA+TGG + Chr1:5235945-5235964 MsG0180000381.01.T01:intron 30.0%
CTACAATTACTAGTTAAACG+GGG - Chr1:5238027-5238046 None:intergenic 30.0%
CTTGAAGAAATAGGAGAAAT+TGG - Chr1:5236046-5236065 None:intergenic 30.0%
CTTTGGATAAGATCTTAATG+GGG - Chr1:5237878-5237897 None:intergenic 30.0%
GAGAATTTGATTCTAACTCA+TGG + Chr1:5235332-5235351 MsG0180000381.01.T01:intron 30.0%
GAGGATAGATGATATACAAA+AGG - Chr1:5239435-5239454 None:intergenic 30.0%
GCTACAATTACTAGTTAAAC+GGG - Chr1:5238028-5238047 None:intergenic 30.0%
GGGTCATATCAACTAAAATA+AGG - Chr1:5237857-5237876 None:intergenic 30.0%
GTGCCAAATACAAAATCTAT+CGG - Chr1:5238801-5238820 None:intergenic 30.0%
GTTATTAGCTAACTGAGTTT+AGG + Chr1:5238431-5238450 MsG0180000381.01.T01:intron 30.0%
TATTAAAGAAGGTCAACAAC+TGG - Chr1:5237021-5237040 None:intergenic 30.0%
TCGCTGCAGTAAATTTATAA+GGG + Chr1:5237524-5237543 MsG0180000381.01.T01:intron 30.0%
TGAAGAAATAGGAGAAATTG+GGG - Chr1:5236044-5236063 None:intergenic 30.0%
TGTTATAATGTGACGCATTA+TGG - Chr1:5237323-5237342 None:intergenic 30.0%
TGTTCATCTCATATGATTAC+TGG - Chr1:5239177-5239196 None:intergenic 30.0%
TTCGCTGCAGTAAATTTATA+AGG + Chr1:5237523-5237542 MsG0180000381.01.T01:intron 30.0%
TTGATTCAAGAGGGAAAATT+GGG - Chr1:5239277-5239296 None:intergenic 30.0%
TTGTGATGATAGTTGAAATG+TGG + Chr1:5236148-5236167 MsG0180000381.01.T01:intron 30.0%
TTTGGATAAGATCTTAATGG+GGG - Chr1:5237877-5237896 None:intergenic 30.0%
! AAACAGTTGAGTTGCATAAA+TGG + Chr1:5236305-5236324 MsG0180000381.01.T01:intron 30.0%
! AATTTGTATTTTGTAGCTGG+AGG + Chr1:5239592-5239611 MsG0180000381.01.T01:intron 30.0%
! ATAGATTTTGTATTTGGCAC+TGG + Chr1:5238801-5238820 MsG0180000381.01.T01:CDS 30.0%
! ATTTGTATTTTGTAGCTGGA+GGG + Chr1:5239593-5239612 MsG0180000381.01.T01:intron 30.0%
! CAACTTCTTTTTATCCAACT+AGG + Chr1:5238211-5238230 MsG0180000381.01.T01:intron 30.0%
! CAATTTCTCCTATTTCTTCA+AGG + Chr1:5236044-5236063 MsG0180000381.01.T01:intron 30.0%
! CATTTTCAACCCAACAATAT+AGG - Chr1:5235384-5235403 None:intergenic 30.0%
! CCCTATATTTTGTACATGTA+TGG + Chr1:5238114-5238133 MsG0180000381.01.T01:intron 30.0%
! GATGTTTTCATAAACAATCC+TGG + Chr1:5236846-5236865 MsG0180000381.01.T01:intron 30.0%
! TAAAAGTATGTTTGGGTTCA+CGG - Chr1:5238363-5238382 None:intergenic 30.0%
! TAAGGCACTTAAAGCATAAA+CGG - Chr1:5237812-5237831 None:intergenic 30.0%
! TCAATACCTCATGATTTTGT+CGG + Chr1:5235542-5235561 MsG0180000381.01.T01:intron 30.0%
! TGCAATTTGTATTTTGTAGC+TGG + Chr1:5239589-5239608 MsG0180000381.01.T01:intron 30.0%
! TGTCATAAGTTGTTTCCATT+AGG - Chr1:5237649-5237668 None:intergenic 30.0%
!! CTAGATGATTTTTCTAGTGA+TGG + Chr1:5239318-5239337 MsG0180000381.01.T01:intron 30.0%
!! TACTCTATTGTTAGTTTGCT+TGG + Chr1:5238963-5238982 MsG0180000381.01.T01:intron 30.0%
!! TCCAAACTTGACTTGATTTT+AGG - Chr1:5238063-5238082 None:intergenic 30.0%
!! TGAATTGGAATTTTGATGGA+TGG - Chr1:5235875-5235894 None:intergenic 30.0%
!! TTATGGCCTTATTTGGATAA+AGG - Chr1:5237306-5237325 None:intergenic 30.0%
!!! TCAGCTTTTTATTTTCTCGA+GGG + Chr1:5235150-5235169 MsG0180000381.01.T01:intron 30.0%
!!! TTCAGCTTTTTATTTTCTCG+AGG + Chr1:5235149-5235168 MsG0180000381.01.T01:intron 30.0%
AAATGTGGGAATAAAGGATG+GGG - Chr1:5239494-5239513 None:intergenic 35.0%
ACCCAACAATATAGGTGAAA+CGG - Chr1:5235376-5235395 None:intergenic 35.0%
ACCGTTTCACCTATATTGTT+GGG + Chr1:5235372-5235391 MsG0180000381.01.T01:intron 35.0%
AGCATGAAACTTGATTCAAG+AGG - Chr1:5239287-5239306 None:intergenic 35.0%
ATCAAAAGTAGCACGAAGAT+TGG - Chr1:5239699-5239718 None:intergenic 35.0%
ATGTGTGCTAAAAGAGAAAG+AGG + Chr1:5235501-5235520 MsG0180000381.01.T01:intron 35.0%
CAATTCAAACAGACCCTAAA+TGG - Chr1:5237057-5237076 None:intergenic 35.0%
CACTACTCATCTTGTTCAAA+AGG + Chr1:5236246-5236265 MsG0180000381.01.T01:CDS 35.0%
CGCTGCAGTAAATTTATAAG+GGG + Chr1:5237525-5237544 MsG0180000381.01.T01:intron 35.0%
CTAATGCTTATGCTTAAAGC+TGG - Chr1:5239356-5239375 None:intergenic 35.0%
CTATAGATAACAACCCACTT+TGG - Chr1:5237895-5237914 None:intergenic 35.0%
CTCAAAGAAGTGAATCCAAA+CGG - Chr1:5237585-5237604 None:intergenic 35.0%
CTTATTTCCATAAGCTATCC+AGG + Chr1:5237174-5237193 MsG0180000381.01.T01:intron 35.0%
CTTGATTCAAGAGGGAAAAT+TGG - Chr1:5239278-5239297 None:intergenic 35.0%
CTTTAACCTGTATTGTCTAC+TGG + Chr1:5238614-5238633 MsG0180000381.01.T01:intron 35.0%
GAAAATTGGGATTGCATATC+TGG - Chr1:5239264-5239283 None:intergenic 35.0%
GAAATGTGGGAATAAAGGAT+GGG - Chr1:5239495-5239514 None:intergenic 35.0%
GAGTGATTCTTGGAATAACA+TGG + Chr1:5234968-5234987 MsG0180000381.01.T01:CDS 35.0%
GCATGAAACTTGATTCAAGA+GGG - Chr1:5239286-5239305 None:intergenic 35.0%
GGATATAAACACTTGTGAGA+CGG + Chr1:5236425-5236444 MsG0180000381.01.T01:intron 35.0%
GTACATGTATGGTAGAGAAA+AGG + Chr1:5238125-5238144 MsG0180000381.01.T01:intron 35.0%
GTCCATCAAAATGGAAATGT+GGG - Chr1:5239508-5239527 None:intergenic 35.0%
TAAAGAATGCCTAGAACTAG+AGG - Chr1:5239454-5239473 None:intergenic 35.0%
TAGTTGAAATGTGGAAGATG+TGG + Chr1:5236157-5236176 MsG0180000381.01.T01:intron 35.0%
TCTTGCTCTTCTTCTTTCTT+GGG - Chr1:5235028-5235047 None:intergenic 35.0%
TGCACTGCATATCAAACAAA+GGG - Chr1:5238656-5238675 None:intergenic 35.0%
TGCTTAAAGCTGGAAAATAG+AGG - Chr1:5239346-5239365 None:intergenic 35.0%
TTCAGACAGATATGATACCA+TGG - Chr1:5239070-5239089 None:intergenic 35.0%
TTCTTGCTCTTCTTCTTTCT+TGG - Chr1:5235029-5235048 None:intergenic 35.0%
TTGCACTGCATATCAAACAA+AGG - Chr1:5238657-5238676 None:intergenic 35.0%
TTGCTTAAAAGAGTCATAGC+AGG - Chr1:5237425-5237444 None:intergenic 35.0%
TTTATGCCGACAAAATCATG+AGG - Chr1:5235551-5235570 None:intergenic 35.0%
! AACTAGACATGTGTTTTCCA+TGG + Chr1:5239050-5239069 MsG0180000381.01.T01:intron 35.0%
! AGTCTGTTTAAGAGAGCTTT+TGG + Chr1:5237117-5237136 MsG0180000381.01.T01:intron 35.0%
! CAACAATAGAGCTTTTGGAT+TGG - Chr1:5236984-5237003 None:intergenic 35.0%
! CCCTAAATGGACTTAAGTTT+AGG - Chr1:5237044-5237063 None:intergenic 35.0%
! GAGCCGATAGATTTTGTATT+TGG + Chr1:5238795-5238814 MsG0180000381.01.T01:CDS 35.0%
! TTCCCACATTTCCATTTTGA+TGG + Chr1:5239503-5239522 MsG0180000381.01.T01:intron 35.0%
!! ATCATCTATCCTCTAGTTCT+AGG + Chr1:5239442-5239461 MsG0180000381.01.T01:intron 35.0%
!! ATTTTGTATTTGGCACTGGA+TGG + Chr1:5238805-5238824 MsG0180000381.01.T01:CDS 35.0%
!! CTGAATCTCCTTTTTGTTTC+TGG - Chr1:5238394-5238413 None:intergenic 35.0%
!! GCTGTTTTCATAACCTATCT+TGG - Chr1:5237710-5237729 None:intergenic 35.0%
!! TAAGCATTTCTGAGACTGTT+TGG - Chr1:5236937-5236956 None:intergenic 35.0%
!! TTGGAATTTTGATGGATGGA+TGG - Chr1:5235871-5235890 None:intergenic 35.0%
!!! TGGACTTTTTGAGTGATTCT+TGG + Chr1:5234958-5234977 MsG0180000381.01.T01:CDS 35.0%
AAACACTTGTGAGACGGTTT+GGG + Chr1:5236431-5236450 MsG0180000381.01.T01:intron 40.0%
AAAGGATGGGGAAGTAATGT+AGG - Chr1:5239417-5239436 None:intergenic 40.0%
AACCTATCTTGGAGAGCTTA+AGG - Chr1:5237699-5237718 None:intergenic 40.0%
ACAGAACTCAGAAGATGTAG+CGG - Chr1:5235825-5235844 None:intergenic 40.0%
ACGACTGCGAGTAGAAAAAA+CGG + Chr1:5236335-5236354 MsG0180000381.01.T01:intron 40.0%
AGTCTTCTACTCTACTGTCT+CGG + Chr1:5239143-5239162 MsG0180000381.01.T01:intron 40.0%
ATCTCTATGCATTAACACCG+TGG - Chr1:5238322-5238341 None:intergenic 40.0%
ATTTACTGCAGCGAATAGAG+AGG - Chr1:5237518-5237537 None:intergenic 40.0%
CAAAGAGCTAGTAATGCGAA+CGG - Chr1:5235097-5235116 None:intergenic 40.0%
CACCGTTTCACCTATATTGT+TGG + Chr1:5235371-5235390 MsG0180000381.01.T01:intron 40.0%
CCAAACGGACCCTAAATATT+AGG - Chr1:5237570-5237589 None:intergenic 40.0%
CGAAGATTGGATATCATGAC+TGG - Chr1:5239686-5239705 None:intergenic 40.0%
CTTGTTTCAATAAGCTCTCC+AGG - Chr1:5236867-5236886 None:intergenic 40.0%
GAACTATCCTGGATAGCTTA+TGG - Chr1:5237184-5237203 None:intergenic 40.0%
GAAGGTCAACAACTGGAATT+TGG - Chr1:5237014-5237033 None:intergenic 40.0%
GCTAAAAGAGAAAGAGGTAG+TGG + Chr1:5235507-5235526 MsG0180000381.01.T01:intron 40.0%
GGAAATGTGGGAATAAAGGA+TGG - Chr1:5239496-5239515 None:intergenic 40.0%
GGTCCATCAAAATGGAAATG+TGG - Chr1:5239509-5239528 None:intergenic 40.0%
TAAACACTTGTGAGACGGTT+TGG + Chr1:5236430-5236449 MsG0180000381.01.T01:intron 40.0%
TAACCTGTATTGTCTACTGG+TGG + Chr1:5238617-5238636 MsG0180000381.01.T01:intron 40.0%
TACTGACATTGACATCTGTG+TGG + Chr1:5237611-5237630 MsG0180000381.01.T01:intron 40.0%
TACTGTGGCTCAACAAACTA+TGG + Chr1:5234938-5234957 MsG0180000381.01.T01:CDS 40.0%
TATGATTAAGGTTCTGCAGC+AGG + Chr1:5235220-5235239 MsG0180000381.01.T01:intron 40.0%
TCCAAAAGCTCTATTGTTGC+AGG + Chr1:5236985-5237004 MsG0180000381.01.T01:intron 40.0%
TCCAGGATAGTTCGTGAAAA+CGG + Chr1:5237191-5237210 MsG0180000381.01.T01:intron 40.0%
TGAAGCAAGCAAAGACTTCA+AGG + Chr1:5235058-5235077 MsG0180000381.01.T01:CDS 40.0%
TGCAGTCAAACAACATCAGA+GGG - Chr1:5238767-5238786 None:intergenic 40.0%
TTCCAGAATGTGGACATAGA+TGG - Chr1:5238525-5238544 None:intergenic 40.0%
TTCCATCTATGTCCACATTC+TGG + Chr1:5238520-5238539 MsG0180000381.01.T01:intron 40.0%
TTCCTTAAGCTCTCCAAGAT+AGG + Chr1:5237694-5237713 MsG0180000381.01.T01:intron 40.0%
TTGAAGCAGGTCCATCAAAA+TGG - Chr1:5239517-5239536 None:intergenic 40.0%
TTGACCAATCTTCAACCTCA+AGG + Chr1:5236006-5236025 MsG0180000381.01.T01:intron 40.0%
! AAAGAAGTGCAAAGAGTGAG+GGG - Chr1:5235190-5235209 None:intergenic 40.0%
! AGAAAGAAGTGCAAAGAGTG+AGG - Chr1:5235192-5235211 None:intergenic 40.0%
! AGACCAACCTTTTCAACTTG+TGG + Chr1:5235001-5235020 MsG0180000381.01.T01:CDS 40.0%
! CCTAATATTTAGGGTCCGTT+TGG + Chr1:5237567-5237586 MsG0180000381.01.T01:intron 40.0%
! GAAAGAAGTGCAAAGAGTGA+GGG - Chr1:5235191-5235210 None:intergenic 40.0%
! TCCTGATTCAGTTGTTTCCT+GGG - Chr1:5238736-5238755 None:intergenic 40.0%
! TCTGATGTTGTTTGACTGCA+GGG + Chr1:5238767-5238786 MsG0180000381.01.T01:CDS 40.0%
! TTCCTGATTCAGTTGTTTCC+TGG - Chr1:5238737-5238756 None:intergenic 40.0%
!! ATAGTTTGTTGAGCCACAGT+AGG - Chr1:5234939-5234958 None:intergenic 40.0%
!! GAGAATGTTGACTTGTCAAG+TGG + Chr1:5239626-5239645 MsG0180000381.01.T01:CDS 40.0%
!! TCGTTCTCTCTCTTGCTTTT+GGG - Chr1:5235915-5235934 None:intergenic 40.0%
!! TGACGCATTATGGCCTTATT+TGG - Chr1:5237313-5237332 None:intergenic 40.0%
!!! TCTTCGTGCTACTTTTGATG+TGG + Chr1:5239700-5239719 MsG0180000381.01.T01:CDS 40.0%
AAAGGATGGGGAGGTAATGT+AGG - Chr1:5239482-5239501 None:intergenic 45.0%
ACCAATCTTCAACCTCAAGG+TGG + Chr1:5236009-5236028 MsG0180000381.01.T01:intron 45.0%
ACCCAGGAAACAACTGAATC+AGG + Chr1:5238732-5238751 MsG0180000381.01.T01:CDS 45.0%
AGAAGAAGAGCAAGAAGCAG+CGG + Chr1:5235033-5235052 MsG0180000381.01.T01:CDS 45.0%
AGCACTGTAACTATGATCCC+AGG + Chr1:5238693-5238712 MsG0180000381.01.T01:CDS 45.0%
CACTGGATGGAAAGTTGAGT+CGG + Chr1:5238818-5238837 MsG0180000381.01.T01:CDS 45.0%
CATAGAGTTAGCGAAACACG+CGG - Chr1:5238280-5238299 None:intergenic 45.0%
CTGCAGTCAAACAACATCAG+AGG - Chr1:5238768-5238787 None:intergenic 45.0%
GCAAGCAAAGACTTCAAGGT+TGG + Chr1:5235062-5235081 MsG0180000381.01.T01:intron 45.0%
GCAGTCAAACAACATCAGAG+GGG - Chr1:5238766-5238785 None:intergenic 45.0%
GTAACTATGATCCCAGGCAA+AGG + Chr1:5238699-5238718 MsG0180000381.01.T01:CDS 45.0%
GTACCACCAGTAGACAATAC+AGG - Chr1:5238623-5238642 None:intergenic 45.0%
TGCCACAAAAACGTTGAAGC+AGG - Chr1:5239530-5239549 None:intergenic 45.0%
TGGCTGTTTGAGAGACCTAA+TGG + Chr1:5237631-5237650 MsG0180000381.01.T01:intron 45.0%
! AAGAAGTGCAAAGAGTGAGG+GGG - Chr1:5235189-5235208 None:intergenic 45.0%
! AAGCAAAAGAAGGAGTGCAG+AGG - Chr1:5235251-5235270 None:intergenic 45.0%
! AGCAAAAGAAGGAGTGCAGA+GGG - Chr1:5235250-5235269 None:intergenic 45.0%
! CTCGTTCTCTCTCTTGCTTT+TGG - Chr1:5235916-5235935 None:intergenic 45.0%
! CTCTGATGTTGTTTGACTGC+AGG + Chr1:5238766-5238785 MsG0180000381.01.T01:CDS 45.0%
! CTGATGTTGTTTGACTGCAG+GGG + Chr1:5238768-5238787 MsG0180000381.01.T01:CDS 45.0%
! CTGTTGCTTCTACAAGTCCA+CGG + Chr1:5238302-5238321 MsG0180000381.01.T01:intron 45.0%
! CTTGGGACCACAAGTTGAAA+AGG - Chr1:5235011-5235030 None:intergenic 45.0%
! GACCTGCTTCAACGTTTTTG+TGG + Chr1:5239525-5239544 MsG0180000381.01.T01:intron 45.0%
! GCCTGCAACAATAGAGCTTT+TGG - Chr1:5236989-5237008 None:intergenic 45.0%
! GGACCACAAGTTGAAAAGGT+TGG - Chr1:5235007-5235026 None:intergenic 45.0%
! TCTAGGACTTGTTCCTACTG+TGG + Chr1:5234923-5234942 MsG0180000381.01.T01:CDS 45.0%
! TGAGTGGTTTTCCTTTGCCT+GGG - Chr1:5238713-5238732 None:intergenic 45.0%
!! GATGGATGGATGGTTCTAGA+AGG - Chr1:5235861-5235880 None:intergenic 45.0%
CAAAGGAAAACCACTCACCC+AGG + Chr1:5238716-5238735 MsG0180000381.01.T01:CDS 50.0%
CATAGTTACAGTGCTCTCCC+TGG - Chr1:5238688-5238707 None:intergenic 50.0%
GCCACCTTGAGGTTGAAGAT+TGG - Chr1:5236013-5236032 None:intergenic 50.0%
TCTACCAGCTGGAAGAGGAA+CGG - Chr1:5236224-5236243 None:intergenic 50.0%
TGTGGGAATAAAGGATGGGG+AGG - Chr1:5239491-5239510 None:intergenic 50.0%
! AGAAGTGCAAAGAGTGAGGG+GGG - Chr1:5235188-5235207 None:intergenic 50.0%
! GCAAAAGAAGGAGTGCAGAG+GGG - Chr1:5235249-5235268 None:intergenic 50.0%
! GTGAGTGGTTTTCCTTTGCC+TGG - Chr1:5238714-5238733 None:intergenic 50.0%
! TCAGTTGTTTCCTGGGTGAG+TGG - Chr1:5238729-5238748 None:intergenic 50.0%
!! AAGAGGAACGGTGTCGTTCA+AGG - Chr1:5236212-5236231 None:intergenic 50.0%
!! GCAGTGCAAGTTTTGTGCCA+GGG + Chr1:5238668-5238687 MsG0180000381.01.T01:intron 50.0%
!! GCCGTTTTCACGAACTATCC+TGG - Chr1:5237195-5237214 None:intergenic 50.0%
!! TGCAGTGCAAGTTTTGTGCC+AGG + Chr1:5238667-5238686 MsG0180000381.01.T01:intron 50.0%
GTTCCTCTTCCAGCTGGTAG+AGG + Chr1:5236223-5236242 MsG0180000381.01.T01:CDS 55.0%
TGAGTAGTGCCTCTACCAGC+TGG - Chr1:5236235-5236254 None:intergenic 55.0%
GACACCGTTCCTCTTCCAGC+TGG + Chr1:5236217-5236236 MsG0180000381.01.T01:CDS 60.0%
GTGCCTCTACCAGCTGGAAG+AGG - Chr1:5236229-5236248 None:intergenic 60.0%
GGGTCATCGACGCCACCTTG+AGG - Chr1:5236024-5236043 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 5234915 5239730 5234915 ID=MsG0180000381.01;Name=MsG0180000381.01
Chr1 mRNA 5234915 5239730 5234915 ID=MsG0180000381.01.T01;Parent=MsG0180000381.01;Name=MsG0180000381.01.T01;_AED=0.64;_eAED=0.65;_QI=0|0|0|0.75|1|1|4|0|187
Chr1 exon 5234915 5235079 5234915 ID=MsG0180000381.01.T01:exon:28279;Parent=MsG0180000381.01.T01
Chr1 exon 5236160 5236267 5236160 ID=MsG0180000381.01.T01:exon:28278;Parent=MsG0180000381.01.T01
Chr1 exon 5238672 5238839 5238672 ID=MsG0180000381.01.T01:exon:28277;Parent=MsG0180000381.01.T01
Chr1 exon 5239608 5239730 5239608 ID=MsG0180000381.01.T01:exon:28276;Parent=MsG0180000381.01.T01
Chr1 CDS 5234915 5235079 5234915 ID=MsG0180000381.01.T01:cds;Parent=MsG0180000381.01.T01
Chr1 CDS 5236160 5236267 5236160 ID=MsG0180000381.01.T01:cds;Parent=MsG0180000381.01.T01
Chr1 CDS 5238672 5238839 5238672 ID=MsG0180000381.01.T01:cds;Parent=MsG0180000381.01.T01
Chr1 CDS 5239608 5239730 5239608 ID=MsG0180000381.01.T01:cds;Parent=MsG0180000381.01.T01
Gene Sequence

>MsG0180000381.01.T01

ATGCAAGTTCTAGGACTTGTTCCTACTGTGGCTCAACAAACTATGGACTTTTTGAGTGATTCTTGGAATAACATGGGTCAGAAAGAAGACCAACCTTTTCAACTTGTGGTCCCAAGAAAGAAGAAGAGCAAGAAGCAGCGGTCTGAAGCAAGCAAAGACTTCAAGTTGAAATGTGGAAGATGTGGTGAAGTGAGTCCGAAAGAAACTTGTGTTTCCTTGAACGACACCGTTCCTCTTCCAGCTGGTAGAGGCACTACTCATCTTGTTCAAAAGTGCAAGTTTTGTGCCAGGGAGAGCACTGTAACTATGATCCCAGGCAAAGGAAAACCACTCACCCAGGAAACAACTGAATCAGGAAAGTTTTCCCCTCTGATGTTGTTTGACTGCAGGGGCTATGAGCCGATAGATTTTGTATTTGGCACTGGATGGAAAGTTGAGTCGCTGGAGGGAACAAAGTTTGAGAATGTTGACTTGTCAAGTGGAGATTTTGCTGAATATGATGAAAAGGGTGAGTGTCCAGTCATGATATCCAATCTTCGTGCTACTTTTGATGTGGTGAAGTAA
Protein sequence

>MsG0180000381.01.T01

MQVLGLVPTVAQQTMDFLSDSWNNMGQKEDQPFQLVVPRKKKSKKQRSEASKDFKLKCGRCGEVSPKETCVSLNDTVPLPAGRGTTHLVQKCKFCARESTVTMIPGKGKPLTQETTESGKFSPLMLFDCRGYEPIDFVFGTGWKVESLEGTKFENVDLSSGDFAEYDEKGECPVMISNLRATFDVVK*