AlfalfaGEDB | MsG0380017798.01

M. sativa cultivar ZhongmuNo.1 / MsG0380017798.01

KEGG: K20535
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017798.01.T01	XP_013462155.1	98.649	74	1	0	1	74	299	372	3.69E-43	153

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017798.01.T01	Q39021	70.833	72	21	0	1	72	299	370	2.54E-32	117

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017798.01.T01	A0A072VDE0	98.649	74	1	0	1	74	299	372	1.76e-43	153

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000400.01	MsG0380017798.01	0.854667	1.056313e-61	2.377016e-58
MsG0180000595.01	MsG0380017798.01	-0.819098	1.388133e-52	1.058196e-49
MsG0380017798.01	MsG0580024323.01	-0.807554	4.776758e-50	2.668517e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017798.01.T01	MTR_3g112210	98.649	74	1	0	1	74	299	372	4.47e-47	153
MsG0380017798.01.T01	MTR_8g086000	65.714	70	24	0	1	70	299	368	1.41e-28	104
MsG0380017798.01.T01	MTR_8g086000	65.714	70	24	0	1	70	342	411	1.77e-28	105
MsG0380017798.01.T01	MTR_8g086010	61.972	71	26	1	1	70	352	422	4.43e-25	95.9
MsG0380017798.01.T01	MTR_3g460950	57.746	71	29	1	1	70	298	368	4.74e-22	87.4
MsG0380017798.01.T01	MTR_3g060330	57.746	71	29	1	1	70	298	368	4.74e-22	87.4
MsG0380017798.01.T01	MTR_3g460980	54.930	71	31	1	1	70	268	338	6.65e-21	84.0
MsG0380017798.01.T01	MTR_3g060390	54.930	71	31	1	1	70	264	334	7.32e-21	83.6
MsG0380017798.01.T01	MTR_4g061320	47.059	68	35	1	1	67	306	373	4.25e-14	65.5
MsG0380017798.01.T01	MTR_8g098845	43.077	65	36	1	1	64	318	382	3.09e-13	62.8
MsG0380017798.01.T01	MTR_8g028720	46.154	65	34	1	1	64	303	367	3.32e-13	62.8
MsG0380017798.01.T01	MTR_5g010030	43.077	65	36	1	1	64	307	371	2.81e-12	60.1
MsG0380017798.01.T01	MTR_7g078690	41.538	65	37	1	1	64	304	368	7.79e-12	58.9
MsG0380017798.01.T01	MTR_4g087620	41.538	65	37	1	1	64	320	384	1.36e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017798.01.T01	AT1G10210	70.833	72	21	0	1	72	299	370	2.59e-33	117
MsG0380017798.01.T01	AT1G10210	70.833	72	21	0	1	72	299	370	2.59e-33	117
MsG0380017798.01.T01	AT1G10210	70.833	72	21	0	1	72	299	370	2.59e-33	117
MsG0380017798.01.T01	AT2G18170	68.571	70	22	0	1	70	299	368	1.75e-29	107
MsG0380017798.01.T01	AT1G59580	70.588	68	18	1	1	66	299	366	2.71e-23	90.9
MsG0380017798.01.T01	AT1G59580	70.588	68	18	1	1	66	299	366	2.71e-23	90.9
MsG0380017798.01.T01	AT4G36450	56.061	66	28	1	1	65	296	361	3.05e-19	79.7
MsG0380017798.01.T01	AT1G07880	50.000	62	30	1	1	61	299	360	9.42e-14	64.3
MsG0380017798.01.T01	AT4G01370	41.176	68	39	1	1	67	309	376	1.33e-13	63.9
MsG0380017798.01.T01	AT3G59790	43.284	67	37	1	1	66	325	391	5.32e-13	62.4
MsG0380017798.01.T01	AT2G43790	41.538	65	37	1	1	64	328	392	8.53e-12	58.9
MsG0380017798.01.T01	AT1G01560	42.188	64	36	1	1	63	306	369	1.05e-11	58.5

Find 22 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTACCATCCTGAATCTACTA+TGG	0.309116	3:+100761680	MsG0380017798.01.T01:CDS
ATGGCCGGAGGGTCAGAATT+AGG	0.339865	3:-100761579	None:intergenic
AGCGCTTCAACATCCTTTCA+TGG	0.347327	3:+100761542	MsG0380017798.01.T01:CDS
TCATCAATGTCAAGATCAAT+TGG	0.388011	3:-100761606	None:intergenic
CTTGACATTGATGAGGATCT+AGG	0.388345	3:+100761615	MsG0380017798.01.T01:CDS
ATCTAGGGGAAGATGTGATA+AGG	0.411838	3:+100761631	MsG0380017798.01.T01:CDS
GCACTTCCCATAGTAGATTC+AGG	0.412372	3:-100761687	None:intergenic
TCTAGGGGAAGATGTGATAA+GGG	0.446248	3:+100761632	MsG0380017798.01.T01:CDS
GTCAAGATCAATTGGAATGA+TGG	0.482307	3:-100761598	None:intergenic
TTGACATTGATGAGGATCTA+GGG	0.482383	3:+100761616	MsG0380017798.01.T01:CDS
GAAAGGATGTTGAAGCGCTT+CGG	0.492201	3:-100761538	None:intergenic
TGATCCTAATTCTGACCCTC+CGG	0.538059	3:+100761575	MsG0380017798.01.T01:CDS
TACCATCCTGAATCTACTAT+GGG	0.554805	3:+100761681	MsG0380017798.01.T01:CDS
ATGTGATAAGGGAGTTGATG+TGG	0.562621	3:+100761643	MsG0380017798.01.T01:CDS
AGATCAATTGGAATGATGGC+CGG	0.568823	3:-100761594	None:intergenic
AATTGATCTTGACATTGATG+AGG	0.575982	3:+100761608	MsG0380017798.01.T01:CDS
TCATAGAGAGAGGCCATGAA+AGG	0.619890	3:-100761555	None:intergenic
CAATTGGAATGATGGCCGGA+GGG	0.638453	3:-100761590	None:intergenic
TGACATTGATGAGGATCTAG+GGG	0.640284	3:+100761617	MsG0380017798.01.T01:CDS
TTCCCATAGTAGATTCAGGA+TGG	0.642061	3:-100761683	None:intergenic
TCAATTGGAATGATGGCCGG+AGG	0.642347	3:-100761591	None:intergenic
AGAATTAGGATCATAGAGAG+AGG	0.652902	3:-100761565	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AATTGATCTTGACATTGATG+AGG	+	Chr3:100761608-100761627	MsG0380017798.01.T01:CDS	30.0%
	TCATCAATGTCAAGATCAAT+TGG	-	Chr3:100761609-100761628	None:intergenic	30.0%
!!!	TTGTTTTTGATCCCACAAAA+AGG	+	Chr3:100761505-100761524	MsG0380017798.01.T01:CDS	30.0%
	AGAATTAGGATCATAGAGAG+AGG	-	Chr3:100761568-100761587	None:intergenic	35.0%
	GTCAAGATCAATTGGAATGA+TGG	-	Chr3:100761601-100761620	None:intergenic	35.0%
	TACCATCCTGAATCTACTAT+GGG	+	Chr3:100761681-100761700	MsG0380017798.01.T01:CDS	35.0%
	TTACCATCCTGAATCTACTA+TGG	+	Chr3:100761680-100761699	MsG0380017798.01.T01:CDS	35.0%
	TTGACATTGATGAGGATCTA+GGG	+	Chr3:100761616-100761635	MsG0380017798.01.T01:CDS	35.0%
	AGATCAATTGGAATGATGGC+CGG	-	Chr3:100761597-100761616	None:intergenic	40.0%
	ATCTAGGGGAAGATGTGATA+AGG	+	Chr3:100761631-100761650	MsG0380017798.01.T01:CDS	40.0%
	CTTGACATTGATGAGGATCT+AGG	+	Chr3:100761615-100761634	MsG0380017798.01.T01:CDS	40.0%
	TCTAGGGGAAGATGTGATAA+GGG	+	Chr3:100761632-100761651	MsG0380017798.01.T01:CDS	40.0%
	TGACATTGATGAGGATCTAG+GGG	+	Chr3:100761617-100761636	MsG0380017798.01.T01:CDS	40.0%
	TTCCCATAGTAGATTCAGGA+TGG	-	Chr3:100761686-100761705	None:intergenic	40.0%
!	GTGACACTGATCCTTTTTGT+GGG	-	Chr3:100761519-100761538	None:intergenic	40.0%
!!	ATGTGATAAGGGAGTTGATG+TGG	+	Chr3:100761643-100761662	MsG0380017798.01.T01:CDS	40.0%
	AGCGCTTCAACATCCTTTCA+TGG	+	Chr3:100761542-100761561	MsG0380017798.01.T01:CDS	45.0%
	GCACTTCCCATAGTAGATTC+AGG	-	Chr3:100761690-100761709	None:intergenic	45.0%
	TCATAGAGAGAGGCCATGAA+AGG	-	Chr3:100761558-100761577	None:intergenic	45.0%
	TGATCCTAATTCTGACCCTC+CGG	+	Chr3:100761575-100761594	MsG0380017798.01.T01:CDS	45.0%
!	GAAAGGATGTTGAAGCGCTT+CGG	-	Chr3:100761541-100761560	None:intergenic	45.0%
!	GGTGACACTGATCCTTTTTG+TGG	-	Chr3:100761520-100761539	None:intergenic	45.0%
	CAATTGGAATGATGGCCGGA+GGG	-	Chr3:100761593-100761612	None:intergenic	50.0%
	TCAATTGGAATGATGGCCGG+AGG	-	Chr3:100761594-100761613	None:intergenic	50.0%
	ATGGCCGGAGGGTCAGAATT+AGG	-	Chr3:100761582-100761601	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	100761501	100761725	100761501	ID=MsG0380017798.01;Name=MsG0380017798.01
Chr3	mRNA	100761501	100761725	100761501	ID=MsG0380017798.01.T01;Parent=MsG0380017798.01;Name=MsG0380017798.01.T01;_AED=0.46;_eAED=0.46;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|74
Chr3	exon	100761501	100761725	100761501	ID=MsG0380017798.01.T01:exon:19465;Parent=MsG0380017798.01.T01
Chr3	CDS	100761501	100761725	100761501	ID=MsG0380017798.01.T01:cds;Parent=MsG0380017798.01.T01

Gene Sequence	>MsG0380017798.01.T01 ATGCTTGTTTTTGATCCCACAAAAAGGATCAGTGTCACCGAAGCGCTTCAACATCCTTTCATGGCCTCTCTCTATGATCCTAATTCTGACCCTCCGGCCATCATTCCAATTGATCTTGACATTGATGAGGATCTAGGGGAAGATGTGATAAGGGAGTTGATGTGGAGTGAAATGCTTCATTACCATCCTGAATCTACTATGGGAAGTGCAGAGTTGTGCTCTTGA
Protein sequence	>MsG0380017798.01.T01 MLVFDPTKRISVTEALQHPFMASLYDPNSDPPAIIPIDLDIDEDLGEDVIRELMWSEMLHYHPESTMGSAELCS*